Lsi03G011400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G011400
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGDSL esterase/lipase
Locationchr03: 21640961 .. 21646488 (+)
RNA-Seq ExpressionLsi03G011400
SyntenyLsi03G011400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTATTGTCATATGATATAGTGCTATATAGTTCTGAATAGTTTCGTGTTTGGCTATACTTCGGAATGAAAATATAATTAAAATAATTTGTGTTATAGGACCCAATTATCCTTTCTATTTTAAGCTGAACTTTGAGACGAGATCGAGGAAGAAGAAGGGGATCCTGGGGACTGATTGAATCAGAAACTAAGTAATGGCAGGCCAGAATGAGCAAGGGAAATCTGCATCTACGGAACAGTTTGTATACAGAATCAGTACATCCAAGGAGTGGGATGAATCACAGCGCGTTGGTCATATCTATGGAGGAGAGCTCGACAAGTCTTCCGGTTTCATTCATCTCAGTAACCTCAATCAGGTTCTTTCTCTAATCTCATCGGACTCTCTACTTTCCAGTCGTATAGAAGAATTTTGCTCTTTCCACAAACGATATTGCATTATATTCAGGAGGAATCGTGATTTCAGCTTGCATTCTGTTTGTTATTTGATTTGCGTATTTCATACACTTTCTTTCGGTTCCTCCCATCTAGGTTAATCCTTGTCTTCATTCCTAGATTTTTTGTTAATTGCTTATTTTAAAGTGGGTTGTGTTCATAGATTGTATTACAACCTTCGAACAACTTTTTTCATGCTACACTTATCGATTCTAAGCGGTTAATTAAGCTACCAAACTGACGTAAAAGTAGTTTGCAAGGTGAAATTGATAGCCAGTTTAGAGAAATCGAATGTAGTTTCTCCGAGATTTAATTGATGGGCATGGCAATATCCTAAAATCAATATTCTTCAGCTAATTTGATTGCCCAGTCAAATCTTATGCATTGGTAAGCATTATTTGAAAACCTTAAATTTAGGAGTGAACCAAAGATTAAATCACCCCATGATGAATTGTTTAACAGTGAGTCTTGGAACTGGTTTATGTCTTGCAGGTCCAGTCAACTTTGCAGAATTTCTTCCTGAATATCAAAGATGAATTGTATCTCCTTCAAATTGAGGTCAAAAAGGTACTGAAACACCTTTTCTTTTCCCTCTCGTGTCTGGAATTGTGCAATGAATATAAAAGGGGAGAGCATAAATTTCTTGTGATTTTGAACTGTCCTATTGCTCTACATGTGCATGTTTTGATTTTGTGATATGATGAACAACTATTACAATGGAAAGATTAAAGGAGACTTGGCTTCAAAAAGTGTTATTGTGCGAGCTAGCATTATAACCATCTAGTTTGTAGACTCCACCACGCAACACAATGGATATACGTATTACTGTATTAGCGAATTATATCCTTCATTAAATATCATTGGTTTAAGGCCCTGGCTATACTCCAAAACTCTGGCTCGTATGGAAAATGTTCTTAAGAATGCTTTCCCGGTACTCAAGAGGCATGTTCTATTATTGAAAGTTAGCTTCATAGTCATTCGGTTTGACAAGATGAATCGTGAAATGAAACAAACTAAGTTCATATGGTCCCTGACTCCCTGTACTTTCAAATGCCTAAATTTTGTCCTTGTACTTTCAATAAAACATAAAATGAGTCCTTAGTTTATGTTTGTTTGAAGTGAATTTTTATAGAACTTGATCAACAACAATAATAATTTCTGCTCAAGAAAAAACACAATGAGAGTATGTTCCCAAAATTTATGAGTGAAGGCTAGTGGAGATTATTTTTTCTAACAGAAAAGTCTAACACTAAACCAACAGTAGAGACTAGTTTTAGGACTTATTAAAAGTGCAGGGACTAAATTTGGATATTTGAAAATATAGACTACAATTGAACAAGTCTCTAACTACATGGACCAAAATAGTACTTATACCAAAATTTTATTATCACCAACAGTTGACGGAGTCTTTGGCCTATTTTTTCCATTTTACATGTTATCAGTGTTTGGCCTTTTTTCAATTTTACATGTTCTTACATTGTCTATATTTGGTATTACATTAACCTTTTTCTTTGCAGCTAGGTGATGGATTGATTTATGAACTTGTGGATGGTTCCAATAGCTTCCCTCATTTCTATGGTCCGTCGCGAAGCTTCAGCCCTATCCCTATGGATGCAGTAACCAAAGCAGAAAAACTCACTTTTTCTGATGGTCGATTTGCTTGTAGTTTTCTCGAATAGGAATAGCTTTAGTTGGCCATTCCAACTTTGTATTGTATCAACAAACCAATTCCTGCTCAAGGTTATAGGCTGCAGAAGCCAAGCGCCTACAATACTGCAGCCTCTTTCTTTTTCTAATTTGTAAAATACATTGGAGCCTTAAATTCATATGAAGCTTTTGGGTAGTTATCTGCTGTAACTGAGTCGTCCTATTATATATAGTACTTCATATCCAAGAGCCACACTGCAGAATATTAAAAACGATCAATGGTTAATGCCTTCATTTATCAGGCAAAAATAGGAATAGAAATGATCACTTGCGATTGTGGAGATATATATTATATATCAATATTTTGTGTCGAGATGTTATTTTTCCATTTATAGTTCAGATGTCCATGTAATCACTTCCTGGTATCCATGTTACTAAGATGCTCATGTTCTCTTCGAATATAAGGATGTTATGGAAGATCTTATTTGTATGAATTCCTTTCCACATGCTTTTCTTATATATTTTCTCCAGAAAATTTCCAGGTAGCTTGTACTGGGTAATTGAACTGGGAGATGCTGGTACGTGTGTTGTCTTAATATTATATTTTAGTGGCATTATTATATTTCATCATGATGTTCATGTGACATTCACTAATTGACATCAAGTTGCTAAATGTCGGTAGTCACTGTATGGACAAAACTGAGGTTCTTGTCACAGACTAAGAGAAAACGACAGTTTTTGTGATACTCAGGATAAGATTCATCTAACTCACTCTCTCTCTGATTTCTCCAGGGATCTGTTACTCACGGACTAGCATAAGGAATAGTGCGAGCCCAAAATAAAACAAAATAAGGAAACTATATTCAACATCTTTTTGTGCTTTCTCACGCTTGACGACAAGATTTGCAGTTTCTCTCCACCATCCTGCTACAAGTTAAAGCAACTTACCACCAACTTGCATTAGAACCGGAGAAAATTTCCCATAACGGACAGAAATTTTAGTTTCTTGGAAGTAATGTCCCTACGCTCTCTCTTTAAACGTTTGTTGTAGGAAGAATTATATGGATCTTGTTATTTTTCATTCTGGTTAGTGGTCAGTTGGATGGATGAAAATGGAGGCTGTTCATTAATTTAATTGGGCCAAAACGTCTTTAATCTTATAAATCGTGGAAGACAATAAAAGATCATATCAGATTTTTTTAGAATTTTTAATTTGGCTTCTTGTGAATTCAAGAGGCTTTGAGTGCTTAACTAGCTGAGACGCAAGGAAATCTCAAACAGAGCACATGGAGCGCAACTGGGATCTTTAGCTCTCACTACATATGACATTGTCTCTCTGCTCTTTATTTATTGTGATCATTCGTTACCATAAACAAGCGGGTTAATGAATCTGATTACAGAAATGGCGGAAAAGAAGAGGAATTCCTTTCCTTTTCCATTGTTTGTCATCTTCTTCCTACTATCTCTACCTGGTCCTCTTTTTGTTCATTCCCAGTGCACCCAAAAGCCAGTTATTTTCAACTTCGGTGACTCGAATTCTGATACAGGTGGTTTCTCTGAGGGACTTGGCCTCAAATTTGGACTCCCAACTGGCCGTACATTCTTCCATAAACCATCAGGAAGATTATGTGACGGACGTCTAATGATTGATTTTCTATGTATGTACTCTGATCCAACAAAACAAATTCTCAATAGCTTGATTTTCTTACTAAAATGCATTTTCTTCCTCAGGTGAAAGTGTGAGTTCTGATTATTTGACCCCATATCTGCGATCTGTGGGCCCAAATTTCACCAATGGAGCCAACTTTGCTATATCTGGTTCAGCTACTCTGCCAAAGGATCGTCCATTTAATTTGCATATTCAAATTATGCAATTCCTTCAATTTCAATCACGCTCCCTTGAGCTAATTCCAAAAGGTATATCTCGTTCAATATAGTTGTTGATTAAACACACATCCTTACCTGATTTCTCTACAATTTTTAATGGATGGGAAGACTCTCTTCATTCTACTATTGACTAAGGTTTCTAAGCCAAGTGAACTGATGTAGGCTATAAAGATTTGGTTGATGAAGAGGGCTTCAATAATGCACTTTACACAATTGACATCGGACAAAATGATCTTGCTGCCGCATTCACTTACTTGTCTTATCCGCAAGTCATCCAGAAAATCCCATCTTTTGTTTCTGAGATAAAAAATGCCATATGGGTTAGCCTTCAATTTCTGCACTGTTGAATTACTACTTACCACCTAAAAATTTTAAGCTAATAGGTCATGGAAAATCTTTTAATTTTTCTCAACACTCCACCCCATGTGTTCGACTCAAATTTTAGTAGATATAAAATTTGATAAAAAATTGACATAATATAAGTTTGAAGTTTGCCCTGAAGTTTAGTTAGCTCTCCATTTTCAGTGTATTCTTCTGGCCTTGAAAGCAGAAGTTGAAGAAGAAAAGTCAATGGATTCCTGTGTTTTTGCTTTTGCTACTCCTCCTTAATTGCTTTTCAATCCAAATGCAGGATATATATGAACATGGAGGAAGAAATTTTTGGGTAGACAACACAGGGCCATTGGGCTGTTTACCTCAAAAGCTTGCTGCAACTTCTGCAAATGCCAGTGACTTTGACAATCATGGATGCCTCCAATCCCTCAACAATGCTGCAAAAGAGTTCAACACTCAGTTGAAAGCTGCATGTGGAGAACTAAGATCTGCTTTGAAAAATGCCACCATTGTGTATGTTGACATCTACGCCATCAAATACGAACTCATAGCCAACTCCATCTCGAACGGTATTGCCAATTATCTTCACCATCTTCTAACAAGAACCAACAATATTATCATCTTGACTCATGCTGTTTCCTACATTGTGCAGGGTTTGAGAATCCATTGATGGTGTGTTGTGGCTATGGAGGACCGCCATACAACTTCAACCAGAGCGTTACTTGCGGCCAACCGGGCTTCAACACGTGCAACGAAGGATTGAAGTATTTAAGTTGGGATGGTGTTCACTATACAGAAGCAGCAAATGCTGTGTTTGCCTCCAAGATACTTTCTTCTCAATACTCTTCTCCAAAACTTCACTTTAATTTCTTCTGCAACAAGTGATGGTTGAATTCATTTTGACCCAACTTAAAAAGGTCATATAATTTGGATAATCTCCAGCTTTAGATTGGAGTTCTGTAGGACGGTTGCTCTTTTTACTGCGTTATCGAGAGTATTGGCAAAACTGTAATCAAGGAAAAGTAAGGGGTGCCATACAAAAACTTATTCATAAAGAAAATCATAAAAAGCAAACATTTCAAAGTTATCAACGGTACATGTTATTCTTTTAAGTTTGTACTAAAAAAACATGTTCTTTTTTGTTTTTATTTTTACTATATATATATATATATATATATATATGGAGTAAGAGCATCGAACCTCAAATTTTAAAGTTGATAGTACAATTTCAT

mRNA sequence

GTATTGTCATATGATATAGTGCTATATAGTTCTGAATAGTTTCGTGTTTGGCTATACTTCGGAATGAAAATATAATTAAAATAATTTGTGTTATAGGACCCAATTATCCTTTCTATTTTAAGCTGAACTTTGAGACGAGATCGAGGAAGAAGAAGGGGATCCTGGGGACTGATTGAATCAGAAACTAAGTAATGGCAGGCCAGAATGAGCAAGGGAAATCTGCATCTACGGAACAGTTTGTATACAGAATCAGTACATCCAAGGAGTGGGATGAATCACAGCGCGTTGGTCATATCTATGGAGGAGAGCTCGACAAGTCTTCCGGTTTCATTCATCTCAGTAACCTCAATCAGGTCCAGTCAACTTTGCAGAATTTCTTCCTGAATATCAAAGATGAATTGTATCTCCTTCAAATTGAGGTCAAAAAGCTAGGTGATGGATTGATTTATGAACTTGTGGATGGTTCCAATAGCTTCCCTCATTTCTATGGTCCGTCGCGAAGCTTCAGCCCTATCCCTATGGATGCATGCACCCAAAAGCCAGTTATTTTCAACTTCGGTGACTCGAATTCTGATACAGGTGGTTTCTCTGAGGGACTTGGCCTCAAATTTGGACTCCCAACTGGCCGTACATTCTTCCATAAACCATCAGGAAGATTATGTGACGGACGTCTAATGATTGATTTTCTATGTGAAAGTGTGAGTTCTGATTATTTGACCCCATATCTGCGATCTGTGGGCCCAAATTTCACCAATGGAGCCAACTTTGCTATATCTGGTTCAGCTACTCTGCCAAAGGATCGTCCATTTAATTTGCATATTCAAATTATGCAATTCCTTCAATTTCAATCACGCTCCCTTGAGCTAATTCCAAAAGGCTATAAAGATTTGGTTGATGAAGAGGGCTTCAATAATGCACTTTACACAATTGACATCGGACAAAATGATCTTGCTGCCGCATTCACTTACTTGTCTTATCCGCAAGTCATCCAGAAAATCCCATCTTTTGTTTCTGAGATAAAAAATGCCATATGGGATATATATGAACATGGAGGAAGAAATTTTTGGGTAGACAACACAGGGCCATTGGGCTGTTTACCTCAAAAGCTTGCTGCAACTTCTGCAAATGCCAGTGACTTTGACAATCATGGATGCCTCCAATCCCTCAACAATGCTGCAAAAGAGTTCAACACTCAGTTGAAAGCTGCATGTGGAGAACTAAGATCTGCTTTGAAAAATGCCACCATTGTGTATGTTGACATCTACGCCATCAAATACGAACTCATAGCCAACTCCATCTCGAACGGGTTTGAGAATCCATTGATGGTGTGTTGTGGCTATGGAGGACCGCCATACAACTTCAACCAGAGCGTTACTTGCGGCCAACCGGGCTTCAACACGTGCAACGAAGGATTGAAGTATTTAAGTTGGGATGGTGTTCACTATACAGAAGCAGCAAATGCTGTGTTTGCCTCCAAGATACTTTCTTCTCAATACTCTTCTCCAAAACTTCACTTTAATTTCTTCTGCAACAAGTGATGGTTGAATTCATTTTGACCCAACTTAAAAAGGTCATATAATTTGGATAATCTCCAGCTTTAGATTGGAGTTCTGTAGGACGGTTGCTCTTTTTACTGCGTTATCGAGAGTATTGGCAAAACTGTAATCAAGGAAAAGTAAGGGGTGCCATACAAAAACTTATTCATAAAGAAAATCATAAAAAGCAAACATTTCAAAGTTATCAACGGTACATGTTATTCTTTTAAGTTTGTACTAAAAAAACATGTTCTTTTTTGTTTTTATTTTTACTATATATATATATATATATATATATATGGAGTAAGAGCATCGAACCTCAAATTTTAAAGTTGATAGTACAATTTCAT

Coding sequence (CDS)

ATGGCAGGCCAGAATGAGCAAGGGAAATCTGCATCTACGGAACAGTTTGTATACAGAATCAGTACATCCAAGGAGTGGGATGAATCACAGCGCGTTGGTCATATCTATGGAGGAGAGCTCGACAAGTCTTCCGGTTTCATTCATCTCAGTAACCTCAATCAGGTCCAGTCAACTTTGCAGAATTTCTTCCTGAATATCAAAGATGAATTGTATCTCCTTCAAATTGAGGTCAAAAAGCTAGGTGATGGATTGATTTATGAACTTGTGGATGGTTCCAATAGCTTCCCTCATTTCTATGGTCCGTCGCGAAGCTTCAGCCCTATCCCTATGGATGCATGCACCCAAAAGCCAGTTATTTTCAACTTCGGTGACTCGAATTCTGATACAGGTGGTTTCTCTGAGGGACTTGGCCTCAAATTTGGACTCCCAACTGGCCGTACATTCTTCCATAAACCATCAGGAAGATTATGTGACGGACGTCTAATGATTGATTTTCTATGTGAAAGTGTGAGTTCTGATTATTTGACCCCATATCTGCGATCTGTGGGCCCAAATTTCACCAATGGAGCCAACTTTGCTATATCTGGTTCAGCTACTCTGCCAAAGGATCGTCCATTTAATTTGCATATTCAAATTATGCAATTCCTTCAATTTCAATCACGCTCCCTTGAGCTAATTCCAAAAGGCTATAAAGATTTGGTTGATGAAGAGGGCTTCAATAATGCACTTTACACAATTGACATCGGACAAAATGATCTTGCTGCCGCATTCACTTACTTGTCTTATCCGCAAGTCATCCAGAAAATCCCATCTTTTGTTTCTGAGATAAAAAATGCCATATGGGATATATATGAACATGGAGGAAGAAATTTTTGGGTAGACAACACAGGGCCATTGGGCTGTTTACCTCAAAAGCTTGCTGCAACTTCTGCAAATGCCAGTGACTTTGACAATCATGGATGCCTCCAATCCCTCAACAATGCTGCAAAAGAGTTCAACACTCAGTTGAAAGCTGCATGTGGAGAACTAAGATCTGCTTTGAAAAATGCCACCATTGTGTATGTTGACATCTACGCCATCAAATACGAACTCATAGCCAACTCCATCTCGAACGGGTTTGAGAATCCATTGATGGTGTGTTGTGGCTATGGAGGACCGCCATACAACTTCAACCAGAGCGTTACTTGCGGCCAACCGGGCTTCAACACGTGCAACGAAGGATTGAAGTATTTAAGTTGGGATGGTGTTCACTATACAGAAGCAGCAAATGCTGTGTTTGCCTCCAAGATACTTTCTTCTCAATACTCTTCTCCAAAACTTCACTTTAATTTCTTCTGCAACAAGTGA

Protein sequence

MAGQNEQGKSASTEQFVYRISTSKEWDESQRVGHIYGGELDKSSGFIHLSNLNQVQSTLQNFFLNIKDELYLLQIEVKKLGDGLIYELVDGSNSFPHFYGPSRSFSPIPMDACTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQSRSLELIPKGYKDLVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNAIWDIYEHGGRNFWVDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSALKNATIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKYLSWDGVHYTEAANAVFASKILSSQYSSPKLHFNFFCNK
Homology
BLAST of Lsi03G011400 vs. ExPASy Swiss-Prot
Match: O80522 (GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 4.1e-115
Identity = 204/339 (60.18%), Postives = 254/339 (74.93%), Query Frame = 0

Query: 113 CTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSS 172
           C   PVIFNFGDSNSDTGG   GLG   GLP GR+FF + +GRL DGRL+IDFLC+S+++
Sbjct: 32  CQVPPVIFNFGDSNSDTGGLVAGLGYSIGLPNGRSFFQRSTGRLSDGRLVIDFLCQSLNT 91

Query: 173 DYLTPYLRS-VGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQSRSLEL--IPKG 232
             L PYL S VG  F NGANFAI GS+TLP+  PF L+IQ+MQFL F+SR+LEL  I   
Sbjct: 92  SLLNPYLDSLVGSKFQNGANFAIVGSSTLPRYVPFALNIQLMQFLHFKSRALELASISDP 151

Query: 233 YKD-LVDEEGFNNALYTIDIGQNDLAAAFTY-LSYPQVIQKIPSFVSEIKNAIWDIYEHG 292
            K+ ++ E GF NALY IDIGQND+A +F+  LSY +V++ IP+ +SEIK+AI  +Y+ G
Sbjct: 152 LKEMMIGESGFRNALYMIDIGQNDIADSFSKGLSYSRVVKLIPNVISEIKSAIKILYDEG 211

Query: 293 GRNFWVDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSAL 352
           GR FWV NTGPLGCLPQKL  +  ++  FD HGCL + N AAK FN  L   C +LR+ L
Sbjct: 212 GRKFWVHNTGPLGCLPQKL--SMVHSKGFDKHGCLATYNAAAKLFNEGLDHMCRDLRTEL 271

Query: 353 KNATIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGFNTCNEG 412
           K A IVYVDIYAIKY+LIANS + GFE PLM CCGYGGPPYN+N ++TCG  G  +C+EG
Sbjct: 272 KEANIVYVDIYAIKYDLIANSNNYGFEKPLMACCGYGGPPYNYNVNITCGNGGSKSCDEG 331

Query: 413 LKYLSWDGVHYTEAANAVFASKILSSQYSSPKLHFNFFC 447
            +++SWDG+HYTE ANA+ A K+LS Q+S+P   F+FFC
Sbjct: 332 SRFISWDGIHYTETANAIVAMKVLSMQHSTPPTPFHFFC 368

BLAST of Lsi03G011400 vs. ExPASy Swiss-Prot
Match: Q9FXB6 (GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 8.6e-113
Identity = 200/368 (54.35%), Postives = 251/368 (68.21%), Query Frame = 0

Query: 92  SNSFPHFYGPSRSFS------PIPMDACTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTG 151
           SN+F  F+    S +      P    +CT +PVIFNFGDSNSDTGG   GLG   G P G
Sbjct: 8   SNTFSFFFITLVSLALLILRQPSRAASCTARPVIFNFGDSNSDTGGLVAGLGYPIGFPNG 67

Query: 152 RTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVG-PNFTNGANFAISGSATLPKDR 211
           R FF + +GRL DGRL+IDFLC+S+++  L PYL S+G   F NGANFAI+GS TLPK+ 
Sbjct: 68  RLFFRRSTGRLSDGRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSPTLPKNV 127

Query: 212 PFNLHIQIMQFLQFQSRSLELIPKGYK---DLVDEEGFNNALYTIDIGQNDLAAAFTY-L 271
           PF+L+IQ+ QF  F+SRSLEL           +   GF NALY IDIGQND+A +F    
Sbjct: 128 PFSLNIQVKQFSHFKSRSLELASSSNSLKGMFISNNGFKNALYMIDIGQNDIARSFARGN 187

Query: 272 SYPQVIQKIPSFVSEIKNAIWDIYEHGGRNFWVDNTGPLGCLPQKLAATSANASDFDNHG 331
           SY Q ++ IP  ++EIK++I  +Y+ GGR FW+ NTGPLGCLPQKL  +   + D D HG
Sbjct: 188 SYSQTVKLIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKL--SMVKSKDLDQHG 247

Query: 332 CLQSLNNAAKEFNTQLKAACGELRSALKNATIVYVDIYAIKYELIANSISNGFENPLMVC 391
           CL S N+AA  FN  L   C ELR+ L++ATI+Y+DIYAIKY LIANS   GF++PLM C
Sbjct: 248 CLVSYNSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQYGFKSPLMAC 307

Query: 392 CGYGGPPYNFNQSVTCGQPGFNTCNEGLKYLSWDGVHYTEAANAVFASKILSSQYSSPKL 449
           CGYGG PYN+N  +TCG  G N C EG +++SWDG+HYTE ANA+ A K+LS  YS P  
Sbjct: 308 CGYGGTPYNYNVKITCGHKGSNVCEEGSRFISWDGIHYTETANAIVAMKVLSMHYSKPPT 367

BLAST of Lsi03G011400 vs. ExPASy Swiss-Prot
Match: Q6NLP7 (GDSL esterase/lipase At3g62280 OS=Arabidopsis thaliana OX=3702 GN=At3g62280 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.2e-101
Identity = 181/327 (55.35%), Postives = 226/327 (69.11%), Query Frame = 0

Query: 115 QKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSSDY 174
           +KP++ NFGDSNSDTGG   G+GL  GLP G TFFH+ +GRL DGRL++DF CE +   Y
Sbjct: 33  KKPILINFGDSNSDTGGVLAGVGLPIGLPHGITFFHRGTGRLGDGRLIVDFYCEHLKMTY 92

Query: 175 LTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQSRSLELIPKGYKDLV 234
           L+PYL S+ PNF  G NFA+SG+  LP    F L IQI QF+ F++RS ELI  G +DL+
Sbjct: 93  LSPYLDSLSPNFKRGVNFAVSGATALP-IFSFPLAIQIRQFVHFKNRSQELISSGRRDLI 152

Query: 235 DEEGFNNALYTIDIGQNDLAAAF--TYLSYPQVIQKIPSFVSEIKNAIWDIYEHGGRNFW 294
           D+ GF NALY IDIGQNDL  A   + L+Y  V++KIPS + EIK AI  +Y +GGR FW
Sbjct: 153 DDNGFRNALYMIDIGQNDLLLALYDSNLTYAPVVEKIPSMLLEIKKAIQTVYLYGGRKFW 212

Query: 295 VDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSALKNATI 354
           V NTGPLGC P++LA    N SD D  GC +  N  AK FN  L + C ELRS  K+AT+
Sbjct: 213 VHNTGPLGCAPKELAIHLHNDSDLDPIGCFRVHNEVAKAFNKGLLSLCNELRSQFKDATL 272

Query: 355 VYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKYLS 414
           VYVDIY+IKY+L A+    GF +PLM CCGYGG P N+++  TCGQPG   C +  K + 
Sbjct: 273 VYVDIYSIKYKLSADFKLYGFVDPLMACCGYGGRPNNYDRKATCGQPGSTICRDVTKAIV 332

Query: 415 WDGVHYTEAANAVFASKILSSQYSSPK 440
           WDGVHYTEAAN      +L+++YS PK
Sbjct: 333 WDGVHYTEAANRFVVDAVLTNRYSYPK 358

BLAST of Lsi03G011400 vs. ExPASy Swiss-Prot
Match: Q3ECP6 (GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.8e-79
Identity = 157/365 (43.01%), Postives = 210/365 (57.53%), Query Frame = 0

Query: 117 PVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLT 176
           P  FNFGDSNSDTG    GLG++  LP G+  F   S R CDGRL+IDFL + +   +L 
Sbjct: 30  PSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQRFCDGRLVIDFLMDEMDLPFLN 89

Query: 177 PYLRSVG-PNFTNGANFAISGSATLPKD----RPFNLHIQIMQFLQFQSRSLELIPKG-- 236
           PYL S+G PNF  G NFA +GS  LP +     PF+  +QI QF++F+SR++EL+ K   
Sbjct: 90  PYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDLQISQFIRFKSRAIELLSKTGR 149

Query: 237 --YKDLVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNAIWDIYEHG 296
              K L   + ++  LY IDIGQND+A AF   +  QV+  IPS +   +  +  +YE G
Sbjct: 150 KYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVLASIPSILETFEAGLKRLYEEG 209

Query: 297 GRNFWVDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSAL 356
           GRN W+ NTGPLGCL Q +A    +++  D  GC+ S N AAK FN QL A   + ++  
Sbjct: 210 GRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSSHNQAAKLFNLQLHAMSNKFQAQY 269

Query: 357 KNATIVYVDIYAIKYELIA------------------------NSISN--GFENPLMVCC 416
            +A + YVDI++IK  LIA                        N I N  GFE PLM CC
Sbjct: 270 PDANVTYVDIFSIKSNLIANYSRFGKHFTKPLIDLNHLENVGYNKILNVLGFEKPLMACC 329

Query: 417 GYGGPPYNFNQSVTCGQPGF--------NTCNEGLKYLSWDGVHYTEAANAVFASKILSS 439
           G GG P N++  +TCGQ             CN+  +Y++WDG+HYTEAAN   +S+IL+ 
Sbjct: 330 GVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTG 389

BLAST of Lsi03G011400 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.4e-78
Identity = 156/335 (46.57%), Postives = 211/335 (62.99%), Query Frame = 0

Query: 117 PVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLT 176
           P IFNFGDSNSDTGG S   G + G P G +FF  P+GR CDGRL+IDF+ ES+   YL+
Sbjct: 29  PAIFNFGDSNSDTGGLSAAFG-QAGPPHGSSFFGSPAGRYCDGRLVIDFIAESLGLPYLS 88

Query: 177 PYLRSVGPNFTNGANFAISGS------ATLPKD--RPFNLHIQIMQFLQFQSRSLELIPK 236
            +L SVG NF++GANFA +GS      +TL +    PF+L +Q +QF  F +RS  +  +
Sbjct: 89  AFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLDVQFVQFYNFHNRSQTVRSR 148

Query: 237 G--YKDLVDE-EGFNNALYTIDIGQNDLAAA-FTYLSYPQVIQKIPSFVSEIKNAIWDIY 296
           G  YK ++ E + F+ ALYT DIGQNDL A  F   +  QV  ++P  +S+  NAI +IY
Sbjct: 149 GGVYKTMLPESDSFSKALYTFDIGQNDLTAGYFANKTVEQVETEVPEIISQFMNAIKNIY 208

Query: 297 EHGGRNFWVDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELR 356
             GGR FW+ NTGP+GCL   +      ASDFD+HGC+  LN+ A++FN  LK A  ELR
Sbjct: 209 GQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHGCVSPLNHLAQQFNHALKQAVIELR 268

Query: 357 SALKNATIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGF--- 416
           S+L  A I YVD+Y++K+EL  ++  +GF+  L+ CCG+GG  YN+N+ + CG       
Sbjct: 269 SSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCGHGG-KYNYNKGIGCGMKKIVKG 328

Query: 417 ------NTCNEGLKYLSWDGVHYTEAANAVFASKI 431
                   C+E  K + WDGVH+T+AAN     KI
Sbjct: 329 KEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKI 361

BLAST of Lsi03G011400 vs. ExPASy TrEMBL
Match: A0A0A0L9E0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G669600 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 8.8e-185
Identity = 308/338 (91.12%), Postives = 323/338 (95.56%), Query Frame = 0

Query: 113 CTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSS 172
           C QKPVIFNFGDSNSDTGGFSEGLG+KFGLPTGRTFFHKP+GRLCDGRLMIDFLCESV+S
Sbjct: 38  CIQKPVIFNFGDSNSDTGGFSEGLGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNS 97

Query: 173 DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQSRSLELIPKGYKD 232
           DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNL+IQIMQFLQFQSRSLELIPKGYKD
Sbjct: 98  DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKGYKD 157

Query: 233 LVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNAIWDIYEHGGRNFW 292
           LVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQ+IPSFVSEIKNAIW IY+HGGRNFW
Sbjct: 158 LVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQQIPSFVSEIKNAIWTIYQHGGRNFW 217

Query: 293 VDNTGPLGCLPQKLAA--TSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSALKNA 352
           + NTGPLGCLPQKLA     ANASD DNHGCLQS NNAAKEFNTQL+ ACGELRSAL NA
Sbjct: 218 IHNTGPLGCLPQKLATAYADANASDIDNHGCLQSFNNAAKEFNTQLRVACGELRSALTNA 277

Query: 353 TIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKY 412
           T+VYVD+YAIKY+LI NS+SNGFENPL+VCCGYGGPPYNFNQ+VTCGQPGFNTCNEGLKY
Sbjct: 278 TLVYVDVYAIKYDLITNSVSNGFENPLIVCCGYGGPPYNFNQTVTCGQPGFNTCNEGLKY 337

Query: 413 LSWDGVHYTEAANAVFASKILSSQYSSPKLHFNFFCNK 449
           +SWDGVHYTEAANAVFAS ILSSQYSSPKL FNFFCNK
Sbjct: 338 VSWDGVHYTEAANAVFASMILSSQYSSPKLPFNFFCNK 375

BLAST of Lsi03G011400 vs. ExPASy TrEMBL
Match: A0A1S3BHH9 (GDSL esterase/lipase At1g09390-like OS=Cucumis melo OX=3656 GN=LOC103489900 PE=3 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 4.8e-183
Identity = 305/337 (90.50%), Postives = 323/337 (95.85%), Query Frame = 0

Query: 113 CTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSS 172
           C QKPVIFNFGDSNSDTGG SEGLG+KFGLPTGRTFFHKP+GRLCDGRLMIDFLCESVSS
Sbjct: 38  CIQKPVIFNFGDSNSDTGGLSEGLGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVSS 97

Query: 173 DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQSRSLELIPKGYKD 232
           DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNL++QIMQFLQFQSRSLELIPKGYKD
Sbjct: 98  DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLYVQIMQFLQFQSRSLELIPKGYKD 157

Query: 233 LVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNAIWDIYEHGGRNFW 292
           LVDEEGFNNALYTIDIGQNDLAAAFTYL Y QVIQKIPSFVSEIK+AIW++Y+HGGR FW
Sbjct: 158 LVDEEGFNNALYTIDIGQNDLAAAFTYLPYSQVIQKIPSFVSEIKDAIWNLYQHGGRKFW 217

Query: 293 VDNTGPLGCLPQKLAA--TSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSALKNA 352
           + NTGPLGCLPQKLAA   +ANASD DNHGCLQSLNNAAKEFNTQL+ ACGELRSAL N 
Sbjct: 218 IHNTGPLGCLPQKLAAAYANANASDIDNHGCLQSLNNAAKEFNTQLRVACGELRSALTNV 277

Query: 353 TIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKY 412
           TIVYVD+YAIKY++IANS+SNGFENPL+VCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKY
Sbjct: 278 TIVYVDVYAIKYDIIANSVSNGFENPLIVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKY 337

Query: 413 LSWDGVHYTEAANAVFASKILSSQYSSPKLHFNFFCN 448
           +SWDGVHYTEAANAVFASKILSSQYSSPKL FNFFC+
Sbjct: 338 VSWDGVHYTEAANAVFASKILSSQYSSPKLPFNFFCS 374

BLAST of Lsi03G011400 vs. ExPASy TrEMBL
Match: A0A5A7T9T8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00720 PE=3 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 2.0e-181
Identity = 305/340 (89.71%), Postives = 323/340 (95.00%), Query Frame = 0

Query: 113 CTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSS 172
           C QKPVIFNFGDSNSDTGG SEGLG+KFGLPTGRTFFHKP+GRLCDGRLMIDFLCESVSS
Sbjct: 32  CIQKPVIFNFGDSNSDTGGLSEGLGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVSS 91

Query: 173 DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQSRSLELIPKG--- 232
           DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNL++QIMQFLQFQSRSLELIPKG   
Sbjct: 92  DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLYVQIMQFLQFQSRSLELIPKGIPR 151

Query: 233 YKDLVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNAIWDIYEHGGR 292
           YKDLVDEEGFNNALYTIDIGQNDLAAAFTYL Y QVIQKIPSFVSEIK+AIW++Y+HGGR
Sbjct: 152 YKDLVDEEGFNNALYTIDIGQNDLAAAFTYLPYSQVIQKIPSFVSEIKDAIWNLYQHGGR 211

Query: 293 NFWVDNTGPLGCLPQKLAA--TSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSAL 352
            FW+ NTGPLGCLPQKLAA   +ANASD DNHGCLQSLNNAAKEFNTQL+ ACGELRSAL
Sbjct: 212 KFWIHNTGPLGCLPQKLAAAYANANASDIDNHGCLQSLNNAAKEFNTQLRVACGELRSAL 271

Query: 353 KNATIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGFNTCNEG 412
            N TIVYVD+YAIKY++IANS+SNGFENPL+VCCGYGGPPYNFNQSVTCGQPGFNTCNEG
Sbjct: 272 TNVTIVYVDVYAIKYDIIANSVSNGFENPLIVCCGYGGPPYNFNQSVTCGQPGFNTCNEG 331

Query: 413 LKYLSWDGVHYTEAANAVFASKILSSQYSSPKLHFNFFCN 448
           LKY+SWDGVHYTEAANAVFASKILSSQYSSPKL FNFFC+
Sbjct: 332 LKYVSWDGVHYTEAANAVFASKILSSQYSSPKLPFNFFCS 371

BLAST of Lsi03G011400 vs. ExPASy TrEMBL
Match: A0A6J1KA99 (GDSL esterase/lipase At1g09390-like OS=Cucurbita maxima OX=3661 GN=LOC111493087 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 1.9e-179
Identity = 302/360 (83.89%), Postives = 321/360 (89.17%), Query Frame = 0

Query: 93  NSFPH-----FYGPSRSFSPIPMDACTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRT 152
           NSFP      F+  S     + +  C+QKPVIFNFGDSNSDTGGFSEGLGL+FG P+GR 
Sbjct: 7   NSFPFPLFVIFFLLSLPGPLLVLSQCSQKPVIFNFGDSNSDTGGFSEGLGLRFGPPSGRV 66

Query: 153 FFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFN 212
           FFHKPSGRLCDGRLMIDFLCESV SDYLTPYLRSVG NFTNG NFAISGSATLPKDRPFN
Sbjct: 67  FFHKPSGRLCDGRLMIDFLCESVGSDYLTPYLRSVGSNFTNGVNFAISGSATLPKDRPFN 126

Query: 213 LHIQIMQFLQFQSRSLELIPKGYKDLVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQ 272
           L+IQ+MQFLQFQSRSLELIPKGYKDLVDEEGFNNA+YTIDIGQNDLA +FTYLSY QVI 
Sbjct: 127 LYIQVMQFLQFQSRSLELIPKGYKDLVDEEGFNNAIYTIDIGQNDLAGSFTYLSYSQVIH 186

Query: 273 KIPSFVSEIKNAIWDIYEHGGRNFWVDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNN 332
           +IPSFV+EIKNAIW IY+HGGRNFWV NTGPLGCLPQKLA  S NASD DNHGCLQSLNN
Sbjct: 187 RIPSFVTEIKNAIWTIYQHGGRNFWVHNTGPLGCLPQKLATASTNASDIDNHGCLQSLNN 246

Query: 333 AAKEFNTQLKAACGELRSALKNATIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPP 392
           AAKEFNT LKAACGELRSAL NATIVYVD+YAIKY+LIAN++ NGFENP   CCGYGGPP
Sbjct: 247 AAKEFNTLLKAACGELRSALMNATIVYVDVYAIKYDLIANAVLNGFENPSTACCGYGGPP 306

Query: 393 YNFNQSVTCGQPGFNTCNEGLKYLSWDGVHYTEAANAVFASKILSSQYSSPKLHFNFFCN 448
           YNFNQSVTCG PGFN CNEGLKY+SWDGVHYTEAAN VFASKILSSQYSSPKLHFN+FCN
Sbjct: 307 YNFNQSVTCGAPGFNVCNEGLKYVSWDGVHYTEAANGVFASKILSSQYSSPKLHFNYFCN 366

BLAST of Lsi03G011400 vs. ExPASy TrEMBL
Match: W9R6R2 (GDSL esterase/lipase OS=Morus notabilis OX=981085 GN=L484_018784 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 5.9e-173
Identity = 306/526 (58.17%), Postives = 366/526 (69.58%), Query Frame = 0

Query: 8   GKSASTEQFVYRISTSKEWDESQRVGHIYGGELDKSSGFIHLSNLNQVQSTLQNFFLNIK 67
           G     E+FVYRIST++EW+E Q+ G  +GG+LDKSSGFIHLS+L+QV+STLQNFFLN  
Sbjct: 4   GGREEEEEFVYRISTAQEWEELQKSGSTFGGQLDKSSGFIHLSSLSQVRSTLQNFFLNTN 63

Query: 68  DELYLLQIEVKKLGDGLIYELVDGSNSFPHFYGPSRSFSPIPMDA--------------- 127
            ELYLLQI+ KKLGDGL+YE+VDGSNSFPHFYGPSRSFSPIP+D                
Sbjct: 64  HELYLLQIDAKKLGDGLVYEVVDGSNSFPHFYGPSRSFSPIPLDVVTKAEKLSLLDVLDF 123

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 124 QYIAGIAQLGERQTEDLKVACSIHAHRISFCNLEKKEAVQENRGIEETYKPNFGSFGNKP 183

Query: 188 -------------CTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDG 247
                        C +KPVIFNFGDSNSDTGGFS G G  FG PTGR FFH P+GRLCDG
Sbjct: 184 QVGLPQVVNSTSECNRKPVIFNFGDSNSDTGGFSLGSGFTFGPPTGRAFFHHPTGRLCDG 243

Query: 248 RLMIDFLCESVSSDYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQ 307
           RLMIDFLCES++ DYLTPY++S+GPNFT GANFA+SG++T P+ +PFNL  QI QFLQF+
Sbjct: 244 RLMIDFLCESLNMDYLTPYMQSLGPNFTYGANFAVSGASTQPERKPFNLDGQIRQFLQFR 303

Query: 308 SRSLELIPKGYKDLVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNA 367
           +RS+ELI KGYKDLV  E F N+LYTIDIGQNDLAA+FTYLSY QVI KIPSF++EI+ +
Sbjct: 304 TRSIELISKGYKDLVGGEEFINSLYTIDIGQNDLAASFTYLSYSQVIDKIPSFIAEIQTS 363

Query: 368 IWDIYEHGGRNFWVDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNNAAKEFNTQLKAA 427
           I  +Y++G +NFWV NTGPLGCLPQ L+ T+ +ASD DN+GC+Q +N AAK FN QL A 
Sbjct: 364 IQTLYQNGAKNFWVHNTGPLGCLPQILSVTNHSASDIDNYGCIQYMNEAAKAFNKQLYAL 423

Query: 428 CGELRSALKNATIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQP 446
             +LRS +K+ATIV+VDIY+IKY +I+N    GFENPLM CCGYGGPPYN+  +  CG P
Sbjct: 424 YEQLRSEMKDATIVHVDIYSIKYGVISNPSIYGFENPLMACCGYGGPPYNYKLNHACGVP 483

BLAST of Lsi03G011400 vs. NCBI nr
Match: XP_038905016.1 (GDSL esterase/lipase At1g09390-like [Benincasa hispida])

HSP 1 Score: 677.2 bits (1746), Expect = 9.9e-191
Identity = 318/336 (94.64%), Postives = 329/336 (97.92%), Query Frame = 0

Query: 113 CTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSS 172
           CTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCE+VSS
Sbjct: 38  CTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCENVSS 97

Query: 173 DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQSRSLELIPKGYKD 232
           DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPF+LHIQIMQFLQFQSRSLELIPKGYKD
Sbjct: 98  DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFSLHIQIMQFLQFQSRSLELIPKGYKD 157

Query: 233 LVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNAIWDIYEHGGRNFW 292
           LVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNAIW+IYEHGG+ FW
Sbjct: 158 LVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNAIWNIYEHGGKKFW 217

Query: 293 VDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSALKNATI 352
           V NTGPLGCLPQKLAATS+NASD DNHGCLQS NNAAKEFN QLKAACGELRSAL NATI
Sbjct: 218 VHNTGPLGCLPQKLAATSSNASDLDNHGCLQSQNNAAKEFNAQLKAACGELRSALTNATI 277

Query: 353 VYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKYLS 412
           VYVD+YAIKY+LIANS+SNGFENPL+VCCGYGGPPYNFNQS+TCGQPGFNTCNEGLKY+S
Sbjct: 278 VYVDVYAIKYDLIANSVSNGFENPLIVCCGYGGPPYNFNQSITCGQPGFNTCNEGLKYVS 337

Query: 413 WDGVHYTEAANAVFASKILSSQYSSPKLHFNFFCNK 449
           WDGVHYTEAANAVFASKILSSQYS PKLHFNFFCNK
Sbjct: 338 WDGVHYTEAANAVFASKILSSQYSFPKLHFNFFCNK 373

BLAST of Lsi03G011400 vs. NCBI nr
Match: XP_004150835.1 (GDSL esterase/lipase At1g09390 [Cucumis sativus] >KGN58540.1 hypothetical protein Csa_001237 [Cucumis sativus])

HSP 1 Score: 656.4 bits (1692), Expect = 1.8e-184
Identity = 308/338 (91.12%), Postives = 323/338 (95.56%), Query Frame = 0

Query: 113 CTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSS 172
           C QKPVIFNFGDSNSDTGGFSEGLG+KFGLPTGRTFFHKP+GRLCDGRLMIDFLCESV+S
Sbjct: 38  CIQKPVIFNFGDSNSDTGGFSEGLGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNS 97

Query: 173 DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQSRSLELIPKGYKD 232
           DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNL+IQIMQFLQFQSRSLELIPKGYKD
Sbjct: 98  DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKGYKD 157

Query: 233 LVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNAIWDIYEHGGRNFW 292
           LVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQ+IPSFVSEIKNAIW IY+HGGRNFW
Sbjct: 158 LVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQQIPSFVSEIKNAIWTIYQHGGRNFW 217

Query: 293 VDNTGPLGCLPQKLAA--TSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSALKNA 352
           + NTGPLGCLPQKLA     ANASD DNHGCLQS NNAAKEFNTQL+ ACGELRSAL NA
Sbjct: 218 IHNTGPLGCLPQKLATAYADANASDIDNHGCLQSFNNAAKEFNTQLRVACGELRSALTNA 277

Query: 353 TIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKY 412
           T+VYVD+YAIKY+LI NS+SNGFENPL+VCCGYGGPPYNFNQ+VTCGQPGFNTCNEGLKY
Sbjct: 278 TLVYVDVYAIKYDLITNSVSNGFENPLIVCCGYGGPPYNFNQTVTCGQPGFNTCNEGLKY 337

Query: 413 LSWDGVHYTEAANAVFASKILSSQYSSPKLHFNFFCNK 449
           +SWDGVHYTEAANAVFAS ILSSQYSSPKL FNFFCNK
Sbjct: 338 VSWDGVHYTEAANAVFASMILSSQYSSPKLPFNFFCNK 375

BLAST of Lsi03G011400 vs. NCBI nr
Match: XP_008447456.1 (PREDICTED: GDSL esterase/lipase At1g09390-like [Cucumis melo])

HSP 1 Score: 650.6 bits (1677), Expect = 9.9e-183
Identity = 305/337 (90.50%), Postives = 323/337 (95.85%), Query Frame = 0

Query: 113 CTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSS 172
           C QKPVIFNFGDSNSDTGG SEGLG+KFGLPTGRTFFHKP+GRLCDGRLMIDFLCESVSS
Sbjct: 38  CIQKPVIFNFGDSNSDTGGLSEGLGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVSS 97

Query: 173 DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQSRSLELIPKGYKD 232
           DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNL++QIMQFLQFQSRSLELIPKGYKD
Sbjct: 98  DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLYVQIMQFLQFQSRSLELIPKGYKD 157

Query: 233 LVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNAIWDIYEHGGRNFW 292
           LVDEEGFNNALYTIDIGQNDLAAAFTYL Y QVIQKIPSFVSEIK+AIW++Y+HGGR FW
Sbjct: 158 LVDEEGFNNALYTIDIGQNDLAAAFTYLPYSQVIQKIPSFVSEIKDAIWNLYQHGGRKFW 217

Query: 293 VDNTGPLGCLPQKLAA--TSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSALKNA 352
           + NTGPLGCLPQKLAA   +ANASD DNHGCLQSLNNAAKEFNTQL+ ACGELRSAL N 
Sbjct: 218 IHNTGPLGCLPQKLAAAYANANASDIDNHGCLQSLNNAAKEFNTQLRVACGELRSALTNV 277

Query: 353 TIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKY 412
           TIVYVD+YAIKY++IANS+SNGFENPL+VCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKY
Sbjct: 278 TIVYVDVYAIKYDIIANSVSNGFENPLIVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKY 337

Query: 413 LSWDGVHYTEAANAVFASKILSSQYSSPKLHFNFFCN 448
           +SWDGVHYTEAANAVFASKILSSQYSSPKL FNFFC+
Sbjct: 338 VSWDGVHYTEAANAVFASKILSSQYSSPKLPFNFFCS 374

BLAST of Lsi03G011400 vs. NCBI nr
Match: KAA0038109.1 (GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK20498.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 645.2 bits (1663), Expect = 4.2e-181
Identity = 305/340 (89.71%), Postives = 323/340 (95.00%), Query Frame = 0

Query: 113 CTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSS 172
           C QKPVIFNFGDSNSDTGG SEGLG+KFGLPTGRTFFHKP+GRLCDGRLMIDFLCESVSS
Sbjct: 32  CIQKPVIFNFGDSNSDTGGLSEGLGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVSS 91

Query: 173 DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQSRSLELIPKG--- 232
           DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNL++QIMQFLQFQSRSLELIPKG   
Sbjct: 92  DYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLYVQIMQFLQFQSRSLELIPKGIPR 151

Query: 233 YKDLVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNAIWDIYEHGGR 292
           YKDLVDEEGFNNALYTIDIGQNDLAAAFTYL Y QVIQKIPSFVSEIK+AIW++Y+HGGR
Sbjct: 152 YKDLVDEEGFNNALYTIDIGQNDLAAAFTYLPYSQVIQKIPSFVSEIKDAIWNLYQHGGR 211

Query: 293 NFWVDNTGPLGCLPQKLAA--TSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSAL 352
            FW+ NTGPLGCLPQKLAA   +ANASD DNHGCLQSLNNAAKEFNTQL+ ACGELRSAL
Sbjct: 212 KFWIHNTGPLGCLPQKLAAAYANANASDIDNHGCLQSLNNAAKEFNTQLRVACGELRSAL 271

Query: 353 KNATIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGFNTCNEG 412
            N TIVYVD+YAIKY++IANS+SNGFENPL+VCCGYGGPPYNFNQSVTCGQPGFNTCNEG
Sbjct: 272 TNVTIVYVDVYAIKYDIIANSVSNGFENPLIVCCGYGGPPYNFNQSVTCGQPGFNTCNEG 331

Query: 413 LKYLSWDGVHYTEAANAVFASKILSSQYSSPKLHFNFFCN 448
           LKY+SWDGVHYTEAANAVFASKILSSQYSSPKL FNFFC+
Sbjct: 332 LKYVSWDGVHYTEAANAVFASKILSSQYSSPKLPFNFFCS 371

BLAST of Lsi03G011400 vs. NCBI nr
Match: XP_023525460.1 (GDSL esterase/lipase At1g09390-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 641.3 bits (1653), Expect = 6.0e-180
Identity = 304/360 (84.44%), Postives = 321/360 (89.17%), Query Frame = 0

Query: 93  NSFPH-----FYGPSRSFSPIPMDACTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRT 152
           NSFP      F+  S     + +  C+QKPVIFNFGDSNSDTGGFSEGLGL+FG P+GR 
Sbjct: 7   NSFPFPLFVMFFVLSLPGPLLVLSQCSQKPVIFNFGDSNSDTGGFSEGLGLRFGPPSGRV 66

Query: 153 FFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGPNFTNGANFAISGSATLPKDRPFN 212
           FFHKPSGRLCDGRLMIDFLCESV SDYLTPYLRSVG NFTNG NFAISGSATLPKDRPFN
Sbjct: 67  FFHKPSGRLCDGRLMIDFLCESVGSDYLTPYLRSVGSNFTNGVNFAISGSATLPKDRPFN 126

Query: 213 LHIQIMQFLQFQSRSLELIPKGYKDLVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQ 272
           L+IQ+MQFLQFQSRSLE IPKGYKDLVDEEGFNNA+YTIDIGQNDLA +FTYLSY QVIQ
Sbjct: 127 LYIQVMQFLQFQSRSLEFIPKGYKDLVDEEGFNNAIYTIDIGQNDLAGSFTYLSYSQVIQ 186

Query: 273 KIPSFVSEIKNAIWDIYEHGGRNFWVDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNN 332
           +IPSFV+EIKNAIW IY+HGGRNFWV NTGPLGCLPQKLA  S NASD DNHGCLQSLNN
Sbjct: 187 RIPSFVTEIKNAIWTIYQHGGRNFWVHNTGPLGCLPQKLATASTNASDIDNHGCLQSLNN 246

Query: 333 AAKEFNTQLKAACGELRSALKNATIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPP 392
           AAKEFNT LKAACGELRSAL NATIVYVDIYAIKY+LIAN++ NGFENP   CCGYGGPP
Sbjct: 247 AAKEFNTLLKAACGELRSALMNATIVYVDIYAIKYDLIANAVLNGFENPSTACCGYGGPP 306

Query: 393 YNFNQSVTCGQPGFNTCNEGLKYLSWDGVHYTEAANAVFASKILSSQYSSPKLHFNFFCN 448
           YNFNQSVTCG PGFN CNEGLKY+SWDGVHYTEAAN VFASKILSSQYSSPKLHFNFFCN
Sbjct: 307 YNFNQSVTCGMPGFNVCNEGLKYVSWDGVHYTEAANGVFASKILSSQYSSPKLHFNFFCN 366

BLAST of Lsi03G011400 vs. TAIR 10
Match: AT1G09390.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 416.4 bits (1069), Expect = 2.9e-116
Identity = 204/339 (60.18%), Postives = 254/339 (74.93%), Query Frame = 0

Query: 113 CTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSS 172
           C   PVIFNFGDSNSDTGG   GLG   GLP GR+FF + +GRL DGRL+IDFLC+S+++
Sbjct: 32  CQVPPVIFNFGDSNSDTGGLVAGLGYSIGLPNGRSFFQRSTGRLSDGRLVIDFLCQSLNT 91

Query: 173 DYLTPYLRS-VGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQSRSLEL--IPKG 232
             L PYL S VG  F NGANFAI GS+TLP+  PF L+IQ+MQFL F+SR+LEL  I   
Sbjct: 92  SLLNPYLDSLVGSKFQNGANFAIVGSSTLPRYVPFALNIQLMQFLHFKSRALELASISDP 151

Query: 233 YKD-LVDEEGFNNALYTIDIGQNDLAAAFTY-LSYPQVIQKIPSFVSEIKNAIWDIYEHG 292
            K+ ++ E GF NALY IDIGQND+A +F+  LSY +V++ IP+ +SEIK+AI  +Y+ G
Sbjct: 152 LKEMMIGESGFRNALYMIDIGQNDIADSFSKGLSYSRVVKLIPNVISEIKSAIKILYDEG 211

Query: 293 GRNFWVDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSAL 352
           GR FWV NTGPLGCLPQKL  +  ++  FD HGCL + N AAK FN  L   C +LR+ L
Sbjct: 212 GRKFWVHNTGPLGCLPQKL--SMVHSKGFDKHGCLATYNAAAKLFNEGLDHMCRDLRTEL 271

Query: 353 KNATIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGFNTCNEG 412
           K A IVYVDIYAIKY+LIANS + GFE PLM CCGYGGPPYN+N ++TCG  G  +C+EG
Sbjct: 272 KEANIVYVDIYAIKYDLIANSNNYGFEKPLMACCGYGGPPYNYNVNITCGNGGSKSCDEG 331

Query: 413 LKYLSWDGVHYTEAANAVFASKILSSQYSSPKLHFNFFC 447
            +++SWDG+HYTE ANA+ A K+LS Q+S+P   F+FFC
Sbjct: 332 SRFISWDGIHYTETANAIVAMKVLSMQHSTPPTPFHFFC 368

BLAST of Lsi03G011400 vs. TAIR 10
Match: AT1G56670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 408.7 bits (1049), Expect = 6.1e-114
Identity = 200/368 (54.35%), Postives = 251/368 (68.21%), Query Frame = 0

Query: 92  SNSFPHFYGPSRSFS------PIPMDACTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTG 151
           SN+F  F+    S +      P    +CT +PVIFNFGDSNSDTGG   GLG   G P G
Sbjct: 8   SNTFSFFFITLVSLALLILRQPSRAASCTARPVIFNFGDSNSDTGGLVAGLGYPIGFPNG 67

Query: 152 RTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVG-PNFTNGANFAISGSATLPKDR 211
           R FF + +GRL DGRL+IDFLC+S+++  L PYL S+G   F NGANFAI+GS TLPK+ 
Sbjct: 68  RLFFRRSTGRLSDGRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSPTLPKNV 127

Query: 212 PFNLHIQIMQFLQFQSRSLELIPKGYK---DLVDEEGFNNALYTIDIGQNDLAAAFTY-L 271
           PF+L+IQ+ QF  F+SRSLEL           +   GF NALY IDIGQND+A +F    
Sbjct: 128 PFSLNIQVKQFSHFKSRSLELASSSNSLKGMFISNNGFKNALYMIDIGQNDIARSFARGN 187

Query: 272 SYPQVIQKIPSFVSEIKNAIWDIYEHGGRNFWVDNTGPLGCLPQKLAATSANASDFDNHG 331
           SY Q ++ IP  ++EIK++I  +Y+ GGR FW+ NTGPLGCLPQKL  +   + D D HG
Sbjct: 188 SYSQTVKLIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKL--SMVKSKDLDQHG 247

Query: 332 CLQSLNNAAKEFNTQLKAACGELRSALKNATIVYVDIYAIKYELIANSISNGFENPLMVC 391
           CL S N+AA  FN  L   C ELR+ L++ATI+Y+DIYAIKY LIANS   GF++PLM C
Sbjct: 248 CLVSYNSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQYGFKSPLMAC 307

Query: 392 CGYGGPPYNFNQSVTCGQPGFNTCNEGLKYLSWDGVHYTEAANAVFASKILSSQYSSPKL 449
           CGYGG PYN+N  +TCG  G N C EG +++SWDG+HYTE ANA+ A K+LS  YS P  
Sbjct: 308 CGYGGTPYNYNVKITCGHKGSNVCEEGSRFISWDGIHYTETANAIVAMKVLSMHYSKPPT 367

BLAST of Lsi03G011400 vs. TAIR 10
Match: AT3G62280.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 371.7 bits (953), Expect = 8.3e-103
Identity = 181/327 (55.35%), Postives = 226/327 (69.11%), Query Frame = 0

Query: 115 QKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSSDY 174
           +KP++ NFGDSNSDTGG   G+GL  GLP G TFFH+ +GRL DGRL++DF CE +   Y
Sbjct: 33  KKPILINFGDSNSDTGGVLAGVGLPIGLPHGITFFHRGTGRLGDGRLIVDFYCEHLKMTY 92

Query: 175 LTPYLRSVGPNFTNGANFAISGSATLPKDRPFNLHIQIMQFLQFQSRSLELIPKGYKDLV 234
           L+PYL S+ PNF  G NFA+SG+  LP    F L IQI QF+ F++RS ELI  G +DL+
Sbjct: 93  LSPYLDSLSPNFKRGVNFAVSGATALP-IFSFPLAIQIRQFVHFKNRSQELISSGRRDLI 152

Query: 235 DEEGFNNALYTIDIGQNDLAAAF--TYLSYPQVIQKIPSFVSEIKNAIWDIYEHGGRNFW 294
           D+ GF NALY IDIGQNDL  A   + L+Y  V++KIPS + EIK AI  +Y +GGR FW
Sbjct: 153 DDNGFRNALYMIDIGQNDLLLALYDSNLTYAPVVEKIPSMLLEIKKAIQTVYLYGGRKFW 212

Query: 295 VDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSALKNATI 354
           V NTGPLGC P++LA    N SD D  GC +  N  AK FN  L + C ELRS  K+AT+
Sbjct: 213 VHNTGPLGCAPKELAIHLHNDSDLDPIGCFRVHNEVAKAFNKGLLSLCNELRSQFKDATL 272

Query: 355 VYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKYLS 414
           VYVDIY+IKY+L A+    GF +PLM CCGYGG P N+++  TCGQPG   C +  K + 
Sbjct: 273 VYVDIYSIKYKLSADFKLYGFVDPLMACCGYGGRPNNYDRKATCGQPGSTICRDVTKAIV 332

Query: 415 WDGVHYTEAANAVFASKILSSQYSSPK 440
           WDGVHYTEAAN      +L+++YS PK
Sbjct: 333 WDGVHYTEAANRFVVDAVLTNRYSYPK 358

BLAST of Lsi03G011400 vs. TAIR 10
Match: AT1G54790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 3.9e-84
Identity = 155/339 (45.72%), Postives = 208/339 (61.36%), Query Frame = 0

Query: 117 PVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLT 176
           P  FNFGDSNSDTG    GLG++  LP G+  F   S R CDGRL+IDFL + +   +L 
Sbjct: 30  PSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQRFCDGRLVIDFLMDEMDLPFLN 89

Query: 177 PYLRSVG-PNFTNGANFAISGSATLPKD----RPFNLHIQIMQFLQFQSRSLELIPKG-- 236
           PYL S+G PNF  G NFA +GS  LP +     PF+  +QI QF++F+SR++EL+ K   
Sbjct: 90  PYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDLQISQFIRFKSRAIELLSKTGR 149

Query: 237 --YKDLVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSEIKNAIWDIYEHG 296
              K L   + ++  LY IDIGQND+A AF   +  QV+  IPS +   +  +  +YE G
Sbjct: 150 KYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVLASIPSILETFEAGLKRLYEEG 209

Query: 297 GRNFWVDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNNAAKEFNTQLKAACGELRSAL 356
           GRN W+ NTGPLGCL Q +A    +++  D  GC+ S N AAK FN QL A   + ++  
Sbjct: 210 GRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSSHNQAAKLFNLQLHAMSNKFQAQY 269

Query: 357 KNATIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVTCGQPGF------ 416
            +A + YVDI++IK  LIAN    GFE PLM CCG GG P N++  +TCGQ         
Sbjct: 270 PDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISV 329

Query: 417 --NTCNEGLKYLSWDGVHYTEAANAVFASKILSSQYSSP 439
               CN+  +Y++WDG+HYTEAAN   +S+IL+ +YS P
Sbjct: 330 TAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYSDP 368

BLAST of Lsi03G011400 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.9e-83
Identity = 155/351 (44.16%), Postives = 210/351 (59.83%), Query Frame = 0

Query: 105 FSPIPMDACTQKPVIFNFGDSNSDTGGFSEGLGLKFGLPTGRTFFHKPSGRLCDGRLMID 164
           F P       + P I NFGDSNSDTG            P G+T+F+ PSGR CDGRL++D
Sbjct: 19  FLPSSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLPSGRYCDGRLIVD 78

Query: 165 FLCESVSSDYLTPYLRSVG-PNFTNGANFAISGSATLPKD----RPFNLHIQIMQFLQFQ 224
           FL + +   +L PYL S+G PNF  G NFA +GS  LP +     PF+  +QI QF++F+
Sbjct: 79  FLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDLQISQFIRFK 138

Query: 225 SRSLELIPKG----YKDLVDEEGFNNALYTIDIGQNDLAAAFTYLSYPQVIQKIPSFVSE 284
           SR++EL+ K      K L   + ++  LY IDIGQND+A AF   +  QV+  IPS +  
Sbjct: 139 SRAIELLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVLASIPSILET 198

Query: 285 IKNAIWDIYEHGGRNFWVDNTGPLGCLPQKLAATSANASDFDNHGCLQSLNNAAKEFNTQ 344
            +  +  +YE GGRN W+ NTGPLGCL Q +A    +++  D  GC+ S N AAK FN Q
Sbjct: 199 FEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSSHNQAAKLFNLQ 258

Query: 345 LKAACGELRSALKNATIVYVDIYAIKYELIANSISNGFENPLMVCCGYGGPPYNFNQSVT 404
           L A   + ++   +A + YVDI++IK  LIAN    GFE PLM CCG GG P N++  +T
Sbjct: 259 LHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGVGGAPLNYDSRIT 318

Query: 405 CGQPGF--------NTCNEGLKYLSWDGVHYTEAANAVFASKILSSQYSSP 439
           CGQ             CN+  +Y++WDG+HYTEAAN   +S+IL+ +YS P
Sbjct: 319 CGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYSDP 369

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O805224.1e-11560.18GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2... [more]
Q9FXB68.6e-11354.35GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1[more]
Q6NLP71.2e-10155.35GDSL esterase/lipase At3g62280 OS=Arabidopsis thaliana OX=3702 GN=At3g62280 PE=2... [more]
Q3ECP62.8e-7943.01GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2... [more]
Q9FXE51.4e-7846.57Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L9E08.8e-18591.12Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G669600 PE=3 SV=1[more]
A0A1S3BHH94.8e-18390.50GDSL esterase/lipase At1g09390-like OS=Cucumis melo OX=3656 GN=LOC103489900 PE=3... [more]
A0A5A7T9T82.0e-18189.71GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237... [more]
A0A6J1KA991.9e-17983.89GDSL esterase/lipase At1g09390-like OS=Cucurbita maxima OX=3661 GN=LOC111493087 ... [more]
W9R6R25.9e-17358.17GDSL esterase/lipase OS=Morus notabilis OX=981085 GN=L484_018784 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038905016.19.9e-19194.64GDSL esterase/lipase At1g09390-like [Benincasa hispida][more]
XP_004150835.11.8e-18491.12GDSL esterase/lipase At1g09390 [Cucumis sativus] >KGN58540.1 hypothetical protei... [more]
XP_008447456.19.9e-18390.50PREDICTED: GDSL esterase/lipase At1g09390-like [Cucumis melo][more]
KAA0038109.14.2e-18189.71GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK20498.1 GDSL esterase/lipase... [more]
XP_023525460.16.0e-18084.44GDSL esterase/lipase At1g09390-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G09390.12.9e-11660.18GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G56670.16.1e-11454.35GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G62280.18.3e-10355.35GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G54790.13.9e-8445.72GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G54790.31.9e-8344.16GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 322..342
NoneNo IPR availablePANTHERPTHR22835:SF158OS01G0331100 PROTEINcoord: 110..447
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 110..447
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 116..431
NoneNo IPR availableSUPERFAMILY56399ADP-ribosylationcoord: 16..101
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 119..430
e-value: 2.1E-45
score: 155.9
IPR009297Protein of unknown function DUF952PFAMPF06108DUF952coord: 21..107
e-value: 2.3E-21
score: 75.6
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 110..436
e-value: 3.0E-64
score: 219.4
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 117..432
e-value: 7.23044E-105
score: 312.242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G011400.1Lsi03G011400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds