Lsi03G010480 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G010480
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionHXXXD-type acyl-transferase family protein
Locationchr03: 20136029 .. 20139916 (-)
RNA-Seq ExpressionLsi03G010480
SyntenyLsi03G010480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGATGCAACTTGCAATAGTTAGCAAAGAAACCATAAAGCCTTTGTTTTCAACCCCACCAGAGCTCAGGCTTCACCATCTTTGTTTGTTTGATCGTCATGCCCCTTACCTTTATCTCCAATTTCTTTATTTCTATGAAAATGGCTCCAACTCCAAGTTGTTGAAGCATAGCCTTTCTAAAGTCTTGACATTCTACTACCCTTTTGCGGGCCGCCTCAAAGACGACTGCTTCGTCGAATGCAACGACACGGGTGTCACCTTCTTAGAGGCTGAGCTCCGGTGTTCGATGTCGGAAATTATGAACGTCTACAATTTGCGGCACGATGAAGTTTTGAAGTTGATTTGTTTTGATGATATGAATGGAAAGGACCAAAGATACAATCCTTTGTTGTGTGTTCAGTTGACTAGATTTGAATGTGGAGGAGAAGCAATTTGTGTGTTCTTGTCCCACAAACTTGCTGATGCTTCCACATTTACTAACTTCATGAGCCATTGGGCATCCGTTTCCCGCAGCTCCGACATGCCACCGTTGGCGCCTCCTCGGTTTGACGCTGCGAGTTTATTCGAAGCAGCCGTCGCAGACGATGTACTAGGTGTTCATAATCTCTACTAAATTCATGTGAATTTATTACTCTCTCTAGTTTTATGAGTTTTTTTTTTTTTTTTTTTTTTTTTGAGTATCTTATTCACACACACATATATATTAGATTAATTTGAATTTTGTGAATATCTAAGGATATTTGAATTGTTTTTAGATTTTAGGATTCACGAAGCGAAAATCTTGAGAAAAAAAATAGTGTGTTTGGATGATGCTTTTTGAAAGAAAAAAAAAACGTTTTTAAGAATAAATATACTTTCGAAATAATAGATTTTTTTAAAAAAAAAGAAAAAAAATTAAATATAGGATATGTTTGGAAGTAATTTTAAAATTCCTTATTTATTAGGTTGAAAATCAGCTTTAGTTTTTTAAAATTAATTTAATATTTAATCTATTTTTAAAATTTTTCATCCCATGTTTAATTATAAGAAAAAAAATTAAAAGCATTTTTAAGCATTTTTAAGAGGTACTACCTATTGAAAATGTTTAAACATTTTAAAACTAAGCACTACCAATTTTTTTTAAAAAAAAAAAATCATGCATGAGCCTTTTAAAAGACATGTCTTACAAACACAGGTGTTTTTATATTTAAAGTATGCGGCTAAATACAAAAATAGTGAAATTAAAGCTGTCACTATTGGAAAAAGTTTATTTATAACCTTAAAACTGAGTCAATTGTTTTAACCTAAACTTTTAATTTCATCGATTTAAATTTTAAATTTGTCATATAGTATCCATTTTAATTCTAAACTTTCTATTTCGTCAATTCAAATTTTAAGTTGAAGTACTTATATCAATTTAGACCTTCGATTAAGTCCCATTAGAGTTAAAATTATGAATACAATTTATCAAGTATAAAGTTTAAATTGATGAAATTGAAACAATATGTAAAATTTAATATTTAAATTGATAAAATTGAAAGTTAGAATTTAATTGATACAACTAGCTAAAGTTTAGAATTAAAAAAATATATGATAATTTCCTTTTTTTTTCATCAACCCATTTCATTTTCAAAATATTTCTTTTATATCACCATCTCAATATATATATAGTACACATTGTTAATCATTCATTCATATTGTTAATATACTTGAAGGAGAAGGTTCAAAAATAGTAAAACGAGACAAAAATTTTGTGTGTGTTCGAAGGTTCAAAGATTAAAGCTCTTAAAGCAATGGTTTCTGATAAAGTGGAGAATCCAAGTCGTGTACAAATTCTTGCTGCTTTCATCTACAAAGCTGCAATTTCTGCATCAATGGTAGCAAGTGATTCGAAAGATCACCCCCACCAACTATTTGCTAAGAATCGCTATAAACTTACGCACTAGAGTGATTCCGCCGATGCCCGCAACTTTATCAGGTCTGTTATAAACTGCTATTAAACTCCAAAACTTAAAAAGATATTGTTTGATAATTATTTAGTTTTTGATATTTACATAAACTTGGATGGTTTTCAAAGATCGTTTACTATGTTTTTCATTTTTTTCAAGTAAACATTTGAATTATCCTTGGTCAAATTTTAAAAGCAAAACAAATTTTCAAAAACTATTTTTTTTTTAGTTTTTAAAACTTGACTTCTTGACTTTTGAAAACATACGTAGAAAAATTAAAAGAAAACAATATAAATGGAAATGCAAGAATTAACATAGAAAAACTCTAAATCGAAGAAAAAACCTCAGATTAGAAAATTTTCATTATGTGAAAAATTATTATTATTATCACACAAAGACCTCTCCGAAACCCCAATTACAAAAAATAAATAAAAAAAAACCTTTTCAAAATATTATATCACTCACACAACTGAACTAGTATACCATGTTTAAAGTGCTTCAAATTGGAATGATTAGAAATCCGCCCCTTGTAATGCTTGAAACTCATCACTTCCTTTCAACCTTTCATATGTGAGACACCTACTCTTTTAATATTTTGCCAAAATCCAACATTAGACTAATAGGTTAATGGTTTATTTAATATTGTATCCAATATTCTTAACCAGCTCTCTCGATCAACTCAATTTTTTTTTGATATCCGTGAGTGTTCGAGCCAACTTACGCGTTCTTCGACCAATTTCATAGAACAACCAGTCTGATCCTATAACATTTGGGTGTTAAGGAAACCTATAGGATATTAAATCCTATAAGTAGGTGACCACCATCGATTGAAGCTATCACCCCTTAGTCCTTTAGCCTGCAAAACTCTGATTTGGTATTATGTTAAATGAACCCTTAACTCAAAATCAAAAGGCTTAAGTTGATATATGTCAATATTTTATCCATATTCTCAACTCTTTTCTTGACTGTATAACATTTTGAATCTCAAAACTTACTAGAAAAATGTAACATTTTAAAATCTAAAGATCAAATAGAAAATAGACTAAAAAATTAAAGAATAAAAGTGTAATATTTGAAGGGATCAAAAAGGTTTTTCTTTCTCTATTTTATTTTATATTTTTGACGGAATAGACAAATATTTTTAAATTTGTGAAATAGTTTCGCAAATTTTTCACCCGTATTTTTAATCATTTATTTGTATTTTTATTGAAGGCAACATAATCTCTTTCTTCCTGACGTCGTCAACGACGGCAGAGCATAAGGAGATAGATCTATCGAGTTTGTTGAATGACATGAAGAGAAACATGGAAGAGTTCTGCAACAAATATCCAAAACATTACAAAGCGGAAGAATGGTCTTGTCTTTACAAGTTGCATATGAAAGAATCAATTGAGAAGATGAGCAATCCAAAGGATCAAACTGTATATGCATGCAGCAGTTGGTGCAGATTTCCATTTTACGATGCTGATTTTGGGTGGGGGAAGCCTCTATGGATCACTGCGCCTGTTATTTTGAGTAAGAATGTCATTATATTGATGGATGCCAAAGATGGTGAGGGAATTGAAGCATTGGTGACTTTGGAGAAGGAGGAAATGGAAGTATTTGGGCAAAATGAGGAGCTTCTTTCCTATTGTGAACTCAAAAGTTGTTAGGGGTTTGGTATTGGTCATATTTTTTAATTGTGATTCGTGGTTGTTGGTAACCTTGTTTTTTATCTTGTTTCCCGTGATGTGTGTCATCTTGTCTCATTGTGATGTTCTACGGGATGCCTCACACAATGTTGTTTTATTTGGAAATTCTTTCCTTTTTTGAAAAATTATGTGTCGTTATTCTTAAGGATTTTTTCTTTCTTTGAGGCTTGACCGATTACGGTAATGGTGTATTTCTTCCCTTTTTTGTGGATATATATTTAAGTGTTTGTATTGTGCGTTTTAGGGTTACTACTTTGAGGAAAAAGTCT

mRNA sequence

AATGATGCAACTTGCAATAGTTAGCAAAGAAACCATAAAGCCTTTGTTTTCAACCCCACCAGAGCTCAGGCTTCACCATCTTTGTTTGTTTGATCGTCATGCCCCTTACCTTTATCTCCAATTTCTTTATTTCTATGAAAATGGCTCCAACTCCAAGTTGTTGAAGCATAGCCTTTCTAAAGTCTTGACATTCTACTACCCTTTTGCGGGCCGCCTCAAAGACGACTGCTTCGTCGAATGCAACGACACGGGTGTCACCTTCTTAGAGGCTGAGCTCCGGTGTTCGATGTCGGAAATTATGAACGTCTACAATTTGCGGCACGATGAAGTTTTGAAGTTGATTTGTTTTGATGATATGAATGGAAAGGACCAAAGATACAATCCTTTGTTGTGTGTTCAGTTGACTAGATTTGAATGTGGAGGAGAAGCAATTTGTGTGTTCTTGTCCCACAAACTTGCTGATGCTTCCACATTTACTAACTTCATGAGCCATTGGGCATCCGTTTCCCGCAGCTCCGACATGCCACCGTTGGCGCCTCCTCGGTTTGACGCTGCGAGTTTATTCGAAGCAGCCGTCGCAGACGATGATTCACGAAGCGAAAATCTTGAGAAAAAAAATAGTTCAAAGATTAAAGCTCTTAAAGCAATGGTTTCTGATAAAGTGGAGAATCCAAGTCGTGTACAAATTCTTGCTGCTTTCATCTACAAAGCTGCAATTTCTGCATCAATGGTAGCAAGCAACATAATCTCTTTCTTCCTGACGTCGTCAACGACGGCAGAGCATAAGGAGATAGATCTATCGAGTTTGTTGAATGACATGAAGAGAAACATGGAAGAGTTCTGCAACAAATATCCAAAACATTACAAAGCGGAAGAATGGTCTTGTCTTTACAAGTTGCATATGAAAGAATCAATTGAGAAGATGAGCAATCCAAAGGATCAAACTGTATATGCATGCAGCAGTTGGTGCAGATTTCCATTTTACGATGCTGATTTTGGGTGGGGGAAGCCTCTATGGATCACTGCGCCTGTTATTTTGAGTAAGAATGTCATTATATTGATGGATGCCAAAGATGGTGAGGGAATTGAAGCATTGGTGACTTTGGAGAAGGAGGAAATGGAAGTATTTGGGCAAAATGAGGAGCTTCTTTCCTATTGTGAACTCAAAAGTTGTTAGGGGTTTGGTATTGGTCATATTTTTTAATTGTGATTCGTGGTTGTTGGTAACCTTGTTTTTTATCTTGTTTCCCGTGATGTGTGTCATCTTGTCTCATTGTGATGTTCTACGGGATGCCTCACACAATGTTGTTTTATTTGGAAATTCTTTCCTTTTTTGAAAAATTATGTGTCGTTATTCTTAAGGATTTTTTCTTTCTTTGAGGCTTGACCGATTACGGTAATGGTGTATTTCTTCCCTTTTTTGTGGATATATATTTAAGTGTTTGTATTGTGCGTTTTAGGGTTACTACTTTGAGGAAAAAGTCT

Coding sequence (CDS)

ATGATGCAACTTGCAATAGTTAGCAAAGAAACCATAAAGCCTTTGTTTTCAACCCCACCAGAGCTCAGGCTTCACCATCTTTGTTTGTTTGATCGTCATGCCCCTTACCTTTATCTCCAATTTCTTTATTTCTATGAAAATGGCTCCAACTCCAAGTTGTTGAAGCATAGCCTTTCTAAAGTCTTGACATTCTACTACCCTTTTGCGGGCCGCCTCAAAGACGACTGCTTCGTCGAATGCAACGACACGGGTGTCACCTTCTTAGAGGCTGAGCTCCGGTGTTCGATGTCGGAAATTATGAACGTCTACAATTTGCGGCACGATGAAGTTTTGAAGTTGATTTGTTTTGATGATATGAATGGAAAGGACCAAAGATACAATCCTTTGTTGTGTGTTCAGTTGACTAGATTTGAATGTGGAGGAGAAGCAATTTGTGTGTTCTTGTCCCACAAACTTGCTGATGCTTCCACATTTACTAACTTCATGAGCCATTGGGCATCCGTTTCCCGCAGCTCCGACATGCCACCGTTGGCGCCTCCTCGGTTTGACGCTGCGAGTTTATTCGAAGCAGCCGTCGCAGACGATGATTCACGAAGCGAAAATCTTGAGAAAAAAAATAGTTCAAAGATTAAAGCTCTTAAAGCAATGGTTTCTGATAAAGTGGAGAATCCAAGTCGTGTACAAATTCTTGCTGCTTTCATCTACAAAGCTGCAATTTCTGCATCAATGGTAGCAAGCAACATAATCTCTTTCTTCCTGACGTCGTCAACGACGGCAGAGCATAAGGAGATAGATCTATCGAGTTTGTTGAATGACATGAAGAGAAACATGGAAGAGTTCTGCAACAAATATCCAAAACATTACAAAGCGGAAGAATGGTCTTGTCTTTACAAGTTGCATATGAAAGAATCAATTGAGAAGATGAGCAATCCAAAGGATCAAACTGTATATGCATGCAGCAGTTGGTGCAGATTTCCATTTTACGATGCTGATTTTGGGTGGGGGAAGCCTCTATGGATCACTGCGCCTGTTATTTTGAGTAAGAATGTCATTATATTGATGGATGCCAAAGATGGTGAGGGAATTGAAGCATTGGTGACTTTGGAGAAGGAGGAAATGGAAGTATTTGGGCAAAATGAGGAGCTTCTTTCCTATTGTGAACTCAAAAGTTGTTAG

Protein sequence

MMQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYENGSNSKLLKHSLSKVLTFYYPFAGRLKDDCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKLICFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSSDMPPLAPPRFDAASLFEAAVADDDSRSENLEKKNSSKIKALKAMVSDKVENPSRVQILAAFIYKAAISASMVASNIISFFLTSSTTAEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKESIEKMSNPKDQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVTLEKEEMEVFGQNEELLSYCELKSC
Homology
BLAST of Lsi03G010480 vs. ExPASy Swiss-Prot
Match: A0A2G3D6U1 (Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 5.0e-48
Identity = 138/430 (32.09%), Postives = 216/430 (50.23%), Query Frame = 0

Query: 4   LAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFY-------ENGSNSKLLKH 63
           +++  K  IKP   TP  LR H L   D+    +Y+   +FY       E+  NS  L H
Sbjct: 10  VSVCDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 64  -------SLSKVLTFYYPFAGRLKDDCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDE 123
                  SLS+ L  YYP+AG+LKD+  V+CND G  FL   ++CSMSEI++  +    E
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAE 129

Query: 124 VLKLICFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVS 183
            + ++  D     +     LL VQ+++F+CGG AI V  SHK+ D  +  NF++ W+SV+
Sbjct: 130 SI-VLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVT 189

Query: 184 RSSDMPPLAP-PRFDAASLFEAAVADDDSRSENLEKKN----------SSKIKALKAMVS 243
           R      L P PRF   S+F        +  + L   N          + K+ AL+A V+
Sbjct: 190 RDHTTTTLVPSPRFVGDSVFSTKTYGSLTTPQILSDLNECVQKRLIFPTDKLDALRAKVA 249

Query: 244 DK--VENPSRVQILAAFIYKAA--ISASMVASNIISFF----LTSSTTAEHKEIDLSSLL 303
           ++  V+NP+R ++++A ++K A   S+SM+ S ++ F     +       +   +LSS+ 
Sbjct: 250 EESGVKNPTRAEVVSALLFKCATKASSSMLPSKLVHFLNIRTMIKPRLPRNTIGNLSSIF 309

Query: 304 NDMKRNMEEFCNKYPKHYKAEEWSCLYK----------LHMKESIEKMSNPKD-----QT 363
           +    NM++           +E    YK          L + ES+ +   P +     + 
Sbjct: 310 SIEATNMQDMELPTLVRNLRKEVEVAYKKDQVEQNELILEVVESMREGKLPFENMDGYEN 369

Query: 364 VYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVTLEKEEMEV 386
           VY CS+ C++P+Y  DFGWG+P  +      SKN   L D K G+G+EA V L K++M  
Sbjct: 370 VYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQGVEARVMLHKQQMSE 429

BLAST of Lsi03G010480 vs. ExPASy Swiss-Prot
Match: Q58VT1 (Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.4e-47
Identity = 139/430 (32.33%), Postives = 218/430 (50.70%), Query Frame = 0

Query: 4   LAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFY-------ENGSNSKLLKH 63
           ++I  K  IKP   TP  LR H L   D+    +Y+   +FY       E+  NS  L H
Sbjct: 10  VSICDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 64  -------SLSKVLTFYYPFAGRLKDDCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDE 123
                  SLS+ L  YYP+AG+LKD+  V+CND G  FL   ++CSMSEI++  +    E
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAE 129

Query: 124 VLKLICFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVS 183
            + ++  D     +     LL VQ+++F+CGG AI V  SHK+ D  +  NF++ W+SV+
Sbjct: 130 SI-VLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVT 189

Query: 184 RSSDMPPLAP-PRFDAASLFEAAVADD-------DSRSENLEKK---NSSKIKALKAMVS 243
           R      L P PRF   S+F                 +E ++K+    + K+ AL+A V+
Sbjct: 190 RDHTTTTLVPSPRFVGDSVFSTKKYGSLITPQILSDLNECVQKRLIFPTDKLDALRAKVA 249

Query: 244 DK--VENPSRVQILAAFIYKAA--ISASMVASNIISFF----LTSSTTAEHKEIDLSSLL 303
           ++  V+NP+R ++++A ++K A   S+SM+ S ++ F     +       +   +LSS+ 
Sbjct: 250 EESGVKNPTRAEVVSALLFKCATKASSSMLPSKLVHFLNIRTMIKPRLPRNAIGNLSSIF 309

Query: 304 NDMKRNMEEFCNKYPKHYKAEEWSCLYK----------LHMKESIEKMSNPKD-----QT 363
           +    NM++           +E    YK          L + ES+ +   P +     + 
Sbjct: 310 SIEATNMQDMELPTLVRNLRKEVEVAYKKDQVEQNELILEVVESMREGKLPFENMDGYEN 369

Query: 364 VYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVTLEKEEMEV 386
           VY CS+ C++P+Y  DFGWG+P  +      SKN   L D K G+G+EA V L K++M  
Sbjct: 370 VYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQGVEARVMLHKQQMSE 429

BLAST of Lsi03G010480 vs. ExPASy Swiss-Prot
Match: D2Y3X2 (Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.9e-47
Identity = 138/430 (32.09%), Postives = 216/430 (50.23%), Query Frame = 0

Query: 4   LAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFY-------ENGSNSKLLKH 63
           +++ +K  IKP   TP  LR H L   D+    +Y+   +FY       E+  NS  L H
Sbjct: 10  VSVCNKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 64  -------SLSKVLTFYYPFAGRLKDDCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDE 123
                  SLS+ L  YYP+AG+LKD+  V+CND G  FL   ++CSMSEI++  +    E
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAE 129

Query: 124 VLKLICFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVS 183
            + ++  D     +     LL VQ+++F+CGG AI V  SHK+ D  +  NF++ W+SV+
Sbjct: 130 SI-VLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVT 189

Query: 184 RSSDMPPLAP-PRFDAASLFEAAVADDDSRSENLEKKN----------SSKIKALKAMVS 243
           R      L P PRF   S+F           + L   N          + K+ AL+A V+
Sbjct: 190 RDRTTTTLVPSPRFVGDSVFSTQKYGSLITPQILSDLNQCVQKRLIFPTDKLDALRAKVA 249

Query: 244 DK--VENPSRVQILAAFIYKAA--ISASMVASNIISFF----LTSSTTAEHKEIDLSSLL 303
           ++  V+NP+R ++++A ++K A   S+SM+ S ++ F     +       +   +LSS+ 
Sbjct: 250 EESGVKNPTRAEVVSALLFKCATKASSSMLPSKLVHFLNIRTMIKPRLPRNAIGNLSSIF 309

Query: 304 NDMKRNMEEFCNKYPKHYKAEEWSCLYK----------LHMKESIEKMSNPKD-----QT 363
           +    NM++           +E    YK          L + ES+ +   P +     + 
Sbjct: 310 SIEATNMQDMELPTLVRNLRKEVEVAYKKDQVEQNELILEVVESMREGKLPFENMDGYKN 369

Query: 364 VYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVTLEKEEMEV 386
           VY CS+ C++P+Y  DFGWG+P  +      SKN   L D K G+G+EA V L K++M  
Sbjct: 370 VYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQGVEARVMLHKQQMSE 429

BLAST of Lsi03G010480 vs. ExPASy Swiss-Prot
Match: K4D9Y4 (Acylsugar acyltransferase 3 OS=Solanum lycopersicum OX=4081 GN=ASAT3 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.1e-42
Identity = 126/429 (29.37%), Postives = 218/429 (50.82%), Query Frame = 0

Query: 1   MMQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFY----ENGSN--SKLL 60
           M    I+S++ IK L  TP  LR H L   D     L+  + +FY    +N SN  S++L
Sbjct: 1   MASSTIISRKMIKLLSPTPSSLRCHKLSFMDHINFPLHSPYAFFYPKIPQNYSNKISQVL 60

Query: 61  KHSLSKVLTFYYPFAGRLKDD-CFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKL 120
           ++SLSKVL+FYYP AG++ ++  +V+CNDTG  +L   + C MS+I+   N  +++V+ +
Sbjct: 61  ENSLSKVLSFYYPLAGKINNNYTYVDCNDTGAEYLNVRIDCPMSQIL---NHPYNDVVDV 120

Query: 121 ICFDDM--NGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRS 180
           +   D+  +      +PL+ VQL+ F+CGG A+    SH + D    + F++ WAS +R+
Sbjct: 121 VFPQDLPWSSSSLTRSPLV-VQLSHFDCGGVAVSACTSHTIFDGYCLSKFINDWASTARN 180

Query: 181 SDMPPLAPPRFDAASLFE-----------AAVADDDSRSENLEKKNSSKIKALKAMVSDK 240
            +  P   P+F+A++ F             A          +   +SS +  LK +V+ +
Sbjct: 181 MEFKP--SPQFNASTFFPLPSETNLSSTLPATRPSQRHVSRMYNFSSSNLTRLKDIVTKE 240

Query: 241 --VENPSRVQILAAFIYKAAISASMVASNIISFFLTS----------------------S 300
             V+NP+RV++ +A ++K  ++ SM +S +    L S                      +
Sbjct: 241 SHVKNPTRVEVASALVHKCGVTMSMESSGMFKPTLMSHAMNLRPPIPLNTMGNATCIILT 300

Query: 301 TTAEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKESIEKMSNPKDQTV 360
           T     E+ L + +  ++++ ++  +K  K  K E+   LY L + ++   +       V
Sbjct: 301 TAMTEDEVKLPNFVAKLQKDKQQLRDKL-KDMK-EDRMPLYTLELGKNAMNIIEKDTHDV 360

Query: 361 YACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVTLEKEEMEVF 386
           Y CS       +  DFGWG+P+ +T     +KN  I MD + G+G+  L+TL K++M  F
Sbjct: 361 YLCSGMTNTGLHKIDFGWGEPVRVTLATHPNKNNFIFMDEQSGDGLNVLITLTKDDMLKF 420

BLAST of Lsi03G010480 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 5.3e-42
Identity = 125/423 (29.55%), Postives = 213/423 (50.35%), Query Frame = 0

Query: 3   QLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYENGSNSKL--------L 62
           Q+ I+S+E I+P   TP  L+ H L   D+     ++  + FY N  +S L        L
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRSENL 63

Query: 63  KHSLSKVLTFYYPFAGRLKDDCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKLI 122
           K SLS VLT +YP AGR+  +  V+CND+GV FLEA +   +SE   + N+  DE+ + +
Sbjct: 64  KRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEA--IKNVAIDELNQYL 123

Query: 123 CFDDMNGKDQ---RYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRS 182
            F    G ++   + +  L V+++ FECGG AI V +SHK+ADA +   F++ W +  + 
Sbjct: 124 PFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATCQ- 183

Query: 183 SDMPPLAPPRFDAAS----LFEAAVADDDSRSENLEKK----NSSKIKALKAMVSD---K 242
            +   +  P FD  S      E+  A +    EN+  K    +  K++ALKA ++    +
Sbjct: 184 -EETDIVQPNFDLGSHHFPPMESIPAPEFLPDENIVMKRFVFDKEKLEALKAQLASSATE 243

Query: 243 VENPSRVQILAAFIYKAAISAS---------MVASNIISF---------------FLTSS 302
           V+N SRVQI+ A I+K  I  +         +VA+  ++                  T +
Sbjct: 244 VKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAAQAVNLRSRMNPPFPQSAMGNIATMA 303

Query: 303 TTAEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKESIEKMSNPKDQTV 362
                ++ D S L+  +K ++ +  +++ K  +       Y+             + Q +
Sbjct: 304 YAVAEEDKDFSDLVGPLKTSLAKIDDEHVKELQKGVTYLDYE------------AEPQEL 363

Query: 363 YACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVTLEKEEMEVF 380
           ++ SSWCR  FYD DFGWGKP+ +    +  KN++ LMD ++ +G+EA +++ ++EM + 
Sbjct: 364 FSFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWISMAEDEMSML 410

BLAST of Lsi03G010480 vs. ExPASy TrEMBL
Match: A0A0A0KV47 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G001560 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 6.1e-174
Identity = 322/441 (73.02%), Postives = 351/441 (79.59%), Query Frame = 0

Query: 1   MMQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYENGSNSKLLKHSLSK 60
           MMQL IVSKETIKPLFSTPPEL+LHHLCLFDRHAPYLYL FLYFY+N S+SKLLKHSLSK
Sbjct: 1   MMQLEIVSKETIKPLFSTPPELKLHHLCLFDRHAPYLYLHFLYFYQNSSHSKLLKHSLSK 60

Query: 61  VLTFYYPFAGRLKDDCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKLICFDDMN 120
            LTFYYPFAGRLKDDC ++CND G T LEA LRC MSE MNVYNLRHDE LKL+CFDDMN
Sbjct: 61  ALTFYYPFAGRLKDDCSIDCNDMGATILEARLRCPMSEFMNVYNLRHDEALKLVCFDDMN 120

Query: 121 --GKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSS--DMPP 180
              KDQRYNPLLCVQLTRFECGGE +CVFLSHKLADASTFTNFM+HWAS+SR+   DMP 
Sbjct: 121 DGSKDQRYNPLLCVQLTRFECGGEVLCVFLSHKLADASTFTNFMNHWASLSRTGDHDMPL 180

Query: 181 LAPPRFDAASLFEAAVADD--------DSRSENLEKK----NSSKIKALKAMVSDKVENP 240
           L PPRFDA SLFEAA  DD          R +NL  K     +SKI ALKA V DKVENP
Sbjct: 181 LPPPRFDAGSLFEAATVDDVLGEGSKIVKREKNLVSKRMVFKASKIAALKATVCDKVENP 240

Query: 241 SRVQILAAFIYKAAISAS----------------------------------MVASNIIS 300
           SRVQILAAFIYKAAISAS                                   ++ NIIS
Sbjct: 241 SRVQILAAFIYKAAISASKSTLSIHHSRTPITNYLLRIAINLRTRLIPPMPATLSGNIIS 300

Query: 301 FFLTS-STTAEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKESIEKMS 360
           FFLTS +TTAE KEI+LSSLL+DMKRNM+EFCNKYP++YKAEEWS LYKLH+KES+E+M+
Sbjct: 301 FFLTSATTTAEQKEIELSSLLSDMKRNMKEFCNKYPRNYKAEEWSSLYKLHIKESMERMN 360

Query: 361 NPKDQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVTLE 391
           NP+DQ VYACSSWCRFPFYDADFGWGKP+WIT P ILSKNVIILMDAKDGEGIEA V+LE
Sbjct: 361 NPEDQIVYACSSWCRFPFYDADFGWGKPVWITTPTILSKNVIILMDAKDGEGIEAFVSLE 420

BLAST of Lsi03G010480 vs. ExPASy TrEMBL
Match: A0A6J1F5C1 (vinorine synthase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442451 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 1.3e-115
Identity = 236/446 (52.91%), Postives = 289/446 (64.80%), Query Frame = 0

Query: 2   MQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYEN--------GSNSKL 61
           M+L IVSKETIKPL STPP+L    L  FD+H P +Y   LYFY N        G   +L
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCRGRL 60

Query: 62  LKHSLSKVLTFYYPFAGRLKD-DCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLK 121
           LK SLSK LTFYYPFAGRL+D   F++CND G TF+EA+LRC MSE+MN ++L HDE+LK
Sbjct: 61  LKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFVEAKLRCPMSEVMNTFDLNHDEILK 120

Query: 122 LICFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSS 181
           L+CF+D+ GK Q ++PLL VQLT+FECGGE + V L+HK+AD +TF NFM+ WAS++RSS
Sbjct: 121 LLCFEDIKGKVQTFDPLLSVQLTQFECGGEVMYVSLAHKVADLATFANFMNDWASIARSS 180

Query: 182 D--MPPLAPPRFDAASLF-----------EAAVADDDSRSENLEKK----NSSKIKALKA 241
               PP+  P F AASLF           E   +    R EN+  +      SKI+ALKA
Sbjct: 181 GGIDPPVVCPLFHAASLFRPELDGGVDPSEGEGSGSKKREENVRSRRMVFEGSKIRALKA 240

Query: 242 MVSDKVENPSRVQILAAFIYKAAISASMVAS----------------------------- 301
           MVS KVENP+RVQ+L AFIYKA +SA    S                             
Sbjct: 241 MVSKKVENPTRVQVLTAFIYKAIVSAKNALSGGLSREPTSILQFINLRSRVEPPLPGTLT 300

Query: 302 -NIISFFLTSSTTAE-HKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKES 361
            NI+SFF  SST+AE  +E++L S++ DMKRN EEFC K P++Y+ EE S   K  +KES
Sbjct: 301 GNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEEFCRKLPRNYRDEEGSPALKSWIKES 360

Query: 362 IEKMSNPKDQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEA 391
            E      D   Y CSSWCRFP Y+ADFGWG P+WI  P  LSKN I LMDAKDGEGIEA
Sbjct: 361 FETWMMDNDGRKYGCSSWCRFPLYEADFGWGTPIWIAVPAFLSKNTIGLMDAKDGEGIEA 420

BLAST of Lsi03G010480 vs. ExPASy TrEMBL
Match: A0A5D3E167 (Acylsugar acyltransferase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00870 PE=3 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 5.1e-112
Identity = 227/441 (51.47%), Postives = 297/441 (67.35%), Query Frame = 0

Query: 1   MMQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYEN-----GSNSKLLK 60
           MM+L IVSKETIKPL +TPP+L+   L LFD+ AP +Y+  LYFY N     G + +LLK
Sbjct: 1   MMELQIVSKETIKPLSATPPQLKKLPLSLFDQQAPKMYIHLLYFYYNHDISSGYSRQLLK 60

Query: 61  HSLSKVLTFYYPFAGRLKDD-CFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKLI 120
            SLSK LTFYYPFAGRL+DD   + CND G    EA+LRC MSE MN +NL  +E+LKL 
Sbjct: 61  DSLSKALTFYYPFAGRLRDDGNSINCNDVGAIVSEAKLRCPMSEFMNNFNLEREEILKLT 120

Query: 121 CFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSS-- 180
             DD+NGKDQR NPLLCVQLT+FECGGE + V  SHK+AD ++ TNF++ WA ++RSS  
Sbjct: 121 LSDDINGKDQRLNPLLCVQLTQFECGGEVMYVLCSHKVADLASLTNFINDWAFIARSSRD 180

Query: 181 --DMPPL-APPRFDAASLFEAAVADD----DSRSENLEKK---------NSSKIKALKAM 240
             D P L  PP+F+ AS F     +     +SR+E   +           SSKI +LK M
Sbjct: 181 GNDPPTLNCPPQFNGASFFPPTKLESGGELNSRTERQYENGVSTKRFVFKSSKIASLKGM 240

Query: 241 VSDKVENPSRVQILAAFIYKAAISA----------------------------SMVASNI 300
           V +KVENP+RVQIL AFIYKAA+SA                            + ++ N+
Sbjct: 241 VCEKVENPTRVQILTAFIYKAAVSAKESVTGSVPATILLQTINLRNRVDPPLPATLSGNV 300

Query: 301 ISFFLTSSTTAEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKESIE-K 360
           IS+F+  S T E +E++L +L+ D KRN EE+C  +P  Y++EEW  L +LH KES+E +
Sbjct: 301 ISWFIGLSRTQEKREMELWNLVGDTKRNFEEYCKTFPTDYRSEEWGLLQRLHAKESMEDR 360

Query: 361 MSNPKDQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVT 389
           M N +D+++Y CSSWC+FP Y+ADFG GKP WI+ P    KN+I+LMD+KDGEGIEA+V+
Sbjct: 361 MINEEDRSLYTCSSWCKFPIYEADFGRGKPGWISVPEFPWKNMILLMDSKDGEGIEAIVS 420

BLAST of Lsi03G010480 vs. ExPASy TrEMBL
Match: A0A1S3BZZ4 (acylsugar acyltransferase 3-like OS=Cucumis melo OX=3656 GN=LOC103494898 PE=3 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 5.1e-112
Identity = 227/441 (51.47%), Postives = 297/441 (67.35%), Query Frame = 0

Query: 1   MMQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYEN-----GSNSKLLK 60
           MM+L IVSKETIKPL +TPP+L+   L LFD+ AP +Y+  LYFY N     G + +LLK
Sbjct: 1   MMELQIVSKETIKPLSATPPQLKKLPLSLFDQQAPKMYIHLLYFYYNHDISSGYSRQLLK 60

Query: 61  HSLSKVLTFYYPFAGRLKDD-CFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKLI 120
            SLSK LTFYYPFAGRL+DD   + CND G    EA+LRC MSE MN +NL  +E+LKL 
Sbjct: 61  DSLSKALTFYYPFAGRLRDDGNSINCNDVGAIVSEAKLRCPMSEFMNNFNLEREEILKLT 120

Query: 121 CFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSS-- 180
             DD+NGKDQR NPLLCVQLT+FECGGE + V  SHK+AD ++ TNF++ WA ++RSS  
Sbjct: 121 LSDDINGKDQRLNPLLCVQLTQFECGGEVMYVLCSHKVADLASLTNFINDWAFIARSSRD 180

Query: 181 --DMPPL-APPRFDAASLFEAAVADD----DSRSENLEKK---------NSSKIKALKAM 240
             D P L  PP+F+ AS F     +     +SR+E   +           SSKI +LK M
Sbjct: 181 GNDPPTLNCPPQFNGASFFPPTKLESGGELNSRTERQYENGVSTKRFVFKSSKIASLKGM 240

Query: 241 VSDKVENPSRVQILAAFIYKAAISA----------------------------SMVASNI 300
           V +KVENP+RVQIL AFIYKAA+SA                            + ++ N+
Sbjct: 241 VCEKVENPTRVQILTAFIYKAAVSAKESVTGSVPATILLQTINLRNRVDPPLPATLSGNV 300

Query: 301 ISFFLTSSTTAEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKESIE-K 360
           IS+F+  S T E +E++L +L+ D KRN EE+C  +P  Y++EEW  L +LH KES+E +
Sbjct: 301 ISWFIGLSRTQEKREMELWNLVGDTKRNFEEYCKTFPTDYRSEEWGLLQRLHAKESMEDR 360

Query: 361 MSNPKDQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVT 389
           M N +D+++Y CSSWC+FP Y+ADFG GKP WI+ P    KN+I+LMD+KDGEGIEA+V+
Sbjct: 361 MINEEDRSLYTCSSWCKFPIYEADFGRGKPGWISVPEFPWKNMILLMDSKDGEGIEAIVS 420

BLAST of Lsi03G010480 vs. ExPASy TrEMBL
Match: A0A5A7TT14 (Acylsugar acyltransferase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00860 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 8.6e-112
Identity = 226/441 (51.25%), Postives = 297/441 (67.35%), Query Frame = 0

Query: 1   MMQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYEN-----GSNSKLLK 60
           MM+L IVSKETIKPL +TPP+L+   L LFD+ AP +Y+  LYFY N     G + +LLK
Sbjct: 1   MMELQIVSKETIKPLSATPPQLKKLPLSLFDQQAPKMYIHLLYFYYNHDISSGYSRQLLK 60

Query: 61  HSLSKVLTFYYPFAGRLKDD-CFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKLI 120
            SLSK LTFYYPFAGRL+DD   + CND G    EA+LRC MSE MN +NL  +E+LKL 
Sbjct: 61  DSLSKALTFYYPFAGRLRDDGNSINCNDVGAIVSEAKLRCPMSEFMNNFNLEREEILKLT 120

Query: 121 CFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSS-- 180
             DD+NGKDQR NPLLCVQLT+FECGGE + V  SHK+AD ++ TNF++ W  ++RSS  
Sbjct: 121 LSDDINGKDQRLNPLLCVQLTQFECGGEVMYVLCSHKVADLASLTNFINDWVFIARSSRD 180

Query: 181 --DMPPL-APPRFDAASLFEAAVADD----DSRSENLEKK---------NSSKIKALKAM 240
             D P L  PP+F+ AS F     +     +SR+E   +           SSKI +LK M
Sbjct: 181 GNDPPTLNCPPQFNGASFFPPTKLESGGELNSRTERQYENGVSTKRFVFKSSKIASLKGM 240

Query: 241 VSDKVENPSRVQILAAFIYKAAISA----------------------------SMVASNI 300
           V +KVENP+RVQIL AFIYKAA+SA                            + ++ N+
Sbjct: 241 VCEKVENPTRVQILTAFIYKAAVSAKESVTGSVPATILLQTINLRNRVDPPLPATLSGNV 300

Query: 301 ISFFLTSSTTAEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKESIE-K 360
           IS+F+  S T E +E++L +L+ D KRN EE+C  +P +Y++EEW  L +LH KES+E +
Sbjct: 301 ISWFIGLSRTQEKREMELWNLVGDTKRNFEEYCKTFPTNYRSEEWGLLQRLHAKESMEDR 360

Query: 361 MSNPKDQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVT 389
           M N +D+++Y CSSWC+FP Y+ADFG GKP WI+ P    KN+I+LMD+KDGEGIEA+V+
Sbjct: 361 MINEEDRSLYTCSSWCKFPIYEADFGRGKPGWISVPEFPWKNMILLMDSKDGEGIEAIVS 420

BLAST of Lsi03G010480 vs. NCBI nr
Match: XP_031740025.1 (stemmadenine O-acetyltransferase isoform X2 [Cucumis sativus])

HSP 1 Score: 620.9 bits (1600), Expect = 7.3e-174
Identity = 322/439 (73.35%), Postives = 351/439 (79.95%), Query Frame = 0

Query: 1   MMQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYENGSNSKLLKHSLSK 60
           MMQL IVSKETIKPLFSTPPEL+LHHLCLFDRHAPYLYL FLYFY+N S+SKLLKHSLSK
Sbjct: 1   MMQLEIVSKETIKPLFSTPPELKLHHLCLFDRHAPYLYLHFLYFYQNSSHSKLLKHSLSK 60

Query: 61  VLTFYYPFAGRLKDDCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKLICFDDMN 120
            LTFYYPFAGRLKDDC ++CND G T LEA LRC MSE MNVYNLRHDE LKL+CFDDMN
Sbjct: 61  ALTFYYPFAGRLKDDCSIDCNDMGATILEARLRCPMSEFMNVYNLRHDEALKLVCFDDMN 120

Query: 121 --GKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSS--DMPP 180
              KDQRYNPLLCVQLTRFECGGE +CVFLSHKLADASTFTNFM+HWAS+SR+   DMP 
Sbjct: 121 DGSKDQRYNPLLCVQLTRFECGGEVLCVFLSHKLADASTFTNFMNHWASLSRTGDHDMPL 180

Query: 181 LAPPRFDAASLFEAAVADD------DSRSENLEKK----NSSKIKALKAMVSDKVENPSR 240
           L PPRFDA SLFEAA  DD        R +NL  K     +SKI ALKA V DKVENPSR
Sbjct: 181 LPPPRFDAGSLFEAATVDDVLGSKIVKREKNLVSKRMVFKASKIAALKATVCDKVENPSR 240

Query: 241 VQILAAFIYKAAISAS----------------------------------MVASNIISFF 300
           VQILAAFIYKAAISAS                                   ++ NIISFF
Sbjct: 241 VQILAAFIYKAAISASKSTLSIHHSRTPITNYLLRIAINLRTRLIPPMPATLSGNIISFF 300

Query: 301 LTS-STTAEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKESIEKMSNP 360
           LTS +TTAE KEI+LSSLL+DMKRNM+EFCNKYP++YKAEEWS LYKLH+KES+E+M+NP
Sbjct: 301 LTSATTTAEQKEIELSSLLSDMKRNMKEFCNKYPRNYKAEEWSSLYKLHIKESMERMNNP 360

Query: 361 KDQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVTLEKE 391
           +DQ VYACSSWCRFPFYDADFGWGKP+WIT P ILSKNVIILMDAKDGEGIEA V+LE+E
Sbjct: 361 EDQIVYACSSWCRFPFYDADFGWGKPVWITTPTILSKNVIILMDAKDGEGIEAFVSLEEE 420

BLAST of Lsi03G010480 vs. NCBI nr
Match: XP_004152371.2 (stemmadenine O-acetyltransferase isoform X1 [Cucumis sativus] >KGN52789.1 hypothetical protein Csa_015235 [Cucumis sativus])

HSP 1 Score: 620.2 bits (1598), Expect = 1.3e-173
Identity = 322/441 (73.02%), Postives = 351/441 (79.59%), Query Frame = 0

Query: 1   MMQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYENGSNSKLLKHSLSK 60
           MMQL IVSKETIKPLFSTPPEL+LHHLCLFDRHAPYLYL FLYFY+N S+SKLLKHSLSK
Sbjct: 1   MMQLEIVSKETIKPLFSTPPELKLHHLCLFDRHAPYLYLHFLYFYQNSSHSKLLKHSLSK 60

Query: 61  VLTFYYPFAGRLKDDCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKLICFDDMN 120
            LTFYYPFAGRLKDDC ++CND G T LEA LRC MSE MNVYNLRHDE LKL+CFDDMN
Sbjct: 61  ALTFYYPFAGRLKDDCSIDCNDMGATILEARLRCPMSEFMNVYNLRHDEALKLVCFDDMN 120

Query: 121 --GKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSS--DMPP 180
              KDQRYNPLLCVQLTRFECGGE +CVFLSHKLADASTFTNFM+HWAS+SR+   DMP 
Sbjct: 121 DGSKDQRYNPLLCVQLTRFECGGEVLCVFLSHKLADASTFTNFMNHWASLSRTGDHDMPL 180

Query: 181 LAPPRFDAASLFEAAVADD--------DSRSENLEKK----NSSKIKALKAMVSDKVENP 240
           L PPRFDA SLFEAA  DD          R +NL  K     +SKI ALKA V DKVENP
Sbjct: 181 LPPPRFDAGSLFEAATVDDVLGEGSKIVKREKNLVSKRMVFKASKIAALKATVCDKVENP 240

Query: 241 SRVQILAAFIYKAAISAS----------------------------------MVASNIIS 300
           SRVQILAAFIYKAAISAS                                   ++ NIIS
Sbjct: 241 SRVQILAAFIYKAAISASKSTLSIHHSRTPITNYLLRIAINLRTRLIPPMPATLSGNIIS 300

Query: 301 FFLTS-STTAEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKESIEKMS 360
           FFLTS +TTAE KEI+LSSLL+DMKRNM+EFCNKYP++YKAEEWS LYKLH+KES+E+M+
Sbjct: 301 FFLTSATTTAEQKEIELSSLLSDMKRNMKEFCNKYPRNYKAEEWSSLYKLHIKESMERMN 360

Query: 361 NPKDQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVTLE 391
           NP+DQ VYACSSWCRFPFYDADFGWGKP+WIT P ILSKNVIILMDAKDGEGIEA V+LE
Sbjct: 361 NPEDQIVYACSSWCRFPFYDADFGWGKPVWITTPTILSKNVIILMDAKDGEGIEAFVSLE 420

BLAST of Lsi03G010480 vs. NCBI nr
Match: XP_038905756.1 (BAHD acyltransferase BIA1-like [Benincasa hispida])

HSP 1 Score: 429.5 bits (1103), Expect = 3.1e-116
Identity = 239/454 (52.64%), Postives = 303/454 (66.74%), Query Frame = 0

Query: 2   MQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYE--NGSNS---KLLKH 61
           M+L IVSKETIKPL STPPEL+   L LFD+ AP +Y++ LYFY   N S+S   +LLK 
Sbjct: 1   MELEIVSKETIKPLSSTPPELKNLPLSLFDQQAPNMYIRLLYFYNSYNISSSHGRQLLKD 60

Query: 62  SLSKVLTFYYPFAGRLKD-DCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKLIC 121
           SLSK LTFYYPFAGRL+D    ++CND G    EA+LRC MSE MN +N+  +E+LKL  
Sbjct: 61  SLSKALTFYYPFAGRLQDGGNSIDCNDMGAIVSEAKLRCPMSEFMNNFNMECEEILKLTF 120

Query: 122 FDDMN-GKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSS-- 181
           FD++N G DQR NPLLCVQLT+FECGGE + V  SHK+AD ++ TNFM  WA ++RSS  
Sbjct: 121 FDEINDGNDQRLNPLLCVQLTQFECGGEVMYVLCSHKIADFASLTNFMKDWAFIARSSGD 180

Query: 182 -DMPPL-APPRFDAASLFEAAV---ADDDSRSENLEKK---------NSSKIKALKAMVS 241
            D P L +PP+F+ AS F   +     +DSR+E  ++            SKI ALK+MV 
Sbjct: 181 GDPPTLSSPPQFNGASFFPLELDGGGGEDSRTERHDENVVSSKRFVFEGSKIAALKSMVC 240

Query: 242 DKVENPSRVQILAAFIYKAAISA------------------------------------- 301
           +KVENP+RVQ+L AFIYKAA+SA                                     
Sbjct: 241 EKVENPTRVQLLTAFIYKAALSAKNSVTGVLILVCIIFKLYHSYVPATTLLQLINLRNRV 300

Query: 302 -----SMVASNIISFFLTSSTTAEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCL 361
                + ++ NIIS F+ S T  E +E++L  L+ DMKRN EEFC  +P +Y+AE+W  L
Sbjct: 301 DPPLPATLSGNIISSFIASLTMPEQQEMELWDLVGDMKRNFEEFCKTFPTNYRAEQWGLL 360

Query: 362 YKLHMKESIEKMSNPKDQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDA 391
           YKLH KES+EK+ NP D T+Y CSSWC+FP YD DFGWGKP+WIT P    KN+I+LMD+
Sbjct: 361 YKLHAKESLEKVRNPGDCTLYTCSSWCKFPVYDVDFGWGKPVWITVPEFPWKNMILLMDS 420

BLAST of Lsi03G010480 vs. NCBI nr
Match: KAG6581251.1 (Tabersonine-19-hydroxy-O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 427.2 bits (1097), Expect = 1.6e-115
Identity = 236/446 (52.91%), Postives = 289/446 (64.80%), Query Frame = 0

Query: 2   MQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYEN--------GSNSKL 61
           M+L IVSKETIKPL STPP+L    L  FD+H P +Y   LYFY N        G   +L
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCRGRL 60

Query: 62  LKHSLSKVLTFYYPFAGRLKD-DCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLK 121
           LK SLSK LTFYYPFAGRL+D   F++CND G TF+EA+LRC MSE+MN ++L HDE+LK
Sbjct: 61  LKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFVEAKLRCPMSEVMNTFDLNHDEILK 120

Query: 122 LICFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSS 181
           L+CF+D+ GK Q ++PLL VQLT+FECGGE + V L+HK+AD +TF NFM+ WAS++RSS
Sbjct: 121 LLCFEDIKGKVQTFDPLLSVQLTQFECGGEVMYVSLAHKVADLATFANFMNDWASIARSS 180

Query: 182 D--MPPLAPPRFDAASLF-----------EAAVADDDSRSENLEKK----NSSKIKALKA 241
               PP+  P F AASLF           E   +    R EN+  +      SKI+ALKA
Sbjct: 181 GGIDPPVVCPLFHAASLFRPELDGGVDPSEGEGSGSKKREENVRSRRMVFEGSKIRALKA 240

Query: 242 MVSDKVENPSRVQILAAFIYKAAISASMVAS----------------------------- 301
           MVS KVENP+RVQ+L AFIYKA +SA    S                             
Sbjct: 241 MVSKKVENPTRVQVLTAFIYKAIVSAKNALSGGLSREPTSILQFINLRSRVEPPLPGTLT 300

Query: 302 -NIISFFLTSSTTAE-HKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKES 361
            NI+SFF  SST+AE  +E++L S++ DMKRN EEFC K P++Y+ EE S   K  +KES
Sbjct: 301 GNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEEFCRKLPRNYRDEEGSSALKSWIKES 360

Query: 362 IEKMSNPKDQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEA 391
            E      D   Y CSSWCRFP Y+ADFGWG P+WI  P  LSKN I LMDAKDGEGIEA
Sbjct: 361 FETWMMDNDGRKYGCSSWCRFPLYEADFGWGTPIWIAVPAFLSKNTIGLMDAKDGEGIEA 420

BLAST of Lsi03G010480 vs. NCBI nr
Match: XP_022935661.1 (vinorine synthase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 426.4 bits (1095), Expect = 2.7e-115
Identity = 236/446 (52.91%), Postives = 289/446 (64.80%), Query Frame = 0

Query: 2   MQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYEN--------GSNSKL 61
           M+L IVSKETIKPL STPP+L    L  FD+H P +Y   LYFY N        G   +L
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCRGRL 60

Query: 62  LKHSLSKVLTFYYPFAGRLKD-DCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLK 121
           LK SLSK LTFYYPFAGRL+D   F++CND G TF+EA+LRC MSE+MN ++L HDE+LK
Sbjct: 61  LKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFVEAKLRCPMSEVMNTFDLNHDEILK 120

Query: 122 LICFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSS 181
           L+CF+D+ GK Q ++PLL VQLT+FECGGE + V L+HK+AD +TF NFM+ WAS++RSS
Sbjct: 121 LLCFEDIKGKVQTFDPLLSVQLTQFECGGEVMYVSLAHKVADLATFANFMNDWASIARSS 180

Query: 182 D--MPPLAPPRFDAASLF-----------EAAVADDDSRSENLEKK----NSSKIKALKA 241
               PP+  P F AASLF           E   +    R EN+  +      SKI+ALKA
Sbjct: 181 GGIDPPVVCPLFHAASLFRPELDGGVDPSEGEGSGSKKREENVRSRRMVFEGSKIRALKA 240

Query: 242 MVSDKVENPSRVQILAAFIYKAAISASMVAS----------------------------- 301
           MVS KVENP+RVQ+L AFIYKA +SA    S                             
Sbjct: 241 MVSKKVENPTRVQVLTAFIYKAIVSAKNALSGGLSREPTSILQFINLRSRVEPPLPGTLT 300

Query: 302 -NIISFFLTSSTTAE-HKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKES 361
            NI+SFF  SST+AE  +E++L S++ DMKRN EEFC K P++Y+ EE S   K  +KES
Sbjct: 301 GNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEEFCRKLPRNYRDEEGSPALKSWIKES 360

Query: 362 IEKMSNPKDQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEA 391
            E      D   Y CSSWCRFP Y+ADFGWG P+WI  P  LSKN I LMDAKDGEGIEA
Sbjct: 361 FETWMMDNDGRKYGCSSWCRFPLYEADFGWGTPIWIAVPAFLSKNTIGLMDAKDGEGIEA 420

BLAST of Lsi03G010480 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 190.3 bits (482), Expect = 3.0e-48
Identity = 136/441 (30.84%), Postives = 226/441 (51.25%), Query Frame = 0

Query: 2   MQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYENGSNSK------LLK 61
           M++ +VS++ IKP   TP  L+   L L ++  P ++   ++FY   ++ K      +LK
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQLQMLK 60

Query: 62  HSLSKVLTFYYPFAGRLKDDCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKLI- 121
            SLS+ LT +YP AGRLK +  ++CND+G  FLEA +   +S ++       D + +LI 
Sbjct: 61  KSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLL--LEPSSDSLQQLIP 120

Query: 122 -CFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSSD 181
              D +  + +    LL  Q + FECG  +I V +SHKLADA++   FM  WA++S    
Sbjct: 121 TSVDSIETRTR----LLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGS 180

Query: 182 MPPLAPPRFDAASLF----------EAAVADDDSRSENLEKK---NSSKIKALKAMVSD- 241
           +  +  P FD   +F             V  +   ++ L K+   +SS I+AL+A  S  
Sbjct: 181 IKTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKASSF 240

Query: 242 KVENPSRVQILAAFIYKAAISASMVAS----------------------------NIISF 301
           +V  P+RV+ ++A I+K+A+ A+   S                            N++S+
Sbjct: 241 EVNQPTRVEAVSALIWKSAMKATRTVSGTSKPSILANSVSLRSRVSPPFTKNSIGNLVSY 300

Query: 302 FLTSSTTAEHKEIDLSSLLNDMKRNMEEFCN----KYPKHYKAEEWSCLYKLHMKESIEK 361
           F   +    + +  L +L++ +++  + F +    K   +  A E  C Y+   KE+ + 
Sbjct: 301 FAAKAEEGIN-QTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQ---KEAGDM 360

Query: 362 MSNPKDQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVT 389
           +++  D   Y  SS CRF  Y+ DFGWGKP+W+  P +  KN++ L+D K+  GIEA V 
Sbjct: 361 IAS-GDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIEAWVN 420

BLAST of Lsi03G010480 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.6e-44
Identity = 133/433 (30.72%), Postives = 223/433 (51.50%), Query Frame = 0

Query: 2   MQLAIVSKETIKPL---FSTPPELRLHHLCLFDRHAPYLYLQFLYFYENGSN------SK 61
           +++ + S+E +KP     + PP    HHL   D+ AP +++ FL+FY N +N      S 
Sbjct: 3   LEITVTSQELVKPSPRNLNHPP--CHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKERSD 62

Query: 62  LLKHSLSKVLTFYYPFAGRLKDD-CFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVL 121
            +K SLS++L  YYP AGR+K+    V CND GV+F+EA+  C+MS+I+   N   +E+ 
Sbjct: 63  HIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILE--NPNPNELN 122

Query: 122 KLICFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRS 181
           KL  F+     D      L VQLT FECGG A+ + LSHKL DA +   F++ WA+ +R 
Sbjct: 123 KLHPFEFHEVSDVP----LTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFAR- 182

Query: 182 SDMPPLAPPRFDAASLFEAAVADDDSRSENLEKKN---------SSKIKALKAMVS-DKV 241
                +  P FD A +F     ++ + +  + K+N          S +++L+   S +K 
Sbjct: 183 GQTDEIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRERFSGNKK 242

Query: 242 ENPSRVQILAAFIYKAAISAS----------------------------MVASNIISFFL 301
              +RV++L+ FI+   ++++                             +  NI+ F +
Sbjct: 243 IRATRVEVLSVFIWSRFMASTNHDDKTGKIYTLIHPVNLRRQADPDIPDNMFGNIMRFSV 302

Query: 302 T-SSTTAEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKESIEKMSNPK 361
           T         + + +SL++ M+  + +    Y K  + +    L  L+ + S     N  
Sbjct: 303 TVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQAS--GFVN-G 362

Query: 362 DQTVYACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVTLEKEE 386
           +   ++ +S C+FP Y+ADFGWGKPLW+ +  +  KN++  +D K+G+GIEA + L++ +
Sbjct: 363 EIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWINLDQND 422

BLAST of Lsi03G010480 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 176.4 bits (446), Expect = 4.5e-44
Identity = 133/437 (30.43%), Postives = 225/437 (51.49%), Query Frame = 0

Query: 2   MQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYENG------SNSKLLK 61
           M++  ++KE IKP  +TP +L+   L ++D   P +Y     FY          +S  LK
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQSSHKLK 63

Query: 62  HSLSKVLTFYYPFAGRLKDDCFVECNDTGVTFLEAEL-RCSMSEIMNVYNLRH-DEVLKL 121
            SL++ LT +YP AGR+K    ++CND G  F++A +    +S+ +   + +   ++L L
Sbjct: 64  TSLAETLTKFYPLAGRIK-GVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQLLPL 123

Query: 122 ICFDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSSD 181
              DD       + PLL V+ T F CGG AI + ++HK+ADA++ + F+  WA+++R   
Sbjct: 124 DVIDDPYEAATTW-PLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMARGEA 183

Query: 182 MPPLAPPRFDAAS-------LFEAAVADDDSRSENLEKK---NSSKIKAL--KAMVSDKV 241
              +A P F AA+       LF+  V ++ ++   + K+   ++SK++ L  KA   D V
Sbjct: 184 GDIVADPVFAAANFYPPANELFKFPVDENANKISQITKRFVFSASKLEELRTKAASEDFV 243

Query: 242 ENPSRVQILAAFIYKAAISA------------------------SMVASNIISFFLTSST 301
             P RV+ + A ++KA ++A                        S++A ++I   + SS 
Sbjct: 244 GRPKRVESVTALLWKAFVAAASSNTNACDMKVLIQPSNLRPKIPSLLAESLIGNIMFSSV 303

Query: 302 T---AEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLH--MKESIEKMSNPK 361
               ++ +EI +   + D+++  ++  N   +   +       KL   M  +  K+S   
Sbjct: 304 VLGISQQEEIKIEKAVRDLRKQGDDLQNVIKEEGGSSSAMIGSKLANLMLTNYSKLSYET 363

Query: 362 DQTVYACSSWCRFPFYDADFGWGKPLWITAPVI-LSKNVIILMDAKDGEGIEALVTLEKE 389
            +  Y  SSWC+ P Y+A FGWG P+WI   V  + +NV +L+D+KDG+GIEA VTL +E
Sbjct: 364 HEP-YTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDSKDGQGIEAFVTLPEE 423

BLAST of Lsi03G010480 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 168.3 bits (425), Expect = 1.2e-41
Identity = 131/435 (30.11%), Postives = 214/435 (49.20%), Query Frame = 0

Query: 6   IVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFY----------ENGSNSKLLK 65
           I+S+E +KP   TP + R+ +L L D  +  +Y   L FY               S  LK
Sbjct: 7   ILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETSLKLK 66

Query: 66  HSLSKVLTFYYPFAGRLKDDCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKLIC 125
            SLSK L  +YP AGR+    FVECND G  F+EA +   +SE +        E L+L+ 
Sbjct: 67  KSLSKTLPIFYPLAGRIIGS-FVECNDEGAVFIEARVDHLLSEFL---KCPVPESLELLI 126

Query: 126 FDDMNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSSDMP 185
             +   ++    P+L +Q   F CGG  I + +SHK+ DA++   F+  WA  SR   + 
Sbjct: 127 PVEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRGLGIT 186

Query: 186 PLAPPRFDAASLFEAAVADDDS----RSENLEKK---------NSSKIKALKAMVS-DKV 245
            +  P F A+ +F   + +  S    R E +E+          ++SKIK L+A  S + V
Sbjct: 187 LI--PSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAKASRNLV 246

Query: 246 ENPSRVQILAAFIYKAAISASMVAS---------------------------NIISFFLT 305
           +NP+RV+ + A  ++     S ++S                           N++S  + 
Sbjct: 247 KNPTRVEAVTALFWRCVTKVSRLSSLTPRTSVLQILVNLRGKVDSLCENTIGNMLSLMIL 306

Query: 306 SSTTAEHKEIDLSSLLNDMKRNMEEFCNKYPKHYKAEEWSCLYKLHMKESIEKMSNPKDQ 365
            +  A  + I    ++++++R  E F     +  K+   S +++L ++E  +      + 
Sbjct: 307 KNEEAAIERI--QDVVDEIRRAKEIFSLNCKEMSKSS--SRIFEL-LEEIGKVYGRGNEM 366

Query: 366 TVYACSSWCRFPFYDADFGWGKPLWITA-PVILSKNVIILMDAKDGEGIEALVTLEKEEM 389
            ++  +SWC+   YDADFGWGKP+W+T       KN+++L+D KDGEGIEA +TL +E+M
Sbjct: 367 DLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEGIEAWITLTEEQM 426

BLAST of Lsi03G010480 vs. TAIR 10
Match: AT5G23970.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 160.6 bits (405), Expect = 2.5e-39
Identity = 132/429 (30.77%), Postives = 204/429 (47.55%), Query Frame = 0

Query: 2   MQLAIVSKETIKPLFSTPPELRLHHLCLFDRHAPYLYLQFLYFYENGSNS---KLLKHSL 61
           M+L ++SKE IKP  ++P  L+   L LFD+  P  Y+  ++FY++ SN    + LK SL
Sbjct: 1   MKLELLSKEVIKP--ASPNHLQTLSLSLFDQFLPSTYVSAIFFYDDHSNQEDIQRLKSSL 60

Query: 62  SKVLTFYYPFAGRLKDDCFVECNDTGVTFLEAELRCSMSEIMNVYNLRHDEVLKLICFDD 121
           S+ L+ +YP AG++KD   V CND G  F EA     +S+ +   +   D + K I   D
Sbjct: 61  SQTLSLFYPLAGQIKDGVTVHCNDEGALFTEARAEIFLSDFLRNPS-DADLIQKFIVSPD 120

Query: 122 MNGKDQRYNPLLCVQLTRFECGGEAICVFLSHKLADASTFTNFMSHWASVSRSSDMPPLA 181
               D    PLL V++  F+  G A+ V +SHK+ DA++ + F+  W   S+        
Sbjct: 121 --HADPETWPLLHVKVIFFKDKGFAVAVSVSHKICDAASLSTFVCSWTKASKG--YADTV 180

Query: 182 PPRFDAASLFEAA----------VADDDSRSENLEKKNSSKIKALKAMVSD--KVENPSR 241
            P F  A  +  A          V +  S+++      S  I+ LK   S   +V   +R
Sbjct: 181 DPEFVGADFYPPADISIEFPPLLVHETKSKTKRF-VFGSLMIEKLKNRASSGKRVPQATR 240

Query: 242 VQILAAFIYKAAISASMVASNIISFFL----------TSSTTAEHKEI------DLSSLL 301
           ++ + A + +    A    S  +  F            SS+   HK I       L    
Sbjct: 241 IESITALLLRCMTKAGHSKSGKVKEFAITQTMDLRPRVSSSLLPHKAIGNFFFLPLLKES 300

Query: 302 NDMKRNMEEFCNKYPKH-------YKAEEWSCLYKLHMKESIEKMS-------NPKDQTV 361
           ++ K  +EE  +K  K         + +       +  KE I           +P+ +T 
Sbjct: 301 SESKMEIEETVSKLQKTKQELNELIRNDSEDAKSSVEAKERIASAMLSSLCEISPEMET- 360

Query: 362 YACSSWCRFPFYDADFGWGKPLWITAPVILSKNVIILMDAKDGEGIEALVTLEKEEMEVF 386
           YA SSWCR  FY+A+FGWGKP+W+ AP  + K  ++LMD+KD EG+EA VTL + +M  +
Sbjct: 361 YAVSSWCRMSFYEANFGWGKPVWV-APDSVDKTQVVLMDSKDSEGVEARVTLPETDMAKY 419

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A2G3D6U15.0e-4832.09Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1[more]
Q58VT11.4e-4732.33Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1[more]
D2Y3X21.9e-4732.09Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1[more]
K4D9Y41.1e-4229.37Acylsugar acyltransferase 3 OS=Solanum lycopersicum OX=4081 GN=ASAT3 PE=2 SV=1[more]
A0A2P1GIW75.3e-4229.55Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KV476.1e-17473.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G001560 PE=3 SV=1[more]
A0A6J1F5C11.3e-11552.91vinorine synthase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442451 ... [more]
A0A5D3E1675.1e-11251.47Acylsugar acyltransferase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BZZ45.1e-11251.47acylsugar acyltransferase 3-like OS=Cucumis melo OX=3656 GN=LOC103494898 PE=3 SV... [more]
A0A5A7TT148.6e-11251.25Acylsugar acyltransferase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
XP_031740025.17.3e-17473.35stemmadenine O-acetyltransferase isoform X2 [Cucumis sativus][more]
XP_004152371.21.3e-17373.02stemmadenine O-acetyltransferase isoform X1 [Cucumis sativus] >KGN52789.1 hypoth... [more]
XP_038905756.13.1e-11652.64BAHD acyltransferase BIA1-like [Benincasa hispida][more]
KAG6581251.11.6e-11552.91Tabersonine-19-hydroxy-O-acetyltransferase, partial [Cucurbita argyrosperma subs... [more]
XP_022935661.12.7e-11552.91vinorine synthase-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G26040.13.0e-4830.84HXXXD-type acyl-transferase family protein [more]
AT1G24430.12.6e-4430.72HXXXD-type acyl-transferase family protein [more]
AT3G30280.14.5e-4430.43HXXXD-type acyl-transferase family protein [more]
AT1G24420.11.2e-4130.11HXXXD-type acyl-transferase family protein [more]
AT5G23970.12.5e-3930.77HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..197
e-value: 1.0E-51
score: 177.3
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 205..385
e-value: 2.1E-33
score: 117.3
IPR003480TransferasePFAMPF02458Transferasecoord: 247..382
e-value: 2.2E-20
score: 72.7
coord: 3..189
e-value: 8.7E-36
score: 123.5
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 3..245
coord: 244..387
NoneNo IPR availablePANTHERPTHR31623:SF51N-BENZOYLTRANSFERASE, PUTATIVE-RELATEDcoord: 3..245
coord: 244..387

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G010480.1Lsi03G010480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups