Lsi03G010830 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G010830
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionEpoxide hydrolase 2
Locationchr03: 20700990 .. 20702347 (-)
RNA-Seq ExpressionLsi03G010830
SyntenyLsi03G010830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAAATCCAGCACTCAACCATCGCCACCAATGGCATTAACATCCACGTCGCTTCCATTGGCTCTGGTCCGCCAGTGCTATTCCTCCACGGTTTCCCGGAGATTTGGTACACATGGCGCCACCAGCTCCTCTTTCTCGCTTCCAAGGGCTTCCGAGCCATAGCACCTGACCTCCGTGGCTTCGGCGACTCCGATGCGCCACCGTCTCCCTCCTCCTATACGCCTCTCTACATCATTGGCGACCTTATTGGCCTCCTCGATCATCTCGAAATCGATAAAGTTTTCTTGGTAGGCCACGATTGGGGCGCTATGATGGCTTGGTACTTTTGCCTTTTTAGGCCATATCGAGTCAAGGCTTTGGTCAACTTGAGTATCAATTACTTTCCTCGGAATCCGATAATGTCGCTTCTGACCACTGCCCGCATGTTCCTCGGCGATGATTTCTACATGTGTAAATTTCAGGTGAAGGCGATTGAACCTTTGAACTCTTCTTTAATCTCTGAAATTGAATGAATCGGTCGCATTAGGTCAACATACGAATCGCTATAATCGTTGAAATTGAATGAGTTGAATCGCATTAAATGAAGTGATGAATCGGAAATTGTGAATTTCGGAACAGGAACCTGGAGAGGCGGAAGCCGATTTTGGGTCTGCAGATACGGCTACGATGATAAACTCCTGACAATGAGAGATCCAAGCCCTCCGATTATACCTAACGGAATCAAAGTACTAGCAACTCCAGAAACCTTACCTCCATGGTTAACAGAGGAAGATATCGATTATTACGCCTCCAAATTCGCCAAAACTGGCTTCACTGGCGGATTTAACTACTATCGCGCCATTGACCTGTGAGAATTCACAAATTCAATTTCTCTGTTGTGTTATGGCTTATTAAGGAACCAATTTTTGTGTGTTTTGTTGATTCATTGCCGAAGTTGGGAGCTCACAGGGCCATGGACTGGAGCACAGATCAAAGTTCCTACGAAATTCATAGTGGGGGATTTGGATTTGGCGTACCATTTTCCAGGTGTGAACGAGTATATTCATGATGGCGAATTCAAGAACGACGTACCGCTTCTGGAGGAAGTGGTAGTGATAAAAGGAGCAGCTCACTACATTAACTAAGAAAAAGCAGACGAAATAAGCTCCCTCATCTATGATTTCATCAACAAATTCTGATTTCTGAATAATCCTCTGCTTATCCTTTCCATGGCGGCAGTGCAATGACAAACAACAACCTCTCGAATGTCATATCATATCATATCGTTATTGTTACTATTATTATTTCCTTTCTTTCTTTATATTTCTACTTTGGGATATTGCGAATAACTAATATATGAAATAATAAATATTCC

mRNA sequence

ATGGAGAAAATCCAGCACTCAACCATCGCCACCAATGGCATTAACATCCACGTCGCTTCCATTGGCTCTGGTCCGCCAGTGCTATTCCTCCACGGTTTCCCGGAGATTTGGTACACATGGCGCCACCAGCTCCTCTTTCTCGCTTCCAAGGGCTTCCGAGCCATAGCACCTGACCTCCGTGGCTTCGGCGACTCCGATGCGCCACCGTCTCCCTCCTCCTATACGCCTCTCTACATCATTGGCGACCTTATTGGCCTCCTCGATCATCTCGAAATCGATAAAGTTTTCTTGGTAGGCCACGATTGGGGCGCTATGATGGCTTGGTACTTTTGCCTTTTTAGGCCATATCGAGTCAAGGCTTTGGTCAACTTGAGTATCAATTACTTTCCTCGGAATCCGATAATGTCGCTTCTGACCACTGCCCGCATGTTCCTCGGCGATGATTTCTACATTGATGAATCGGAAATTGTGAATTTCGGAACAGGAACCTGGAGAGGCGGAAGCCGATTTTGGGTCTGCAGATACGGCTACGATGATAAACTCCTGACAATGAGAGATCCAAGCCCTCCGATTATACCTAACGGAATCAAAGTACTAGCAACTCCAGAAACCTTACCTCCATGGTTAACAGAGGAAGATATCGATTATTACGCCTCCAAATTCGCCAAAACTGGCTTCACTGGCGGATTTAACTACTATCGCGCCATTGACCTTTGGGAGCTCACAGGGCCATGGACTGGAGCACAGATCAAAGTTCCTACGAAATTCATAGTGGGGGATTTGGATTTGGCGTACCATTTTCCAGGTGTGAACGAGTATATTCATGATGGCGAATTCAAGAACGACGTACCGCTTCTGGAGGAAGTGGTAGTGATAAAAGGAGCAGCTCACTACATTAACTAAGAAAAAGCAGACGAAATAAGCTCCCTCATCTATGATTTCATCAACAAATTCTGATTTCTGAATAATCCTCTGCTTATCCTTTCCATGGCGGCAGTGCAATGACAAACAACAACCTCTCGAATGTCATATCATATCATATCGTTATTGTTACTATTATTATTTCCTTTCTTTCTTTATATTTCTACTTTGGGATATTGCGAATAACTAATATATGAAATAATAAATATTCC

Coding sequence (CDS)

ATGGAGAAAATCCAGCACTCAACCATCGCCACCAATGGCATTAACATCCACGTCGCTTCCATTGGCTCTGGTCCGCCAGTGCTATTCCTCCACGGTTTCCCGGAGATTTGGTACACATGGCGCCACCAGCTCCTCTTTCTCGCTTCCAAGGGCTTCCGAGCCATAGCACCTGACCTCCGTGGCTTCGGCGACTCCGATGCGCCACCGTCTCCCTCCTCCTATACGCCTCTCTACATCATTGGCGACCTTATTGGCCTCCTCGATCATCTCGAAATCGATAAAGTTTTCTTGGTAGGCCACGATTGGGGCGCTATGATGGCTTGGTACTTTTGCCTTTTTAGGCCATATCGAGTCAAGGCTTTGGTCAACTTGAGTATCAATTACTTTCCTCGGAATCCGATAATGTCGCTTCTGACCACTGCCCGCATGTTCCTCGGCGATGATTTCTACATTGATGAATCGGAAATTGTGAATTTCGGAACAGGAACCTGGAGAGGCGGAAGCCGATTTTGGGTCTGCAGATACGGCTACGATGATAAACTCCTGACAATGAGAGATCCAAGCCCTCCGATTATACCTAACGGAATCAAAGTACTAGCAACTCCAGAAACCTTACCTCCATGGTTAACAGAGGAAGATATCGATTATTACGCCTCCAAATTCGCCAAAACTGGCTTCACTGGCGGATTTAACTACTATCGCGCCATTGACCTTTGGGAGCTCACAGGGCCATGGACTGGAGCACAGATCAAAGTTCCTACGAAATTCATAGTGGGGGATTTGGATTTGGCGTACCATTTTCCAGGTGTGAACGAGTATATTCATGATGGCGAATTCAAGAACGACGTACCGCTTCTGGAGGAAGTGGTAGTGATAAAAGGAGCAGCTCACTACATTAACTAA

Protein sequence

MEKIQHSTIATNGINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLRGFGDSDAPPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKALVNLSINYFPRNPIMSLLTTARMFLGDDFYIDESEIVNFGTGTWRGGSRFWVCRYGYDDKLLTMRDPSPPIIPNGIKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAIDLWELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVVIKGAAHYIN
Homology
BLAST of Lsi03G010830 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.3e-40
Identity = 105/308 (34.09%), Postives = 151/308 (49.03%), Query Frame = 0

Query: 9   IATNGINIHVASIG--SGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLRGFGDSD 68
           + TNG+ + V   G    P V+  HGFPE+ Y+WRHQ+  LA  G+  +APD RG+G S 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 69  APPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKALVNLSI 128
            P +  +Y    +  DL+GLLD +  ++   VGHDWGA++ W   L    RV A+  LS+
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 129 NYFPRNPIMSLLTTARMFLGDDFY--------IDESEIVNFGTGTWR---GGSRFWVCRY 188
              PR  +         F  + FY        I ++E+      T R   GG R      
Sbjct: 129 PALPRAQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLR------ 188

Query: 189 GYDDKLLTMRDPSPPIIPNG-IKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNYYR 248
              D+   MR  +P   P+G I  L  P  LP W+++E++D+Y  +F +TGFTGG N+YR
Sbjct: 189 PPGDQSAAMRMLAPG--PDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYR 248

Query: 249 AIDL-WELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNE--YIHDGEFKNDVPLLEEVVV 300
             D  WE T    G  I VP+ FI G  D    F   +    +  G ++         V+
Sbjct: 249 NFDRNWETTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISGPYRE--------VL 300

BLAST of Lsi03G010830 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 161.8 bits (408), Expect = 1.2e-38
Identity = 85/273 (31.14%), Postives = 144/273 (52.75%), Query Frame = 0

Query: 14  INIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLRGFGDSDAPPSPSS 73
           + +H   +GSGP V   HGFPE WY+WR+Q+  LA  G+R +A D++G+G+S APP    
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKALVNLSINYFPRNP 133
           Y    +  +++  LD L + +   +GHDWG M+ WY  LF P RV+A+ +L+  + P NP
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 IMSLLTTARMFLGDDFY-------IDESEIVNFGTGTWRGGSRFWVCRYGYDDKLLTMRD 193
            MS L + +     D+        + E+E+    + T++   R        D+ +L+M  
Sbjct: 368 NMSPLESIKANPVFDYQLYFQEPGVAEAELEQNLSRTFKSLFR------ASDESVLSMH- 427

Query: 194 PSPPIIPNGIKVLATPE--TLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAIDL-WELTG 253
               +   G   + +PE  +L   +TEE+I +Y  +F K+GF G  N+YR ++  W+   
Sbjct: 428 ---KVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWAC 487

Query: 254 PWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHD 277
              G +I +P   +  + D     P +++++ D
Sbjct: 488 KSLGRKILIPALMVTAEKDFVL-VPQMSQHMED 509

BLAST of Lsi03G010830 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.1e-38
Identity = 84/264 (31.82%), Postives = 139/264 (52.65%), Query Frame = 0

Query: 13  GINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLRGFGDSDAPPSPS 72
           G+ +H   +GSGP V   HGFPE W++WR+Q+  LA  GFR +A D++G+G+S APP   
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKALVNLSINYFPRN 132
            Y+   +  D++  L+ L + +   +GHDWG ++ W   LF P RV+A+ +L+  + P N
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PIMSLLTTARMFLGDDFY-------IDESEIVNFGTGTWRGGSRFWVCRYGYDDKLLTMR 192
           P +S +   +     D+        + E+E+      T++   R       +D+  LT  
Sbjct: 367 PNVSPMEIIKANPVFDYQLYFQEPGVAEAELEQNLDRTFKNFFR------AHDETFLT-- 426

Query: 193 DPSPPIIPNGIKVLATPE--TLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAIDL-WELT 252
             +  +   G   + TPE  +L   +TEEDI +Y  +F K+GF G  N+YR ++  W+  
Sbjct: 427 --TNRVRELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWG 486

Query: 253 GPWTGAQIKVPTKFIVGDLDLAYH 267
              +G +I +P   +  + DL  H
Sbjct: 487 CKGSGRKILIPALMVTAENDLVLH 500

BLAST of Lsi03G010830 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.2e-35
Identity = 81/256 (31.64%), Postives = 127/256 (49.61%), Query Frame = 0

Query: 13  GINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLRGFGDSDAPPSPS 72
           GI +H   +GSGP +   HGFPE W++WR+Q+  LA  GFR +A D++G+GDS +PP   
Sbjct: 245 GIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKALVNLSINYFPRN 132
            Y    +  +++  L+ L I +   +GHDW  ++ W   LF P RV+A+ +L+    P N
Sbjct: 305 EYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPN 364

Query: 133 PIMSLLTTARMF--LGDDFYIDESEIVNFGTGTWRGGSRFWVCRYGYDDKLLTMRDPSPP 192
           P +S +   R         Y  E  +           +     R   D  LLT+   +  
Sbjct: 365 PEVSPMEVIRSIPVFNYQLYFQEPGVAEAELEKNMSRTFKSFFRTSDDMGLLTVNKAT-- 424

Query: 193 IIPNGIKVLATPE--TLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAIDL-WELTGPWTG 252
               G  ++ TPE   +    TEE+I+YY  +F K+GF G  N+YR  +  W+ +    G
Sbjct: 425 --EMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALG 484

Query: 253 AQIKVPTKFIVGDLDL 264
            +I VP   +  + D+
Sbjct: 485 RKILVPALMVTAEKDI 496

BLAST of Lsi03G010830 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 150.2 bits (378), Expect = 3.7e-35
Identity = 82/256 (32.03%), Postives = 129/256 (50.39%), Query Frame = 0

Query: 13  GINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLRGFGDSDAPPSPS 72
           GI +H   +GSGP +   HGFPE W++WR+Q+  LA  GFR +A D++G+GDS +PP   
Sbjct: 245 GIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKALVNLSINYFPRN 132
            Y    +  +++  LD L I +   +GHDW  +M W   LF P RV+A+ +L+  + P +
Sbjct: 305 EYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPD 364

Query: 133 PIMSLLTTARMF--LGDDFYIDESEIVNFGTGTWRGGSRFWVCRYGYDDKLLTMRDPSPP 192
           P +S +   R         Y  E  +        +  SR +   +   D+   +      
Sbjct: 365 PDVSPMKVIRSIPVFNYQLYFQEPGVAE--AELEKNMSRTFKSFFRASDETGFIAVHKAT 424

Query: 193 IIPNGIKVLATPE--TLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAIDL-WELTGPWTG 252
            I  G  ++ TPE   L    TEE+I++Y  +F KTGF G  N+YR  +  W+ +    G
Sbjct: 425 EI--GGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLG 484

Query: 253 AQIKVPTKFIVGDLDL 264
            +I VP   +  + D+
Sbjct: 485 RKILVPALMVTAEKDI 496

BLAST of Lsi03G010830 vs. ExPASy TrEMBL
Match: A0A6J1J107 (uncharacterized protein LOC111482470 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482470 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.4e-132
Identity = 224/301 (74.42%), Postives = 253/301 (84.05%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLR 60
           ME+I+HSTIATNGIN+HVA IGSGPPVLFLHGFPE+WY+WRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEQIEHSTIATNGINMHVAFIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKA 120
           GFGDSD PPSPSSYTP +IIGDLIGLLDHL ID+VFLVGHDWGAMMAWYFCLFRP RVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSINYFPRNPIMSLLTTARMFLGDDFYIDESEIVNFGTGTWRGGSRFWVCRYGYDDK 180
           LVNLS++Y PRNP +S L+  R FLGDDFYI + +        +       + +     K
Sbjct: 121 LVNLSVHYTPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMK-----K 180

Query: 181 LLTMRDPSPPIIPNGIKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAID-LW 240
            LT+RDPSPPIIPNG K L TPE+LPPWLTEEDIDY+ASKF KTGFTGGFNYYRAI+  W
Sbjct: 181 FLTIRDPSPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTW 240

Query: 241 ELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVVIKGAAHYI 300
           ELTGPW+GA+IKVPTKF+VGD+D+ YH PG  +YIH G FK DVPLLEEVVV++GAAH+I
Sbjct: 241 ELTGPWSGAKIKVPTKFVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFI 296

BLAST of Lsi03G010830 vs. ExPASy TrEMBL
Match: A0A6J1J7K4 (uncharacterized protein LOC111482470 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482470 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.4e-132
Identity = 224/301 (74.42%), Postives = 253/301 (84.05%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLR 60
           ME+I+HSTIATNGIN+HVA IGSGPPVLFLHGFPE+WY+WRHQLLFLASKGFRAIAPDLR
Sbjct: 345 MEQIEHSTIATNGINMHVAFIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 404

Query: 61  GFGDSDAPPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKA 120
           GFGDSD PPSPSSYTP +IIGDLIGLLDHL ID+VFLVGHDWGAMMAWYFCLFRP RVKA
Sbjct: 405 GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 464

Query: 121 LVNLSINYFPRNPIMSLLTTARMFLGDDFYIDESEIVNFGTGTWRGGSRFWVCRYGYDDK 180
           LVNLS++Y PRNP +S L+  R FLGDDFYI + +        +       + +     K
Sbjct: 465 LVNLSVHYTPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMK-----K 524

Query: 181 LLTMRDPSPPIIPNGIKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAID-LW 240
            LT+RDPSPPIIPNG K L TPE+LPPWLTEEDIDY+ASKF KTGFTGGFNYYRAI+  W
Sbjct: 525 FLTIRDPSPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTW 584

Query: 241 ELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVVIKGAAHYI 300
           ELTGPW+GA+IKVPTKF+VGD+D+ YH PG  +YIH G FK DVPLLEEVVV++GAAH+I
Sbjct: 585 ELTGPWSGAKIKVPTKFVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFI 640

BLAST of Lsi03G010830 vs. ExPASy TrEMBL
Match: A0A6J1F1K3 (uncharacterized protein LOC111441542 OS=Cucurbita moschata OX=3662 GN=LOC111441542 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.4e-132
Identity = 224/301 (74.42%), Postives = 253/301 (84.05%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLR 60
           MEKI+HSTIATNGIN+HVASIGSGPPVLFLHGFPE+WY+WRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEKIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKA 120
           GFGDSD PPSPSSYTP +IIGDLIGLLDHL I++VFLVGHDWGAMMAWYFCLFRP RVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIEQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSINYFPRNPIMSLLTTARMFLGDDFYIDESEIVNFGTGTWRGGSRFWVCRYGYDDK 180
           LVNLS++Y PRNP +S L+  R FLGDDFYI + +        +       + +     K
Sbjct: 121 LVNLSVHYNPRNPAISPLSRTRQFLGDDFYICKFQTPGVAEADFGSVDTATMMK-----K 180

Query: 181 LLTMRDPSPPIIPNGIKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAID-LW 240
            LT+RDPSPPIIPNG K L TPETLP WLTEEDIDY+ASKF KTGFTGGFNYYRAI+  W
Sbjct: 181 FLTIRDPSPPIIPNGFKTLKTPETLPSWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTW 240

Query: 241 ELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVVIKGAAHYI 300
           ELTGPW+GA+IKVPTK++VGD+D+ YH PG  +YIH G FK DVPLLEEVVV++GAAH+I
Sbjct: 241 ELTGPWSGAKIKVPTKYVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFI 296

BLAST of Lsi03G010830 vs. ExPASy TrEMBL
Match: A0A6J1F2B6 (uncharacterized protein LOC111441541 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441541 PE=4 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 4.9e-131
Identity = 223/301 (74.09%), Postives = 250/301 (83.06%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLR 60
           ME+I+HSTIATNGIN+HVASIGSGPPVLFLHGFPE+WY+WRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEEIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKA 120
           GFGDSD PPSPSSYTP +IIGDLIGLLDHL ID+VFLVGHDWGAMMAWYFCLFRP RVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSINYFPRNPIMSLLTTARMFLGDDFYIDESEIVNFGTGTWRGGSRFWVCRYGYDDK 180
           LVNLS++Y PRNP +S L+  R  LGDDFYI + +        +       + +     K
Sbjct: 121 LVNLSVHYTPRNPAISTLSLIRKLLGDDFYICKFQKPGVAEADFGSVDTATMMK-----K 180

Query: 181 LLTMRDPSPPIIPNGIKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAIDL-W 240
            LT+RDP PPIIPNG K L TPE+LPPWLTEEDIDY+ASKF KTGFTGG NYYRA DL W
Sbjct: 181 FLTIRDPRPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFEKTGFTGGLNYYRAFDLTW 240

Query: 241 ELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVVIKGAAHYI 300
           E+TGPWTGAQIKVP KF+VGDLD+ Y+ PG  +YIH G FK DVPLLEEVVV++GAAH+I
Sbjct: 241 EMTGPWTGAQIKVPAKFVVGDLDMVYNSPGAKQYIHGGRFKKDVPLLEEVVVMEGAAHFI 296

BLAST of Lsi03G010830 vs. ExPASy TrEMBL
Match: A0A5A7TQJ9 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001150 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 5.0e-128
Identity = 224/302 (74.17%), Postives = 250/302 (82.78%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLR 60
           M+KIQHSTI+TNGINIH ASIGSGP VLFLHGFPE+WY+WRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKA 120
           GFGDSDAPPSPSSYTP +I+GDLIGLLDHL ID+VFLVGHDWGAMMAWYFCLFRP RVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSINYFPRNPIMSLLTTARMFLGDDFYIDESEIVNFGTGTWRGGSRFWVCRYGYDDK 180
           LVNLS++Y PRNP  S L   R +LGDDFYI + +        +       + +     K
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQEPGVAEADFGSVDTATMMK-----K 180

Query: 181 LLTMRDPSPPIIPNGIK-VLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAIDL- 240
            LTMRDP P IIPNG K +L TPE LP WLTEEDI+Y+ASKF+KTGFTGGFNYYRA+D+ 
Sbjct: 181 FLTMRDPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFSKTGFTGGFNYYRALDIT 240

Query: 241 WELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVVIKGAAHY 300
           WELTGPW+ AQIKVPTKFIVGDLDL Y+FPG  EYIH G FK DVPLLE+VVVI+GAAH+
Sbjct: 241 WELTGPWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKKDVPLLEDVVVIEGAAHF 297

BLAST of Lsi03G010830 vs. NCBI nr
Match: XP_022934351.1 (uncharacterized protein LOC111441542 [Cucurbita moschata])

HSP 1 Score: 481.1 bits (1237), Expect = 7.0e-132
Identity = 224/301 (74.42%), Postives = 253/301 (84.05%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLR 60
           MEKI+HSTIATNGIN+HVASIGSGPPVLFLHGFPE+WY+WRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEKIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKA 120
           GFGDSD PPSPSSYTP +IIGDLIGLLDHL I++VFLVGHDWGAMMAWYFCLFRP RVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIEQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSINYFPRNPIMSLLTTARMFLGDDFYIDESEIVNFGTGTWRGGSRFWVCRYGYDDK 180
           LVNLS++Y PRNP +S L+  R FLGDDFYI + +        +       + +     K
Sbjct: 121 LVNLSVHYNPRNPAISPLSRTRQFLGDDFYICKFQTPGVAEADFGSVDTATMMK-----K 180

Query: 181 LLTMRDPSPPIIPNGIKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAID-LW 240
            LT+RDPSPPIIPNG K L TPETLP WLTEEDIDY+ASKF KTGFTGGFNYYRAI+  W
Sbjct: 181 FLTIRDPSPPIIPNGFKTLKTPETLPSWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTW 240

Query: 241 ELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVVIKGAAHYI 300
           ELTGPW+GA+IKVPTK++VGD+D+ YH PG  +YIH G FK DVPLLEEVVV++GAAH+I
Sbjct: 241 ELTGPWSGAKIKVPTKYVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFI 296

BLAST of Lsi03G010830 vs. NCBI nr
Match: XP_022984035.1 (uncharacterized protein LOC111482470 isoform X1 [Cucurbita maxima])

HSP 1 Score: 481.1 bits (1237), Expect = 7.0e-132
Identity = 224/301 (74.42%), Postives = 253/301 (84.05%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLR 60
           ME+I+HSTIATNGIN+HVA IGSGPPVLFLHGFPE+WY+WRHQLLFLASKGFRAIAPDLR
Sbjct: 345 MEQIEHSTIATNGINMHVAFIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 404

Query: 61  GFGDSDAPPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKA 120
           GFGDSD PPSPSSYTP +IIGDLIGLLDHL ID+VFLVGHDWGAMMAWYFCLFRP RVKA
Sbjct: 405 GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 464

Query: 121 LVNLSINYFPRNPIMSLLTTARMFLGDDFYIDESEIVNFGTGTWRGGSRFWVCRYGYDDK 180
           LVNLS++Y PRNP +S L+  R FLGDDFYI + +        +       + +     K
Sbjct: 465 LVNLSVHYTPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMK-----K 524

Query: 181 LLTMRDPSPPIIPNGIKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAID-LW 240
            LT+RDPSPPIIPNG K L TPE+LPPWLTEEDIDY+ASKF KTGFTGGFNYYRAI+  W
Sbjct: 525 FLTIRDPSPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTW 584

Query: 241 ELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVVIKGAAHYI 300
           ELTGPW+GA+IKVPTKF+VGD+D+ YH PG  +YIH G FK DVPLLEEVVV++GAAH+I
Sbjct: 585 ELTGPWSGAKIKVPTKFVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFI 640

BLAST of Lsi03G010830 vs. NCBI nr
Match: XP_022984036.1 (uncharacterized protein LOC111482470 isoform X2 [Cucurbita maxima] >XP_022984037.1 uncharacterized protein LOC111482470 isoform X2 [Cucurbita maxima])

HSP 1 Score: 481.1 bits (1237), Expect = 7.0e-132
Identity = 224/301 (74.42%), Postives = 253/301 (84.05%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLR 60
           ME+I+HSTIATNGIN+HVA IGSGPPVLFLHGFPE+WY+WRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEQIEHSTIATNGINMHVAFIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKA 120
           GFGDSD PPSPSSYTP +IIGDLIGLLDHL ID+VFLVGHDWGAMMAWYFCLFRP RVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSINYFPRNPIMSLLTTARMFLGDDFYIDESEIVNFGTGTWRGGSRFWVCRYGYDDK 180
           LVNLS++Y PRNP +S L+  R FLGDDFYI + +        +       + +     K
Sbjct: 121 LVNLSVHYTPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMK-----K 180

Query: 181 LLTMRDPSPPIIPNGIKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAID-LW 240
            LT+RDPSPPIIPNG K L TPE+LPPWLTEEDIDY+ASKF KTGFTGGFNYYRAI+  W
Sbjct: 181 FLTIRDPSPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTW 240

Query: 241 ELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVVIKGAAHYI 300
           ELTGPW+GA+IKVPTKF+VGD+D+ YH PG  +YIH G FK DVPLLEEVVV++GAAH+I
Sbjct: 241 ELTGPWSGAKIKVPTKFVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFI 296

BLAST of Lsi03G010830 vs. NCBI nr
Match: XP_038905064.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 479.9 bits (1234), Expect = 1.6e-131
Identity = 232/309 (75.08%), Postives = 250/309 (80.91%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLR 60
           M KIQHSTI TNGINIHVASIGSGP VLFLHGFPE+WY+WRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKA 120
           GFGDSD  PSPSSYTP +I+GDLIGLLDHL ID+VFLVGHDWGAMMAWYFCLFRP RVKA
Sbjct: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSINYFPRNPIMSLLTTARMFLGDDFY--------IDESEIVNFGTGTWRGGSRFWV 180
           LVNLS++Y PRNP +S L   R+ LGD+FY        + E++  +  T T         
Sbjct: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMM------- 180

Query: 181 CRYGYDDKLLTMRDPSPPIIPNGIKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNY 240
                  K LTMRDPSPPIIPNG K LATPETLPPWLTEEDIDY+ASKF KTGFTGGFNY
Sbjct: 181 ------KKFLTMRDPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNY 240

Query: 241 YRAIDL-WELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVV 300
           YRA DL WELTGPWTGAQIKVPTKFIVGDLD+ YH PG  EYIH G FK  VPLLEEVVV
Sbjct: 241 YRATDLSWELTGPWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVV 296

BLAST of Lsi03G010830 vs. NCBI nr
Match: KAG6581276.1 (hypothetical protein SDJN03_21278, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 479.6 bits (1233), Expect = 2.0e-131
Identity = 225/301 (74.75%), Postives = 250/301 (83.06%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLR 60
           ME+I+HSTIATNGIN+HVASIGSGPPVLFLHGFPE+WY+WRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEEIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKA 120
           GFGDSD PPSPSSYTP +IIGDLIGLLDHL ID+VFLVGHDWGAMMAWYFCLFRP RVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSINYFPRNPIMSLLTTARMFLGDDFYIDESEIVNFGTGTWRGGSRFWVCRYGYDDK 180
           LVNLS++Y PRNP +S L+  R  LGDDFYI + +        +       + +     K
Sbjct: 121 LVNLSVHYTPRNPAISTLSVIRKLLGDDFYICKFQKPGVAEADFGSVDTATMMK-----K 180

Query: 181 LLTMRDPSPPIIPNGIKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAIDL-W 240
            LT+RDP PPIIPNG K L TPE+LPPWLTEEDIDY+ASKF KTGFTGG NYYRA DL W
Sbjct: 181 FLTIRDPRPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFEKTGFTGGLNYYRAFDLTW 240

Query: 241 ELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVVIKGAAHYI 300
           ELTGPWTGAQIKVP KF+VGDLD+ Y+ PG  +YIH G FK DVPLLEEVVVI+GAAH+I
Sbjct: 241 ELTGPWTGAQIKVPAKFVVGDLDMVYNSPGAKQYIHGGRFKKDVPLLEEVVVIEGAAHFI 296

BLAST of Lsi03G010830 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 369.8 bits (948), Expect = 2.1e-102
Identity = 171/304 (56.25%), Postives = 222/304 (73.03%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVASIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLR 60
           MEKI+H+TI+TNGIN+HVASIGSGP +LF+HGFP++WY+WRHQL+  A+ G+RAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GFGDSDAPPSPSSYTPLYIIGDLIGLLDHLEIDKVFLVGHDWGAMMAWYFCLFRPYRVKA 120
           G+GDSDAPPS  SYT L+I+GDL+GLLD L +D+VFLVGHDWGA++AW+ C+ RP RV A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 LVNLSINYFPRNPIMSLLTTARMFLGDDFYIDE-SEIVNFGTGTWRGGSRFWVCRYGYDD 180
           LVN S+ + PRNP +  +   R   GDD+YI    E         +  ++  + R+    
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRF---- 180

Query: 181 KLLTMRDPSPPIIPN--GIKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNYYRAID 240
              T R+P PP IP   G + L  P +LP WLTE+D+ +Y  KF++ GFTGG NYYRA++
Sbjct: 181 --FTSRNPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALN 240

Query: 241 L-WELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVVIKGAA 300
           L WELT PWTG QIKVP KFIVGDLD+ Y+ PG  EYIH+G  K  VP L+EVVV++G  
Sbjct: 241 LSWELTAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVG 298

BLAST of Lsi03G010830 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 315.1 bits (806), Expect = 6.2e-86
Identity = 165/318 (51.89%), Postives = 209/318 (65.72%), Query Frame = 0

Query: 4   IQHSTIATNGINIHVASIG--SGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPDLRG 63
           ++H  +  NGI+IHVA  G   GP VL LHGFPE+WY+WRHQ+  LA++G+RA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  FGDSDAPPSPSSYTPLYIIGDLIGLLDHL---EIDKVFLVGHDWGAMMAWYFCLFRPYRV 123
           +GDSDAP   SSYT   I+GDLI ++  L   E +KVF+VGHDWGA++AWY CLFRP RV
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KALVNLSI--NYFPRNPIMSLLTTARMFLGDDFYI--------DESEIVNFGTGTWRGGS 183
           KALVNLS+  ++ P +P +  +   R F GDD+YI         E+EI   GT       
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTER----- 180

Query: 184 RFWVCRYGYDDKLLTMRDPSPPIIPN-----GIKVLATPETLPPWLTEEDIDYYASKFAK 243
                      +LLT R P P IIP      G K    P  LP WLTEED+ Y+ SKF +
Sbjct: 181 --------VMKRLLTYRTPGPVIIPKDKSFWGSKGETIP--LPSWLTEEDVAYFVSKFEE 240

Query: 244 TGFTGGFNYYRAIDL-WELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKND 301
            GF+G  NYYR  +   EL GPW G++I+VPTKF++G+LDL Y+ PGV EYIH  +FK D
Sbjct: 241 KGFSGPVNYYRNFNRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKED 300

BLAST of Lsi03G010830 vs. TAIR 10
Match: AT4G15955.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 309.3 bits (791), Expect = 3.4e-84
Identity = 153/309 (49.51%), Postives = 201/309 (65.05%), Query Frame = 0

Query: 6   HSTIATNGINIHVAS-----IGSG----PPVLFLHGFPEIWYTWRHQLLFLASKGFRAIA 65
           HS +  NGI +HVA       G+G    P +LFLHGFPE+WYTWRHQ++ L+S G+R IA
Sbjct: 7   HSFVKVNGITMHVAEKSPSVAGNGAIRPPVILFLHGFPELWYTWRHQMVALSSLGYRTIA 66

Query: 66  PDLRGFGDSDAPPSPSSYTPLYIIGDLIGLLDHL--EIDKVFLVGHDWGAMMAWYFCLFR 125
           PDLRG+GD+DAP S  +YT L+++GDLIGL+D +  + +KVF+VGHDWGA++AW+ CLFR
Sbjct: 67  PDLRGYGDTDAPESVDAYTSLHVVGDLIGLIDAVVGDREKVFVVGHDWGAIIAWHLCLFR 126

Query: 126 PYRVKALVNLSINYFPRNPIMSLLTTARMFLGDDFYIDESEIVNFGTGTWRGGSRFWVCR 185
           P RVKALVN+S+ + P NP     +T + F GDD+YI   +++           +  VC 
Sbjct: 127 PDRVKALVNMSVVFDPWNPKRKPTSTFKAFYGDDYYICRFQLLEILI-------KIHVCI 186

Query: 186 YG--YDDKLLTMRDPSPPIIPNGIKVLATPETLPPWLTEEDIDYYASKFAKTGFTGGFNY 245
            G  YDD +                      +LP WLT+ D+ YY SK+ K GFTG  NY
Sbjct: 187 VGKRYDDSV----------------------SLPSWLTDSDVKYYVSKYEKNGFTGPVNY 246

Query: 246 YRAID-LWELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEVVV 301
           YR +D  WEL G  + A++KVP KFI+GD DL YH PG  +YIHDG FK+ VPLL+EVVV
Sbjct: 247 YRNMDRTWELMGSLSNAKVKVPVKFIIGDQDLTYHIPGSKKYIHDGRFKSHVPLLDEVVV 286

BLAST of Lsi03G010830 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 308.9 bits (790), Expect = 4.4e-84
Identity = 153/311 (49.20%), Postives = 198/311 (63.67%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVASIG--SGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAIAPD 60
           ME I H  ++ NGI +H+A  G   GP VL LHGFP++WYTWRHQ+  L+S G+RA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGFGDSDAPPSPSSYTPLYIIGDLIGLLDHL--EIDKVFLVGHDWGAMMAWYFCLFRPY 120
           LRG+GDSD+P S S YT L ++GDL+ LLD +    +KVFLVGHDWGA++ W+ CLFRP 
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RVKALVNLSINYFPRNPIMSLLTTARMFLGDDFYIDESEIVNFGTGTWRGGSRFWVCRYG 180
           ++   V LS+ Y  RNP +  +   +   GDD+YI   +      G           R  
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLR-- 180

Query: 181 YDDKLLTMRDPSPPIIPN----GIKVLATPET--LPPWLTEEDIDYYASKFAKTGFTGGF 240
               L T R   PPI+P     G K     E   LP W +++D+D+Y SKF K GFTGG 
Sbjct: 181 ---NLFTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGL 240

Query: 241 NYYRAIDL-WELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFKNDVPLLEEV 300
           NYYRA+DL WELT PWTGA+I+VP KF+ GD D+ Y  PG+ EYIH G F  DVP L+E+
Sbjct: 241 NYYRAMDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEI 300

BLAST of Lsi03G010830 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 3.7e-83
Identity = 153/320 (47.81%), Postives = 202/320 (63.12%), Query Frame = 0

Query: 1   MEKIQHSTIATNGINIHVA-----SIGSGPPVLFLHGFPEIWYTWRHQLLFLASKGFRAI 60
           ++ ++H T+  NGIN+HVA       G  P +LFLHGFPE+WYTWRHQ++ L+S G+R I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGFGDSDAPPSPSSYTPLYIIGDLIGLLDHLE-IDK-VFLVGHDWGAMMAWYFCLF 120
           APDLRG+GD++AP     YT L + GD++ L+D +   DK V +VGHDWGAM+AW  C +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPYRVKALVNLSINYFPRNPIMSLLTTARMFLGDDFYI--------DESEIVNFGTGTWR 180
           RP +VKALVN+S+ + PRNP+   + T R   GDD+Y+         E+E    GT    
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGT---- 230

Query: 181 GGSRFWVCRYGYDDKLLTMRDPSPPIIPNGIKVLATPE----TLPPWLTEEDIDYYASKF 240
                         + LT + P P  +P   K     E     LP WLT+ED+DYY +K+
Sbjct: 231 ---------ENVLKEFLTYKTPGPLNLPKD-KYFKRSENAASALPLWLTQEDLDYYVTKY 290

Query: 241 AKTGFTGGFNYYRAIDL-WELTGPWTGAQIKVPTKFIVGDLDLAYHFPGVNEYIHDGEFK 300
              GFTG  NYYR ID  WELT PWTGA+I+VP KFI+GD DL Y+FPG  EYI+ G FK
Sbjct: 291 ENKGFTGPINYYRNIDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFK 350

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
I6YGS01.3e-4034.09Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
P349131.2e-3831.14Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
Q6Q2C26.1e-3831.82Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P802992.2e-3531.64Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
P349143.7e-3532.03Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1J1073.4e-13274.42uncharacterized protein LOC111482470 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1J7K43.4e-13274.42uncharacterized protein LOC111482470 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1F1K33.4e-13274.42uncharacterized protein LOC111441542 OS=Cucurbita moschata OX=3662 GN=LOC1114415... [more]
A0A6J1F2B64.9e-13174.09uncharacterized protein LOC111441541 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A5A7TQJ95.0e-12874.17Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
Match NameE-valueIdentityDescription
XP_022934351.17.0e-13274.42uncharacterized protein LOC111441542 [Cucurbita moschata][more]
XP_022984035.17.0e-13274.42uncharacterized protein LOC111482470 isoform X1 [Cucurbita maxima][more]
XP_022984036.17.0e-13274.42uncharacterized protein LOC111482470 isoform X2 [Cucurbita maxima] >XP_022984037... [more]
XP_038905064.11.6e-13175.08epoxide hydrolase A-like [Benincasa hispida][more]
KAG6581276.12.0e-13174.75hypothetical protein SDJN03_21278, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT4G02340.12.1e-10256.25alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.16.2e-8651.89soluble epoxide hydrolase [more]
AT4G15955.33.4e-8449.51alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.14.4e-8449.20alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.13.7e-8347.81alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 97..110
score: 48.7
coord: 51..66
score: 56.82
coord: 252..268
score: 41.71
coord: 31..49
score: 51.2
coord: 111..124
score: 52.6
coord: 292..300
score: 12.06
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 97..110
score: 31.03
coord: 111..124
score: 28.08
coord: 51..66
score: 51.51
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 25..136
e-value: 5.7E-23
score: 81.9
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..300
e-value: 1.2E-84
score: 286.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 2..299
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..300
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G010830.1Lsi03G010830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity