Lsi03G009010 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G009010
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPectin lyase-like superfamily protein
Locationchr03: 16472791 .. 16475478 (+)
RNA-Seq ExpressionLsi03G009010
SyntenyLsi03G009010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTCTTCATACAAATCTCTCCAAATTCTAAAACACTTTATTTGCAAGAAATGACTCTTCATACAAATTCATTTTTTCTCTTGCTACTACTACTACCCATTATGACGTTCTTCAATTTACCTACCTTTGCTGCTCCGTTAACATTCAACGTTGTCAATTTTGGAGCCAAACCCAACAACCCCAAAATCGACTCATCGAAGGCCTTCCAATTGGCATGGATGCGTGCTTGTACCTCTTCTACACCAACATCCATTTATGTGCCTAACGCCAAATTCTACGTTCATAGTGCAACTTTTAATGGGCCATGCAATAATAATGACATTACTATACGAATTGATGGTACTCTCGTGGCTCCCTCAGACTTCCATCTCACTGGCAACTCTGAAAATTGGATCATTTTTCACAATGTTAATGGAGTTTCTGTCTTGGGTGGCATTATTGATGGCCAAGGATCTGGGCTTTGGGCTTGCAAAATCTCTGGCAAGACCTGTCCTCATGGAGCTACGGTATACAAACTTTACCAACTTTATGTCTATTAATATTTTGTTAGCATCTAGGTTGAACTTATATTTCTTCCATGCACAACAACAAAAAGAATATTTTAATTGAGTGCATCTCGAGATGCATCCATATTTGTGCAACCCATCCAATAGCCTAATCACAATTTGTGATGTGACAAACTTTTTTTCTTTTTTTTTTTTTATAAATATTTTTGAACTTTTTTTATATCATTAGCGAGAGCTACAGGAAGATGGGTGCAACTCAGTATTACGCATATTTAAACTATAAGAAATAAGAGTTTATCATGTAATAAGCTCTATTTAGACAACTAAATAGGTGCACTCACGTACACTAAATTAATTTATCTTTTGAAAATTAAATATGTATTAAATAACATGATTGTATTTTTTTTTAAAAAAAAAAAAAGAAAAGAATTAAACAGAAAATAAAGGATACTATTTTAAAAAATTAAAATTATTATACGTTATTTATTATTGTCATGCTGGCATACTTTCCTTCCCTTTACACTGAATTCACTCTCTAATATAAAAAGAACTGTTGTGTTTTGTGCAGTCACTAAAATTTACAAATTCCCAAAACATATTGGTTAGTGGATTAACTTCGCTAAATAGCCAAATGTTTCACATAGTGGTAAACCAATGTCATAATGTAAAAATGCAAGGACTAAAGGTCTCGGCCCTTGGCAATAGCCCAAACACCGATGGAATTCACGTAGGTGGATCATCTCATGTCACCATCCTTAACTCCAATATCGGCACCGGTGACGACTGCGTCTCAATAGGCCCTGGCACTTCCAACTTATGGATTGAGAATATTGTTTGTGGACCTGGCCATGGAATTAGGTACGTAGTCACTTGTTAAATTTTTATCAGTTAGTTGAGATTTTTTGTAGATAATTTTGATTTGATTGGTGCAGCATTGGGAGTTTAGGAAAAGGGCCGAAGGAGGACGGCGTTGAGAACGTGACAGTAAAATCATGTAGGTTTATGAACACTCAAAATGGGGTGAGAATAAAAAGTTGGGGAAGGTCAAGTAGTGGGTTTGCAAGAAATATACGTTTTCAACATATTACAATGACTAATGTCCAAAATCCTATTATCATCGATCAAAATTATTGTCCCCACAATCAAGGTTGCCCAGGACAGGTGCGTATCGTACGATTGTTAATCATATTGATCTTTTTTTATTGTTATTATTATCTTCGACTCATTACGCCTCTTTTGAAAAAAAATCATTTGCTGAGACGAAAATAAAGATATTAAATTCCTATCAAGATAACTAAAAGTTACAACTATTAAAAATCTGAATGAACCTAAAAAAAGATAAAGTCCAACATAAATTGCTTATTTTTCGAGATTCATTTGTATATATATCTATTAATATATACAAACAAAGGGGTATATATAAGTCTATAATTTTATGTCAAATGAGGTTTTGATTCAATTAAATACACAATGTTAACTTCATTCATTAATTTGAAATATATATCGATTTTTAAAACTCTATCTTTCCATTATGTTTAGTATATTAGGTTTTGAGTAAAAAAAAAATATGACTTTAGTATTTGTTATTGATAAGAGATGAAAGATGTTCTAGGGTTAAAACTCGAATTTTGGTCCTTTTTAACAAAGTAAAAATTTAGTCTTTAAACATTTAAGGAACTTCAATTTGTAATGATTTATTCCTTTTACTTTTATAAAGCTTTTTCAGAAAAAAAAAATCAATAACTAAAATTTAATTAAATTGACTTGCAATCTTTTTTTTAAATCAGACAAATATTTAATTCCTCGTTCATATAATTGTTGTACTAAAATTTAATTAAATTCGGTTGTTTGTGAATGAATAGGGTTCTGGAATTAAAGTTAGCGATGTGACATATGAAGACATTCACGAGACATCGAACACGCAAGTGGCCATGAAATTCGATTGTAGCCCAAAATTTCCATGCAAAGGAATAAGGTTGGAGGATATTAAGCTTACTTACAAGAATCAAGTAGCCAAAGCTTCATGTAGTAATGCTCAAGGATGTGATGTCGGCTTGGTTCAGCCTATGGGTTGCTTTTAGGGAAATTTAATTCTTAAGCTTTATATAATTTATTATTTTAATTTAGTGTTATTCTTAAGCATATTGTGAAGGAAGTTAGATGAATAAATGGTTTGTTATT

mRNA sequence

CTTTTCTTCATACAAATCTCTCCAAATTCTAAAACACTTTATTTGCAAGAAATGACTCTTCATACAAATTCATTTTTTCTCTTGCTACTACTACTACCCATTATGACGTTCTTCAATTTACCTACCTTTGCTGCTCCGTTAACATTCAACGTTGTCAATTTTGGAGCCAAACCCAACAACCCCAAAATCGACTCATCGAAGGCCTTCCAATTGGCATGGATGCGTGCTTGTACCTCTTCTACACCAACATCCATTTATGTGCCTAACGCCAAATTCTACGTTCATAGTGCAACTTTTAATGGGCCATGCAATAATAATGACATTACTATACGAATTGATGGTACTCTCGTGGCTCCCTCAGACTTCCATCTCACTGGCAACTCTGAAAATTGGATCATTTTTCACAATGTTAATGGAGTTTCTGTCTTGGGTGGCATTATTGATGGCCAAGGATCTGGGCTTTGGGCTTGCAAAATCTCTGGCAAGACCTGTCCTCATGGAGCTACGTCACTAAAATTTACAAATTCCCAAAACATATTGGTTAGTGGATTAACTTCGCTAAATAGCCAAATGTTTCACATAGTGGTAAACCAATGTCATAATGTAAAAATGCAAGGACTAAAGGTCTCGGCCCTTGGCAATAGCCCAAACACCGATGGAATTCACGTAGGTGGATCATCTCATGTCACCATCCTTAACTCCAATATCGGCACCGGTGACGACTGCGTCTCAATAGGCCCTGGCACTTCCAACTTATGGATTGAGAATATTGTTTGTGGACCTGGCCATGGAATTAGCATTGGGAGTTTAGGAAAAGGGCCGAAGGAGGACGGCGTTGAGAACGTGACAGTAAAATCATGTAGGTTTATGAACACTCAAAATGGGGGTTCTGGAATTAAAGTTAGCGATGTGACATATGAAGACATTCACGAGACATCGAACACGCAAGTGGCCATGAAATTCGATTGTAGCCCAAAATTTCCATGCAAAGGAATAAGGTTGGAGGATATTAAGCTTACTTACAAGAATCAAGTAGCCAAAGCTTCATGTAGTAATGCTCAAGGATGTGATGTCGGCTTGGTTCAGCCTATGGGTTGCTTTTAGGGAAATTTAATTCTTAAGCTTTATATAATTTATTATTTTAATTTAGTGTTATTCTTAAGCATATTGTGAAGGAAGTTAGATGAATAAATGGTTTGTTATT

Coding sequence (CDS)

ATGACTCTTCATACAAATTCATTTTTTCTCTTGCTACTACTACTACCCATTATGACGTTCTTCAATTTACCTACCTTTGCTGCTCCGTTAACATTCAACGTTGTCAATTTTGGAGCCAAACCCAACAACCCCAAAATCGACTCATCGAAGGCCTTCCAATTGGCATGGATGCGTGCTTGTACCTCTTCTACACCAACATCCATTTATGTGCCTAACGCCAAATTCTACGTTCATAGTGCAACTTTTAATGGGCCATGCAATAATAATGACATTACTATACGAATTGATGGTACTCTCGTGGCTCCCTCAGACTTCCATCTCACTGGCAACTCTGAAAATTGGATCATTTTTCACAATGTTAATGGAGTTTCTGTCTTGGGTGGCATTATTGATGGCCAAGGATCTGGGCTTTGGGCTTGCAAAATCTCTGGCAAGACCTGTCCTCATGGAGCTACGTCACTAAAATTTACAAATTCCCAAAACATATTGGTTAGTGGATTAACTTCGCTAAATAGCCAAATGTTTCACATAGTGGTAAACCAATGTCATAATGTAAAAATGCAAGGACTAAAGGTCTCGGCCCTTGGCAATAGCCCAAACACCGATGGAATTCACGTAGGTGGATCATCTCATGTCACCATCCTTAACTCCAATATCGGCACCGGTGACGACTGCGTCTCAATAGGCCCTGGCACTTCCAACTTATGGATTGAGAATATTGTTTGTGGACCTGGCCATGGAATTAGCATTGGGAGTTTAGGAAAAGGGCCGAAGGAGGACGGCGTTGAGAACGTGACAGTAAAATCATGTAGGTTTATGAACACTCAAAATGGGGGTTCTGGAATTAAAGTTAGCGATGTGACATATGAAGACATTCACGAGACATCGAACACGCAAGTGGCCATGAAATTCGATTGTAGCCCAAAATTTCCATGCAAAGGAATAAGGTTGGAGGATATTAAGCTTACTTACAAGAATCAAGTAGCCAAAGCTTCATGTAGTAATGCTCAAGGATGTGATGTCGGCTTGGTTCAGCCTATGGGTTGCTTTTAG

Protein sequence

MTLHTNSFFLLLLLLPIMTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRACTSSTPTSIYVPNAKFYVHSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHNVNGVSVLGGIIDGQGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVVNQCHNVKMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIENIVCGPGHGISIGSLGKGPKEDGVENVTVKSCRFMNTQNGGSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCKGIRLEDIKLTYKNQVAKASCSNAQGCDVGLVQPMGCF
Homology
BLAST of Lsi03G009010 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 3.2e-115
Identity = 216/387 (55.81%), Postives = 259/387 (66.93%), Query Frame = 0

Query: 11  LLLLLPIMTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRACTSSTPTSIYV 70
           L  L  I  F      A+PLT+NV + GAK +  K DS+KAF  AW +AC S  P  IYV
Sbjct: 7   LFSLSLIFVFMINSAIASPLTYNVASLGAKADG-KTDSTKAFLSAWAKACASMNPGVIYV 66

Query: 71  PNAKFYVHSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHNVNGVSVLGGII 130
           P   F++    F+GPC NN IT RI GTLVAPSD+ + GN+ NWI FH+VNGV++ GGI+
Sbjct: 67  PAGTFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGIL 126

Query: 131 DGQGSGLWACK-ISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVVNQCHNVKMQG 190
           DGQG+ LWACK   G++CP GAT+L F++S NI+VSGL SLNSQMFHIV+N   NV+MQG
Sbjct: 127 DGQGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQG 186

Query: 191 LKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIENIVCGPGHGIS 250
           ++VS  GNSPNTDGIHV  SS VTILNS I TGDDCVSIGPGTSNLWIE + CGPGHGIS
Sbjct: 187 VRVSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGPGHGIS 246

Query: 251 IGSLGKGPKEDGVENVTVKSCRFMNTQNG------------------------------- 310
           IGSLGK  +E GV+NVTVK+  F  TQNG                               
Sbjct: 247 IGSLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVNVENPI 306

Query: 311 ----------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCKGIRLEDIKL 350
                            SG+++SDVTYEDIH TS T+VA+KFDCSPK PC+ I+LED+KL
Sbjct: 307 VIDQHYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVKL 366

BLAST of Lsi03G009010 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 3.4e-88
Identity = 181/386 (46.89%), Postives = 239/386 (61.92%), Query Frame = 0

Query: 3   LHTNSFFLLLLLLPIMTFFNLPTFAAPL--TFNVVNFGAKPNNPKIDSSKAFQLAWMRAC 62
           ++  S  +L L L I+ F  + + A P+  T NV+++GAKP+  K DS+KAF  AW  AC
Sbjct: 2   VYNTSCLILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSK-DSTKAFLAAWDVAC 61

Query: 63  TSSTPTSIYVPNAKFYVHSATFNG-PCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHN 122
            S+ PT+I VP  +F V +  F+G  C    I+IRI G++VAP DF +  +S++WI F +
Sbjct: 62  ASANPTTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFED 121

Query: 123 VNGVSVLGGIIDGQGSGLWACKIS-GKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIV 182
           V  VS+ GGI+D QG+ LW CK + G  CP GA SL F+ S NI +SGLTS+NSQ FHIV
Sbjct: 122 VTDVSIYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIV 181

Query: 183 VNQCHNVKMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIE 242
           ++  +NV + G+KVSA  NSPNTDGIHV  S  V I NS IGTGDDC+SIGPG++N++I+
Sbjct: 182 IDNSNNVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQ 241

Query: 243 NIVCGPGHGISIGSLGKGPKEDGVENVTVKSCRFMNTQNG-------------------- 302
            I CGPGHGISIGSLG+  +E GV+NVTV +  FM T NG                    
Sbjct: 242 TIRCGPGHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQ 301

Query: 303 --------------------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPC 339
                                      SG+KVS+V YEDIH TSNT+VA+  DCS + PC
Sbjct: 302 HINMKMVKNPIIIDQHYCLHKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPC 361

BLAST of Lsi03G009010 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.6e-59
Identity = 136/373 (36.46%), Postives = 189/373 (50.67%), Query Frame = 0

Query: 31  TFNVVNFGAKPNNPKIDSSKAFQLAWMRACTSSTPTSIYVPNAKFYVHSATFNGPCNNND 90
           +F++   GA   N K DS+KA Q AW  AC  +   +I +P   F V    F GPC   D
Sbjct: 40  SFDITKLGAS-GNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPC-KGD 99

Query: 91  ITIRIDGTLVAPSDFHLTGNSENWIIFHNVNGVSVLG-GIIDGQGSGLWACKISGK--TC 150
           +TI++DG L+A +D        NWI    V+ + + G G +DGQG  +W+     K   C
Sbjct: 100 VTIQVDGNLLATTDLSQYKEHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDC 159

Query: 151 PHGATSLKFTNSQNILVSGLTSLNSQMFHIVVNQCHNVKMQGLKVSALGNSPNTDGIHVG 210
                SL      N  VSG+T LNS+ FH+ + QC N+ ++ + V+A G+SPNTDGIH+G
Sbjct: 160 KILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIKDVTVTAPGDSPNTDGIHMG 219

Query: 211 GSSHVTILNSNIGTGDDCVSIGPGTSNLWIENIVCGPGHGISIGSLGKGPKEDGVENVTV 270
            SS +TI N+ IG GDDC+SIGPGTS + I  + CGPGHGISIGSLG+   E  V ++ V
Sbjct: 220 DSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINV 279

Query: 271 KSCRFMNTQ------------------------------------------------NGG 330
           K C    T                                                 NG 
Sbjct: 280 KDCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGA 339

Query: 331 SGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCKGIRLEDIKLTYK--NQVAKASCSNAQ 351
           S + V DVT+++I  TS+T  A+   C+ K PC G  ++D+ + Y   N    A C+NA+
Sbjct: 340 SKVTVKDVTFKNITGTSSTPEAISLLCTAKVPCTGATMDDVNVEYSGTNNKTMAICTNAK 399

BLAST of Lsi03G009010 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.6e-58
Identity = 134/373 (35.92%), Postives = 191/373 (51.21%), Query Frame = 0

Query: 31  TFNVVNFGAKPNNPKIDSSKAFQLAWMRACTSSTPTSIYVPNAKFYVHSATFNGPCNNND 90
           +F++   GA   N K DS+KA Q AW  AC  +   +I +P   F V    F GPC   D
Sbjct: 40  SFDITKLGAS-GNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPC-KGD 99

Query: 91  ITIRIDGTLVAPSDFHLTGNSENWIIFHNVNGVSVLG-GIIDGQGSGLWACKISGK--TC 150
           +TI++DG L+A +D     +  NWI    V+ + + G G +DGQG  +W+     K   C
Sbjct: 100 VTIQVDGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDC 159

Query: 151 PHGATSLKFTNSQNILVSGLTSLNSQMFHIVVNQCHNVKMQGLKVSALGNSPNTDGIHVG 210
                SL      N  VSG+T LNS+ FH+ + +C ++ ++ + V+A G+SPNTDGIH+G
Sbjct: 160 KILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMG 219

Query: 211 GSSHVTILNSNIGTGDDCVSIGPGTSNLWIENIVCGPGHGISIGSLGKGPKEDGVENVTV 270
            SS +TI N+ IG GDDC+SIGPGTS + I  + CGPGHGISIGSLG+   E  V ++ V
Sbjct: 220 DSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINV 279

Query: 271 KSCRFMNTQ------------------------------------------------NGG 330
           K C    T                                                 NG 
Sbjct: 280 KDCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGA 339

Query: 331 SGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCKGIRLEDIKLTYK--NQVAKASCSNAQ 351
           S + V DVT+++I  TS+T  A+   C+ K PC G+ ++D+ + Y   N    A C+NA+
Sbjct: 340 SKVTVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNAK 399

BLAST of Lsi03G009010 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 6.2e-58
Identity = 131/358 (36.59%), Postives = 187/358 (52.23%), Query Frame = 0

Query: 32  FNVVNFGAKPNNPKIDSSKAFQLAWMRACTSSTPTSIYVPNAKFYVHSATFNGPCNNNDI 91
           FNV ++GAK      D S+A   AW  AC S  P+++ +P   + +      GPC  + I
Sbjct: 10  FNVNDYGAKGAG---DISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKI 69

Query: 92  TIRIDGTLVAPSDFHLTGNSENWIIFHNVNGVSVLG-GIIDGQGSGLWACKISGK--TCP 151
             +IDG + AP+D      S+ W+ F+ ++G++V G G +DGQG   WA     K   C 
Sbjct: 70  GFQIDGVVKAPAD-PSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCK 129

Query: 152 HGATSLKFTNSQNILVSGLTSLNSQMFHIVVNQCHNVKMQGLKVSALGNSPNTDGIHVGG 211
           H A +L+F   ++ +V  +TSLNS+MFHI V +C ++  Q + V+A G S NTDGIHVG 
Sbjct: 130 HAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGI 189

Query: 212 SSHVTILNSNIGTGDDCVSIGPGTSNLWIENIVCGPGHGISIGSLGKGPKEDGVENVTVK 271
           S  VTI N+ I TGDDC+SIGPG+ N+ I  + CGPGHGISIGSLG+   E  V  +TVK
Sbjct: 190 SKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVK 249

Query: 272 SCRFMNTQNG-----------------------------------------------GSG 331
            C F  T NG                                                S 
Sbjct: 250 GCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSR 309

Query: 332 IKVSDVTYEDIHETSNTQVAMKFDCSPKFPCKGIRLEDIKLTYKNQ--VAKASCSNAQ 338
           IK+S++ + +I  TS  +VA+   CS   PC  +++ +I L+Y+     A ++CSN +
Sbjct: 310 IKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSNVK 363

BLAST of Lsi03G009010 vs. ExPASy TrEMBL
Match: A0A0A0K6Z5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G145950 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.2e-151
Identity = 277/398 (69.60%), Postives = 304/398 (76.38%), Query Frame = 0

Query: 1   MTLHTNSFFLLLLLLPI-MTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRA 60
           M LHT + FL  L L + M F NL  FA PLTFNV++FG KPNN + DSSKA QLAWM A
Sbjct: 3   MALHTIASFLFFLTLTVLMLFNNLAAFATPLTFNVLDFGTKPNNAETDSSKALQLAWMHA 62

Query: 61  CTSSTPTSIYVPNAKFYVHSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHN 120
           C+SS P +IYVP AKFYV SATFNGPC NNDITIRIDGTLVAPSDF LT NSENWI+FHN
Sbjct: 63  CSSSKPATIYVPKAKFYVRSATFNGPCKNNDITIRIDGTLVAPSDFRLTSNSENWILFHN 122

Query: 121 VNGVSVLGGIIDGQGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVV 180
           VNGV+V+GGIIDGQG+ LWACK S  TCP G TSL+FTNSQNILV+GLTSLNSQ+FHIVV
Sbjct: 123 VNGVTVIGGIIDGQGTQLWACKNSANTCPSGTTSLEFTNSQNILVNGLTSLNSQIFHIVV 182

Query: 181 NQCHNVKMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIEN 240
           NQC NVKMQGLKV A  NSPNTDGIHVG SSHVTILNSNI TGDDC+SIGPG+SNLWIEN
Sbjct: 183 NQCRNVKMQGLKVFAASNSPNTDGIHVGDSSHVTILNSNIRTGDDCISIGPGSSNLWIEN 242

Query: 241 IVCGPGHGISIGSLGKGPKEDGVENVTVKSCRFMNTQNG--------------------- 300
           +VCGPGHGISIGSLGK  KE GV+NVTVKSC+FM+TQNG                     
Sbjct: 243 VVCGPGHGISIGSLGKELKEKGVQNVTVKSCKFMDTQNGVRIKSWGRQSSGFAKDIRFQH 302

Query: 301 --------------------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPC 351
                                      SG+ VSDV Y++IH TS T VA+KFDCSPKFPC
Sbjct: 303 LTMTNVQNPIIIDQNYCPHNQGCPGQSSGVTVSDVKYKNIHGTSATPVAIKFDCSPKFPC 362

BLAST of Lsi03G009010 vs. ExPASy TrEMBL
Match: A0A5A7U863 (Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G00270 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 6.7e-148
Identity = 264/380 (69.47%), Postives = 291/380 (76.58%), Query Frame = 0

Query: 18  MTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRACTSSTPTSIYVPNAKFYV 77
           M F NL  F  PLTFNVV+FG KPNN + DSSKA QLAWM AC+SS P +IYVP AKFYV
Sbjct: 1   MLFNNLAAFPTPLTFNVVDFGTKPNNAETDSSKALQLAWMHACSSSKPATIYVPKAKFYV 60

Query: 78  HSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHNVNGVSVLGGIIDGQGSGL 137
            SATFNGPC NNDITIRIDG L+APSDFHLT NSENWI+FHNVNGV+VLGGI+DGQG+ L
Sbjct: 61  RSATFNGPCKNNDITIRIDGALMAPSDFHLTSNSENWILFHNVNGVTVLGGILDGQGTQL 120

Query: 138 WACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVVNQCHNVKMQGLKVSALGN 197
           WACK S  TCP G TSL+FTNSQNILV+GLT+LNSQ++HIVVNQC NVKMQGLKVSA GN
Sbjct: 121 WACKNSANTCPRGTTSLEFTNSQNILVNGLTALNSQLYHIVVNQCRNVKMQGLKVSAAGN 180

Query: 198 SPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIENIVCGPGHGISIGSLGKGP 257
           SPNTDGIHVG SSHVTILN+NI TGDDC+SIGPG+SNLWIENI CGPGHGISIGSL K  
Sbjct: 181 SPNTDGIHVGDSSHVTILNTNIRTGDDCISIGPGSSNLWIENIACGPGHGISIGSLAKDF 240

Query: 258 KEDGVENVTVKSCRFMNTQNG--------------------------------------- 317
           KE GV+NVTVKSC+FM+TQNG                                       
Sbjct: 241 KEKGVQNVTVKSCKFMDTQNGVRIKSWGRQSNGFARNIRFQHLTMTNVQNPIIIDQNYCP 300

Query: 318 --------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCKGIRLEDIKLTYKNQVAK 351
                    SG+ VSDV Y DI  TS T VA+KFDCSPKFPCKGI+LED+KL+YKNQVAK
Sbjct: 301 HNQGCPGQSSGVAVSDVKYTDIRGTSATPVAIKFDCSPKFPCKGIKLEDVKLSYKNQVAK 360

BLAST of Lsi03G009010 vs. ExPASy TrEMBL
Match: A0A1S3BJI1 (polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103490378 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 8.7e-148
Identity = 270/398 (67.84%), Postives = 298/398 (74.87%), Query Frame = 0

Query: 1   MTLHT-NSFFLLLLLLPIMTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRA 60
           M LH   SF L   L  +M F NL  F  PLTFNVV+FG KPNN + DSSKA QLAWM A
Sbjct: 1   MALHAITSFLLFSTLTVLMLFNNLAAFPTPLTFNVVDFGTKPNNAETDSSKALQLAWMHA 60

Query: 61  CTSSTPTSIYVPNAKFYVHSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHN 120
           C+SS P +IYVP AKFYV SATFNGPC NN ITIRIDG L+APSDFHLT NSENWI+FHN
Sbjct: 61  CSSSKPATIYVPKAKFYVRSATFNGPCKNNHITIRIDGALMAPSDFHLTSNSENWILFHN 120

Query: 121 VNGVSVLGGIIDGQGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVV 180
           VNGV+VLGGI+DGQG+ LWACK S  TCP G TSL+FTNSQNILV+GLT+LNSQ++HIVV
Sbjct: 121 VNGVTVLGGILDGQGTQLWACKNSANTCPRGTTSLEFTNSQNILVNGLTALNSQLYHIVV 180

Query: 181 NQCHNVKMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIEN 240
           NQC NVKMQGLKVSA GNSPNTDGIHVG SSHVTILN+NI TGDDC+SIGPG+SNLWIEN
Sbjct: 181 NQCRNVKMQGLKVSAAGNSPNTDGIHVGDSSHVTILNTNIRTGDDCISIGPGSSNLWIEN 240

Query: 241 IVCGPGHGISIGSLGKGPKEDGVENVTVKSCRFMNTQNG--------------------- 300
           I CGPGHGISIGSL K  KE GV+NVTVKSC+FM+TQNG                     
Sbjct: 241 IACGPGHGISIGSLAKDFKEKGVQNVTVKSCKFMDTQNGVRIKSWGRQSIGFARNIRFQH 300

Query: 301 --------------------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPC 351
                                      SG+ VSDV Y DI  TS T VA+KFDCSPKFPC
Sbjct: 301 LTMTNVQNPIIIDQNYCPHNQGCPGQSSGVAVSDVKYTDIRGTSATPVAIKFDCSPKFPC 360

BLAST of Lsi03G009010 vs. ExPASy TrEMBL
Match: A0A6J1GNK7 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111455565 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 6.5e-135
Identity = 255/389 (65.55%), Postives = 285/389 (73.26%), Query Frame = 0

Query: 1   MTLHTNSFFLLLLLLPIMTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRAC 60
           M   T SF L LLL+    FFN P FAA  TFNVV+FGAKP + K DSSKAFQ AW RAC
Sbjct: 24  MDPRTTSFLLSLLLI---IFFNSP-FAASETFNVVSFGAKP-DAKTDSSKAFQAAWARAC 83

Query: 61  TSSTPTSIYVPNAKFYVHSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHNV 120
           TSST  SIYVPN +FY+  ATFNGPC N+ I I IDGTLVAPSDFH+  NS NWIIFH V
Sbjct: 84  TSSTSASIYVPNGRFYLRHATFNGPCRNDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLV 143

Query: 121 NGVSVLGGIIDGQGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVVN 180
           NGVS+ GG +DGQG+GLWACK S KTCP GATSL+FT+S+NI++SGLTSLNSQ+FHIVVN
Sbjct: 144 NGVSIFGGTLDGQGAGLWACKRSAKTCPRGATSLEFTDSKNIMISGLTSLNSQIFHIVVN 203

Query: 181 QCHNVKMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIENI 240
           +CHNVKM+GLKVSA GNSPNTDGIHVG SSHVTILNS+I TGDDC+SIGPGTSNLWIEN+
Sbjct: 204 ECHNVKMEGLKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENV 263

Query: 241 VCGPGHGISIGSLGKGPKEDGVENVTVKSCRFMNTQNG---------------------- 300
            CGPGHGISIGSLGK  KEDGV+NVTVKSCRFMNTQNG                      
Sbjct: 264 ACGPGHGISIGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHL 323

Query: 301 -------------------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCK 343
                                     SGIKVSDVTY+DI  T+ T+ AM FDCSPKFPC 
Sbjct: 324 KMTNVKNPIIIDQNYCPYNKDCPGQASGIKVSDVTYQDIRGTAATRFAMTFDCSPKFPCV 383

BLAST of Lsi03G009010 vs. ExPASy TrEMBL
Match: A0A6J1I9T6 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470567 PE=3 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 4.2e-134
Identity = 254/389 (65.30%), Postives = 284/389 (73.01%), Query Frame = 0

Query: 1   MTLHTNSFFLLLLLLPIMTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRAC 60
           M   T SF   LL L ++ FFN P FAA  T NVVNFGAKP + K DSSKAFQ AW RAC
Sbjct: 1   MDPRTTSF---LLSLQLVIFFNSP-FAASETLNVVNFGAKP-DAKTDSSKAFQAAWARAC 60

Query: 61  TSSTPTSIYVPNAKFYVHSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHNV 120
           TSST +SIYVPN +FY+  ATFNGPC ++ I I IDGTLVAPSDFH+  NS NWIIFH V
Sbjct: 61  TSSTSSSIYVPNGRFYLRHATFNGPCRSDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLV 120

Query: 121 NGVSVLGGIIDGQGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVVN 180
           NGVS+ GG +DGQG+GLWACK S KTCP GATSL+FT+S+NIL+SGLTSLNSQ+FHIVVN
Sbjct: 121 NGVSIFGGTLDGQGAGLWACKRSAKTCPRGATSLEFTDSKNILISGLTSLNSQIFHIVVN 180

Query: 181 QCHNVKMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIENI 240
           +CHNVKM+GLKVSA GNSPNTDGIHVG SSHVTILNS+I TGDDC+SIGPGTSNLWIEN+
Sbjct: 181 ECHNVKMEGLKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENV 240

Query: 241 VCGPGHGISIGSLGKGPKEDGVENVTVKSCRFMNTQNG---------------------- 300
            CGPGHGISIGSLGK  KEDGV+NVTVKSCRF NTQNG                      
Sbjct: 241 ACGPGHGISIGSLGKALKEDGVQNVTVKSCRFTNTQNGVRIKSWGRPSSGFARNIRFQHL 300

Query: 301 -------------------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCK 343
                                     SGIKVSDVTY+DI  T+ T+ AM FDCSPKFPC 
Sbjct: 301 KMTNVKNPIIIDQNYCPYNKNCPGQASGIKVSDVTYQDIRGTAATRFAMTFDCSPKFPCV 360

BLAST of Lsi03G009010 vs. NCBI nr
Match: XP_038888717.1 (polygalacturonase-like [Benincasa hispida])

HSP 1 Score: 565.1 bits (1455), Expect = 4.3e-157
Identity = 290/397 (73.05%), Postives = 309/397 (77.83%), Query Frame = 0

Query: 1   MTLHTNSFFLLLLLLPIMTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRAC 60
           M  HTNSF LLLLLL IM+ FNL  FAAPLTFNVV+FGAKP+N K DSSKAF LAW RAC
Sbjct: 1   MAFHTNSFLLLLLLLVIMS-FNLAAFAAPLTFNVVDFGAKPDNAKTDSSKAFLLAWTRAC 60

Query: 61  TSSTPTSIYVPNAKFYVHSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHNV 120
           +SS PTSIYVP AKFYV SATFNGPC NN ITIRIDGTLVA SDFHL   SE+WIIFHNV
Sbjct: 61  SSSIPTSIYVPKAKFYVRSATFNGPCKNNAITIRIDGTLVASSDFHLNVKSEHWIIFHNV 120

Query: 121 NGVSVLGGIIDGQGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVVN 180
           NGV+VLGG+IDGQGSGLWACK SGK CP GATSLKFT+SQNILVSGL SLNSQ+FHIVVN
Sbjct: 121 NGVNVLGGVIDGQGSGLWACKNSGKVCPRGATSLKFTDSQNILVSGLASLNSQIFHIVVN 180

Query: 181 QCHNVKMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIENI 240
           QCHNVKM GLKVSA  NSPNTDGIH+GGSS+VTI NS IGTGDDC+SIGPGTSNLWIENI
Sbjct: 181 QCHNVKMHGLKVSASSNSPNTDGIHIGGSSYVTISNSKIGTGDDCISIGPGTSNLWIENI 240

Query: 241 VCGPGHGISIGSLGKGPKEDGVENVTVKSCRFMNTQNG---------------------- 300
            CGPGHGISIGSLGK  KE+GVENVTVKSC F+NTQNG                      
Sbjct: 241 ACGPGHGISIGSLGKELKENGVENVTVKSCGFINTQNGVRIKSWGRPSSGFARNIIFQHI 300

Query: 301 -------------------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCK 351
                                     SGIK+SDVTYEDIH TS T V +KFDCSPK+PCK
Sbjct: 301 TMTNVQNPIIIDQNYCPHNQGCPGQDSGIKISDVTYEDIHGTSATPVGIKFDCSPKYPCK 360

BLAST of Lsi03G009010 vs. NCBI nr
Match: XP_031745066.1 (polygalacturonase [Cucumis sativus])

HSP 1 Score: 545.0 bits (1403), Expect = 4.6e-151
Identity = 277/398 (69.60%), Postives = 304/398 (76.38%), Query Frame = 0

Query: 1   MTLHTNSFFLLLLLLPI-MTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRA 60
           M LHT + FL  L L + M F NL  FA PLTFNV++FG KPNN + DSSKA QLAWM A
Sbjct: 3   MALHTIASFLFFLTLTVLMLFNNLAAFATPLTFNVLDFGTKPNNAETDSSKALQLAWMHA 62

Query: 61  CTSSTPTSIYVPNAKFYVHSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHN 120
           C+SS P +IYVP AKFYV SATFNGPC NNDITIRIDGTLVAPSDF LT NSENWI+FHN
Sbjct: 63  CSSSKPATIYVPKAKFYVRSATFNGPCKNNDITIRIDGTLVAPSDFRLTSNSENWILFHN 122

Query: 121 VNGVSVLGGIIDGQGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVV 180
           VNGV+V+GGIIDGQG+ LWACK S  TCP G TSL+FTNSQNILV+GLTSLNSQ+FHIVV
Sbjct: 123 VNGVTVIGGIIDGQGTQLWACKNSANTCPSGTTSLEFTNSQNILVNGLTSLNSQIFHIVV 182

Query: 181 NQCHNVKMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIEN 240
           NQC NVKMQGLKV A  NSPNTDGIHVG SSHVTILNSNI TGDDC+SIGPG+SNLWIEN
Sbjct: 183 NQCRNVKMQGLKVFAASNSPNTDGIHVGDSSHVTILNSNIRTGDDCISIGPGSSNLWIEN 242

Query: 241 IVCGPGHGISIGSLGKGPKEDGVENVTVKSCRFMNTQNG--------------------- 300
           +VCGPGHGISIGSLGK  KE GV+NVTVKSC+FM+TQNG                     
Sbjct: 243 VVCGPGHGISIGSLGKELKEKGVQNVTVKSCKFMDTQNGVRIKSWGRQSSGFAKDIRFQH 302

Query: 301 --------------------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPC 351
                                      SG+ VSDV Y++IH TS T VA+KFDCSPKFPC
Sbjct: 303 LTMTNVQNPIIIDQNYCPHNQGCPGQSSGVTVSDVKYKNIHGTSATPVAIKFDCSPKFPC 362

BLAST of Lsi03G009010 vs. NCBI nr
Match: KAA0049805.1 (polygalacturonase-like [Cucumis melo var. makuwa] >TYK23060.1 polygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 533.5 bits (1373), Expect = 1.4e-147
Identity = 264/380 (69.47%), Postives = 291/380 (76.58%), Query Frame = 0

Query: 18  MTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRACTSSTPTSIYVPNAKFYV 77
           M F NL  F  PLTFNVV+FG KPNN + DSSKA QLAWM AC+SS P +IYVP AKFYV
Sbjct: 1   MLFNNLAAFPTPLTFNVVDFGTKPNNAETDSSKALQLAWMHACSSSKPATIYVPKAKFYV 60

Query: 78  HSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHNVNGVSVLGGIIDGQGSGL 137
            SATFNGPC NNDITIRIDG L+APSDFHLT NSENWI+FHNVNGV+VLGGI+DGQG+ L
Sbjct: 61  RSATFNGPCKNNDITIRIDGALMAPSDFHLTSNSENWILFHNVNGVTVLGGILDGQGTQL 120

Query: 138 WACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVVNQCHNVKMQGLKVSALGN 197
           WACK S  TCP G TSL+FTNSQNILV+GLT+LNSQ++HIVVNQC NVKMQGLKVSA GN
Sbjct: 121 WACKNSANTCPRGTTSLEFTNSQNILVNGLTALNSQLYHIVVNQCRNVKMQGLKVSAAGN 180

Query: 198 SPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIENIVCGPGHGISIGSLGKGP 257
           SPNTDGIHVG SSHVTILN+NI TGDDC+SIGPG+SNLWIENI CGPGHGISIGSL K  
Sbjct: 181 SPNTDGIHVGDSSHVTILNTNIRTGDDCISIGPGSSNLWIENIACGPGHGISIGSLAKDF 240

Query: 258 KEDGVENVTVKSCRFMNTQNG--------------------------------------- 317
           KE GV+NVTVKSC+FM+TQNG                                       
Sbjct: 241 KEKGVQNVTVKSCKFMDTQNGVRIKSWGRQSNGFARNIRFQHLTMTNVQNPIIIDQNYCP 300

Query: 318 --------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCKGIRLEDIKLTYKNQVAK 351
                    SG+ VSDV Y DI  TS T VA+KFDCSPKFPCKGI+LED+KL+YKNQVAK
Sbjct: 301 HNQGCPGQSSGVAVSDVKYTDIRGTSATPVAIKFDCSPKFPCKGIKLEDVKLSYKNQVAK 360

BLAST of Lsi03G009010 vs. NCBI nr
Match: XP_008448091.1 (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 533.1 bits (1372), Expect = 1.8e-147
Identity = 270/398 (67.84%), Postives = 298/398 (74.87%), Query Frame = 0

Query: 1   MTLHT-NSFFLLLLLLPIMTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRA 60
           M LH   SF L   L  +M F NL  F  PLTFNVV+FG KPNN + DSSKA QLAWM A
Sbjct: 1   MALHAITSFLLFSTLTVLMLFNNLAAFPTPLTFNVVDFGTKPNNAETDSSKALQLAWMHA 60

Query: 61  CTSSTPTSIYVPNAKFYVHSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHN 120
           C+SS P +IYVP AKFYV SATFNGPC NN ITIRIDG L+APSDFHLT NSENWI+FHN
Sbjct: 61  CSSSKPATIYVPKAKFYVRSATFNGPCKNNHITIRIDGALMAPSDFHLTSNSENWILFHN 120

Query: 121 VNGVSVLGGIIDGQGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVV 180
           VNGV+VLGGI+DGQG+ LWACK S  TCP G TSL+FTNSQNILV+GLT+LNSQ++HIVV
Sbjct: 121 VNGVTVLGGILDGQGTQLWACKNSANTCPRGTTSLEFTNSQNILVNGLTALNSQLYHIVV 180

Query: 181 NQCHNVKMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIEN 240
           NQC NVKMQGLKVSA GNSPNTDGIHVG SSHVTILN+NI TGDDC+SIGPG+SNLWIEN
Sbjct: 181 NQCRNVKMQGLKVSAAGNSPNTDGIHVGDSSHVTILNTNIRTGDDCISIGPGSSNLWIEN 240

Query: 241 IVCGPGHGISIGSLGKGPKEDGVENVTVKSCRFMNTQNG--------------------- 300
           I CGPGHGISIGSL K  KE GV+NVTVKSC+FM+TQNG                     
Sbjct: 241 IACGPGHGISIGSLAKDFKEKGVQNVTVKSCKFMDTQNGVRIKSWGRQSIGFARNIRFQH 300

Query: 301 --------------------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPC 351
                                      SG+ VSDV Y DI  TS T VA+KFDCSPKFPC
Sbjct: 301 LTMTNVQNPIIIDQNYCPHNQGCPGQSSGVAVSDVKYTDIRGTSATPVAIKFDCSPKFPC 360

BLAST of Lsi03G009010 vs. NCBI nr
Match: XP_023511587.1 (polygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 490.7 bits (1262), Expect = 1.0e-134
Identity = 254/389 (65.30%), Postives = 285/389 (73.26%), Query Frame = 0

Query: 1   MTLHTNSFFLLLLLLPIMTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRAC 60
           M   T SF L LLL+    FFN P FAA  TFNVVNFGAKP + K DSSKAFQ AW RAC
Sbjct: 1   MDPRTTSFLLSLLLI---IFFNSP-FAASETFNVVNFGAKP-DAKTDSSKAFQAAWARAC 60

Query: 61  TSSTPTSIYVPNAKFYVHSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHNV 120
           TSST  SIY+PN +FY+  ATFNGPC N+ I I IDGTLVAPSDFH+  NS NWIIFH V
Sbjct: 61  TSSTSASIYIPNGRFYLRHATFNGPCINHAIDIHIDGTLVAPSDFHVNANSPNWIIFHLV 120

Query: 121 NGVSVLGGIIDGQGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVVN 180
           NGVS+ GG +DGQG+GLWACK + KTCP GATSL+FTNS+NI++SGLTSLNS +FHIVVN
Sbjct: 121 NGVSIFGGTLDGQGAGLWACKRTAKTCPRGATSLEFTNSKNIMISGLTSLNSHIFHIVVN 180

Query: 181 QCHNVKMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIENI 240
           +CH+VKM+GLKVSA GNSPNTDGIHVG SSHVTILNS+I TGDDC+SIGPGTSNLWIEN+
Sbjct: 181 ECHDVKMEGLKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENV 240

Query: 241 VCGPGHGISIGSLGKGPKEDGVENVTVKSCRFMNTQNG---------------------- 300
            CGPGHGISIGSLGK  KEDGV+NVTVKSCRFMNTQNG                      
Sbjct: 241 ACGPGHGISIGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHL 300

Query: 301 -------------------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCK 343
                                     SGIKVSDVTY+DIH T+ T+ A+ FDCSPKFPC 
Sbjct: 301 KMTNVKNPIIIDQNYCPHNKDCPGQASGIKVSDVTYQDIHGTTATKFALTFDCSPKFPCV 360

BLAST of Lsi03G009010 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 380.9 bits (977), Expect = 1.1e-105
Identity = 192/380 (50.53%), Postives = 249/380 (65.53%), Query Frame = 0

Query: 14  LLPIMTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRACTSSTPTSIYVPNA 73
           LL +  FF + + +A  ++NV++FGAKP+  K D++KAF   W  AC SS P +I VP  
Sbjct: 4   LLVLFVFFFISSCSAQ-SYNVLSFGAKPDG-KTDATKAFMAVWQTACASSRPVTIVVPKG 63

Query: 74  KFYVHSATFNG-PCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHNVNGVSVLGGIIDG 133
           +F + S TF+G  C    +T RIDGTLVAP+D+ + GN + WI F +++G++V GG++D 
Sbjct: 64  RFLLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDA 123

Query: 134 QGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVVNQCHNVKMQGLKV 193
           +G+ LW CK SGK CP GAT++ F +S N++VSGLTSLNSQMFH+V+N C+NVK+QG+KV
Sbjct: 124 RGASLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKV 183

Query: 194 SALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIENIVCGPGHGISIGS 253
            A GNSPNTDGIHV  SS V+I N+ I TGDDCVSIGPGT+ LWIEN+ CGPGHGISIGS
Sbjct: 184 LAAGNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGS 243

Query: 254 LGKGPKEDGVENVTVKSCRFMNTQNG---------------------------------- 313
           LGK   E GV+NVTVK+  F  T NG                                  
Sbjct: 244 LGKDSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIID 303

Query: 314 ------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCKGIRLEDIKLTYKN 347
                        SGIK+SDV + DIH TS T+V +K DCS K PC GIRLED+KLTY+N
Sbjct: 304 QNYCPDHDCPRQVSGIKISDVLFVDIHGTSATEVGVKLDCSSKKPCTGIRLEDVKLTYQN 363

BLAST of Lsi03G009010 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 375.9 bits (964), Expect = 3.4e-104
Identity = 190/395 (48.10%), Postives = 252/395 (63.80%), Query Frame = 0

Query: 3   LHTNSFFLLLLLLPIMTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRACTS 62
           +H++   +LL LL + +       ++  T+N++++GAKP+  K DS+KAF + W +AC S
Sbjct: 1   MHSSLLIVLLFLLSVSS-------SSAQTYNILSYGAKPDG-KTDSTKAFTVLWAKACAS 60

Query: 63  STPTSIYVPNAKFYVHSATFNG-PCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHNVN 122
             P +I VP  +F + S  F+G  C    +T RI GTLVAPSD+ + G    WI+F +++
Sbjct: 61  VKPVTILVPKGRFLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLD 120

Query: 123 GVSVLGGIIDGQGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVVNQ 182
           G+SV GG++D QG+ LW+CK SGK CP GATS+ F +S+N+++SGLTSLNSQMFH+ +N 
Sbjct: 121 GISVYGGVLDAQGASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAING 180

Query: 183 CHNVKMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIENIV 242
           C NVK+ G+KVSA GNSPNTDGIHV  SS V+ILNS I TGDDCVSIGPGT+ LWIEN+ 
Sbjct: 181 CSNVKLDGVKVSADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVA 240

Query: 243 CGPGHGISIGSLGKGPKEDGVENVTVKSCRFMNTQNG----------------------- 302
           CGPGHGISIGSLGK   E GV+N+TVK+  F  T+NG                       
Sbjct: 241 CGPGHGISIGSLGKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCV 300

Query: 303 ------------------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCKG 350
                                    SGIK+SDV + DIH TS T+V +K DCS K PC G
Sbjct: 301 MNNVQNPIVIDQNYCPGNENCPNQVSGIKISDVMFFDIHGTSATEVGVKLDCSSKKPCTG 360

BLAST of Lsi03G009010 vs. TAIR 10
Match: AT2G43860.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 326.6 bits (836), Expect = 2.4e-89
Identity = 181/386 (46.89%), Postives = 239/386 (61.92%), Query Frame = 0

Query: 3   LHTNSFFLLLLLLPIMTFFNLPTFAAPL--TFNVVNFGAKPNNPKIDSSKAFQLAWMRAC 62
           ++  S  +L L L I+ F  + + A P+  T NV+++GAKP+  K DS+KAF  AW  AC
Sbjct: 2   VYNTSCLILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSK-DSTKAFLAAWDVAC 61

Query: 63  TSSTPTSIYVPNAKFYVHSATFNG-PCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHN 122
            S+ PT+I VP  +F V +  F+G  C    I+IRI G++VAP DF +  +S++WI F +
Sbjct: 62  ASANPTTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFED 121

Query: 123 VNGVSVLGGIIDGQGSGLWACKIS-GKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIV 182
           V  VS+ GGI+D QG+ LW CK + G  CP GA SL F+ S NI +SGLTS+NSQ FHIV
Sbjct: 122 VTDVSIYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIV 181

Query: 183 VNQCHNVKMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIE 242
           ++  +NV + G+KVSA  NSPNTDGIHV  S  V I NS IGTGDDC+SIGPG++N++I+
Sbjct: 182 IDNSNNVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQ 241

Query: 243 NIVCGPGHGISIGSLGKGPKEDGVENVTVKSCRFMNTQNG-------------------- 302
            I CGPGHGISIGSLG+  +E GV+NVTV +  FM T NG                    
Sbjct: 242 TIRCGPGHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQ 301

Query: 303 --------------------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPC 339
                                      SG+KVS+V YEDIH TSNT+VA+  DCS + PC
Sbjct: 302 HINMKMVKNPIIIDQHYCLHKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPC 361

BLAST of Lsi03G009010 vs. TAIR 10
Match: AT1G65570.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 321.6 bits (823), Expect = 7.7e-88
Identity = 169/387 (43.67%), Postives = 228/387 (58.91%), Query Frame = 0

Query: 12  LLLLPIMTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRACTSSTPTSIYVP 71
           +++  IM+ F    F A  + NV++FGA PN   ++S+KAF  AW  AC       IYVP
Sbjct: 13  IVITIIMSHFG--QFDARTSLNVLSFGANPNG-IVESAKAFSDAWDAACGVEDSVVIYVP 72

Query: 72  NAKFYVH-SATFNG-PCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHNVNGVSVLGGI 131
             ++ V     F G  C + +IT+RIDGTL+ P D+ L G  ENW  F  V+ V+VLGG 
Sbjct: 73  KGRYLVSGEVRFEGESCKSREITLRIDGTLIGPQDYSLLGKKENWFSFSGVHNVTVLGGS 132

Query: 132 IDGQGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVVNQCHNVKMQG 191
            D +GS LW+CK +G  CP GAT+L+F +S N+ + G+ SLNSQ+FHI +N+C N+K++ 
Sbjct: 133 FDAKGSTLWSCKANGYNCPEGATTLRFMDSNNVKIKGVLSLNSQLFHIAINRCRNIKIED 192

Query: 192 LKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIENIVCGPGHGIS 251
           +++ A   SPNTDGIH+  S+ + + N++I TGDDC+SIGPGT NL ++ I CGPGHGIS
Sbjct: 193 VRIIAPDESPNTDGIHIQLSTDIEVRNASIKTGDDCISIGPGTKNLMVDGITCGPGHGIS 252

Query: 252 IGSLGKGPKEDGVENVTVKSCRFMNTQNG------------------------------- 311
           IGSL K  +E GVENVTVK+  F+ T NG                               
Sbjct: 253 IGSLAKSIEEQGVENVTVKNAVFVRTDNGLRIKSWPRHSNGFVERVRFLGAIMVNVSYPI 312

Query: 312 ----------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCKGIRLEDIKL 350
                            SGIK++DV Y  I  TS T++A+K DCS K PC GIR++ I L
Sbjct: 313 LIDQNYCPGDSSCPSQESGIKINDVIYSGIMGTSATEIAIKMDCSEKVPCTGIRMQAINL 372

BLAST of Lsi03G009010 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 316.6 bits (810), Expect = 2.5e-86
Identity = 165/391 (42.20%), Postives = 231/391 (59.08%), Query Frame = 0

Query: 6   NSFFLLLLLLPIMTFFNLPTFAAPLTFNVVNFGAKPNNPKIDSSKAFQLAWMRACTSSTP 65
           N+  + +LL+   +F  + +  A   +NVV+FGAKP+  + DS+KAF  AW  AC S+  
Sbjct: 3   NNKLVAVLLMFFSSFLLMKSSTAASNYNVVSFGAKPDG-RTDSTKAFLGAWQAACRSAAA 62

Query: 66  TSIYVPNAKFYVHSATFNGPCNNNDITIRIDGTLVAPSDFHLTGNSENWIIFHNVNGVSV 125
            ++ VP   F +    F GPC +  IT +I GT+VAPSD+   GNS  WI+F  VN +S+
Sbjct: 63  VTVTVPRGSFLLKPVEFRGPCRSR-ITFQIYGTIVAPSDYRGLGNSGYWILFVKVNRISI 122

Query: 126 LGGIIDGQGSGLWACKISGKTCPHGATSLKFTNSQNILVSGLTSLNSQMFHIVVNQCHNV 185
           +GG +D +G+  WAC+ SGK+CP GA S+ F  + +++VSGLTS+NSQ  H+V+N C+NV
Sbjct: 123 IGGTLDARGASFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLVINSCNNV 182

Query: 186 KMQGLKVSALGNSPNTDGIHVGGSSHVTILNSNIGTGDDCVSIGPGTSNLWIENIVCGPG 245
            ++ +K+ A   SPNTDG+HV GS+ VT+ +    TGDDC+SIGPGT NL++  + CGPG
Sbjct: 183 IVRKVKLVAPDQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKLNCGPG 242

Query: 246 HGISIGSLGKGPKEDGVENVTVKSCRFMNTQNG--------------------------- 305
           HGISIGSLG+   E GVEN+T+ +  F  + NG                           
Sbjct: 243 HGISIGSLGRDANEAGVENITLINSVFSGSDNGVRIKTWARQSTGFVRNVLFQNLIMKNV 302

Query: 306 --------------------GSGIKVSDVTYEDIHETSNTQVAMKFDCSPKFPCKGIRLE 350
                               GSG+K+S V Y +I  TS TQ A+ FDCS   PC+ IRL 
Sbjct: 303 QNPIIVDQNYCPSNQGCPKQGSGVKISQVVYRNIQGTSRTQQALTFDCSRSNPCQAIRLH 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P489793.2e-11555.81Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1[more]
O228183.4e-8846.89Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386... [more]
P353385.6e-5936.46Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1[more]
P262161.6e-5835.92Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1[more]
Q6H9K06.2e-5836.59Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A0A0K6Z52.2e-15169.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G145950 PE=3 SV=1[more]
A0A5A7U8636.7e-14869.47Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A1S3BJI18.7e-14867.84polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103490378 PE=3 SV=1[more]
A0A6J1GNK76.5e-13565.55polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111455565 PE=3 SV=1[more]
A0A6J1I9T64.2e-13465.30polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470567 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038888717.14.3e-15773.05polygalacturonase-like [Benincasa hispida][more]
XP_031745066.14.6e-15169.60polygalacturonase [Cucumis sativus][more]
KAA0049805.11.4e-14769.47polygalacturonase-like [Cucumis melo var. makuwa] >TYK23060.1 polygalacturonase-... [more]
XP_008448091.11.8e-14767.84PREDICTED: polygalacturonase-like [Cucumis melo][more]
XP_023511587.11.0e-13465.30polygalacturonase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G43870.11.1e-10550.53Pectin lyase-like superfamily protein [more]
AT3G59850.13.4e-10448.10Pectin lyase-like superfamily protein [more]
AT2G43860.12.4e-8946.89Pectin lyase-like superfamily protein [more]
AT1G65570.17.7e-8843.67Pectin lyase-like superfamily protein [more]
AT2G43890.12.5e-8642.20Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 182..208
e-value: 380.0
score: 9.8
coord: 232..252
e-value: 1100.0
score: 6.8
coord: 209..230
e-value: 1200.0
score: 6.6
coord: 262..286
e-value: 20.0
score: 14.1
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 280..337
e-value: 7.8E-9
score: 35.0
coord: 60..278
e-value: 2.7E-55
score: 187.7
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 239..252
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 21..347
e-value: 3.9E-88
score: 298.1
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 8..278
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 280..349
NoneNo IPR availablePANTHERPTHR31375:SF219PUTATIVE-RELATEDcoord: 8..278
NoneNo IPR availablePANTHERPTHR31375:SF219PUTATIVE-RELATEDcoord: 280..349
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 17..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G009010.1Lsi03G009010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity