Lsi03G011170 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G011170
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionATP-dependent Clp protease proteolytic subunit
Locationchr03: 21354666 .. 21359327 (-)
RNA-Seq ExpressionLsi03G011170
SyntenyLsi03G011170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAGAGGTCACTGCCATCAAATTACTTGCTCGACCCATCTTCTCCTTCTCTATCTTCGCCGTCGTTGGCTTCTTCAAGCGGATAAGGCCTCTCCGACTTTGCTAATTCTCCGCACTTCCACTTCCTTCTTCCTCTATTGCTTTCTGCTGAGATTTTTCGGAACCTTTACCTGCGAAAATGGCCCATACTTTCGCCTATACATCTGCCTCGTCTTTCCGATTAAACTCCCTCGTTTCTGTACCGAATTCAAGCTCTTCTTCCGACTCCCAGAAGCTATTTCTTCCCTTGGAGCCTTTTCACTCGAGGTACTTCTCCTGGCCTGTCTTCGTTTTTTTCTGTTTCTATGCAATGTTGGTAGTATTTGGGTAGTTCGTTTGAATGTCAAAGATTGTGAATGTGTGGGCGTCGTTGGTGACACTGAAATGGATTGCCTGAATTGGTCTATTTTTTAGTTGAAGTTCGAAATTGAAAGGTTTTGATGTCCAGATGGATTTGGGTATTTGGAAATTACTAGTGCCAAGAGCACGCCCTAGGTGATTTTTGATGGTCGGTGAGATTGGGAAAATGTAGTTTTGTTTTTTAACCTCAATTCTGCTACCATGTTACACTTCTGGGGATAGACTTCAACTAAAATCCCTATCTTTGGTTCTTATTATATGTTGTATAAGTTTAGTCTTCTCTTGTTTGTCTATAAATTTTAATTGTAGCAGCATCGAGTGATGTCCTCACTATTTGCAGGAAACTCGGAAAATTAGTTGGAGGTGGAAGAAACCTCAAGAACACACCAGTCAAAGCCATCTACTCGGGCGAGTTCTCATCACTGGATAGGAATTCTCGCCAAGGAATATGGTCTATAAGGTGCATAGATTGACTAACTGGGAGTTCCCCATACGTACTTTTGGGGCCAAAGATGGCTATAATCATGATCAGTTATTGAAATGCTAAACATAGAAATGGCTTTTGAAATTATGTTCTGTATGATTCACAGGGATGATGTGCAAATTCCATCTTCACCATACTTCCCAGCTTATGCACAAGGGCAAGGTCCTCCTCCAATGGTGCAGGAACGCTTCCAAAGTGTTATAAGCCAGCTTTTTCAATATGTAAGGGTGTAGGTCTGCGATGTTTATTTCCTTGACATAGGTTTGATGTTGCACTTGTAGGTTGTTATCACCATCTTTTGATACTTTTGTGTGCTTTTGGACTAGAGAATTATTCGTTGTGGTGGAGCCGTTGATGATGATATGGCAAATATCATTGTTGCTCAACTTCTATACCTTGATGCTGTTGATCCCAATAAGGTTCTTTTTTTCTTTTTGCTCTCAACTCATTTTAAGGAAGTAGCTCATATATAGTTTATCACGTTTTCAGTATAACTAATTGATTAACATAATACTTTTGGTATGCTTAACAGGATATTGTGATGTATGTAAATTCCCCTGGTGGATCAGTTACAGCTGGTAATATTGAAAGCGGAAACGATTTCATTGATCATTTGTTTTGTTGGAAATTGGTACTGGCTTTATCTTTTCACTACTGATTAATTTGGATTTATGTAGGTATGGCCATTTTTGACACTATGAGACATATTAGACCCGATGTCTCTACTGTCTGTGTGGGACTAGCAGCCAGGTATAAGATTTATCGGTTCCATACACTTTTAAGCTGATTTGTTTTGCTTTTCTTCTTTTATTTCTTCAAGTGTCATTTACAATTAGTGTGGCATTGTTAAACTGTGCTGGGAAAAATTATTATCTAAAATAGTGGCAGATGACTTCTTCTTGGTTCCAATCATCATCTTTATCCTATAATGGACTAGTTATATGGCCTGCATAATGCGTATAATTTTGTAAAGGCTGGAGCCTTCATTATTGTTTTCCCTCCAGAGAGGCAGATTAATTAAAATGGATCGACTTCAGCTAGTCCAAATGTTTCAGCTGTAGTTTGGCCTTATATCATTAGCACACATATCTGTATTCAAAATCAATGAGATAATGCATGAACAGGTCCAAGTGGTGTTGGTAGGCTCAAAACTTGGTGTTTTAGCATGCCACCTTAATTTATTGAAGACTAAACTGGTAAAATATGTGTATGTTTGTGTGTATTCACAATGCTTCAAAGCCTGGCGCTGATGCAAGTAACATGGACTCAATTTTTCTTGTGCACTTGAATCAATAGCTTAGGAGAGATAAAATTGGGGTATCAATGACTAGGAGGAGTTTTCCTAACCTAGAGCTGTTATCTGTAACTTCTCCCTCTCATATTTTAATTAATAGTTTGTTCTTACATTGGGATTTCTGTTTGTTCTATTATTAACAGTATGGGAGCTTTCCTGCTTAGCGCTGGAACTAAAGGTACAAGTTTTTAGTGTTAACAACTTCTTGAATTTTGTTTTTGTTGTTTTCTTAAATTAAATATTTTGCTTATAAAAAATAAATGATGTATTTTGCTGTGTATTAAGTCTGCACCTTCTTGTTTATTTCTAAAGGAAAAAGATACAGTTTGCCAAATTCAAGGATAATGATCCATCAGCCTCTCGGTGGTGCTCAGGGTGGACAGACTGACATTGATATTCAGGTATCCTTATAAATCCTCTTGGATGATCAGTAATATATCAATCAAATTCGTTTTATGATTTATGTAGTTTCCTTTGATTGCATATCTATCATCAAGGTATTTCATAGTAGAGCTTTGTGGGTGAGTTTGGTAACTCATATGAAATAAGCGTTTGTTAAAAAGGAAAAACGTTTTTTTATTGAAAGTGCTTACGAAATTTGAAAAGCGTATAATAGACCACTTCACTAGTTGGTTCTAATGTTTCAAAATGGCTTTTCATCTAATCTTTTTAATTCATCTTAATGTAAAATATTAATATATACTTAAAAAATGATTTTAGAATTGACAAAGTACTGAAAAAGAAAACAACAAAACCAAAAGGAAAAAAAGTAAAGTGAGAAAGCATTTGTAGATGAAAGTCTGGGTAAATAGACAATAGTGAAGGAGTGTATAAAGAGAGATTAATTGAACAAAAATGCGTATGGAGGGAGAACATAGGGAAAAAAGTGTAGGTCAAAAGTTGTTGAGAAAAGGCATATATTTAGGAAGAAGCTAGAGAGAGGATTTCTATGGGGAGAAATTAATGATAATGTAGGTGTTAGGAAAGACTAGGTAGAGAGTGTAGAGTGAAATAAGTGAGTAAGAGTAAAGAAAATCCGGTGAGAGAAAGTATAGTTGTAGATGGAGATAAAACGAGAGAAAATGTGTTAAAGGAGAGCTAAGTTTGAGAAGAGTGCAAAGAGAGATTAGTTAGGTAAGGATGCAGTGAGAGAAAGTGTGTTTGAATTCTATGTGTTTGGGTCACCTTGCGCTACCCAACTAACCTCACAGGATAACTGCTTAATTCTACAACATTTGATTGTTAAAAAATGTGCAAGAATTTAAAACCCTTGGGTTTGAATCTCCAACCTATGGTTGTTGAGTTGTTTCACTCTGTTTAAAGCTAAGAAATTCCAACTAGCACCACTTACTCACACCTTACAAATGTCATGACGCTCAAAATTGATCATGCCTCTAACTTGTGCAACTGGAGGATTTTTGCCCAATGTTATCGACCCTCCAGGTTGATAGATTTAACAATTTTTGGCAATGTTGCAATTGATCCACACTAGCCCAGTCAAGTTTGTTGAGCTGGGTATCATTTGCTAAACCTTCAAGAAAATGAAAACTTTTATGTCCACCTTGAACAATTTTTTGGCAGGAGAATTTAGATTGCTTTTCCTTCAACAATTGTAGCAGGAATGTGTTATTGCCTTAGATTATGATTTCATCCATGTTAATGTCAAGCATTTTCAATTTAGTCCCTAAACTGTAACTTGGTTGTCAGGCAAATGAAATGTTGCATCATAAGGCAAATCTTAACGGGTATCTTTCGTACCACACCGGTCAAAGTCTTGAGAAAATTAACCAGGATACGGATCGCGATTTCTTCATGAGTGCTAAAGAAGCTAAAGAGTATGGACTCATAGATGGAGTTATAATGAATCCTCTCAAAGCTCTTCAACCTTTAGCAGCTGCCACAGAAGGCAGTGCGTAAAATTGTATCCTGCTGTGCAACCAATTAGATATTCTTCTTGTAGATTTAAAAGCGAAAACCATCGTAGTGTATGCAGATTTTGACCATTAACAAACATGGCTTCCATGCTCTCATCGTAGCTTAAGCTTGAGAGAAATCTTGAAATGGCCATTGGATTACTGTTGTTTCCTTACACCACCAAGATATTATATTGAACCGTCCATTAAAGTTACTGTCCTGATTAAAAGAAATTTCCTCTCTGGTTGGTTGGGATGACAAGAATGATACTGGCCAAACACTTGTCAATATTTGATTGGTTATTGTGAAAAGATGCCGCCGACCCTATAAAAGACTGCATTATTATGTAGTCACTTTCTTGATTGTGGATTAATATGCGTTAGGTTTCATTGAGAATTTCTAATGCATTCAGTAGCTTTCCATATACGTTGCAAGGAGGTGAACCTTGGTTGTTTCTGATATTTTAGTGTTTTATTATTCAATGGAGTTGTTACATTATGGATATGTTTCTTAAGAAATTTGGCACTGAACTAGCATTATTAGTCATTATTGATGATGTTTTCCATTTTGT

