Lsi03G010230 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G010230
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Trypsin inhibitor 1-like
Location	chr03: 19734897 .. 19735184 (-)
RNA-Seq Expression	Lsi03G010230
Synteny	Lsi03G010230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAGCAAGAAAATTGTTGTTGTGGCAATAATGGCAATGATAATGCTGGTGGCTATGTCGTTGGCTGCATTTGCCGTTGATGACGACTCGGTGGATCTCGTCTCTGACGGTCGAGCTCAAACTGGTCAAGACATCAAGAATGGATGCCCAAGAAAGATAATGATCATGAAAGGAAAAGTGTTTAAGGAGGAAGAAAGAAGATGTCCCAGAATCTACATGGAGTGCAAGCATGACTCAGATTGCTTAGCTGATTGTGTTTGCCTAGAGCATGGAATTTGTGGCTGA

mRNA sequence

ATGGAGAGCAAGAAAATTGTTGTTGTGGCAATAATGGCAATGATAATGCTGGTGGCTATGTCGTTGGCTGCATTTGCCGTTGATGACGACTCGGTGGATCTCGTCTCTGACGGTCGAGCTCAAACTGGTCAAGACATCAAGAATGGATGCCCAAGAAAGATAATGATCATGAAAGGAAAAGTGTTTAAGGAGGAAGAAAGAAGATGTCCCAGAATCTACATGGAGTGCAAGCATGACTCAGATTGCTTAGCTGATTGTGTTTGCCTAGAGCATGGAATTTGTGGCTGA

Coding sequence (CDS)

ATGGAGAGCAAGAAAATTGTTGTTGTGGCAATAATGGCAATGATAATGCTGGTGGCTATGTCGTTGGCTGCATTTGCCGTTGATGACGACTCGGTGGATCTCGTCTCTGACGGTCGAGCTCAAACTGGTCAAGACATCAAGAATGGATGCCCAAGAAAGATAATGATCATGAAAGGAAAAGTGTTTAAGGAGGAAGAAAGAAGATGTCCCAGAATCTACATGGAGTGCAAGCATGACTCAGATTGCTTAGCTGATTGTGTTTGCCTAGAGCATGGAATTTGTGGCTGA

Protein sequence

MESKKIVVVAIMAMIMLVAMSLAAFAVDDDSVDLVSDGRAQTGQDIKNGCPRKIMIMKGKVFKEEERRCPRIYMECKHDSDCLADCVCLEHGICG

Homology

[Close]

BLAST of Lsi03G010230 vs. ExPASy Swiss-Prot
Match: P26771 (Trypsin inhibitor 1 OS=Lagenaria siceraria OX=3668 PE=1 SV=2)

HSP 1 Score: 72.8 bits (177), Expect = 2.4e-12
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 66 ERRCPRIYMECKHDSDCLADCVCLEHGICG 96
          +RRCPRIYMECKHDSDCLADCVCLEHGICG
Sbjct: 1  QRRCPRIYMECKHDSDCLADCVCLEHGICG 30

[Close]

BLAST of Lsi03G010230 vs. ExPASy Swiss-Prot
Match: P11969 (Trypsin inhibitor 1 OS=Citrullus lanatus OX=3654 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-10
Identity = 26/29 (89.66%), Postives = 28/29 (96.55%), Query Frame = 0


Query: 67 RRCPRIYMECKHDSDCLADCVCLEHGICG 96
          RRCPRIYMECK D+DCLADCVCL+HGICG
Sbjct: 2  RRCPRIYMECKRDADCLADCVCLQHGICG 30

[Close]

BLAST of Lsi03G010230 vs. ExPASy Swiss-Prot
Match: P10293 (Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 7.1e-09
Identity = 24/31 (77.42%), Postives = 27/31 (87.10%), Query Frame = 0


Query: 65 EERRCPRIYMECKHDSDCLADCVCLEHGICG 96
          EER CP+I MECK DSDCLA+C+CLEHG CG
Sbjct: 2  EERVCPKILMECKKDSDCLAECICLEHGYCG 32

[Close]

BLAST of Lsi03G010230 vs. ExPASy Swiss-Prot
Match: P07853 (Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 7.1e-09
Identity = 25/31 (80.65%), Postives = 27/31 (87.10%), Query Frame = 0


Query: 65 EERRCPRIYMECKHDSDCLADCVCLEHGICG 96
          EER CPRI M+CK DSDCLA+CVCLEHG CG
Sbjct: 2  EERVCPRILMKCKKDSDCLAECVCLEHGYCG 32

[Close]

BLAST of Lsi03G010230 vs. ExPASy Swiss-Prot
Match: P01074 (Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 3.5e-08
Identity = 24/29 (82.76%), Postives = 25/29 (86.21%), Query Frame = 0


Query: 67 RRCPRIYMECKHDSDCLADCVCLEHGICG 96
          R CPRI MECK DSDCLA+CVCLEHG CG
Sbjct: 1  RVCPRILMECKKDSDCLAECVCLEHGYCG 29

[Close]

BLAST of Lsi03G010230 vs. ExPASy TrEMBL
Match: A0A0A0KV23 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.0e-22
Identity = 67/94 (71.28%), Postives = 76/94 (80.85%), Query Frame = 0


Query: 1  MESKKIVVVAIMAMIML-VAM-SLAAFAVDD-DSVDLVSDGRAQTGQDIKNGCPRKIMIM 60
          MESKKIV+VAI+A I++ VAM S A+F VDD D + LVSDGRAQ  QD+ NGCPRK+MI 
Sbjct: 1  MESKKIVMVAIVATIIVQVAMSSSASFVVDDKDWIKLVSDGRAQADQDMNNGCPRKMMI- 60

Query: 61 KGKVFKEEERRCPRIYMECKHDSDCLADCVCLEH 92
          KG VFKEEE  CPRI M+CKHDSDCL  CVCLEH
Sbjct: 61 KGGVFKEEEMMCPRILMKCKHDSDCLPGCVCLEH 93

[Close]

BLAST of Lsi03G010230 vs. ExPASy TrEMBL
Match: A0A5D3E148 (Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00640 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.7e-21
Identity = 64/98 (65.31%), Postives = 76/98 (77.55%), Query Frame = 0


Query: 1  MESKKIVVVAIMAMIMLVA--MSLAAFAVDD-DSVDLVSDGRAQTGQDIKNGCPRKIMIM 60
          M+SKKIVVVAI+ MI+++A   S A+F VDD DS +LVSDGR     D+ NGCPRKIMI 
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGR-----DMNNGCPRKIMI- 60

Query: 61 KGKVFKEEERRCPRIYMECKHDSDCLADCVCLEHGICG 96
          KG VFKEE+R CP+I M+CK DSDCL DCVCL+ G CG
Sbjct: 61 KGGVFKEEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

[Close]

BLAST of Lsi03G010230 vs. ExPASy TrEMBL
Match: A0A6J1E4H0 (probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC111430533 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 5.0e-13
Identity = 48/87 (55.17%), Postives = 62/87 (71.26%), Query Frame = 0


Query: 1  MESKKIVVVAIMAMIMLVAMSLAAFAVDDDSVDLVSDGRAQTGQDIKNGCPRKIMIMKGK 60
          M S KIV VA++A++++  +S AA+A+DDDS+DLV DGR     DI +  PRK  IMK  
Sbjct: 1  MGSMKIVAVALIAILLVATLSSAAYAIDDDSMDLVFDGR-----DINHRVPRK--IMKWG 60

Query: 61 VFKEEERRCPRIYMECKHDSDCLADCV 88
          V   EER CP+I MECK DSDCLA+C+
Sbjct: 61 VLNHEERVCPKILMECKKDSDCLAECL 80

[Close]

BLAST of Lsi03G010230 vs. ExPASy TrEMBL
Match: A0A0A7HIA9 (TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.5e-09
Identity = 38/82 (46.34%), Postives = 50/82 (60.98%), Query Frame = 0


Query: 17 LVAMSLAAFAVDD---DSVDLVSDGRAQTGQDIKNGCPRKIMIMKGKVFKEEERRCPRIY 76
          LVAM L A + D    D++ L+S+GRAQTGQDI +          G V+ EE+R CP+I 
Sbjct: 4  LVAMMLVATSADSNGGDTIHLISNGRAQTGQDINS----------GGVYSEEQRACPKIL 63

Query: 77 MECKHDSDCLADCVCLEHGICG 96
            C+ DSDC   CVC ++G CG
Sbjct: 64 KRCRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of Lsi03G010230 vs. ExPASy TrEMBL
Match: A0A0A7HG47 (TIPRE1 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE1 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.0e-09
Identity = 38/82 (46.34%), Postives = 50/82 (60.98%), Query Frame = 0


Query: 17 LVAMSLAAFAVD---DDSVDLVSDGRAQTGQDIKNGCPRKIMIMKGKVFKEEERRCPRIY 76
          LVAM L A + D    D++ L+S+GRAQTGQDI +          G V+ EE+R CP+I 
Sbjct: 4  LVAMMLVATSADFNGGDTIHLISNGRAQTGQDINS----------GGVYSEEQRACPKIL 63

Query: 77 MECKHDSDCLADCVCLEHGICG 96
            C+ DSDC   CVC ++G CG
Sbjct: 64 KRCRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of Lsi03G010230 vs. NCBI nr
Match: KAA0044339.1 (trypsin inhibitor 1-like [Cucumis melo var. makuwa] >TYK29468.1 trypsin inhibitor 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 111.7 bits (278), Expect = 3.5e-21
Identity = 64/98 (65.31%), Postives = 76/98 (77.55%), Query Frame = 0


Query: 1  MESKKIVVVAIMAMIMLVA--MSLAAFAVDD-DSVDLVSDGRAQTGQDIKNGCPRKIMIM 60
          M+SKKIVVVAI+ MI+++A   S A+F VDD DS +LVSDGR     D+ NGCPRKIMI 
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGR-----DMNNGCPRKIMI- 60

Query: 61 KGKVFKEEERRCPRIYMECKHDSDCLADCVCLEHGICG 96
          KG VFKEE+R CP+I M+CK DSDCL DCVCL+ G CG
Sbjct: 61 KGGVFKEEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

[Close]

BLAST of Lsi03G010230 vs. NCBI nr
Match: KAG6576919.1 (hypothetical protein SDJN03_24493, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 104.8 bits (260), Expect = 4.3e-19
Identity = 56/95 (58.95%), Postives = 70/95 (73.68%), Query Frame = 0


Query: 1   MESKKIVVVAIMAMIMLVAMSLAAFAVDDDSVDLVSDGRAQTGQDIKNGCPRKIMIMKGK 60
           M S KIV VA++A++++  +S AA+A+DDDS+DLV DGR     DI +G PRK  IMK  
Sbjct: 54  MGSMKIVAVALIAILLVATLSSAAYAIDDDSMDLVFDGR-----DINHGVPRK--IMKWG 113

Query: 61  VFKEEERRCPRIYMECKHDSDCLADCVCLEHGICG 96
           V   EER CP+I MECK DSDCLA+C+CLEHG CG
Sbjct: 114 VLNHEERVCPKILMECKKDSDCLAECICLEHGYCG 141

[Close]

BLAST of Lsi03G010230 vs. NCBI nr
Match: KAG7014944.1 (hypothetical protein SDJN02_22575, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 104.8 bits (260), Expect = 4.3e-19
Identity = 56/95 (58.95%), Postives = 70/95 (73.68%), Query Frame = 0


Query: 1   MESKKIVVVAIMAMIMLVAMSLAAFAVDDDSVDLVSDGRAQTGQDIKNGCPRKIMIMKGK 60
           M S KIV VA++A++++  +S AA+A+DDDS+DLV DGR     DI +G PRK  IMK  
Sbjct: 54  MGSMKIVAVALIAILLVATLSSAAYAIDDDSMDLVFDGR-----DINHGVPRK--IMKWG 113

Query: 61  VFKEEERRCPRIYMECKHDSDCLADCVCLEHGICG 96
           V   EER CP+I MECK DSDCLA+C+CLEHG CG
Sbjct: 114 VLNHEERVCPKILMECKKDSDCLAECICLEHGYCG 141

[Close]

BLAST of Lsi03G010230 vs. NCBI nr
Match: XP_022922566.1 (probable polygalacturonase At3g15720 [Cucurbita moschata])

HSP 1 Score: 83.6 bits (205), Expect = 1.0e-12
Identity = 48/87 (55.17%), Postives = 62/87 (71.26%), Query Frame = 0


Query: 1  MESKKIVVVAIMAMIMLVAMSLAAFAVDDDSVDLVSDGRAQTGQDIKNGCPRKIMIMKGK 60
          M S KIV VA++A++++  +S AA+A+DDDS+DLV DGR     DI +  PRK  IMK  
Sbjct: 1  MGSMKIVAVALIAILLVATLSSAAYAIDDDSMDLVFDGR-----DINHRVPRK--IMKWG 60

Query: 61 VFKEEERRCPRIYMECKHDSDCLADCV 88
          V   EER CP+I MECK DSDCLA+C+
Sbjct: 61 VLNHEERVCPKILMECKKDSDCLAECL 80

[Close]

BLAST of Lsi03G010230 vs. NCBI nr
Match: XP_023552846.1 (probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 80.9 bits (198), Expect = 6.6e-12
Identity = 56/99 (56.57%), Postives = 65/99 (65.66%), Query Frame = 0


Query: 1  MESKKIVVVAIMAMIMLVAMSLAAFAVDDDSVDLVSDGRAQTGQDIKNGCPRKIMIMKGK 60
          M S K V VA++AM MLVA S AA+A+DDDS+DLV DGR     DI +G PRK  IMK  
Sbjct: 1  MGSMKNVAVALIAM-MLVATSSAAYAIDDDSMDLVFDGR-----DINHGVPRK--IMKWG 60

Query: 61 VFKEEERRCPRIYMECKHDSDCLA----DCVCLEHGICG 96
          V   EER CP+I MECK DSDCLA    D   L++G  G
Sbjct: 61 VLNHEERVCPKILMECKKDSDCLAESSSDFNVLDYGAVG 91

The following BLAST results are available for this feature:

BLAST of Lsi03G010230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P26771	2.4e-12	96.67	Trypsin inhibitor 1 OS=Lagenaria siceraria OX=3668 PE=1 SV=2	[more]
P11969	1.7e-10	89.66	Trypsin inhibitor 1 OS=Citrullus lanatus OX=3654 PE=1 SV=1	[more]
P10293	7.1e-09	77.42	Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]
P07853	7.1e-09	80.65	Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2	[more]
P01074	3.5e-08	82.76	Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]

BLAST of Lsi03G010230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KV23	2.0e-22	71.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1	[more]
A0A5D3E148	1.7e-21	65.31	Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A6J1E4H0	5.0e-13	55.17	probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC1114305...	[more]
A0A0A7HIA9	1.5e-09	46.34	TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1	[more]
A0A0A7HG47	2.0e-09	46.34	TIPRE1 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE1 PE=3 SV=1	[more]

BLAST of Lsi03G010230 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0044339.1	3.5e-21	65.31	trypsin inhibitor 1-like [Cucumis melo var. makuwa] >TYK29468.1 trypsin inhibito...	[more]
KAG6576919.1	4.3e-19	58.95	hypothetical protein SDJN03_24493, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014944.1	4.3e-19	58.95	hypothetical protein SDJN02_22575, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022922566.1	1.0e-12	55.17	probable polygalacturonase At3g15720 [Cucurbita moschata]	[more]
XP_023552846.1	6.6e-12	56.57	probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi03G010230 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PRINTS	PR00293	SQUASHINHBTR	coord: 79..88 score: 80.5 coord: 69..78 score: 73.0
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 67..95 e-value: 8.6E-18 score: 64.1
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 69..88
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 69..95 e-value: 8.47412E-12 score: 52.7651
None	No IPR available	SMART	SM00286	PTI_1	coord: 67..95 e-value: 1.2E-11 score: 54.7
None	No IPR available	GENE3D	4.10.75.20		coord: 59..95 e-value: 9.8E-13 score: 49.7
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 64..95

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G010230.1	Lsi03G010230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
cellular_component	GO:0016020	membrane
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity