Lsi03G010160 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G010160
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAdenylyl-sulfate kinase
Locationchr03: 19567621 .. 19570838 (-)
RNA-Seq ExpressionLsi03G010160
SyntenyLsi03G010160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCGCTTACATTTATTTATTGCGTTTTCGTCGGACAGTAAACGACGTCGTTCTCTTCTTCTTCTCCGGACGGCTCGCTGTTCCGCAGGCTCCGGCTCCGCATTCTTCTTCTACGGTTCATAAATTAAAGGGAAGAAGAAGAATCTAAATCAAAGGTTCAGAACGAGGGGTCATCGCCAAAAGGGCTCGTGATTCTTGTTTTTTTTTCCACCGGTCATTAATTATTATGGCGGACAAGAACAGGAGAGCCTCTCCGCCATTTGACTAATTATGGCGTCCCATTTCCCTTTGATTTCATCACTCGGTTTCTTTTTTAGTTTTTCTATTTATTTTTCAGTCAATTATGTTTCCCTTTTGAGATGTGTGTAAGATATGGTTGCGATTGGGGGAATTTCGTTGCCGGGTTTTGTGCCGCCGTCGCTCCACCACCAATCCTTTTCGCCATCGGCGATTGGGTTCGCGAGGTTTCCCGATAGGATTAGCTTCAGATCGTTGGTGGTTTTGCGGGATGGGGTTGAGAAGAAGGGTTCGAGGGTGGTGATTGTTAACGGGAAGGTCGATGGTTTGGGTAAGAGTGAATGCGAGACTGAGTTTGATACTACCTTGGGCAATGGACATGCTGGGAACTCGGGTATTGCTTCTTTTGATGTCTTTCGATGTAATCTTGAATCAAGGACTTTGATTTTGGGTGAATCAGAATATCTGTTATTTTTTGGTTCTTGAGATTTTGAATCATCTCATTGAAATTACTGGTGTTTTGGTTATCTTGTTTGATTCATTAGATAGTGGAAATAAGATAATTGGATGAGCAAGAACCTTATTGTTTGTATTTATGTAGATATTAGTTATGTTGTCATTGACATTTTCAGTATTTATCTACACATTTACAATTTGATGTTGAACTCTTTGATTTATAGGAATGCTGATGTAATTGCAGAATGTTTGAATTGTTTCTATGTTGGCATAAGCTTATATTGTGGTGTCCTATTTTTCTCTTCATGGTGTGAGTTTCTCTTCTGTAGTTTAAGCTGTATAGATTACTAAGATGCTTTTCAATCAGGAAAAAATCATGGAGTGTTATCCACAGTAGGAAATTCTACTAATATCAAGTGGCATGAATGTTCAGTTGGAAAGGTTGAAAAACAGAGCCTTCTCAAACAGAAAGGATGTGTCATATGGATCACAGGTCTCAGTGGCTCAGGTTTGTTATTTAACCTTTTCCTTTTGCTTTTACAGCATAATGGTTATTATCTTTTTATGTCATATTCATGGTTTAGAGAATCTATAGTTTAGATTGTCTTCAAATGAACCTATGGTTTGGATAACAGACCAAACCCAACCATGAAATATTTGAGTTTGATTTTTCAGGTCATTTTTTAAAAGATTTTGTGGAAAGGATAGCAAAAAAATTGGCCTGAAATAGTTAAGTTGGGTTTGGGACTTTTTGGACCTTACCCAATTCTTCTAAATATTCTTAGCTAACTTGCAGGGAAGAGCACTGTGGCCTGTGCCTTGAGTCAAAGCTTATACCAAATGGGAAAGTTGGCTTATATTCTTGATGGGGACAATGTCAGGCATGGCTTGAATCGTGACCTTGGTTTTAAAGCAGAAGATCGTGCTGAGAATATAAGGAGGGTCGGTAAGTTCGGTTCATTTACAACACTTTGGATTGGGCTGTTCATGATTGCTAATTATACCTCTTCCATAAATAGGTGAGGTTGCAAAACTGTTTGCAGACGCTGGAGTTATTTGCATTGCTAGTTTGATATCCCCATATCGAAGGGACCGAGATGCCTGTCGTGCCATTTTGCCTGATGGATACTTTATTGAGGTATCCCTTCATTTTATGCATACTATAGATTTTGAGCAGGCTTTTTACCTTGCATCTGAAATATTTATGACAAAAATGTGCTAAAAATATATAGGTGTTCATGGATGTTCCTCTTGAAGTCTGTGAAGCAAGAGATACAAAAGGACTGTATAAGCTTGCACGGGCAGGGAAGATCAAAGGTCACTGCAACTTGTTAACATCTTCCTTAGATTTACTTGATGATCTTGTTAATTCTCACTTATTTTTTAATGTGATTTGAGTATTACTTTAATCTTATTTTGGCTCCTAACTTTCACAACCTAAGTTTTGACTCAAAGATATTGTCGAATGGGCTTAAGTTAACTATATATGCTCAGAATTATCATAATTTCTCTATCAACTGAACAAAGTCGTATTTTTGCAGGCTTTACTGGTATCGATGATCCGTATGAAGTACCATTGAATTGTGAGGTAATTCCAAATTCTGGAGGAAAATGAAAACATCTCTTTAAACATTCTTGCAAAAAATCAATGCTTCTACTACATCGAAACTCGAAAGTAAATGTTTTTGCAATATATACTAATGGTTATGAATAAAGTGGTTTGTGTTATGAATGAGACATTGTTTCTGAATCTGATTGGGATACCCTTTTGAACTTTTTTGGTTTCAGATAGTACTGAAACACAATGGAGGTTTCCCATGTGAGATGGCAGAGAAAGTACTGAGTTACTTAGAGCAGAAGGGCTTCCTGCAGGCTTAAACTCAAACTCTATGAGAAAAGGGGCTCTTAACAGCTGAAGTTTTGGTTGAAATTTTTATTCTACTTTAGAGCAAATCCACTTAACTTAAGCTTTGGCTTTGCTTATTTGTTTTGTGACATCTAATTTGCTTCTTCTTTGTCCACTTCTTTTTCTGGATACAGATAGGAATGAAGGTTTGGCTTAGAATGTGATCTGAAAAAGGTTGTATGTGAGGATACATCAATTCATTTTGCTATTTTTTTTTTTTTTTTGCCCCCTTTGATCTGTGTAATGTAATATAGAGTTGTTTGAAACAGGGTCGAATGTTTTATACAAAAAGTATATCAAATATTTTTGTTGAAACTAGAAGTCAATCTCAGTCTCTCTCTCTCTAGTATATATACAAAAGGAGTTTCAAATCTATCTTATATCGCGAAAGCGAGTATAATTCAATTGTTATTGATCTATACTCCTTTTTTTCGAAATTGGAGGTTCAAATCTTCATATTTTCATTTGTTATGCTACAAAAAAATCTCTCTTATTTGAATAAAAAGAAGCTTGATGGTGTAAGTGAGAGAATTTGTGTTTAGGTGTAGGTTAGTGAGTGTGAGGAGAGAAACCAATTAGAGATTAGTGGGGAGAGGAGTTAGTGGGTGATATCTATGTGT

mRNA sequence

CTCGCTTACATTTATTTATTGCGTTTTCGTCGGACAGTAAACGACGTCGTTCTCTTCTTCTTCTCCGGACGGCTCGCTGTTCCGCAGGCTCCGGCTCCGCATTCTTCTTCTACGGTTCATAAATTAAAGGGAAGAAGAAGAATCTAAATCAAAGGTTCAGAACGAGGGGTCATCGCCAAAAGGGCTCGTGATTCTTGTTTTTTTTTCCACCGGTCATTAATTATTATGGCGGACAAGAACAGGAGAGCCTCTCCGCCATTTGACTAATTATGGCGTCCCATTTCCCTTTGATTTCATCACTCGGTTTCTTTTTTAGTTTTTCTATTTATTTTTCAGTCAATTATGTTTCCCTTTTGAGATGTGTGTAAGATATGGTTGCGATTGGGGGAATTTCGTTGCCGGGTTTTGTGCCGCCGTCGCTCCACCACCAATCCTTTTCGCCATCGGCGATTGGGTTCGCGAGGTTTCCCGATAGGATTAGCTTCAGATCGTTGGTGGTTTTGCGGGATGGGGTTGAGAAGAAGGGTTCGAGGGTGGTGATTGTTAACGGGAAGGTCGATGGTTTGGGTAAGAGTGAATGCGAGACTGAGTTTGATACTACCTTGGGCAATGGACATGCTGGGAACTCGGGAAAAAATCATGGAGTGTTATCCACAGTAGGAAATTCTACTAATATCAAGTGGCATGAATGTTCAGTTGGAAAGGTTGAAAAACAGAGCCTTCTCAAACAGAAAGGATGTGTCATATGGATCACAGGTCTCAGTGGCTCAGGGAAGAGCACTGTGGCCTGTGCCTTGAGTCAAAGCTTATACCAAATGGGAAAGTTGGCTTATATTCTTGATGGGGACAATGTCAGGCATGGCTTGAATCGTGACCTTGGTTTTAAAGCAGAAGATCGTGCTGAGAATATAAGGAGGGTCGGTGAGGTTGCAAAACTGTTTGCAGACGCTGGAGTTATTTGCATTGCTAGTTTGATATCCCCATATCGAAGGGACCGAGATGCCTGTCGTGCCATTTTGCCTGATGGATACTTTATTGAGGTGTTCATGGATGTTCCTCTTGAAGTCTGTGAAGCAAGAGATACAAAAGGACTGTATAAGCTTGCACGGGCAGGGAAGATCAAAGGCTTTACTGGTATCGATGATCCGTATGAAGTACCATTGAATTGTGAGATAGTACTGAAACACAATGGAGGTTTCCCATGTGAGATGGCAGAGAAAGTACTGAGTTACTTAGAGCAGAAGGGCTTCCTGCAGGCTTAAACTCAAACTCTATGAGAAAAGGGGCTCTTAACAGCTGAAGTTTTGATAGGAATGAAGGTTTGGCTTAGAATGTGATCTGAAAAAGGTTGTATGTGAGGATACATCAATTCATTTTGCTATTTTTTTTTTTTTTTTGCCCCCTTTGATCTGTGTAATGTAATATAGAGTTGTTTGAAACAGGGTCGAATGTTTTATACAAAAAGTATATCAAATATTTTTGTTGAAACTAGAAGTCAATCTCAGTCTCTCTCTCTCTAGTATATATACAAAAGGAGTTTCAAATCTATCTTATATCGCGAAAGCGAGTATAATTCAATTGTTATTGATCTATACTCCTTTTTTTCGAAATTGGAGGTTCAAATCTTCATATTTTCATTTGTTATGCTACAAAAAAATCTCTCTTATTTGAATAAAAAGAAGCTTGATGGTGTAAGTGAGAGAATTTGTGTTTAGGTGTAGGTTAGTGAGTGTGAGGAGAGAAACCAATTAGAGATTAGTGGGGAGAGGAGTTAGTGGGTGATATCTATGTGT

Coding sequence (CDS)

ATGGTTGCGATTGGGGGAATTTCGTTGCCGGGTTTTGTGCCGCCGTCGCTCCACCACCAATCCTTTTCGCCATCGGCGATTGGGTTCGCGAGGTTTCCCGATAGGATTAGCTTCAGATCGTTGGTGGTTTTGCGGGATGGGGTTGAGAAGAAGGGTTCGAGGGTGGTGATTGTTAACGGGAAGGTCGATGGTTTGGGTAAGAGTGAATGCGAGACTGAGTTTGATACTACCTTGGGCAATGGACATGCTGGGAACTCGGGAAAAAATCATGGAGTGTTATCCACAGTAGGAAATTCTACTAATATCAAGTGGCATGAATGTTCAGTTGGAAAGGTTGAAAAACAGAGCCTTCTCAAACAGAAAGGATGTGTCATATGGATCACAGGTCTCAGTGGCTCAGGGAAGAGCACTGTGGCCTGTGCCTTGAGTCAAAGCTTATACCAAATGGGAAAGTTGGCTTATATTCTTGATGGGGACAATGTCAGGCATGGCTTGAATCGTGACCTTGGTTTTAAAGCAGAAGATCGTGCTGAGAATATAAGGAGGGTCGGTGAGGTTGCAAAACTGTTTGCAGACGCTGGAGTTATTTGCATTGCTAGTTTGATATCCCCATATCGAAGGGACCGAGATGCCTGTCGTGCCATTTTGCCTGATGGATACTTTATTGAGGTGTTCATGGATGTTCCTCTTGAAGTCTGTGAAGCAAGAGATACAAAAGGACTGTATAAGCTTGCACGGGCAGGGAAGATCAAAGGCTTTACTGGTATCGATGATCCGTATGAAGTACCATTGAATTGTGAGATAGTACTGAAACACAATGGAGGTTTCCCATGTGAGATGGCAGAGAAAGTACTGAGTTACTTAGAGCAGAAGGGCTTCCTGCAGGCTTAA

Protein sequence

MVAIGGISLPGFVPPSLHHQSFSPSAIGFARFPDRISFRSLVVLRDGVEKKGSRVVIVNGKVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLKQKGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGFPCEMAEKVLSYLEQKGFLQA
Homology
BLAST of Lsi03G010160 vs. ExPASy Swiss-Prot
Match: Q43295 (Adenylyl-sulfate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK1 PE=1 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 2.7e-99
Identity = 187/259 (72.20%), Postives = 208/259 (80.31%), Query Frame = 0

Query: 39  RSLVVLRDGVEKKGSRVVIVNGKVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGN 98
           RS+VV+R  V   GS+ +  N       K+    E  +   NGH   +G+  G LSTVGN
Sbjct: 30  RSVVVVRACVSMDGSQTLSHN-------KNGSIPEVKSI--NGH---TGQKQGPLSTVGN 89

Query: 99  STNIKWHECSVGKVEKQSLLKQKGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDG 158
           STNIKWHECSV KV++Q LL QKGCVIW+TGLSGSGKST+ACAL+Q LYQ GKL YILDG
Sbjct: 90  STNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLACALNQMLYQKGKLCYILDG 149

Query: 159 DNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPD 218
           DNVRHGLNRDL FKAEDRAENIRRVGEVAKLFADAG+ICIASLISPYR DRDACR++LP+
Sbjct: 150 DNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIASLISPYRTDRDACRSLLPE 209

Query: 219 GYFIEVFMDVPLEVCEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGG-FP 278
           G F+EVFMDVPL VCEARD KGLYKLARAGKIKGFTGIDDPYE PLNCEI L   GG  P
Sbjct: 210 GDFVEVFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDDPYEPPLNCEISLGREGGTSP 269

Query: 279 CEMAEKVLSYLEQKGFLQA 297
            EMAEKV+ YL+ KG+LQA
Sbjct: 270 IEMAEKVVGYLDNKGYLQA 276

BLAST of Lsi03G010160 vs. ExPASy Swiss-Prot
Match: O49204 (Adenylyl-sulfate kinase, chloroplastic OS=Catharanthus roseus OX=4058 GN=AKN PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 2.7e-91
Identity = 171/245 (69.80%), Postives = 198/245 (80.82%), Query Frame = 0

Query: 58  VNGKVDG---LGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKVEK 117
           VNGKVD    L  S+C +  D++L N + G  GK     +TVGNSTNI WH+C+V K E+
Sbjct: 69  VNGKVDNFRHLQPSDCNSNSDSSLNNCN-GFPGKKILQTTTVGNSTNILWHKCAVEKSER 128

Query: 118 QSLLKQKGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDGDNVRHGLNRDLGFKAE 177
           Q  L+Q+GCVIWITGLSGSGKST+ACALS+ L+  GKL YILDGDNVRHGLN DL FKAE
Sbjct: 129 QEPLQQRGCVIWITGLSGSGKSTLACALSRGLHAKGKLTYILDGDNVRHGLNSDLSFKAE 188

Query: 178 DRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCE 237
           DRAENIRR+GEVAKLFADAGVICIASLISPYR+  DACR++LP+G FIEVFMDVPL+VCE
Sbjct: 189 DRAENIRRIGEVAKLFADAGVICIASLISPYRKPPDACRSLLPEGDFIEVFMDVPLKVCE 248

Query: 238 ARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGF---PCEMAEKVLSYLEQK 297
           ARD KGLYKLARAGKIKGFTGIDDPYE PL  EIVL    G    PC++A+ V+SYLE+ 
Sbjct: 249 ARDPKGLYKLARAGKIKGFTGIDDPYEPPLKSEIVLHQKLGMCDSPCDLADIVISYLEEN 308

BLAST of Lsi03G010160 vs. ExPASy Swiss-Prot
Match: O49196 (Adenylyl-sulfate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK2 PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.1e-87
Identity = 153/213 (71.83%), Postives = 179/213 (84.04%), Query Frame = 0

Query: 87  GKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLKQKGCVIWITGLSGSGKSTVACALSQSL 146
           G+N+G       + NI WHE S+ + ++Q LL+QKGCV+WITGLSGSGKSTVACALS++L
Sbjct: 84  GRNNG----KEKAENIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALSKAL 143

Query: 147 YQMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYR 206
           ++ GKL Y LDGDNVRHGLNRDL FKAE R ENIRR+GEVAKLFAD GVICIASLISPYR
Sbjct: 144 FERGKLTYTLDGDNVRHGLNRDLTFKAEHRTENIRRIGEVAKLFADVGVICIASLISPYR 203

Query: 207 RDRDACRAILPDGYFIEVFMDVPLEVCEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNC 266
           RDRDACR++LPDG F+EVFMDVPL VCE+RD KGLYKLARAGKIKGFTGIDDPYE P+NC
Sbjct: 204 RDRDACRSLLPDGDFVEVFMDVPLHVCESRDPKGLYKLARAGKIKGFTGIDDPYEAPVNC 263

Query: 267 EIVLKHNGG----FPCEMAEKVLSYLEQKGFLQ 296
           E+VLKH G      P +MAE ++SYL+ KG+L+
Sbjct: 264 EVVLKHTGDDESCSPRQMAENIISYLQNKGYLE 292

BLAST of Lsi03G010160 vs. ExPASy Swiss-Prot
Match: Q9SRW7 (Adenylyl-sulfate kinase 3 OS=Arabidopsis thaliana OX=3702 GN=APK3 PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.1e-87
Identity = 155/206 (75.24%), Postives = 176/206 (85.44%), Query Frame = 0

Query: 93  LSTVGNSTNIKWHECSVGKVEKQSLLKQKGCVIWITGLSGSGKSTVACALSQSLYQMGKL 152
           +STVGNSTNI W E  +GK E+Q LL QKGCV+WITGLSGSGKST+AC+LS+ L   GKL
Sbjct: 1   MSTVGNSTNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKL 60

Query: 153 AYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDAC 212
           +YILDGDN+RHGLN+DLGFKAEDR ENIRRVGEVAKLFADAG+ICIASLISPYR+DRDAC
Sbjct: 61  SYILDGDNLRHGLNKDLGFKAEDRVENIRRVGEVAKLFADAGLICIASLISPYRKDRDAC 120

Query: 213 RAILPDGYFIEVFMDVPLEVCEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH 272
           R ++ +  FIEVFM++ L++CEARD KGLYKLARAGKIKGFTGIDDPYE PLNCEI LK 
Sbjct: 121 REMIQNSSFIEVFMNMSLQLCEARDPKGLYKLARAGKIKGFTGIDDPYESPLNCEIELKE 180

Query: 273 NGG---FPCEMAEKVLSYLEQKGFLQ 296
             G    P  MAE+V+SYLE KGFLQ
Sbjct: 181 KEGECPSPVAMAEEVISYLEDKGFLQ 206

BLAST of Lsi03G010160 vs. ExPASy Swiss-Prot
Match: Q84JF0 (Adenylyl-sulfate kinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK4 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.0e-81
Identity = 146/217 (67.28%), Postives = 173/217 (79.72%), Query Frame = 0

Query: 97  GNSTNIKWHECSVGKVEKQSLLKQKGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYIL 156
           G   NI WH+C V K ++Q L+KQKGCVIWITGLSGSGKS++ACALS++L+  GKL+YIL
Sbjct: 84  GKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSLACALSRALHNRGKLSYIL 143

Query: 157 DGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAIL 216
           DGDNVRHGLN DL F+A+DRAENIRRVGEVAKLFAD+G+ICIASLISPYR +R ACRA+L
Sbjct: 144 DGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICIASLISPYRIERAACRALL 203

Query: 217 PDGYFIEVFMDVPLEVCEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH---- 276
           P G FIEVFMDVPL VCEARD KGLYK ARAGKIKGFTG+DDPYE PL+CEIV+++    
Sbjct: 204 PQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSRDK 263

Query: 277 --------------NGGFPCEMAEKVLSYLEQKGFLQ 296
                         +    CEMA+ V+SYL+Q G+L+
Sbjct: 264 GLSSSSSSSSSPSSSSSSLCEMADIVVSYLDQNGYLK 300

BLAST of Lsi03G010160 vs. ExPASy TrEMBL
Match: A0A1S3BYH9 (Adenylyl-sulfate kinase OS=Cucumis melo OX=3656 GN=LOC103494812 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.7e-155
Identity = 277/296 (93.58%), Postives = 280/296 (94.59%), Query Frame = 0

Query: 1   MVAIGGISLPGFVPPSLHHQSFSPSAIGFARFPDRISFRSLVVLRDGVEKKGSRVVIVNG 60
           MV+IG ISLPG VPPSLHHQ   PSAIGFARFPDRISFRS VVLRD VEKKGSRVVIVNG
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  KVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLKQ 120
           KVDGLGKSECETEFDT LGNG AGNSGKNHGVLSTVGNSTNIKWHECSVGK EKQSLLKQ
Sbjct: 61  KVDGLGKSECETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIW TGLSGSGKSTVACALSQSL +MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDTKG 240
           RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARD KG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGFPCEMAEKVLSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH GG P EMAEKVL+YLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQA 296

BLAST of Lsi03G010160 vs. ExPASy TrEMBL
Match: A0A5A7TN80 (Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00500 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.7e-155
Identity = 277/296 (93.58%), Postives = 280/296 (94.59%), Query Frame = 0

Query: 1   MVAIGGISLPGFVPPSLHHQSFSPSAIGFARFPDRISFRSLVVLRDGVEKKGSRVVIVNG 60
           MV+IG ISLPG VPPSLHHQ   PSAIGFARFPDRISFRS VVLRD VEKKGSRVVIVNG
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  KVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLKQ 120
           KVDGLGKSECETEFDT LGNG AGNSGKNHGVLSTVGNSTNIKWHECSVGK EKQSLLKQ
Sbjct: 61  KVDGLGKSECETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIW TGLSGSGKSTVACALSQSL +MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDTKG 240
           RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARD KG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGFPCEMAEKVLSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH GG P EMAEKVL+YLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQA 296

BLAST of Lsi03G010160 vs. ExPASy TrEMBL
Match: A0A5D3DZZ6 (Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00510 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.1e-154
Identity = 276/295 (93.56%), Postives = 279/295 (94.58%), Query Frame = 0

Query: 1   MVAIGGISLPGFVPPSLHHQSFSPSAIGFARFPDRISFRSLVVLRDGVEKKGSRVVIVNG 60
           MV+IG ISLPG VPPSLHHQ   PSAIGFARFPDRISFRS VVLRD VEKKGSRVVIVNG
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  KVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLKQ 120
           KVDGLGKSECETEFDT LGNG AGNSGKNHGVLSTVGNSTNIKWHECSVGK EKQSLLKQ
Sbjct: 61  KVDGLGKSECETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIW TGLSGSGKSTVACALSQSL +MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDTKG 240
           RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARD KG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGFPCEMAEKVLSYLEQKGFLQ 296
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH GG P EMAEKVL+YLEQKGFLQ
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQ 295

BLAST of Lsi03G010160 vs. ExPASy TrEMBL
Match: A0A0A0KY24 (Adenylyl-sulfate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000720 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.6e-153
Identity = 272/296 (91.89%), Postives = 278/296 (93.92%), Query Frame = 0

Query: 1   MVAIGGISLPGFVPPSLHHQSFSPSAIGFARFPDRISFRSLVVLRDGVEKKGSRVVIVNG 60
           MV+IGGISLPG +PP LHHQS   SAIGFARFPDRISFRS VVLRD VEKKGSRVVI NG
Sbjct: 1   MVSIGGISLPGSIPPLLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIANG 60

Query: 61  KVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLKQ 120
           KVDGLGKSECET+FDT LGNG AGNSGKNHGVLSTVGNSTNIKWHECSVGK EKQSLLKQ
Sbjct: 61  KVDGLGKSECETDFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIW TGLSGSGKSTVACALSQSLY+MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDTKG 240
           RRVGEVAKLFADAGVICIASLISPYRR+RDACR ILP+GYFIEVFMDVPLEVCEARD KG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGFPCEMAEKVLSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVP NCEIVLKH GG P EMAEKVLSYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVLSYLEQKGFLQA 296

BLAST of Lsi03G010160 vs. ExPASy TrEMBL
Match: A0A6J1J8X7 (Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482821 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.8e-147
Identity = 259/297 (87.21%), Postives = 276/297 (92.93%), Query Frame = 0

Query: 1   MVAIGGISLPGFVPPSLHHQSFS-PSAIGFARFPDRISFRSLVVLRDGVEKKGSRVVIVN 60
           MV+ GGIS PG VPP L H+SFS PSA GFARF + ISF+S VV+ D  EKKGSR VIVN
Sbjct: 1   MVSTGGISFPGCVPPPLRHKSFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60

Query: 61  GKVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLK 120
           GKVDGLGKSECE + D TLGNGHAGNSGKN GVLSTVGNSTNIKWHECS+G VE+QSLL 
Sbjct: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGKNAGVLSTVGNSTNIKWHECSIGNVERQSLLN 120

Query: 121 QKGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDGDNVRHGLNRDLGFKAEDRAEN 180
           QKGCVIWITGLSGSGKS+VACAL++SLY+MGKLAYILDGDNVRHGLNRDLGFKAEDRAEN
Sbjct: 121 QKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAEN 180

Query: 181 IRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDTK 240
           IRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLE+CEARDTK
Sbjct: 181 IRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEICEARDTK 240

Query: 241 GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGFPCEMAEKVLSYLEQKGFLQA 297
           GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGG PC+MA+KV+SYLEQKGFL+A
Sbjct: 241 GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSYLEQKGFLKA 297

BLAST of Lsi03G010160 vs. NCBI nr
Match: XP_038903366.1 (adenylyl-sulfate kinase 3-like [Benincasa hispida])

HSP 1 Score: 578.2 bits (1489), Expect = 4.1e-161
Identity = 285/296 (96.28%), Postives = 288/296 (97.30%), Query Frame = 0

Query: 1   MVAIGGISLPGFVPPSLHHQSFSPSAIGFARFPDRISFRSLVVLRDGVEKKGSRVVIVNG 60
           MVAIGGISLPGF+PPSLHHQSFSPSAIGFARFPDRISFRS VV RDGVEKK SRVVIVNG
Sbjct: 1   MVAIGGISLPGFLPPSLHHQSFSPSAIGFARFPDRISFRSSVVWRDGVEKKDSRVVIVNG 60

Query: 61  KVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLKQ 120
           KVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGK EKQSLLKQ
Sbjct: 61  KVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKSTVACALSQSLY+MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDTKG 240
           RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARD KG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGFPCEMAEKVLSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVPLNCEI+LKHNGG P EMA KVLSYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEILLKHNGGSPSEMAGKVLSYLEQKGFLQA 296

BLAST of Lsi03G010160 vs. NCBI nr
Match: XP_008454396.1 (PREDICTED: adenylyl-sulfate kinase 3 [Cucumis melo] >XP_008454397.1 PREDICTED: adenylyl-sulfate kinase 3 [Cucumis melo] >KAA0044328.1 adenylyl-sulfate kinase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 557.4 bits (1435), Expect = 7.6e-155
Identity = 277/296 (93.58%), Postives = 280/296 (94.59%), Query Frame = 0

Query: 1   MVAIGGISLPGFVPPSLHHQSFSPSAIGFARFPDRISFRSLVVLRDGVEKKGSRVVIVNG 60
           MV+IG ISLPG VPPSLHHQ   PSAIGFARFPDRISFRS VVLRD VEKKGSRVVIVNG
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  KVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLKQ 120
           KVDGLGKSECETEFDT LGNG AGNSGKNHGVLSTVGNSTNIKWHECSVGK EKQSLLKQ
Sbjct: 61  KVDGLGKSECETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIW TGLSGSGKSTVACALSQSL +MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDTKG 240
           RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARD KG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGFPCEMAEKVLSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH GG P EMAEKVL+YLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQA 296

BLAST of Lsi03G010160 vs. NCBI nr
Match: TYK29457.1 (adenylyl-sulfate kinase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 555.8 bits (1431), Expect = 2.2e-154
Identity = 276/295 (93.56%), Postives = 279/295 (94.58%), Query Frame = 0

Query: 1   MVAIGGISLPGFVPPSLHHQSFSPSAIGFARFPDRISFRSLVVLRDGVEKKGSRVVIVNG 60
           MV+IG ISLPG VPPSLHHQ   PSAIGFARFPDRISFRS VVLRD VEKKGSRVVIVNG
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  KVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLKQ 120
           KVDGLGKSECETEFDT LGNG AGNSGKNHGVLSTVGNSTNIKWHECSVGK EKQSLLKQ
Sbjct: 61  KVDGLGKSECETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIW TGLSGSGKSTVACALSQSL +MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDTKG 240
           RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARD KG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGFPCEMAEKVLSYLEQKGFLQ 296
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH GG P EMAEKVL+YLEQKGFLQ
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQ 295

BLAST of Lsi03G010160 vs. NCBI nr
Match: XP_031739526.1 (adenylyl-sulfate kinase 3 [Cucumis sativus] >KGN52751.1 hypothetical protein Csa_014768 [Cucumis sativus])

HSP 1 Score: 551.2 bits (1419), Expect = 5.4e-153
Identity = 272/296 (91.89%), Postives = 278/296 (93.92%), Query Frame = 0

Query: 1   MVAIGGISLPGFVPPSLHHQSFSPSAIGFARFPDRISFRSLVVLRDGVEKKGSRVVIVNG 60
           MV+IGGISLPG +PP LHHQS   SAIGFARFPDRISFRS VVLRD VEKKGSRVVI NG
Sbjct: 1   MVSIGGISLPGSIPPLLHHQSSPTSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIANG 60

Query: 61  KVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLKQ 120
           KVDGLGKSECET+FDT LGNG AGNSGKNHGVLSTVGNSTNIKWHECSVGK EKQSLLKQ
Sbjct: 61  KVDGLGKSECETDFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLLKQ 120

Query: 121 KGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIW TGLSGSGKSTVACALSQSLY+MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWFTGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDTKG 240
           RRVGEVAKLFADAGVICIASLISPYRR+RDACR ILP+GYFIEVFMDVPLEVCEARD KG
Sbjct: 181 RRVGEVAKLFADAGVICIASLISPYRRERDACRDILPNGYFIEVFMDVPLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGFPCEMAEKVLSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVP NCEIVLKH GG P EMAEKVLSYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPFNCEIVLKHTGGSPSEMAEKVLSYLEQKGFLQA 296

BLAST of Lsi03G010160 vs. NCBI nr
Match: KAG6576911.1 (Adenylyl-sulfate kinase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014937.1 Adenylyl-sulfate kinase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 531.6 bits (1368), Expect = 4.4e-147
Identity = 263/296 (88.85%), Postives = 272/296 (91.89%), Query Frame = 0

Query: 1   MVAIGGISLPGFVPPSLHHQSFSPSAIGFARFPDRISFRSLVVLRDGVEKKGSRVVIVNG 60
           MVAIGGISLP FV PSLH QSFSP AIGFARFPDRI  RS V L D VEK GSR  IVN 
Sbjct: 1   MVAIGGISLPRFVAPSLHRQSFSPPAIGFARFPDRIRLRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  KVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLKQ 120
           KVDGL KS+CET+FD TL NGHA NSGKN GVLSTVGNSTNIKWHECSVGK+E+QSLL+Q
Sbjct: 61  KVDGLVKSDCETDFDATLSNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKS VACAL+Q LY+MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEVCEARDTKG 240
           RR+GEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDV LEVCEARD KG
Sbjct: 181 RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVSLEVCEARDAKG 240

Query: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGFPCEMAEKVLSYLEQKGFLQA 297
           LYKLARAGKIKGFTGIDDPYEVPLNCEIVLK+NGG PCEMAEKVLSYLEQKGFLQA
Sbjct: 241 LYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVLSYLEQKGFLQA 296

BLAST of Lsi03G010160 vs. TAIR 10
Match: AT2G14750.1 (APS kinase )

HSP 1 Score: 363.2 bits (931), Expect = 1.9e-100
Identity = 187/259 (72.20%), Postives = 208/259 (80.31%), Query Frame = 0

Query: 39  RSLVVLRDGVEKKGSRVVIVNGKVDGLGKSECETEFDTTLGNGHAGNSGKNHGVLSTVGN 98
           RS+VV+R  V   GS+ +  N       K+    E  +   NGH   +G+  G LSTVGN
Sbjct: 30  RSVVVVRACVSMDGSQTLSHN-------KNGSIPEVKSI--NGH---TGQKQGPLSTVGN 89

Query: 99  STNIKWHECSVGKVEKQSLLKQKGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYILDG 158
           STNIKWHECSV KV++Q LL QKGCVIW+TGLSGSGKST+ACAL+Q LYQ GKL YILDG
Sbjct: 90  STNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLACALNQMLYQKGKLCYILDG 149

Query: 159 DNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPD 218
           DNVRHGLNRDL FKAEDRAENIRRVGEVAKLFADAG+ICIASLISPYR DRDACR++LP+
Sbjct: 150 DNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIASLISPYRTDRDACRSLLPE 209

Query: 219 GYFIEVFMDVPLEVCEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGG-FP 278
           G F+EVFMDVPL VCEARD KGLYKLARAGKIKGFTGIDDPYE PLNCEI L   GG  P
Sbjct: 210 GDFVEVFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDDPYEPPLNCEISLGREGGTSP 269

Query: 279 CEMAEKVLSYLEQKGFLQA 297
            EMAEKV+ YL+ KG+LQA
Sbjct: 270 IEMAEKVVGYLDNKGYLQA 276

BLAST of Lsi03G010160 vs. TAIR 10
Match: AT3G03900.1 (adenosine-5'-phosphosulfate (APS) kinase 3 )

HSP 1 Score: 322.8 bits (826), Expect = 2.9e-88
Identity = 155/206 (75.24%), Postives = 176/206 (85.44%), Query Frame = 0

Query: 93  LSTVGNSTNIKWHECSVGKVEKQSLLKQKGCVIWITGLSGSGKSTVACALSQSLYQMGKL 152
           +STVGNSTNI W E  +GK E+Q LL QKGCV+WITGLSGSGKST+AC+LS+ L   GKL
Sbjct: 1   MSTVGNSTNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKL 60

Query: 153 AYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDAC 212
           +YILDGDN+RHGLN+DLGFKAEDR ENIRRVGEVAKLFADAG+ICIASLISPYR+DRDAC
Sbjct: 61  SYILDGDNLRHGLNKDLGFKAEDRVENIRRVGEVAKLFADAGLICIASLISPYRKDRDAC 120

Query: 213 RAILPDGYFIEVFMDVPLEVCEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH 272
           R ++ +  FIEVFM++ L++CEARD KGLYKLARAGKIKGFTGIDDPYE PLNCEI LK 
Sbjct: 121 REMIQNSSFIEVFMNMSLQLCEARDPKGLYKLARAGKIKGFTGIDDPYESPLNCEIELKE 180

Query: 273 NGG---FPCEMAEKVLSYLEQKGFLQ 296
             G    P  MAE+V+SYLE KGFLQ
Sbjct: 181 KEGECPSPVAMAEEVISYLEDKGFLQ 206

BLAST of Lsi03G010160 vs. TAIR 10
Match: AT4G39940.1 (APS-kinase 2 )

HSP 1 Score: 322.8 bits (826), Expect = 2.9e-88
Identity = 153/213 (71.83%), Postives = 179/213 (84.04%), Query Frame = 0

Query: 87  GKNHGVLSTVGNSTNIKWHECSVGKVEKQSLLKQKGCVIWITGLSGSGKSTVACALSQSL 146
           G+N+G       + NI WHE S+ + ++Q LL+QKGCV+WITGLSGSGKSTVACALS++L
Sbjct: 84  GRNNG----KEKAENIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALSKAL 143

Query: 147 YQMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYR 206
           ++ GKL Y LDGDNVRHGLNRDL FKAE R ENIRR+GEVAKLFAD GVICIASLISPYR
Sbjct: 144 FERGKLTYTLDGDNVRHGLNRDLTFKAEHRTENIRRIGEVAKLFADVGVICIASLISPYR 203

Query: 207 RDRDACRAILPDGYFIEVFMDVPLEVCEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNC 266
           RDRDACR++LPDG F+EVFMDVPL VCE+RD KGLYKLARAGKIKGFTGIDDPYE P+NC
Sbjct: 204 RDRDACRSLLPDGDFVEVFMDVPLHVCESRDPKGLYKLARAGKIKGFTGIDDPYEAPVNC 263

Query: 267 EIVLKHNGG----FPCEMAEKVLSYLEQKGFLQ 296
           E+VLKH G      P +MAE ++SYL+ KG+L+
Sbjct: 264 EVVLKHTGDDESCSPRQMAENIISYLQNKGYLE 292

BLAST of Lsi03G010160 vs. TAIR 10
Match: AT5G67520.1 (adenosine-5'-phosphosulfate (APS) kinase 4 )

HSP 1 Score: 303.9 bits (777), Expect = 1.4e-82
Identity = 146/217 (67.28%), Postives = 173/217 (79.72%), Query Frame = 0

Query: 97  GNSTNIKWHECSVGKVEKQSLLKQKGCVIWITGLSGSGKSTVACALSQSLYQMGKLAYIL 156
           G   NI WH+C V K ++Q L+KQKGCVIWITGLSGSGKS++ACALS++L+  GKL+YIL
Sbjct: 84  GKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSLACALSRALHNRGKLSYIL 143

Query: 157 DGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAIL 216
           DGDNVRHGLN DL F+A+DRAENIRRVGEVAKLFAD+G+ICIASLISPYR +R ACRA+L
Sbjct: 144 DGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICIASLISPYRIERAACRALL 203

Query: 217 PDGYFIEVFMDVPLEVCEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH---- 276
           P G FIEVFMDVPL VCEARD KGLYK ARAGKIKGFTG+DDPYE PL+CEIV+++    
Sbjct: 204 PQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSRDK 263

Query: 277 --------------NGGFPCEMAEKVLSYLEQKGFLQ 296
                         +    CEMA+ V+SYL+Q G+L+
Sbjct: 264 GLSSSSSSSSSPSSSSSSLCEMADIVVSYLDQNGYLK 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q432952.7e-9972.20Adenylyl-sulfate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK1... [more]
O492042.7e-9169.80Adenylyl-sulfate kinase, chloroplastic OS=Catharanthus roseus OX=4058 GN=AKN PE=... [more]
O491964.1e-8771.83Adenylyl-sulfate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK2... [more]
Q9SRW74.1e-8775.24Adenylyl-sulfate kinase 3 OS=Arabidopsis thaliana OX=3702 GN=APK3 PE=1 SV=1[more]
Q84JF02.0e-8167.28Adenylyl-sulfate kinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK4... [more]
Match NameE-valueIdentityDescription
A0A1S3BYH93.7e-15593.58Adenylyl-sulfate kinase OS=Cucumis melo OX=3656 GN=LOC103494812 PE=3 SV=1[more]
A0A5A7TN803.7e-15593.58Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5D3DZZ61.1e-15493.56Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A0A0KY242.6e-15391.89Adenylyl-sulfate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000720 PE=3 SV=1[more]
A0A6J1J8X72.8e-14787.21Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482821 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038903366.14.1e-16196.28adenylyl-sulfate kinase 3-like [Benincasa hispida][more]
XP_008454396.17.6e-15593.58PREDICTED: adenylyl-sulfate kinase 3 [Cucumis melo] >XP_008454397.1 PREDICTED: a... [more]
TYK29457.12.2e-15493.56adenylyl-sulfate kinase 3 [Cucumis melo var. makuwa][more]
XP_031739526.15.4e-15391.89adenylyl-sulfate kinase 3 [Cucumis sativus] >KGN52751.1 hypothetical protein Csa... [more]
KAG6576911.14.4e-14788.85Adenylyl-sulfate kinase 1, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
AT2G14750.11.9e-10072.20APS kinase [more]
AT3G03900.12.9e-8875.24adenosine-5'-phosphosulfate (APS) kinase 3 [more]
AT4G39940.12.9e-8871.83APS-kinase 2 [more]
AT5G67520.11.4e-8267.28adenosine-5'-phosphosulfate (APS) kinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01583APS_kinasecoord: 121..273
e-value: 3.0E-72
score: 241.7
NoneNo IPR availablePANTHERPTHR11055:SF55ADENYLYL-SULFATE KINASE 1, CHLOROPLASTICcoord: 59..296
NoneNo IPR availablePANTHERPTHR11055BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASEcoord: 59..296
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 93..296
e-value: 1.7E-92
score: 310.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 120..291
IPR002891Adenylyl-sulfate kinaseTIGRFAMTIGR00455TIGR00455coord: 104..275
e-value: 1.6E-75
score: 250.8
IPR002891Adenylyl-sulfate kinaseHAMAPMF_00065Adenylyl_sulf_kinasecoord: 120..292
score: 36.023602
IPR002891Adenylyl-sulfate kinaseCDDcd02027APSKcoord: 124..271
e-value: 2.47062E-101
score: 291.304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G010160.1Lsi03G010160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070814 hydrogen sulfide biosynthetic process
biological_process GO:0016310 phosphorylation
biological_process GO:0000103 sulfate assimilation
molecular_function GO:0004020 adenylylsulfate kinase activity
molecular_function GO:0005524 ATP binding