Lsi03G008740 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G008740
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionArginine and glutamate-rich protein 1
Locationchr03: 15054781 .. 15059602 (+)
RNA-Seq ExpressionLsi03G008740
SyntenyLsi03G008740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAACGTATTAAGGATAAATGAGAATGGACCAGAATAATTTTAGGGCGAAAAGGAATTGAACAGCAGACAGAGAAAGAAAGAAGGAAAGGGGTTGAAGACTCGAAGTTTGGTTCTTTCGGCTTTTGCCATTCCAAATCGGAGCGACGACGGAGACGGAAACAGGGAGAGGGCGGAGCGATATAATCCGCATCAACCCTTCTCCTTAATCCCCATCGCCGGAGATACCTTCTACCGGATTCTCCTCAATCTGAATTGGAACTTCATCAACTGCGATTTCTGGTAATATATACATGTATGCTTTCATGTCCGATCCTCTTTCTGCTTCTAATTAGGGTTTCGATTGCTCTTTATGCTACTACTGTCTCTATACTAGGGTTTTCGCTGCTTTACATGCAGCCCCTTTGTGGATATTTCCGTCCGTCTGCTTCCTGAATCAAGCGTCCGGTTTGACCTTTTCTAGTCGTTGATACTTTAGTTTTCTTGTTTGATGCTTTGTGGTTTATGGAAATGGAACAAGATGCTGACTCCTCAAATCCAATGTATTGATGTTTATTGATTGTTTATTGTATGGATTATGTTTATGTTAATCGAAGTAATTTGTTTACAGAGTGAAGATTTGTCCTTGTTGATACAAGATGCCTCGGGATTTATCACGGTCTCGATCACCTTCATATAGGCGGAGGCATTCACCATCTCCCTTAGGGCATAGGTATACCAGGAGAAGTCGAAGAGACAGGAGCCGTTCACCGTATTCATCGTACAGCAGGTGATCCCGTTTATGTTTTTAGTTTTATTTACTTTTGATTAATCGATTGAATAATCTCCAACGAATTATGAAAAGATTGACGACACCATATATTCCCACGCGGTTTGTATTTCGCTGACATCGCAGTTTCTGCATGAATCTTGTCCCTCTTGCTGTAAATTTCCACCTGTTTTTTCTTTCTAATATGTAAATTTCCCCCTTTTGCGGTTTTTTTGTATTTTAGTGTTGTTCTAAATTTACTGAACTTGTTCTGTGATGACTGATGAGGTAAGAGAACTTTGATTACACTAATTGAATGTTCATTTACAATTTCTTCTTATTATCATAATGTTGACAAGAAACAGTACGGGTATCTTTGTTGCTGTGTTTATTGGGTTTTCGTGTTCGTTGTGTTGTACAATTACATAAATGGATCCTTATAAGTTAGGGTTGCTTGTACTTCTCTTTCAGTCAGTCATGTCTTCCGGATCTATACGACCTCTTTTATTGTTTATTTGCTGATGAATATGATCTTCATGACCTCTTTTATTTATTGGCTGATGATTGGTTGTCTTGTTTTGTACGTGTGCTTTACTATTATGAATTTCACATAAAATTTCTGATTTTTTTGGCATAATTGTCAAGTACCTCCTGATGGTATTTAATCATTTGTTGAGATTGATGAGTGGGGGAGAAAATTTTAAGTCTTCGGGGGGAAAAACTCTAAAATGTGGTGAGAAGCAAACAGAATAGACATGATCTTGGTAAGAAAATTGTGAAATAAGTCAGTTCTTCCTATTACATGGGAAGTGACCAGGTTAAGGGTGCTGATGGGTGTCCTGTCATTGCTCGATTGTGACTTTGCTTCTTCATGTGATGTGATAAAAGATGGAGCTCACAAAGGTTTATTCTTAACAATGTATGAGGTAACTGTACGGTGCAAAGTGGTAGGCTAGCTTGATATATACCTATGTGTAATAATGTAATGAAGTCTTTATCTCTTGCTCAAATATTTCTATTCATTATACAGAAGAAAAAGCCGCTCAATTTCTCCAAGACGTAATAGAAGTCGTTCACGAACACCAAGACGCCATAGAAGTCGTTCTCCAATTTCAAGGAGTTACAAGAAGCAAAGACGGCGGAGTTCCTCATCATCTCTACATCGTAGATCTTCTAGTTCTAGCCTTGGATCCATTGAGCAAAAAAGTACCAGTGAAAAATTGAAAAAGGAGGAGGAAGAAAGAAAAAGGTATATTAACTGGCTCACTGATTTTATGTTCTTTTTTCTGCCTCTCAATTGTCCTTGGGTTCATTGCAGCATCATCTAATATTCTATAATGTAAAATGTTGTCACTTGTAGACTTTTGTAGTTCGTTTAAGTACTTTGCTGGCATTAACCTTTTGAACCATGAAAGGTTATGGCATAACTAAAATCCACATTAGGATGACAGTGAATTCTTGCCCTAAGTATCACTATTAGTGTTATCTTTAAACAAGAGTCAAAATAATGATTACCCTTTAGCTGGAAAGGGATTTAAAAGCAGAATCAGGATAATAATTGTTTACATGGAGAATCTTTAATTATTTTTTTTTCCAAATTTGAAACGTTTTCCTTTTTTGACTGGAAGGACATGGGTTGATACTTATTTTTAGCGGGTTCTTTTGTTAACTCATAAAACGAGGAATGGTCAGTTCCACACTCCAGCTGGTCACCATGTGTGTAATTCTGAAACACTAAAAATCTAATTGAGCTTGAGTTTAATAAGCTGTTCCAGAAATGCTGTCAGTTGTGCATATGCTAGAACTTTGTATGTACTTGCATCTGCAGTGCATTGCTTGTTCTTGCGAAGATTCCTTAAGATAATGAAACTATAGGCTCTAGGTGGATGATAATTCAATGTATTTGGAATTGCACCTCTAAAGATTATGATTTTGTGTGAGCACATTCTTTTTTAATTTCACATTATAAAGGTCTAGAACTGATTTTCTTTTAGTATTGGAACTGATTGAAATTGACTACAGATTTGTTTAATAATGTTACAATTCTGTGATATCATCTCCCTTTCAGTTTTCTCATTGCAGCGTTCTCATTGTTTTTATTGTTAATGAGAGCTATATATGTGTTATAGATGATCAAAAACAATATCTGTAAACTTATCATACGTGGATGTTGCATTCTCATGCTCGTTTATATGGTGTGCTGAAAATATTTATTATTTGATTGGTTTGGAACATCAAAGGAAGGTGTACCACGTTTTATTTATTTAATTTACGAGGCACCTTAATTTTAGGTGGTAAGGGTATAAAATGACTCAAGTGAAAACCTAATAGTGGGATGTAGGCATTGTTTTGCTCACCAGAACCCCCTTTTTTTCCTTTTAATTTCAATTGTATGTACGGAATTGGAGAAATCTATATACTCTTTTGGAGTCTGAGGAGTCTAATAGTTTGATGCTTCAGAATCTGCTCCTTTTATTTCTGCAATGTGCTTCTGCTATGTGGAATTTTTATCCCATCTCTGACTTTTGCCCTTCATTCTAGTTCGTTCTTATAATATTGTTGGTATTGGATTGACTGGCTAATTTATATTCCTGTAAATTCTCAGACTTCTATGTCTCAGTAGTTCTCTATGACTGTTGCGCTTGCTAATGAACCTTATCCCTAACTGATTTAAATTTGAGCAAGTGGTTTTAAAATGGGAGGAACTAATGTAATATGTGGCTTATTCAATTACTAGGTTGTTGTGAATAACAGTCTGCTGTTATCATGCATGCCTATGTGAATGTCTAATATGAAATATTTGTACAGTCCGTCACGTGTGAAGTTACACTCTCAGGATGACAGTATTTGTTGACTAGTTTGAGTTTAATAGGCCTCTAAGAACCTCATTTTTACATGAAATCCCTACTGTTTTAGTTTAAAGGGTCGGTCATAACTGCAAATGCAAAAATGGAAGATACTTTTCACCATTTTGATGTTCTAGAGCTACATGGATAAAAATTTCATTTTTTACATAAGAAAAGAAAAAGTAATATACTCCCCATTCAAAGAATGTTATAGTGTAAGGATTTGAGTTCTTCCAGTGATCGGTTTTAAAACTGCGACAGGTTACTTGACATTCTTCTCCACAGGATTTTCTGAAGGAAGATACTGCTATAGACTTGTTATATTTGATGATCAATTTGGTGCTTCGTTAATATGCTGTTCACCAGTTTTGTAACTCTTTTGTATGGATCAGGATTGAGTTAGAGGTATGCCCTAAAAGGATAAGCATGTTTTAGTGCTCTTATCGTCATGGAGGTTAGTTTGTTAATTTACTATATCCTTATATAAACCGGAAGGGGAAAAAACAACGGGCCGAGGCATGATTAAAGTCAGTTGTCATTTAATTGTTGTTTGAATTGGAAGGACGCTTCAGGTGGTGCATTTTTCACATATATCCGTGGTTAGCTGTTTTCTTAAATTTTTATTTTTCTCTCAGGCGTCAACAGGAGACAGAGGCCAAATTGCTAAAAGAAGAAACAACAAAGAGAGTGGAAGAAGCAATTTGCAAGGAAGTTGAACAGAGCCTGAACTCCGATGATTTAAAACTGGAAATAGTCAAGAAGTTGGAGGAGGGACGGAGACGGCTTAACGAAGAAGTGAGTGCTCAACTTGAGAAGGAAAAGGAAGCTGCCCTTGTTGAGGCCAGACGGAAAGAGGTACGACTAATATTTATGAGTACTATGGCTTAACGGTTTGGTTTGGTTCGGTTAAAGTTTGGATAGATGTTTAAGAAATGTGGGTTTGGTTTTTGTAGGAAGAAGCTAGAAAAGAGAAAGAAGAGGTAGAAAGAATGGTTGAGGAGAATCGGAGGAGAGTAGAAGAGGCTCAGAGAAGAGAGGCTTTAGAGAGACAAAAGAGAGAAGAGGAAAGATATAGAGAACTGGAAGAGCTACAAAGGCAAAAAGAAGAAGCTATTAAGAGGAAAAAACAGGAAGAAGAGGAACAGAGAGTTAATCAGATGAAACTGTTGGGTAAAAACAAATCACGTCCTAAATTGTCATTTGCCATTGGCTCCAAATAAAAAAAA

mRNA sequence

AAAAAAAACGTATTAAGGATAAATGAGAATGGACCAGAATAATTTTAGGGCGAAAAGGAATTGAACAGCAGACAGAGAAAGAAAGAAGGAAAGGGGTTGAAGACTCGAAGTTTGGTTCTTTCGGCTTTTGCCATTCCAAATCGGAGCGACGACGGAGACGGAAACAGGGAGAGGGCGGAGCGATATAATCCGCATCAACCCTTCTCCTTAATCCCCATCGCCGGAGATACCTTCTACCGGATTCTCCTCAATCTGAATTGGAACTTCATCAACTGCGATTTCTGGTAATATATACATGTATGCTTTCATGTCCGATCCTCTTTCTGCTTCTAATTAGGGTTTCGATTGCTCTTTATGCTACTACTGTCTCTATACTAGGGTTTTCGCTGCTTTACATGCAGCCCCTTTGTGGATATTTCCGTCCGTCTGCTTCCTGAATCAAGCGTCCGGTTTGACCTTTTCTAGTCGTTGATACTTTAGTTTTCTTGTTTGATGCTTTGTGGTTTATGGAAATGGAACAAGATGCTGACTCCTCAAATCCAATAGTGAAGATTTGTCCTTGTTGATACAAGATGCCTCGGGATTTATCACGGTCTCGATCACCTTCATATAGGCGGAGGCATTCACCATCTCCCTTAGGGCATAGGTATACCAGGAGAAGTCGAAGAGACAGGAGCCGTTCACCGTATTCATCGTACAGCAGGTTAAGGGTGCTGATGGGTGTCCTGTCATTGCTCGATTGTGACTTTGCTTCTTCATGTGATGTGATAAAAGATGGAGCTCACAAAGAAGAAAAAGCCGCTCAATTTCTCCAAGACGTAATAGAAGTCGTTCACGAACACCAAGACGCCATAGAAGTCGTTCTCCAATTTCAAGGAGTTACAAGAAGCAAAGACGGCGGAGTTCCTCATCATCTCTACATCGTAGATCTTCTAGTTCTAGCCTTGGATCCATTGAGCAAAAAACGGGTTCTTTTGTTAACTCATAAAACGAGGAATGGTCAGTTCCACACTCCAGCTGGTCACCATGTGTTGGGATGTAGGCATTGTTTTGCTCACCAGAACCCCCTTTTTTTCCTTTTAATTTCAATTTTCGTTCTTATAATATTGTTGGTTGTTGTGAATAACAGTCTGCTGTTATCATGCATGCCTATTTTAAAGGGTTACTTGACATTCTTCTCCACAGGATTTTCTGAAGGAAGATACTGCTATAGACTTGTTATATTTGATGATCAATTTGGTGCTTCGTTAATATGCTGTTCACCAGTTTTGCGTCAACAGGAGACAGAGGCCAAATTGCTAAAAGAAGAAACAACAAAGAGAGTGGAAGAAGCAATTTGCAAGGAAGTTGAACAGAGCCTGAACTCCGATGATTTAAAACTGGAAATAGTCAAGAAGTTGGAGGAGGGACGGAGACGGCTTAACGAAGAAGTGAGTGCTCAACTTGAGAAGGAAAAGGAAGCTGCCCTTGTTGAGGCCAGACGGAAAGAGGAAGAAGCTAGAAAAGAGAAAGAAGAGGTAGAAAGAATGGTTGAGGAGAATCGGAGGAGAGTAGAAGAGGCTCAGAGAAGAGAGGCTTTAGAGAGACAAAAGAGAGAAGAGGAAAGATATAGAGAACTGGAAGAGCTACAAAGGCAAAAAGAAGAAGCTATTAAGAGGAAAAAACAGGAAGAAGAGGAACAGAGAGTTAATCAGATGAAACTGTTGGGTAAAAACAAATCACGTCCTAAATTGTCATTTGCCATTGGCTCCAAATAAAAAAAA

Coding sequence (CDS)

ATGCCTCGGGATTTATCACGGTCTCGATCACCTTCATATAGGCGGAGGCATTCACCATCTCCCTTAGGGCATAGGTATACCAGGAGAAGTCGAAGAGACAGGAGCCGTTCACCGTATTCATCGTACAGCAGGTTAAGGGTGCTGATGGGTGTCCTGTCATTGCTCGATTGTGACTTTGCTTCTTCATGTGATGTGATAAAAGATGGAGCTCACAAAGAAGAAAAAGCCGCTCAATTTCTCCAAGACGTAATAGAAGTCGTTCACGAACACCAAGACGCCATAGAAGTCGTTCTCCAATTTCAAGGAGTTACAAGAAGCAAAGACGGCGGAGTTCCTCATCATCTCTACATCGTAGATCTTCTAGTTCTAGCCTTGGATCCATTGAGCAAAAAACGGGTTCTTTTGTTAACTCATAAAACGAGGAATGGTCAGTTCCACACTCCAGCTGGTCACCATGTGTTGGGATGTAGGCATTGTTTTGCTCACCAGAACCCCCTTTTTTTCCTTTTAATTTCAATTTTCGTTCTTATAATATTGTTGGTTGTTGTGAATAACAGTCTGCTGTTATCATGCATGCCTATTTTAAAGGGTTACTTGACATTCTTCTCCACAGGATTTTCTGAAGGAAGATACTGCTATAGACTTGTTATATTTGATGATCAATTTGGTGCTTCGTTAATATGCTGTTCACCAGTTTTGCGTCAACAGGAGACAGAGGCCAAATTGCTAAAAGAAGAAACAACAAAGAGAGTGGAAGAAGCAATTTGCAAGGAAGTTGAACAGAGCCTGAACTCCGATGATTTAAAACTGGAAATAGTCAAGAAGTTGGAGGAGGGACGGAGACGGCTTAACGAAGAAGTGAGTGCTCAACTTGAGAAGGAAAAGGAAGCTGCCCTTGTTGAGGCCAGACGGAAAGAGGAAGAAGCTAGAAAAGAGAAAGAAGAGGTAGAAAGAATGGTTGAGGAGAATCGGAGGAGAGTAGAAGAGGCTCAGAGAAGAGAGGCTTTAGAGAGACAAAAGAGAGAAGAGGAAAGATATAGAGAACTGGAAGAGCTACAAAGGCAAAAAGAAGAAGCTATTAAGAGGAAAAAACAGGAAGAAGAGGAACAGAGAGTTAATCAGATGAAACTGTTGGGTAAAAACAAATCACGTCCTAAATTGTCATTTGCCATTGGCTCCAAATAA

Protein sequence

MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLDCDFASSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLYIVDLLVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLGCRHCFAHQNPLFFLLISIFVLIILLVVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQETEAKLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKEAALVEARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEAIKRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK
Homology
BLAST of Lsi03G008740 vs. ExPASy Swiss-Prot
Match: P0CB26 (Uncharacterized protein At1g10890 OS=Arabidopsis thaliana OX=3702 GN=At1g10890 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.5e-44
Identity = 166/398 (41.71%), Postives = 211/398 (53.02%), Query Frame = 0

Query: 1   MPRDLSRSR----SPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLD 60
           MPRDLSRSR    SPS RR+HS SP+  R++RRSRRDRS SPYSS+S  R          
Sbjct: 1   MPRDLSRSRSPSPSPSRRRKHSRSPVRQRHSRRSRRDRSPSPYSSHSYSR---------- 60

Query: 61  CDFASSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLY 120
                    I    H+                            + VT  +    P    
Sbjct: 61  ----RKSRSISPRRHRS---------------------------RSVTPKRRSPTP---- 120

Query: 121 IVDLLVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLGCRHCFAHQNPLFFLLISIFVL 180
                         KR     +K +  +  TP+           A ++P           
Sbjct: 121 --------------KR-----YKRQKSRSSTPSP----------AKRSPA---------- 180

Query: 181 IILLVVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQ 240
                                  T  S     G    R    +++            RQ+
Sbjct: 181 ----------------------ATLESAKNRNGEKLKR----EEE--------ERKRRQR 240

Query: 241 ETEAKLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKE 300
           E E KL++EET KRVEEAI K+VE+SL S+ +K+EI+  LEEGR+RLNEEV+AQLE+EKE
Sbjct: 241 EAELKLIEEETVKRVEEAIRKKVEESLQSEKIKMEILTLLEEGRKRLNEEVAAQLEEEKE 280

Query: 301 AALVEARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQK 360
           A+L+EA+ KEE  ++EKEE ER+ EEN +RVEEAQR+EA+ERQ++EEERYRELEELQRQK
Sbjct: 301 ASLIEAKEKEEREQQEKEERERIAEENLKRVEEAQRKEAMERQRKEEERYRELEELQRQK 280

Query: 361 EEAIKRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 395
           EEA++RKK EEEE+R+ QMKLLGKNKSRPKLSFA+ SK
Sbjct: 361 EEAMRRKKAEEEEERLKQMKLLGKNKSRPKLSFALSSK 280

BLAST of Lsi03G008740 vs. ExPASy Swiss-Prot
Match: Q2TA42 (Arginine and glutamate-rich protein 1 OS=Bos taurus OX=9913 GN=ARGLU1 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.1e-15
Identity = 64/161 (39.75%), Postives = 112/161 (69.57%), Query Frame = 0

Query: 233 LRQQETEAKLLKEETTKRVEEAICKEVEQSL--NSDDLKLEIVKKLEEGRRRLNEEVSAQ 292
           +RQQE E KL++EET +RVEE + K VE+ L    D+++ E+++++EE +R + +++  +
Sbjct: 116 IRQQEIEEKLIEEETARRVEELVAKRVEEELEKRKDEIEREVLRRVEEAKRIMEKQLLEE 175

Query: 293 LEKEKEAALVEARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELE 352
           LE++++A L   + +EEE R ++EE+ER++EEN R++ EAQ + A       EE+ R +E
Sbjct: 176 LERQRQAELAAQKAREEEERAKREELERILEENNRKIAEAQAKLA-------EEQLRIVE 235

Query: 353 ELQRQKEEAIKRKKQEEEEQRVNQMKLLGKNKSRPKLSFAI 392
           E ++  EE +K +++ + +Q+  Q  +LGK KSRPKLSF++
Sbjct: 236 EQRKIHEERMKLEQERQRQQKEEQKIILGKGKSRPKLSFSL 269

BLAST of Lsi03G008740 vs. ExPASy Swiss-Prot
Match: Q9NWB6 (Arginine and glutamate-rich protein 1 OS=Homo sapiens OX=9606 GN=ARGLU1 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.1e-15
Identity = 64/161 (39.75%), Postives = 112/161 (69.57%), Query Frame = 0

Query: 233 LRQQETEAKLLKEETTKRVEEAICKEVEQSL--NSDDLKLEIVKKLEEGRRRLNEEVSAQ 292
           +RQQE E KL++EET +RVEE + K VE+ L    D+++ E+++++EE +R + +++  +
Sbjct: 116 IRQQEIEEKLIEEETARRVEELVAKRVEEELEKRKDEIEREVLRRVEEAKRIMEKQLLEE 175

Query: 293 LEKEKEAALVEARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELE 352
           LE++++A L   + +EEE R ++EE+ER++EEN R++ EAQ + A       EE+ R +E
Sbjct: 176 LERQRQAELAAQKAREEEERAKREELERILEENNRKIAEAQAKLA-------EEQLRIVE 235

Query: 353 ELQRQKEEAIKRKKQEEEEQRVNQMKLLGKNKSRPKLSFAI 392
           E ++  EE +K +++ + +Q+  Q  +LGK KSRPKLSF++
Sbjct: 236 EQRKIHEERMKLEQERQRQQKEEQKIILGKGKSRPKLSFSL 269

BLAST of Lsi03G008740 vs. ExPASy Swiss-Prot
Match: Q3UL36 (Arginine and glutamate-rich protein 1 OS=Mus musculus OX=10090 GN=Arglu1 PE=1 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 1.1e-15
Identity = 64/161 (39.75%), Postives = 112/161 (69.57%), Query Frame = 0

Query: 233 LRQQETEAKLLKEETTKRVEEAICKEVEQSL--NSDDLKLEIVKKLEEGRRRLNEEVSAQ 292
           +RQQE E KL++EET +RVEE + K VE+ L    D+++ E+++++EE +R + +++  +
Sbjct: 114 IRQQEIEEKLIEEETARRVEELVAKRVEEELEKRKDEIEREVLRRVEEAKRIMEKQLLEE 173

Query: 293 LEKEKEAALVEARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELE 352
           LE++++A L   + +EEE R ++EE+ER++EEN R++ EAQ + A       EE+ R +E
Sbjct: 174 LERQRQAELAAQKAREEEERAKREELERILEENNRKIAEAQAKLA-------EEQLRIVE 233

Query: 353 ELQRQKEEAIKRKKQEEEEQRVNQMKLLGKNKSRPKLSFAI 392
           E ++  EE +K +++ + +Q+  Q  +LGK KSRPKLSF++
Sbjct: 234 EQRKIHEERMKLEQERQRQQKEEQKIILGKGKSRPKLSFSL 267

BLAST of Lsi03G008740 vs. ExPASy Swiss-Prot
Match: Q5BJT0 (Arginine and glutamate-rich protein 1 OS=Rattus norvegicus OX=10116 GN=Arglu1 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.1e-15
Identity = 64/161 (39.75%), Postives = 112/161 (69.57%), Query Frame = 0

Query: 233 LRQQETEAKLLKEETTKRVEEAICKEVEQSL--NSDDLKLEIVKKLEEGRRRLNEEVSAQ 292
           +RQQE E KL++EET +RVEE + K VE+ L    D+++ E+++++EE +R + +++  +
Sbjct: 114 IRQQEIEEKLIEEETARRVEELVAKRVEEELEKRKDEIEREVLRRVEEAKRIMEKQLLEE 173

Query: 293 LEKEKEAALVEARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELE 352
           LE++++A L   + +EEE R ++EE+ER++EEN R++ EAQ + A       EE+ R +E
Sbjct: 174 LERQRQAELAAQKAREEEERAKREELERILEENNRKIAEAQAKLA-------EEQLRIVE 233

Query: 353 ELQRQKEEAIKRKKQEEEEQRVNQMKLLGKNKSRPKLSFAI 392
           E ++  EE +K +++ + +Q+  Q  +LGK KSRPKLSF++
Sbjct: 234 EQRKIHEERMKLEQERQRQQKEEQKIILGKGKSRPKLSFSL 267

BLAST of Lsi03G008740 vs. ExPASy TrEMBL
Match: A0A6J1EIS1 (uncharacterized protein At1g10890-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434532 PE=4 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 3.9e-80
Identity = 222/394 (56.35%), Postives = 251/394 (63.71%), Query Frame = 0

Query: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLDCDFA 60
           MPRDLSRSRSPSYRRRHSPSPLGHRY+RRSRRDRSRSPYSSYSRL VLMGVLSLLDCDFA
Sbjct: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYSRRSRRDRSRSPYSSYSRLWVLMGVLSLLDCDFA 60

Query: 61  SSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLYIVDL 120
           SSCDVIKDGAHK                           F  + RS+     HH      
Sbjct: 61  SSCDVIKDGAHKG-------------------------LFLTIRRSRSISPRHH------ 120

Query: 121 LVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLGCRHCFAHQNPLFFLLISIFVLIILL 180
                   S+ R    T +    +  T   +     R   +  +P               
Sbjct: 121 -------RSRSR----TPRRHRSRSPTSRSYRKQRRRSSSSSLHPR-------------- 180

Query: 181 VVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQETEA 240
                                 S+G S G    +     ++F           RQ+ TEA
Sbjct: 181 ----------------------SSGSSPGSIEQKST--SEKFRKEEE--ERKRRQKVTEA 240

Query: 241 KLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKEAALV 300
           K L++ET KR+EEAI K+VE SLN DD+K+EI +K+EEGRRRLNEEV+AQLEKEKEAAL+
Sbjct: 241 KFLEKETAKRLEEAIRKKVEASLNDDDVKVEINRKMEEGRRRLNEEVAAQLEKEKEAALI 300

Query: 301 EARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEAI 360
           EA+RKEEEAR+EKEEVERMVEE RRRVEEAQR EALERQ+REEERYRELEELQR+KEEAI
Sbjct: 301 EAQRKEEEARREKEEVERMVEERRRRVEEAQRSEALERQQREEERYRELEELQRKKEEAI 312

Query: 361 KRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 395
           KRKKQEEEEQR+NQMKLLGKNKSRPKLSFAIGSK
Sbjct: 361 KRKKQEEEEQRLNQMKLLGKNKSRPKLSFAIGSK 312

BLAST of Lsi03G008740 vs. ExPASy TrEMBL
Match: A0A6J1JQ20 (uncharacterized protein At1g10890-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486696 PE=4 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 5.3e-77
Identity = 222/395 (56.20%), Postives = 251/395 (63.54%), Query Frame = 0

Query: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLDCDFA 60
           MPRDLSRSRSPSYRRR SPSPLGHRY+RRSRRDRSRSPYSSYSRL VLMGVLSLLDCDFA
Sbjct: 1   MPRDLSRSRSPSYRRRLSPSPLGHRYSRRSRRDRSRSPYSSYSRLWVLMGVLSLLDCDFA 60

Query: 61  SSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLYIVDL 120
           SSCDVIKDGAHK                           F  + +S+             
Sbjct: 61  SSCDVIKDGAHKG-------------------------LFLTIRKSRS------------ 120

Query: 121 LVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLG-CRHCFAHQNPLFFLLISIFVLIIL 180
                  +S +R     H++R+    TP  H         +  Q                
Sbjct: 121 -------ISPRR-----HRSRS---RTPRRHRSRSPTSRSYRKQR--------------- 180

Query: 181 LVVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQETE 240
                 S   S  P   G     S G  E +        +++            RQ+ TE
Sbjct: 181 ----RRSSSSSLHPRSSG----SSPGSIEQKSTSEKFRKEEE--------ERKRRQKVTE 240

Query: 241 AKLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKEAAL 300
           AK L++ET KR+EEAI K+VE SLN +D+KL I +K+EEGRRRLNEEV+AQLEKEKEAAL
Sbjct: 241 AKFLEKETAKRLEEAIRKKVEASLNDEDVKLGINRKMEEGRRRLNEEVTAQLEKEKEAAL 300

Query: 301 VEARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEA 360
           +EA+RKEEEARKEKEEVERMVEE RRRVEEAQR EALERQ+REEERYRELEELQR+KEEA
Sbjct: 301 IEAQRKEEEARKEKEEVERMVEERRRRVEEAQRSEALERQQREEERYRELEELQRKKEEA 312

Query: 361 IKRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 395
           IKRKKQEEEEQR+NQMKLLGKNKSRPKLSFAIGSK
Sbjct: 361 IKRKKQEEEEQRLNQMKLLGKNKSRPKLSFAIGSK 312

BLAST of Lsi03G008740 vs. ExPASy TrEMBL
Match: A0A0A0KEG0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G411270 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 1.4e-61
Identity = 199/394 (50.51%), Postives = 224/394 (56.85%), Query Frame = 0

Query: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLDCDFA 60
           MPRDLSRSRSP YRRR SPSP+GHRYTRRSRRDRSRSPYSSYSR +              
Sbjct: 1   MPRDLSRSRSPLYRRRLSPSPVGHRYTRRSRRDRSRSPYSSYSRRK-------------- 60

Query: 61  SSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLYIVDL 120
                                                      +RS              
Sbjct: 61  -------------------------------------------SRS-------------- 120

Query: 121 LVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLGCRHCFAHQNPLFFLLISIFVLIILL 180
                  +S +R    +   R+ +  +P        R                       
Sbjct: 121 -------ISPRRNRSRSRTPRHHRSRSPTSRSYKKQRR---------------------- 180

Query: 181 VVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQETEA 240
                    S    L    +  S G  E +     +  +++            RQQET+ 
Sbjct: 181 --------RSSSSSLHRRSSSSSLGSIEQKSTSEKLKKEEE---------RKRRQQETQG 240

Query: 241 KLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKEAALV 300
           KLLKEETTKRVE+AI KEVE+ LNSDD+KL+I KKLEEGR RLNEEV+AQLEKEKEAALV
Sbjct: 241 KLLKEETTKRVEDAIRKEVEERLNSDDVKLDINKKLEEGRTRLNEEVTAQLEKEKEAALV 277

Query: 301 EARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEAI 360
           EARR+EE+ARKEKEE+ERMVEE+RRRVEEAQRREALERQKREEERYRELEELQRQKEEAI
Sbjct: 301 EARRREEQARKEKEELERMVEESRRRVEEAQRREALERQKREEERYRELEELQRQKEEAI 277

Query: 361 KRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 395
           KRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK
Sbjct: 361 KRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 277

BLAST of Lsi03G008740 vs. ExPASy TrEMBL
Match: A0A5A7UT49 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold496G00230 PE=4 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 1.6e-60
Identity = 201/394 (51.02%), Postives = 224/394 (56.85%), Query Frame = 0

Query: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLDCDFA 60
           MPRDLSRSRSP YRRR SPSPLGHRY+RRSRRDRSRSPY SYSR +              
Sbjct: 1   MPRDLSRSRSPLYRRRLSPSPLGHRYSRRSRRDRSRSPY-SYSRRK-------------- 60

Query: 61  SSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLYIVDL 120
                                                      +RS              
Sbjct: 61  -------------------------------------------SRS-------------- 120

Query: 121 LVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLGCRHCFAHQNPLFFLLISIFVLIILL 180
                  +S +R    +   R+ +  +P        R                       
Sbjct: 121 -------ISPRRNRSRSRTPRHHRSRSPTSRSYKKQRR---------------------- 180

Query: 181 VVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQETEA 240
                    S    L    +  S G  E +     +  +++            RQQET+A
Sbjct: 181 --------RSSSSSLHRRSSSSSLGSIEQKSTSEKLKKEEE---------RKRRQQETQA 240

Query: 241 KLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKEAALV 300
           KLLKEETTKRVEEAI KEVE+ LNSD+LKL+I KKLEEGR RLNEEV+AQLEKEKEAALV
Sbjct: 241 KLLKEETTKRVEEAIRKEVEERLNSDNLKLDINKKLEEGRTRLNEEVTAQLEKEKEAALV 276

Query: 301 EARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEAI 360
           EARRKEE+ARKEKEE+ERMVEE+RRRVEEAQRREALERQKREEERYRELEELQRQKEEAI
Sbjct: 301 EARRKEEQARKEKEELERMVEESRRRVEEAQRREALERQKREEERYRELEELQRQKEEAI 276

Query: 361 KRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 395
           KRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK
Sbjct: 361 KRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 276

BLAST of Lsi03G008740 vs. ExPASy TrEMBL
Match: A0A6J1EIF0 (uncharacterized protein At1g10890-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434532 PE=4 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.0e-59
Identity = 197/395 (49.87%), Postives = 223/395 (56.46%), Query Frame = 0

Query: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLDCDFA 60
           MPRDLSRSRSPSYRRRHSPSPLGHRY+RRSRRDRSRSPYSSYSR R              
Sbjct: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYSRRSRRDRSRSPYSSYSRRR-------------- 60

Query: 61  SSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLYIVDL 120
                                                      +RS     P H      
Sbjct: 61  -------------------------------------------SRSIS---PRH------ 120

Query: 121 LVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLG-CRHCFAHQNPLFFLLISIFVLIIL 180
                            H++R+    TP  H         +  Q                
Sbjct: 121 -----------------HRSRS---RTPRRHRSRSPTSRSYRKQR--------------- 180

Query: 181 LVVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQETE 240
                 S   S  P   G     S G  E +        +++            RQ+ TE
Sbjct: 181 ----RRSSSSSLHPRSSG----SSPGSIEQKSTSEKFRKEEE--------ERKRRQKVTE 240

Query: 241 AKLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKEAAL 300
           AK L++ET KR+EEAI K+VE SLN DD+K+EI +K+EEGRRRLNEEV+AQLEKEKEAAL
Sbjct: 241 AKFLEKETAKRLEEAIRKKVEASLNDDDVKVEINRKMEEGRRRLNEEVAAQLEKEKEAAL 278

Query: 301 VEARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEA 360
           +EA+RKEEEAR+EKEEVERMVEE RRRVEEAQR EALERQ+REEERYRELEELQR+KEEA
Sbjct: 301 IEAQRKEEEARREKEEVERMVEERRRRVEEAQRSEALERQQREEERYRELEELQRKKEEA 278

Query: 361 IKRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 395
           IKRKKQEEEEQR+NQMKLLGKNKSRPKLSFAIGSK
Sbjct: 361 IKRKKQEEEEQRLNQMKLLGKNKSRPKLSFAIGSK 278

BLAST of Lsi03G008740 vs. NCBI nr
Match: XP_038888588.1 (uncharacterized protein At1g10890 isoform X3 [Benincasa hispida])

HSP 1 Score: 322.8 bits (826), Expect = 4.2e-84
Identity = 233/394 (59.14%), Postives = 254/394 (64.47%), Query Frame = 0

Query: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLDCDFA 60
           MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRV M VLSLLDCDFA
Sbjct: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVPMVVLSLLDCDFA 60

Query: 61  SSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLYIVDL 120
           SSCDVIKDGAHK                                          L+    
Sbjct: 61  SSCDVIKDGAHK-----------------------------------------GLF---- 120

Query: 121 LVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLGCRHCFAHQNPLFFLLISIFVLIILL 180
                  L+ ++   ++ +    +  TP  H     R   +                   
Sbjct: 121 -------LTIRKSCSISPRRNRSRSRTPRRHR---SRSSISRS----------------- 180

Query: 181 VVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQETEA 240
                    S    L    +  S G  E +     +  +++            RQQETEA
Sbjct: 181 --YKKQRRRSSSSSLHRRSSSSSLGSIEQKSTSEKLKKEEE--------ERKRRQQETEA 240

Query: 241 KLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKEAALV 300
           KLLKEET KRVEEAI KEVEQSLNS+DLK+EI KKLEEGR+RLNEEV+AQLEKEKEAALV
Sbjct: 241 KLLKEETAKRVEEAIRKEVEQSLNSNDLKVEIDKKLEEGRKRLNEEVTAQLEKEKEAALV 300

Query: 301 EARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEAI 360
           EARRKEE+ARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEAI
Sbjct: 301 EARRKEEQARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEAI 312

Query: 361 KRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 395
           KRKKQEEEEQR+NQMKLLGKNKSRPKLSFAIGSK
Sbjct: 361 KRKKQEEEEQRINQMKLLGKNKSRPKLSFAIGSK 312

BLAST of Lsi03G008740 vs. NCBI nr
Match: XP_022927714.1 (uncharacterized protein At1g10890-like isoform X1 [Cucurbita moschata] >XP_022927715.1 uncharacterized protein At1g10890-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 308.5 bits (789), Expect = 8.1e-80
Identity = 222/394 (56.35%), Postives = 251/394 (63.71%), Query Frame = 0

Query: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLDCDFA 60
           MPRDLSRSRSPSYRRRHSPSPLGHRY+RRSRRDRSRSPYSSYSRL VLMGVLSLLDCDFA
Sbjct: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYSRRSRRDRSRSPYSSYSRLWVLMGVLSLLDCDFA 60

Query: 61  SSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLYIVDL 120
           SSCDVIKDGAHK                           F  + RS+     HH      
Sbjct: 61  SSCDVIKDGAHKG-------------------------LFLTIRRSRSISPRHH------ 120

Query: 121 LVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLGCRHCFAHQNPLFFLLISIFVLIILL 180
                   S+ R    T +    +  T   +     R   +  +P               
Sbjct: 121 -------RSRSR----TPRRHRSRSPTSRSYRKQRRRSSSSSLHPR-------------- 180

Query: 181 VVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQETEA 240
                                 S+G S G    +     ++F           RQ+ TEA
Sbjct: 181 ----------------------SSGSSPGSIEQKST--SEKFRKEEE--ERKRRQKVTEA 240

Query: 241 KLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKEAALV 300
           K L++ET KR+EEAI K+VE SLN DD+K+EI +K+EEGRRRLNEEV+AQLEKEKEAAL+
Sbjct: 241 KFLEKETAKRLEEAIRKKVEASLNDDDVKVEINRKMEEGRRRLNEEVAAQLEKEKEAALI 300

Query: 301 EARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEAI 360
           EA+RKEEEAR+EKEEVERMVEE RRRVEEAQR EALERQ+REEERYRELEELQR+KEEAI
Sbjct: 301 EAQRKEEEARREKEEVERMVEERRRRVEEAQRSEALERQQREEERYRELEELQRKKEEAI 312

Query: 361 KRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 395
           KRKKQEEEEQR+NQMKLLGKNKSRPKLSFAIGSK
Sbjct: 361 KRKKQEEEEQRLNQMKLLGKNKSRPKLSFAIGSK 312

BLAST of Lsi03G008740 vs. NCBI nr
Match: XP_031742559.1 (uncharacterized protein At1g10890 isoform X1 [Cucumis sativus] >XP_031742560.1 uncharacterized protein At1g10890 isoform X1 [Cucumis sativus] >XP_031742561.1 uncharacterized protein At1g10890 isoform X1 [Cucumis sativus] >XP_031742562.1 uncharacterized protein At1g10890 isoform X1 [Cucumis sativus])

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-79
Identity = 224/394 (56.85%), Postives = 251/394 (63.71%), Query Frame = 0

Query: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLDCDFA 60
           MPRDLSRSRSP YRRR SPSP+GHRYTRRSRRDRSRSPYSSYSRL VLMGV SLLDCDF+
Sbjct: 1   MPRDLSRSRSPLYRRRLSPSPVGHRYTRRSRRDRSRSPYSSYSRLWVLMGVPSLLDCDFS 60

Query: 61  SSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLYIVDL 120
           SSCDVIKDGAHK                           F  + +S+             
Sbjct: 61  SSCDVIKDGAHKG-------------------------LFLTIRKSRS------------ 120

Query: 121 LVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLGCRHCFAHQNPLFFLLISIFVLIILL 180
                  +S +R    +   R+ +  +P        R                       
Sbjct: 121 -------ISPRRNRSRSRTPRHHRSRSPTSRSYKKQRR---------------------- 180

Query: 181 VVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQETEA 240
                    S    L    +  S G  E +     +  +++            RQQET+ 
Sbjct: 181 --------RSSSSSLHRRSSSSSLGSIEQKSTSEKLKKEEE---------RKRRQQETQG 240

Query: 241 KLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKEAALV 300
           KLLKEETTKRVE+AI KEVE+ LNSDD+KL+I KKLEEGR RLNEEV+AQLEKEKEAALV
Sbjct: 241 KLLKEETTKRVEDAIRKEVEERLNSDDVKLDINKKLEEGRTRLNEEVTAQLEKEKEAALV 300

Query: 301 EARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEAI 360
           EARR+EE+ARKEKEE+ERMVEE+RRRVEEAQRREALERQKREEERYRELEELQRQKEEAI
Sbjct: 301 EARRREEQARKEKEELERMVEESRRRVEEAQRREALERQKREEERYRELEELQRQKEEAI 311

Query: 361 KRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 395
           KRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK
Sbjct: 361 KRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 311

BLAST of Lsi03G008740 vs. NCBI nr
Match: XP_023529906.1 (uncharacterized protein At1g10890-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529907.1 uncharacterized protein At1g10890-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.8 bits (774), Expect = 4.5e-78
Identity = 222/395 (56.20%), Postives = 249/395 (63.04%), Query Frame = 0

Query: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLDCDFA 60
           MPRDLSRSRSPSYRRRHSPSPLGHRY+RRSRRDRSRSPYSSYSRL VLMGVLSLLDCDFA
Sbjct: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYSRRSRRDRSRSPYSSYSRLWVLMGVLSLLDCDFA 60

Query: 61  SSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLYIVDL 120
           SSCDVIKDGAHK                           F  + +S+             
Sbjct: 61  SSCDVIKDGAHKG-------------------------LFLTIRKSR------------- 120

Query: 121 LVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLG-CRHCFAHQNPLFFLLISIFVLIIL 180
              ++ P             R+    TP  H         +  Q                
Sbjct: 121 ---SISP------------RRHRSSRTPRRHRSRSPTSRSYRKQR--------------- 180

Query: 181 LVVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQETE 240
                 S   S  P   G     S G  E +        +++            RQ+ TE
Sbjct: 181 ----RRSSSSSLHPRSSG----SSPGSIEQKSTSEKFRKEEE--------ERKRRQKVTE 240

Query: 241 AKLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKEAAL 300
           AK L++ETTKR+EEAI K+VE SLN DD+K+EI  K+EEGRRRLNEEV+AQLEKEKEAAL
Sbjct: 241 AKFLEKETTKRLEEAIRKKVEASLNDDDVKVEINSKMEEGRRRLNEEVAAQLEKEKEAAL 300

Query: 301 VEARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEA 360
           +EA+RKEEEARKEKEEVERMVEE RRRVEEAQR EALERQ+REEERYRELEELQR+KEEA
Sbjct: 301 IEAQRKEEEARKEKEEVERMVEERRRRVEEAQRSEALERQQREEERYRELEELQRKKEEA 311

Query: 361 IKRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 395
           IKRKKQEE+EQR+NQMKLLGKNKSRPKLSFAIGSK
Sbjct: 361 IKRKKQEEDEQRLNQMKLLGKNKSRPKLSFAIGSK 311

BLAST of Lsi03G008740 vs. NCBI nr
Match: XP_022989689.1 (uncharacterized protein At1g10890-like isoform X1 [Cucurbita maxima] >XP_022989690.1 uncharacterized protein At1g10890-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 298.1 bits (762), Expect = 1.1e-76
Identity = 222/395 (56.20%), Postives = 251/395 (63.54%), Query Frame = 0

Query: 1   MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLDCDFA 60
           MPRDLSRSRSPSYRRR SPSPLGHRY+RRSRRDRSRSPYSSYSRL VLMGVLSLLDCDFA
Sbjct: 1   MPRDLSRSRSPSYRRRLSPSPLGHRYSRRSRRDRSRSPYSSYSRLWVLMGVLSLLDCDFA 60

Query: 61  SSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLYIVDL 120
           SSCDVIKDGAHK                           F  + +S+             
Sbjct: 61  SSCDVIKDGAHKG-------------------------LFLTIRKSRS------------ 120

Query: 121 LVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLG-CRHCFAHQNPLFFLLISIFVLIIL 180
                  +S +R     H++R+    TP  H         +  Q                
Sbjct: 121 -------ISPRR-----HRSRS---RTPRRHRSRSPTSRSYRKQR--------------- 180

Query: 181 LVVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQETE 240
                 S   S  P   G     S G  E +        +++            RQ+ TE
Sbjct: 181 ----RRSSSSSLHPRSSG----SSPGSIEQKSTSEKFRKEEE--------ERKRRQKVTE 240

Query: 241 AKLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKEAAL 300
           AK L++ET KR+EEAI K+VE SLN +D+KL I +K+EEGRRRLNEEV+AQLEKEKEAAL
Sbjct: 241 AKFLEKETAKRLEEAIRKKVEASLNDEDVKLGINRKMEEGRRRLNEEVTAQLEKEKEAAL 300

Query: 301 VEARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQRQKEEA 360
           +EA+RKEEEARKEKEEVERMVEE RRRVEEAQR EALERQ+REEERYRELEELQR+KEEA
Sbjct: 301 IEAQRKEEEARKEKEEVERMVEERRRRVEEAQRSEALERQQREEERYRELEELQRKKEEA 312

Query: 361 IKRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 395
           IKRKKQEEEEQR+NQMKLLGKNKSRPKLSFAIGSK
Sbjct: 361 IKRKKQEEEEQRLNQMKLLGKNKSRPKLSFAIGSK 312

BLAST of Lsi03G008740 vs. TAIR 10
Match: AT1G10890.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: petal, flower, leaf; EXPRESSED DURING: LP.04 four leaves visible, 4 anthesis, petal differentiation and expansion stage; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G13340.1); Has 11769 Blast hits to 8435 proteins in 698 species: Archae - 22; Bacteria - 971; Metazoa - 5937; Fungi - 1065; Plants - 592; Viruses - 101; Other Eukaryotes - 3081 (source: NCBI BLink). )

HSP 1 Score: 174.5 bits (441), Expect = 1.7e-43
Identity = 166/406 (40.89%), Postives = 211/406 (51.97%), Query Frame = 0

Query: 1   MPRDLSRSR----SPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSSYSRLRVLMGVLSLLD 60
           MPRDLSRSR    SPS RR+HS SP+  R++RRSRRDRS SPYSS+S  R          
Sbjct: 1   MPRDLSRSRSPSPSPSRRRKHSRSPVRQRHSRRSRRDRSPSPYSSHSYSR---------- 60

Query: 61  CDFASSCDVIKDGAHKEEKAAQFLQDVIEVVHEHQDAIEVVLQFQGVTRSKDGGVPHHLY 120
                    I    H+                            + VT  +    P    
Sbjct: 61  ----RKSRSISPRRHRS---------------------------RSVTPKRRSPTP---- 120

Query: 121 IVDLLVLALDPLSKKRVLLLTHKTRNGQFHTPAGHHVLGCRHCFAHQNPLFFLLISIFVL 180
                         KR     +K +  +  TP+           A ++P           
Sbjct: 121 --------------KR-----YKRQKSRSSTPSP----------AKRSPA---------- 180

Query: 181 IILLVVVNNSLLLSCMPILKGYLTFFSTGFSEGRYCYRLVIFDDQFGASLICCSPVLRQQ 240
                                  T  S     G    R    +++            RQ+
Sbjct: 181 ----------------------ATLESAKNRNGEKLKR----EEE--------ERKRRQR 240

Query: 241 ETEAKLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKEKE 300
           E E KL++EET KRVEEAI K+VE+SL S+ +K+EI+  LEEGR+RLNEEV+AQLE+EKE
Sbjct: 241 EAELKLIEEETVKRVEEAIRKKVEESLQSEKIKMEILTLLEEGRKRLNEEVAAQLEEEKE 288

Query: 301 AALVEARRKE--------EEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRE 360
           A+L+EA+ KE        E  ++EKEE ER+ EEN +RVEEAQR+EA+ERQ++EEERYRE
Sbjct: 301 ASLIEAKEKEGVMRCLSQEREQQEKEERERIAEENLKRVEEAQRKEAMERQRKEEERYRE 288

Query: 361 LEELQRQKEEAIKRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIGSK 395
           LEELQRQKEEA++RKK EEEE+R+ QMKLLGKNKSRPKLSFA+ SK
Sbjct: 361 LEELQRQKEEAMRRKKAEEEEERLKQMKLLGKNKSRPKLSFALSSK 288

BLAST of Lsi03G008740 vs. TAIR 10
Match: AT5G13340.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G10890.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 131.7 bits (330), Expect = 1.3e-30
Identity = 91/158 (57.59%), Postives = 123/158 (77.85%), Query Frame = 0

Query: 235 QQETEAKLLKEETTKRVEEAICKEVEQSLNSDDLKLEIVKKLEEGRRRLNEEVSAQLEKE 294
           Q E E K L+EET +R+EEA+ K VE+ + ++++K EI ++ +E   ++  +V  QL+KE
Sbjct: 84  QHEAELKRLEEETAQRIEEAVRKNVEERMKTEEVKEEIERRTKEAYEKMFLDVEIQLKKE 143

Query: 295 KEAALVEARRKEEEARKEKEEVERMVEENRRRVEEAQRREALERQKREEERYRELEELQR 354
           KEAAL EARRKEE+AR+E+EE+++M+EEN RRVEE+QRREA+E Q++EEERYRELE LQR
Sbjct: 144 KEAALNEARRKEEQARREREELDKMLEENSRRVEESQRREAMELQRKEEERYRELELLQR 203

Query: 355 QKEEAIKRKKQEEEEQRVNQMKLLGKNKSRPKLSFAIG 393
           QKEEA +RKK EEEE+  N  KL   N+SR KL F +G
Sbjct: 204 QKEEAARRKKLEEEEEIRNSSKLSNGNRSRSKLHFGMG 241


HSP 2 Score: 53.1 bits (126), Expect = 5.7e-07
Identity = 29/41 (70.73%), Postives = 36/41 (87.80%), Query Frame = 0

Query: 1  MPRDLSRSRSPSYRRRHSPSPLGHRYTRRSRRDRSRSPYSS 42
          M R+ SRSRSPS+RRR+S SP+ HR +RR+RRDRSRSPY+S
Sbjct: 1  MARNDSRSRSPSHRRRYSRSPVTHRSSRRTRRDRSRSPYTS 41

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0CB261.5e-4441.71Uncharacterized protein At1g10890 OS=Arabidopsis thaliana OX=3702 GN=At1g10890 P... [more]
Q2TA421.1e-1539.75Arginine and glutamate-rich protein 1 OS=Bos taurus OX=9913 GN=ARGLU1 PE=2 SV=1[more]
Q9NWB61.1e-1539.75Arginine and glutamate-rich protein 1 OS=Homo sapiens OX=9606 GN=ARGLU1 PE=1 SV=... [more]
Q3UL361.1e-1539.75Arginine and glutamate-rich protein 1 OS=Mus musculus OX=10090 GN=Arglu1 PE=1 SV... [more]
Q5BJT01.1e-1539.75Arginine and glutamate-rich protein 1 OS=Rattus norvegicus OX=10116 GN=Arglu1 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1EIS13.9e-8056.35uncharacterized protein At1g10890-like isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1JQ205.3e-7756.20uncharacterized protein At1g10890-like isoform X1 OS=Cucurbita maxima OX=3661 GN... [more]
A0A0A0KEG01.4e-6150.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G411270 PE=4 SV=1[more]
A0A5A7UT491.6e-6051.02Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1EIF01.0e-5949.87uncharacterized protein At1g10890-like isoform X2 OS=Cucurbita moschata OX=3662 ... [more]
Match NameE-valueIdentityDescription
XP_038888588.14.2e-8459.14uncharacterized protein At1g10890 isoform X3 [Benincasa hispida][more]
XP_022927714.18.1e-8056.35uncharacterized protein At1g10890-like isoform X1 [Cucurbita moschata] >XP_02292... [more]
XP_031742559.11.1e-7956.85uncharacterized protein At1g10890 isoform X1 [Cucumis sativus] >XP_031742560.1 u... [more]
XP_023529906.14.5e-7856.20uncharacterized protein At1g10890-like isoform X1 [Cucurbita pepo subsp. pepo] >... [more]
XP_022989689.11.1e-7656.20uncharacterized protein At1g10890-like isoform X1 [Cucurbita maxima] >XP_0229896... [more]
Match NameE-valueIdentityDescription
AT1G10890.11.7e-4340.89unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT5G13340.11.3e-3057.59unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 273..370
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 308..375
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 12..32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 308..394
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..38
IPR033371Arginine and glutamate-rich protein 1PFAMPF15346ARGLUcoord: 236..390
e-value: 4.3E-36
score: 124.0
IPR033371Arginine and glutamate-rich protein 1PANTHERPTHR31711ARGININE AND GLUTAMATE-RICH PROTEIN 1coord: 1..45
coord: 234..394

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G008740.1Lsi03G008740.1mRNA