Lsi03G009480 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G009480
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionUbiquinol oxidase
Locationchr03: 18128187 .. 18130745 (-)
RNA-Seq ExpressionLsi03G009480
SyntenyLsi03G009480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATAAATCCTTTCATTCTTGCTCCCCAAGAAACTGGACTTCCTGGAACCTTCTCGAAGCTCGACGCCTTACGAGAGCCGTCGAAGATTTCTTGAGCCCCAAGCAAAGTCATAATTACCAACTTGATCAACCGTCGCACTGAAACTGCGAAGCACAGGATTATAATCCTTTGTCGGTTACTTTCACCAAACACAGCCCATTACTTATAACGGACTGGTCATTGCTTTCATGGACAATCGGAGGAGAATCGACGTGAAGGAATTTGGTCCATTTGTTTTGATTTCTAGAGATGAATCGGATTGTGATTAGGAGTTTTGTGAGGGGTTTGGTTAATAGCCGGAGCTTTGGCTCCGTCGCCGGAGTTGCCGGTGGTGGCAGTGGCGGAGGTATGAAGTTTACTTTCGCGGCGGTGGCGTCGCCGGGGACGAGGTATCAGCCGATGGTGGATGGCGGATTCCAGTGGAGGAGGTTGTTGAGCTCGTCGACGGCGGTGGCGGAGGAACAAGGGAAGGTGAAATTAGAACAGGGGATTAAGGATATTGAGAATCAGGAGAAGAAGGAAAAAGAGAAGGAGAATGCTCTAGTTTCTAGTTATTGGGGGATTTACAGGCCGAAGATTACTAGAGAAGATGGGTCTGAATGGCCTTGGAATTGCTTTATGGTATGAATTTGGTCTTCGCTTTATTTGCCCTTTTCAATGTTCTGTTTTATTGACTTTAGTGGGAGCTTCGAATTTGATTGTTGTACTCTTGCCCGTCAACTGTTTGTTAAAATGCTTGCTTGCTTTTGGCTTTAAATTGTGTGTCCTTTTTTGGGGTAATTCGATATTCTTTGAAGTTTGCCTGATTTGGCTTTGGGAGGTGCGATTAAACGTTTTATAATCCAGTGTTTTGATCAAATCCTTCTTGTTAAGCAAGAGAATGGAGTTTCTCCAACTGTTTTTTTCTTCTTAATGAGTTGTGGTTCTGAAGAAACTGTTGCTGTAAATGCAGCCATGGGAGACTTACAGAGCAGATGTTTCCATAGATCTTGGAAAGCACCATGAGCCGAAAACCTTTCTGGACAAAGTCGCTTACCGCGTTGTAAAGCTTCTTCGGATCCCAACAGATATCTTCTTCCAGGTGGGTTATCTGATTTGTTTGTTTGCTCTTCCTTCAGAGCCCTTGACTTAGAACACTGAATTCTGAGTGATTCAATCAATTTTTGAAAACCCTTCTTACAAAAAACAATCGGATGATTCTAAATGTGGAAAGTTCCTCTGGTCAACACAATGTGTTGCCCTTTCTTTAGGATTCAGATTTGAAAATCCAACTAACTATTCAATACAAGTTTAATAGTGTAGGATTTGTTGTTATGGCCCATGAATAGTTTCACCTTAATGTGCGAGTTTCTGTTGTACATTGTCTAGAATTCATTTCAGTTTCCATGTTTGTGCAGAGACGTTACGGTTGTCGAGCGGTGATGCTTGAGACAGTGGCAGCGGTTCCGGGGATGGTTGGAGGGATGCTGTTGCATCTGAAGTCTCTGAGGAAGTTTCAGCACAGTGGGGGGTGGATTAAGGCCCTGCTTGAAGAAGCAGAGAATGAGAGGATGCATTTAATGACCATGATTGAGCTTGTGCAGCCCAAGTGGTACGAACGGCTGCTTGTGATCACAGTGCAGGGAGTGTTTTTCAATGCCTTCTTTGTGCTCTACTTGATGTCCCCCAAATTGGCTCATAGAATTGTTGGCTATTTGGAAGAGGAAGCCATCCATTCTTACACTGAGTACTTGAAGGATATCGACGAAGGAAAGATCGAAAACGTCCCCGCTCCTGCCATCGCCATCGACTATTGGAGGCTGCCTAAGGATGCAAGGCTGAAGGATGTTATCACAGTCATTCGGGCCGATGAGGCGCACCACCGCGACGTCAACCATTTTGCTTCTGTGAGTAACAATGCTTCCTTTTCTGTTCACATACTTTACAAAGTATACTCACTAAATTTTAATATTGTGTCCACAAGGTACCAAACTTTAAATCGTCTAATAGGTGTTGACGAACAATTATTCTATCAATTTTAAAGTGTAGTAGATCCATTGATTTTATAAAATGTCGAATATGTCATACCTATTAGATATAAAATTGAAATTTCAATGAATGATCTATTGGACATCTCAGAATACAATGGTTATGTTTTTAACATGGTTTTTTTTAGAAGTCAAGTATTGAACGGCATGGCTTTTTTTTATGTGCAGGACATCCATTTCCAAGGGAAGGAATTGAGAGAATCAGCAGCCCCTCTGGGATATCACTAAACTCATATTCTTTTGTGTCCTTTTGGATTATAGCTTTAAGAGAGAGTACTTTGTTTTGTGTTGTTGAGATGTACAAGTGGGAAATGGCTTAATTTGGTCTTAATAACTTCTAGTAAATGTGTCGTAATTTGTACATAGGTTCTCTCTTGAATTTACATCCAATAAAGACCTTCATAAGTTTTCTAGGCATATGGTTATGATTGTTGGATTTCACTTGTAGAAATGATGATTGTATTTGACTATACTTTAGAAGTTATGTATTTTC

mRNA sequence

TATAAATCCTTTCATTCTTGCTCCCCAAGAAACTGGACTTCCTGGAACCTTCTCGAAGCTCGACGCCTTACGAGAGCCGTCGAAGATTTCTTGAGCCCCAAGCAAAGTCATAATTACCAACTTGATCAACCGTCGCACTGAAACTGCGAAGCACAGGATTATAATCCTTTGTCGGTTACTTTCACCAAACACAGCCCATTACTTATAACGGACTGGTCATTGCTTTCATGGACAATCGGAGGAGAATCGACGTGAAGGAATTTGGTCCATTTGTTTTGATTTCTAGAGATGAATCGGATTGTGATTAGGAGTTTTGTGAGGGGTTTGGTTAATAGCCGGAGCTTTGGCTCCGTCGCCGGAGTTGCCGGTGGTGGCAGTGGCGGAGGTATGAAGTTTACTTTCGCGGCGGTGGCGTCGCCGGGGACGAGGTATCAGCCGATGGTGGATGGCGGATTCCAGTGGAGGAGGTTGTTGAGCTCGTCGACGGCGGTGGCGGAGGAACAAGGGAAGGTGAAATTAGAACAGGGGATTAAGGATATTGAGAATCAGGAGAAGAAGGAAAAAGAGAAGGAGAATGCTCTAGTTTCTAGTTATTGGGGGATTTACAGGCCGAAGATTACTAGAGAAGATGGGTCTGAATGGCCTTGGAATTGCTTTATGCCATGGGAGACTTACAGAGCAGATGTTTCCATAGATCTTGGAAAGCACCATGAGCCGAAAACCTTTCTGGACAAAGTCGCTTACCGCGTTGTAAAGCTTCTTCGGATCCCAACAGATATCTTCTTCCAGAGACGTTACGGTTGTCGAGCGGTGATGCTTGAGACAGTGGCAGCGGTTCCGGGGATGGTTGGAGGGATGCTGTTGCATCTGAAGTCTCTGAGGAAGTTTCAGCACAGTGGGGGGTGGATTAAGGCCCTGCTTGAAGAAGCAGAGAATGAGAGGATGCATTTAATGACCATGATTGAGCTTGTGCAGCCCAAGTGGTACGAACGGCTGCTTGTGATCACAGTGCAGGGAGTGTTTTTCAATGCCTTCTTTGTGCTCTACTTGATGTCCCCCAAATTGGCTCATAGAATTGTTGGCTATTTGGAAGAGGAAGCCATCCATTCTTACACTGAGTACTTGAAGGATATCGACGAAGGAAAGATCGAAAACGTCCCCGCTCCTGCCATCGCCATCGACTATTGGAGGCTGCCTAAGGATGCAAGGCTGAAGGATGTTATCACAGTCATTCGGGCCGATGAGGCGCACCACCGCGACGTCAACCATTTTGCTTCTGACATCCATTTCCAAGGGAAGGAATTGAGAGAATCAGCAGCCCCTCTGGGATATCACTAAACTCATATTCTTTTGTGTCCTTTTGGATTATAGCTTTAAGAGAGAGTACTTTGTTTTGTGTTGTTGAGATGTACAAGTGGGAAATGGCTTAATTTGGTCTTAATAACTTCTAGTAAATGTGTCGTAATTTGTACATAGGTTCTCTCTTGAATTTACATCCAATAAAGACCTTCATAAGTTTTCTAGGCATATGGTTATGATTGTTGGATTTCACTTGTAGAAATGATGATTGTATTTGACTATACTTTAGAAGTTATGTATTTTC

Coding sequence (CDS)

ATGAATCGGATTGTGATTAGGAGTTTTGTGAGGGGTTTGGTTAATAGCCGGAGCTTTGGCTCCGTCGCCGGAGTTGCCGGTGGTGGCAGTGGCGGAGGTATGAAGTTTACTTTCGCGGCGGTGGCGTCGCCGGGGACGAGGTATCAGCCGATGGTGGATGGCGGATTCCAGTGGAGGAGGTTGTTGAGCTCGTCGACGGCGGTGGCGGAGGAACAAGGGAAGGTGAAATTAGAACAGGGGATTAAGGATATTGAGAATCAGGAGAAGAAGGAAAAAGAGAAGGAGAATGCTCTAGTTTCTAGTTATTGGGGGATTTACAGGCCGAAGATTACTAGAGAAGATGGGTCTGAATGGCCTTGGAATTGCTTTATGCCATGGGAGACTTACAGAGCAGATGTTTCCATAGATCTTGGAAAGCACCATGAGCCGAAAACCTTTCTGGACAAAGTCGCTTACCGCGTTGTAAAGCTTCTTCGGATCCCAACAGATATCTTCTTCCAGAGACGTTACGGTTGTCGAGCGGTGATGCTTGAGACAGTGGCAGCGGTTCCGGGGATGGTTGGAGGGATGCTGTTGCATCTGAAGTCTCTGAGGAAGTTTCAGCACAGTGGGGGGTGGATTAAGGCCCTGCTTGAAGAAGCAGAGAATGAGAGGATGCATTTAATGACCATGATTGAGCTTGTGCAGCCCAAGTGGTACGAACGGCTGCTTGTGATCACAGTGCAGGGAGTGTTTTTCAATGCCTTCTTTGTGCTCTACTTGATGTCCCCCAAATTGGCTCATAGAATTGTTGGCTATTTGGAAGAGGAAGCCATCCATTCTTACACTGAGTACTTGAAGGATATCGACGAAGGAAAGATCGAAAACGTCCCCGCTCCTGCCATCGCCATCGACTATTGGAGGCTGCCTAAGGATGCAAGGCTGAAGGATGTTATCACAGTCATTCGGGCCGATGAGGCGCACCACCGCGACGTCAACCATTTTGCTTCTGACATCCATTTCCAAGGGAAGGAATTGAGAGAATCAGCAGCCCCTCTGGGATATCACTAA

Protein sequence

MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKFTFAAVASPGTRYQPMVDGGFQWRRLLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWPWNCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETVAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH
Homology
BLAST of Lsi03G009480 vs. ExPASy Swiss-Prot
Match: Q40294 (Ubiquinol oxidase, mitochondrial OS=Mangifera indica OX=29780 GN=AOMI 1 PE=1 SV=2)

HSP 1 Score: 479.2 bits (1232), Expect = 4.0e-134
Identity = 236/332 (71.08%), Postives = 277/332 (83.43%), Query Frame = 0

Query: 24  GVAGGGSGGGMKFTFAAVASPGTRYQPMVDGG------FQWRRLLSSSTAVAEEQGKVKL 83
           G+  GG  G  ++ + A++    R+  +++G        QWRR+LS++            
Sbjct: 6   GLLNGGRYGN-RYIWTAIS---LRHPEVMEGNGLESAVMQWRRMLSNA------------ 65

Query: 84  EQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWPWNCFMPWETYRADVSIDL 143
             G  + + +E+KE EK++A+VS+YWGI RPKITREDGSEWPWNCFMPWETYR+D+SIDL
Sbjct: 66  --GGAEAQVKEQKE-EKKDAMVSNYWGISRPKITREDGSEWPWNCFMPWETYRSDLSIDL 125

Query: 144 GKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETVAAVPGMVGGMLLHLKSL 203
            KHH P+TF+DK AYR VK+LR+PTDIFFQRRYGCRA+MLETVAAVPGMVGGMLLHLKSL
Sbjct: 126 KKHHVPRTFMDKFAYRTVKILRVPTDIFFQRRYGCRAMMLETVAAVPGMVGGMLLHLKSL 185

Query: 204 RKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVITVQGVFFNAFFVLYLMSP 263
           RK + SGGWIKALLEEAENERMHLMTM+ELVQPKWYERLLV+ VQGVFFN+FFVLY++SP
Sbjct: 186 RKLEQSGGWIKALLEEAENERMHLMTMVELVQPKWYERLLVLAVQGVFFNSFFVLYVLSP 245

Query: 264 KLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYWRLPKDARLKDVITVIRA 323
           KLAHRIVGYLEEEAIHSYTEYLKDID G I+N+PAPAIAIDYWRLPKDA LKDVITV+RA
Sbjct: 246 KLAHRIVGYLEEEAIHSYTEYLKDIDSGAIKNIPAPAIAIDYWRLPKDATLKDVITVVRA 305

Query: 324 DEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           DEAHHRDVNHFASD+  QGKELR++ AP+GYH
Sbjct: 306 DEAHHRDVNHFASDVQVQGKELRDAPAPVGYH 318

BLAST of Lsi03G009480 vs. ExPASy Swiss-Prot
Match: Q41266 (Ubiquinol oxidase 2, mitochondrial OS=Glycine max OX=3847 GN=AOX2 PE=1 SV=2)

HSP 1 Score: 478.4 bits (1230), Expect = 6.9e-134
Identity = 227/294 (77.21%), Postives = 257/294 (87.41%), Query Frame = 0

Query: 56  FQWRRLLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDG 115
           F W+R + S             E  + + E +++K K +++ + SSYWGI RPK+ REDG
Sbjct: 51  FYWKRTMVSPA-----------EAKVPEKEKEKEKAKAEKSVVESSYWGISRPKVVREDG 110

Query: 116 SEWPWNCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 175
           +EWPWNCFMPWE+YR++VSIDL KHH PK  LDKVAYR VKLLRIPTD+FF+RRYGCRA+
Sbjct: 111 TEWPWNCFMPWESYRSNVSIDLTKHHVPKNVLDKVAYRTVKLLRIPTDLFFKRRYGCRAM 170

Query: 176 MLETVAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 235
           MLETVAAVPGMVGGMLLHL+SLRKFQ SGGWIKALLEEAENERMHLMTM+ELV+PKWYER
Sbjct: 171 MLETVAAVPGMVGGMLLHLRSLRKFQQSGGWIKALLEEAENERMHLMTMVELVKPKWYER 230

Query: 236 LLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAI 295
           LLV+ VQGVFFNAFFVLY++SPK+AHRIVGYLEEEAIHSYTEYLKD++ G IENVPAPAI
Sbjct: 231 LLVLAVQGVFFNAFFVLYILSPKVAHRIVGYLEEEAIHSYTEYLKDLESGAIENVPAPAI 290

Query: 296 AIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           AIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRE+ AP+GYH
Sbjct: 291 AIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELREAPAPIGYH 333

BLAST of Lsi03G009480 vs. ExPASy Swiss-Prot
Match: O22049 (Ubiquinol oxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX2 PE=1 SV=2)

HSP 1 Score: 453.8 bits (1166), Expect = 1.8e-126
Identity = 221/301 (73.42%), Postives = 257/301 (85.38%), Query Frame = 0

Query: 52  VDGGFQWRRL-LSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGI--YRP 111
           V G F  R + +SS++A+ ++   + +++G          +    +  V SYWGI   + 
Sbjct: 63  VQGNFNLRWMGMSSASAMEKKDENLTVKKG----------QNGGGSVAVPSYWGIETAKM 122

Query: 112 KITREDGSEWPWNCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQR 171
           KITR+DGS+WPWNCFMPWETY+A++SIDL KHH PK   DKVAYR+VKLLRIPTDIFFQR
Sbjct: 123 KITRKDGSDWPWNCFMPWETYQANLSIDLKKHHVPKNIADKVAYRIVKLLRIPTDIFFQR 182

Query: 172 RYGCRAVMLETVAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELV 231
           RYGCRA+MLETVAAVPGMVGGMLLHLKS+RKF+HSGGWIKALLEEAENERMHLMTM+ELV
Sbjct: 183 RYGCRAMMLETVAAVPGMVGGMLLHLKSIRKFEHSGGWIKALLEEAENERMHLMTMMELV 242

Query: 232 QPKWYERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIE 291
           +PKWYERLLV+ VQG+FFN+FFV Y++SP+LAHR+VGYLEEEAIHSYTE+LKDID GKIE
Sbjct: 243 KPKWYERLLVMLVQGIFFNSFFVCYVISPRLAHRVVGYLEEEAIHSYTEFLKDIDNGKIE 302

Query: 292 NVPAPAIAIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGY 350
           NV APAIAIDYWRLPKDA LKDV+TVIRADEAHHRDVNHFASDI  QGKELRE+AAP+GY
Sbjct: 303 NVAAPAIAIDYWRLPKDATLKDVVTVIRADEAHHRDVNHFASDIRNQGKELREAAAPIGY 353

BLAST of Lsi03G009480 vs. ExPASy Swiss-Prot
Match: O03376 (Alternative oxidase 3, mitochondrial OS=Glycine max OX=3847 GN=AOX3 PE=1 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 7.1e-123
Identity = 219/351 (62.39%), Postives = 265/351 (75.50%), Query Frame = 0

Query: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKFTFAAVASPGTRYQPMVDGGFQWRR 60
           M  +++RS  R L           + GGG     + + AA+     ++     G F  RR
Sbjct: 1   MKNVLVRSAARAL-----------LGGGGRSYYRQLSTAAIVEQRHQHGGGAFGSFHLRR 60

Query: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEK--ENALVSSYWGIYRPKITREDGSEW 120
           +    + + E          +KD  ++EKK +     NA+V+SYWGI RPK+ REDG+EW
Sbjct: 61  M----STLPE----------VKDQHSEEKKNEVNGTSNAVVTSYWGITRPKVRREDGTEW 120

Query: 121 PWNCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLE 180
           PWNCFMPW++Y +DVSID+ KHH PK+  DKVA+R VK LR+ +DI+F+ RYGC A+MLE
Sbjct: 121 PWNCFMPWDSYHSDVSIDVTKHHTPKSLTDKVAFRAVKFLRVLSDIYFKERYGCHAMMLE 180

Query: 181 TVAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLV 240
           T+AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTM+ELV+P W+ERLL+
Sbjct: 181 TIAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMVELVKPSWHERLLI 240

Query: 241 ITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAID 300
            T QGVFFNAFFV YL+SPK AHR VGYLEEEA+ SYT++L  I+ GK+ENVPAPAIAID
Sbjct: 241 FTAQGVFFNAFFVFYLLSPKAAHRFVGYLEEEAVISYTQHLNAIESGKVENVPAPAIAID 300

Query: 301 YWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           YWRLPKDA LKDV+TVIRADEAHHRDVNHFASDIH QGKEL+E+ AP+GYH
Sbjct: 301 YWRLPKDATLKDVVTVIRADEAHHRDVNHFASDIHHQGKELKEAPAPIGYH 326

BLAST of Lsi03G009480 vs. ExPASy Swiss-Prot
Match: O82807 (Ubiquinol oxidase 1a, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=AOX1A PE=2 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 4.3e-120
Identity = 214/327 (65.44%), Postives = 255/327 (77.98%), Query Frame = 0

Query: 32  GGMKFTFAAVASP----GTRYQPMVDGGFQ----WRRLLSSSTAVAEEQGKVKLEQGIKD 91
           GG++   A+  SP        +P + GG      W   L S+++VA  +   K E     
Sbjct: 15  GGVRLFTASATSPAAAAAAAARPFLAGGEAVPGVWGLRLMSTSSVASTEAAAKAE----- 74

Query: 92  IENQEKKEKEKENALVSSYWGIYR-PKITREDGSEWPWNCFMPWETYRADVSIDLGKHHE 151
               +K + EKE  +V+SYWGI +  K+ REDG+EW W+CF PWETY AD SIDL KHH 
Sbjct: 75  ---AKKADAEKE-VVVNSYWGIEQSKKLVREDGTEWKWSCFRPWETYTADTSIDLTKHHV 134

Query: 152 PKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETVAAVPGMVGGMLLHLKSLRKFQH 211
           PKT LDK+AY  VK LR PTDIFFQRRYGCRA+MLETVAAVPGMVGGMLLHL+SLR+F+ 
Sbjct: 135 PKTLLDKIAYWTVKSLRFPTDIFFQRRYGCRAMMLETVAAVPGMVGGMLLHLRSLRRFEQ 194

Query: 212 SGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVITVQGVFFNAFFVLYLMSPKLAHR 271
           SGGWI+ LLEEAENERMHLMT +E+  PKWYER LVITVQGVFFNA+F+ YL+SPK AHR
Sbjct: 195 SGGWIRTLLEEAENERMHLMTFMEVANPKWYERALVITVQGVFFNAYFLGYLLSPKFAHR 254

Query: 272 IVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYWRLPKDARLKDVITVIRADEAHH 331
           +VGYLEEEAIHSYTE+LKD++ GKI+NVPAPAIAIDYWRLP +A LKDV+TV+RADEAHH
Sbjct: 255 VVGYLEEEAIHSYTEFLKDLEAGKIDNVPAPAIAIDYWRLPANATLKDVVTVVRADEAHH 314

Query: 332 RDVNHFASDIHFQGKELRESAAPLGYH 350
           RDVNHFASDIH+QG EL+++ AP+GYH
Sbjct: 315 RDVNHFASDIHYQGMELKQTPAPIGYH 332

BLAST of Lsi03G009480 vs. ExPASy TrEMBL
Match: G3G3W9 (Ubiquinol oxidase OS=Citrullus lanatus OX=3654 PE=3 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 3.9e-196
Identity = 345/349 (98.85%), Postives = 346/349 (99.14%), Query Frame = 0

Query: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKFTFAAVASPGTRYQPMVDGGFQWRR 60
           MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMK TFAAVASPGTRYQPMVDGGFQWRR
Sbjct: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKVTFAAVASPGTRYQPMVDGGFQWRR 60

Query: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWPW 120
           LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKE ENALVSSYWGIYRPKITREDGSEWPW
Sbjct: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKETENALVSSYWGIYRPKITREDGSEWPW 120

Query: 121 NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180
           NCFMPWETYRAD+SIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV
Sbjct: 121 NCFMPWETYRADLSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180

Query: 181 AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240
           AAVPGMVGGMLLHLKSLRKFQHSG WIKALLEEAENERMHLMTMIELVQPKWYERLLVIT
Sbjct: 181 AAVPGMVGGMLLHLKSLRKFQHSGVWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240

Query: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300
           VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW
Sbjct: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300

Query: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH
Sbjct: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 349

BLAST of Lsi03G009480 vs. ExPASy TrEMBL
Match: D4P4J8 (Ubiquinol oxidase OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 8.6e-196
Identity = 343/349 (98.28%), Postives = 344/349 (98.57%), Query Frame = 0

Query: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKFTFAAVASPGTRYQPMVDGGFQWRR 60
           MNRI IRSFVRGLVNSRSFGSVAGVAGGGSGGGMK TFAAVASPGTRYQPMVDGGFQWRR
Sbjct: 1   MNRIAIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKVTFAAVASPGTRYQPMVDGGFQWRR 60

Query: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWPW 120
           LLSSSTAV EEQGKVKLEQGIKDIENQEKKEKE ENALVSSYWGIYRPKITREDGSEWPW
Sbjct: 61  LLSSSTAVTEEQGKVKLEQGIKDIENQEKKEKETENALVSSYWGIYRPKITREDGSEWPW 120

Query: 121 NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180
           NCFMPWETYRAD+SIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV
Sbjct: 121 NCFMPWETYRADLSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180

Query: 181 AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240
           AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT
Sbjct: 181 AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240

Query: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300
           VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW
Sbjct: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300

Query: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESA PLGYH
Sbjct: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAVPLGYH 349

BLAST of Lsi03G009480 vs. ExPASy TrEMBL
Match: A0A6J1JCS2 (Ubiquinol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111483245 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.3e-188
Identity = 331/349 (94.84%), Postives = 339/349 (97.13%), Query Frame = 0

Query: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKFTFAAVASPGTRYQPMVDGGFQWRR 60
           MNRIVIRSFVRGLVNSRSFGS+A    GGSGGGMK TFAA ASP TRYQP+ DGGFQWRR
Sbjct: 1   MNRIVIRSFVRGLVNSRSFGSIA----GGSGGGMKVTFAAAASPSTRYQPLADGGFQWRR 60

Query: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWPW 120
           L+SSSTAVAEEQGKVKLEQG KD+ENQE KEKEK+NALVSSYWGIYRPKITREDGSEWPW
Sbjct: 61  LMSSSTAVAEEQGKVKLEQGNKDVENQE-KEKEKDNALVSSYWGIYRPKITREDGSEWPW 120

Query: 121 NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180
           NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV
Sbjct: 121 NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180

Query: 181 AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240
           AAVPGMVGGMLLHLKSLR+FQHSGGWIKALLEEAENERMHLMTMIELVQPKWYER+LVIT
Sbjct: 181 AAVPGMVGGMLLHLKSLRRFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERMLVIT 240

Query: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300
           VQGVFFNAFFVLYLMSPKLAHR+VGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW
Sbjct: 241 VQGVFFNAFFVLYLMSPKLAHRVVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300

Query: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH
Sbjct: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 344

BLAST of Lsi03G009480 vs. ExPASy TrEMBL
Match: A0A6J1E6M0 (Ubiquinol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111430458 PE=3 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 1.8e-185
Identity = 326/349 (93.41%), Postives = 336/349 (96.28%), Query Frame = 0

Query: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKFTFAAVASPGTRYQPMVDGGFQWRR 60
           MNR+VIRS VRGLVNSRSFGS+A    G SGGGMK TFAA ASP TRYQP+ DGGFQWRR
Sbjct: 1   MNRLVIRSVVRGLVNSRSFGSIA----GDSGGGMKVTFAAAASPSTRYQPLADGGFQWRR 60

Query: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWPW 120
           L+SSS+AVAEEQGKVKLEQG KD+ENQE KEKEK+NALVSSYWGIYRPKITREDGSEWPW
Sbjct: 61  LMSSSSAVAEEQGKVKLEQGNKDVENQE-KEKEKDNALVSSYWGIYRPKITREDGSEWPW 120

Query: 121 NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180
           NCFMPWETYRADVSIDLGKHHEPK FLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV
Sbjct: 121 NCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180

Query: 181 AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240
           AAVPGMVGGMLLHLKSLR+FQHSGGWIKALLEEAENERMHLMTMIELVQPKWYER+LVIT
Sbjct: 181 AAVPGMVGGMLLHLKSLRRFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERMLVIT 240

Query: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300
           VQGVFFNAFFVLYLMSPKLAHR+VGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW
Sbjct: 241 VQGVFFNAFFVLYLMSPKLAHRVVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300

Query: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH
Sbjct: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 344

BLAST of Lsi03G009480 vs. ExPASy TrEMBL
Match: A0A0A7DJW5 (Ubiquinol oxidase OS=Cucumis melo OX=3656 GN=LOC103503097 PE=2 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.6e-184
Identity = 325/349 (93.12%), Postives = 334/349 (95.70%), Query Frame = 0

Query: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKFTFAAVASPGTRYQPMVDGGFQWRR 60
           MNRIVIRS VRGLV+SRSF SVAGV GG     MK TFAAVASPGTR QP  DGGFQWRR
Sbjct: 1   MNRIVIRSLVRGLVHSRSFSSVAGVPGGAR---MKVTFAAVASPGTRLQPSADGGFQWRR 60

Query: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWPW 120
            L+SSTAVAEEQGKVK EQG+KDIEN ++KEKEK+NALVSSYWGIYRPKITREDGSEWPW
Sbjct: 61  FLNSSTAVAEEQGKVKSEQGVKDIENPQEKEKEKDNALVSSYWGIYRPKITREDGSEWPW 120

Query: 121 NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180
           NCFMPWETYRAD+SIDLGKHHEP+TFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV
Sbjct: 121 NCFMPWETYRADLSIDLGKHHEPRTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180

Query: 181 AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240
           AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT
Sbjct: 181 AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240

Query: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300
           VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDI+EGKIENVPAPAIAIDYW
Sbjct: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDINEGKIENVPAPAIAIDYW 300

Query: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH
Sbjct: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 346

BLAST of Lsi03G009480 vs. NCBI nr
Match: AEN99850.1 (mitochondrial AOX2 [Citrullus lanatus])

HSP 1 Score: 693.7 bits (1789), Expect = 8.0e-196
Identity = 345/349 (98.85%), Postives = 346/349 (99.14%), Query Frame = 0

Query: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKFTFAAVASPGTRYQPMVDGGFQWRR 60
           MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMK TFAAVASPGTRYQPMVDGGFQWRR
Sbjct: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKVTFAAVASPGTRYQPMVDGGFQWRR 60

Query: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWPW 120
           LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKE ENALVSSYWGIYRPKITREDGSEWPW
Sbjct: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKETENALVSSYWGIYRPKITREDGSEWPW 120

Query: 121 NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180
           NCFMPWETYRAD+SIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV
Sbjct: 121 NCFMPWETYRADLSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180

Query: 181 AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240
           AAVPGMVGGMLLHLKSLRKFQHSG WIKALLEEAENERMHLMTMIELVQPKWYERLLVIT
Sbjct: 181 AAVPGMVGGMLLHLKSLRKFQHSGVWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240

Query: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300
           VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW
Sbjct: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300

Query: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH
Sbjct: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 349

BLAST of Lsi03G009480 vs. NCBI nr
Match: ADD84880.1 (mitochondrial AOX2 [Citrullus lanatus])

HSP 1 Score: 692.6 bits (1786), Expect = 1.8e-195
Identity = 343/349 (98.28%), Postives = 344/349 (98.57%), Query Frame = 0

Query: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKFTFAAVASPGTRYQPMVDGGFQWRR 60
           MNRI IRSFVRGLVNSRSFGSVAGVAGGGSGGGMK TFAAVASPGTRYQPMVDGGFQWRR
Sbjct: 1   MNRIAIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKVTFAAVASPGTRYQPMVDGGFQWRR 60

Query: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWPW 120
           LLSSSTAV EEQGKVKLEQGIKDIENQEKKEKE ENALVSSYWGIYRPKITREDGSEWPW
Sbjct: 61  LLSSSTAVTEEQGKVKLEQGIKDIENQEKKEKETENALVSSYWGIYRPKITREDGSEWPW 120

Query: 121 NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180
           NCFMPWETYRAD+SIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV
Sbjct: 121 NCFMPWETYRADLSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180

Query: 181 AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240
           AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT
Sbjct: 181 AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240

Query: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300
           VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW
Sbjct: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300

Query: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESA PLGYH
Sbjct: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAVPLGYH 349

BLAST of Lsi03G009480 vs. NCBI nr
Match: XP_038876701.1 (ubiquinol oxidase, mitochondrial [Benincasa hispida])

HSP 1 Score: 671.8 bits (1732), Expect = 3.2e-189
Identity = 334/350 (95.43%), Postives = 339/350 (96.86%), Query Frame = 0

Query: 1   MNRIVIRSFVRGLV-NSRSFGSVAGVAGGGSGGGMKFTFAAVASPGTRYQPMVDGGFQWR 60
           MNRIVIRSF R LV NSRSFGSVAGV+ GGS G MK TFAAVASPGTRYQP+ DGGFQWR
Sbjct: 1   MNRIVIRSFARALVNNSRSFGSVAGVSVGGSDGAMKVTFAAVASPGTRYQPLADGGFQWR 60

Query: 61  RLLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWP 120
           R LSSS AVAEEQGKVK+EQGIKDIENQEKKEKEKENA+VSSYWGIYRPKITREDGSEWP
Sbjct: 61  RFLSSSAAVAEEQGKVKMEQGIKDIENQEKKEKEKENAMVSSYWGIYRPKITREDGSEWP 120

Query: 121 WNCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLET 180
           WNCFMPWETYRAD+SIDLGKHHEPK FLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLET
Sbjct: 121 WNCFMPWETYRADLSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLET 180

Query: 181 VAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVI 240
           VAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVI
Sbjct: 181 VAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVI 240

Query: 241 TVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDY 300
           TVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDY
Sbjct: 241 TVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDY 300

Query: 301 WRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           WRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH
Sbjct: 301 WRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350

BLAST of Lsi03G009480 vs. NCBI nr
Match: XP_022985158.1 (ubiquinol oxidase, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 667.9 bits (1722), Expect = 4.7e-188
Identity = 331/349 (94.84%), Postives = 339/349 (97.13%), Query Frame = 0

Query: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKFTFAAVASPGTRYQPMVDGGFQWRR 60
           MNRIVIRSFVRGLVNSRSFGS+A    GGSGGGMK TFAA ASP TRYQP+ DGGFQWRR
Sbjct: 1   MNRIVIRSFVRGLVNSRSFGSIA----GGSGGGMKVTFAAAASPSTRYQPLADGGFQWRR 60

Query: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWPW 120
           L+SSSTAVAEEQGKVKLEQG KD+ENQE KEKEK+NALVSSYWGIYRPKITREDGSEWPW
Sbjct: 61  LMSSSTAVAEEQGKVKLEQGNKDVENQE-KEKEKDNALVSSYWGIYRPKITREDGSEWPW 120

Query: 121 NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180
           NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV
Sbjct: 121 NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180

Query: 181 AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240
           AAVPGMVGGMLLHLKSLR+FQHSGGWIKALLEEAENERMHLMTMIELVQPKWYER+LVIT
Sbjct: 181 AAVPGMVGGMLLHLKSLRRFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERMLVIT 240

Query: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300
           VQGVFFNAFFVLYLMSPKLAHR+VGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW
Sbjct: 241 VQGVFFNAFFVLYLMSPKLAHRVVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300

Query: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH
Sbjct: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 344

BLAST of Lsi03G009480 vs. NCBI nr
Match: XP_023552001.1 (ubiquinol oxidase, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 663.7 bits (1711), Expect = 8.8e-187
Identity = 328/349 (93.98%), Postives = 338/349 (96.85%), Query Frame = 0

Query: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKFTFAAVASPGTRYQPMVDGGFQWRR 60
           MNRIVIRSFVRGLVNSRSFGS+A    GGSGGGMK TFAA ASP TRYQP+ DGGFQWRR
Sbjct: 1   MNRIVIRSFVRGLVNSRSFGSIA----GGSGGGMKVTFAAAASPSTRYQPLADGGFQWRR 60

Query: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWPW 120
           L+SSS+AVAEE GKVKLEQG KD+ENQE KEKEK+NALVSSYWGIYRPKITREDGSEWPW
Sbjct: 61  LMSSSSAVAEEHGKVKLEQGNKDVENQE-KEKEKDNALVSSYWGIYRPKITREDGSEWPW 120

Query: 121 NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180
           NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV
Sbjct: 121 NCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETV 180

Query: 181 AAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVIT 240
           AAVPGMVGGMLLHLKSLR+FQHSGGWIKALLEEAENERMHLMTMIELVQPKWYER+LVIT
Sbjct: 181 AAVPGMVGGMLLHLKSLRRFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERMLVIT 240

Query: 241 VQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300
           VQGVFFNAFFVLYLMSPKLAHR+VGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW
Sbjct: 241 VQGVFFNAFFVLYLMSPKLAHRVVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYW 300

Query: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAP+GYH
Sbjct: 301 RLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPIGYH 344

BLAST of Lsi03G009480 vs. TAIR 10
Match: AT5G64210.1 (alternative oxidase 2 )

HSP 1 Score: 453.8 bits (1166), Expect = 1.3e-127
Identity = 221/301 (73.42%), Postives = 257/301 (85.38%), Query Frame = 0

Query: 52  VDGGFQWRRL-LSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGI--YRP 111
           V G F  R + +SS++A+ ++   + +++G          +    +  V SYWGI   + 
Sbjct: 63  VQGNFNLRWMGMSSASAMEKKDENLTVKKG----------QNGGGSVAVPSYWGIETAKM 122

Query: 112 KITREDGSEWPWNCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQR 171
           KITR+DGS+WPWNCFMPWETY+A++SIDL KHH PK   DKVAYR+VKLLRIPTDIFFQR
Sbjct: 123 KITRKDGSDWPWNCFMPWETYQANLSIDLKKHHVPKNIADKVAYRIVKLLRIPTDIFFQR 182

Query: 172 RYGCRAVMLETVAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELV 231
           RYGCRA+MLETVAAVPGMVGGMLLHLKS+RKF+HSGGWIKALLEEAENERMHLMTM+ELV
Sbjct: 183 RYGCRAMMLETVAAVPGMVGGMLLHLKSIRKFEHSGGWIKALLEEAENERMHLMTMMELV 242

Query: 232 QPKWYERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIE 291
           +PKWYERLLV+ VQG+FFN+FFV Y++SP+LAHR+VGYLEEEAIHSYTE+LKDID GKIE
Sbjct: 243 KPKWYERLLVMLVQGIFFNSFFVCYVISPRLAHRVVGYLEEEAIHSYTEFLKDIDNGKIE 302

Query: 292 NVPAPAIAIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGY 350
           NV APAIAIDYWRLPKDA LKDV+TVIRADEAHHRDVNHFASDI  QGKELRE+AAP+GY
Sbjct: 303 NVAAPAIAIDYWRLPKDATLKDVVTVIRADEAHHRDVNHFASDIRNQGKELREAAAPIGY 353

BLAST of Lsi03G009480 vs. TAIR 10
Match: AT3G22370.1 (alternative oxidase 1A )

HSP 1 Score: 430.3 bits (1105), Expect = 1.5e-120
Identity = 210/318 (66.04%), Postives = 250/318 (78.62%), Query Frame = 0

Query: 49  QPMVDGGFQWRR------LLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKE--------- 108
           +P V   + W R      +  +ST    E+  +K E       NQ+K E E         
Sbjct: 42  KPGVTSAWIWTRAPTIGGMRFASTITLGEKTPMKEEDA-----NQKKTENESTGGDAAGG 101

Query: 109 --KENALVSSYWGIYRPKITREDGSEWPWNCFMPWETYRADVSIDLGKHHEPKTFLDKVA 168
             K +  ++SYWG+   KIT+EDGSEW WNCF PWETY+AD++IDL KHH P TFLD++A
Sbjct: 102 NNKGDKGIASYWGVEPNKITKEDGSEWKWNCFRPWETYKADITIDLKKHHVPTTFLDRIA 161

Query: 169 YRVVKLLRIPTDIFFQRRYGCRAVMLETVAAVPGMVGGMLLHLKSLRKFQHSGGWIKALL 228
           Y  VK LR PTD+FFQRRYGCRA+MLETVAAVPGMVGGMLLH KSLR+F+ SGGWIKALL
Sbjct: 162 YWTVKSLRWPTDLFFQRRYGCRAMMLETVAAVPGMVGGMLLHCKSLRRFEQSGGWIKALL 221

Query: 229 EEAENERMHLMTMIELVQPKWYERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEA 288
           EEAENERMHLMT +E+ +PKWYER LVITVQGVFFNA+F+ YL+SPK AHR+VGYLEEEA
Sbjct: 222 EEAENERMHLMTFMEVAKPKWYERALVITVQGVFFNAYFLGYLISPKFAHRMVGYLEEEA 281

Query: 289 IHSYTEYLKDIDEGKIENVPAPAIAIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASD 348
           IHSYTE+LK++D+G IENVPAPAIAIDYWRLP DA L+DV+ V+RADEAHHRDVNHFASD
Sbjct: 282 IHSYTEFLKELDKGNIENVPAPAIAIDYWRLPADATLRDVVMVVRADEAHHRDVNHFASD 341

Query: 349 IHFQGKELRESAAPLGYH 350
           IH+QG+EL+E+ AP+GYH
Sbjct: 342 IHYQGRELKEAPAPIGYH 354

BLAST of Lsi03G009480 vs. TAIR 10
Match: AT3G22360.1 (alternative oxidase 1B )

HSP 1 Score: 412.5 bits (1059), Expect = 3.3e-115
Identity = 187/256 (73.05%), Postives = 222/256 (86.72%), Query Frame = 0

Query: 94  KENALVSSYWGIYRPKITREDGSEWPWNCFMPWETYRADVSIDLGKHHEPKTFLDKVAYR 153
           K   L+ SYWG+   KIT+EDG+EW W+CF PWETY++D++IDL KHH P T  DK+AY 
Sbjct: 70  KGEQLIVSYWGVKPMKITKEDGTEWKWSCFRPWETYKSDLTIDLKKHHVPSTLPDKLAYW 129

Query: 154 VVKLLRIPTDIFFQRRYGCRAVMLETVAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEE 213
            VK LR PTD+FFQRRYGCRA+MLETVAAVPGMVGGML+H KSLR+F+ SGGWIKALLEE
Sbjct: 130 TVKSLRWPTDLFFQRRYGCRAMMLETVAAVPGMVGGMLVHCKSLRRFEQSGGWIKALLEE 189

Query: 214 AENERMHLMTMIELVQPKWYERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIH 273
           AENERMHLMT +E+ +P WYER LVI VQG+FFNA+F+ YL+SPK AHR+VGYLEEEAIH
Sbjct: 190 AENERMHLMTFMEVAKPNWYERALVIAVQGIFFNAYFLGYLISPKFAHRMVGYLEEEAIH 249

Query: 274 SYTEYLKDIDEGKIENVPAPAIAIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIH 333
           SYTE+LK++D G IENVPAPAIAIDYWRL  DA L+DV+ V+RADEAHHRDVNH+ASDIH
Sbjct: 250 SYTEFLKELDNGNIENVPAPAIAIDYWRLEADATLRDVVMVVRADEAHHRDVNHYASDIH 309

Query: 334 FQGKELRESAAPLGYH 350
           +QG+EL+E+ AP+GYH
Sbjct: 310 YQGRELKEAPAPIGYH 325

BLAST of Lsi03G009480 vs. TAIR 10
Match: AT3G27620.1 (alternative oxidase 1C )

HSP 1 Score: 412.1 bits (1058), Expect = 4.3e-115
Identity = 193/280 (68.93%), Postives = 233/280 (83.21%), Query Frame = 0

Query: 70  EEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWPWNCFMPWETY 129
           EE+G      G+K  +   K E+     L+ SYWG+   KIT+EDG+EW W+CF PWETY
Sbjct: 58  EEEGS---GDGVKVNDQGNKGEQ-----LIVSYWGVKPMKITKEDGTEWKWSCFRPWETY 117

Query: 130 RADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETVAAVPGMVGG 189
           +AD++IDL KHH P T  DK+AY +VK LR PTD+FFQRRYGCRA+MLETVAAVPGMVGG
Sbjct: 118 KADLTIDLKKHHVPSTLPDKIAYWMVKSLRWPTDLFFQRRYGCRAIMLETVAAVPGMVGG 177

Query: 190 MLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVITVQGVFFNAF 249
           ML+H KSLR+F+ SGGWIKALLEEAENERMHLMT +E+ +PKWYER LVI+VQGVFFNA+
Sbjct: 178 MLMHFKSLRRFEQSGGWIKALLEEAENERMHLMTFMEVAKPKWYERALVISVQGVFFNAY 237

Query: 250 FVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDYWRLPKDARLK 309
            + Y++SPK AHR+VGYLEEEAIHSYTE+LK++D G IENVPAPAIA+DYWRL  DA L+
Sbjct: 238 LIGYIISPKFAHRMVGYLEEEAIHSYTEFLKELDNGNIENVPAPAIAVDYWRLEADATLR 297

Query: 310 DVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           DV+ V+RADEAHHRDVNH+ASDIH+QG EL+E+ AP+GYH
Sbjct: 298 DVVMVVRADEAHHRDVNHYASDIHYQGHELKEAPAPIGYH 329

BLAST of Lsi03G009480 vs. TAIR 10
Match: AT1G32350.1 (alternative oxidase 1D )

HSP 1 Score: 405.2 bits (1040), Expect = 5.3e-113
Identity = 191/290 (65.86%), Postives = 229/290 (78.97%), Query Frame = 0

Query: 60  RLLSSSTAVAEEQGKVKLEQGIKDIENQEKKEKEKENALVSSYWGIYRPKITREDGSEWP 119
           RLLSS T+             +      E   +  +  ++S+YWGI   KIT+ DGS W 
Sbjct: 40  RLLSSDTS-----------SPVSGNNQPENPIRTADGKVISTYWGIPPTKITKPDGSAWK 99

Query: 120 WNCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLET 179
           WNCF PW++Y+ DVSID+ KHH+P  F DK AY  V+ L+IP  +FFQR++ C A++LET
Sbjct: 100 WNCFQPWDSYKPDVSIDVTKHHKPSNFTDKFAYWTVQTLKIPVQLFFQRKHMCHAMLLET 159

Query: 180 VAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVI 239
           VAAVPGMVGGMLLHLKSLR+F+HSGGWIKALLEEAENERMHLMT IEL QPKWYER +V 
Sbjct: 160 VAAVPGMVGGMLLHLKSLRRFEHSGGWIKALLEEAENERMHLMTFIELSQPKWYERAIVF 219

Query: 240 TVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAIAIDY 299
           TVQGVFFNA+F+ Y++SPKLAHRI GYLEEEA++SYTE+LKDID GK EN PAPAIAIDY
Sbjct: 220 TVQGVFFNAYFLAYVISPKLAHRITGYLEEEAVNSYTEFLKDIDAGKFENSPAPAIAIDY 279

Query: 300 WRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 350
           WRLPKDA L+DV+ VIRADEAHHRD+NH+ASDI F+G EL+E+ AP+GYH
Sbjct: 280 WRLPKDATLRDVVYVIRADEAHHRDINHYASDIQFKGHELKEAPAPIGYH 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q402944.0e-13471.08Ubiquinol oxidase, mitochondrial OS=Mangifera indica OX=29780 GN=AOMI 1 PE=1 SV=... [more]
Q412666.9e-13477.21Ubiquinol oxidase 2, mitochondrial OS=Glycine max OX=3847 GN=AOX2 PE=1 SV=2[more]
O220491.8e-12673.42Ubiquinol oxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX2 PE=1 ... [more]
O033767.1e-12362.39Alternative oxidase 3, mitochondrial OS=Glycine max OX=3847 GN=AOX3 PE=1 SV=1[more]
O828074.3e-12065.44Ubiquinol oxidase 1a, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Match NameE-valueIdentityDescription
G3G3W93.9e-19698.85Ubiquinol oxidase OS=Citrullus lanatus OX=3654 PE=3 SV=1[more]
D4P4J88.6e-19698.28Ubiquinol oxidase OS=Citrullus lanatus OX=3654 PE=2 SV=1[more]
A0A6J1JCS22.3e-18894.84Ubiquinol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111483245 PE=3 SV=1[more]
A0A6J1E6M01.8e-18593.41Ubiquinol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111430458 PE=3 SV=1[more]
A0A0A7DJW52.6e-18493.12Ubiquinol oxidase OS=Cucumis melo OX=3656 GN=LOC103503097 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AEN99850.18.0e-19698.85mitochondrial AOX2 [Citrullus lanatus][more]
ADD84880.11.8e-19598.28mitochondrial AOX2 [Citrullus lanatus][more]
XP_038876701.13.2e-18995.43ubiquinol oxidase, mitochondrial [Benincasa hispida][more]
XP_022985158.14.7e-18894.84ubiquinol oxidase, mitochondrial-like [Cucurbita maxima][more]
XP_023552001.18.8e-18793.98ubiquinol oxidase, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G64210.11.3e-12773.42alternative oxidase 2 [more]
AT3G22370.11.5e-12066.04alternative oxidase 1A [more]
AT3G22360.13.3e-11573.05alternative oxidase 1B [more]
AT3G27620.14.3e-11568.93alternative oxidase 1C [more]
AT1G32350.15.3e-11365.86alternative oxidase 1D [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 74..97
NoneNo IPR availablePIRSRPIRSR005229-1PIRSR005229-1coord: 168..333
e-value: 8.5E-71
score: 235.8
NoneNo IPR availablePANTHERPTHR31803:SF20ALTERNATIVE OXIDASE 3, MITOCHONDRIALcoord: 1..349
IPR002680Alternative oxidasePFAMPF01786AOXcoord: 139..329
e-value: 5.7E-81
score: 271.2
IPR002680Alternative oxidasePANTHERPTHR31803ALTERNATIVE OXIDASEcoord: 1..349
IPR002680Alternative oxidaseCDDcd01053AOXcoord: 166..333
e-value: 3.27726E-103
score: 298.737
IPR038659Alternative oxidase superfamilyGENE3D1.20.1260.140Alternative oxidasecoord: 111..346
e-value: 6.5E-97
score: 325.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G009480.1Lsi03G009480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0070469 respirasome
molecular_function GO:0009916 alternative oxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0102721 ubiquinol:oxygen oxidoreductase activity