Lsi03G010350 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G010350
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMediator of RNA polymerase II transcription subunit 28
Locationchr03: 19907092 .. 19909856 (+)
RNA-Seq ExpressionLsi03G010350
SyntenyLsi03G010350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCATTATGTTCATGGAGTTACAATATGATGTTGGTTTTGGTTCTGTTTGTTGCTCATATGTTGATGGTACTCTAAATAAGTACATCCCTGATGCCTATAGAGTTGTTTTATGTTTTGATTAGTATATTTTTCCAAGAATTGAAATGGAGTTTTGATTGTTGTAAAAGGATATGGCTGAACGACAGCCACTCGACCAACAACATCCACTTGATCCACAATCGCAATCATCACAACCTCCAAGAGAAGATATGATTGCATATGTGATGGCTTTGGAAGCTGCTCTGCTTCCATGCTTGCCTGCAAGAGAACTACAAGCAATAGACCGTTCTCCTCACCCTTCTCATCAAGGTCAGTAACTGATCCTTGTGTAATTAGATTCTGAAAAAACATTGTTAAACTAGTGTTTGGGCGGTTACCTTTATACATTATTGTATCTTCTACCCTACCATGTTTCTCCATATGATATGGTCTCTTGAATCAAATAAGTTGTTTTTTCTTTTCTTTTTTGGGGTGCAATTGTGCTTAATAACAATTTTGCATGAATGTTAATCTAAAGTTTCGGTCACTATGGGTCTTCATAGTGCGAGCTCTTTGTTTTTGTTGTTATTTTATTTACTATTCATGATGGTTTCGCAGTTTTTTATTATTGACTAGAAAAATAAAGTGTTGCATAATGGTTATTGGACCGTGTTATAATTAATTAGGATGAAGAGCAACTAGTAGATTTTATTTTATTTTGTTTTTTTTTAGAAGAGAAACTTGTAGATGCAGCAAACAATAAATTGGTGCAAAGAGTGACAGTTCATTGACCATTGTTAACTTTAAAGACAGGGGGTGGGTTGGATGCTGAACCGGTGTTTGAGTTGTAGATTTGTCAAGCATGAGGTTTGTAAGAATAACCCATGGGTTTTGACATTATCCTAAGCACCACCCCAGATCTATTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGCAAACACACTCCCCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGGGGGGGGGGGTGGGGTGGTCCTAAACCTGCACCCCGAACAAACTTGTTAAATCCTCCACTTAAACGTTTGGCTTAATATTTGTAGGTTTAAGATGCCAAGCCTATGCCTCAAATGACCCTCAAGACTATTCTTGAGTTTCTTTCTTATTGAAGGAGAGCCAAAGGAGGCCTACACTAGCATTTGTCCCAGAACTTTAACTATGTTTAAACTACCAGCCTACAGATCAATTCTAAAATAGTCAACTATTGTTGTCCCCTGCTTTGTATTAGAAACGATAATGTAATTACACAATTGGTTGCATACGAATGAGTGCTACCTTGCTTGCAGCATTGAGCACAGTTCCCAGACCCTATGCAAAAACAAATTCCCCGGTGATTGGATGTTTTATGTGGATGTTTCCATTAATTTGTTGTAATTATGTGGAGTGGAAGTGACGTTTAGATCTAGAAGTAGCCATAAGTGTCTGCAATCTGCAACTCACTTAGGCAAGCCGAGCATTGAACTGATATGTCTGTGGTTAGGTGTTTGTAAACAGTGAAGAAAATGATAACCTGTTTTGTTATTGCTTCAGTTGACGTGGAAAGACATGCTAGGGATTTCATGGAGGCAGCTAAAAAGCTTCAATTGTATTTCATTGGTTTACAACGCGAGGATCAACCAACCAAAGTGGAAACACTAAGAAAGGTAAGCACTTTTTTGTTTTTTCCAGTTCAAAGAGGGACTCTCTTTTATGATAAATGTAGCTATTCTTTTTATGTAATATTTGTCATACTTCCCATCAGGAGATATCTGCCATTGAGGAGGAATTAAAGTTAAAGAATGACATAATCAAGAAGCAGGAGAGGCTCATTGAAGGGTGGAAGAAGGATTTGAAAGACCAGTTAGACAAACATAACAATGAGTTAGAGAAGGTTTAATAGAATAGGCTTGCGATCCCATGATCTATTGTTTAGGTATTTGTAGATCTTGTCAATTTATATACTTTCACAATTAGTTTATGGTGGGTATTTGTAGATCTTGTCAATTTATATACTTTCACAATTAGTTTATGGTGGGGCTATTGTTTGCTTATATTTTTAATGATCATTCTCTCCTTTGTTGGATCTCTTACGAATCTATTTTTCATATTGAATAGAGAAACGTGGGAAATTTTAGAATAATGTGGTCGATTGACCTAAAAGATTTGGTCACAATAA

mRNA sequence

ATGCCATTATGTTCATGGAGTTACAATATGATGTTGGTTTTGGTTCTGTTTGTTGCTCATATGTTGATGGATATGGCTGAACGACAGCCACTCGACCAACAACATCCACTTGATCCACAATCGCAATCATCACAACCTCCAAGAGAAGATATGATTGCATATGTGATGGCTTTGGAAGCTGCTCTGCTTCCATGCTTGCCTGCAAGAGAACTACAAGCAATAGACCGTTCTCCTCACCCTTCTCATCAAGTTGACGTGGAAAGACATGCTAGGGATTTCATGGAGGCAGCTAAAAAGCTTCAATTGTATTTCATTGGTTTACAACGCGAGGATCAACCAACCAAAGTGGAAACACTAAGAAAGGAGATATCTGCCATTGAGGAGGAATTAAAGTTAAAGAATGACATAATCAAGAAGCAGGAGAGGCTCATTGAAGGGTGGAAGAAGGATTTGAAAGACCAGTTAGACAAACATAACAATGAGTTAGAGAAGGTTTAATAGAATAGGCTTGCGATCCCATGATCTATTGTTTAGGTATTTGTAGATCTTGTCAATTTATATACTTTCACAATTAGTTTATGGTGGGTATTTGTAGATCTTGTCAATTTATATACTTTCACAATTAGTTTATGGTGGGGCTATTGTTTGCTTATATTTTTAATGATCATTCTCTCCTTTGTTGGATCTCTTACGAATCTATTTTTCATATTGAATAGAGAAACGTGGGAAATTTTAGAATAATGTGGTCGATTGACCTAAAAGATTTGGTCACAATAA

Coding sequence (CDS)

ATGCCATTATGTTCATGGAGTTACAATATGATGTTGGTTTTGGTTCTGTTTGTTGCTCATATGTTGATGGATATGGCTGAACGACAGCCACTCGACCAACAACATCCACTTGATCCACAATCGCAATCATCACAACCTCCAAGAGAAGATATGATTGCATATGTGATGGCTTTGGAAGCTGCTCTGCTTCCATGCTTGCCTGCAAGAGAACTACAAGCAATAGACCGTTCTCCTCACCCTTCTCATCAAGTTGACGTGGAAAGACATGCTAGGGATTTCATGGAGGCAGCTAAAAAGCTTCAATTGTATTTCATTGGTTTACAACGCGAGGATCAACCAACCAAAGTGGAAACACTAAGAAAGGAGATATCTGCCATTGAGGAGGAATTAAAGTTAAAGAATGACATAATCAAGAAGCAGGAGAGGCTCATTGAAGGGTGGAAGAAGGATTTGAAAGACCAGTTAGACAAACATAACAATGAGTTAGAGAAGGTTTAA

Protein sequence

MPLCSWSYNMMLVLVLFVAHMLMDMAERQPLDQQHPLDPQSQSSQPPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKNDIIKKQERLIEGWKKDLKDQLDKHNNELEKV
Homology
BLAST of Lsi03G010350 vs. ExPASy Swiss-Prot
Match: Q9LFA5 (Mediator of RNA polymerase II transcription subunit 28 OS=Arabidopsis thaliana OX=3702 GN=MED28 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.0e-38
Identity = 82/118 (69.49%), Postives = 105/118 (88.98%), Query Frame = 0

Query: 49  EDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQ 108
           ED++A V ALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYF+GL+
Sbjct: 39  EDIMACVTALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFMGLK 98

Query: 109 REDQ-PTKVETLRKEISAIEEELKLKNDIIKKQERLIEGWKKDLKDQLDKHNNELEKV 166
           RED+ P++ E+L+K+I+ +EEELK K+++IKK  RL +  +K +K+Q++KH +ELEKV
Sbjct: 99  REDRAPSRAESLKKDIAVMEEELKTKDELIKKHMRLFQESQKLVKEQIEKHRDELEKV 156

BLAST of Lsi03G010350 vs. ExPASy TrEMBL
Match: A0A1S3BZ89 (mediator of RNA polymerase II transcription subunit 28 OS=Cucumis melo OX=3656 GN=LOC103494796 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 3.0e-66
Identity = 136/141 (96.45%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 25  MAERQPLDQQHPLDPQSQSSQPPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 84
           MAERQ LDQQH LDPQSQSSQ PREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV
Sbjct: 1   MAERQSLDQQHSLDPQSQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 60

Query: 85  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKNDIIKKQERLI 144
           DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELK+KNDIIKKQERLI
Sbjct: 61  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKVKNDIIKKQERLI 120

Query: 145 EGWKKDLKDQLDKHNNELEKV 166
           EGWKKDLK+QLDKHNNELEKV
Sbjct: 121 EGWKKDLKEQLDKHNNELEKV 141

BLAST of Lsi03G010350 vs. ExPASy TrEMBL
Match: A0A5A7TRZ8 (Mediator of RNA polymerase II transcription subunit 28 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00780 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 3.0e-66
Identity = 136/141 (96.45%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 25  MAERQPLDQQHPLDPQSQSSQPPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 84
           MAERQ LDQQH LDPQSQSSQ PREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV
Sbjct: 1   MAERQSLDQQHSLDPQSQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 60

Query: 85  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKNDIIKKQERLI 144
           DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELK+KNDIIKKQERLI
Sbjct: 61  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKVKNDIIKKQERLI 120

Query: 145 EGWKKDLKDQLDKHNNELEKV 166
           EGWKKDLK+QLDKHNNELEKV
Sbjct: 121 EGWKKDLKEQLDKHNNELEKV 141

BLAST of Lsi03G010350 vs. ExPASy TrEMBL
Match: A0A0A0KT19 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G00990 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.7e-66
Identity = 134/141 (95.04%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 25  MAERQPLDQQHPLDPQSQSSQPPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 84
           MAERQP+DQQH LDPQ QSSQ PREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV
Sbjct: 1   MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 60

Query: 85  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKNDIIKKQERLI 144
           DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELK+KN+IIKKQE+LI
Sbjct: 61  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKVKNEIIKKQEKLI 120

Query: 145 EGWKKDLKDQLDKHNNELEKV 166
           EGWKKDLKDQLDKHNNELEKV
Sbjct: 121 EGWKKDLKDQLDKHNNELEKV 141

BLAST of Lsi03G010350 vs. ExPASy TrEMBL
Match: A0A6J1JAT8 (mediator of RNA polymerase II transcription subunit 28 OS=Cucurbita maxima OX=3661 GN=LOC111482804 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 4.4e-65
Identity = 132/141 (93.62%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 25  MAERQPLDQQHPLDPQSQSSQPPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 84
           MAERQPLDQQ+ +DPQ QSSQ PREDM++YVMALEAALLPCLPARELQAIDRSPHPSHQ+
Sbjct: 1   MAERQPLDQQNQIDPQLQSSQSPREDMVSYVMALEAALLPCLPARELQAIDRSPHPSHQI 60

Query: 85  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKNDIIKKQERLI 144
           DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLK+DIIKKQERLI
Sbjct: 61  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKDDIIKKQERLI 120

Query: 145 EGWKKDLKDQLDKHNNELEKV 166
           EGWKKDLKDQLDKHNNELEKV
Sbjct: 121 EGWKKDLKDQLDKHNNELEKV 141

BLAST of Lsi03G010350 vs. ExPASy TrEMBL
Match: A0A6J1E897 (mediator of RNA polymerase II transcription subunit 28 OS=Cucurbita moschata OX=3662 GN=LOC111430779 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 4.4e-65
Identity = 132/141 (93.62%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 25  MAERQPLDQQHPLDPQSQSSQPPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 84
           MAERQPLDQQ+ +DPQ QSSQ PREDM++YVMALEAALLPCLPARELQAIDRSPHPSHQ+
Sbjct: 1   MAERQPLDQQNQIDPQLQSSQSPREDMVSYVMALEAALLPCLPARELQAIDRSPHPSHQI 60

Query: 85  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKNDIIKKQERLI 144
           DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLK+DIIKKQERLI
Sbjct: 61  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKDDIIKKQERLI 120

Query: 145 EGWKKDLKDQLDKHNNELEKV 166
           EGWKKDLKDQLDKHNNELEKV
Sbjct: 121 EGWKKDLKDQLDKHNNELEKV 141

BLAST of Lsi03G010350 vs. NCBI nr
Match: XP_038904970.1 (mediator of RNA polymerase II transcription subunit 28 [Benincasa hispida] >XP_038904972.1 mediator of RNA polymerase II transcription subunit 28 [Benincasa hispida] >XP_038904973.1 mediator of RNA polymerase II transcription subunit 28 [Benincasa hispida] >XP_038904974.1 mediator of RNA polymerase II transcription subunit 28 [Benincasa hispida])

HSP 1 Score: 264.2 bits (674), Expect = 7.4e-67
Identity = 137/141 (97.16%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 25  MAERQPLDQQHPLDPQSQSSQPPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 84
           MAERQPLDQQHPLDPQ QSSQ PREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV
Sbjct: 1   MAERQPLDQQHPLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 60

Query: 85  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKNDIIKKQERLI 144
           DVERHARDFMEAAKKLQLYFIGLQREDQP KVETLRKEISAIEEELKLKN+IIKKQERLI
Sbjct: 61  DVERHARDFMEAAKKLQLYFIGLQREDQPNKVETLRKEISAIEEELKLKNEIIKKQERLI 120

Query: 145 EGWKKDLKDQLDKHNNELEKV 166
           EGWKKDLKDQLDKHNNELEKV
Sbjct: 121 EGWKKDLKDQLDKHNNELEKV 141

BLAST of Lsi03G010350 vs. NCBI nr
Match: XP_008454378.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 28 [Cucumis melo] >XP_008454380.1 PREDICTED: mediator of RNA polymerase II transcription subunit 28 [Cucumis melo] >KAA0044351.1 mediator of RNA polymerase II transcription subunit 28 [Cucumis melo var. makuwa] >TYK29480.1 mediator of RNA polymerase II transcription subunit 28 [Cucumis melo var. makuwa])

HSP 1 Score: 261.2 bits (666), Expect = 6.2e-66
Identity = 136/141 (96.45%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 25  MAERQPLDQQHPLDPQSQSSQPPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 84
           MAERQ LDQQH LDPQSQSSQ PREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV
Sbjct: 1   MAERQSLDQQHSLDPQSQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 60

Query: 85  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKNDIIKKQERLI 144
           DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELK+KNDIIKKQERLI
Sbjct: 61  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKVKNDIIKKQERLI 120

Query: 145 EGWKKDLKDQLDKHNNELEKV 166
           EGWKKDLK+QLDKHNNELEKV
Sbjct: 121 EGWKKDLKEQLDKHNNELEKV 141

BLAST of Lsi03G010350 vs. NCBI nr
Match: XP_004152267.1 (mediator of RNA polymerase II transcription subunit 28 [Cucumis sativus] >KGN52780.1 hypothetical protein Csa_014841 [Cucumis sativus])

HSP 1 Score: 260.0 bits (663), Expect = 1.4e-65
Identity = 134/141 (95.04%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 25  MAERQPLDQQHPLDPQSQSSQPPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 84
           MAERQP+DQQH LDPQ QSSQ PREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV
Sbjct: 1   MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 60

Query: 85  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKNDIIKKQERLI 144
           DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELK+KN+IIKKQE+LI
Sbjct: 61  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKVKNEIIKKQEKLI 120

Query: 145 EGWKKDLKDQLDKHNNELEKV 166
           EGWKKDLKDQLDKHNNELEKV
Sbjct: 121 EGWKKDLKDQLDKHNNELEKV 141

BLAST of Lsi03G010350 vs. NCBI nr
Match: XP_022922953.1 (mediator of RNA polymerase II transcription subunit 28 [Cucurbita moschata] >XP_022922954.1 mediator of RNA polymerase II transcription subunit 28 [Cucurbita moschata] >XP_022984538.1 mediator of RNA polymerase II transcription subunit 28 [Cucurbita maxima] >XP_022984539.1 mediator of RNA polymerase II transcription subunit 28 [Cucurbita maxima] >XP_023552182.1 mediator of RNA polymerase II transcription subunit 28 [Cucurbita pepo subsp. pepo] >XP_023552183.1 mediator of RNA polymerase II transcription subunit 28 [Cucurbita pepo subsp. pepo] >KAG6576925.1 Mediator of RNA polymerase II transcription subunit 28, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014951.1 Mediator of RNA polymerase II transcription subunit 28 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 257.3 bits (656), Expect = 9.0e-65
Identity = 132/141 (93.62%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 25  MAERQPLDQQHPLDPQSQSSQPPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 84
           MAERQPLDQQ+ +DPQ QSSQ PREDM++YVMALEAALLPCLPARELQAIDRSPHPSHQ+
Sbjct: 1   MAERQPLDQQNQIDPQLQSSQSPREDMVSYVMALEAALLPCLPARELQAIDRSPHPSHQI 60

Query: 85  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKNDIIKKQERLI 144
           DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLK+DIIKKQERLI
Sbjct: 61  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKDDIIKKQERLI 120

Query: 145 EGWKKDLKDQLDKHNNELEKV 166
           EGWKKDLKDQLDKHNNELEKV
Sbjct: 121 EGWKKDLKDQLDKHNNELEKV 141

BLAST of Lsi03G010350 vs. NCBI nr
Match: XP_022149222.1 (mediator of RNA polymerase II transcription subunit 28 [Momordica charantia])

HSP 1 Score: 253.1 bits (645), Expect = 1.7e-63
Identity = 131/141 (92.91%), Postives = 134/141 (95.04%), Query Frame = 0

Query: 25  MAERQPLDQQHPLDPQSQSSQPPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQV 84
           MA+RQP+DQQH LDPQ QS Q PREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ+
Sbjct: 1   MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQI 60

Query: 85  DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKLKNDIIKKQERLI 144
           DVERHARDFMEAAKKLQLYFIGLQREDQP KVETLRKEISAIEEELKLKNDIIKK ERLI
Sbjct: 61  DVERHARDFMEAAKKLQLYFIGLQREDQPIKVETLRKEISAIEEELKLKNDIIKKHERLI 120

Query: 145 EGWKKDLKDQLDKHNNELEKV 166
           EGWKKDLKDQL KHNNELEKV
Sbjct: 121 EGWKKDLKDQLAKHNNELEKV 141

BLAST of Lsi03G010350 vs. TAIR 10
Match: AT3G52860.1 (unknown protein; Has 86 Blast hits to 86 proteins in 36 species: Archae - 0; Bacteria - 2; Metazoa - 41; Fungi - 13; Plants - 26; Viruses - 0; Other Eukaryotes - 4 (source: NCBI BLink). )

HSP 1 Score: 160.2 bits (404), Expect = 1.4e-39
Identity = 82/118 (69.49%), Postives = 105/118 (88.98%), Query Frame = 0

Query: 49  EDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQ 108
           ED++A V ALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYF+GL+
Sbjct: 39  EDIMACVTALEAALLPCLPARELQAIDRSPHPSHQIDVERHARDFMEAAKKLQLYFMGLK 98

Query: 109 REDQ-PTKVETLRKEISAIEEELKLKNDIIKKQERLIEGWKKDLKDQLDKHNNELEKV 166
           RED+ P++ E+L+K+I+ +EEELK K+++IKK  RL +  +K +K+Q++KH +ELEKV
Sbjct: 99  REDRAPSRAESLKKDIAVMEEELKTKDELIKKHMRLFQESQKLVKEQIEKHRDELEKV 156

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFA52.0e-3869.49Mediator of RNA polymerase II transcription subunit 28 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A1S3BZ893.0e-6696.45mediator of RNA polymerase II transcription subunit 28 OS=Cucumis melo OX=3656 G... [more]
A0A5A7TRZ83.0e-6696.45Mediator of RNA polymerase II transcription subunit 28 OS=Cucumis melo var. maku... [more]
A0A0A0KT196.7e-6695.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G00990 PE=4 SV=1[more]
A0A6J1JAT84.4e-6593.62mediator of RNA polymerase II transcription subunit 28 OS=Cucurbita maxima OX=36... [more]
A0A6J1E8974.4e-6593.62mediator of RNA polymerase II transcription subunit 28 OS=Cucurbita moschata OX=... [more]
Match NameE-valueIdentityDescription
XP_038904970.17.4e-6797.16mediator of RNA polymerase II transcription subunit 28 [Benincasa hispida] >XP_0... [more]
XP_008454378.16.2e-6696.45PREDICTED: mediator of RNA polymerase II transcription subunit 28 [Cucumis melo]... [more]
XP_004152267.11.4e-6595.04mediator of RNA polymerase II transcription subunit 28 [Cucumis sativus] >KGN527... [more]
XP_022922953.19.0e-6593.62mediator of RNA polymerase II transcription subunit 28 [Cucurbita moschata] >XP_... [more]
XP_022149222.11.7e-6392.91mediator of RNA polymerase II transcription subunit 28 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G52860.11.4e-3969.49unknown protein; Has 86 Blast hits to 86 proteins in 36 species: Archae - 0; Bac... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 116..157
NoneNo IPR availablePFAMPF11594Med28coord: 54..149
e-value: 3.6E-18
score: 65.8
NoneNo IPR availablePANTHERPTHR39117:SF1MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 28coord: 25..165
IPR034456Mediator of RNA polymerase II transcription subunit 28PANTHERPTHR39117MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 28coord: 25..165

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G010350.1Lsi03G010350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0016592 mediator complex