mRNA sequence

CACAGAGGTCACTGCCATCAAATTACTTGCTCGACCCATCTTCTCCTTCTCTATCTTCGCCGTCGTTGGCTTCTTCAAGCGGATAAGGCCTCTCCGACTTTGCTAATTCTCCGCACTTCCACTTCCTTCTTCCTCTATTGCTTTCTGCTGAGATTTTTCGGAACCTTTACCTGCGAAAATGGCCCATACTTTCGCCTATACATCTGCCTCGTCTTTCCGATTAAACTCCCTCGTTTCTGTACCGAATTCAAGCTCTTCTTCCGACTCCCAGAAGCTATTTCTTCCCTTGGAGCCTTTTCACTCGAGGAAACTCGGAAAATTAGTTGGAGGTGGAAGAAACCTCAAGAACACACCAGTCAAAGCCATCTACTCGGGCGAGTTCTCATCACTGGATAGGAATTCTCGCCAAGGAATATGGTCTATAAGGGATGATGTGCAAATTCCATCTTCACCATACTTCCCAGCTTATGCACAAGGGCAAGGTCCTCCTCCAATGGTGCAGGAACGCTTCCAAAGTGTTATAAGCCAGCTTTTTCAATATAGAATTATTCGTTGTGGTGGAGCCGTTGATGATGATATGGCAAATATCATTGTTGCTCAACTTCTATACCTTGATGCTGTTGATCCCAATAAGGATATTGTGATGTATGTAAATTCCCCTGGTGGATCAGTTACAGCTGGTATGGCCATTTTTGACACTATGAGACATATTAGACCCGATGTCTCTACTGTCTGTGTGGGACTAGCAGCCAGTATGGGAGCTTTCCTGCTTAGCGCTGGAACTAAAGGAAAAAGATACAGTTTGCCAAATTCAAGGATAATGATCCATCAGCCTCTCGGTGGTGCTCAGGGTGGACAGACTGACATTGATATTCAGGCAAATGAAATGTTGCATCATAAGGCAAATCTTAACGGGTATCTTTCGTACCACACCGGTCAAAGTCTTGAGAAAATTAACCAGGATACGGATCGCGATTTCTTCATGAGTGCTAAAGAAGCTAAAGAGTATGGACTCATAGATGGAGTTATAATGAATCCTCTCAAAGCTCTTCAACCTTTAGCAGCTGCCACAGAAGGCAGTGCGTAAAATTGTATCCTGCTGTGCAACCAATTAGATATTCTTCTTGTAGATTTAAAAGCGAAAACCATCGTAGTGTATGCAGATTTTGACCATTAACAAACATGGCTTCCATGCTCTCATCGTAGCTTAAGCTTGAGAGAAATCTTGAAATGGCCATTGGATTACTGTTGTTTCCTTACACCACCAAGATATTATATTGAACCGTCCATTAAAGTTACTGTCCTGATTAAAAGAAATTTCCTCTCTGGTTGGTTGGGATGACAAGAATGATACTGGCCAAACACTTGTCAATATTTGATTGGTTATTGTGAAAAGATGCCGCCGACCCTATAAAAGACTGCATTATTATGTAGTCACTTTCTTGATTGTGGATTAATATGCGTTAGGTTTCATTGAGAATTTCTAATGCATTCAGTAGCTTTCCATATACGTTGCAAGGAGGTGAACCTTGGTTGTTTCTGATATTTTAGTGTTTTATTATTCAATGGAGTTGTTACATTATGGATATGTTTCTTAAGAAATTTGGCACTGAACTAGCATTATTAGTCATTATTGATGATGTTTTCCATTTTGT

Coding sequence (CDS)

ATGGCCCATACTTTCGCCTATACATCTGCCTCGTCTTTCCGATTAAACTCCCTCGTTTCTGTACCGAATTCAAGCTCTTCTTCCGACTCCCAGAAGCTATTTCTTCCCTTGGAGCCTTTTCACTCGAGGAAACTCGGAAAATTAGTTGGAGGTGGAAGAAACCTCAAGAACACACCAGTCAAAGCCATCTACTCGGGCGAGTTCTCATCACTGGATAGGAATTCTCGCCAAGGAATATGGTCTATAAGGGATGATGTGCAAATTCCATCTTCACCATACTTCCCAGCTTATGCACAAGGGCAAGGTCCTCCTCCAATGGTGCAGGAACGCTTCCAAAGTGTTATAAGCCAGCTTTTTCAATATAGAATTATTCGTTGTGGTGGAGCCGTTGATGATGATATGGCAAATATCATTGTTGCTCAACTTCTATACCTTGATGCTGTTGATCCCAATAAGGATATTGTGATGTATGTAAATTCCCCTGGTGGATCAGTTACAGCTGGTATGGCCATTTTTGACACTATGAGACATATTAGACCCGATGTCTCTACTGTCTGTGTGGGACTAGCAGCCAGTATGGGAGCTTTCCTGCTTAGCGCTGGAACTAAAGGAAAAAGATACAGTTTGCCAAATTCAAGGATAATGATCCATCAGCCTCTCGGTGGTGCTCAGGGTGGACAGACTGACATTGATATTCAGGCAAATGAAATGTTGCATCATAAGGCAAATCTTAACGGGTATCTTTCGTACCACACCGGTCAAAGTCTTGAGAAAATTAACCAGGATACGGATCGCGATTTCTTCATGAGTGCTAAAGAAGCTAAAGAGTATGGACTCATAGATGGAGTTATAATGAATCCTCTCAAAGCTCTTCAACCTTTAGCAGCTGCCACAGAAGGCAGTGCGTAA

Protein sequence

MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTPVKAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQYRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRPDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHHKANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAATEGSA
Homology
BLAST of Lsi03G011170 vs. ExPASy Swiss-Prot
Match: Q9S834 (ATP-dependent Clp protease proteolytic subunit 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLPP5 PE=1 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 5.2e-138
Identity = 251/298 (84.23%), Postives = 263/298 (88.26%), Query Frame = 0

Query: 1   MAHTFAYTSASSFRLNS-LVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTP 60
           MAH    TSASS R  +  VS   + SS DS KL LP EP  SRK  KLV   +N KN+ 
Sbjct: 1   MAHACVSTSASSLRFTAGFVSASPNGSSFDSPKLSLPFEPLRSRKTNKLVSDRKNWKNST 60

Query: 61  VKAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLF 120
            KA+YSG   + +  S QG+WSIRDD+Q+PSSPYFPAYAQGQGPPPMVQERFQS+ISQLF
Sbjct: 61  PKAVYSGNLWTPEIPSPQGVWSIRDDLQVPSSPYFPAYAQGQGPPPMVQERFQSIISQLF 120

Query: 121 QYRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIR 180
           QYRIIRCGGAVDDDMANIIVAQLLYLDAVDP KDIVMYVNSPGGSVTAGMAIFDTMRHIR
Sbjct: 121 QYRIIRCGGAVDDDMANIIVAQLLYLDAVDPTKDIVMYVNSPGGSVTAGMAIFDTMRHIR 180

Query: 181 PDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLH 240
           PDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLH
Sbjct: 181 PDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLH 240

Query: 241 HKANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA 298
           HKANLNGYL+YHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA
Sbjct: 241 HKANLNGYLAYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA 298

BLAST of Lsi03G011170 vs. ExPASy Swiss-Prot
Match: Q3MFR5 (ATP-dependent Clp protease proteolytic subunit 1 OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=clpP1 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.4e-66
Identity = 126/191 (65.97%), Postives = 151/191 (79.06%), Query Frame = 0

Query: 109 ERFQSVISQLFQYRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAG 168
           ER   + S+L + RII  G  VD ++AN+IVAQLL+LDA DP KDI +Y+NSPGGSVTAG
Sbjct: 14  ERAFDIYSRLLRERIIFLGQQVDSNLANLIVAQLLFLDAEDPEKDIYLYINSPGGSVTAG 73

Query: 169 MAIFDTMRHIRPDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQT 228
           M IFDTM+HIRPDV T+C GLAASMGAFLLSAGTKGKR SLP+SRIMIHQPLGGAQG  T
Sbjct: 74  MGIFDTMKHIRPDVCTICTGLAASMGAFLLSAGTKGKRMSLPHSRIMIHQPLGGAQGQAT 133

Query: 229 DIDIQANEMLHHKANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPL 288
           DI+IQA E+L+HK  LN YL+ HTGQ +E+I +DT+RDFFMS  EA++YGLID VI    
Sbjct: 134 DIEIQAREILYHKRRLNDYLAEHTGQPIERIAEDTERDFFMSPDEARDYGLIDQVIDRHA 193

Query: 289 KALQPLAAATE 300
              +P+A   +
Sbjct: 194 AGSRPVAMVNQ 204

BLAST of Lsi03G011170 vs. ExPASy Swiss-Prot
Match: Q8YXH5 (ATP-dependent Clp protease proteolytic subunit 1 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=clpP1 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.1e-66
Identity = 125/187 (66.84%), Postives = 149/187 (79.68%), Query Frame = 0

Query: 109 ERFQSVISQLFQYRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAG 168
           ER   + S+L + RI+  G  VD ++AN+IVAQLL+LDA DP KDI +Y+NSPGGSVTAG
Sbjct: 14  ERAFDIYSRLLRERIVFLGQQVDSNLANLIVAQLLFLDAEDPEKDIYLYINSPGGSVTAG 73

Query: 169 MAIFDTMRHIRPDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQT 228
           M IFDTM+HIRPDV T+C GLAASMGAFLLSAG KGKR SLP+SRIMIHQPLGGAQG  T
Sbjct: 74  MGIFDTMKHIRPDVCTICTGLAASMGAFLLSAGAKGKRMSLPHSRIMIHQPLGGAQGQAT 133

Query: 229 DIDIQANEMLHHKANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPL 288
           DI+IQA E+L+HK  LN YL+ HTGQ +E+I +DT+RDFFMS  EAK+YGLID VI    
Sbjct: 134 DIEIQAREILYHKRRLNDYLAEHTGQPIERIAEDTERDFFMSPDEAKDYGLIDQVIDRHA 193

Query: 289 KALQPLA 296
              +P+A
Sbjct: 194 AGSRPVA 200

BLAST of Lsi03G011170 vs. ExPASy Swiss-Prot
Match: Q2JIP1 (ATP-dependent Clp protease proteolytic subunit 2 OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=clpP2 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.4e-63
Identity = 124/189 (65.61%), Postives = 152/189 (80.42%), Query Frame = 0

Query: 109 ERFQSVISQLFQYRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAG 168
           ER   + S+L + RII  G  VDDD+AN+IVAQ+LYL++ DP KDI +Y+NSPGGSV AG
Sbjct: 13  ERAFDIYSRLLRDRIIFLGTQVDDDIANLIVAQMLYLESEDPEKDIYLYINSPGGSVYAG 72

Query: 169 MAIFDTMRHIRPDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQT 228
           MAI+DTM+HI+PDVST+C+GLAASMGAFLL+ GTKGKR +LP++RIMIHQPLGGAQG  T
Sbjct: 73  MAIYDTMQHIQPDVSTICIGLAASMGAFLLAGGTKGKRIALPHARIMIHQPLGGAQGPAT 132

Query: 229 DIDIQANEMLHHKANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPL 288
           DI+IQA E+L  K +LN  L+YHTGQ LE+I +DTDRD FM+ ++AKEYGLID VI    
Sbjct: 133 DIEIQAKEILFIKNSLNSLLAYHTGQPLERIERDTDRDNFMTPEQAKEYGLIDQVIS--- 192

Query: 289 KALQPLAAA 298
           K  QP  AA
Sbjct: 193 KRPQPTLAA 198

BLAST of Lsi03G011170 vs. ExPASy Swiss-Prot
Match: P54416 (ATP-dependent Clp protease proteolytic subunit 1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=clpP1 PE=3 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 9.4e-63
Identity = 121/186 (65.05%), Postives = 148/186 (79.57%), Query Frame = 0

Query: 109 ERFQSVISQLFQYRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAG 168
           +R   + S+L + RI+  G  V D+ AN++VAQLL+L+A DP KDI +Y+NSPGGSV+AG
Sbjct: 13  DRAFDIYSRLLRERIVFLGQEVRDENANLVVAQLLFLEAEDPEKDIYLYINSPGGSVSAG 72

Query: 169 MAIFDTMRHIRPDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQT 228
           + IFDTM  IRPDV T+C+GLAASMGAFLLSAG KGKR SLPNSRIMIHQPLGGAQG  T
Sbjct: 73  LGIFDTMNQIRPDVCTICIGLAASMGAFLLSAGAKGKRMSLPNSRIMIHQPLGGAQGQAT 132

Query: 229 DIDIQANEMLHHKANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPL 288
           DI+IQA E+L+ KA LN +L+ HTG+SLE+I  DT+RDFFMSA+E+KEYGLID VI    
Sbjct: 133 DIEIQAKEILYLKALLNQHLANHTGKSLEEITADTERDFFMSAEESKEYGLIDQVINRRP 192

Query: 289 KALQPL 295
            A  P+
Sbjct: 193 SASDPI 198

BLAST of Lsi03G011170 vs. ExPASy TrEMBL
Match: A0A5A7TNH0 (ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001490 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 290/297 (97.64%), Postives = 295/297 (99.33%), Query Frame = 0

Query: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTPV 60
           MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQK FLPLEPFHSRKLGKLVGGGRNLKN+PV
Sbjct: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKFFLPLEPFHSRKLGKLVGGGRNLKNSPV 60

Query: 61  KAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQ 120
           KA+YSGEFSS+DRNSRQGIWSIRDDVQ+PSSPYFP YAQGQGPPPMVQERFQSVISQLFQ
Sbjct: 61  KAMYSGEFSSMDRNSRQGIWSIRDDVQVPSSPYFPVYAQGQGPPPMVQERFQSVISQLFQ 120

Query: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180
           YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP
Sbjct: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180

Query: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240
           DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH
Sbjct: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240

Query: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA 298
           KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAK+YGLIDGVIMNPLKALQPLAAA
Sbjct: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKDYGLIDGVIMNPLKALQPLAAA 297

BLAST of Lsi03G011170 vs. ExPASy TrEMBL
Match: A0A1S3BY88 (ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo OX=3656 GN=LOC103494727 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 290/297 (97.64%), Postives = 295/297 (99.33%), Query Frame = 0

Query: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTPV 60
           MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQK FLPLEPFHSRKLGKLVGGGRNLKN+PV
Sbjct: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKFFLPLEPFHSRKLGKLVGGGRNLKNSPV 60

Query: 61  KAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQ 120
           KA+YSGEFSS+DRNSRQGIWSIRDDVQ+PSSPYFP YAQGQGPPPMVQERFQSVISQLFQ
Sbjct: 61  KAMYSGEFSSMDRNSRQGIWSIRDDVQVPSSPYFPVYAQGQGPPPMVQERFQSVISQLFQ 120

Query: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180
           YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP
Sbjct: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180

Query: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240
           DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH
Sbjct: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240

Query: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA 298
           KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAK+YGLIDGVIMNPLKALQPLAAA
Sbjct: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKDYGLIDGVIMNPLKALQPLAAA 297

BLAST of Lsi03G011170 vs. ExPASy TrEMBL
Match: A0A6J1DRD0 (ATP-dependent Clp protease proteolytic subunit OS=Momordica charantia OX=3673 GN=LOC111022448 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 4.5e-161
Identity = 290/302 (96.03%), Postives = 294/302 (97.35%), Query Frame = 0

Query: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTPV 60
           MAHTFAYTSASSFRLNSLVSVPNSSSSS+SQKLFLPL+PF SRKLGKLVGGGRNLKNTPV
Sbjct: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSESQKLFLPLDPFQSRKLGKLVGGGRNLKNTPV 60

Query: 61  KAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQ 120
           KAIYS EFSSLDR+SRQG+WSIRDDVQIPSSPYFP YAQGQGPPPMVQERF SVISQLFQ
Sbjct: 61  KAIYSSEFSSLDRSSRQGLWSIRDDVQIPSSPYFPTYAQGQGPPPMVQERFHSVISQLFQ 120

Query: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180
           YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP
Sbjct: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180

Query: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240
           DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH
Sbjct: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240

Query: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAATEG 300
           KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA   
Sbjct: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAAAAT 300

Query: 301 SA 303
            A
Sbjct: 301 EA 302

BLAST of Lsi03G011170 vs. ExPASy TrEMBL
Match: A0A6J1K7I3 (ATP-dependent Clp protease proteolytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111492965 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.1e-158
Identity = 282/297 (94.95%), Postives = 293/297 (98.65%), Query Frame = 0

Query: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTPV 60
           MAHTF YTSASSFRLNSLVSVPNSSSSSDSQKLFLPLE FHSRKLGKLVGGGRNLKN+PV
Sbjct: 1   MAHTFTYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLENFHSRKLGKLVGGGRNLKNSPV 60

Query: 61  KAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQ 120
           KAIYSGEFSSLD++SRQG+WSIRDDVQIPSSPYFP YAQGQGPPPMVQERFQSVISQLFQ
Sbjct: 61  KAIYSGEFSSLDKSSRQGLWSIRDDVQIPSSPYFPTYAQGQGPPPMVQERFQSVISQLFQ 120

Query: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180
           +RI+RCGGAVDDDMANIIVAQLLYLDAVDPNKDI++YVNSPGGSVTAGMAIFDTMRHIRP
Sbjct: 121 HRIVRCGGAVDDDMANIIVAQLLYLDAVDPNKDIILYVNSPGGSVTAGMAIFDTMRHIRP 180

Query: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240
           DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEML+H
Sbjct: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLYH 240

Query: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA 298
           KANLNGYLSYHTGQSLEKIN+DTDRDFFMSAKEA+EYGLIDGVIMNPLKA QPLAAA
Sbjct: 241 KANLNGYLSYHTGQSLEKINEDTDRDFFMSAKEAEEYGLIDGVIMNPLKAFQPLAAA 297

BLAST of Lsi03G011170 vs. ExPASy TrEMBL
Match: A0A6J1GAD0 (ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111452367 PE=3 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.2e-157
Identity = 279/297 (93.94%), Postives = 292/297 (98.32%), Query Frame = 0

Query: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTPV 60
           MAHTF YTSASSFRLNSLVSVPNSSSSS+SQKLFLPLE FHSRKLGK VGGGRNLKN+PV
Sbjct: 1   MAHTFTYTSASSFRLNSLVSVPNSSSSSESQKLFLPLENFHSRKLGKFVGGGRNLKNSPV 60

Query: 61  KAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQ 120
           KAIYSGEFSSLD++SR+G+WSIRDDVQIPSSPYFP YAQGQGPPPMVQERFQSVISQLFQ
Sbjct: 61  KAIYSGEFSSLDKSSRRGLWSIRDDVQIPSSPYFPTYAQGQGPPPMVQERFQSVISQLFQ 120

Query: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180
           +RI+RCGGAVDDDMANIIVAQLLYLDAVDPNKDI++YVNSPGGSVTAGMAIFDTMRHIRP
Sbjct: 121 HRIVRCGGAVDDDMANIIVAQLLYLDAVDPNKDIILYVNSPGGSVTAGMAIFDTMRHIRP 180

Query: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240
           DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEML+H
Sbjct: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLYH 240

Query: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA 298
           KANLNGYLSYHTGQSLEKIN+DTDRDFFMSAKEA+EYGLIDGVIMNPLKA QPLAAA
Sbjct: 241 KANLNGYLSYHTGQSLEKINEDTDRDFFMSAKEAEEYGLIDGVIMNPLKAFQPLAAA 297

BLAST of Lsi03G011170 vs. NCBI nr
Match: XP_038906134.1 (ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Benincasa hispida])

HSP 1 Score: 583.9 bits (1504), Expect = 7.7e-163
Identity = 292/299 (97.66%), Postives = 296/299 (99.00%), Query Frame = 0

Query: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTPV 60
           MAHTFAYTSASSFRLNSLVSVPNSSSSS+S KLFLPLEPFHSRKLGKLVGGGRNLKN+PV
Sbjct: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSESHKLFLPLEPFHSRKLGKLVGGGRNLKNSPV 60

Query: 61  KAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQ 120
           K++YSGE SSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQ
Sbjct: 61  KSVYSGELSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQ 120

Query: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180
           YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP
Sbjct: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180

Query: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240
           DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH
Sbjct: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240

Query: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAATE 300
           KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA E
Sbjct: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAAAE 299

BLAST of Lsi03G011170 vs. NCBI nr
Match: XP_008454281.1 (PREDICTED: ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Cucumis melo] >KAA0044418.1 ATP-dependent Clp protease proteolytic subunit 5 [Cucumis melo var. makuwa] >TYK29545.1 ATP-dependent Clp protease proteolytic subunit 5 [Cucumis melo var. makuwa])

HSP 1 Score: 582.4 bits (1500), Expect = 2.2e-162
Identity = 290/297 (97.64%), Postives = 295/297 (99.33%), Query Frame = 0

Query: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTPV 60
           MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQK FLPLEPFHSRKLGKLVGGGRNLKN+PV
Sbjct: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKFFLPLEPFHSRKLGKLVGGGRNLKNSPV 60

Query: 61  KAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQ 120
           KA+YSGEFSS+DRNSRQGIWSIRDDVQ+PSSPYFP YAQGQGPPPMVQERFQSVISQLFQ
Sbjct: 61  KAMYSGEFSSMDRNSRQGIWSIRDDVQVPSSPYFPVYAQGQGPPPMVQERFQSVISQLFQ 120

Query: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180
           YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP
Sbjct: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180

Query: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240
           DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH
Sbjct: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240

Query: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA 298
           KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAK+YGLIDGVIMNPLKALQPLAAA
Sbjct: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKDYGLIDGVIMNPLKALQPLAAA 297

BLAST of Lsi03G011170 vs. NCBI nr
Match: XP_022155316.1 (ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Momordica charantia])

HSP 1 Score: 577.0 bits (1486), Expect = 9.4e-161
Identity = 290/302 (96.03%), Postives = 294/302 (97.35%), Query Frame = 0

Query: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTPV 60
           MAHTFAYTSASSFRLNSLVSVPNSSSSS+SQKLFLPL+PF SRKLGKLVGGGRNLKNTPV
Sbjct: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSESQKLFLPLDPFQSRKLGKLVGGGRNLKNTPV 60

Query: 61  KAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQ 120
           KAIYS EFSSLDR+SRQG+WSIRDDVQIPSSPYFP YAQGQGPPPMVQERF SVISQLFQ
Sbjct: 61  KAIYSSEFSSLDRSSRQGLWSIRDDVQIPSSPYFPTYAQGQGPPPMVQERFHSVISQLFQ 120

Query: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180
           YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP
Sbjct: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180

Query: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240
           DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH
Sbjct: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240

Query: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAATEG 300
           KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA   
Sbjct: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAAAAT 300

Query: 301 SA 303
            A
Sbjct: 301 EA 302

BLAST of Lsi03G011170 vs. NCBI nr
Match: XP_022998282.1 (ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Cucurbita maxima])

HSP 1 Score: 568.2 bits (1463), Expect = 4.4e-158
Identity = 282/297 (94.95%), Postives = 293/297 (98.65%), Query Frame = 0

Query: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTPV 60
           MAHTF YTSASSFRLNSLVSVPNSSSSSDSQKLFLPLE FHSRKLGKLVGGGRNLKN+PV
Sbjct: 1   MAHTFTYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLENFHSRKLGKLVGGGRNLKNSPV 60

Query: 61  KAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQ 120
           KAIYSGEFSSLD++SRQG+WSIRDDVQIPSSPYFP YAQGQGPPPMVQERFQSVISQLFQ
Sbjct: 61  KAIYSGEFSSLDKSSRQGLWSIRDDVQIPSSPYFPTYAQGQGPPPMVQERFQSVISQLFQ 120

Query: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180
           +RI+RCGGAVDDDMANIIVAQLLYLDAVDPNKDI++YVNSPGGSVTAGMAIFDTMRHIRP
Sbjct: 121 HRIVRCGGAVDDDMANIIVAQLLYLDAVDPNKDIILYVNSPGGSVTAGMAIFDTMRHIRP 180

Query: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240
           DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEML+H
Sbjct: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLYH 240

Query: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA 298
           KANLNGYLSYHTGQSLEKIN+DTDRDFFMSAKEA+EYGLIDGVIMNPLKA QPLAAA
Sbjct: 241 KANLNGYLSYHTGQSLEKINEDTDRDFFMSAKEAEEYGLIDGVIMNPLKAFQPLAAA 297

BLAST of Lsi03G011170 vs. NCBI nr
Match: KAG7036637.1 (ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 566.6 bits (1459), Expect = 1.3e-157
Identity = 281/297 (94.61%), Postives = 293/297 (98.65%), Query Frame = 0

Query: 1   MAHTFAYTSASSFRLNSLVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTPV 60
           MAHTF YTSASSFRLNSLVSVPNSSSSS+SQKLFLPLE FHSRKLGKLVGGGRNLKN+PV
Sbjct: 1   MAHTFTYTSASSFRLNSLVSVPNSSSSSESQKLFLPLENFHSRKLGKLVGGGRNLKNSPV 60

Query: 61  KAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQ 120
           KAIYSGEFSSLD++SRQG+WSIRDDVQIPSSPYFP YAQGQGPPPMVQERFQSVISQLFQ
Sbjct: 61  KAIYSGEFSSLDKSSRQGLWSIRDDVQIPSSPYFPTYAQGQGPPPMVQERFQSVISQLFQ 120

Query: 121 YRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRP 180
           +RI+RCGGAVDDDMANIIVAQLLYLDAVDPNKDI++YVNSPGGSVTAGMAIFDTMRHIRP
Sbjct: 121 HRIVRCGGAVDDDMANIIVAQLLYLDAVDPNKDIILYVNSPGGSVTAGMAIFDTMRHIRP 180

Query: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHH 240
           DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEML+H
Sbjct: 181 DVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLYH 240

Query: 241 KANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA 298
           KANLNGYLSYHTGQSLEKIN+DTDRDFFMSAKEA+EYGLIDGVIMNPLKA QPLAAA
Sbjct: 241 KANLNGYLSYHTGQSLEKINEDTDRDFFMSAKEAEEYGLIDGVIMNPLKAFQPLAAA 297

BLAST of Lsi03G011170 vs. TAIR 10
Match: AT1G02560.1 (nuclear encoded CLP protease 5 )

HSP 1 Score: 491.9 bits (1265), Expect = 3.7e-139
Identity = 251/298 (84.23%), Postives = 263/298 (88.26%), Query Frame = 0

Query: 1   MAHTFAYTSASSFRLNS-LVSVPNSSSSSDSQKLFLPLEPFHSRKLGKLVGGGRNLKNTP 60
           MAH    TSASS R  +  VS   + SS DS KL LP EP  SRK  KLV   +N KN+ 
Sbjct: 1   MAHACVSTSASSLRFTAGFVSASPNGSSFDSPKLSLPFEPLRSRKTNKLVSDRKNWKNST 60

Query: 61  VKAIYSGEFSSLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLF 120
            KA+YSG   + +  S QG+WSIRDD+Q+PSSPYFPAYAQGQGPPPMVQERFQS+ISQLF
Sbjct: 61  PKAVYSGNLWTPEIPSPQGVWSIRDDLQVPSSPYFPAYAQGQGPPPMVQERFQSIISQLF 120

Query: 121 QYRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIR 180
           QYRIIRCGGAVDDDMANIIVAQLLYLDAVDP KDIVMYVNSPGGSVTAGMAIFDTMRHIR
Sbjct: 121 QYRIIRCGGAVDDDMANIIVAQLLYLDAVDPTKDIVMYVNSPGGSVTAGMAIFDTMRHIR 180

Query: 181 PDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLH 240
           PDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLH
Sbjct: 181 PDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLH 240

Query: 241 HKANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA 298
           HKANLNGYL+YHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA
Sbjct: 241 HKANLNGYLAYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVIMNPLKALQPLAAA 298

BLAST of Lsi03G011170 vs. TAIR 10
Match: AT1G66670.1 (CLP protease proteolytic subunit 3 )

HSP 1 Score: 196.8 bits (499), Expect = 2.5e-50
Identity = 106/215 (49.30%), Postives = 142/215 (66.05%), Query Frame = 0

Query: 70  SLDRNSRQGIWSIRDDVQIPSSPYFPAYAQGQGPPPMVQERFQSVISQLFQYRIIRCGGA 129
           SL +  RQ + S  D     SS    + AQ     P  +E      + L + RI+  G  
Sbjct: 48  SLSKPPRQTLSSNWD----VSSFSIDSVAQSPSRLPSFEE--LDTTNMLLRQRIVFLGSQ 107

Query: 130 VDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRPDVSTVCVGL 189
           VDD  A+++++QLL LDA D  +DI +++NSPGGS+TAGM I+D M+  + DVSTVC+GL
Sbjct: 108 VDDMTADLVISQLLLLDAEDSERDITLFINSPGGSITAGMGIYDAMKQCKADVSTVCLGL 167

Query: 190 AASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHHKANLNGYLS 249
           AASMGAFLL++G+KGKRY +PNS++MIHQPLG A G  T++ I+  EM++HK  LN   S
Sbjct: 168 AASMGAFLLASGSKGKRYCMPNSKVMIHQPLGTAGGKATEMSIRIREMMYHKIKLNKIFS 227

Query: 250 YHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVI 285
             TG+   +I  DTDRD F++  EAKEYGLID VI
Sbjct: 228 RITGKPESEIESDTDRDNFLNPWEAKEYGLIDAVI 256

BLAST of Lsi03G011170 vs. TAIR 10
Match: AT5G45390.1 (CLP protease P4 )

HSP 1 Score: 189.1 bits (479), Expect = 5.1e-48
Identity = 90/171 (52.63%), Postives = 130/171 (76.02%), Query Frame = 0

Query: 114 VISQLFQYRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAGMAIFD 173
           V+  L + RI+  G ++DD +A+ I++QLL LDA DP KDI +++NSPGGS++A MAI+D
Sbjct: 80  VMGLLLRERIVFLGSSIDDFVADAIMSQLLLLDAKDPKKDIKLFINSPGGSLSATMAIYD 139

Query: 174 TMRHIRPDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQ 233
            ++ +R DVST+ +G+AAS  + +L AGTKGKR+++PN+RIMIHQPLGGA G   D++IQ
Sbjct: 140 VVQLVRADVSTIALGIAASTASIILGAGTKGKRFAMPNTRIMIHQPLGGASGQAIDVEIQ 199

Query: 234 ANEMLHHKANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVI 285
           A E++H+K N+   ++  T +S E++ +D DRD +MS  EA EYGLIDGVI
Sbjct: 200 AKEVMHNKNNVTSIIAGCTSRSFEQVLKDIDRDRYMSPIEAVEYGLIDGVI 250

BLAST of Lsi03G011170 vs. TAIR 10
Match: AT5G23140.1 (nuclear-encoded CLP protease P7 )

HSP 1 Score: 179.5 bits (454), Expect = 4.1e-45
Identity = 88/176 (50.00%), Postives = 124/176 (70.45%), Query Frame = 0

Query: 109 ERFQSVISQLFQYRIIRCGGAVDDDMANIIVAQLLYLDAVDPNKDIVMYVNSPGGSVTAG 168
           ER   + S+L + RII   G ++DD ++++VAQLLYL++ +P+K I MY+NSPGG VTAG
Sbjct: 44  ERAYDIFSRLLKERIICINGPINDDTSHVVVAQLLYLESENPSKPIHMYLNSPGGHVTAG 103

Query: 169 MAIFDTMRHIRPDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQT 228
           +AI+DTM++IR  +ST+C+G AASM + LL+AG KG+R SLPN+ +MIHQP GG  G   
Sbjct: 104 LAIYDTMQYIRSPISTICLGQAASMASLLLAAGAKGQRRSLPNATVMIHQPSGGYSGQAK 163

Query: 229 DIDIQANEMLHHKANLNGYLSYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVI 285
           DI I   +++     LN     HTGQ L+ +  + DRD FM+ +EAK +G+ID VI
Sbjct: 164 DITIHTKQIVRVWDALNELYVKHTGQPLDVVANNMDRDHFMTPEEAKAFGIIDEVI 219

BLAST of Lsi03G011170 vs. TAIR 10
Match: AT1G11750.1 (CLP protease proteolytic subunit 6 )

HSP 1 Score: 157.5 bits (397), Expect = 1.7e-38
Identity = 83/199 (41.71%), Postives = 122/199 (61.31%), Query Frame = 0

Query: 91  SPYFPAYAQGQGPPPMVQERFQ-----SVISQLFQYRIIRCGGAVDDDMANIIVAQLLYL 150
           +P F      +G PP++           + S LF+ RII  G  ++  +A  +++QL+ L
Sbjct: 69  APRFGVIEAKKGNPPVMPSVMTPGGPLDLSSVLFRNRIIFIGQPINAQVAQRVISQLVTL 128

Query: 151 DAVDPNKDIVMYVNSPGGSVTAGMAIFDTMRHIRPDVSTVCVGLAASMGAFLLSAGTKGK 210
            ++D   DI+MY+N PGGS  + +AI+D M  I+P V TV  G+AAS GA LL+ G KG 
Sbjct: 129 ASIDDKSDILMYLNCPGGSTYSVLAIYDCMSWIKPKVGTVAFGVAASQGALLLAGGEKGM 188

Query: 211 RYSLPNSRIMIHQPLGGAQGGQTDIDIQANEMLHHKANLNGYLSYHTGQSLEKINQDTDR 270
           RY++PN+R+MIHQP  G  G   D+  Q NE +  +  ++   +  TGQ LEK+ Q T+R
Sbjct: 189 RYAMPNTRVMIHQPQTGCGGHVEDVRRQVNEAIEARQKIDRMYAAFTGQPLEKVQQYTER 248

Query: 271 DFFMSAKEAKEYGLIDGVI 285
           D F+SA EA E+GLIDG++
Sbjct: 249 DRFLSASEALEFGLIDGLL 267

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9S8345.2e-13884.23ATP-dependent Clp protease proteolytic subunit 5, chloroplastic OS=Arabidopsis t... [more]
Q3MFR51.4e-6665.97ATP-dependent Clp protease proteolytic subunit 1 OS=Trichormus variabilis (strai... [more]
Q8YXH54.1e-6666.84ATP-dependent Clp protease proteolytic subunit 1 OS=Nostoc sp. (strain PCC 7120 ... [more]
Q2JIP11.4e-6365.61ATP-dependent Clp protease proteolytic subunit 2 OS=Synechococcus sp. (strain JA... [more]
P544169.4e-6365.05ATP-dependent Clp protease proteolytic subunit 1 OS=Synechocystis sp. (strain PC... [more]
Match NameE-valueIdentityDescription
A0A5A7TNH01.1e-16297.64ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BY881.1e-16297.64ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1DRD04.5e-16196.03ATP-dependent Clp protease proteolytic subunit OS=Momordica charantia OX=3673 GN... [more]
A0A6J1K7I32.1e-15894.95ATP-dependent Clp protease proteolytic subunit OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GAD05.2e-15793.94ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
XP_038906134.17.7e-16397.66ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Benincasa hispi... [more]
XP_008454281.12.2e-16297.64PREDICTED: ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Cucu... [more]
XP_022155316.19.4e-16196.03ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Momordica chara... [more]
XP_022998282.14.4e-15894.95ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Cucurbita maxim... [more]
KAG7036637.11.3e-15794.61ATP-dependent Clp protease proteolytic subunit 5, chloroplastic [Cucurbita argyr... [more]
Match NameE-valueIdentityDescription
AT1G02560.13.7e-13984.23nuclear encoded CLP protease 5 [more]
AT1G66670.12.5e-5049.30CLP protease proteolytic subunit 3 [more]
AT5G45390.15.1e-4852.63CLP protease P4 [more]
AT5G23140.14.1e-4550.00nuclear-encoded CLP protease P7 [more]
AT1G11750.11.7e-3841.71CLP protease proteolytic subunit 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001907ATP-dependent Clp protease proteolytic subunitPRINTSPR00127CLPPROTEASEPcoord: 153..173
score: 61.19
coord: 205..224
score: 64.62
coord: 184..201
score: 66.67
coord: 262..281
score: 66.88
coord: 113..128
score: 29.84
IPR001907ATP-dependent Clp protease proteolytic subunitHAMAPMF_00444ClpPcoord: 90..287
score: 39.330757
IPR001907ATP-dependent Clp protease proteolytic subunitCDDcd07017S14_ClpP_2coord: 114..283
e-value: 3.31216E-107
score: 307.061
NoneNo IPR availableGENE3D3.90.226.10coord: 105..287
e-value: 3.9E-82
score: 276.7
NoneNo IPR availablePANTHERPTHR10381:SF12ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT 5, CHLOROPLASTICcoord: 33..299
IPR023562Clp protease proteolytic subunit /Translocation-enhancing protein TepAPFAMPF00574CLP_proteasecoord: 108..286
e-value: 2.7E-80
score: 268.6
IPR023562Clp protease proteolytic subunit /Translocation-enhancing protein TepAPANTHERPTHR10381ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNITcoord: 33..299
IPR033135ClpP, histidine active sitePROSITEPS00382CLP_PROTEASE_HIScoord: 206..219
IPR018215ClpP, Ser active sitePROSITEPS00381CLP_PROTEASE_SERcoord: 184..195
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 109..284

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G011170.1Lsi03G011170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006515 protein quality control for misfolded or incompletely synthesized proteins
biological_process GO:0006508 proteolysis
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0009368 endopeptidase Clp complex
cellular_component GO:0009526 plastid envelope
molecular_function GO:0051117 ATPase binding
molecular_function GO:0004176 ATP-dependent peptidase activity
molecular_function GO:0004252 serine-type endopeptidase activity