Lsi03G010570 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G010570
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Alkylated DNA repair protein alkB-like protein 8
Location	chr03: 20307473 .. 20309951 (-)
RNA-Seq Expression	Lsi03G010570
Synteny	Lsi03G010570

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTATTTTCAACAAAAATACCAAATAACTCTTTGTTGACGTCAAGTTCATGAACTCGCGCACATGGAAAGCTCATCTTACTCTCGCTTCCTCTCTCTCTCCTCTCGCTCTCAGTGATGATCCATATGGGTCGTCTTCTTCCCTTTTCTCGCTCCGCCAGTGCTCGGTTGTCGAGTGGAAGGGTGGAAATTTGTCGAGAAAAAGAAAAAACTGGACGGAAGCAAGGCCCGGGGGAGTGGAGGGAAATGGGGGAAAAACTGGACGATGAAGACGAAATCTTGAAGCAAGTATTTGGCGATTCTTCGGGAGACGAAGGCTTCGAAGATCAAACTGTAATGAATGATTTATCGTATGAAGTGGGTCATATTCACAAATGGGAGCAGGTCAAGGAAATCAAAGGGTTGTGGCTGTGCAGAATCTTCCTCTCGCCCCAACACCAATCATCACTCCTCTCCGCAATCCGAAACGGTTCGTAATTTCTTATCTCAAGTTGTTCCGTAACATGAAACTCATCTTCGTTCTGTTTTCGAATTTGATCCCTGAACTTAAGATTTCATTGAATGAACCATAGGCTTACGTTTTACAATTTCAATATGTTTAGCGTACCCTCTTTATGAGATTGTAGATACTTGAATCATGTTTGGAAATGTTCATGAAACTGTGCCCATAAAGTCATGCTAATCTTATACTCTTATGTTTTCAGAAGGGTGGTTCATGGAAGCCTCTCAAAATCAGGTACCTTCAGTTTTTAATCTTGATAAGCTTATTATTAGGGGTCTATTATATCTGTTGTTATCCCCAGAAACCAATTCTTCACTGTTACTGAGCAAGCACTGAGGATTTTAAATGCTGTAGTGTTGTTGGATTTTGTTCTTGAAGTTTAGACTAGGATAATATTCTTAACTACTGTTACTTTTTCTCTTCAGGCCATGAGGTTTGGAAACCTTCCAACATGGGCTGTTGAACTTTCAGATTCTGTTTATGAGGCAGTGCTTTCAAGTAACCACATGACAGATACTTTGATTGTGGATCGCTATAATGGAGATAAGGTTGATTGTCCTCTTCCATCAGACATTTTGTGGAGAGAACCACTCTTTGATCAAATGATAGCTAATGTTTATCAACCAGGTGAGGTGCGACGATGACCCATTTCTATTCTATTGTGATTAGACATCTGTTTGAACCGAACCTCCAGGACGATAGCTTCACAGGCACTAGGGGCTGTTTGGAAACAGTTTTTTTTGATGTAGAACTAGATCATCTTTTTGTTATAGTTGAATGTAGTTTAAGAGCCAGTTTGCAAAACAGTTTCTGAAAACAGAATTTGGAGAATAACCCTGTAGCCACTAAGAATCCAAGATTGTGATGTTTGGTCTGTCAAGATGAAGGGTGTACTTTAACAAGGAATAAAAACAGTTTTGTATTTTTATATTTTATTTGAGAGTTTAACAATAGGTGGGAGTGAGAGTGGGGGAATTTGAACGTTTGACCTCTTAGTTTTAGTACAAGAACCATGGTATAAGAGATCGAACCCTCGACCTCTAGGGAGTAATGTCATGTCATTTACCGTTGAGCTAAGCTTGCTTTGACGTTTTACCTCTTAGTTGAGTGCGTCTGTCTTAGTTAGTTGAGTTGTGCTGGGGTTAACAAAAAAGAAAGAGTATCTTGTGGTGTAGTATTTTGATTCATTCATTTCATATCTATGTTATGAATTGTGTTTATAATATTTCATTTGGATTATTTGACCACGTTGTGGACACTTCAACTGCAATCATGAGTAGATAGGAAGCCTTAATATTCTGCTAATGTGATAAAAATGATTTGTGAATGGCATGTTTTTAAAGCACTCTGGGCCATGTTGTTTGAGAAACTTTATATCTGACGTTGCTTGCCTTTTGTTTATCTTCATTAGCATTACATTACAATTCATCTTAGCTTTCCTCATTAGTTATTGATTCACGACATTTTTATAACTTCCCTCTAATATTATCTATAAAGGGAATCTGTGCTCATGTTGACCTTATGCGTTTCGAAGATGGAATTGCCATTGTCTCCCTCGAGTCACCATGTGTGATGCATTTTACCCGAGTTGATGAAACTTCCTGTGATCCTTCAATCAAGGGAGAAGTCGATTTATCGGCATCGAAAGTGCCCGTATATCTAAACCCAGGATCTCTTGTTATATTGTGGGGCGAAGCCCGCTATCTCTGGAAGCATGAGATAAATCGTAAGCCCGGGTTTCAAATATGGGAAGGCCAGGAACTTACTCAGGGAAGGCGAACTTCCATTACACTGAGAAAGCTCTGTCATGTTGAATAATGGGAGCTGCATTTTGTTCCATGTTCATACTTTGTTGTGTTTAACTGAATGAACCATTGGCTGTTATTTGGCTAGGATATCTTCAAGTCATTGTTCTAGATCTTGAAAAGGAGAAATCAGACAAGGATGCAGATCTTTCTATACATGGAGCAGTTCCATGA

mRNA sequence

TGTATTTTCAACAAAAATACCAAATAACTCTTTGTTGACGTCAAGTTCATGAACTCGCGCACATGGAAAGCTCATCTTACTCTCGCTTCCTCTCTCTCTCCTCTCGCTCTCAGTGATGATCCATATGGGTCGTCTTCTTCCCTTTTCTCGCTCCGCCAGTGCTCGGTTGTCGAGTGGAAGGGTGGAAATTTGTCGAGAAAAAGAAAAAACTGGACGGAAGCAAGGCCCGGGGGAGTGGAGGGAAATGGGGGAAAAACTGGACGATGAAGACGAAATCTTGAAGCAAGTATTTGGCGATTCTTCGGGAGACGAAGGCTTCGAAGATCAAACTGTAATGAATGATTTATCGTATGAAGTGGGTCATATTCACAAATGGGAGCAGGTCAAGGAAATCAAAGGGTTGTGGCTGTGCAGAATCTTCCTCTCGCCCCAACACCAATCATCACTCCTCTCCGCAATCCGAAACGAAGGGTGGTTCATGGAAGCCTCTCAAAATCAGGCCATGAGGTTTGGAAACCTTCCAACATGGGCTGTTGAACTTTCAGATTCTGTTTATGAGGCAGTGCTTTCAAGTAACCACATGACAGATACTTTGATTGTGGATCGCTATAATGGAGATAAGGTTGATTGTCCTCTTCCATCAGACATTTTGTGGAGAGAACCACTCTTTGATCAAATGATAGCTAATGTTTATCAACCAGGTGAGGGAATCTGTGCTCATGTTGACCTTATGCGTTTCGAAGATGGAATTGCCATTGTCTCCCTCGAGTCACCATGTGTGATGCATTTTACCCGAGTTGATGAAACTTCCTGTGATCCTTCAATCAAGGGAGAAGTCGATTTATCGGCATCGAAAGTGCCCGTATATCTAAACCCAGGATCTCTTGTTATATTGTGGGGCGAAGCCCGCTATCTCTGGAAGCATGAGATAAATCGATATCTTCAAGTCATTGTTCTAGATCTTGAAAAGGAGAAATCAGACAAGGATGCAGATCTTTCTATACATGGAGCAGTTCCATGA

Coding sequence (CDS)

ATGATCCATATGGGTCGTCTTCTTCCCTTTTCTCGCTCCGCCAGTGCTCGGTTGTCGAGTGGAAGGGTGGAAATTTGTCGAGAAAAAGAAAAAACTGGACGGAAGCAAGGCCCGGGGGAGTGGAGGGAAATGGGGGAAAAACTGGACGATGAAGACGAAATCTTGAAGCAAGTATTTGGCGATTCTTCGGGAGACGAAGGCTTCGAAGATCAAACTGTAATGAATGATTTATCGTATGAAGTGGGTCATATTCACAAATGGGAGCAGGTCAAGGAAATCAAAGGGTTGTGGCTGTGCAGAATCTTCCTCTCGCCCCAACACCAATCATCACTCCTCTCCGCAATCCGAAACGAAGGGTGGTTCATGGAAGCCTCTCAAAATCAGGCCATGAGGTTTGGAAACCTTCCAACATGGGCTGTTGAACTTTCAGATTCTGTTTATGAGGCAGTGCTTTCAAGTAACCACATGACAGATACTTTGATTGTGGATCGCTATAATGGAGATAAGGTTGATTGTCCTCTTCCATCAGACATTTTGTGGAGAGAACCACTCTTTGATCAAATGATAGCTAATGTTTATCAACCAGGTGAGGGAATCTGTGCTCATGTTGACCTTATGCGTTTCGAAGATGGAATTGCCATTGTCTCCCTCGAGTCACCATGTGTGATGCATTTTACCCGAGTTGATGAAACTTCCTGTGATCCTTCAATCAAGGGAGAAGTCGATTTATCGGCATCGAAAGTGCCCGTATATCTAAACCCAGGATCTCTTGTTATATTGTGGGGCGAAGCCCGCTATCTCTGGAAGCATGAGATAAATCGATATCTTCAAGTCATTGTTCTAGATCTTGAAAAGGAGAAATCAGACAAGGATGCAGATCTTTCTATACATGGAGCAGTTCCATGA

Protein sequence

MIHMGRLLPFSRSASARLSSGRVEICREKEKTGRKQGPGEWREMGEKLDDEDEILKQVFGDSSGDEGFEDQTVMNDLSYEVGHIHKWEQVKEIKGLWLCRIFLSPQHQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVDRYNGDKVDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVMHFTRVDETSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEINRYLQVIVLDLEKEKSDKDADLSIHGAVP

Homology

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BLAST of Lsi03G010570 vs. ExPASy Swiss-Prot
Match: Q8RWY1 (Alkylated DNA repair protein ALKBH8 homolog OS=Arabidopsis thaliana OX=3702 GN=ALKBH8 PE=2 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 1.6e-09
Identity = 36/91 (39.56%), Postives = 48/91 (52.75%), Query Frame = 0


Query: 186 DQMIANVYQPGEGICAHVDL-MRFEDGIAIVSLESPCVMHFTRVDETSCDPS---IKGEV 245
           DQ+  N Y  G G+  H+D    FED I  +SL  PC+M F R   ++   S    +   
Sbjct: 210 DQLTVNEYPSGVGLSPHIDTHSAFEDCIFSLSLAGPCIMEFRRYSVSTWKASTTDAEKSG 269

Query: 246 DLSASKVPVYLNPGSLVILWGEARYLWKHEI 273
           D S  K  +YL P S+++L GEARY W H I
Sbjct: 270 DSSCIKKALYLPPRSMLLLSGEARYAWNHYI 300

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BLAST of Lsi03G010570 vs. ExPASy Swiss-Prot
Match: Q9UT12 (Uncharacterized protein P8A3.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP8A3.02c PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.0e-09
Identity = 40/102 (39.22%), Postives = 51/102 (50.00%), Query Frame = 0


Query: 174 LPSDI---LWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVMHFTRVDE 233
           +PSD    LW+    + +I  VY PG+GI  H DL  F DG+AI S  S   M FT    
Sbjct: 101 IPSDFKQRLWKGQDAEAIIMQVYNPGDGIIPHKDLEMFGDGVAIFSFLSNTTMIFTH--- 160

Query: 234 TSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEI 273
               P +K        K  + L  GSL+++ G ARY W HEI
Sbjct: 161 ----PELK-------LKSKIRLEKGSLLLMSGTARYDWFHEI 188

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BLAST of Lsi03G010570 vs. ExPASy Swiss-Prot
Match: Q95K79 (Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.7e-09
Identity = 38/88 (43.18%), Postives = 47/88 (53.41%), Query Frame = 0


Query: 186 DQMIANVYQPGEGICAHVDL-MRFEDGIAIVSLESPCVMHFTRVDETSCDPSIKGEVDLS 245
           DQM  N Y+PG+GI AH+D    FED I  +SL S  VM F   D T+            
Sbjct: 222 DQMTINQYEPGQGIPAHIDTHSAFEDEIVSLSLGSEIVMDFKHPDGTA------------ 281

Query: 246 ASKVPVYLNPGSLVILWGEARYLWKHEI 273
              VPV L   SL+++ GE+RYLW H I
Sbjct: 282 ---VPVMLPRRSLLVMTGESRYLWTHGI 294

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BLAST of Lsi03G010570 vs. ExPASy Swiss-Prot
Match: Q07G10 (Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alkbh8 PE=2 SV=2)

HSP 1 Score: 63.9 bits (154), Expect = 3.5e-09
Identity = 56/195 (28.72%), Postives = 81/195 (41.54%), Query Frame = 0


Query: 95  GLWLCRIFLSPQHQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSN 154
           GL +   F+SP+ + ++L +I    W  E S  ++++   +  +  E             
Sbjct: 137 GLIIVEDFVSPEQERTMLESI---DWDSETSSQKSLKHRQVKHYGYEFR----------- 196

Query: 155 HMTDTLIVDRYNGDKVDCPLPS----------------DILWREPLFDQMIANVYQPGEG 214
                   D  N DK D PLP                  ++  +P  DQ+  N Y+PG+G
Sbjct: 197 -------YDNNNVDK-DKPLPGGLPDFCTEALRKCVQRGLIKHDP--DQLTINQYEPGQG 256

Query: 215 ICAHVDL-MRFEDGIAIVSLESPCVMHFTRVDETSCDPSIKGEVDLSASKVPVYLNPGSL 273
           I  HVD    FED I  +SL +  VM F                  + S VPV L   SL
Sbjct: 257 IPPHVDTHSAFEDEILSLSLGAEIVMDFKHP---------------NGSVVPVMLPQRSL 292

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BLAST of Lsi03G010570 vs. ExPASy Swiss-Prot
Match: Q96BT7 (Alkylated DNA repair protein alkB homolog 8 OS=Homo sapiens OX=9606 GN=ALKBH8 PE=1 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 1.3e-08
Identity = 37/88 (42.05%), Postives = 46/88 (52.27%), Query Frame = 0


Query: 186 DQMIANVYQPGEGICAHVDL-MRFEDGIAIVSLESPCVMHFTRVDETSCDPSIKGEVDLS 245
           DQM  N Y+PG+GI AH+D    FED I  +SL S  VM F   D  +            
Sbjct: 222 DQMTINQYEPGQGIPAHIDTHSAFEDEIVSLSLGSEIVMDFKHPDGIA------------ 281

Query: 246 ASKVPVYLNPGSLVILWGEARYLWKHEI 273
              VPV L   SL+++ GE+RYLW H I
Sbjct: 282 ---VPVMLPRRSLLVMTGESRYLWTHGI 294

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BLAST of Lsi03G010570 vs. ExPASy TrEMBL
Match: A0A0A0KV56 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001660 PE=4 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 1.1e-122
Identity = 213/231 (92.21%), Postives = 221/231 (95.67%), Query Frame = 0


Query: 44  MGEKLDDEDEILKQVFGDSSGDEGFEDQTVMNDLSYEVGHIHKWEQVKEIKGLWLCRIFL 103
           MGEKLDDE EILKQVFGDSS DE F D+TV ND SYE+GHIHKWEQVK+IKGLWLCR FL
Sbjct: 1   MGEKLDDEVEILKQVFGDSSEDEDFVDETVTNDSSYELGHIHKWEQVKQIKGLWLCRFFL 60

Query: 104 SPQHQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVD 163
           SPQ QSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVD
Sbjct: 61  SPQQQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVD 120

Query: 164 RYNGDKVDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM 223
           RYNGDK+DCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPC+M
Sbjct: 121 RYNGDKIDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCIM 180

Query: 224 HFTRVDETSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEINR 275
           HFT+VD+TSCDPSIKGEV+LS SKVPVYLNPGSLVILWGEARY WKHEINR
Sbjct: 181 HFTQVDKTSCDPSIKGEVNLSTSKVPVYLNPGSLVILWGEARYHWKHEINR 231

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BLAST of Lsi03G010570 vs. ExPASy TrEMBL
Match: A0A5A7TNC5 (Alkylated DNA repair protein alkB-like protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00950 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.4e-117
Identity = 207/231 (89.61%), Postives = 214/231 (92.64%), Query Frame = 0


Query: 44  MGEKLDDEDEILKQVFGDSSGDEGFEDQTVMNDLSYEVGHIHKWEQVKEIKGLWLCRIFL 103
           MGEKLDDE EILKQVFGDSS DE F D+TV  D S+E+GHIHKWEQVK+IKGLWLCR FL
Sbjct: 1   MGEKLDDEVEILKQVFGDSSEDEDFIDETVTTDSSFELGHIHKWEQVKQIKGLWLCRFFL 60

Query: 104 SPQHQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVD 163
           SPQ QSSLLSAIRNEGWFMEAS+NQAMRFGNLPTWAVELSDSVYEAVLSSNH TDT I D
Sbjct: 61  SPQQQSSLLSAIRNEGWFMEASENQAMRFGNLPTWAVELSDSVYEAVLSSNHKTDTSIAD 120

Query: 164 RYNGDKVDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM 223
            YNGDK+DCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM
Sbjct: 121 CYNGDKIDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM 180

Query: 224 HFTRVDETSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEINR 275
           HFT VDE+SCDPSIKGE  LSA KVPVYLNPGSLVILWGEARYLWKHEINR
Sbjct: 181 HFTHVDESSCDPSIKGEGILSALKVPVYLNPGSLVILWGEARYLWKHEINR 231

[Close]

BLAST of Lsi03G010570 vs. ExPASy TrEMBL
Match: A0A1S3BYI6 (uncharacterized protein LOC103494783 OS=Cucumis melo OX=3656 GN=LOC103494783 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.4e-117
Identity = 207/231 (89.61%), Postives = 214/231 (92.64%), Query Frame = 0


Query: 44  MGEKLDDEDEILKQVFGDSSGDEGFEDQTVMNDLSYEVGHIHKWEQVKEIKGLWLCRIFL 103
           MGEKLDDE EILKQVFGDSS DE F D+TV  D S+E+GHIHKWEQVK+IKGLWLCR FL
Sbjct: 1   MGEKLDDEVEILKQVFGDSSEDEDFIDETVTTDSSFELGHIHKWEQVKQIKGLWLCRFFL 60

Query: 104 SPQHQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVD 163
           SPQ QSSLLSAIRNEGWFMEAS+NQAMRFGNLPTWAVELSDSVYEAVLSSNH TDT I D
Sbjct: 61  SPQQQSSLLSAIRNEGWFMEASENQAMRFGNLPTWAVELSDSVYEAVLSSNHKTDTSIAD 120

Query: 164 RYNGDKVDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM 223
            YNGDK+DCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM
Sbjct: 121 CYNGDKIDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM 180

Query: 224 HFTRVDETSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEINR 275
           HFT VDE+SCDPSIKGE  LSA KVPVYLNPGSLVILWGEARYLWKHEINR
Sbjct: 181 HFTHVDESSCDPSIKGEGILSALKVPVYLNPGSLVILWGEARYLWKHEINR 231

[Close]

BLAST of Lsi03G010570 vs. ExPASy TrEMBL
Match: A0A6J1GAA7 (uncharacterized protein LOC111452220 OS=Cucurbita moschata OX=3662 GN=LOC111452220 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 2.0e-116
Identity = 202/239 (84.52%), Postives = 220/239 (92.05%), Query Frame = 0


Query: 36  QGPGEWREMGEKLDDEDEILKQVFGDSSGDEGFEDQTVMNDLSYEVGHIHKWEQVKEIKG 95
           +G  +WREMGE+LDDE EILKQVFGDSS DE  EDQTV+ D +YE GHI +WEQ KEIKG
Sbjct: 5   KGCDDWREMGEELDDEREILKQVFGDSSEDEDCEDQTVIGDSAYEGGHIRRWEQAKEIKG 64

Query: 96  LWLCRIFLSPQHQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNH 155
           LWLCR FLSPQ +SSLLSAIRNEGWFMEASQNQAMRFGNLPTWA ELSDS++EAVLSSNH
Sbjct: 65  LWLCRSFLSPQQESSLLSAIRNEGWFMEASQNQAMRFGNLPTWAAELSDSIHEAVLSSNH 124

Query: 156 MTDTLIVDRYNGDKVDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIV 215
           MTD LIVDR+NG K+DCPLPSDILWREPLFDQMIAN+YQPGEGICAHVDLMRFEDGIAIV
Sbjct: 125 MTDPLIVDRHNGVKIDCPLPSDILWREPLFDQMIANMYQPGEGICAHVDLMRFEDGIAIV 184

Query: 216 SLESPCVMHFTRVDETSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEINR 275
           SLESPCVMHFTRVDETS +P+ KGEVD+S SKVP+YLNPGS+V+LWGEARYLWKHEINR
Sbjct: 185 SLESPCVMHFTRVDETSRNPTSKGEVDVSMSKVPIYLNPGSVVLLWGEARYLWKHEINR 243

[Close]

BLAST of Lsi03G010570 vs. ExPASy TrEMBL
Match: A0A6J1KDK2 (uncharacterized protein LOC111492881 OS=Cucurbita maxima OX=3661 GN=LOC111492881 PE=4 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 9.9e-116
Identity = 200/231 (86.58%), Postives = 215/231 (93.07%), Query Frame = 0


Query: 44  MGEKLDDEDEILKQVFGDSSGDEGFEDQTVMNDLSYEVGHIHKWEQVKEIKGLWLCRIFL 103
           MGE+LDDE EILKQVFGDSS DE  EDQTV+ D +YE GHI +WEQ KEIKGLWLCR FL
Sbjct: 1   MGEELDDEREILKQVFGDSSEDEDCEDQTVIGDSAYEGGHIRRWEQAKEIKGLWLCRSFL 60

Query: 104 SPQHQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVD 163
           SPQ +SSLLSAIRNEGWFMEASQNQAMRFGNLPTWA ELSDS++EAVLSSNHMTD LIVD
Sbjct: 61  SPQQESSLLSAIRNEGWFMEASQNQAMRFGNLPTWAAELSDSIHEAVLSSNHMTDPLIVD 120

Query: 164 RYNGDKVDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM 223
           R+NG K+DCPLPSDILWREPLFDQMIAN+YQPGEGICAHVDLMRFEDGIAIVSLESPCVM
Sbjct: 121 RHNGVKIDCPLPSDILWREPLFDQMIANMYQPGEGICAHVDLMRFEDGIAIVSLESPCVM 180

Query: 224 HFTRVDETSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEINR 275
           HFTRVDETSCDP+ KGEVD+S SKVP+YLNPGS+V+LWGEARYLWKHEINR
Sbjct: 181 HFTRVDETSCDPTSKGEVDVSMSKVPIYLNPGSVVLLWGEARYLWKHEINR 231

[Close]

BLAST of Lsi03G010570 vs. NCBI nr
Match: XP_038905579.1 (uncharacterized protein LOC120091559 [Benincasa hispida])

HSP 1 Score: 460.3 bits (1183), Expect = 1.3e-125
Identity = 219/231 (94.81%), Postives = 224/231 (96.97%), Query Frame = 0


Query: 44  MGEKLDDEDEILKQVFGDSSGDEGFEDQTVMNDLSYEVGHIHKWEQVKEIKGLWLCRIFL 103
           MGEKLDDEDEILKQVFGDSS DE FEDQT+MND SYEVGHIH+WEQVKEIKGLWLC+IFL
Sbjct: 1   MGEKLDDEDEILKQVFGDSSDDEDFEDQTLMNDGSYEVGHIHQWEQVKEIKGLWLCKIFL 60

Query: 104 SPQHQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVD 163
           SPQ QSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSD VYEAVLSSNHMTDTL VD
Sbjct: 61  SPQQQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDFVYEAVLSSNHMTDTLTVD 120

Query: 164 RYNGDKVDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM 223
           RYNGDK+DCPLPS ILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM
Sbjct: 121 RYNGDKIDCPLPSHILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM 180

Query: 224 HFTRVDETSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEINR 275
           HFTRVDETSCDPSIKGEVDLSA+KVPVYLNPGSLVILWGEARYLWKHEINR
Sbjct: 181 HFTRVDETSCDPSIKGEVDLSATKVPVYLNPGSLVILWGEARYLWKHEINR 231

[Close]

BLAST of Lsi03G010570 vs. NCBI nr
Match: XP_004152257.1 (uncharacterized protein LOC101207942 [Cucumis sativus])

HSP 1 Score: 449.5 bits (1155), Expect = 2.3e-122
Identity = 213/231 (92.21%), Postives = 221/231 (95.67%), Query Frame = 0


Query: 44  MGEKLDDEDEILKQVFGDSSGDEGFEDQTVMNDLSYEVGHIHKWEQVKEIKGLWLCRIFL 103
           MGEKLDDE EILKQVFGDSS DE F D+TV ND SYE+GHIHKWEQVK+IKGLWLCR FL
Sbjct: 1   MGEKLDDEVEILKQVFGDSSEDEDFVDETVTNDSSYELGHIHKWEQVKQIKGLWLCRFFL 60

Query: 104 SPQHQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVD 163
           SPQ QSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVD
Sbjct: 61  SPQQQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVD 120

Query: 164 RYNGDKVDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM 223
           RYNGDK+DCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPC+M
Sbjct: 121 RYNGDKIDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCIM 180

Query: 224 HFTRVDETSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEINR 275
           HFT+VD+TSCDPSIKGEV+LS SKVPVYLNPGSLVILWGEARY WKHEINR
Sbjct: 181 HFTQVDKTSCDPSIKGEVNLSTSKVPVYLNPGSLVILWGEARYHWKHEINR 231

[Close]

BLAST of Lsi03G010570 vs. NCBI nr
Match: XP_008454361.1 (PREDICTED: uncharacterized protein LOC103494783 [Cucumis melo] >KAA0044368.1 alkylated DNA repair protein alkB-like protein 8 [Cucumis melo var. makuwa] >TYK29496.1 alkylated DNA repair protein alkB-like protein 8 [Cucumis melo var. makuwa])

HSP 1 Score: 432.6 bits (1111), Expect = 2.9e-117
Identity = 207/231 (89.61%), Postives = 214/231 (92.64%), Query Frame = 0


Query: 44  MGEKLDDEDEILKQVFGDSSGDEGFEDQTVMNDLSYEVGHIHKWEQVKEIKGLWLCRIFL 103
           MGEKLDDE EILKQVFGDSS DE F D+TV  D S+E+GHIHKWEQVK+IKGLWLCR FL
Sbjct: 1   MGEKLDDEVEILKQVFGDSSEDEDFIDETVTTDSSFELGHIHKWEQVKQIKGLWLCRFFL 60

Query: 104 SPQHQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVD 163
           SPQ QSSLLSAIRNEGWFMEAS+NQAMRFGNLPTWAVELSDSVYEAVLSSNH TDT I D
Sbjct: 61  SPQQQSSLLSAIRNEGWFMEASENQAMRFGNLPTWAVELSDSVYEAVLSSNHKTDTSIAD 120

Query: 164 RYNGDKVDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM 223
            YNGDK+DCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM
Sbjct: 121 CYNGDKIDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVM 180

Query: 224 HFTRVDETSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEINR 275
           HFT VDE+SCDPSIKGE  LSA KVPVYLNPGSLVILWGEARYLWKHEINR
Sbjct: 181 HFTHVDESSCDPSIKGEGILSALKVPVYLNPGSLVILWGEARYLWKHEINR 231

[Close]

BLAST of Lsi03G010570 vs. NCBI nr
Match: XP_022948590.1 (uncharacterized protein LOC111452220 [Cucurbita moschata])

HSP 1 Score: 428.7 bits (1101), Expect = 4.1e-116
Identity = 202/239 (84.52%), Postives = 220/239 (92.05%), Query Frame = 0


Query: 36  QGPGEWREMGEKLDDEDEILKQVFGDSSGDEGFEDQTVMNDLSYEVGHIHKWEQVKEIKG 95
           +G  +WREMGE+LDDE EILKQVFGDSS DE  EDQTV+ D +YE GHI +WEQ KEIKG
Sbjct: 5   KGCDDWREMGEELDDEREILKQVFGDSSEDEDCEDQTVIGDSAYEGGHIRRWEQAKEIKG 64

Query: 96  LWLCRIFLSPQHQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNH 155
           LWLCR FLSPQ +SSLLSAIRNEGWFMEASQNQAMRFGNLPTWA ELSDS++EAVLSSNH
Sbjct: 65  LWLCRSFLSPQQESSLLSAIRNEGWFMEASQNQAMRFGNLPTWAAELSDSIHEAVLSSNH 124

Query: 156 MTDTLIVDRYNGDKVDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIV 215
           MTD LIVDR+NG K+DCPLPSDILWREPLFDQMIAN+YQPGEGICAHVDLMRFEDGIAIV
Sbjct: 125 MTDPLIVDRHNGVKIDCPLPSDILWREPLFDQMIANMYQPGEGICAHVDLMRFEDGIAIV 184

Query: 216 SLESPCVMHFTRVDETSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEINR 275
           SLESPCVMHFTRVDETS +P+ KGEVD+S SKVP+YLNPGS+V+LWGEARYLWKHEINR
Sbjct: 185 SLESPCVMHFTRVDETSRNPTSKGEVDVSMSKVPIYLNPGSVVLLWGEARYLWKHEINR 243

[Close]

BLAST of Lsi03G010570 vs. NCBI nr
Match: XP_023524850.1 (uncharacterized protein LOC111788655 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 427.6 bits (1098), Expect = 9.2e-116
Identity = 201/239 (84.10%), Postives = 220/239 (92.05%), Query Frame = 0


Query: 36  QGPGEWREMGEKLDDEDEILKQVFGDSSGDEGFEDQTVMNDLSYEVGHIHKWEQVKEIKG 95
           +G  + REMGE+LDDE EILKQVFGDSS DE  EDQTV+ D +YE GHI +WEQ KEI+G
Sbjct: 5   KGCDDRREMGEELDDEREILKQVFGDSSEDEDCEDQTVIGDSAYEGGHIRRWEQAKEIRG 64

Query: 96  LWLCRIFLSPQHQSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNH 155
           LWLCR FLSPQ +SSLL+AIRNEGWFMEASQNQAMRFGNLPTWA ELSDS++EAVLSSNH
Sbjct: 65  LWLCRSFLSPQQESSLLAAIRNEGWFMEASQNQAMRFGNLPTWAAELSDSIHEAVLSSNH 124

Query: 156 MTDTLIVDRYNGDKVDCPLPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIV 215
           MTD LIVDR+NG K+DCPLPSDILWREPLFDQMIAN+YQPGEGICAHVDLMRFEDGIAIV
Sbjct: 125 MTDPLIVDRHNGVKIDCPLPSDILWREPLFDQMIANMYQPGEGICAHVDLMRFEDGIAIV 184

Query: 216 SLESPCVMHFTRVDETSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEINR 275
           SLESPCVMHFTRVDETSCDP+ KGEVD+S SKVP+YLNPGS+V+LWGEARYLWKHEINR
Sbjct: 185 SLESPCVMHFTRVDETSCDPTSKGEVDVSMSKVPIYLNPGSVVLLWGEARYLWKHEINR 243

[Close]

BLAST of Lsi03G010570 vs. TAIR 10
Match: AT4G02485.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 235.7 bits (600), Expect = 4.8e-62
Identity = 133/250 (53.20%), Postives = 161/250 (64.40%), Query Frame = 0


Query: 48  LDDEDEILKQVFGDSSGDEGFEDQTVMNDLSYEVGHIHKWEQVKEIKGLWLCRIFLSPQH 107
           +D+E E L+  FGDSS DE   D+     +   +G    WE+V+EI GLWL R FLS  H
Sbjct: 1   MDEEAENLRAAFGDSSDDEDIADRPGKETIG--IGDSAVWERVEEINGLWLYRNFLSIAH 60

Query: 108 QSSLLSAIRNEGWFMEASQNQAMRFGNLPTWAVELSDSVYEAVLSSNHMTDTLIVDRYNG 167
           QS LLSAI NEGWF+E S NQAMRFG+LP+WA ELSD + E +                 
Sbjct: 61  QSHLLSAILNEGWFVEESINQAMRFGDLPSWATELSDLIRETL----------------- 120

Query: 168 DKVDCP-LPSDILWREPLFDQMIANVYQPGEGICAHVDLMRFEDGIAIVSLESPCVMHFT 227
           + VD P L +D+LWREPLFDQ+I N+YQPGEGICAHVDL+RFEDGIAIVSLESPCVM F+
Sbjct: 121 ESVDLPVLSADLLWREPLFDQLIVNLYQPGEGICAHVDLLRFEDGIAIVSLESPCVMRFS 180

Query: 228 RVDETSCDPSIKGEVDLSASKVPVYLNPGSLVILWGEARYLWKHEINRYLQVIVLDLEKE 287
                   P+ K E +     V V LNPGSL+++ GEARY WKHEINR      L  E E
Sbjct: 181 --------PAEKNEYE----AVDVLLNPGSLILMSGEARYRWKHEINRKQNGFQL-WEGE 218

Query: 288 KSDKDADLSI 297
           + D+   +SI
Sbjct: 241 EIDQKRRISI 218

[Close]

BLAST of Lsi03G010570 vs. TAIR 10
Match: AT1G31600.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-10
Identity = 36/91 (39.56%), Postives = 48/91 (52.75%), Query Frame = 0


Query: 186 DQMIANVYQPGEGICAHVDL-MRFEDGIAIVSLESPCVMHFTRVDETSCDPS---IKGEV 245
           DQ+  N Y  G G+  H+D    FED I  +SL  PC+M F R   ++   S    +   
Sbjct: 295 DQLTVNEYPSGVGLSPHIDTHSAFEDCIFSLSLAGPCIMEFRRYSVSTWKASTTDAEKSG 354

Query: 246 DLSASKVPVYLNPGSLVILWGEARYLWKHEI 273
           D S  K  +YL P S+++L GEARY W H I
Sbjct: 355 DSSCIKKALYLPPRSMLLLSGEARYAWNHYI 385

[Close]

BLAST of Lsi03G010570 vs. TAIR 10
Match: AT1G31600.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-10
Identity = 36/91 (39.56%), Postives = 48/91 (52.75%), Query Frame = 0


Query: 186 DQMIANVYQPGEGICAHVDL-MRFEDGIAIVSLESPCVMHFTRVDETSCDPS---IKGEV 245
           DQ+  N Y  G G+  H+D    FED I  +SL  PC+M F R   ++   S    +   
Sbjct: 208 DQLTVNEYPSGVGLSPHIDTHSAFEDCIFSLSLAGPCIMEFRRYSVSTWKASTTDAEKSG 267

Query: 246 DLSASKVPVYLNPGSLVILWGEARYLWKHEI 273
           D S  K  +YL P S+++L GEARY W H I
Sbjct: 268 DSSCIKKALYLPPRSMLLLSGEARYAWNHYI 298

[Close]

BLAST of Lsi03G010570 vs. TAIR 10
Match: AT1G31600.3 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-10
Identity = 36/91 (39.56%), Postives = 48/91 (52.75%), Query Frame = 0


Query: 186 DQMIANVYQPGEGICAHVDL-MRFEDGIAIVSLESPCVMHFTRVDETSCDPS---IKGEV 245
           DQ+  N Y  G G+  H+D    FED I  +SL  PC+M F R   ++   S    +   
Sbjct: 295 DQLTVNEYPSGVGLSPHIDTHSAFEDCIFSLSLAGPCIMEFRRYSVSTWKASTTDAEKSG 354

Query: 246 DLSASKVPVYLNPGSLVILWGEARYLWKHEI 273
           D S  K  +YL P S+++L GEARY W H I
Sbjct: 355 DSSCIKKALYLPPRSMLLLSGEARYAWNHYI 385

The following BLAST results are available for this feature:

BLAST of Lsi03G010570 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RWY1	1.6e-09	39.56	Alkylated DNA repair protein ALKBH8 homolog OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q9UT12	2.0e-09	39.22	Uncharacterized protein P8A3.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]
Q95K79	2.7e-09	43.18	Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=AL...	[more]
Q07G10	3.5e-09	28.72	Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alk...	[more]
Q96BT7	1.3e-08	42.05	Alkylated DNA repair protein alkB homolog 8 OS=Homo sapiens OX=9606 GN=ALKBH8 PE...	[more]

BLAST of Lsi03G010570 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KV56	1.1e-122	92.21	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]
A0A5A7TNC5	1.4e-117	89.61	Alkylated DNA repair protein alkB-like protein 8 OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BYI6	1.4e-117	89.61	uncharacterized protein LOC103494783 OS=Cucumis melo OX=3656 GN=LOC103494783 PE=...	[more]
A0A6J1GAA7	2.0e-116	84.52	uncharacterized protein LOC111452220 OS=Cucurbita moschata OX=3662 GN=LOC1114522...	[more]
A0A6J1KDK2	9.9e-116	86.58	uncharacterized protein LOC111492881 OS=Cucurbita maxima OX=3661 GN=LOC111492881...	[more]

BLAST of Lsi03G010570 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905579.1	1.3e-125	94.81	uncharacterized protein LOC120091559 [Benincasa hispida]	[more]
XP_004152257.1	2.3e-122	92.21	uncharacterized protein LOC101207942 [Cucumis sativus]	[more]
XP_008454361.1	2.9e-117	89.61	PREDICTED: uncharacterized protein LOC103494783 [Cucumis melo] >KAA0044368.1 alk...	[more]
XP_022948590.1	4.1e-116	84.52	uncharacterized protein LOC111452220 [Cucurbita moschata]	[more]
XP_023524850.1	9.2e-116	84.10	uncharacterized protein LOC111788655 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi03G010570 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02485.1	4.8e-62	53.20	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G31600.1	1.1e-10	39.56	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT1G31600.2	1.1e-10	39.56	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT1G31600.3	1.1e-10	39.56	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 81..280 e-value: 7.6E-29 score: 102.8
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 185..277 e-value: 1.3E-7 score: 32.1
None	No IPR available	PANTHER	PTHR13069	UNCHARACTERIZED	coord: 49..277
None	No IPR available	PANTHER	PTHR13069:SF32	ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8 ISOFORM X1	coord: 49..277
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 80..275
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 184..301 score: 8.317449

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G010570.1	Lsi03G010570.1	mRNA