Lsi03G008830 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G008830
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGlycosyltransferase
Locationchr03: 15521162 .. 15525057 (+)
RNA-Seq ExpressionLsi03G008830
SyntenyLsi03G008830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTGTGTGAAGTATGGGATGTCATTAAGAAGGAAATTAGGATGAGTAATAAGGTGCGGGCTCATGTTGTGTGTATTCCAGTTCCACTTCAAAGCCATGTAAACGCAATGCTAAGTTTAGCAAAGCTTCTTCACCAAAGAGGCTTTTACATAACTTTTGTTCTCACAGAATACACTCATCAGCGGATCATAAGGTCTCGTGGGCCTACCTCCCTTGATGGCATACTTGATTTTCAGTTCAAAACCATTTGGGATTGTTCTATGCTTTCCATTGATTCCAATATTATTGACACTCCACAACATTTGCCTTCTCTTTGCAACTCCATTTCTAATCACTTTTTAGCCCCATTCTGTACCTTACTTTCTCAACTGATCAATAGTAATGATAATGATATCCCATCAGTTACTCGTATTGTAGCTGATGGTTTTATGTCATTCTGTATTGAAGCTGGCTTCAAATTTAACATTCCAACTGCGCAGTTTTCGCCTATTAGTGCTTGTAGCTTGCTGGGTTTTTTTCACTTTGAAGAACTCGTACAACGTGGAGTTACTCCATTTAAAGGTACTCTTATTATTATCTATAACTTTTTCTTCACTTTACGTTTATGCACTAAGTTAAGTTTTTTTTTTTTTTCTTTTGTACGACAATTATAAATACTTTAGATTTGTTATAGAAAGTATTTAAATAATATAAAAATAAAAAGCAGATGCAAATATAGCAATTAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAATAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAAATTGAGAGTCAGTTCTCAGAGTCTATCAGTGATAAGCTATATCACTAACAGGAGTGTCTAACTATCAACGGTAGAGTCTATCACTAATAGATCTATTAGTGATAGACTTTCGTTAACAAAATAGTTTTCACTAATAGGCTCTGAGAGCTAGATCTAAATTTTGCTATATCTATAGATTCTTTTATATTGTGATATACGTGCTAATACTTTGGGTCTGATTGCTATATTTGCATCTGCTTTTTATTTTTATATTATTTAAATACTTTCTATAACAAACCTAAAGTATTTATAATTGTCGTACAAAAGAAAAAAAAAAAACAATTGACTAGTTAAGTCTACTAATTAATCTAAAATTAACAACTTAACTAATATTATCTAATATTCATTCTACATGCATCACTTGTCCAATGGTTTATGAAAATGATGTATAAATGTCTTGTGAAAAATAATCAATTTTTGACTCTAAACTTAACATGTTGCATCAATTTAAACTCTAAATTTGAGGCAATTGCAAATATAGCAATTAGACTCAAAGTATTTACAGATATAGCACAATGTAAAAAAATTTGCAAATATAGCAAAATTGAGATTCAGTTCTCAGAGTCTATCAGTGATAAACTATATCACTAACAGGAGTGTCTAACTATCAACGGTAGAGTCTATCACTAATAGATTTTGTTATATTTGCAATTCTTTAGAAATGTTGCTATATATTTAATTATTAGCCCTAAAAGTAATAGCCATTAAATTTTAAACTTTGAAATGTGATGGAAGTTGTACCTTCTAAAAGTTCTAACTCCACATAGCGTTAAAAAAAATCATCTCTTATGCGTTGATTAAAATTATAAAACATGCATGATATGAGATTAATTAAAGTTCAGAGAATAAATGACATTATAGCTAAATTTTTTGTTGAAAATTTTAAATTCTAACCAAGTATTTAATAGTTAGCTTTTGCATTGATCAAAGTTATTTTCTTATTTAGTTTTTTTAATGGATTGGTCAAAGTTATTATTCAAAATTTTATTTTCATTGAAATAATAAGTTTTAAGAAACGATTTTAGAATAATTTCTAATAAAATTAGAGGGTGCATTAGCAAGAATTGAATCTATGGTAAGATGAATTACAACTATTATTTAATTTTAGGGTCATCGAAATTGAATTTTTTTTTTCCTAATTGTCTTTTGGTTTGTGTTTGCAGATGAGAATTATTTAACTAATGGTCTTCTGGAAACCACAATAGATTGGATTCCAGGGATGAAAAATGTGAGACTGAAGGATCTACCAAGCTTCATAAGAACAACTGATCCAAATGATATCTTTCTCAATTTTTTCATCCAACAATTAAAATGGTCTCCAAAAGCTTCTTGTGTTATATTAAACACTTTTGAGGCATTAGACCATGATTTCTTGGAAGCACTTTCCCACTTGTTTCCTCCAATTTACACAGTGGGTCCACTTCACTTATTTTCAACACAAATCAAGGACAAAACCCAAGAAGAAATAGCCACAAATCTTTGGGAAGAGCAACAAGAATGCATTAACTGGCTTGATTCTCAGCAACCCAACTCTGTGCTTTACGTAAATTTTGGTAGCTTAACAATAATGACACTTGAACAACTCAAAGAATTTGCTTGGGGAATTGCAAATAGTAAAAAACCCTTTTTATGGATATTAAGACCTGATGTTGTGGAAGGTGATTCACCAATTTTACCTCAAGGTTTTGTTGAGGAAACAAAGGAAAGAGGAATGATAGGGAGTTGGTGTTCACAAGTAGAGGTTTTAACTCATCCATCTATAAAAGGATTTTTGACACATAATGGATGGAATTCAACCATTGAAAGCATAAGTGCTGGAGTGCCAATGATATCATGGCCTTATTTTGGAGACCAACAAACGACATGTCGTTATTGTTCTGTGCATTGGGGGATTGCATTGGAAATAGAAAATGATGTCAAGAGAGATGAAGTTGAGAGTTGTATAAAAGAGCTAATTGAAGGAAAGAAAGGGAAAGAAATGAAAGCAAAAGTCATCGAGTTGAGAACCAAAGCTGAGGCATCGTACAAACCTGGTGGCTCTTCATACCTCAACTTTGATCAACTAATAACTCAAATTCTACTACCAACTCAG

mRNA sequence

GGTGTGTGAAGTATGGGATGTCATTAAGAAGGAAATTAGGATGAGTAATAAGGTGCGGGCTCATGTTGTGTGTATTCCAGTTCCACTTCAAAGCCATGTAAACGCAATGCTAAGTTTAGCAAAGCTTCTTCACCAAAGAGGCTTTTACATAACTTTTGTTCTCACAGAATACACTCATCAGCGGATCATAAGGTCTCGTGGGCCTACCTCCCTTGATGGCATACTTGATTTTCAGTTCAAAACCATTTGGGATTGTTCTATGCTTTCCATTGATTCCAATATTATTGACACTCCACAACATTTGCCTTCTCTTTGCAACTCCATTTCTAATCACTTTTTAGCCCCATTCTGTACCTTACTTTCTCAACTGATCAATAGTAATGATAATGATATCCCATCAGTTACTCGTATTGTAGCTGATGGTTTTATGTCATTCTGTATTGAAGCTGGCTTCAAATTTAACATTCCAACTGCGCAGTTTTCGCCTATTAGTGCTTGTAGCTTGCTGGGTTTTTTTCACTTTGAAGAACTCGTACAACGTGGAGTTACTCCATTTAAAGATTGGATTCCAGGGATGAAAAATGTGAGACTGAAGGATCTACCAAGCTTCATAAGAACAACTGATCCAAATGATATCTTTCTCAATTTTTTCATCCAACAATTAAAATGGTCTCCAAAAGCTTCTTGTGTTATATTAAACACTTTTGAGGCATTAGACCATGATTTCTTGGAAGCACTTTCCCACTTGTTTCCTCCAATTTACACAGTGGGTCCACTTCACTTATTTTCAACACAAATCAAGGACAAAACCCAAGAAGAAATAGCCACAAATCTTTGGGAAGAGCAACAAGAATGCATTAACTGGCTTGATTCTCAGCAACCCAACTCTGTGCTTTACGTAAATTTTGGTAGCTTAACAATAATGACACTTGAACAACTCAAAGAATTTGCTTGGGGAATTGCAAATAGTAAAAAACCCTTTTTATGGATATTAAGACCTGATGTTGTGGAAGGTGATTCACCAATTTTACCTCAAGGTTTTGTTGAGGAAACAAAGGAAAGAGGAATGATAGGGAGTTGGTGTTCACAAGTAGAGGTTTTAACTCATCCATCTATAAAAGGATTTTTGACACATAATGGATGGAATTCAACCATTGAAAGCATAAGTGCTGGAGTGCCAATGATATCATGGCCTTATTTTGGAGACCAACAAACGACATGTCGTTATTGTTCTGTGCATTGGGGGATTGCATTGGAAATAGAAAATGATGTCAAGAGAGATGAAGTTGAGAGTTGTATAAAAGAGCTAATTGAAGGAAAGAAAGGGAAAGAAATGAAAGCAAAAGTCATCGAGTTGAGAACCAAAGCTGAGGCATCGTACAAACCTGGTGGCTCTTCATACCTCAACTTTGATCAACTAATAACTCAAATTCTACTACCAACTCAG

Coding sequence (CDS)

ATGAGTAATAAGGTGCGGGCTCATGTTGTGTGTATTCCAGTTCCACTTCAAAGCCATGTAAACGCAATGCTAAGTTTAGCAAAGCTTCTTCACCAAAGAGGCTTTTACATAACTTTTGTTCTCACAGAATACACTCATCAGCGGATCATAAGGTCTCGTGGGCCTACCTCCCTTGATGGCATACTTGATTTTCAGTTCAAAACCATTTGGGATTGTTCTATGCTTTCCATTGATTCCAATATTATTGACACTCCACAACATTTGCCTTCTCTTTGCAACTCCATTTCTAATCACTTTTTAGCCCCATTCTGTACCTTACTTTCTCAACTGATCAATAGTAATGATAATGATATCCCATCAGTTACTCGTATTGTAGCTGATGGTTTTATGTCATTCTGTATTGAAGCTGGCTTCAAATTTAACATTCCAACTGCGCAGTTTTCGCCTATTAGTGCTTGTAGCTTGCTGGGTTTTTTTCACTTTGAAGAACTCGTACAACGTGGAGTTACTCCATTTAAAGATTGGATTCCAGGGATGAAAAATGTGAGACTGAAGGATCTACCAAGCTTCATAAGAACAACTGATCCAAATGATATCTTTCTCAATTTTTTCATCCAACAATTAAAATGGTCTCCAAAAGCTTCTTGTGTTATATTAAACACTTTTGAGGCATTAGACCATGATTTCTTGGAAGCACTTTCCCACTTGTTTCCTCCAATTTACACAGTGGGTCCACTTCACTTATTTTCAACACAAATCAAGGACAAAACCCAAGAAGAAATAGCCACAAATCTTTGGGAAGAGCAACAAGAATGCATTAACTGGCTTGATTCTCAGCAACCCAACTCTGTGCTTTACGTAAATTTTGGTAGCTTAACAATAATGACACTTGAACAACTCAAAGAATTTGCTTGGGGAATTGCAAATAGTAAAAAACCCTTTTTATGGATATTAAGACCTGATGTTGTGGAAGGTGATTCACCAATTTTACCTCAAGGTTTTGTTGAGGAAACAAAGGAAAGAGGAATGATAGGGAGTTGGTGTTCACAAGTAGAGGTTTTAACTCATCCATCTATAAAAGGATTTTTGACACATAATGGATGGAATTCAACCATTGAAAGCATAAGTGCTGGAGTGCCAATGATATCATGGCCTTATTTTGGAGACCAACAAACGACATGTCGTTATTGTTCTGTGCATTGGGGGATTGCATTGGAAATAGAAAATGATGTCAAGAGAGATGAAGTTGAGAGTTGTATAAAAGAGCTAATTGAAGGAAAGAAAGGGAAAGAAATGAAAGCAAAAGTCATCGAGTTGAGAACCAAAGCTGAGGCATCGTACAAACCTGGTGGCTCTTCATACCTCAACTTTGATCAACTAATAACTCAAATTCTACTACCAACTCAG

Protein sequence

MSNKVRAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGILDFQFKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPSVTRIVADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFKDWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHDFLEALSHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLYVNFGSLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGSWCSQVEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALEIENDVKRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQILLPTQ
Homology
BLAST of Lsi03G008830 vs. ExPASy Swiss-Prot
Match: F8WLS6 (7-deoxyloganetin glucosyltransferase OS=Catharanthus roseus OX=4058 GN=UGT85A23 PE=1 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 8.3e-151
Identity = 245/471 (52.02%), Postives = 341/471 (72.40%), Query Frame = 0

Query: 6   RAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGILDFQ 65
           + H VCIP P Q H+N ML LAKLLH +GF+ITFV TE+ H+R+++SRG  SL G+  FQ
Sbjct: 12  KPHAVCIPYPAQGHINPMLKLAKLLHYKGFHITFVNTEFNHKRLLKSRGSDSLKGLHSFQ 71

Query: 66  FKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPSVTRIV 125
           FKTI D     +  + +D  Q +PSLC S + H L PF  LL +L +++ +++P V+ +V
Sbjct: 72  FKTIPD----GLPPSDVDATQDIPSLCESTTTHCLVPFKQLLQKLNDTSSSEVPPVSCVV 131

Query: 126 ADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK------------ 185
           +D  MSF I A  + +IP   F   SAC +LG+ H+ +L+ +G+TP K            
Sbjct: 132 SDAVMSFTISAAQELDIPEVLFWTPSACGVLGYMHYAQLIDKGLTPLKDASYFSNGFLDQ 191

Query: 186 --DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHDFLE 245
             DWIPGM+ +RL+DLP+F+RTT+P++  + F +Q+ + S KAS ++LNTF+ L+ + ++
Sbjct: 192 VLDWIPGMEGIRLRDLPTFLRTTNPDEYMIKFILQETERSKKASAIVLNTFQELESEVID 251

Query: 246 ALSHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLYVNFGS 305
           +LS L PPIY +GPL +   Q+ D++ + + +NLW+E+ EC+ WLD++ PNSV+YVNFGS
Sbjct: 252 SLSTLLPPIYPIGPLQILQNQVDDESLKVLGSNLWKEEPECLEWLDTKDPNSVVYVNFGS 311

Query: 306 LTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGSWCSQV 365
           +T+MT +QL EFAWG+ANSK+ FLWI+RPD++ G+S IL + FVEETKERG+I SWC Q 
Sbjct: 312 ITVMTNDQLIEFAWGLANSKQNFLWIIRPDLISGESSILGEEFVEETKERGLIASWCHQE 371

Query: 366 EVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALEIENDV 425
           +V+ HP+I GFLTHNGWNSTIESIS+GVPMI WP+F +QQT CR+C   WGI +EI +DV
Sbjct: 372 QVINHPAIGGFLTHNGWNSTIESISSGVPMICWPFFAEQQTNCRFCCNKWGIGMEINSDV 431

Query: 426 KRDEVESCIKELIEGKKGKEMKAKVIELRTKAE-ASYKPGGSSYLNFDQLI 462
           KRDEVES +KEL+ G+KGKEMK K +E +  AE  + KP GSSY N ++LI
Sbjct: 432 KRDEVESLVKELMVGEKGKEMKKKALEWKNIAEVTTTKPDGSSYSNLEKLI 478

BLAST of Lsi03G008830 vs. ExPASy Swiss-Prot
Match: F8WKW1 (7-deoxyloganetin glucosyltransferase OS=Gardenia jasminoides OX=114476 GN=UGT85A24 PE=1 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 2.4e-150
Identity = 247/474 (52.11%), Postives = 341/474 (71.94%), Query Frame = 0

Query: 8   HVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGILDFQFK 67
           H VCIP P Q H+N ML LAK+LH +GF+ITFV TE+ H+R+++SRGP +L+G+ DFQFK
Sbjct: 11  HAVCIPYPAQGHINPMLKLAKILHHKGFHITFVNTEFNHKRLLKSRGPDALNGLPDFQFK 70

Query: 68  TIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPSVTRIVAD 127
           TI D     +  + +D  Q +PSLC S +   L PF  LL++L   + + +P V+ IV+D
Sbjct: 71  TIPD----GLPPSDVDATQDIPSLCESTTTRCLDPFRNLLAELNGPSSSQVPPVSCIVSD 130

Query: 128 GFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK-------------- 187
           G MSF +EA  +  +P   F   SAC  LG+ H+ +L+++G+TP K              
Sbjct: 131 GVMSFTLEAAAELGVPEILFWTTSACGFLGYMHYAKLIEKGLTPLKDASYLSNGYLEQSL 190

Query: 188 DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHDFLEAL 247
           DWIPGMK++RLKDLPSF+RTT+P+D  + F +Q+ + + KAS +ILNTF+ L+ D + AL
Sbjct: 191 DWIPGMKDIRLKDLPSFLRTTNPDDYMVKFVLQETERAKKASAIILNTFQELEDDVINAL 250

Query: 248 SHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLYVNFGSLT 307
           S + PPIYT+GPL     ++KD+    + +NLW+E+ EC++WLDS+ PNSV+YVNFGS+T
Sbjct: 251 SAILPPIYTIGPLQFLQKEVKDERLSVLGSNLWKEEPECLDWLDSKDPNSVVYVNFGSIT 310

Query: 308 IMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGSWCSQVEV 367
           +MT  QL EFAWG+ANSK+ FLWI+RPD+V GDS ILP  F+EETK+RG++ SWC Q +V
Sbjct: 311 VMTPGQLVEFAWGLANSKQTFLWIIRPDLVSGDSAILPPEFLEETKDRGLLASWCPQEQV 370

Query: 368 LTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALEIENDVKR 427
           L+HP+I GFLTH+GWNST+ESI +GVPMI WP+F +QQT C +C   W   LEI+N+VKR
Sbjct: 371 LSHPAIGGFLTHSGWNSTLESICSGVPMICWPFFAEQQTNCWFCCTKWYNGLEIDNNVKR 430

Query: 428 DEVESCIKELIEGKKGKEMKAKVIELRTKA-EASYKPGGSSYLNFDQLITQILL 467
           DEVES + EL+ G+KG +MK K +E + KA EA+   GGSSY N ++++ Q+LL
Sbjct: 431 DEVESLVTELMVGEKGMDMKKKALEWKNKAEEAAKSSGGSSYSNLEKVV-QVLL 479

BLAST of Lsi03G008830 vs. ExPASy Swiss-Prot
Match: Q6VAB3 (UDP-glycosyltransferase 85A8 OS=Stevia rebaudiana OX=55670 GN=UGT85A8 PE=2 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 2.5e-147
Identity = 248/476 (52.10%), Postives = 329/476 (69.12%), Query Frame = 0

Query: 6   RAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGILDFQ 65
           + H +CIP P Q H+N M+  AKLLH +GF+I+FV   Y H+R+ RSRG ++L+G+ DF 
Sbjct: 9   KPHAICIPYPAQGHINPMMQFAKLLHFKGFHISFVNNHYNHKRLQRSRGLSALEGLPDFH 68

Query: 66  FKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPSVTRIV 125
           F +I D     +  +  +  Q +P LC SI  H L PFC L++ L   N +D+P V+ I+
Sbjct: 69  FYSIPD----GLPPSNAEATQSIPGLCESIPKHSLEPFCDLIATL---NGSDVPPVSCII 128

Query: 126 ADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK------------ 185
           +DG MSF ++A  +F +P   F   SAC  L + H+ +LV +   P K            
Sbjct: 129 SDGVMSFTLQAAERFGLPEVLFWTPSACGFLAYTHYRDLVDKEYIPLKDTNDLTNGYLET 188

Query: 186 --DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHDFLE 245
             DWIPGMKN+RLKD PSFIRTTD NDI LN+F+ + +  PK   +ILNTF+AL+ D + 
Sbjct: 189 SLDWIPGMKNIRLKDFPSFIRTTDINDIMLNYFLIETEAIPKGVAIILNTFDALEKDSIT 248

Query: 246 ALSHLFPPIYTVGPLHLFSTQI-KDKTQEEIATNLWEEQQECINWLDSQQPNSVLYVNFG 305
            +  L P IYT+GPLH+    +  D+  + I +NLW+E   CINWLD+++PNSV+YVNFG
Sbjct: 249 PVLALNPQIYTIGPLHMMQQYVDHDERLKHIGSNLWKEDVSCINWLDTKKPNSVVYVNFG 308

Query: 306 SLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGSWCSQ 365
           S+T+MT EQL EF WG+ANSKK FLWI RPD+V G+  ++P  F+EETKERGM+ SWCSQ
Sbjct: 309 SITVMTKEQLIEFGWGLANSKKDFLWITRPDIVGGNEAMIPAEFIEETKERGMVTSWCSQ 368

Query: 366 VEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALEIEND 425
            EVL HPSI  FLTH+GWNSTIESIS GVPMI WP+F +QQT CRYC V W I LEI+ D
Sbjct: 369 EEVLKHPSIGVFLTHSGWNSTIESISNGVPMICWPFFAEQQTNCRYCCVEWEIGLEIDTD 428

Query: 426 VKRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQILL 467
           VKR+EVE+ ++E+++G KGK MK K +E + KAE +   GGSSYLNF++L+T +LL
Sbjct: 429 VKREEVEAQVREMMDGSKGKMMKNKALEWKKKAEEAVSIGGSSYLNFEKLVTDVLL 477

BLAST of Lsi03G008830 vs. ExPASy Swiss-Prot
Match: B2XBQ5 ((R)-mandelonitrile beta-glucosyltransferase OS=Prunus dulcis OX=3755 GN=UGT85A19 PE=1 SV=2)

HSP 1 Score: 501.5 bits (1290), Expect = 1.0e-140
Identity = 237/482 (49.17%), Postives = 340/482 (70.54%), Query Frame = 0

Query: 1   MSNKVRAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDG 60
           +++K + H V +P P Q H+N ML LAKLL+ +GF+ITFV TE+ H+R++ S+G  +LDG
Sbjct: 4   VASKEKPHAVFVPFPAQGHINPMLQLAKLLNYKGFHITFVNTEFNHKRMLESQGSHALDG 63

Query: 61  ILDFQFKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPS 120
           +  F+F+TI D     +     D  ++LP +C+S S   LAPF  LL++L +S D+  P 
Sbjct: 64  LPSFRFETIPD----GLPPADADARRNLPLVCDSTSKTCLAPFEALLTKLNSSPDS--PP 123

Query: 121 VTRIVADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK------- 180
           VT IVADG  SF ++A   F IP   F   SAC L+G+  +  L+++G+TPFK       
Sbjct: 124 VTCIVADGVTSFTLDAAEHFGIPEVLFWTTSACGLMGYVQYYRLIEKGLTPFKDAKDFAN 183

Query: 181 -------DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALD 240
                  DWIPGMK+VRLKD+PSFIRTTDPNDI L++ + + + S KAS +ILNTF+AL+
Sbjct: 184 GYLDTEIDWIPGMKDVRLKDMPSFIRTTDPNDIMLHYMVSETERSKKASAIILNTFDALE 243

Query: 241 HDFLEALSHLFPPIYTVGPLHLFSTQIKDKTQE--EIATNLWEEQQECINWLDSQQPNSV 300
            + ++ALS L PPIY++GPL L  ++I  +  +   I +NLW E  EC+NWLD+++PNSV
Sbjct: 244 QEVVDALSTLLPPIYSIGPLQLPYSEIPSEYNDLKAIGSNLWAENTECLNWLDTKEPNSV 303

Query: 301 LYVNFGSLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMI 360
           +YVNFGS T+MT EQL EF+WG+ANSKKPFLWI+RP +V G++ ++P  F+EETKERGM+
Sbjct: 304 VYVNFGSTTVMTNEQLVEFSWGLANSKKPFLWIIRPGLVAGETAVVPPEFLEETKERGML 363

Query: 361 GSWCSQVEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIA 420
            SWC Q +VL H +I GFLTH+GWNST+E++  GVP+I WP+F +QQT  RY    WGI 
Sbjct: 364 ASWCPQEQVLLHSAIGGFLTHSGWNSTLEALCGGVPLICWPFFAEQQTNVRYSCTQWGIG 423

Query: 421 LEIENDVKRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQI 467
           +EI+ +VKRD ++  ++ L++G++GK+M+ K +E +  AE +  P GSSYL  + +++++
Sbjct: 424 IEIDGEVKRDYIDGLVRTLMDGEEGKKMRKKALEWKMLAEDATAPKGSSYLALENVVSKV 479

BLAST of Lsi03G008830 vs. ExPASy Swiss-Prot
Match: Q9LMF0 (UDP-glycosyltransferase 85A5 OS=Arabidopsis thaliana OX=3702 GN=UGT85A5 PE=2 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.1e-139
Identity = 229/473 (48.41%), Postives = 327/473 (69.13%), Query Frame = 0

Query: 6   RAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGILDFQ 65
           + HVVCIP P Q H+N ML +AKLL+ RGF++TFV T Y H R+IRSRGP SLDG+  F+
Sbjct: 11  KPHVVCIPFPAQGHINPMLKVAKLLYARGFHVTFVNTNYNHNRLIRSRGPNSLDGLPSFR 70

Query: 66  FKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPSVTRIV 125
           F++I D     +     D  Q +P+LC S   + LAPF  LL ++  +   D+P V+ IV
Sbjct: 71  FESIPD----GLPEENKDVMQDVPTLCESTMKNCLAPFKELLRRI--NTTKDVPPVSCIV 130

Query: 126 ADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFKD---------WI 185
           +DG MSF ++A  +  +P   F   SAC  L + HF   +++G++P KD         WI
Sbjct: 131 SDGVMSFTLDAAEELGVPDVLFWTPSACGFLAYLHFYRFIEKGLSPIKDESSLDTKINWI 190

Query: 186 PGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHDFLEALSHL 245
           P MKN+ LKD+PSFIR T+  DI LNFF+ +   + +AS +ILNTF++L+HD + ++  +
Sbjct: 191 PSMKNLGLKDIPSFIRATNTEDIMLNFFVHEADRAKRASAIILNTFDSLEHDVVRSIQSI 250

Query: 246 FPPIYTVGPLHLFSTQIKDKTQE--EIATNLWEEQQECINWLDSQQPNSVLYVNFGSLTI 305
            P +YT+GPLHLF  +  D+  +  +I TN+W E+ EC++WLD++ PNSV+YVNFGS+T+
Sbjct: 251 IPQVYTIGPLHLFVNRDIDEESDIGQIGTNMWREEMECLDWLDTKSPNSVVYVNFGSITV 310

Query: 306 MTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGSWCSQVEVL 365
           M+ +QL EFAWG+A +KK FLW++RPD+V GD P+LP  F+ ET  R M+ SWC Q +VL
Sbjct: 311 MSAKQLVEFAWGLAATKKDFLWVIRPDLVAGDVPMLPPDFLIETANRRMLASWCPQEKVL 370

Query: 366 THPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALEIENDVKRD 425
           +HP++ GFLTH+GWNST+ES+S GVPM+ WP+F +QQT C+YC   W + +EI  DV+R+
Sbjct: 371 SHPAVGGFLTHSGWNSTLESLSGGVPMVCWPFFAEQQTNCKYCCDEWEVGMEIGGDVRRE 430

Query: 426 EVESCIKELIEGKKGKEMKAKVIELRTKAEASYKP-GGSSYLNFDQLITQILL 467
           EVE  ++EL++G KGK+M+ K  E +  AE + KP  GSS LNF  ++ ++LL
Sbjct: 431 EVEELVRELMDGDKGKKMRQKAEEWQRLAEEATKPIYGSSELNFQMVVDKVLL 477

BLAST of Lsi03G008830 vs. ExPASy TrEMBL
Match: A0A1S3BXL8 (Glycosyltransferase OS=Cucumis melo OX=3656 GN=LOC103494320 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.1e-221
Identity = 375/480 (78.12%), Postives = 416/480 (86.67%), Query Frame = 0

Query: 1   MSNKVRAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDG 60
           MS KV  H VCIPVPLQSHV+AMLS+AKLLHQRGF+IT V+T+YTH+RIIRSRGP+SLDG
Sbjct: 6   MSKKVEGHAVCIPVPLQSHVSAMLSVAKLLHQRGFFITLVITKYTHKRIIRSRGPSSLDG 65

Query: 61  ILDFQFKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPS 120
           +L+FQFKTIW+  +       ID PQ+LPSLC+SISN FL+PFC LLSQL  SND +IP 
Sbjct: 66  MLNFQFKTIWNYCV-----EPIDAPQNLPSLCDSISNDFLSPFCDLLSQL-KSND-EIPP 125

Query: 121 VTRIVADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK------- 180
           VT I+ADGFMSFCIEAG +FNIPT+QF PISACSLLGF+HFEELV+RG  PFK       
Sbjct: 126 VTCIIADGFMSFCIEAGLEFNIPTSQFWPISACSLLGFYHFEELVKRGAVPFKDESYFSN 185

Query: 181 -------DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALD 240
                  DWIPG+KNV++KDLPSFIRTTDPND FLNFFIQQLKW+PKASC ILNTFEALD
Sbjct: 186 GCMETTIDWIPGLKNVKIKDLPSFIRTTDPNDTFLNFFIQQLKWAPKASCTILNTFEALD 245

Query: 241 HDFLEALSHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLY 300
           HD LEALSHLFPPIYT+GPLHLFS QIKDKTQE+IATN WEEQQECINWLDSQQPN+V+Y
Sbjct: 246 HDVLEALSHLFPPIYTIGPLHLFSKQIKDKTQEKIATNHWEEQQECINWLDSQQPNTVIY 305

Query: 301 VNFGSLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGS 360
           +NFGSL IMTL+Q  EFAW IANS++PFLWILRP VVEG+SP LP  FVEETK RGMIG+
Sbjct: 306 INFGSLAIMTLDQCTEFAWAIANSEQPFLWILRPGVVEGNSPKLPHNFVEETKGRGMIGN 365

Query: 361 WCSQVEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALE 420
           WCSQVEVL HPSIKGFLTH+GWNSTIESISAGVPMISWP+ GDQQTTC YC VHWGIALE
Sbjct: 366 WCSQVEVLNHPSIKGFLTHSGWNSTIESISAGVPMISWPFLGDQQTTCHYCCVHWGIALE 425

Query: 421 IENDVKRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQILL 467
           I+N+VKRDEVESCIKEL EG  GKEMKAKV+ELR KAE SY PGGSSYLNFD+LITQ+LL
Sbjct: 426 IQNNVKRDEVESCIKELTEGNNGKEMKAKVMELRRKAEESYTPGGSSYLNFDRLITQLLL 478

BLAST of Lsi03G008830 vs. ExPASy TrEMBL
Match: A0A0A0LY25 (Glycosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G687630 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 4.4e-219
Identity = 366/480 (76.25%), Postives = 413/480 (86.04%), Query Frame = 0

Query: 1   MSNKVRAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDG 60
           MS +V  H VCIPVP+QSHVNAMLS+AKLLHQRGF+ITFV+TEYTH+RII SRGP+SLDG
Sbjct: 1   MSKRVEGHAVCIPVPVQSHVNAMLSVAKLLHQRGFFITFVITEYTHKRIISSRGPSSLDG 60

Query: 61  ILDFQFKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPS 120
           +L+FQFKTIWD  +       ID PQ+ PSLC+SISN FL+PFC LLSQL   N+++IP 
Sbjct: 61  LLNFQFKTIWDYCV-----EPIDAPQNFPSLCDSISNDFLSPFCDLLSQL--KNNHEIPP 120

Query: 121 VTRIVADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK------- 180
           VT I+ D FMSFCI+AG +FNIPT+QF PISACS+LG +HFEELV+RG  PFK       
Sbjct: 121 VTCIIPDAFMSFCIQAGLEFNIPTSQFWPISACSILGIYHFEELVKRGAVPFKDESYFSN 180

Query: 181 -------DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALD 240
                  DWIPGMKNV++KDLPSFIRTTDPND  LNF IQQLKW+PKASC++LNTFEALD
Sbjct: 181 GYMETTIDWIPGMKNVKMKDLPSFIRTTDPNDTLLNFCIQQLKWAPKASCIVLNTFEALD 240

Query: 241 HDFLEALSHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLY 300
           HD LEALSHLFPPIYT+GP+HLFS QIKDKTQE IATN WEEQQECI+WLDSQQP++V+Y
Sbjct: 241 HDVLEALSHLFPPIYTIGPIHLFSKQIKDKTQEMIATNHWEEQQECISWLDSQQPDTVIY 300

Query: 301 VNFGSLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGS 360
           +NFGSL I+TL+QL E AWGIANS++PFLWILRPDV+EG SP LP  FVEETK RGMIGS
Sbjct: 301 INFGSLAILTLDQLTELAWGIANSEQPFLWILRPDVLEGKSPKLPHNFVEETKGRGMIGS 360

Query: 361 WCSQVEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALE 420
           WCSQVEVL HPSIKGFLTH+GWNSTIESISAGVPMISWP+FGDQQTTC YC VHWGIALE
Sbjct: 361 WCSQVEVLNHPSIKGFLTHSGWNSTIESISAGVPMISWPFFGDQQTTCHYCCVHWGIALE 420

Query: 421 IENDVKRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQILL 467
           I+N+VKRDEVESCIKELIEG  GKEMKAKV+ELR KAE SY PGGSSYLNFD+LITQ+LL
Sbjct: 421 IQNNVKRDEVESCIKELIEGNNGKEMKAKVMELRRKAEESYTPGGSSYLNFDRLITQLLL 473

BLAST of Lsi03G008830 vs. ExPASy TrEMBL
Match: A0A6J1D6V0 (7-deoxyloganetin glucosyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111017578 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 1.3e-194
Identity = 330/476 (69.33%), Postives = 388/476 (81.51%), Query Frame = 0

Query: 6   RAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGILDFQ 65
           ++HVVCIP P+QS +NAMLS+AKLLHQ+GFYITFV TEYTH+RII+SRGP SLDG  +FQ
Sbjct: 10  KSHVVCIPFPVQSRINAMLSVAKLLHQKGFYITFVNTEYTHKRIIKSRGPNSLDGTHNFQ 69

Query: 66  FKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDI-PSVTRI 125
           FKTI D   +   SN ID PQ LPSLC S+  +FLAPFC LLSQL +++ + I P ++ I
Sbjct: 70  FKTIPD--GMPPPSN-IDAPQDLPSLCISVCENFLAPFCNLLSQLNDASSHSIVPPISNI 129

Query: 126 VADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK----------- 185
           + DG MSF ++A  +FN+P  QF  +SACSL+GF H+ ELV+RGVTPFK           
Sbjct: 130 IGDGCMSFSVQAALQFNLPITQFWXVSACSLIGFMHYRELVERGVTPFKDESYLTNGCLE 189

Query: 186 ---DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHDFL 245
              DWIPGMKN+RLKDLPSFIRTTDP+DIFLNFFIQQ KW+PKAS +ILNT++ALD + L
Sbjct: 190 TIMDWIPGMKNIRLKDLPSFIRTTDPDDIFLNFFIQQTKWTPKASAIILNTYDALDQEVL 249

Query: 246 EALSHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLYVNFG 305
           +ALS   PPIYT+GPLHLF+ QI     EEIATNLWEE  ECINWLDS++ NSV+Y+NFG
Sbjct: 250 DALSCFLPPIYTIGPLHLFARQINGGGLEEIATNLWEENGECINWLDSKESNSVIYINFG 309

Query: 306 SLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGSWCSQ 365
           SLT+MTL  L EFAWG+ANSKKPFLWI+RPD+V   S ILP  F+EETK+RGMI SWCSQ
Sbjct: 310 SLTVMTLHHLTEFAWGLANSKKPFLWIIRPDLVNDGSIILPHDFLEETKKRGMIASWCSQ 369

Query: 366 VEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALEIEND 425
            EVL HPSIKGFLTHNGWNST+ESIS GVPMISWP+FGDQQTTCRYC V WGIALEI+ND
Sbjct: 370 EEVLKHPSIKGFLTHNGWNSTMESISGGVPMISWPFFGDQQTTCRYCCVEWGIALEIDND 429

Query: 426 VKRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQILL 467
           VKRD+VESCIKEL+EGKKG+EMK  V+EL+ KAE SY PGGSSYLNFD+L+ +ILL
Sbjct: 430 VKRDKVESCIKELMEGKKGEEMKKNVMELKRKAEESYLPGGSSYLNFDKLVVEILL 482

BLAST of Lsi03G008830 vs. ExPASy TrEMBL
Match: A0A6J1DRI0 (7-deoxyloganetin glucosyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111023696 PE=4 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 3.7e-194
Identity = 328/475 (69.05%), Postives = 387/475 (81.47%), Query Frame = 0

Query: 6   RAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGILDFQ 65
           + HVVCIP+P+QSH+NAMLS+AKLLHQRGFYITFV TEYT  RII+SRG  SL+G  +FQ
Sbjct: 10  KPHVVCIPLPVQSHINAMLSVAKLLHQRGFYITFVNTEYTQNRIIKSRGLNSLNGTHNFQ 69

Query: 66  FKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPSVTRIV 125
           FKTI D       S+ ID PQ LPSLC ++  +FL PFC LLSQL +++ + IP V+ ++
Sbjct: 70  FKTIPDGMP---PSSHIDAPQDLPSLCIAVCKNFLTPFCNLLSQLNDASSHSIPPVSCVI 129

Query: 126 ADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK------------ 185
            DG MSF ++A  +F IP AQF P+SACSL+GF HF ELV+RGVTPFK            
Sbjct: 130 GDGCMSFSVQAALQFGIPIAQFWPVSACSLIGFMHFRELVERGVTPFKDESYLTNGCLET 189

Query: 186 --DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHDFLE 245
             DWIPGMKN+RLKDLPSFIRTTDPNDIFLNFFIQQ+KW+PKAS +ILNT++ALD + L+
Sbjct: 190 IIDWIPGMKNIRLKDLPSFIRTTDPNDIFLNFFIQQMKWTPKASAIILNTYDALDQEVLD 249

Query: 246 ALSHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLYVNFGS 305
           ALS   PPIYT+GPLHLF+ QI     EEIATNLWEE  ECINWLDS++ NSV+Y+NFGS
Sbjct: 250 ALSCFLPPIYTIGPLHLFARQINGGGLEEIATNLWEENGECINWLDSKESNSVIYINFGS 309

Query: 306 LTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGSWCSQV 365
           LT+MTL  L EFAWG+ANSKKPFLWI+RPD+V+  S ILP  F+EETKERGMI SWCSQ 
Sbjct: 310 LTVMTLHHLTEFAWGLANSKKPFLWIIRPDLVDDGSIILPHDFLEETKERGMIASWCSQE 369

Query: 366 EVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALEIENDV 425
           EVL HPSIKGFLTHNGWNST+ESISAGVPMIS P+FGDQQTTCRYC V WGIALEI++DV
Sbjct: 370 EVLKHPSIKGFLTHNGWNSTMESISAGVPMISRPFFGDQQTTCRYCCVEWGIALEIDDDV 429

Query: 426 KRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQILL 467
           KRD+VESCIKEL+EGKKG+EMK  ++EL+ KA+ SY PGGSSYLNFD+L+ +ILL
Sbjct: 430 KRDQVESCIKELMEGKKGEEMKKNIMELKRKAKESYIPGGSSYLNFDKLVVEILL 481

BLAST of Lsi03G008830 vs. ExPASy TrEMBL
Match: A0A6J1JKN3 (7-deoxyloganetin glucosyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111486659 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.9e-182
Identity = 315/476 (66.18%), Postives = 379/476 (79.62%), Query Frame = 0

Query: 6   RAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGILDFQ 65
           + H VC+P P  SHV AMLS+AKLLHQRGF+ITFV+T+Y ++RII+SRGP SLDGIL+FQ
Sbjct: 6   KPHAVCVPHPSLSHVKAMLSVAKLLHQRGFFITFVITDYVYKRIIKSRGPNSLDGILNFQ 65

Query: 66  FKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPSVTRIV 125
           FKTI D  ++S D++ I+  Q L S+C +ISNHFL+PF +LLSQL   N    P V+ IV
Sbjct: 66  FKTIAD-GIMSEDTD-IEAAQDLASICIAISNHFLSPFRSLLSQL---NHEPTPPVSCIV 125

Query: 126 ADGFM-SFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK----------- 185
           +DG M SF I+A  +FN+P AQ  PIS CSLLG  HF+ELV+RGVTPF+           
Sbjct: 126 SDGCMSSFTIKAAMEFNVPIAQLWPISPCSLLGCMHFDELVKRGVTPFQDESYLSNGYLN 185

Query: 186 ---DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHDFL 245
              DWI GMKN++LK LPS IRTTDPN+I LNF ++Q KW+ KAS +ILNTF+ALDH+ L
Sbjct: 186 TTIDWISGMKNIKLKYLPSLIRTTDPNNIILNFLVEQTKWTRKASAIILNTFDALDHEVL 245

Query: 246 EALSHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLYVNFG 305
           +ALS + PPIYT+GPLHL S QIKD++ E IATN WEE  EC+ WLDS++PNSV+YVNFG
Sbjct: 246 DALSSMLPPIYTIGPLHLLSRQIKDQSLEAIATNFWEENHECVTWLDSKEPNSVIYVNFG 305

Query: 306 SLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGSWCSQ 365
           S+TIMTL Q+ EFAWG+ANSKKPFLWI+RPD+V+ DSPIL   FVEETK  GM+ SWC+Q
Sbjct: 306 SMTIMTLNQITEFAWGLANSKKPFLWIIRPDLVKSDSPILSHDFVEETKGIGMVASWCAQ 365

Query: 366 VEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALEIEND 425
            EVL HPSIKGFLTHNGWNST+ESI  GVPMISWPYFGDQ TTC YC   WGIALEI+ND
Sbjct: 366 EEVLKHPSIKGFLTHNGWNSTLESICVGVPMISWPYFGDQPTTCLYCCEKWGIALEIDND 425

Query: 426 VKRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQILL 467
           VKRD VESCIKEL+EGKKG++MKA+V+EL++KAE S + GGSSYLN D LIT +LL
Sbjct: 426 VKRDGVESCIKELMEGKKGEDMKAEVMELKSKAEESCRHGGSSYLNLDTLITDVLL 476

BLAST of Lsi03G008830 vs. NCBI nr
Match: XP_008453670.2 (PREDICTED: 7-deoxyloganetin glucosyltransferase-like [Cucumis melo])

HSP 1 Score: 778.1 bits (2008), Expect = 4.3e-221
Identity = 375/480 (78.12%), Postives = 416/480 (86.67%), Query Frame = 0

Query: 1   MSNKVRAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDG 60
           MS KV  H VCIPVPLQSHV+AMLS+AKLLHQRGF+IT V+T+YTH+RIIRSRGP+SLDG
Sbjct: 6   MSKKVEGHAVCIPVPLQSHVSAMLSVAKLLHQRGFFITLVITKYTHKRIIRSRGPSSLDG 65

Query: 61  ILDFQFKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPS 120
           +L+FQFKTIW+  +       ID PQ+LPSLC+SISN FL+PFC LLSQL  SND +IP 
Sbjct: 66  MLNFQFKTIWNYCV-----EPIDAPQNLPSLCDSISNDFLSPFCDLLSQL-KSND-EIPP 125

Query: 121 VTRIVADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK------- 180
           VT I+ADGFMSFCIEAG +FNIPT+QF PISACSLLGF+HFEELV+RG  PFK       
Sbjct: 126 VTCIIADGFMSFCIEAGLEFNIPTSQFWPISACSLLGFYHFEELVKRGAVPFKDESYFSN 185

Query: 181 -------DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALD 240
                  DWIPG+KNV++KDLPSFIRTTDPND FLNFFIQQLKW+PKASC ILNTFEALD
Sbjct: 186 GCMETTIDWIPGLKNVKIKDLPSFIRTTDPNDTFLNFFIQQLKWAPKASCTILNTFEALD 245

Query: 241 HDFLEALSHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLY 300
           HD LEALSHLFPPIYT+GPLHLFS QIKDKTQE+IATN WEEQQECINWLDSQQPN+V+Y
Sbjct: 246 HDVLEALSHLFPPIYTIGPLHLFSKQIKDKTQEKIATNHWEEQQECINWLDSQQPNTVIY 305

Query: 301 VNFGSLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGS 360
           +NFGSL IMTL+Q  EFAW IANS++PFLWILRP VVEG+SP LP  FVEETK RGMIG+
Sbjct: 306 INFGSLAIMTLDQCTEFAWAIANSEQPFLWILRPGVVEGNSPKLPHNFVEETKGRGMIGN 365

Query: 361 WCSQVEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALE 420
           WCSQVEVL HPSIKGFLTH+GWNSTIESISAGVPMISWP+ GDQQTTC YC VHWGIALE
Sbjct: 366 WCSQVEVLNHPSIKGFLTHSGWNSTIESISAGVPMISWPFLGDQQTTCHYCCVHWGIALE 425

Query: 421 IENDVKRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQILL 467
           I+N+VKRDEVESCIKEL EG  GKEMKAKV+ELR KAE SY PGGSSYLNFD+LITQ+LL
Sbjct: 426 IQNNVKRDEVESCIKELTEGNNGKEMKAKVMELRRKAEESYTPGGSSYLNFDRLITQLLL 478

BLAST of Lsi03G008830 vs. NCBI nr
Match: KAE8653615.1 (hypothetical protein Csa_006847 [Cucumis sativus])

HSP 1 Score: 770.4 bits (1988), Expect = 9.0e-219
Identity = 366/480 (76.25%), Postives = 413/480 (86.04%), Query Frame = 0

Query: 1   MSNKVRAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDG 60
           MS +V  H VCIPVP+QSHVNAMLS+AKLLHQRGF+ITFV+TEYTH+RII SRGP+SLDG
Sbjct: 17  MSKRVEGHAVCIPVPVQSHVNAMLSVAKLLHQRGFFITFVITEYTHKRIISSRGPSSLDG 76

Query: 61  ILDFQFKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPS 120
           +L+FQFKTIWD  +       ID PQ+ PSLC+SISN FL+PFC LLSQL   N+++IP 
Sbjct: 77  LLNFQFKTIWDYCV-----EPIDAPQNFPSLCDSISNDFLSPFCDLLSQL--KNNHEIPP 136

Query: 121 VTRIVADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK------- 180
           VT I+ D FMSFCI+AG +FNIPT+QF PISACS+LG +HFEELV+RG  PFK       
Sbjct: 137 VTCIIPDAFMSFCIQAGLEFNIPTSQFWPISACSILGIYHFEELVKRGAVPFKDESYFSN 196

Query: 181 -------DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALD 240
                  DWIPGMKNV++KDLPSFIRTTDPND  LNF IQQLKW+PKASC++LNTFEALD
Sbjct: 197 GYMETTIDWIPGMKNVKMKDLPSFIRTTDPNDTLLNFCIQQLKWAPKASCIVLNTFEALD 256

Query: 241 HDFLEALSHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLY 300
           HD LEALSHLFPPIYT+GP+HLFS QIKDKTQE IATN WEEQQECI+WLDSQQP++V+Y
Sbjct: 257 HDVLEALSHLFPPIYTIGPIHLFSKQIKDKTQEMIATNHWEEQQECISWLDSQQPDTVIY 316

Query: 301 VNFGSLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGS 360
           +NFGSL I+TL+QL E AWGIANS++PFLWILRPDV+EG SP LP  FVEETK RGMIGS
Sbjct: 317 INFGSLAILTLDQLTELAWGIANSEQPFLWILRPDVLEGKSPKLPHNFVEETKGRGMIGS 376

Query: 361 WCSQVEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALE 420
           WCSQVEVL HPSIKGFLTH+GWNSTIESISAGVPMISWP+FGDQQTTC YC VHWGIALE
Sbjct: 377 WCSQVEVLNHPSIKGFLTHSGWNSTIESISAGVPMISWPFFGDQQTTCHYCCVHWGIALE 436

Query: 421 IENDVKRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQILL 467
           I+N+VKRDEVESCIKELIEG  GKEMKAKV+ELR KAE SY PGGSSYLNFD+LITQ+LL
Sbjct: 437 IQNNVKRDEVESCIKELIEGNNGKEMKAKVMELRRKAEESYTPGGSSYLNFDRLITQLLL 489

BLAST of Lsi03G008830 vs. NCBI nr
Match: XP_004142536.1 (7-deoxyloganetin glucosyltransferase [Cucumis sativus])

HSP 1 Score: 770.4 bits (1988), Expect = 9.0e-219
Identity = 366/480 (76.25%), Postives = 413/480 (86.04%), Query Frame = 0

Query: 1   MSNKVRAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDG 60
           MS +V  H VCIPVP+QSHVNAMLS+AKLLHQRGF+ITFV+TEYTH+RII SRGP+SLDG
Sbjct: 1   MSKRVEGHAVCIPVPVQSHVNAMLSVAKLLHQRGFFITFVITEYTHKRIISSRGPSSLDG 60

Query: 61  ILDFQFKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPS 120
           +L+FQFKTIWD  +       ID PQ+ PSLC+SISN FL+PFC LLSQL   N+++IP 
Sbjct: 61  LLNFQFKTIWDYCV-----EPIDAPQNFPSLCDSISNDFLSPFCDLLSQL--KNNHEIPP 120

Query: 121 VTRIVADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK------- 180
           VT I+ D FMSFCI+AG +FNIPT+QF PISACS+LG +HFEELV+RG  PFK       
Sbjct: 121 VTCIIPDAFMSFCIQAGLEFNIPTSQFWPISACSILGIYHFEELVKRGAVPFKDESYFSN 180

Query: 181 -------DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALD 240
                  DWIPGMKNV++KDLPSFIRTTDPND  LNF IQQLKW+PKASC++LNTFEALD
Sbjct: 181 GYMETTIDWIPGMKNVKMKDLPSFIRTTDPNDTLLNFCIQQLKWAPKASCIVLNTFEALD 240

Query: 241 HDFLEALSHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLY 300
           HD LEALSHLFPPIYT+GP+HLFS QIKDKTQE IATN WEEQQECI+WLDSQQP++V+Y
Sbjct: 241 HDVLEALSHLFPPIYTIGPIHLFSKQIKDKTQEMIATNHWEEQQECISWLDSQQPDTVIY 300

Query: 301 VNFGSLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGS 360
           +NFGSL I+TL+QL E AWGIANS++PFLWILRPDV+EG SP LP  FVEETK RGMIGS
Sbjct: 301 INFGSLAILTLDQLTELAWGIANSEQPFLWILRPDVLEGKSPKLPHNFVEETKGRGMIGS 360

Query: 361 WCSQVEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALE 420
           WCSQVEVL HPSIKGFLTH+GWNSTIESISAGVPMISWP+FGDQQTTC YC VHWGIALE
Sbjct: 361 WCSQVEVLNHPSIKGFLTHSGWNSTIESISAGVPMISWPFFGDQQTTCHYCCVHWGIALE 420

Query: 421 IENDVKRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQILL 467
           I+N+VKRDEVESCIKELIEG  GKEMKAKV+ELR KAE SY PGGSSYLNFD+LITQ+LL
Sbjct: 421 IQNNVKRDEVESCIKELIEGNNGKEMKAKVMELRRKAEESYTPGGSSYLNFDRLITQLLL 473

BLAST of Lsi03G008830 vs. NCBI nr
Match: XP_022149077.1 (7-deoxyloganetin glucosyltransferase-like [Momordica charantia])

HSP 1 Score: 689.1 bits (1777), Expect = 2.6e-194
Identity = 330/476 (69.33%), Postives = 388/476 (81.51%), Query Frame = 0

Query: 6   RAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGILDFQ 65
           ++HVVCIP P+QS +NAMLS+AKLLHQ+GFYITFV TEYTH+RII+SRGP SLDG  +FQ
Sbjct: 10  KSHVVCIPFPVQSRINAMLSVAKLLHQKGFYITFVNTEYTHKRIIKSRGPNSLDGTHNFQ 69

Query: 66  FKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDI-PSVTRI 125
           FKTI D   +   SN ID PQ LPSLC S+  +FLAPFC LLSQL +++ + I P ++ I
Sbjct: 70  FKTIPD--GMPPPSN-IDAPQDLPSLCISVCENFLAPFCNLLSQLNDASSHSIVPPISNI 129

Query: 126 VADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK----------- 185
           + DG MSF ++A  +FN+P  QF  +SACSL+GF H+ ELV+RGVTPFK           
Sbjct: 130 IGDGCMSFSVQAALQFNLPITQFWXVSACSLIGFMHYRELVERGVTPFKDESYLTNGCLE 189

Query: 186 ---DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHDFL 245
              DWIPGMKN+RLKDLPSFIRTTDP+DIFLNFFIQQ KW+PKAS +ILNT++ALD + L
Sbjct: 190 TIMDWIPGMKNIRLKDLPSFIRTTDPDDIFLNFFIQQTKWTPKASAIILNTYDALDQEVL 249

Query: 246 EALSHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLYVNFG 305
           +ALS   PPIYT+GPLHLF+ QI     EEIATNLWEE  ECINWLDS++ NSV+Y+NFG
Sbjct: 250 DALSCFLPPIYTIGPLHLFARQINGGGLEEIATNLWEENGECINWLDSKESNSVIYINFG 309

Query: 306 SLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGSWCSQ 365
           SLT+MTL  L EFAWG+ANSKKPFLWI+RPD+V   S ILP  F+EETK+RGMI SWCSQ
Sbjct: 310 SLTVMTLHHLTEFAWGLANSKKPFLWIIRPDLVNDGSIILPHDFLEETKKRGMIASWCSQ 369

Query: 366 VEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALEIEND 425
            EVL HPSIKGFLTHNGWNST+ESIS GVPMISWP+FGDQQTTCRYC V WGIALEI+ND
Sbjct: 370 EEVLKHPSIKGFLTHNGWNSTMESISGGVPMISWPFFGDQQTTCRYCCVEWGIALEIDND 429

Query: 426 VKRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQILL 467
           VKRD+VESCIKEL+EGKKG+EMK  V+EL+ KAE SY PGGSSYLNFD+L+ +ILL
Sbjct: 430 VKRDKVESCIKELMEGKKGEEMKKNVMELKRKAEESYLPGGSSYLNFDKLVVEILL 482

BLAST of Lsi03G008830 vs. NCBI nr
Match: XP_022156855.1 (7-deoxyloganetin glucosyltransferase-like [Momordica charantia])

HSP 1 Score: 687.6 bits (1773), Expect = 7.7e-194
Identity = 328/475 (69.05%), Postives = 387/475 (81.47%), Query Frame = 0

Query: 6   RAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGILDFQ 65
           + HVVCIP+P+QSH+NAMLS+AKLLHQRGFYITFV TEYT  RII+SRG  SL+G  +FQ
Sbjct: 10  KPHVVCIPLPVQSHINAMLSVAKLLHQRGFYITFVNTEYTQNRIIKSRGLNSLNGTHNFQ 69

Query: 66  FKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPSVTRIV 125
           FKTI D       S+ ID PQ LPSLC ++  +FL PFC LLSQL +++ + IP V+ ++
Sbjct: 70  FKTIPDGMP---PSSHIDAPQDLPSLCIAVCKNFLTPFCNLLSQLNDASSHSIPPVSCVI 129

Query: 126 ADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK------------ 185
            DG MSF ++A  +F IP AQF P+SACSL+GF HF ELV+RGVTPFK            
Sbjct: 130 GDGCMSFSVQAALQFGIPIAQFWPVSACSLIGFMHFRELVERGVTPFKDESYLTNGCLET 189

Query: 186 --DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHDFLE 245
             DWIPGMKN+RLKDLPSFIRTTDPNDIFLNFFIQQ+KW+PKAS +ILNT++ALD + L+
Sbjct: 190 IIDWIPGMKNIRLKDLPSFIRTTDPNDIFLNFFIQQMKWTPKASAIILNTYDALDQEVLD 249

Query: 246 ALSHLFPPIYTVGPLHLFSTQIKDKTQEEIATNLWEEQQECINWLDSQQPNSVLYVNFGS 305
           ALS   PPIYT+GPLHLF+ QI     EEIATNLWEE  ECINWLDS++ NSV+Y+NFGS
Sbjct: 250 ALSCFLPPIYTIGPLHLFARQINGGGLEEIATNLWEENGECINWLDSKESNSVIYINFGS 309

Query: 306 LTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGSWCSQV 365
           LT+MTL  L EFAWG+ANSKKPFLWI+RPD+V+  S ILP  F+EETKERGMI SWCSQ 
Sbjct: 310 LTVMTLHHLTEFAWGLANSKKPFLWIIRPDLVDDGSIILPHDFLEETKERGMIASWCSQE 369

Query: 366 EVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALEIENDV 425
           EVL HPSIKGFLTHNGWNST+ESISAGVPMIS P+FGDQQTTCRYC V WGIALEI++DV
Sbjct: 370 EVLKHPSIKGFLTHNGWNSTMESISAGVPMISRPFFGDQQTTCRYCCVEWGIALEIDDDV 429

Query: 426 KRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYKPGGSSYLNFDQLITQILL 467
           KRD+VESCIKEL+EGKKG+EMK  ++EL+ KA+ SY PGGSSYLNFD+L+ +ILL
Sbjct: 430 KRDQVESCIKELMEGKKGEEMKKNIMELKRKAKESYIPGGSSYLNFDKLVVEILL 481

BLAST of Lsi03G008830 vs. TAIR 10
Match: AT1G22370.2 (UDP-glucosyl transferase 85A5 )

HSP 1 Score: 498.0 bits (1281), Expect = 8.0e-141
Identity = 229/473 (48.41%), Postives = 327/473 (69.13%), Query Frame = 0

Query: 6   RAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGILDFQ 65
           + HVVCIP P Q H+N ML +AKLL+ RGF++TFV T Y H R+IRSRGP SLDG+  F+
Sbjct: 11  KPHVVCIPFPAQGHINPMLKVAKLLYARGFHVTFVNTNYNHNRLIRSRGPNSLDGLPSFR 70

Query: 66  FKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPSVTRIV 125
           F++I D     +     D  Q +P+LC S   + LAPF  LL ++  +   D+P V+ IV
Sbjct: 71  FESIPD----GLPEENKDVMQDVPTLCESTMKNCLAPFKELLRRI--NTTKDVPPVSCIV 130

Query: 126 ADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFKD---------WI 185
           +DG MSF ++A  +  +P   F   SAC  L + HF   +++G++P KD         WI
Sbjct: 131 SDGVMSFTLDAAEELGVPDVLFWTPSACGFLAYLHFYRFIEKGLSPIKDESSLDTKINWI 190

Query: 186 PGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHDFLEALSHL 245
           P MKN+ LKD+PSFIR T+  DI LNFF+ +   + +AS +ILNTF++L+HD + ++  +
Sbjct: 191 PSMKNLGLKDIPSFIRATNTEDIMLNFFVHEADRAKRASAIILNTFDSLEHDVVRSIQSI 250

Query: 246 FPPIYTVGPLHLFSTQIKDKTQE--EIATNLWEEQQECINWLDSQQPNSVLYVNFGSLTI 305
            P +YT+GPLHLF  +  D+  +  +I TN+W E+ EC++WLD++ PNSV+YVNFGS+T+
Sbjct: 251 IPQVYTIGPLHLFVNRDIDEESDIGQIGTNMWREEMECLDWLDTKSPNSVVYVNFGSITV 310

Query: 306 MTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGSWCSQVEVL 365
           M+ +QL EFAWG+A +KK FLW++RPD+V GD P+LP  F+ ET  R M+ SWC Q +VL
Sbjct: 311 MSAKQLVEFAWGLAATKKDFLWVIRPDLVAGDVPMLPPDFLIETANRRMLASWCPQEKVL 370

Query: 366 THPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALEIENDVKRD 425
           +HP++ GFLTH+GWNST+ES+S GVPM+ WP+F +QQT C+YC   W + +EI  DV+R+
Sbjct: 371 SHPAVGGFLTHSGWNSTLESLSGGVPMVCWPFFAEQQTNCKYCCDEWEVGMEIGGDVRRE 430

Query: 426 EVESCIKELIEGKKGKEMKAKVIELRTKAEASYKP-GGSSYLNFDQLITQILL 467
           EVE  ++EL++G KGK+M+ K  E +  AE + KP  GSS LNF  ++ ++LL
Sbjct: 431 EVEELVRELMDGDKGKKMRQKAEEWQRLAEEATKPIYGSSELNFQMVVDKVLL 477

BLAST of Lsi03G008830 vs. TAIR 10
Match: AT1G22380.1 (UDP-glucosyl transferase 85A3 )

HSP 1 Score: 479.2 bits (1232), Expect = 3.9e-135
Identity = 218/483 (45.13%), Postives = 327/483 (67.70%), Query Frame = 0

Query: 1   MSNKVRAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDG 60
           +SN+ + HVVC+P P Q H+N M+ +AKLLH +GF++TFV T Y H R++RSRG  +LDG
Sbjct: 6   VSNEQKPHVVCVPYPAQGHINPMMKVAKLLHVKGFHVTFVNTVYNHNRLLRSRGANALDG 65

Query: 61  ILDFQFKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPS 120
           +  FQF++I D     +    +D  Q +P+L  S + + L PF  LL +++     D+P 
Sbjct: 66  LPSFQFESIPD----GLPETGVDATQDIPALSESTTKNCLVPFKKLLQRIVTR--EDVPP 125

Query: 121 VTRIVADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK------- 180
           V+ IV+DG MSF ++   +  +P   F   SAC  + + HF   +++G+ P K       
Sbjct: 126 VSCIVSDGSMSFTLDVAEELGVPEIHFWTTSACGFMAYLHFYLFIEKGLCPVKDASCLTK 185

Query: 181 -------DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALD 240
                  DWIP M NV+LKD+PSFIRTT+PNDI LNF +++   + +AS +ILNTF+ L+
Sbjct: 186 EYLDTVIDWIPSMNNVKLKDIPSFIRTTNPNDIMLNFVVREACRTKRASAIILNTFDDLE 245

Query: 241 HDFLEALSHLFPPIYTVGPLHLFSTQIKDKTQE--EIATNLWEEQQECINWLDSQQPNSV 300
           HD ++++  + PP+Y +GPLHL   +  ++  E   + +NLW+E+ EC+ WL+++  NSV
Sbjct: 246 HDIIQSMQSILPPVYPIGPLHLLVNREIEEDSEIGRMGSNLWKEETECLGWLNTKSRNSV 305

Query: 301 LYVNFGSLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMI 360
           +YVNFGS+TIMT  QL EFAWG+A + K FLW++RPD V G+  ++P+ F+ ET +R M+
Sbjct: 306 VYVNFGSITIMTTAQLLEFAWGLAATGKEFLWVMRPDSVAGEEAVIPKEFLAETADRRML 365

Query: 361 GSWCSQVEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIA 420
            SWC Q +VL+HP++ GFLTH GWNST+ES+S GVPM+ WP+F +QQT C++    W + 
Sbjct: 366 TSWCPQEKVLSHPAVGGFLTHCGWNSTLESLSCGVPMVCWPFFAEQQTNCKFSCDEWEVG 425

Query: 421 LEIENDVKRDEVESCIKELIEGKKGKEMKAKVIELRTKAEASYK-PGGSSYLNFDQLITQ 467
           +EI  DVKR EVE+ ++EL++G+KGK+M+ K +E R  AE + K P GSS +NF+ ++ +
Sbjct: 426 IEIGGDVKRGEVEAVVRELMDGEKGKKMREKAVEWRRLAEKATKLPCGSSVINFETIVNK 482

BLAST of Lsi03G008830 vs. TAIR 10
Match: AT1G22340.1 (UDP-glucosyl transferase 85A7 )

HSP 1 Score: 478.8 bits (1231), Expect = 5.0e-135
Identity = 225/482 (46.68%), Postives = 334/482 (69.29%), Query Frame = 0

Query: 3   NKVRAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGIL 62
           N  + HVVC+P P Q H+N ML +AKLL+ +GF++TFV T Y H R++RSRGP +LDG  
Sbjct: 8   NAQKPHVVCVPYPAQGHINPMLKVAKLLYAKGFHVTFVNTLYNHNRLLRSRGPNALDGFP 67

Query: 63  DFQFKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPSVT 122
            F+F++I D     +     D  QH P++C SI  + LAPF  +L + IN  D D+P V+
Sbjct: 68  SFRFESIPD----GLPETDGDRTQHTPTVCMSIEKNCLAPFKEILRR-INDKD-DVPPVS 127

Query: 123 RIVADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK--------- 182
            IV+DG MSF ++A  +  +P   F   SAC  +   HF   +++G++PFK         
Sbjct: 128 CIVSDGVMSFTLDAAEELGVPEVIFWTNSACGFMTILHFYLFIEKGLSPFKDESYMSKEH 187

Query: 183 -----DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHD 242
                DWIP MKN+RLKD+PS+IRTT+P++I LNF I++++ S +AS +ILNTF+ L+HD
Sbjct: 188 LDTVIDWIPSMKNLRLKDIPSYIRTTNPDNIMLNFLIREVERSKRASAIILNTFDELEHD 247

Query: 243 FLEALSHLFPPIYTVGPLHLFSTQIKDKTQE--EIATNLWEEQQECINWLDSQQPNSVLY 302
            ++++  + PP+Y++GPLHL   +  ++  E  ++  NLW E+ EC++WLD++ PNSVL+
Sbjct: 248 VIQSMQSILPPVYSIGPLHLLVKEEINEASEIGQMGLNLWREEMECLDWLDTKTPNSVLF 307

Query: 303 VNFGSLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDS-PILPQGFVEETKERGMIG 362
           VNFG +T+M+ +QL+EFAWG+A S+K FLW++RP++V G++  +LPQ F+ ET +R M+ 
Sbjct: 308 VNFGCITVMSAKQLEEFAWGLAASRKEFLWVIRPNLVVGEAMVVLPQEFLAETIDRRMLA 367

Query: 363 SWCSQVEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIAL 422
           SWC Q +VL+HP+I GFLTH GWNST+ES++ GVPMI WP F +Q T C++C   WG+ +
Sbjct: 368 SWCPQEKVLSHPAIGGFLTHCGWNSTLESLAGGVPMICWPCFSEQPTNCKFCCDEWGVGI 427

Query: 423 EIENDVKRDEVESCIKELIEGKKGKEMKAKVIELRTKA-EASYKPGGSSYLNFDQLITQI 467
           EI  DVKR+EVE+ ++EL++G+KGK+++ K  E R  A EA+    GSS +N + LI ++
Sbjct: 428 EIGKDVKREEVETVVRELMDGEKGKKLREKAEEWRRLAEEATRYKHGSSVMNLETLIHKV 483

BLAST of Lsi03G008830 vs. TAIR 10
Match: AT1G22400.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 476.5 bits (1225), Expect = 2.5e-134
Identity = 219/482 (45.44%), Postives = 332/482 (68.88%), Query Frame = 0

Query: 3   NKVRAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGIL 62
           N  + HVVC+P P Q H+N M+ +AKLLH RGFY+TFV T Y H R +RSRG  +LDG+ 
Sbjct: 8   NSQKPHVVCVPYPAQGHINPMMRVAKLLHARGFYVTFVNTVYNHNRFLRSRGSNALDGLP 67

Query: 63  DFQFKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPSVT 122
            F+F++I D     +    +D  Q + +LC S   + LAPF  LL Q IN+ DN +P V+
Sbjct: 68  SFRFESIAD----GLPETDMDATQDITALCESTMKNCLAPFRELL-QRINAGDN-VPPVS 127

Query: 123 RIVADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK--------- 182
            IV+DG MSF ++   +  +P   F   S C+ L + HF   +++G+ P K         
Sbjct: 128 CIVSDGCMSFTLDVAEELGVPEVLFWTTSGCAFLAYLHFYLFIEKGLCPLKDESYLTKEY 187

Query: 183 ------DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDH 242
                 D+IP MKNV+LKD+PSFIRTT+P+D+ ++F +++ + + +AS +ILNTF+ L+H
Sbjct: 188 LEDTVIDFIPTMKNVKLKDIPSFIRTTNPDDVMISFALRETERAKRASAIILNTFDDLEH 247

Query: 243 DFLEALSHLFPPIYTVGPLHLFSTQIKDKTQE--EIATNLWEEQQECINWLDSQQPNSVL 302
           D + A+  + PP+Y+VGPLHL + +  ++  E   +++NLW+E+ EC++WLD++  NSV+
Sbjct: 248 DVVHAMQSILPPVYSVGPLHLLANREIEEGSEIGMMSSNLWKEEMECLDWLDTKTQNSVI 307

Query: 303 YVNFGSLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIG 362
           Y+NFGS+T+++++QL EFAWG+A S K FLW++RPD+V G+  ++P  F+ ETK+R M+ 
Sbjct: 308 YINFGSITVLSVKQLVEFAWGLAGSGKEFLWVIRPDLVAGEEAMVPPDFLMETKDRSMLA 367

Query: 363 SWCSQVEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIAL 422
           SWC Q +VL+HP+I GFLTH GWNS +ES+S GVPM+ WP+F DQQ  C++C   W + +
Sbjct: 368 SWCPQEKVLSHPAIGGFLTHCGWNSILESLSCGVPMVCWPFFADQQMNCKFCCDEWDVGI 427

Query: 423 EIENDVKRDEVESCIKELIEGKKGKEMKAKVIELRTKAE-ASYKPGGSSYLNFDQLITQI 467
           EI  DVKR+EVE+ ++EL++G+KGK+M+ K +E +  AE A+    GSS +NF+ ++++ 
Sbjct: 428 EIGGDVKREEVEAVVRELMDGEKGKKMREKAVEWQRLAEKATEHKLGSSVMNFETVVSKF 483

BLAST of Lsi03G008830 vs. TAIR 10
Match: AT1G22360.1 (UDP-glucosyl transferase 85A2 )

HSP 1 Score: 475.7 bits (1223), Expect = 4.3e-134
Identity = 221/478 (46.23%), Postives = 325/478 (67.99%), Query Frame = 0

Query: 6   RAHVVCIPVPLQSHVNAMLSLAKLLHQRGFYITFVLTEYTHQRIIRSRGPTSLDGILDFQ 65
           + HVVC+P P Q H+N M+ +AKLL+ +GF+ITFV T Y H R++RSRGP ++DG+  F+
Sbjct: 8   KQHVVCVPYPAQGHINPMMKVAKLLYAKGFHITFVNTVYNHNRLLRSRGPNAVDGLPSFR 67

Query: 66  FKTIWDCSMLSIDSNIIDTPQHLPSLCNSISNHFLAPFCTLLSQLINSNDNDIPSVTRIV 125
           F++I D     +    +D  Q +P+LC S   H LAPF  LL Q IN+ D D+P V+ IV
Sbjct: 68  FESIPD----GLPETDVDVTQDIPTLCESTMKHCLAPFKELLRQ-INARD-DVPPVSCIV 127

Query: 126 ADGFMSFCIEAGFKFNIPTAQFSPISACSLLGFFHFEELVQRGVTPFK------------ 185
           +DG MSF ++A  +  +P   F   SAC  L + ++   +++G++P K            
Sbjct: 128 SDGCMSFTLDAAEELGVPEVLFWTTSACGFLAYLYYYRFIEKGLSPIKDESYLTKEHLDT 187

Query: 186 --DWIPGMKNVRLKDLPSFIRTTDPNDIFLNFFIQQLKWSPKASCVILNTFEALDHDFLE 245
             DWIP MKN+RLKD+PSFIRTT+P+DI LNF I++   + +AS +ILNTF+ L+HD ++
Sbjct: 188 KIDWIPSMKNLRLKDIPSFIRTTNPDDIMLNFIIREADRAKRASAIILNTFDDLEHDVIQ 247

Query: 246 ALSHLFPPIYTVGPLHLFSTQIKDKTQE--EIATNLWEEQQECINWLDSQQPNSVLYVNF 305
           ++  + PP+Y++GPLHL   Q   +  E     +NLW E+ EC++WL+++  NSV+YVNF
Sbjct: 248 SMKSIVPPVYSIGPLHLLEKQESGEYSEIGRTGSNLWREETECLDWLNTKARNSVVYVNF 307

Query: 306 GSLTIMTLEQLKEFAWGIANSKKPFLWILRPDVVEGDSPILPQGFVEETKERGMIGSWCS 365
           GS+T+++ +QL EFAWG+A + K FLW++RPD+V GD  ++P  F+  T +R M+ SWC 
Sbjct: 308 GSITVLSAKQLVEFAWGLAATGKEFLWVIRPDLVAGDEAMVPPEFLTATADRRMLASWCP 367

Query: 366 QVEVLTHPSIKGFLTHNGWNSTIESISAGVPMISWPYFGDQQTTCRYCSVHWGIALEIEN 425
           Q +VL+HP+I GFLTH GWNST+ES+  GVPM+ WP+F +QQT C++    W + +EI  
Sbjct: 368 QEKVLSHPAIGGFLTHCGWNSTLESLCGGVPMVCWPFFAEQQTNCKFSRDEWEVGIEIGG 427

Query: 426 DVKRDEVESCIKELIEGKKGKEMKAKVIELRTKA-EASYKPGGSSYLNFDQLITQILL 467
           DVKR+EVE+ ++EL++ +KGK M+ K  E R  A EA+    GSS LNF+ L+ ++LL
Sbjct: 428 DVKREEVEAVVRELMDEEKGKNMREKAEEWRRLANEATEHKHGSSKLNFEMLVNKVLL 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F8WLS68.3e-15152.027-deoxyloganetin glucosyltransferase OS=Catharanthus roseus OX=4058 GN=UGT85A23 ... [more]
F8WKW12.4e-15052.117-deoxyloganetin glucosyltransferase OS=Gardenia jasminoides OX=114476 GN=UGT85A... [more]
Q6VAB32.5e-14752.10UDP-glycosyltransferase 85A8 OS=Stevia rebaudiana OX=55670 GN=UGT85A8 PE=2 SV=1[more]
B2XBQ51.0e-14049.17(R)-mandelonitrile beta-glucosyltransferase OS=Prunus dulcis OX=3755 GN=UGT85A19... [more]
Q9LMF01.1e-13948.41UDP-glycosyltransferase 85A5 OS=Arabidopsis thaliana OX=3702 GN=UGT85A5 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A1S3BXL82.1e-22178.13Glycosyltransferase OS=Cucumis melo OX=3656 GN=LOC103494320 PE=3 SV=1[more]
A0A0A0LY254.4e-21976.25Glycosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G687630 PE=3 SV=1[more]
A0A6J1D6V01.3e-19469.337-deoxyloganetin glucosyltransferase-like OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1DRI03.7e-19469.057-deoxyloganetin glucosyltransferase-like OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1JKN31.9e-18266.187-deoxyloganetin glucosyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_008453670.24.3e-22178.13PREDICTED: 7-deoxyloganetin glucosyltransferase-like [Cucumis melo][more]
KAE8653615.19.0e-21976.25hypothetical protein Csa_006847 [Cucumis sativus][more]
XP_004142536.19.0e-21976.257-deoxyloganetin glucosyltransferase [Cucumis sativus][more]
XP_022149077.12.6e-19469.337-deoxyloganetin glucosyltransferase-like [Momordica charantia][more]
XP_022156855.17.7e-19469.057-deoxyloganetin glucosyltransferase-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G22370.28.0e-14148.41UDP-glucosyl transferase 85A5 [more]
AT1G22380.13.9e-13545.13UDP-glucosyl transferase 85A3 [more]
AT1G22340.15.0e-13546.68UDP-glucosyl transferase 85A7 [more]
AT1G22400.12.5e-13445.44UDP-Glycosyltransferase superfamily protein [more]
AT1G22360.14.3e-13446.23UDP-glucosyl transferase 85A2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 8..451
e-value: 2.4E-143
score: 480.3
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 267..445
e-value: 2.4E-143
score: 480.3
NoneNo IPR availablePANTHERPTHR11926:SF1382PUTATIVE-RELATEDcoord: 5..464
NoneNo IPR availablePANTHERPTHR11926GLUCOSYL/GLUCURONOSYL TRANSFERASEScoord: 5..464
NoneNo IPR availableSUPERFAMILY53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 7..462
IPR002213UDP-glucuronosyl/UDP-glucosyltransferasePFAMPF00201UDPGTcoord: 203..431
e-value: 1.1E-25
score: 90.5
IPR002213UDP-glucuronosyl/UDP-glucosyltransferaseCDDcd03784GT1_Gtf-likecoord: 7..446
e-value: 1.45519E-78
score: 248.62

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G008830.1Lsi03G008830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0080043 quercetin 3-O-glucosyltransferase activity
molecular_function GO:0080044 quercetin 7-O-glucosyltransferase activity
molecular_function GO:0008194 UDP-glycosyltransferase activity