Lsi03G010590 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G010590
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr03: 20337529 .. 20339367 (+)
RNA-Seq ExpressionLsi03G010590
SyntenyLsi03G010590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATATTTCTCATTTTCTTCACCAACTCCAACTCTGCGCCAGACGCCAATCGGCATCAGCCGCTGGAAAACTCCATTCTCAGATCATCAAAGCCGGTTTCGACCAATCGGGCCCTCTCCCAAACACCCTTTTGGATGTATATGGCAAATGCGGTCTCATTCCACAAGCCCTCCAACTGTTCGACGAAATGCCCCACAGAGACCATGTCACTTGGGCCTCGATTCTCACTGCCCATAACCAAGCCATTCTTCCCCGACGGACCCTTTCAATGCTTAACACCATGTTTACGCTTGATGGGTTGCAGCCTGATCACTTTGTCTTCGCCTGCATTGTCAGGGCCTGCTCCAGTTTGGGGTATTTGCGGCTAGGTAAGCAAGTTCATGCTCGGTTTATGTTGTCTTCTTTTTGTGAGGATGAGGTTGTTAAGTCGTCTTTGATTGATATGTATGCTAAATGCGCGCAACCCGATGATGCTCGGGCCGTTTTTGATTCCATATTGTTTAAGAATTCCGTTTCTTGGACTGCTATGATTTCTGGGTATGCTAGAAGTGGTAGGAAATGTGAGGCTATTGAGCTTTTTCAGCGAGTTCCTGTGAGAAACTTGTTCTCTTGGACTGCTTTGATTTCGGGGTTGATTCAAAGTGGGAATGGAATTTACTCGTTATCTTTGTTTAATGAGATGAGGAGAGAAGGAATTGATATTGTAGACCCTTTAGTTCTTTCAAGTGTGGTTGGAGCTTGTGCAAGTGCAAATTTGGCTCTTCTGGAGCTTGGAAAGCAAATTCATGGCTTGGTTATAGCTCTTGGCTTTGAGTCTTGTTTGTTTATAAGCAATGCACTCGTGGATATGTATGCTAAATGTAGTGACATATTAGCAGCTAAGGATATTTTTTACAGAATGCCACGAAAGGATGTGGTTTCTTGGACTTCAATCATAGTTGGGACTGCTCAACATGGTAAGGCAGAGGAAGCATTAACTTTATATGATGAGATGGTTCTTTCCGGAATAAAGCCGAATGAAGTGACTTTCATTGGATTACTTTATGCTTGCAGTCACGTCGGGCTAGTTAGCAGAGGTCGCGAATTATTTAGGTCTATGACAAATGATTATAGGATCAATCCTTCTCTACAGCACTACACATGTTTATTGGATCTCCTGAGTCGCTCTGGACACCTTGATGAGGCAGAGAATCTACTCGACGAAATGCCATTTAAACCCGATGAGCCTACTTGGGCATCGTTATTGAGCGCATGCAAGCGACATAACAATTTGAAAATGGGAGTTCGAATTGCTGATAATGTGTTGGATTTAAAACCTGAAGATCCTTCAACATACATACTGTTATCGAACGTTTATGCTGGAGCTGAAATGTGGGAGAGTGTTTCAAAGGTGAGAAAGTTGATGAGTTCTATGGAAGTAAGAAAGGAACCAGGTTATAGCAGCATTGACTTTGGAAAGGAGAGCCAAGTATTTCATGCTGGGGAGGGTTGTCATCCTATGAAGGATGAGATTTTCAATCTGCTGAAGGATTTAGATGCAGAAATGAGAAGAAGAGGCTATGTTCCTAACACTAGCTTTGTTTTGCATGACATGGAGCAACAAGAAAAGGAAAAGCAGCTCTTTTGGCATAGTGAGAGGTTGGCTGTGGCTTATGGACTTCTCAAGGCTGTTCCTGGAACGATTATTCGAATTGTGAAAAATCTTCGCATTTGTGGAGATTGTCATAATGTTTTGAAATTCACTAGTGATATAAAGCAAAGAGAAATTGTGGTCCGTGATGCCACGAGATATCATCATTTCAAGGAAGGAAAATGTTCTTGCAATGATTTCTGGTGA

mRNA sequence

ATGAATATTTCTCATTTTCTTCACCAACTCCAACTCTGCGCCAGACGCCAATCGGCATCAGCCGCTGGAAAACTCCATTCTCAGATCATCAAAGCCGGTTTCGACCAATCGGGCCCTCTCCCAAACACCCTTTTGGATGTATATGGCAAATGCGGTCTCATTCCACAAGCCCTCCAACTGTTCGACGAAATGCCCCACAGAGACCATGTCACTTGGGCCTCGATTCTCACTGCCCATAACCAAGCCATTCTTCCCCGACGGACCCTTTCAATGCTTAACACCATGTTTACGCTTGATGGGTTGCAGCCTGATCACTTTGTCTTCGCCTGCATTGTCAGGGCCTGCTCCAGTTTGGGGTATTTGCGGCTAGGTAAGCAAGTTCATGCTCGGTTTATGTTGTCTTCTTTTTGTGAGGATGAGGTTGTTAAGTCGTCTTTGATTGATATGTATGCTAAATGCGCGCAACCCGATGATGCTCGGGCCGTTTTTGATTCCATATTGTTTAAGAATTCCGTTTCTTGGACTGCTATGATTTCTGGGTATGCTAGAAGTGGTAGGAAATGTGAGGCTATTGAGCTTTTTCAGCGAGTTCCTGTGAGAAACTTGTTCTCTTGGACTGCTTTGATTTCGGGGTTGATTCAAAGTGGGAATGGAATTTACTCGTTATCTTTGTTTAATGAGATGAGGAGAGAAGGAATTGATATTGTAGACCCTTTAGTTCTTTCAAGTGTGGTTGGAGCTTGTGCAAGTGCAAATTTGGCTCTTCTGGAGCTTGGAAAGCAAATTCATGGCTTGGTTATAGCTCTTGGCTTTGAGTCTTGTTTGTTTATAAGCAATGCACTCGTGGATATGTATGCTAAATGTAGTGACATATTAGCAGCTAAGGATATTTTTTACAGAATGCCACGAAAGGATGTGGTTTCTTGGACTTCAATCATAGTTGGGACTGCTCAACATGGTAAGGCAGAGGAAGCATTAACTTTATATGATGAGATGGTTCTTTCCGGAATAAAGCCGAATGAAGTGACTTTCATTGGATTACTTTATGCTTGCAGTCACGTCGGGCTAGTTAGCAGAGGTCGCGAATTATTTAGGTCTATGACAAATGATTATAGGATCAATCCTTCTCTACAGCACTACACATGTTTATTGGATCTCCTGAGTCGCTCTGGACACCTTGATGAGGCAGAGAATCTACTCGACGAAATGCCATTTAAACCCGATGAGCCTACTTGGGCATCGTTATTGAGCGCATGCAAGCGACATAACAATTTGAAAATGGGAGTTCGAATTGCTGATAATGTGTTGGATTTAAAACCTGAAGATCCTTCAACATACATACTGTTATCGAACGTTTATGCTGGAGCTGAAATGTGGGAGAGTGTTTCAAAGGTGAGAAAGTTGATGAGTTCTATGGAAGTAAGAAAGGAACCAGGTTATAGCAGCATTGACTTTGGAAAGGAGAGCCAAGTATTTCATGCTGGGGAGGGTTGTCATCCTATGAAGGATGAGATTTTCAATCTGCTGAAGGATTTAGATGCAGAAATGAGAAGAAGAGGCTATGTTCCTAACACTAGCTTTGTTTTGCATGACATGGAGCAACAAGAAAAGGAAAAGCAGCTCTTTTGGCATAGTGAGAGGTTGGCTGTGGCTTATGGACTTCTCAAGGCTGTTCCTGGAACGATTATTCGAATTGTGAAAAATCTTCGCATTTGTGGAGATTGTCATAATGTTTTGAAATTCACTAGTGATATAAAGCAAAGAGAAATTGTGGTCCGTGATGCCACGAGATATCATCATTTCAAGGAAGGAAAATGTTCTTGCAATGATTTCTGGTGA

Coding sequence (CDS)

ATGAATATTTCTCATTTTCTTCACCAACTCCAACTCTGCGCCAGACGCCAATCGGCATCAGCCGCTGGAAAACTCCATTCTCAGATCATCAAAGCCGGTTTCGACCAATCGGGCCCTCTCCCAAACACCCTTTTGGATGTATATGGCAAATGCGGTCTCATTCCACAAGCCCTCCAACTGTTCGACGAAATGCCCCACAGAGACCATGTCACTTGGGCCTCGATTCTCACTGCCCATAACCAAGCCATTCTTCCCCGACGGACCCTTTCAATGCTTAACACCATGTTTACGCTTGATGGGTTGCAGCCTGATCACTTTGTCTTCGCCTGCATTGTCAGGGCCTGCTCCAGTTTGGGGTATTTGCGGCTAGGTAAGCAAGTTCATGCTCGGTTTATGTTGTCTTCTTTTTGTGAGGATGAGGTTGTTAAGTCGTCTTTGATTGATATGTATGCTAAATGCGCGCAACCCGATGATGCTCGGGCCGTTTTTGATTCCATATTGTTTAAGAATTCCGTTTCTTGGACTGCTATGATTTCTGGGTATGCTAGAAGTGGTAGGAAATGTGAGGCTATTGAGCTTTTTCAGCGAGTTCCTGTGAGAAACTTGTTCTCTTGGACTGCTTTGATTTCGGGGTTGATTCAAAGTGGGAATGGAATTTACTCGTTATCTTTGTTTAATGAGATGAGGAGAGAAGGAATTGATATTGTAGACCCTTTAGTTCTTTCAAGTGTGGTTGGAGCTTGTGCAAGTGCAAATTTGGCTCTTCTGGAGCTTGGAAAGCAAATTCATGGCTTGGTTATAGCTCTTGGCTTTGAGTCTTGTTTGTTTATAAGCAATGCACTCGTGGATATGTATGCTAAATGTAGTGACATATTAGCAGCTAAGGATATTTTTTACAGAATGCCACGAAAGGATGTGGTTTCTTGGACTTCAATCATAGTTGGGACTGCTCAACATGGTAAGGCAGAGGAAGCATTAACTTTATATGATGAGATGGTTCTTTCCGGAATAAAGCCGAATGAAGTGACTTTCATTGGATTACTTTATGCTTGCAGTCACGTCGGGCTAGTTAGCAGAGGTCGCGAATTATTTAGGTCTATGACAAATGATTATAGGATCAATCCTTCTCTACAGCACTACACATGTTTATTGGATCTCCTGAGTCGCTCTGGACACCTTGATGAGGCAGAGAATCTACTCGACGAAATGCCATTTAAACCCGATGAGCCTACTTGGGCATCGTTATTGAGCGCATGCAAGCGACATAACAATTTGAAAATGGGAGTTCGAATTGCTGATAATGTGTTGGATTTAAAACCTGAAGATCCTTCAACATACATACTGTTATCGAACGTTTATGCTGGAGCTGAAATGTGGGAGAGTGTTTCAAAGGTGAGAAAGTTGATGAGTTCTATGGAAGTAAGAAAGGAACCAGGTTATAGCAGCATTGACTTTGGAAAGGAGAGCCAAGTATTTCATGCTGGGGAGGGTTGTCATCCTATGAAGGATGAGATTTTCAATCTGCTGAAGGATTTAGATGCAGAAATGAGAAGAAGAGGCTATGTTCCTAACACTAGCTTTGTTTTGCATGACATGGAGCAACAAGAAAAGGAAAAGCAGCTCTTTTGGCATAGTGAGAGGTTGGCTGTGGCTTATGGACTTCTCAAGGCTGTTCCTGGAACGATTATTCGAATTGTGAAAAATCTTCGCATTTGTGGAGATTGTCATAATGTTTTGAAATTCACTAGTGATATAAAGCAAAGAGAAATTGTGGTCCGTGATGCCACGAGATATCATCATTTCAAGGAAGGAAAATGTTCTTGCAATGATTTCTGGTGA

Protein sequence

MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQLFDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGYLRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISGYARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLVLSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYRMPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRGRELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACKRHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGYSSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEKQLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRYHHFKEGKCSCNDFW
Homology
BLAST of Lsi03G010590 vs. ExPASy Swiss-Prot
Match: O23266 (Pentatricopeptide repeat-containing protein At4g14050, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-H13 PE=1 SV=3)

HSP 1 Score: 863.2 bits (2229), Expect = 1.7e-249
Identity = 411/614 (66.94%), Postives = 506/614 (82.41%), Query Frame = 0

Query: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQL 60
           M I H+LHQLQLCAR ++ + A  LH+ I+K G  Q  PL NTL++VYGKCG    ALQ+
Sbjct: 1   MLIPHYLHQLQLCARNRTLTTAKALHAHIVKLGIVQCCPLANTLVNVYGKCGAASHALQV 60

Query: 61  FDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGY 120
           FDEMPHRDH+ WAS+LTA NQA L  +TLS+ +++ +  GL+PD FVF+ +V+AC++LG 
Sbjct: 61  FDEMPHRDHIAWASVLTALNQANLSGKTLSVFSSVGSSSGLRPDDFVFSALVKACANLGS 120

Query: 121 LRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180
           +  G+QVH  F++S +  DEVVKSSL+DMYAKC   + A+AVFDSI  KN++SWTAM+SG
Sbjct: 121 IDHGRQVHCHFIVSEYANDEVVKSSLVDMYAKCGLLNSAKAVFDSIRVKNTISWTAMVSG 180

Query: 181 YARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLV 240
           YA+SGRK EA+ELF+ +PV+NL+SWTALISG +QSG G+ + S+F EMRRE +DI+DPLV
Sbjct: 181 YAKSGRKEEALELFRILPVKNLYSWTALISGFVQSGKGLEAFSVFTEMRRERVDILDPLV 240

Query: 241 LSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300
           LSS+VGAC  ANLA    G+Q+HGLVIALGF+SC+FISNAL+DMYAKCSD++AAKDIF R
Sbjct: 241 LSSIVGAC--ANLAASIAGRQVHGLVIALGFDSCVFISNALIDMYAKCSDVIAAKDIFSR 300

Query: 301 MPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRG 360
           M  +DVVSWTS+IVG AQHG+AE+AL LYD+MV  G+KPNEVTF+GL+YACSHVG V +G
Sbjct: 301 MRHRDVVSWTSLIVGMAQHGQAEKALALYDDMVSHGVKPNEVTFVGLIYACSHVGFVEKG 360

Query: 361 RELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACK 420
           RELF+SMT DY I PSLQHYTCLLDLL RSG LDEAENL+  MPF PDEPTWA+LLSACK
Sbjct: 361 RELFQSMTKDYGIRPSLQHYTCLLDLLGRSGLLDEAENLIHTMPFPPDEPTWAALLSACK 420

Query: 421 RHNNLKMGVRIADN-VLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPG 480
           R    +MG+RIAD+ V   K +DPSTYILLSN+YA A +W  VS+ R+ +  MEVRK+PG
Sbjct: 421 RQGRGQMGIRIADHLVSSFKLKDPSTYILLSNIYASASLWGKVSEARRKLGEMEVRKDPG 480

Query: 481 YSSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMR-RRGYVPNTSFVLHDMEQQEKE 540
           +SS++  KE++VF+AGE  HP+K++IF LLK L+ EMR R GYVP+TS++LHDM++QEKE
Sbjct: 481 HSSVEVRKETEVFYAGETSHPLKEDIFRLLKKLEEEMRIRNGYVPDTSWILHDMDEQEKE 540

Query: 541 KQLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRY 600
           K LFWHSER AVAYGLLKAVPGT IRIVKNLR+CGDCH VLK  S+I +REI+VRDATRY
Sbjct: 541 KLLFWHSERSAVAYGLLKAVPGTPIRIVKNLRVCGDCHVVLKHISEITEREIIVRDATRY 600

Query: 601 HHFKEGKCSCNDFW 613
           HHFK GKCSCNDFW
Sbjct: 601 HHFKGGKCSCNDFW 612

BLAST of Lsi03G010590 vs. ExPASy Swiss-Prot
Match: Q9FRI5 (Pentatricopeptide repeat-containing protein At1g25360 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H74 PE=2 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 6.4e-135
Identity = 251/626 (40.10%), Postives = 368/626 (58.79%), Query Frame = 0

Query: 24  KLHSQIIKAGFDQSGPLPNTLLDVYGKC----GLIPQALQLFDEMPHRDHVTWASILT-- 83
           + H+  +K+G      + N L+ VY KC     L+  A ++FDE+  +D  +W +++T  
Sbjct: 170 QFHAAALKSGAGYITSVSNALVSVYSKCASSPSLLHSARKVFDEILEKDERSWTTMMTGY 229

Query: 84  -----------------------AHNQAI-------LPRRTLSMLNTMFTLDGLQPDHFV 143
                                  A+N  I         +  L M+  M +  G++ D F 
Sbjct: 230 VKNGYFDLGEELLEGMDDNMKLVAYNAMISGYVNRGFYQEALEMVRRMVS-SGIELDEFT 289

Query: 144 FACIVRACSSLGYLRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSIL 203
           +  ++RAC++ G L+LGKQVHA ++L          +SL+ +Y KC + D+ARA+F+ + 
Sbjct: 290 YPSVIRACATAGLLQLGKQVHA-YVLRREDFSFHFDNSLVSLYYKCGKFDEARAIFEKMP 349

Query: 204 FKNSVSWTAMISGYARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNE 263
            K+ VSW A++SGY  SG   EA  +F+ +  +N+ SW  +ISGL ++G G   L LF+ 
Sbjct: 350 AKDLVSWNALLSGYVSSGHIGEAKLIFKEMKEKNILSWMIMISGLAENGFGEEGLKLFSC 409

Query: 264 MRREGIDIVDPLVLSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAK 323
           M+REG +  D    S  + +CA   L     G+Q H  ++ +GF+S L   NAL+ MYAK
Sbjct: 410 MKREGFEPCD-YAFSGAIKSCAV--LGAYCNGQQYHAQLLKIGFDSSLSAGNALITMYAK 469

Query: 324 CSDILAAKDIFYRMPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGL 383
           C  +  A+ +F  MP  D VSW ++I    QHG   EA+ +Y+EM+  GI+P+ +T + +
Sbjct: 470 CGVVEEARQVFRTMPCLDSVSWNALIAALGQHGHGAEAVDVYEEMLKKGIRPDRITLLTV 529

Query: 384 LYACSHVGLVSRGRELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKP 443
           L ACSH GLV +GR+ F SM   YRI P   HY  L+DLL RSG   +AE++++ +PFKP
Sbjct: 530 LTACSHAGLVDQGRKYFDSMETVYRIPPGADHYARLIDLLCRSGKFSDAESVIESLPFKP 589

Query: 444 DEPTWASLLSACKRHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRK 503
               W +LLS C+ H N+++G+  AD +  L PE   TY+LLSN++A    WE V++VRK
Sbjct: 590 TAEIWEALLSGCRVHGNMELGIIAADKLFGLIPEHDGTYMLLSNMHAATGQWEEVARVRK 649

Query: 504 LMSSMEVRKEPGYSSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMRRRGYVPNTSF 563
           LM    V+KE   S I+   +   F   +  HP  + ++  L+DL  EMRR GYVP+TSF
Sbjct: 650 LMRDRGVKKEVACSWIEMETQVHTFLVDDTSHPEAEAVYIYLQDLGKEMRRLGYVPDTSF 709

Query: 564 VLHDMEQQ-EKEKQLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIK 613
           VLHD+E    KE  L  HSE++AVA+GL+K  PGT IRI KNLR CGDCHN  +F S + 
Sbjct: 710 VLHDVESDGHKEDMLTTHSEKIAVAFGLMKLPPGTTIRIFKNLRTCGDCHNFFRFLSWVV 769

BLAST of Lsi03G010590 vs. ExPASy Swiss-Prot
Match: Q9SY02 (Pentatricopeptide repeat-containing protein At4g02750 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H24 PE=3 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 2.4e-129
Identity = 234/572 (40.91%), Postives = 351/572 (61.36%), Query Frame = 0

Query: 42  NTLLDVYGKCGLIPQALQLFDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGL 101
           N LL  + K   I +A Q FD M  RD V+W +I+T + Q+      +     +F    +
Sbjct: 223 NCLLGGFVKKKKIVEARQFFDSMNVRDVVSWNTIITGYAQS----GKIDEARQLFDESPV 282

Query: 102 QPDHFVFACIVRACSSLGYLRLGKQVHARFMLSSFCE-DEVVKSSLIDMYAKCAQPDDAR 161
           Q D F +  +V      GY++      AR +     E +EV  ++++  Y +  + + A+
Sbjct: 283 Q-DVFTWTAMVS-----GYIQNRMVEEARELFDKMPERNEVSWNAMLAGYVQGERMEMAK 342

Query: 162 AVFDSILFKNSVSWTAMISGYARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIY 221
            +FD +  +N  +W  MI+GYA+ G+  EA  LF ++P R+  SW A+I+G  QSG+   
Sbjct: 343 ELFDVMPCRNVSTWNTMITGYAQCGKISEAKNLFDKMPKRDPVSWAAMIAGYSQSGHSFE 402

Query: 222 SLSLFNEMRREGIDIVDPLVLSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNA 281
           +L LF +M REG   ++    SS +  C  A++  LELGKQ+HG ++  G+E+  F+ NA
Sbjct: 403 ALRLFVQMEREG-GRLNRSSFSSALSTC--ADVVALELGKQLHGRLVKGGYETGCFVGNA 462

Query: 282 LVDMYAKCSDILAAKDIFYRMPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPN 341
           L+ MY KC  I  A D+F  M  KD+VSW ++I G ++HG  E AL  ++ M   G+KP+
Sbjct: 463 LLLMYCKCGSIEEANDLFKEMAGKDIVSWNTMIAGYSRHGFGEVALRFFESMKREGLKPD 522

Query: 342 EVTFIGLLYACSHVGLVSRGRELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLL 401
           + T + +L ACSH GLV +GR+ F +MT DY + P+ QHY C++DLL R+G L++A NL+
Sbjct: 523 DATMVAVLSACSHTGLVDKGRQYFYTMTQDYGVMPNSQHYACMVDLLGRAGLLEDAHNLM 582

Query: 402 DEMPFKPDEPTWASLLSACKRHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWE 461
             MPF+PD   W +LL A + H N ++    AD +  ++PE+   Y+LLSN+YA +  W 
Sbjct: 583 KNMPFEPDAAIWGTLLGASRVHGNTELAETAADKIFAMEPENSGMYVLLSNLYASSGRWG 642

Query: 462 SVSKVRKLMSSMEVRKEPGYSSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMRRRG 521
            V K+R  M    V+K PGYS I+   ++  F  G+  HP KDEIF  L++LD  M++ G
Sbjct: 643 DVGKLRVRMRDKGVKKVPGYSWIEIQNKTHTFSVGDEFHPEKDEIFAFLEELDLRMKKAG 702

Query: 522 YVPNTSFVLHDMEQQEKEKQLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLK 581
           YV  TS VLHD+E++EKE+ + +HSERLAVAYG+++   G  IR++KNLR+C DCHN +K
Sbjct: 703 YVSKTSVVLHDVEEEEKERMVRYHSERLAVAYGIMRVSSGRPIRVIKNLRVCEDCHNAIK 762

Query: 582 FTSDIKQREIVVRDATRYHHFKEGKCSCNDFW 613
           + + I  R I++RD  R+HHFK+G CSC D+W
Sbjct: 763 YMARITGRLIILRDNNRFHHFKDGSCSCGDYW 781

BLAST of Lsi03G010590 vs. ExPASy Swiss-Prot
Match: O23169 (Pentatricopeptide repeat-containing protein At4g37170 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H5 PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 2.0e-128
Identity = 236/640 (36.88%), Postives = 365/640 (57.03%), Query Frame = 0

Query: 4   SHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKC------------ 63
           S + + +Q+C++ ++     K+H  I  +GF     + N LL +Y KC            
Sbjct: 86  STYCNLIQVCSQTRALEEGKKVHEHIRTSGFVPGIVIWNRLLRMYAKCGSLVDARKVFDE 145

Query: 64  -------------------GLIPQALQLFDEMPHRDHVTWASILTAHNQAILPRRTLSML 123
                              GL+ +A +LFDEM  +D  +W +++T + +   P   L + 
Sbjct: 146 MPNRDLCSWNVMVNGYAEVGLLEEARKLFDEMTEKDSYSWTAMVTGYVKKDQPEEALVLY 205

Query: 124 NTMFTLDGLQPDHFVFACIVRACSSLGYLRLGKQVHARFMLSSFCEDEVVKSSLIDMYAK 183
           + M  +   +P+ F  +  V A +++  +R GK++H   + +    DEV+ SSL+DMY K
Sbjct: 206 SLMQRVPNSRPNIFTVSIAVAAAAAVKCIRRGKEIHGHIVRAGLDSDEVLWSSLMDMYGK 265

Query: 184 CAQPDDARAVFDSILFKNSVSWTAMISGYARSGRKCEAIELFQRVPVRNLFSWTALISGL 243
           C   D+AR +FD I+ K+ VSWT+MI  Y +S R  E   LF  +               
Sbjct: 266 CGCIDEARNIFDKIVEKDVVSWTSMIDRYFKSSRWREGFSLFSEL--------------- 325

Query: 244 IQSGNGIYSLSLFNEMRREGIDIVDPLVLSSVVGACASANLALLELGKQIHGLVIALGFE 303
                 + S    NE              + V+ AC  A+L   ELGKQ+HG +  +GF+
Sbjct: 326 ------VGSCERPNE-----------YTFAGVLNAC--ADLTTEELGKQVHGYMTRVGFD 385

Query: 304 SCLFISNALVDMYAKCSDILAAKDIFYRMPRKDVVSWTSIIVGTAQHGKAEEALTLYDEM 363
              F S++LVDMY KC +I +AK +    P+ D+VSWTS+I G AQ+G+ +EAL  +D +
Sbjct: 386 PYSFASSSLVDMYTKCGNIESAKHVVDGCPKPDLVSWTSLIGGCAQNGQPDEALKYFDLL 445

Query: 364 VLSGIKPNEVTFIGLLYACSHVGLVSRGRELFRSMTNDYRINPSLQHYTCLLDLLSRSGH 423
           + SG KP+ VTF+ +L AC+H GLV +G E F S+T  +R++ +  HYTCL+DLL+RSG 
Sbjct: 446 LKSGTKPDHVTFVNVLSACTHAGLVEKGLEFFYSITEKHRLSHTSDHYTCLVDLLARSGR 505

Query: 424 LDEAENLLDEMPFKPDEPTWASLLSACKRHNNLKMGVRIADNVLDLKPEDPSTYILLSNV 483
            ++ ++++ EMP KP +  WAS+L  C  + N+ +    A  +  ++PE+P TY+ ++N+
Sbjct: 506 FEQLKSVISEMPMKPSKFLWASVLGGCSTYGNIDLAEEAAQELFKIEPENPVTYVTMANI 565

Query: 484 YAGAEMWESVSKVRKLMSSMEVRKEPGYSSIDFGKESQVFHAGEGCHPMKDEIFNLLKDL 543
           YA A  WE   K+RK M  + V K PG S  +  ++  VF A +  HPM ++I   L++L
Sbjct: 566 YAAAGKWEEEGKMRKRMQEIGVTKRPGSSWTEIKRKRHVFIAADTSHPMYNQIVEFLREL 625

Query: 544 DAEMRRRGYVPNTSFVLHDMEQQEKEKQLFWHSERLAVAYGLLKAVPGTIIRIVKNLRIC 603
             +M+  GYVP TS VLHD+E ++KE+ L +HSE+LAVA+ +L    GT I++ KNLR C
Sbjct: 626 RKKMKEEGYVPATSLVLHDVEDEQKEENLVYHSEKLAVAFAILSTEEGTAIKVFKNLRSC 685

Query: 604 GDCHNVLKFTSDIKQREIVVRDATRYHHFKEGKCSCNDFW 613
            DCH  +KF S+I +R+I VRD+TR+H F+ G+CSC D+W
Sbjct: 686 VDCHGAIKFISNITKRKITVRDSTRFHCFENGQCSCGDYW 691

BLAST of Lsi03G010590 vs. ExPASy Swiss-Prot
Match: Q9SHZ8 (Pentatricopeptide repeat-containing protein At2g22070 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H41 PE=3 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.3e-127
Identity = 236/623 (37.88%), Postives = 370/623 (59.39%), Query Frame = 0

Query: 24  KLHSQIIKAGFDQSGPLPNTLLDVYGKC-------------------------------G 83
           K+HS I+K G   +  + N+LL++Y KC                               G
Sbjct: 167 KVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVG 226

Query: 84  LIPQALQLFDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIV 143
            +  A+  F++M  RD VTW S+++  NQ     R L + + M     L PD F  A ++
Sbjct: 227 QMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVL 286

Query: 144 RACSSLGYLRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKN-- 203
            AC++L  L +GKQ+H+  + + F    +V ++LI MY++C   + AR + +    K+  
Sbjct: 287 SACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLK 346

Query: 204 SVSWTAMISGYARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRR 263
              +TA++ GY + G   +A  +F  +  R++ +WTA+I G  Q G+   +++LF  M  
Sbjct: 347 IEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVG 406

Query: 264 EGIDIVDPLVLSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSD 323
            G    +   L++++   +S  LA L  GKQIHG  +  G    + +SNAL+ MYAK  +
Sbjct: 407 GG-QRPNSYTLAAMLSVASS--LASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGN 466

Query: 324 ILAAKDIFYRMP-RKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLY 383
           I +A   F  +   +D VSWTS+I+  AQHG AEEAL L++ M++ G++P+ +T++G+  
Sbjct: 467 ITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFS 526

Query: 384 ACSHVGLVSRGRELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDE 443
           AC+H GLV++GR+ F  M +  +I P+L HY C++DL  R+G L EA+  +++MP +PD 
Sbjct: 527 ACTHAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDV 586

Query: 444 PTWASLLSACKRHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLM 503
            TW SLLSAC+ H N+ +G   A+ +L L+PE+   Y  L+N+Y+    WE  +K+RK M
Sbjct: 587 VTWGSLLSACRVHKNIDLGKVAAERLLLLEPENSGAYSALANLYSACGKWEEAAKIRKSM 646

Query: 504 SSMEVRKEPGYSSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMRRRGYVPNTSFVL 563
               V+KE G+S I+   +  VF   +G HP K+EI+  +K +  E+++ GYVP+T+ VL
Sbjct: 647 KDGRVKKEQGFSWIEVKHKVHVFGVEDGTHPEKNEIYMTMKKIWDEIKKMGYVPDTASVL 706

Query: 564 HDMEQQEKEKQLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQRE 613
           HD+E++ KE+ L  HSE+LA+A+GL+     T +RI+KNLR+C DCH  +KF S +  RE
Sbjct: 707 HDLEEEVKEQILRHHSEKLAIAFGLISTPDKTTLRIMKNLRVCNDCHTAIKFISKLVGRE 766

BLAST of Lsi03G010590 vs. ExPASy TrEMBL
Match: A0A1S3BZM7 (LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At4g14050, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103494781 PE=3 SV=1)

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 565/613 (92.17%), Postives = 591/613 (96.41%), Query Frame = 0

Query: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQL 60
           MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFD+S  L NTLLDVYGKCGLIPQALQL
Sbjct: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDKSSLLSNTLLDVYGKCGLIPQALQL 60

Query: 61  FDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGY 120
           FDEMPHRDHV+WASILTAHN+A++PRRTLSMLN MFT DGLQPDHFVFACIVRACSSLGY
Sbjct: 61  FDEMPHRDHVSWASILTAHNKALIPRRTLSMLNIMFTNDGLQPDHFVFACIVRACSSLGY 120

Query: 121 LRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180
           LRLGKQVHARFMLS FC+DEVVKSSLIDMY KC QPDDARAVFDSILFKNSVSWT+MISG
Sbjct: 121 LRLGKQVHARFMLSXFCDDEVVKSSLIDMYTKCGQPDDARAVFDSILFKNSVSWTSMISG 180

Query: 181 YARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLV 240
           YARSGRKCEA++LF +VPVRNLFSWTALISGLIQSG+GI+S SLFNEMRREGIDIVDPLV
Sbjct: 181 YARSGRKCEAMDLFLQVPVRNLFSWTALISGLIQSGHGIFSFSLFNEMRREGIDIVDPLV 240

Query: 241 LSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300
           LSSVVG C  ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR
Sbjct: 241 LSSVVGGC--ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300

Query: 301 MPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRG 360
           MPRKDV+SWTSIIVGTAQHGKAEEALTLYDEMVLS IKPNEVTF+GLLYACSH GLVSRG
Sbjct: 301 MPRKDVISWTSIIVGTAQHGKAEEALTLYDEMVLSRIKPNEVTFVGLLYACSHAGLVSRG 360

Query: 361 RELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACK 420
           RELFRSMT DY INPSLQHYTCLLDLLSRSGHLDEAENLL+E+PFKPDEPTWASLLSACK
Sbjct: 361 RELFRSMTTDYSINPSLQHYTCLLDLLSRSGHLDEAENLLNEIPFKPDEPTWASLLSACK 420

Query: 421 RHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGY 480
           RHNNL+MGVRIAD +LDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLM+SMEVRK+PGY
Sbjct: 421 RHNNLEMGVRIADRLLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMNSMEVRKKPGY 480

Query: 481 SSIDFGKESQVFHAGEGC-HPMKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEK 540
           SSIDFGK+SQVFHAGE C HPMK+EIFNLLKDLDAEMR+RGYVPNTSFVL+D+EQQEKEK
Sbjct: 481 SSIDFGKDSQVFHAGESCDHPMKNEIFNLLKDLDAEMRKRGYVPNTSFVLYDIEQQEKEK 540

Query: 541 QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRYH 600
           QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKF SDI +REIVVRDATRYH
Sbjct: 541 QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFISDIVKREIVVRDATRYH 600

Query: 601 HFKEGKCSCNDFW 613
           HFKEGKCSCNDFW
Sbjct: 601 HFKEGKCSCNDFW 611

BLAST of Lsi03G010590 vs. ExPASy TrEMBL
Match: A0A5D3E178 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00970 PE=3 SV=1)

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 565/613 (92.17%), Postives = 591/613 (96.41%), Query Frame = 0

Query: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQL 60
           MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFD+S  L NTLLDVYGKCGLIPQALQL
Sbjct: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDKSSLLSNTLLDVYGKCGLIPQALQL 60

Query: 61  FDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGY 120
           FDEMPHRDHV+WASILTAHN+A++PRRTLSMLN MFT DGLQPDHFVFACIVRACSSLGY
Sbjct: 61  FDEMPHRDHVSWASILTAHNKALIPRRTLSMLNIMFTNDGLQPDHFVFACIVRACSSLGY 120

Query: 121 LRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180
           LRLGKQVHARFMLS FC+DEVVKSSLIDMY KC QPDDARAVFDSILFKNSVSWT+MISG
Sbjct: 121 LRLGKQVHARFMLSFFCDDEVVKSSLIDMYTKCGQPDDARAVFDSILFKNSVSWTSMISG 180

Query: 181 YARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLV 240
           YARSGRKCEA++LF +VPVRNLFSWTALISGLIQSG+GI+S SLFNEMRREGIDIVDPLV
Sbjct: 181 YARSGRKCEAMDLFLQVPVRNLFSWTALISGLIQSGHGIFSFSLFNEMRREGIDIVDPLV 240

Query: 241 LSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300
           LSSVVG C  ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR
Sbjct: 241 LSSVVGGC--ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300

Query: 301 MPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRG 360
           MPRKDV+SWTSIIVGTAQHGKAEEALTLYDEMVLS IKPNEVTF+GLLYACSH GLVSRG
Sbjct: 301 MPRKDVISWTSIIVGTAQHGKAEEALTLYDEMVLSRIKPNEVTFVGLLYACSHAGLVSRG 360

Query: 361 RELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACK 420
           RELFRSMT DY INPSLQHYTCLLDLLSRSGHLDEAENLL+E+PFKPDEPTWASLLSACK
Sbjct: 361 RELFRSMTTDYSINPSLQHYTCLLDLLSRSGHLDEAENLLNEIPFKPDEPTWASLLSACK 420

Query: 421 RHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGY 480
           RHNNL+MGVRIAD +LDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLM+SMEVRK+PGY
Sbjct: 421 RHNNLEMGVRIADRLLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMNSMEVRKKPGY 480

Query: 481 SSIDFGKESQVFHAGEGC-HPMKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEK 540
           SSIDFGK+SQVFHAGE C HPMK+EIFNLLKDLDAEMR+RGYVPNTSFVL+D+EQQEKEK
Sbjct: 481 SSIDFGKDSQVFHAGESCDHPMKNEIFNLLKDLDAEMRKRGYVPNTSFVLYDIEQQEKEK 540

Query: 541 QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRYH 600
           QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKF SDI +REIVVRDATRYH
Sbjct: 541 QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFISDIVKREIVVRDATRYH 600

Query: 601 HFKEGKCSCNDFW 613
           HFKEGKCSCNDFW
Sbjct: 601 HFKEGKCSCNDFW 611

BLAST of Lsi03G010590 vs. ExPASy TrEMBL
Match: A0A6J1KBS5 (pentatricopeptide repeat-containing protein At4g14050, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111492878 PE=3 SV=1)

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 559/612 (91.34%), Postives = 592/612 (96.73%), Query Frame = 0

Query: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQL 60
           MNI HFLH+LQLCARRQS +AAGKLHSQIIKAG D++GPLPNTLLDVYGKCGLIPQALQL
Sbjct: 1   MNIFHFLHELQLCARRQSTAAAGKLHSQIIKAGLDKAGPLPNTLLDVYGKCGLIPQALQL 60

Query: 61  FDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGY 120
           FDEMPHRDHV+WASILTAHN+AILPRRTLSML+ MFT DGLQPDHFVFACIV AC+SLGY
Sbjct: 61  FDEMPHRDHVSWASILTAHNRAILPRRTLSMLSIMFTHDGLQPDHFVFACIVSACASLGY 120

Query: 121 LRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180
           LRLGKQVHARFMLSSFC+DEVVKSSLIDMYAKC  PDDARAVFDSI FKNSVSWTAMISG
Sbjct: 121 LRLGKQVHARFMLSSFCDDEVVKSSLIDMYAKCGMPDDARAVFDSISFKNSVSWTAMISG 180

Query: 181 YARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLV 240
           YA+SGRK +AIELFQRVPVRNLFSWTALISGLIQSGNGIYS SLFNEMRREGID+VDPLV
Sbjct: 181 YAKSGRKSDAIELFQRVPVRNLFSWTALISGLIQSGNGIYSFSLFNEMRREGIDVVDPLV 240

Query: 241 LSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300
            SSVVGAC  ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFY+
Sbjct: 241 FSSVVGAC--ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYK 300

Query: 301 MPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRG 360
           MPRKDV+SWTSIIVGTAQHGKAEEAL+LYDEMVLSGIKPNEVTF+GLLYACSHVGLV++G
Sbjct: 301 MPRKDVISWTSIIVGTAQHGKAEEALSLYDEMVLSGIKPNEVTFVGLLYACSHVGLVTKG 360

Query: 361 RELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACK 420
           RELFRSMT DY+INPSLQHYTCLLDLLSRSGHL+EAENLL++MPFKPDEPTWASLLSACK
Sbjct: 361 RELFRSMTADYKINPSLQHYTCLLDLLSRSGHLEEAENLLNKMPFKPDEPTWASLLSACK 420

Query: 421 RHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGY 480
           RHNN++MGVRIAD+VLDLKPEDPSTYILLSNVYAGAEMW+SVSKVR+LMS MEVRKEPGY
Sbjct: 421 RHNNMEMGVRIADHVLDLKPEDPSTYILLSNVYAGAEMWDSVSKVRRLMSCMEVRKEPGY 480

Query: 481 SSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEKQ 540
           SSIDFGKESQVFHAGE  HPMK+EIF+LLKDLDA MRRRGYVPNTSFVLHDMEQQEKEKQ
Sbjct: 481 SSIDFGKESQVFHAGETNHPMKEEIFHLLKDLDAGMRRRGYVPNTSFVLHDMEQQEKEKQ 540

Query: 541 LFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRYHH 600
           LFWHSERLA+AYGLLKAVPGT+IRIVKNLRICGDCHNVLKFTSDI +REIVVRDATRYHH
Sbjct: 541 LFWHSERLAMAYGLLKAVPGTVIRIVKNLRICGDCHNVLKFTSDIVKREIVVRDATRYHH 600

Query: 601 FKEGKCSCNDFW 613
           FK+GKCSCNDFW
Sbjct: 601 FKDGKCSCNDFW 610

BLAST of Lsi03G010590 vs. ExPASy TrEMBL
Match: A0A6J1G9N5 (pentatricopeptide repeat-containing protein At4g14050, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111452061 PE=3 SV=1)

HSP 1 Score: 1151.7 bits (2978), Expect = 0.0e+00
Identity = 558/612 (91.18%), Postives = 592/612 (96.73%), Query Frame = 0

Query: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQL 60
           MNI HFLH+LQLCARRQS +AAGKLHSQIIKAG D++GPL NTLLDVYGKCGLIPQALQL
Sbjct: 1   MNIFHFLHELQLCARRQSTAAAGKLHSQIIKAGLDKAGPLANTLLDVYGKCGLIPQALQL 60

Query: 61  FDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGY 120
           FDEMPHRDHV+WASILTAHN+AILPRRTLSML+ MFT DGLQPDHFVFACIV AC+SLGY
Sbjct: 61  FDEMPHRDHVSWASILTAHNRAILPRRTLSMLSIMFTHDGLQPDHFVFACIVSACASLGY 120

Query: 121 LRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180
           LRLGKQVHARFMLSSFC+DEVVKSSLIDMYAKC  PDDARAVFDSILFKNSVSWTAMISG
Sbjct: 121 LRLGKQVHARFMLSSFCDDEVVKSSLIDMYAKCGMPDDARAVFDSILFKNSVSWTAMISG 180

Query: 181 YARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLV 240
           YA+SGRK +AIELFQRVPVRNLFSWTALISGLIQSGNGIYS SLFNEMRREGID+VDPLV
Sbjct: 181 YAKSGRKSDAIELFQRVPVRNLFSWTALISGLIQSGNGIYSFSLFNEMRREGIDVVDPLV 240

Query: 241 LSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300
            SSVVGAC  ANLALLELGKQ+HGLVIA+GFESCLFISN LVDMYAKCSDILAAKDIFY+
Sbjct: 241 FSSVVGAC--ANLALLELGKQMHGLVIAIGFESCLFISNTLVDMYAKCSDILAAKDIFYK 300

Query: 301 MPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRG 360
           MPRKDV+SWTSIIVGTAQHGKAEEAL+LYDEMVLSGIKPNEVTF+GLLYACSHVGLV++G
Sbjct: 301 MPRKDVISWTSIIVGTAQHGKAEEALSLYDEMVLSGIKPNEVTFVGLLYACSHVGLVTKG 360

Query: 361 RELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACK 420
           RELFRSMT DY+INPSLQHYTCLLDLLSRSGHL+EAENLL++MPFKPDEPTWASLLSACK
Sbjct: 361 RELFRSMTADYKINPSLQHYTCLLDLLSRSGHLEEAENLLNKMPFKPDEPTWASLLSACK 420

Query: 421 RHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGY 480
           RHNN++MGVRIAD+VLDLKP+DPSTYILLSNVYAGAEMWESVSKVR+LMS MEVRKEPGY
Sbjct: 421 RHNNMEMGVRIADHVLDLKPKDPSTYILLSNVYAGAEMWESVSKVRRLMSCMEVRKEPGY 480

Query: 481 SSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEKQ 540
           SSIDFGKESQVFHAGE  HPMKDEIF+LLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEKQ
Sbjct: 481 SSIDFGKESQVFHAGETNHPMKDEIFHLLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEKQ 540

Query: 541 LFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRYHH 600
           LFWHSERLA+AYGLLKAVPGT+IRIVKNLRICGDCHNVLKFTSDI +REIVVRDATRYHH
Sbjct: 541 LFWHSERLAMAYGLLKAVPGTVIRIVKNLRICGDCHNVLKFTSDIVKREIVVRDATRYHH 600

Query: 601 FKEGKCSCNDFW 613
           FK+GKCSCNDFW
Sbjct: 601 FKDGKCSCNDFW 610

BLAST of Lsi03G010590 vs. ExPASy TrEMBL
Match: A0A0A0KY72 (DYW_deaminase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001680 PE=3 SV=1)

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 563/613 (91.84%), Postives = 588/613 (95.92%), Query Frame = 0

Query: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQL 60
           MNISHFLHQLQLCARRQSASAAG+LHSQIIKAGFD+S  L NTLLDVYGKCGLIPQALQL
Sbjct: 1   MNISHFLHQLQLCARRQSASAAGELHSQIIKAGFDKSSLLSNTLLDVYGKCGLIPQALQL 60

Query: 61  FDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGY 120
           FDEMP+RDHV+WASILTAHN+A++PRRTLSMLNTMFT DGLQPDHFVFACIVRACSSLGY
Sbjct: 61  FDEMPNRDHVSWASILTAHNKALIPRRTLSMLNTMFTHDGLQPDHFVFACIVRACSSLGY 120

Query: 121 LRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180
           LRLGKQVHARFMLS FC+DEVVKSSLIDMY KC QPDDARAVFDSILFKNSVSWT+MISG
Sbjct: 121 LRLGKQVHARFMLSFFCDDEVVKSSLIDMYTKCGQPDDARAVFDSILFKNSVSWTSMISG 180

Query: 181 YARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLV 240
           YARSGRKCEA++LF + PVRNLFSWTALISGLIQSG+GIYS SLFNEMRREGIDIVDPLV
Sbjct: 181 YARSGRKCEAMDLFLQAPVRNLFSWTALISGLIQSGHGIYSFSLFNEMRREGIDIVDPLV 240

Query: 241 LSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300
           LSSVVG C  ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR
Sbjct: 241 LSSVVGGC--ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300

Query: 301 MPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRG 360
           MPRKDV+SWTSIIVGTAQHGKAEEALTLYDEMVLS IKPNEVTF+GLLYACSH GLVSRG
Sbjct: 301 MPRKDVISWTSIIVGTAQHGKAEEALTLYDEMVLSRIKPNEVTFVGLLYACSHAGLVSRG 360

Query: 361 RELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACK 420
           RELFRSMT DY INPSLQHYTCLLDLLSRSGHLDEAENLLD++PFKPDEPTWASLLSAC 
Sbjct: 361 RELFRSMTTDYSINPSLQHYTCLLDLLSRSGHLDEAENLLDKIPFKPDEPTWASLLSACM 420

Query: 421 RHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGY 480
           RHNNL+MGVRIAD VLDLKPEDPSTYILLSNVYAGAEMW SVSKVRKLMSSMEVRKEPGY
Sbjct: 421 RHNNLEMGVRIADRVLDLKPEDPSTYILLSNVYAGAEMWGSVSKVRKLMSSMEVRKEPGY 480

Query: 481 SSIDFGKESQVFHAGEGC-HPMKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEK 540
           SSIDFGK+SQVFHAGE C HPMK+EI NLLKDLDAEMR+RGYVPNTSFVL+D+EQQEKEK
Sbjct: 481 SSIDFGKDSQVFHAGESCDHPMKNEICNLLKDLDAEMRKRGYVPNTSFVLYDIEQQEKEK 540

Query: 541 QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRYH 600
           QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKF SDI +REI+VRDATRYH
Sbjct: 541 QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFISDIVKREIMVRDATRYH 600

Query: 601 HFKEGKCSCNDFW 613
           HFKEGKCSCNDFW
Sbjct: 601 HFKEGKCSCNDFW 611

BLAST of Lsi03G010590 vs. NCBI nr
Match: XP_038903469.1 (pentatricopeptide repeat-containing protein At4g14050, mitochondrial [Benincasa hispida])

HSP 1 Score: 1196.8 bits (3095), Expect = 0.0e+00
Identity = 587/612 (95.92%), Postives = 600/612 (98.04%), Query Frame = 0

Query: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQL 60
           MNISHFLH+LQLC RRQSASAAGKLHSQIIKAGF++SGPLPNTLLDVYGKCGLIPQALQL
Sbjct: 1   MNISHFLHELQLCTRRQSASAAGKLHSQIIKAGFEKSGPLPNTLLDVYGKCGLIPQALQL 60

Query: 61  FDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGY 120
           FDEMP RDHV+WASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRAC+SLGY
Sbjct: 61  FDEMPQRDHVSWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACASLGY 120

Query: 121 LRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180
           LRLGKQVHARFMLSSFC+DEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG
Sbjct: 121 LRLGKQVHARFMLSSFCDDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180

Query: 181 YARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLV 240
           YARSGRKCEAIELFQRVP+RNLFSWTALISGLIQSGNGIYS SLFNEMRREGIDIVDPLV
Sbjct: 181 YARSGRKCEAIELFQRVPMRNLFSWTALISGLIQSGNGIYSFSLFNEMRREGIDIVDPLV 240

Query: 241 LSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300
           LSSVVGAC  ANLALLELGKQIHGLVIALGFESCL+ISNA VDMYAKCSD+LAAKDIFYR
Sbjct: 241 LSSVVGAC--ANLALLELGKQIHGLVIALGFESCLYISNAFVDMYAKCSDLLAAKDIFYR 300

Query: 301 MPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRG 360
           MPRKDV+SWTSIIVGTAQHGKAEEALTLYDEMVLS +KPNEVTFIGLLYACSHVGLVSRG
Sbjct: 301 MPRKDVISWTSIIVGTAQHGKAEEALTLYDEMVLSRVKPNEVTFIGLLYACSHVGLVSRG 360

Query: 361 RELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACK 420
           RELFRSMT DYRINPSLQHYTCLLDLLSRSGHLDEAENLL+EMPFKPDEPTWASLLSACK
Sbjct: 361 RELFRSMTIDYRINPSLQHYTCLLDLLSRSGHLDEAENLLNEMPFKPDEPTWASLLSACK 420

Query: 421 RHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGY 480
           RHNNLKMGVRIAD+VLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGY
Sbjct: 421 RHNNLKMGVRIADHVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGY 480

Query: 481 SSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEKQ 540
           SSIDFGKESQVFHAGE CH MKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDME QEKEKQ
Sbjct: 481 SSIDFGKESQVFHAGESCHLMKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDMEPQEKEKQ 540

Query: 541 LFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRYHH 600
           LFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKF SDIKQREIVVRDATRYHH
Sbjct: 541 LFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFISDIKQREIVVRDATRYHH 600

Query: 601 FKEGKCSCNDFW 613
           FKEGKCSCNDFW
Sbjct: 601 FKEGKCSCNDFW 610

BLAST of Lsi03G010590 vs. NCBI nr
Match: XP_008454358.1 (PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At4g14050, mitochondrial [Cucumis melo])

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 565/613 (92.17%), Postives = 591/613 (96.41%), Query Frame = 0

Query: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQL 60
           MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFD+S  L NTLLDVYGKCGLIPQALQL
Sbjct: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDKSSLLSNTLLDVYGKCGLIPQALQL 60

Query: 61  FDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGY 120
           FDEMPHRDHV+WASILTAHN+A++PRRTLSMLN MFT DGLQPDHFVFACIVRACSSLGY
Sbjct: 61  FDEMPHRDHVSWASILTAHNKALIPRRTLSMLNIMFTNDGLQPDHFVFACIVRACSSLGY 120

Query: 121 LRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180
           LRLGKQVHARFMLS FC+DEVVKSSLIDMY KC QPDDARAVFDSILFKNSVSWT+MISG
Sbjct: 121 LRLGKQVHARFMLSXFCDDEVVKSSLIDMYTKCGQPDDARAVFDSILFKNSVSWTSMISG 180

Query: 181 YARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLV 240
           YARSGRKCEA++LF +VPVRNLFSWTALISGLIQSG+GI+S SLFNEMRREGIDIVDPLV
Sbjct: 181 YARSGRKCEAMDLFLQVPVRNLFSWTALISGLIQSGHGIFSFSLFNEMRREGIDIVDPLV 240

Query: 241 LSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300
           LSSVVG C  ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR
Sbjct: 241 LSSVVGGC--ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300

Query: 301 MPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRG 360
           MPRKDV+SWTSIIVGTAQHGKAEEALTLYDEMVLS IKPNEVTF+GLLYACSH GLVSRG
Sbjct: 301 MPRKDVISWTSIIVGTAQHGKAEEALTLYDEMVLSRIKPNEVTFVGLLYACSHAGLVSRG 360

Query: 361 RELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACK 420
           RELFRSMT DY INPSLQHYTCLLDLLSRSGHLDEAENLL+E+PFKPDEPTWASLLSACK
Sbjct: 361 RELFRSMTTDYSINPSLQHYTCLLDLLSRSGHLDEAENLLNEIPFKPDEPTWASLLSACK 420

Query: 421 RHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGY 480
           RHNNL+MGVRIAD +LDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLM+SMEVRK+PGY
Sbjct: 421 RHNNLEMGVRIADRLLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMNSMEVRKKPGY 480

Query: 481 SSIDFGKESQVFHAGEGC-HPMKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEK 540
           SSIDFGK+SQVFHAGE C HPMK+EIFNLLKDLDAEMR+RGYVPNTSFVL+D+EQQEKEK
Sbjct: 481 SSIDFGKDSQVFHAGESCDHPMKNEIFNLLKDLDAEMRKRGYVPNTSFVLYDIEQQEKEK 540

Query: 541 QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRYH 600
           QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKF SDI +REIVVRDATRYH
Sbjct: 541 QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFISDIVKREIVVRDATRYH 600

Query: 601 HFKEGKCSCNDFW 613
           HFKEGKCSCNDFW
Sbjct: 601 HFKEGKCSCNDFW 611

BLAST of Lsi03G010590 vs. NCBI nr
Match: KAA0044370.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYK29498.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 565/613 (92.17%), Postives = 591/613 (96.41%), Query Frame = 0

Query: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQL 60
           MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFD+S  L NTLLDVYGKCGLIPQALQL
Sbjct: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDKSSLLSNTLLDVYGKCGLIPQALQL 60

Query: 61  FDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGY 120
           FDEMPHRDHV+WASILTAHN+A++PRRTLSMLN MFT DGLQPDHFVFACIVRACSSLGY
Sbjct: 61  FDEMPHRDHVSWASILTAHNKALIPRRTLSMLNIMFTNDGLQPDHFVFACIVRACSSLGY 120

Query: 121 LRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180
           LRLGKQVHARFMLS FC+DEVVKSSLIDMY KC QPDDARAVFDSILFKNSVSWT+MISG
Sbjct: 121 LRLGKQVHARFMLSFFCDDEVVKSSLIDMYTKCGQPDDARAVFDSILFKNSVSWTSMISG 180

Query: 181 YARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLV 240
           YARSGRKCEA++LF +VPVRNLFSWTALISGLIQSG+GI+S SLFNEMRREGIDIVDPLV
Sbjct: 181 YARSGRKCEAMDLFLQVPVRNLFSWTALISGLIQSGHGIFSFSLFNEMRREGIDIVDPLV 240

Query: 241 LSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300
           LSSVVG C  ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR
Sbjct: 241 LSSVVGGC--ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300

Query: 301 MPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRG 360
           MPRKDV+SWTSIIVGTAQHGKAEEALTLYDEMVLS IKPNEVTF+GLLYACSH GLVSRG
Sbjct: 301 MPRKDVISWTSIIVGTAQHGKAEEALTLYDEMVLSRIKPNEVTFVGLLYACSHAGLVSRG 360

Query: 361 RELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACK 420
           RELFRSMT DY INPSLQHYTCLLDLLSRSGHLDEAENLL+E+PFKPDEPTWASLLSACK
Sbjct: 361 RELFRSMTTDYSINPSLQHYTCLLDLLSRSGHLDEAENLLNEIPFKPDEPTWASLLSACK 420

Query: 421 RHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGY 480
           RHNNL+MGVRIAD +LDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLM+SMEVRK+PGY
Sbjct: 421 RHNNLEMGVRIADRLLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMNSMEVRKKPGY 480

Query: 481 SSIDFGKESQVFHAGEGC-HPMKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEK 540
           SSIDFGK+SQVFHAGE C HPMK+EIFNLLKDLDAEMR+RGYVPNTSFVL+D+EQQEKEK
Sbjct: 481 SSIDFGKDSQVFHAGESCDHPMKNEIFNLLKDLDAEMRKRGYVPNTSFVLYDIEQQEKEK 540

Query: 541 QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRYH 600
           QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKF SDI +REIVVRDATRYH
Sbjct: 541 QLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFISDIVKREIVVRDATRYH 600

Query: 601 HFKEGKCSCNDFW 613
           HFKEGKCSCNDFW
Sbjct: 601 HFKEGKCSCNDFW 611

BLAST of Lsi03G010590 vs. NCBI nr
Match: XP_023523455.1 (pentatricopeptide repeat-containing protein At4g14050, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 558/612 (91.18%), Postives = 592/612 (96.73%), Query Frame = 0

Query: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQL 60
           MNI HFLH+LQLCARRQS +AAGKLHSQIIKAGFD++GPLPNTLLDVYGKCGLIPQALQL
Sbjct: 1   MNIFHFLHELQLCARRQSTAAAGKLHSQIIKAGFDKAGPLPNTLLDVYGKCGLIPQALQL 60

Query: 61  FDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGY 120
           FDEMPHRDHV+WASILTAHN+AILPRRTLSML+ MFT DGLQPDHFVFACIV AC+SLGY
Sbjct: 61  FDEMPHRDHVSWASILTAHNRAILPRRTLSMLSIMFTRDGLQPDHFVFACIVSACASLGY 120

Query: 121 LRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180
           LRLGKQVHARFMLSSFC+DEVVKSSLIDMYAKC  PDDARAVFDSILFKNSVSWTAMISG
Sbjct: 121 LRLGKQVHARFMLSSFCDDEVVKSSLIDMYAKCGMPDDARAVFDSILFKNSVSWTAMISG 180

Query: 181 YARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLV 240
           YA+SGRK +AIELFQRVPVRNLFSWTALISGLIQSGNGIYS SLFNEMRREGID+VDPLV
Sbjct: 181 YAKSGRKSDAIELFQRVPVRNLFSWTALISGLIQSGNGIYSFSLFNEMRREGIDVVDPLV 240

Query: 241 LSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300
            SSVVGAC  ANLALLELGKQ+HGLVIA+GFESCLFISN LVDMYAKCSDILAAKDIFY+
Sbjct: 241 FSSVVGAC--ANLALLELGKQMHGLVIAIGFESCLFISNTLVDMYAKCSDILAAKDIFYK 300

Query: 301 MPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRG 360
           MPRKDV+SWTSIIVGTAQHGKAEEAL+LYDEMVLSGIKPNEVTF+GLLYACSHVGLV++G
Sbjct: 301 MPRKDVISWTSIIVGTAQHGKAEEALSLYDEMVLSGIKPNEVTFVGLLYACSHVGLVTKG 360

Query: 361 RELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACK 420
           RELFRSMT DY+INPSLQHYTCLLDLLSRSGHL+EAENLL++MPFKPDEPTWASLLSACK
Sbjct: 361 RELFRSMTADYKINPSLQHYTCLLDLLSRSGHLEEAENLLNKMPFKPDEPTWASLLSACK 420

Query: 421 RHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGY 480
           RHNN++MGVRIAD+VLDLKPEDPSTY+LLSNVYAGA MWESVSKVR+LMS MEVRKEPGY
Sbjct: 421 RHNNMEMGVRIADHVLDLKPEDPSTYVLLSNVYAGAGMWESVSKVRRLMSCMEVRKEPGY 480

Query: 481 SSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEKQ 540
           SSIDFGKESQVFHAGE  HPMKDEIF+LLKDLDAEMRRRGYVPNTSFVLHDM QQEKEKQ
Sbjct: 481 SSIDFGKESQVFHAGETNHPMKDEIFHLLKDLDAEMRRRGYVPNTSFVLHDMVQQEKEKQ 540

Query: 541 LFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRYHH 600
           LFWHSERLA+AYGLLKAVPGT+IRIVKNLRICGDCHNVLKFTSDI +REIVVRDATRYHH
Sbjct: 541 LFWHSERLAMAYGLLKAVPGTVIRIVKNLRICGDCHNVLKFTSDIVKREIVVRDATRYHH 600

Query: 601 FKEGKCSCNDFW 613
           FK+GKCSCNDFW
Sbjct: 601 FKDGKCSCNDFW 610

BLAST of Lsi03G010590 vs. NCBI nr
Match: XP_022998145.1 (pentatricopeptide repeat-containing protein At4g14050, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 559/612 (91.34%), Postives = 592/612 (96.73%), Query Frame = 0

Query: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQL 60
           MNI HFLH+LQLCARRQS +AAGKLHSQIIKAG D++GPLPNTLLDVYGKCGLIPQALQL
Sbjct: 1   MNIFHFLHELQLCARRQSTAAAGKLHSQIIKAGLDKAGPLPNTLLDVYGKCGLIPQALQL 60

Query: 61  FDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGY 120
           FDEMPHRDHV+WASILTAHN+AILPRRTLSML+ MFT DGLQPDHFVFACIV AC+SLGY
Sbjct: 61  FDEMPHRDHVSWASILTAHNRAILPRRTLSMLSIMFTHDGLQPDHFVFACIVSACASLGY 120

Query: 121 LRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180
           LRLGKQVHARFMLSSFC+DEVVKSSLIDMYAKC  PDDARAVFDSI FKNSVSWTAMISG
Sbjct: 121 LRLGKQVHARFMLSSFCDDEVVKSSLIDMYAKCGMPDDARAVFDSISFKNSVSWTAMISG 180

Query: 181 YARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLV 240
           YA+SGRK +AIELFQRVPVRNLFSWTALISGLIQSGNGIYS SLFNEMRREGID+VDPLV
Sbjct: 181 YAKSGRKSDAIELFQRVPVRNLFSWTALISGLIQSGNGIYSFSLFNEMRREGIDVVDPLV 240

Query: 241 LSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300
            SSVVGAC  ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFY+
Sbjct: 241 FSSVVGAC--ANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYK 300

Query: 301 MPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRG 360
           MPRKDV+SWTSIIVGTAQHGKAEEAL+LYDEMVLSGIKPNEVTF+GLLYACSHVGLV++G
Sbjct: 301 MPRKDVISWTSIIVGTAQHGKAEEALSLYDEMVLSGIKPNEVTFVGLLYACSHVGLVTKG 360

Query: 361 RELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACK 420
           RELFRSMT DY+INPSLQHYTCLLDLLSRSGHL+EAENLL++MPFKPDEPTWASLLSACK
Sbjct: 361 RELFRSMTADYKINPSLQHYTCLLDLLSRSGHLEEAENLLNKMPFKPDEPTWASLLSACK 420

Query: 421 RHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPGY 480
           RHNN++MGVRIAD+VLDLKPEDPSTYILLSNVYAGAEMW+SVSKVR+LMS MEVRKEPGY
Sbjct: 421 RHNNMEMGVRIADHVLDLKPEDPSTYILLSNVYAGAEMWDSVSKVRRLMSCMEVRKEPGY 480

Query: 481 SSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMRRRGYVPNTSFVLHDMEQQEKEKQ 540
           SSIDFGKESQVFHAGE  HPMK+EIF+LLKDLDA MRRRGYVPNTSFVLHDMEQQEKEKQ
Sbjct: 481 SSIDFGKESQVFHAGETNHPMKEEIFHLLKDLDAGMRRRGYVPNTSFVLHDMEQQEKEKQ 540

Query: 541 LFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRYHH 600
           LFWHSERLA+AYGLLKAVPGT+IRIVKNLRICGDCHNVLKFTSDI +REIVVRDATRYHH
Sbjct: 541 LFWHSERLAMAYGLLKAVPGTVIRIVKNLRICGDCHNVLKFTSDIVKREIVVRDATRYHH 600

Query: 601 FKEGKCSCNDFW 613
           FK+GKCSCNDFW
Sbjct: 601 FKDGKCSCNDFW 610

BLAST of Lsi03G010590 vs. TAIR 10
Match: AT4G14050.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 863.2 bits (2229), Expect = 1.2e-250
Identity = 411/614 (66.94%), Postives = 506/614 (82.41%), Query Frame = 0

Query: 1   MNISHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKCGLIPQALQL 60
           M I H+LHQLQLCAR ++ + A  LH+ I+K G  Q  PL NTL++VYGKCG    ALQ+
Sbjct: 1   MLIPHYLHQLQLCARNRTLTTAKALHAHIVKLGIVQCCPLANTLVNVYGKCGAASHALQV 60

Query: 61  FDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIVRACSSLGY 120
           FDEMPHRDH+ WAS+LTA NQA L  +TLS+ +++ +  GL+PD FVF+ +V+AC++LG 
Sbjct: 61  FDEMPHRDHIAWASVLTALNQANLSGKTLSVFSSVGSSSGLRPDDFVFSALVKACANLGS 120

Query: 121 LRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKNSVSWTAMISG 180
           +  G+QVH  F++S +  DEVVKSSL+DMYAKC   + A+AVFDSI  KN++SWTAM+SG
Sbjct: 121 IDHGRQVHCHFIVSEYANDEVVKSSLVDMYAKCGLLNSAKAVFDSIRVKNTISWTAMVSG 180

Query: 181 YARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRREGIDIVDPLV 240
           YA+SGRK EA+ELF+ +PV+NL+SWTALISG +QSG G+ + S+F EMRRE +DI+DPLV
Sbjct: 181 YAKSGRKEEALELFRILPVKNLYSWTALISGFVQSGKGLEAFSVFTEMRRERVDILDPLV 240

Query: 241 LSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSDILAAKDIFYR 300
           LSS+VGAC  ANLA    G+Q+HGLVIALGF+SC+FISNAL+DMYAKCSD++AAKDIF R
Sbjct: 241 LSSIVGAC--ANLAASIAGRQVHGLVIALGFDSCVFISNALIDMYAKCSDVIAAKDIFSR 300

Query: 301 MPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLYACSHVGLVSRG 360
           M  +DVVSWTS+IVG AQHG+AE+AL LYD+MV  G+KPNEVTF+GL+YACSHVG V +G
Sbjct: 301 MRHRDVVSWTSLIVGMAQHGQAEKALALYDDMVSHGVKPNEVTFVGLIYACSHVGFVEKG 360

Query: 361 RELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDEPTWASLLSACK 420
           RELF+SMT DY I PSLQHYTCLLDLL RSG LDEAENL+  MPF PDEPTWA+LLSACK
Sbjct: 361 RELFQSMTKDYGIRPSLQHYTCLLDLLGRSGLLDEAENLIHTMPFPPDEPTWAALLSACK 420

Query: 421 RHNNLKMGVRIADN-VLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLMSSMEVRKEPG 480
           R    +MG+RIAD+ V   K +DPSTYILLSN+YA A +W  VS+ R+ +  MEVRK+PG
Sbjct: 421 RQGRGQMGIRIADHLVSSFKLKDPSTYILLSNIYASASLWGKVSEARRKLGEMEVRKDPG 480

Query: 481 YSSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMR-RRGYVPNTSFVLHDMEQQEKE 540
           +SS++  KE++VF+AGE  HP+K++IF LLK L+ EMR R GYVP+TS++LHDM++QEKE
Sbjct: 481 HSSVEVRKETEVFYAGETSHPLKEDIFRLLKKLEEEMRIRNGYVPDTSWILHDMDEQEKE 540

Query: 541 KQLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQREIVVRDATRY 600
           K LFWHSER AVAYGLLKAVPGT IRIVKNLR+CGDCH VLK  S+I +REI+VRDATRY
Sbjct: 541 KLLFWHSERSAVAYGLLKAVPGTPIRIVKNLRVCGDCHVVLKHISEITEREIIVRDATRY 600

Query: 601 HHFKEGKCSCNDFW 613
           HHFK GKCSCNDFW
Sbjct: 601 HHFKGGKCSCNDFW 612

BLAST of Lsi03G010590 vs. TAIR 10
Match: AT1G25360.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 482.6 bits (1241), Expect = 4.6e-136
Identity = 251/626 (40.10%), Postives = 368/626 (58.79%), Query Frame = 0

Query: 24  KLHSQIIKAGFDQSGPLPNTLLDVYGKC----GLIPQALQLFDEMPHRDHVTWASILT-- 83
           + H+  +K+G      + N L+ VY KC     L+  A ++FDE+  +D  +W +++T  
Sbjct: 170 QFHAAALKSGAGYITSVSNALVSVYSKCASSPSLLHSARKVFDEILEKDERSWTTMMTGY 229

Query: 84  -----------------------AHNQAI-------LPRRTLSMLNTMFTLDGLQPDHFV 143
                                  A+N  I         +  L M+  M +  G++ D F 
Sbjct: 230 VKNGYFDLGEELLEGMDDNMKLVAYNAMISGYVNRGFYQEALEMVRRMVS-SGIELDEFT 289

Query: 144 FACIVRACSSLGYLRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSIL 203
           +  ++RAC++ G L+LGKQVHA ++L          +SL+ +Y KC + D+ARA+F+ + 
Sbjct: 290 YPSVIRACATAGLLQLGKQVHA-YVLRREDFSFHFDNSLVSLYYKCGKFDEARAIFEKMP 349

Query: 204 FKNSVSWTAMISGYARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNE 263
            K+ VSW A++SGY  SG   EA  +F+ +  +N+ SW  +ISGL ++G G   L LF+ 
Sbjct: 350 AKDLVSWNALLSGYVSSGHIGEAKLIFKEMKEKNILSWMIMISGLAENGFGEEGLKLFSC 409

Query: 264 MRREGIDIVDPLVLSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAK 323
           M+REG +  D    S  + +CA   L     G+Q H  ++ +GF+S L   NAL+ MYAK
Sbjct: 410 MKREGFEPCD-YAFSGAIKSCAV--LGAYCNGQQYHAQLLKIGFDSSLSAGNALITMYAK 469

Query: 324 CSDILAAKDIFYRMPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGL 383
           C  +  A+ +F  MP  D VSW ++I    QHG   EA+ +Y+EM+  GI+P+ +T + +
Sbjct: 470 CGVVEEARQVFRTMPCLDSVSWNALIAALGQHGHGAEAVDVYEEMLKKGIRPDRITLLTV 529

Query: 384 LYACSHVGLVSRGRELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKP 443
           L ACSH GLV +GR+ F SM   YRI P   HY  L+DLL RSG   +AE++++ +PFKP
Sbjct: 530 LTACSHAGLVDQGRKYFDSMETVYRIPPGADHYARLIDLLCRSGKFSDAESVIESLPFKP 589

Query: 444 DEPTWASLLSACKRHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRK 503
               W +LLS C+ H N+++G+  AD +  L PE   TY+LLSN++A    WE V++VRK
Sbjct: 590 TAEIWEALLSGCRVHGNMELGIIAADKLFGLIPEHDGTYMLLSNMHAATGQWEEVARVRK 649

Query: 504 LMSSMEVRKEPGYSSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMRRRGYVPNTSF 563
           LM    V+KE   S I+   +   F   +  HP  + ++  L+DL  EMRR GYVP+TSF
Sbjct: 650 LMRDRGVKKEVACSWIEMETQVHTFLVDDTSHPEAEAVYIYLQDLGKEMRRLGYVPDTSF 709

Query: 564 VLHDMEQQ-EKEKQLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIK 613
           VLHD+E    KE  L  HSE++AVA+GL+K  PGT IRI KNLR CGDCHN  +F S + 
Sbjct: 710 VLHDVESDGHKEDMLTTHSEKIAVAFGLMKLPPGTTIRIFKNLRTCGDCHNFFRFLSWVV 769

BLAST of Lsi03G010590 vs. TAIR 10
Match: AT4G02750.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 464.2 bits (1193), Expect = 1.7e-130
Identity = 234/572 (40.91%), Postives = 351/572 (61.36%), Query Frame = 0

Query: 42  NTLLDVYGKCGLIPQALQLFDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGL 101
           N LL  + K   I +A Q FD M  RD V+W +I+T + Q+      +     +F    +
Sbjct: 223 NCLLGGFVKKKKIVEARQFFDSMNVRDVVSWNTIITGYAQS----GKIDEARQLFDESPV 282

Query: 102 QPDHFVFACIVRACSSLGYLRLGKQVHARFMLSSFCE-DEVVKSSLIDMYAKCAQPDDAR 161
           Q D F +  +V      GY++      AR +     E +EV  ++++  Y +  + + A+
Sbjct: 283 Q-DVFTWTAMVS-----GYIQNRMVEEARELFDKMPERNEVSWNAMLAGYVQGERMEMAK 342

Query: 162 AVFDSILFKNSVSWTAMISGYARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIY 221
            +FD +  +N  +W  MI+GYA+ G+  EA  LF ++P R+  SW A+I+G  QSG+   
Sbjct: 343 ELFDVMPCRNVSTWNTMITGYAQCGKISEAKNLFDKMPKRDPVSWAAMIAGYSQSGHSFE 402

Query: 222 SLSLFNEMRREGIDIVDPLVLSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNA 281
           +L LF +M REG   ++    SS +  C  A++  LELGKQ+HG ++  G+E+  F+ NA
Sbjct: 403 ALRLFVQMEREG-GRLNRSSFSSALSTC--ADVVALELGKQLHGRLVKGGYETGCFVGNA 462

Query: 282 LVDMYAKCSDILAAKDIFYRMPRKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPN 341
           L+ MY KC  I  A D+F  M  KD+VSW ++I G ++HG  E AL  ++ M   G+KP+
Sbjct: 463 LLLMYCKCGSIEEANDLFKEMAGKDIVSWNTMIAGYSRHGFGEVALRFFESMKREGLKPD 522

Query: 342 EVTFIGLLYACSHVGLVSRGRELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLL 401
           + T + +L ACSH GLV +GR+ F +MT DY + P+ QHY C++DLL R+G L++A NL+
Sbjct: 523 DATMVAVLSACSHTGLVDKGRQYFYTMTQDYGVMPNSQHYACMVDLLGRAGLLEDAHNLM 582

Query: 402 DEMPFKPDEPTWASLLSACKRHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWE 461
             MPF+PD   W +LL A + H N ++    AD +  ++PE+   Y+LLSN+YA +  W 
Sbjct: 583 KNMPFEPDAAIWGTLLGASRVHGNTELAETAADKIFAMEPENSGMYVLLSNLYASSGRWG 642

Query: 462 SVSKVRKLMSSMEVRKEPGYSSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMRRRG 521
            V K+R  M    V+K PGYS I+   ++  F  G+  HP KDEIF  L++LD  M++ G
Sbjct: 643 DVGKLRVRMRDKGVKKVPGYSWIEIQNKTHTFSVGDEFHPEKDEIFAFLEELDLRMKKAG 702

Query: 522 YVPNTSFVLHDMEQQEKEKQLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLK 581
           YV  TS VLHD+E++EKE+ + +HSERLAVAYG+++   G  IR++KNLR+C DCHN +K
Sbjct: 703 YVSKTSVVLHDVEEEEKERMVRYHSERLAVAYGIMRVSSGRPIRVIKNLRVCEDCHNAIK 762

Query: 582 FTSDIKQREIVVRDATRYHHFKEGKCSCNDFW 613
           + + I  R I++RD  R+HHFK+G CSC D+W
Sbjct: 763 YMARITGRLIILRDNNRFHHFKDGSCSCGDYW 781

BLAST of Lsi03G010590 vs. TAIR 10
Match: AT4G37170.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 461.1 bits (1185), Expect = 1.4e-129
Identity = 236/640 (36.88%), Postives = 365/640 (57.03%), Query Frame = 0

Query: 4   SHFLHQLQLCARRQSASAAGKLHSQIIKAGFDQSGPLPNTLLDVYGKC------------ 63
           S + + +Q+C++ ++     K+H  I  +GF     + N LL +Y KC            
Sbjct: 86  STYCNLIQVCSQTRALEEGKKVHEHIRTSGFVPGIVIWNRLLRMYAKCGSLVDARKVFDE 145

Query: 64  -------------------GLIPQALQLFDEMPHRDHVTWASILTAHNQAILPRRTLSML 123
                              GL+ +A +LFDEM  +D  +W +++T + +   P   L + 
Sbjct: 146 MPNRDLCSWNVMVNGYAEVGLLEEARKLFDEMTEKDSYSWTAMVTGYVKKDQPEEALVLY 205

Query: 124 NTMFTLDGLQPDHFVFACIVRACSSLGYLRLGKQVHARFMLSSFCEDEVVKSSLIDMYAK 183
           + M  +   +P+ F  +  V A +++  +R GK++H   + +    DEV+ SSL+DMY K
Sbjct: 206 SLMQRVPNSRPNIFTVSIAVAAAAAVKCIRRGKEIHGHIVRAGLDSDEVLWSSLMDMYGK 265

Query: 184 CAQPDDARAVFDSILFKNSVSWTAMISGYARSGRKCEAIELFQRVPVRNLFSWTALISGL 243
           C   D+AR +FD I+ K+ VSWT+MI  Y +S R  E   LF  +               
Sbjct: 266 CGCIDEARNIFDKIVEKDVVSWTSMIDRYFKSSRWREGFSLFSEL--------------- 325

Query: 244 IQSGNGIYSLSLFNEMRREGIDIVDPLVLSSVVGACASANLALLELGKQIHGLVIALGFE 303
                 + S    NE              + V+ AC  A+L   ELGKQ+HG +  +GF+
Sbjct: 326 ------VGSCERPNE-----------YTFAGVLNAC--ADLTTEELGKQVHGYMTRVGFD 385

Query: 304 SCLFISNALVDMYAKCSDILAAKDIFYRMPRKDVVSWTSIIVGTAQHGKAEEALTLYDEM 363
              F S++LVDMY KC +I +AK +    P+ D+VSWTS+I G AQ+G+ +EAL  +D +
Sbjct: 386 PYSFASSSLVDMYTKCGNIESAKHVVDGCPKPDLVSWTSLIGGCAQNGQPDEALKYFDLL 445

Query: 364 VLSGIKPNEVTFIGLLYACSHVGLVSRGRELFRSMTNDYRINPSLQHYTCLLDLLSRSGH 423
           + SG KP+ VTF+ +L AC+H GLV +G E F S+T  +R++ +  HYTCL+DLL+RSG 
Sbjct: 446 LKSGTKPDHVTFVNVLSACTHAGLVEKGLEFFYSITEKHRLSHTSDHYTCLVDLLARSGR 505

Query: 424 LDEAENLLDEMPFKPDEPTWASLLSACKRHNNLKMGVRIADNVLDLKPEDPSTYILLSNV 483
            ++ ++++ EMP KP +  WAS+L  C  + N+ +    A  +  ++PE+P TY+ ++N+
Sbjct: 506 FEQLKSVISEMPMKPSKFLWASVLGGCSTYGNIDLAEEAAQELFKIEPENPVTYVTMANI 565

Query: 484 YAGAEMWESVSKVRKLMSSMEVRKEPGYSSIDFGKESQVFHAGEGCHPMKDEIFNLLKDL 543
           YA A  WE   K+RK M  + V K PG S  +  ++  VF A +  HPM ++I   L++L
Sbjct: 566 YAAAGKWEEEGKMRKRMQEIGVTKRPGSSWTEIKRKRHVFIAADTSHPMYNQIVEFLREL 625

Query: 544 DAEMRRRGYVPNTSFVLHDMEQQEKEKQLFWHSERLAVAYGLLKAVPGTIIRIVKNLRIC 603
             +M+  GYVP TS VLHD+E ++KE+ L +HSE+LAVA+ +L    GT I++ KNLR C
Sbjct: 626 RKKMKEEGYVPATSLVLHDVEDEQKEENLVYHSEKLAVAFAILSTEEGTAIKVFKNLRSC 685

Query: 604 GDCHNVLKFTSDIKQREIVVRDATRYHHFKEGKCSCNDFW 613
            DCH  +KF S+I +R+I VRD+TR+H F+ G+CSC D+W
Sbjct: 686 VDCHGAIKFISNITKRKITVRDSTRFHCFENGQCSCGDYW 691

BLAST of Lsi03G010590 vs. TAIR 10
Match: AT2G22070.1 (pentatricopeptide (PPR) repeat-containing protein )

HSP 1 Score: 458.4 bits (1178), Expect = 9.2e-129
Identity = 236/623 (37.88%), Postives = 370/623 (59.39%), Query Frame = 0

Query: 24  KLHSQIIKAGFDQSGPLPNTLLDVYGKC-------------------------------G 83
           K+HS I+K G   +  + N+LL++Y KC                               G
Sbjct: 167 KVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVG 226

Query: 84  LIPQALQLFDEMPHRDHVTWASILTAHNQAILPRRTLSMLNTMFTLDGLQPDHFVFACIV 143
            +  A+  F++M  RD VTW S+++  NQ     R L + + M     L PD F  A ++
Sbjct: 227 QMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVL 286

Query: 144 RACSSLGYLRLGKQVHARFMLSSFCEDEVVKSSLIDMYAKCAQPDDARAVFDSILFKN-- 203
            AC++L  L +GKQ+H+  + + F    +V ++LI MY++C   + AR + +    K+  
Sbjct: 287 SACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLK 346

Query: 204 SVSWTAMISGYARSGRKCEAIELFQRVPVRNLFSWTALISGLIQSGNGIYSLSLFNEMRR 263
              +TA++ GY + G   +A  +F  +  R++ +WTA+I G  Q G+   +++LF  M  
Sbjct: 347 IEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVG 406

Query: 264 EGIDIVDPLVLSSVVGACASANLALLELGKQIHGLVIALGFESCLFISNALVDMYAKCSD 323
            G    +   L++++   +S  LA L  GKQIHG  +  G    + +SNAL+ MYAK  +
Sbjct: 407 GG-QRPNSYTLAAMLSVASS--LASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGN 466

Query: 324 ILAAKDIFYRMP-RKDVVSWTSIIVGTAQHGKAEEALTLYDEMVLSGIKPNEVTFIGLLY 383
           I +A   F  +   +D VSWTS+I+  AQHG AEEAL L++ M++ G++P+ +T++G+  
Sbjct: 467 ITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFS 526

Query: 384 ACSHVGLVSRGRELFRSMTNDYRINPSLQHYTCLLDLLSRSGHLDEAENLLDEMPFKPDE 443
           AC+H GLV++GR+ F  M +  +I P+L HY C++DL  R+G L EA+  +++MP +PD 
Sbjct: 527 ACTHAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDV 586

Query: 444 PTWASLLSACKRHNNLKMGVRIADNVLDLKPEDPSTYILLSNVYAGAEMWESVSKVRKLM 503
            TW SLLSAC+ H N+ +G   A+ +L L+PE+   Y  L+N+Y+    WE  +K+RK M
Sbjct: 587 VTWGSLLSACRVHKNIDLGKVAAERLLLLEPENSGAYSALANLYSACGKWEEAAKIRKSM 646

Query: 504 SSMEVRKEPGYSSIDFGKESQVFHAGEGCHPMKDEIFNLLKDLDAEMRRRGYVPNTSFVL 563
               V+KE G+S I+   +  VF   +G HP K+EI+  +K +  E+++ GYVP+T+ VL
Sbjct: 647 KDGRVKKEQGFSWIEVKHKVHVFGVEDGTHPEKNEIYMTMKKIWDEIKKMGYVPDTASVL 706

Query: 564 HDMEQQEKEKQLFWHSERLAVAYGLLKAVPGTIIRIVKNLRICGDCHNVLKFTSDIKQRE 613
           HD+E++ KE+ L  HSE+LA+A+GL+     T +RI+KNLR+C DCH  +KF S +  RE
Sbjct: 707 HDLEEEVKEQILRHHSEKLAIAFGLISTPDKTTLRIMKNLRVCNDCHTAIKFISKLVGRE 766

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O232661.7e-24966.94Pentatricopeptide repeat-containing protein At4g14050, mitochondrial OS=Arabidop... [more]
Q9FRI56.4e-13540.10Pentatricopeptide repeat-containing protein At1g25360 OS=Arabidopsis thaliana OX... [more]
Q9SY022.4e-12940.91Pentatricopeptide repeat-containing protein At4g02750 OS=Arabidopsis thaliana OX... [more]
O231692.0e-12836.88Pentatricopeptide repeat-containing protein At4g37170 OS=Arabidopsis thaliana OX... [more]
Q9SHZ81.3e-12737.88Pentatricopeptide repeat-containing protein At2g22070 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A1S3BZM70.0e+0092.17LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At4g14050, mito... [more]
A0A5D3E1780.0e+0092.17Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1KBS50.0e+0091.34pentatricopeptide repeat-containing protein At4g14050, mitochondrial OS=Cucurbit... [more]
A0A6J1G9N50.0e+0091.18pentatricopeptide repeat-containing protein At4g14050, mitochondrial OS=Cucurbit... [more]
A0A0A0KY720.0e+0091.84DYW_deaminase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G0016... [more]
Match NameE-valueIdentityDescription
XP_038903469.10.0e+0095.92pentatricopeptide repeat-containing protein At4g14050, mitochondrial [Benincasa ... [more]
XP_008454358.10.0e+0092.17PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At4g... [more]
KAA0044370.10.0e+0092.17pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYK29498... [more]
XP_023523455.10.0e+0091.18pentatricopeptide repeat-containing protein At4g14050, mitochondrial [Cucurbita ... [more]
XP_022998145.10.0e+0091.34pentatricopeptide repeat-containing protein At4g14050, mitochondrial [Cucurbita ... [more]
Match NameE-valueIdentityDescription
AT4G14050.11.2e-25066.94Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G25360.14.6e-13640.10Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G02750.11.7e-13040.91Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G37170.11.4e-12936.88Pentatricopeptide repeat (PPR) superfamily protein [more]
AT2G22070.19.2e-12937.88pentatricopeptide (PPR) repeat-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 304..351
e-value: 4.7E-11
score: 42.7
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 380..403
e-value: 3.2E-4
score: 20.8
coord: 42..67
e-value: 2.2E-4
score: 21.3
coord: 203..233
e-value: 4.4E-6
score: 26.6
coord: 172..197
e-value: 3.1E-4
score: 20.8
coord: 144..166
e-value: 0.6
score: 10.5
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 380..404
e-value: 8.4E-4
score: 17.3
coord: 203..233
e-value: 4.5E-4
score: 18.2
coord: 42..70
e-value: 8.0E-4
score: 17.4
coord: 307..341
e-value: 6.8E-7
score: 27.1
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 305..339
score: 12.057487
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 170..204
score: 10.128299
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 376..410
score: 9.569272
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 371..437
e-value: 1.2E-6
score: 30.1
coord: 166..230
e-value: 4.5E-6
score: 28.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 242..370
e-value: 3.1E-25
score: 91.2
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 1..165
e-value: 1.0E-21
score: 79.6
IPR032867DYW domainPFAMPF14432DYW_deaminasecoord: 479..602
e-value: 2.0E-35
score: 121.4
NoneNo IPR availablePANTHERPTHR47928:SF153OS11G0661000 PROTEINcoord: 5..600
NoneNo IPR availablePANTHERPTHR47928REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 5..600

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G010590.1Lsi03G010590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016554 cytidine to uridine editing
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding