Lsi03G008650 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G008650
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionVery-long-chain 3-oxoacyl-CoA synthase
Locationchr03: 14588496 .. 14589632 (-)
RNA-Seq ExpressionLsi03G008650
SyntenyLsi03G008650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTTCATTTTTCTCCATTGAGTTACAGTTCAAGCTCCACCGCCTTTGCTCACCCCACATTTTTTGAAATTTTTGGACAAATTTCTCAGAGCTTTGGTTCCCGACTCAAATCTCAGGTTCCGAACCCTAATTTTTATTCGTTTACTTCATTTAATTGTTTGCTAAACTAAATTGTTATCTGAATTAGTTTATTAGGGATCTTTGCTTTCCCTTGTCCTTGAAATAAACATAGAACGAGTCTCTGTCGATGCATTATCTCTTCGAAATTCCTTAGTCTATTTCCTCTCATTTGGGTGTCTCGTAAAGTACAGAATGAATCAAATTCTCTCAGTTCTTCGATATTGGCTTGTGAATCATCCGATTATCCTCAATTTCGAATGGATTCAAGGTCAAACCTTCGCCTCCACGCCCCTTTTCCTCATCCTCACTGTTTTCTCTTACCTATTCCTCACCTTCCTCCTCTCCCACATCCCCATTCCTTCCATTTCCCCCCACTTTCTCAAGCCAATCTCAGCCCTTCACAACTTTGTCCTTCTCATCCTCTCCTTCATCATGGCCCTTGGATGCACTCTCTCTTCCATTTACCATGTGCCTCATCTCTACTGGATTGTCTGCTTTCCACCTCGCACCCCACCAGTTGGACCTCTCTTCTTCTGGGCTTATGTCTTCTACCTCTCCAAGATTCTTGAATTCATCGACACCCTCTTGATCATCCTCACCGGGTCGTTTCAACGCCTCACATTCCTCCATGTTTACCACCATTCGACGGTTTTGATCATGTGTTATCTCTGGCTTCATACTTCCCAATCACTGTTCCCCATAGCACTCGTGACCAATGCGACGGTGCACGTCATAATGTATGGATACTACTTCCTATGCACCTTTGGGATTAGGCCAAAGTGGAAGAGGTTGGTCACTGACTGCCAAATCTTACAGTTTGTGTTTAGTTTTGTGGTCTCGGGTCAAATGTTGTATGAGCATTTTGGTGGATCTGTTGCTGGTTGCTCTGGCTTTTGGGGTTGGTGCTTTAATGCTGTTTTCAATGGCTCTCTTTTAGCTCTTTTCATCAACTTCCATTTGAAAAGTTATGCTGCCAATAGGAAGAAGAAGAGAATAGAATCTAAAACCCAATGA

mRNA sequence

ATGGCTTCAAGCTCCACCGCCTTTGCTCACCCCACATTTTTTGAAATTTTTGGACAAATTTCTCAGAGCTTTGGTTCCCGACTCAAATCTCAGGGATCTTTGCTTTCCCTTGTCCTTGAAATAAACATAGAACGAGTCTCTGTCGATGCATTATCTCTTCGAAATTCCTTAGTCTATTTCCTCTCATTTGGGTGTCTCGTAAAGTACAGAATGAATCAAATTCTCTCAGTTCTTCGATATTGGCTTGTGAATCATCCGATTATCCTCAATTTCGAATGGATTCAAGGTCAAACCTTCGCCTCCACGCCCCTTTTCCTCATCCTCACTGTTTTCTCTTACCTATTCCTCACCTTCCTCCTCTCCCACATCCCCATTCCTTCCATTTCCCCCCACTTTCTCAAGCCAATCTCAGCCCTTCACAACTTTGTCCTTCTCATCCTCTCCTTCATCATGGCCCTTGGATGCACTCTCTCTTCCATTTACCATGTGCCTCATCTCTACTGGATTGTCTGCTTTCCACCTCGCACCCCACCAGTTGGACCTCTCTTCTTCTGGGCTTATGTCTTCTACCTCTCCAAGATTCTTGAATTCATCGACACCCTCTTGATCATCCTCACCGGGTCGTTTCAACGCCTCACATTCCTCCATGTTTACCACCATTCGACGGTTTTGATCATGTGTTATCTCTGGCTTCATACTTCCCAATCACTGTTCCCCATAGCACTCGTGACCAATGCGACGGTGCACGTCATAATGTATGGATACTACTTCCTATGCACCTTTGGGATTAGGCCAAAGTGGAAGAGGTTGGTCACTGACTGCCAAATCTTACAGTTTGTGTTTAGTTTTGTGGTCTCGGGTCAAATGTTGTATGAGCATTTTGGTGGATCTGTTGCTGGTTGCTCTGGCTTTTGGGGTTGGTGCTTTAATGCTGTTTTCAATGGCTCTCTTTTAGCTCTTTTCATCAACTTCCATTTGAAAAGTTATGCTGCCAATAGGAAGAAGAAGAGAATAGAATCTAAAACCCAATGA

Coding sequence (CDS)

ATGGCTTCAAGCTCCACCGCCTTTGCTCACCCCACATTTTTTGAAATTTTTGGACAAATTTCTCAGAGCTTTGGTTCCCGACTCAAATCTCAGGGATCTTTGCTTTCCCTTGTCCTTGAAATAAACATAGAACGAGTCTCTGTCGATGCATTATCTCTTCGAAATTCCTTAGTCTATTTCCTCTCATTTGGGTGTCTCGTAAAGTACAGAATGAATCAAATTCTCTCAGTTCTTCGATATTGGCTTGTGAATCATCCGATTATCCTCAATTTCGAATGGATTCAAGGTCAAACCTTCGCCTCCACGCCCCTTTTCCTCATCCTCACTGTTTTCTCTTACCTATTCCTCACCTTCCTCCTCTCCCACATCCCCATTCCTTCCATTTCCCCCCACTTTCTCAAGCCAATCTCAGCCCTTCACAACTTTGTCCTTCTCATCCTCTCCTTCATCATGGCCCTTGGATGCACTCTCTCTTCCATTTACCATGTGCCTCATCTCTACTGGATTGTCTGCTTTCCACCTCGCACCCCACCAGTTGGACCTCTCTTCTTCTGGGCTTATGTCTTCTACCTCTCCAAGATTCTTGAATTCATCGACACCCTCTTGATCATCCTCACCGGGTCGTTTCAACGCCTCACATTCCTCCATGTTTACCACCATTCGACGGTTTTGATCATGTGTTATCTCTGGCTTCATACTTCCCAATCACTGTTCCCCATAGCACTCGTGACCAATGCGACGGTGCACGTCATAATGTATGGATACTACTTCCTATGCACCTTTGGGATTAGGCCAAAGTGGAAGAGGTTGGTCACTGACTGCCAAATCTTACAGTTTGTGTTTAGTTTTGTGGTCTCGGGTCAAATGTTGTATGAGCATTTTGGTGGATCTGTTGCTGGTTGCTCTGGCTTTTGGGGTTGGTGCTTTAATGCTGTTTTCAATGGCTCTCTTTTAGCTCTTTTCATCAACTTCCATTTGAAAAGTTATGCTGCCAATAGGAAGAAGAAGAGAATAGAATCTAAAACCCAATGA

Protein sequence

MASSSTAFAHPTFFEIFGQISQSFGSRLKSQGSLLSLVLEINIERVSVDALSLRNSLVYFLSFGCLVKYRMNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISPHFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLYWIVCFPPRTPPVGPLFFWAYVFYLSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHVIMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFWGWCFNAVFNGSLLALFINFHLKSYAANRKKKRIESKTQ
Homology
BLAST of Lsi03G008650 vs. ExPASy Swiss-Prot
Match: Q9SYY4 (Elongation of fatty acids protein 3-like OS=Arabidopsis thaliana OX=3702 GN=HOS3 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.4e-24
Identity = 83/280 (29.64%), Postives = 145/280 (51.79%), Query Frame = 0

Query: 74  ILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISPHF- 133
           +++ + Y+L  HP I+ F W   Q++ ST  FL  ++  Y+ ++  L HI + ++     
Sbjct: 5   LINSITYFLSEHPYIVGFRWSNSQSWGSTWSFLFTSISLYIAVSSSL-HILLSAVRRSNR 64

Query: 134 ---LKPISALHNFVLLILSFIMALGCTLSSIYHV-------------PHLYWIVCFPPRT 193
              L  I  +H+ ++ ILS  +  G  LS+   +               L W++CFP  T
Sbjct: 65  SVPLGHIPEIHSLLMSILSATIFAGILLSAAAEIRDTRWLWRRSKTATPLQWLLCFPLGT 124

Query: 194 PPVGPLFFWAYVFYLSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQS 253
            P G +FFW+YVFYL++ L    T+  +L    +RL    ++ +S +    +LWL  SQS
Sbjct: 125 RPSGRVFFWSYVFYLTRFLHMFRTIFAVLRS--RRLAVSQLFCNSVMAFTSFLWLEFSQS 184

Query: 254 LFPIALVTNATVHVIMYGYYFLCTFGI-RPKWKRLVTDCQILQFVFSFVVSGQMLYEH-F 313
              +A+++   V+ ++YGY F   FG+    +   V +CQ++    + V    +L  H F
Sbjct: 185 YQILAILSTTLVYSVVYGYRFWTGFGLPGSAFPSFVVNCQLVLVGCNLVSHAGVLTMHLF 244

Query: 314 GGSVAGCSGFWGWCFNAVFNGSLLALFINFHLKSYAANRK 335
            G   GC+G   W  N+V NG++L LF+NF+++ ++  R+
Sbjct: 245 KG---GCNGIGAWGLNSVLNGAILLLFLNFYVRMHSPMRR 278

BLAST of Lsi03G008650 vs. ExPASy Swiss-Prot
Match: Q54CJ4 (Elongation of fatty acids protein A OS=Dictyostelium discoideum OX=44689 GN=eloA PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 3.5e-21
Identity = 94/260 (36.15%), Postives = 129/260 (49.62%), Query Frame = 0

Query: 91  FEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISPHFLKPISALHNFVLLILSFI 150
           F W    T  S  LF I+  F YL L + L  I + +     L   +  HN  L +LS +
Sbjct: 23  FRWKNEVTPFSNILFPIVCSFGYLALIYGL-QIFMKNKKEIKLHGFAMFHNLFLCLLSLL 82

Query: 151 MALGCTL-SSIYHVPH-LYWIVCFPPRTPPVGPLFFWAYVFYLSKILEFIDTLLIILTGS 210
           M LG  +  + Y  PH LY I+C P  +   G + F  Y+FYLSK+ EFIDT++ +L   
Sbjct: 83  MFLGIVIPMAKYSFPHGLYNIICKPIDS---GLVQFSYYIFYLSKVYEFIDTIIQVLRK- 142

Query: 211 FQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHVIMYGYYFLCTFGIRPKWK 270
            + L FLHV+HH   L + +  L        + +  N  VH++MY YYF    GI P WK
Sbjct: 143 -KSLLFLHVWHHFITLWLVWANLKYDTGCQWVDISANCFVHIVMYFYYFQTERGINPWWK 202

Query: 271 RLVTDCQILQFVFSFVVSGQMLYEHFGGSVAG-----CSGFWGW-CFNAVFNGSLLALFI 330
           + +T CQI+QF+   V     L  HF  +        CSG W    F+     S L LFI
Sbjct: 203 KHITTCQIIQFI---VDMSSHLAWHFYDTQGNHNSNYCSGTWATSAFSDFVILSFLGLFI 262

Query: 331 NFHLKSYAANRKKKRIESKT 343
            F +K+Y   +KK  I+ KT
Sbjct: 263 QFFVKAY---KKKSSIKKKT 270

BLAST of Lsi03G008650 vs. ExPASy Swiss-Prot
Match: Q86JM5 (Putative elongation of fatty acids protein DDB_G0272012 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0272012 PE=3 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 8.0e-18
Identity = 76/245 (31.02%), Postives = 125/245 (51.02%), Query Frame = 0

Query: 89  LNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISPHFLKPISALHNFVLLILS 148
           +NF++  G T  S    L   +  YL + F +  + + +  P  LK +S LHN +L I S
Sbjct: 37  VNFKFKFGTTPFSQFQILPSVISLYLVIIFSIKFL-MRNRKPFSLKYVSILHNAILCIWS 96

Query: 149 FIMALGCTLSSIYHV--PHLYWIVCFPPRTPPVGPLFFWAYVFYLSKILEFIDTLLIILT 208
            +M +G     I  +      + VC        GP ++W+Y+FY+SK  E +DT++I+L 
Sbjct: 97  LVMCVGILYEVIKRITAEGPLFTVCETVSGFDKGPAYYWSYIFYISKFYELLDTVIIVL- 156

Query: 209 GSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHVIMYGYYFLCTFGIRPK 268
              + L FLHVYHH  V+ +C+ ++++  +L    +  N  VHV MY +YF    G    
Sbjct: 157 -KKKPLIFLHVYHHCIVVWLCWYFMYSGWNLQLWVVFLNTFVHVFMYYFYFQTGRGKTVW 216

Query: 269 WKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFWGWCFNA-VFNGSLLALFINFH 328
           WK+ +T  QI+QF+    ++G +       + + C   +    +A + N S L LF  F 
Sbjct: 217 WKKYITMIQIIQFI-CLGIAGLLHSAAINLNSSPCFTHYPAFISAYLINFSFLFLFSQFF 276

Query: 329 LKSYA 331
           +KSY+
Sbjct: 277 VKSYS 277

BLAST of Lsi03G008650 vs. ExPASy Swiss-Prot
Match: P39540 (Elongation of fatty acids protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ELO1 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.0e-17
Identity = 88/266 (33.08%), Postives = 136/266 (51.13%), Query Frame = 0

Query: 90  NFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISPHFLKPISALHNFVLLILSF 149
           +FE+  G+   S P  ++L +  Y  + F    + + S  P  L+ IS +HN +L  +SF
Sbjct: 49  DFEFTVGKQPLSEPRPVLLFIAMYYVVIFGGRSL-VKSCKPLKLRFISQVHNLMLTSVSF 108

Query: 150 IMALGCT--LSSIYHVPHLYWIVC-FPPRTPPVGPLFFWAYVFYLSKILEFIDTLLIILT 209
           +  +     +  I +   LY+ VC     T P+  L+   Y+ Y++K +EF DT+L++L 
Sbjct: 109 LWLILMVEQMLPIVYRHGLYFAVCNVESWTQPMETLY---YLNYMTKFVEFADTVLMVL- 168

Query: 210 GSFQRLTFLHVYHHSTVLIMCYLWL--HTSQSLFPIALVTNATVHVIMYGYYFLCTFGIR 269
              ++LTFLH YHH    ++CY  L  +T+ +  P+ L  N  VHV+MY YYFL   GIR
Sbjct: 169 -KHRKLTFLHTYHHGATALLCYNQLVGYTAVTWVPVTL--NLAVHVLMYWYYFLSASGIR 228

Query: 270 PKWKRLVTDCQILQFVFSFVVSGQMLYEHF------GGSVAGCSGFWGWCFNAVFNG--- 329
             WK  VT  QI+QF+   +V   +LY+              C    G    A+  G   
Sbjct: 229 VWWKAWVTRLQIVQFMLDLIVVYYVLYQKIVAAYFKNACTPQCEDCLG-SMTAIAAGAAI 288

Query: 330 --SLLALFINFHLKSY---AANRKKK 337
             S L LFI+F+++ Y   +A+ KKK
Sbjct: 289 LTSYLFLFISFYIEVYKRGSASGKKK 305

BLAST of Lsi03G008650 vs. ExPASy Swiss-Prot
Match: P40319 (Elongation of fatty acids protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ELO3 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 4.0e-17
Identity = 79/258 (30.62%), Postives = 124/258 (48.06%), Query Frame = 0

Query: 91  FEWIQGQTFASTPLFLILTVFSYLFLTF-LLSHIPIPSISPHFLKPISALHNFVLLILS- 150
           FE+I  +TF +     +  +  Y  + F   + +   + SP   K +  +HN  L  +S 
Sbjct: 61  FEFIHNKTFLANGYHAVSIIIVYYIIIFGGQAILRALNASPLKFKLLFEIHNLFLTSISL 120

Query: 151 --FIMALGCTLSSIYHVPHLYWIVCFPPRTPPVGPLFFWAYVFYLSKILEFIDTLLIILT 210
             +++ L   +  +YH   L+W +C      P   L    Y+ YL+K +E IDT+ ++L 
Sbjct: 121 VLWLLMLEQLVPMVYH-NGLFWSICSKEAFAP--KLVTLYYLNYLTKFVELIDTVFLVLR 180

Query: 211 GSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHVIMYGYYFLCTFGIRPK 270
              ++L FLH YHH    ++CY  L    S+  + ++ N  VHVIMY YYFL + GIR  
Sbjct: 181 R--KKLLFLHTYHHGATALLCYTQLIGRTSVEWVVILLNLGVHVIMYWYYFLSSCGIRVW 240

Query: 271 WKRLVTDCQILQFVFSFVVSGQMLYEHFGGS-VAGCSGFWGWCFNA--------VFNGSL 330
           WK+ VT  QI+QF+   V      Y  +    + G     G C+          +   S 
Sbjct: 241 WKQWVTRFQIIQFLIDLVFVYFATYTFYAHKYLDGILPNKGTCYGTQAAAAYGYLILTSY 300

Query: 331 LALFINFHLKSYAANRKK 336
           L LFI+F+++SY    KK
Sbjct: 301 LLLFISFYIQSYKKGGKK 313

BLAST of Lsi03G008650 vs. ExPASy TrEMBL
Match: A0A0A0KJH8 (Very-long-chain 3-oxoacyl-CoA synthase OS=Cucumis sativus OX=3659 GN=Csa_6G411300 PE=4 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 5.2e-145
Identity = 262/273 (95.97%), Postives = 267/273 (97.80%), Query Frame = 0

Query: 71  MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISP 130
           MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLI+TVF+YLFLTFLLSHIPIPSIS 
Sbjct: 1   MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLIVTVFTYLFLTFLLSHIPIPSISS 60

Query: 131 HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLYWIVCFPPRTPPVGPLFFWAYVFY 190
           HFLK ISALHNFVLLILSFIMALGCTLSSIYHVPHL+WI+CFPPRTPPVGPLFFWAYVFY
Sbjct: 61  HFLKSISALHNFVLLILSFIMALGCTLSSIYHVPHLHWIICFPPRTPPVGPLFFWAYVFY 120

Query: 191 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 250
           LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV
Sbjct: 121 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 180

Query: 251 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFWGWCFN 310
           IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLY+HFGGS  GCSGF GWCFN
Sbjct: 181 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYDHFGGSAGGCSGFLGWCFN 240

Query: 311 AVFNGSLLALFINFHLKSYAANRKKKRIESKTQ 344
           AVFNGSLLALFINFHLKSYAANRKKK IESKTQ
Sbjct: 241 AVFNGSLLALFINFHLKSYAANRKKK-IESKTQ 272

BLAST of Lsi03G008650 vs. ExPASy TrEMBL
Match: A0A5A7UQE3 (Very-long-chain 3-oxoacyl-CoA synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold496G00180 PE=4 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 8.9e-145
Identity = 261/273 (95.60%), Postives = 265/273 (97.07%), Query Frame = 0

Query: 71  MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISP 130
           MNQILSVLRYWLVNHP IL FEWIQGQTFASTPLFLILTVF+YLFLTFLLSHIPIPS SP
Sbjct: 1   MNQILSVLRYWLVNHPSILKFEWIQGQTFASTPLFLILTVFTYLFLTFLLSHIPIPSFSP 60

Query: 131 HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLYWIVCFPPRTPPVGPLFFWAYVFY 190
           HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHL+WI+CFPP TPPVGPLFFWAYVFY
Sbjct: 61  HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLHWIICFPPHTPPVGPLFFWAYVFY 120

Query: 191 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 250
           LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV
Sbjct: 121 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 180

Query: 251 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFWGWCFN 310
           IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLY+HFGGS  GCSGF GWCFN
Sbjct: 181 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYDHFGGSAGGCSGFLGWCFN 240

Query: 311 AVFNGSLLALFINFHLKSYAANRKKKRIESKTQ 344
           AVFNGSLLALFINFHLKSYAANR KKRIESKTQ
Sbjct: 241 AVFNGSLLALFINFHLKSYAANR-KKRIESKTQ 272

BLAST of Lsi03G008650 vs. ExPASy TrEMBL
Match: A0A1S4DXJ2 (Very-long-chain 3-oxoacyl-CoA synthase OS=Cucumis melo OX=3656 GN=LOC103491307 PE=4 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 8.9e-145
Identity = 261/273 (95.60%), Postives = 265/273 (97.07%), Query Frame = 0

Query: 71  MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISP 130
           MNQILSVLRYWLVNHP IL FEWIQGQTFASTPLFLILTVF+YLFLTFLLSHIPIPS SP
Sbjct: 1   MNQILSVLRYWLVNHPSILKFEWIQGQTFASTPLFLILTVFTYLFLTFLLSHIPIPSFSP 60

Query: 131 HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLYWIVCFPPRTPPVGPLFFWAYVFY 190
           HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHL+WI+CFPP TPPVGPLFFWAYVFY
Sbjct: 61  HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLHWIICFPPHTPPVGPLFFWAYVFY 120

Query: 191 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 250
           LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV
Sbjct: 121 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 180

Query: 251 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFWGWCFN 310
           IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLY+HFGGS  GCSGF GWCFN
Sbjct: 181 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYDHFGGSAGGCSGFLGWCFN 240

Query: 311 AVFNGSLLALFINFHLKSYAANRKKKRIESKTQ 344
           AVFNGSLLALFINFHLKSYAANR KKRIESKTQ
Sbjct: 241 AVFNGSLLALFINFHLKSYAANR-KKRIESKTQ 272

BLAST of Lsi03G008650 vs. ExPASy TrEMBL
Match: A0A6J1EPN2 (Very-long-chain 3-oxoacyl-CoA synthase OS=Cucurbita moschata OX=3662 GN=LOC111434494 PE=4 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 2.1e-138
Identity = 247/272 (90.81%), Postives = 259/272 (95.22%), Query Frame = 0

Query: 71  MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISP 130
           MNQIL  LRYWLVNHPIIL+FEWIQGQTF STPLFLILTVFSYLFLT LLSHIP+PSISP
Sbjct: 1   MNQILPDLRYWLVNHPIILDFEWIQGQTFGSTPLFLILTVFSYLFLTLLLSHIPLPSISP 60

Query: 131 HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLYWIVCFPPRTPPVGPLFFWAYVFY 190
           HFLKPISALHNFVLL LSFIMALGC LSSIYHVPHL+W++CFPPRTPPVGPLFFWAYVFY
Sbjct: 61  HFLKPISALHNFVLLSLSFIMALGCALSSIYHVPHLHWLICFPPRTPPVGPLFFWAYVFY 120

Query: 191 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 250
           LSKILEF+DTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIAL+TNA VHV
Sbjct: 121 LSKILEFVDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALLTNAVVHV 180

Query: 251 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFWGWCFN 310
           IMYGYYFLCTFGIRP+WKRLVTDCQILQF+FSFV+SGQMLYEHFGGS  GCSG  GWCFN
Sbjct: 181 IMYGYYFLCTFGIRPRWKRLVTDCQILQFIFSFVISGQMLYEHFGGSGGGCSGIRGWCFN 240

Query: 311 AVFNGSLLALFINFHLKSYAANRKKKRIESKT 343
           AVFN SLLALFINFHLKSYAANR KKR+E+KT
Sbjct: 241 AVFNASLLALFINFHLKSYAANR-KKRMETKT 271

BLAST of Lsi03G008650 vs. ExPASy TrEMBL
Match: A0A6J1JN46 (Very-long-chain 3-oxoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC111486707 PE=4 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 1.0e-137
Identity = 247/272 (90.81%), Postives = 258/272 (94.85%), Query Frame = 0

Query: 71  MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISP 130
           MNQIL  LRYWLVNHPIIL+FEWIQGQTF STPLFLILTVFSYLFLT LLSHIP+PS+S 
Sbjct: 1   MNQILPDLRYWLVNHPIILDFEWIQGQTFGSTPLFLILTVFSYLFLTLLLSHIPLPSVSL 60

Query: 131 HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLYWIVCFPPRTPPVGPLFFWAYVFY 190
           HFLKPISALHNFVLL LSFIMALGCTLSSIYHVPHL+W++CFPPRTPPVGPLFFWAYVFY
Sbjct: 61  HFLKPISALHNFVLLSLSFIMALGCTLSSIYHVPHLHWLICFPPRTPPVGPLFFWAYVFY 120

Query: 191 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 250
           LSKILEFIDTL IILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIAL+TNA VHV
Sbjct: 121 LSKILEFIDTLFIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALLTNAAVHV 180

Query: 251 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFWGWCFN 310
           +MYGYYFLCTFGIRP WKRLVTDCQILQF+FSFVVSGQMLYEHFGGS  GCSGF GWCFN
Sbjct: 181 LMYGYYFLCTFGIRPSWKRLVTDCQILQFIFSFVVSGQMLYEHFGGSGGGCSGFRGWCFN 240

Query: 311 AVFNGSLLALFINFHLKSYAANRKKKRIESKT 343
           AVFN SLLALFINFHLKSYAANR KKR+E+KT
Sbjct: 241 AVFNASLLALFINFHLKSYAANR-KKRMETKT 271

BLAST of Lsi03G008650 vs. NCBI nr
Match: XP_038886924.1 (elongation of fatty acids protein 3-like [Benincasa hispida])

HSP 1 Score: 528.9 bits (1361), Expect = 3.3e-146
Identity = 264/273 (96.70%), Postives = 269/273 (98.53%), Query Frame = 0

Query: 71  MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISP 130
           MNQILSVLRYWLVNHPIILNFEWI+GQTFASTPLFLILTVFSYLFLTFLLS IPIPSISP
Sbjct: 1   MNQILSVLRYWLVNHPIILNFEWIEGQTFASTPLFLILTVFSYLFLTFLLSRIPIPSISP 60

Query: 131 HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLYWIVCFPPRTPPVGPLFFWAYVFY 190
           +FLK ISALHNFVLLILSFIMALGCTLSSIY +PHL+WI+CFPPRTPPVGPLFFWAYVFY
Sbjct: 61  NFLKTISALHNFVLLILSFIMALGCTLSSIYRLPHLHWIICFPPRTPPVGPLFFWAYVFY 120

Query: 191 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 250
           LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV
Sbjct: 121 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 180

Query: 251 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFWGWCFN 310
           IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGF GWCFN
Sbjct: 181 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFGGWCFN 240

Query: 311 AVFNGSLLALFINFHLKSYAANRKKKRIESKTQ 344
           AVFNGSLLALFINFHLKSYAANRKKKRIESKTQ
Sbjct: 241 AVFNGSLLALFINFHLKSYAANRKKKRIESKTQ 273

BLAST of Lsi03G008650 vs. NCBI nr
Match: XP_004140108.1 (elongation of fatty acids protein 3-like [Cucumis sativus] >KGN47926.1 hypothetical protein Csa_002855 [Cucumis sativus])

HSP 1 Score: 523.9 bits (1348), Expect = 1.1e-144
Identity = 262/273 (95.97%), Postives = 267/273 (97.80%), Query Frame = 0

Query: 71  MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISP 130
           MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLI+TVF+YLFLTFLLSHIPIPSIS 
Sbjct: 1   MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLIVTVFTYLFLTFLLSHIPIPSISS 60

Query: 131 HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLYWIVCFPPRTPPVGPLFFWAYVFY 190
           HFLK ISALHNFVLLILSFIMALGCTLSSIYHVPHL+WI+CFPPRTPPVGPLFFWAYVFY
Sbjct: 61  HFLKSISALHNFVLLILSFIMALGCTLSSIYHVPHLHWIICFPPRTPPVGPLFFWAYVFY 120

Query: 191 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 250
           LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV
Sbjct: 121 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 180

Query: 251 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFWGWCFN 310
           IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLY+HFGGS  GCSGF GWCFN
Sbjct: 181 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYDHFGGSAGGCSGFLGWCFN 240

Query: 311 AVFNGSLLALFINFHLKSYAANRKKKRIESKTQ 344
           AVFNGSLLALFINFHLKSYAANRKKK IESKTQ
Sbjct: 241 AVFNGSLLALFINFHLKSYAANRKKK-IESKTQ 272

BLAST of Lsi03G008650 vs. NCBI nr
Match: XP_016900704.1 (PREDICTED: elongation of fatty acids protein 3-like [Cucumis melo] >KAA0057330.1 elongation of fatty acids protein 3-like [Cucumis melo var. makuwa] >TYK13405.1 elongation of fatty acids protein 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 523.1 bits (1346), Expect = 1.8e-144
Identity = 261/273 (95.60%), Postives = 265/273 (97.07%), Query Frame = 0

Query: 71  MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISP 130
           MNQILSVLRYWLVNHP IL FEWIQGQTFASTPLFLILTVF+YLFLTFLLSHIPIPS SP
Sbjct: 1   MNQILSVLRYWLVNHPSILKFEWIQGQTFASTPLFLILTVFTYLFLTFLLSHIPIPSFSP 60

Query: 131 HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLYWIVCFPPRTPPVGPLFFWAYVFY 190
           HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHL+WI+CFPP TPPVGPLFFWAYVFY
Sbjct: 61  HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLHWIICFPPHTPPVGPLFFWAYVFY 120

Query: 191 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 250
           LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV
Sbjct: 121 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 180

Query: 251 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFWGWCFN 310
           IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLY+HFGGS  GCSGF GWCFN
Sbjct: 181 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYDHFGGSAGGCSGFLGWCFN 240

Query: 311 AVFNGSLLALFINFHLKSYAANRKKKRIESKTQ 344
           AVFNGSLLALFINFHLKSYAANR KKRIESKTQ
Sbjct: 241 AVFNGSLLALFINFHLKSYAANR-KKRIESKTQ 272

BLAST of Lsi03G008650 vs. NCBI nr
Match: XP_023529341.1 (elongation of fatty acids protein 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 502.3 bits (1292), Expect = 3.3e-138
Identity = 249/272 (91.54%), Postives = 258/272 (94.85%), Query Frame = 0

Query: 71  MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISP 130
           MNQIL  LRYWLVNHPIIL+FEWIQGQTF STPLFLILTVFSYLFLT LLSHIP+PSISP
Sbjct: 1   MNQILPDLRYWLVNHPIILDFEWIQGQTFGSTPLFLILTVFSYLFLTLLLSHIPLPSISP 60

Query: 131 HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLYWIVCFPPRTPPVGPLFFWAYVFY 190
           HFLKPISALHNFVLL LSFIMALGC LSSIYHVPHL+W++CFPPRTPPVGPLFFWAYVFY
Sbjct: 61  HFLKPISALHNFVLLTLSFIMALGCALSSIYHVPHLHWLICFPPRTPPVGPLFFWAYVFY 120

Query: 191 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 250
           LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIAL+TNA VHV
Sbjct: 121 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALLTNAVVHV 180

Query: 251 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFWGWCFN 310
           IMYGYYFLCT GIRP WKRLVTDCQILQF+FSFVVSGQMLYEHFGGS  GCSGF GWCFN
Sbjct: 181 IMYGYYFLCTLGIRPSWKRLVTDCQILQFIFSFVVSGQMLYEHFGGSGGGCSGFRGWCFN 240

Query: 311 AVFNGSLLALFINFHLKSYAANRKKKRIESKT 343
           AVFN SLLALFINFHLKSYAANR KKR+E+KT
Sbjct: 241 AVFNASLLALFINFHLKSYAANR-KKRMETKT 271

BLAST of Lsi03G008650 vs. NCBI nr
Match: XP_022927675.1 (elongation of fatty acids protein 3-like [Cucurbita moschata])

HSP 1 Score: 501.9 bits (1291), Expect = 4.4e-138
Identity = 247/272 (90.81%), Postives = 259/272 (95.22%), Query Frame = 0

Query: 71  MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISP 130
           MNQIL  LRYWLVNHPIIL+FEWIQGQTF STPLFLILTVFSYLFLT LLSHIP+PSISP
Sbjct: 1   MNQILPDLRYWLVNHPIILDFEWIQGQTFGSTPLFLILTVFSYLFLTLLLSHIPLPSISP 60

Query: 131 HFLKPISALHNFVLLILSFIMALGCTLSSIYHVPHLYWIVCFPPRTPPVGPLFFWAYVFY 190
           HFLKPISALHNFVLL LSFIMALGC LSSIYHVPHL+W++CFPPRTPPVGPLFFWAYVFY
Sbjct: 61  HFLKPISALHNFVLLSLSFIMALGCALSSIYHVPHLHWLICFPPRTPPVGPLFFWAYVFY 120

Query: 191 LSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTNATVHV 250
           LSKILEF+DTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIAL+TNA VHV
Sbjct: 121 LSKILEFVDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALLTNAVVHV 180

Query: 251 IMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCSGFWGWCFN 310
           IMYGYYFLCTFGIRP+WKRLVTDCQILQF+FSFV+SGQMLYEHFGGS  GCSG  GWCFN
Sbjct: 181 IMYGYYFLCTFGIRPRWKRLVTDCQILQFIFSFVISGQMLYEHFGGSGGGCSGIRGWCFN 240

Query: 311 AVFNGSLLALFINFHLKSYAANRKKKRIESKT 343
           AVFN SLLALFINFHLKSYAANR KKR+E+KT
Sbjct: 241 AVFNASLLALFINFHLKSYAANR-KKRMETKT 271

BLAST of Lsi03G008650 vs. TAIR 10
Match: AT3G06470.1 (GNS1/SUR4 membrane protein family )

HSP 1 Score: 322.0 bits (824), Expect = 5.8e-88
Identity = 173/279 (62.01%), Postives = 201/279 (72.04%), Query Frame = 0

Query: 71  MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHI--PIPSI 130
           M  I S L YWLVNHP I NF WI+G+T  ST  F+ + V  YL  TFLL      +PS+
Sbjct: 1   MASIYSSLTYWLVNHPYISNFTWIEGETLGSTVFFVSVVVSVYLSATFLLRSAIDSLPSL 60

Query: 131 SPHFLKPISALHNFVLLILSFIMALGCTLS------SIYHVPHLYWIVCFPPRTPPVGPL 190
           SP  LKPI+A+H+ +L +LS +MA+GCTLS      S   +      +CFP    P GPL
Sbjct: 61  SPRILKPITAVHSLILCLLSLVMAVGCTLSITSSHASSDPMARFLHAICFPVDVKPNGPL 120

Query: 191 FFWAYVFYLSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIAL 250
           FFWA VFYLSKILEF DT+LIIL  S QRL+FLHVYHH+TV++MCYLWL T QS+FPIAL
Sbjct: 121 FFWAQVFYLSKILEFGDTILIILGKSIQRLSFLHVYHHATVVVMCYLWLRTRQSMFPIAL 180

Query: 251 VTNATVHVIMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGGSVAGCS 310
           VTN+TVHVIMYGYYFLC  G RPKWKRLVTDCQI+QFVFSF +SG ML EH  GS  GC+
Sbjct: 181 VTNSTVHVIMYGYYFLCAVGSRPKWKRLVTDCQIVQFVFSFGLSGWMLREHLFGS--GCT 240

Query: 311 GFWGWCFNAVFNGSLLALFINFHLKSYAANRKKKRIESK 342
           G WGWCFNA FN SLLALF NFH K+Y   +K  R + K
Sbjct: 241 GIWGWCFNAAFNASLLALFSNFHSKNYV--KKPTREDGK 275

BLAST of Lsi03G008650 vs. TAIR 10
Match: AT3G06460.1 (GNS1/SUR4 membrane protein family )

HSP 1 Score: 285.0 bits (728), Expect = 7.8e-77
Identity = 152/265 (57.36%), Postives = 186/265 (70.19%), Query Frame = 0

Query: 71  MNQILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHI--PIPSI 130
           M  + S L YWLV+HP I NF W +G+T  ST  F+ + V  YL  TFLL +    +P++
Sbjct: 1   MASVYSTLTYWLVHHPYIANFTWTEGETLGSTVFFVFVVVSLYLSATFLLRYTVDSLPTL 60

Query: 131 SPHFLKPISALHNFVLLILSFIMALGCTLSSIYH---VPHLYWIVCFPPRTPPVGPLFFW 190
            P  LKPI+A+H+ +L +LS  MA+GCTLS I        L+  VCFP    P GPLFFW
Sbjct: 61  GPRILKPITAVHSLILFLLSLTMAVGCTLSLISSSDPKARLFDAVCFPLDVKPKGPLFFW 120

Query: 191 AYVFYLSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQSLFPIALVTN 250
           A VFYLSKILEF+DTLLIIL  S QRL+FLHVYHH+TV+I+CYLWL T QS+FP+ LV N
Sbjct: 121 AQVFYLSKILEFVDTLLIILNKSIQRLSFLHVYHHATVVILCYLWLRTRQSMFPVGLVLN 180

Query: 251 ATVHVIMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSF-VVSGQMLYEHFGGSVAGCSGF 310
           +TVHVIMYGYYFLC  G RPKWK+LVT+ Q++QF F   + +  ML EH+ GS  GC+G 
Sbjct: 181 STVHVIMYGYYFLCAIGSRPKWKKLVTNFQMVQFAFGMGLGAAWMLPEHYFGS--GCAGI 240

Query: 311 WGWCFNAVFNGSLLALFINFHLKSY 330
           W   FN VF  SLLALF NFH K+Y
Sbjct: 241 WTVYFNGVFTASLLALFYNFHSKNY 263

BLAST of Lsi03G008650 vs. TAIR 10
Match: AT1G75000.1 (GNS1/SUR4 membrane protein family )

HSP 1 Score: 117.9 bits (294), Expect = 1.7e-26
Identity = 81/272 (29.78%), Postives = 132/272 (48.53%), Query Frame = 0

Query: 75  LSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSIS----- 134
           ++   Y+L  HP I+NF W   Q++AST  FL   V SY+     L  + + ++S     
Sbjct: 1   MTTFNYYLAEHPTIVNFRWSPTQSYASTWSFLFTAVSSYIIAAVTLHLLLLITLSLSNRR 60

Query: 135 -PHFLKPISALHNFVLLILSFIMALGCTLSSIYHV------------PHLYWIVCFPPRT 194
               L PI ALH+  + I+S ++ +G  LS+   +              L W +CFP  T
Sbjct: 61  RGFSLGPIPALHSLTISIISAVIFVGILLSAAAEIRDTRWLWRRTRTTALQWFLCFPVGT 120

Query: 195 PPVGPLFFWAYVFYLSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQS 254
              G +FFW+Y FYLS+ L    T   ++    ++L+F  + + S++L + +LWL  SQS
Sbjct: 121 RASGRVFFWSYAFYLSRFLHLFRTFFSVIRR--RKLSFFQLINQSSLLCISFLWLEYSQS 180

Query: 255 LFPIALVTNATVHVIMYGYYFLCTFGIRPKWKRLVTDCQILQFVFSFVVSGQMLYEHFGG 314
              +A++     + ++YGY F    G+R      V +CQ +      V    +L  H   
Sbjct: 181 FQVVAILLTTVSYAVVYGYRFWTEIGLRGACFPFVGNCQAILLGCMTVCHVGVLCIHL-V 240

Query: 315 SVAGCSGFWGWCFNAVFNGSLLALFINFHLKS 329
              GC+G   W FN+V N  +  L++ F+ K+
Sbjct: 241 KRGGCNGIGAWLFNSVLNAVITLLYLKFYCKT 269

BLAST of Lsi03G008650 vs. TAIR 10
Match: AT4G36830.1 (GNS1/SUR4 membrane protein family )

HSP 1 Score: 114.0 bits (284), Expect = 2.4e-25
Identity = 83/280 (29.64%), Postives = 145/280 (51.79%), Query Frame = 0

Query: 74  ILSVLRYWLVNHPIILNFEWIQGQTFASTPLFLILTVFSYLFLTFLLSHIPIPSISPHF- 133
           +++ + Y+L  HP I+ F W   Q++ ST  FL  ++  Y+ ++  L HI + ++     
Sbjct: 5   LINSITYFLSEHPYIVGFRWSNSQSWGSTWSFLFTSISLYIAVSSSL-HILLSAVRRSNR 64

Query: 134 ---LKPISALHNFVLLILSFIMALGCTLSSIYHV-------------PHLYWIVCFPPRT 193
              L  I  +H+ ++ ILS  +  G  LS+   +               L W++CFP  T
Sbjct: 65  SVPLGHIPEIHSLLMSILSATIFAGILLSAAAEIRDTRWLWRRSKTATPLQWLLCFPLGT 124

Query: 194 PPVGPLFFWAYVFYLSKILEFIDTLLIILTGSFQRLTFLHVYHHSTVLIMCYLWLHTSQS 253
            P G +FFW+YVFYL++ L    T+  +L    +RL    ++ +S +    +LWL  SQS
Sbjct: 125 RPSGRVFFWSYVFYLTRFLHMFRTIFAVLRS--RRLAVSQLFCNSVMAFTSFLWLEFSQS 184

Query: 254 LFPIALVTNATVHVIMYGYYFLCTFGI-RPKWKRLVTDCQILQFVFSFVVSGQMLYEH-F 313
              +A+++   V+ ++YGY F   FG+    +   V +CQ++    + V    +L  H F
Sbjct: 185 YQILAILSTTLVYSVVYGYRFWTGFGLPGSAFPSFVVNCQLVLVGCNLVSHAGVLTMHLF 244

Query: 314 GGSVAGCSGFWGWCFNAVFNGSLLALFINFHLKSYAANRK 335
            G   GC+G   W  N+V NG++L LF+NF+++ ++  R+
Sbjct: 245 KG---GCNGIGAWGLNSVLNGAILLLFLNFYVRMHSPMRR 278

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SYY43.4e-2429.64Elongation of fatty acids protein 3-like OS=Arabidopsis thaliana OX=3702 GN=HOS3... [more]
Q54CJ43.5e-2136.15Elongation of fatty acids protein A OS=Dictyostelium discoideum OX=44689 GN=eloA... [more]
Q86JM58.0e-1831.02Putative elongation of fatty acids protein DDB_G0272012 OS=Dictyostelium discoid... [more]
P395401.0e-1733.08Elongation of fatty acids protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204... [more]
P403194.0e-1730.62Elongation of fatty acids protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204... [more]
Match NameE-valueIdentityDescription
A0A0A0KJH85.2e-14595.97Very-long-chain 3-oxoacyl-CoA synthase OS=Cucumis sativus OX=3659 GN=Csa_6G41130... [more]
A0A5A7UQE38.9e-14595.60Very-long-chain 3-oxoacyl-CoA synthase OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S4DXJ28.9e-14595.60Very-long-chain 3-oxoacyl-CoA synthase OS=Cucumis melo OX=3656 GN=LOC103491307 P... [more]
A0A6J1EPN22.1e-13890.81Very-long-chain 3-oxoacyl-CoA synthase OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1JN461.0e-13790.81Very-long-chain 3-oxoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC1114867... [more]
Match NameE-valueIdentityDescription
XP_038886924.13.3e-14696.70elongation of fatty acids protein 3-like [Benincasa hispida][more]
XP_004140108.11.1e-14495.97elongation of fatty acids protein 3-like [Cucumis sativus] >KGN47926.1 hypotheti... [more]
XP_016900704.11.8e-14495.60PREDICTED: elongation of fatty acids protein 3-like [Cucumis melo] >KAA0057330.1... [more]
XP_023529341.13.3e-13891.54elongation of fatty acids protein 3-like [Cucurbita pepo subsp. pepo][more]
XP_022927675.14.4e-13890.81elongation of fatty acids protein 3-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G06470.15.8e-8862.01GNS1/SUR4 membrane protein family [more]
AT3G06460.17.8e-7757.36GNS1/SUR4 membrane protein family [more]
AT1G75000.11.7e-2629.78GNS1/SUR4 membrane protein family [more]
AT4G36830.12.4e-2529.64GNS1/SUR4 membrane protein family [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002076ELO familyPFAMPF01151ELOcoord: 99..336
e-value: 8.2E-51
score: 172.9
IPR002076ELO familyPANTHERPTHR11157FATTY ACID ACYL TRANSFERASE-RELATEDcoord: 70..335
NoneNo IPR availablePANTHERPTHR11157:SF132ELONGATION OF FATTY ACIDS PROTEIN 3-LIKEcoord: 70..335
IPR030457ELO family, conserved sitePROSITEPS01188ELOcoord: 212..220

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G008650.1Lsi03G008650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034625 fatty acid elongation, monounsaturated fatty acid
biological_process GO:0034626 fatty acid elongation, polyunsaturated fatty acid
biological_process GO:0019367 fatty acid elongation, saturated fatty acid
biological_process GO:0030148 sphingolipid biosynthetic process
biological_process GO:0042761 very long-chain fatty acid biosynthetic process
cellular_component GO:0030176 integral component of endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009922 fatty acid elongase activity
molecular_function GO:0102756 very-long-chain 3-ketoacyl-CoA synthase activity