Lsi03G008630 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G008630
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pectin lyase-like superfamily protein
Location	chr03: 14354654 .. 14356213 (+)
RNA-Seq Expression	Lsi03G008630
Synteny	Lsi03G008630

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTATCCTTACAACCCTTTTATTGCTTTACTTTTCTTGTATGTTTCTCAACTCAAAATGTGTCATTGCATTGTCATATAATATTGTTAGTTTTGGAGCCAAGGCTGATGGCAAATCTGATTCAACACAAGCATTTCAAAGGGCATGGGCCAATGCATGCGGCTCCATTAAACCAGCTTCCATTTATGTGCCTCAAGGAAGATTTTACCTTCAAAGTGGAACATTCAATGGACATTGCAAGAATAATGCTATTTTCATACGTATTGATGGCACTCTAGTGGCCCCCTCTGATTATCGAGTTATTGGCAATTCTGCAGCTTGGATCATGTTTAAGAATGTTGATGGAGTAACTATCTCAGGTGGCATTCTTGATGGTCAAGGAACAAGCTTATGGGCTTGTAAAAATGCTGCCAAGACTTGCCCAACTGGTGCTTCGGTATGTAAATTAATTAATTAATCAATCAATAAGACAATATTTTACTAAGTTTATAATTAACTAATTATATTCTCTTTAAGACAAGTTCATGTTTTTATTTTTTAAGCATTTGGTAGAAGTATCCATGTTCTTACTCAGAAAACTATTGGGTGTAGCCATAACATCCATTTTCATTTCTCTTGCATTAATTTTTTTTTTTTTTTTTACTTTTATGTAGACGCTCGAATTTTCCTACTCAAAAAATGTAGTGATTAGTGCATTAACATCACTCAAAAGTCAAATGTTCCACATTGTGATCAATCATTGCCAAAATATGAAAATGAAAGGACTTAAGGTCTTTGCCTCTGGTGATAGCCCCAACACTGATGGTATACACGTACAACTATCATCCAATGTGACTATACTAAACTCTAAGATAGGCACGGAGATGACTGTATATCCATCGGTCCTGGCACCTTCAACCTATGGATGGAGAATATTATTTGCGTACCTGGTCATGGAATTAGGTGCGTCTTATTATCATGATATTACAGATTTGTAAATACAAAAGGAAAATGAAAAATTTATCATTGTGTTAATGTATTTGGTTGGTTTTGTAGCATTGGAAGTCTAGCAAAGGATCCAGAAGAGGCTGGGGTGCAAAATATTACCGTGAAAAATGCTTTATTTTCACACTCAAAACGGGGTGAGAATCAAGGCATGGGTCAGGCCAAGCAATGGATTTGTTAGGAACATTCTCTTCAAAAACATAGTAATGGATAATGTGCAAAACCCAATTCTTATTGATCAAAACTATTGTCCAAGTCATACAGGATGTCCGGACAAGGTATAAACTTAATCAATTAATATGACTTCTCTAGTTAAGAGACTTTTTTTAATTTTCTTATTGTTGGTATGGAACAGGCTTCTGGAATTAAAATTAGCGATGTGACATACAAAAACATTCATGGAACATCAACCACTAAAGTTGCAGTAAAATTTGATTGCAGTCCTACAAACCAATGCAACCAAATAAAGTTGGAGGATGTAAAGCTTAGTTATATGGATCAATTAGCTCAATCAAGATGTAGCAATGTTGGGGGGATTGCTACAGGTCTTGTTCAGCCTACTAGTTGCTTCTAG

mRNA sequence

ATGACTATCCTTACAACCCTTTTATTGCTTTACTTTTCTTGTATGTTTCTCAACTCAAAATGTGTCATTGCATTGTCATATAATATTGTTAGTTTTGGAGCCAAGGCTGATGGCAAATCTGATTCAACACAAGCATTTCAAAGGGCATGGGCCAATGCATGCGGCTCCATTAAACCAGCTTCCATTTATGTGCCTCAAGGAAGATTTTACCTTCAAAGTGGAACATTCAATGGACATTGCAAGAATAATGCTATTTTCATACGTATTGATGGCACTCTAGTGGCCCCCTCTGATTATCGAGTTATTGGCAATTCTGCAGCTTGGATCATGTTTAAGAATGTTGATGGAGTAACTATCTCAGGTGGCATTCTTGATGGTCAAGGAACAAGCTTATGGGCTTGTAAAAATGCTGCCAAGACTTGCCCAACTGGTGCTTCGGTATCATTTGGTAGAAGTATCCATGTTCTTACTCAGAAAACTATTGGGCACGGAGATGACTGTATATCCATCGGTCCTGGCACCTTCAACCTATGGATGGAGAATATTATTTGCGTACCTGGTCATGGAATTAGCATTGGAAGTCTAGCAAAGGATCCAGAAGAGGCTGGGGTGCAAAATATTACCGTGAAAAATGCTTTATTTTCACACTCAAAACGGGGTGAGAATCAAGGCATGGGATGTCCGGACAAGGCTTCTGGAATTAAAATTAGCGATGTGACATACAAAAACATTCATGGAACATCAACCACTAAAGTTGCAGTAAAATTTGATTGCAGTCCTACAAACCAATGCAACCAAATAAAGTTGGAGGATGTAAAGCTTAGTTATATGGATCAATTAGCTCAATCAAGATGTAGCAATGTTGGGGGGATTGCTACAGGTCTTGTTCAGCCTACTAGTTGCTTCTAG

Coding sequence (CDS)

ATGACTATCCTTACAACCCTTTTATTGCTTTACTTTTCTTGTATGTTTCTCAACTCAAAATGTGTCATTGCATTGTCATATAATATTGTTAGTTTTGGAGCCAAGGCTGATGGCAAATCTGATTCAACACAAGCATTTCAAAGGGCATGGGCCAATGCATGCGGCTCCATTAAACCAGCTTCCATTTATGTGCCTCAAGGAAGATTTTACCTTCAAAGTGGAACATTCAATGGACATTGCAAGAATAATGCTATTTTCATACGTATTGATGGCACTCTAGTGGCCCCCTCTGATTATCGAGTTATTGGCAATTCTGCAGCTTGGATCATGTTTAAGAATGTTGATGGAGTAACTATCTCAGGTGGCATTCTTGATGGTCAAGGAACAAGCTTATGGGCTTGTAAAAATGCTGCCAAGACTTGCCCAACTGGTGCTTCGGTATCATTTGGTAGAAGTATCCATGTTCTTACTCAGAAAACTATTGGGCACGGAGATGACTGTATATCCATCGGTCCTGGCACCTTCAACCTATGGATGGAGAATATTATTTGCGTACCTGGTCATGGAATTAGCATTGGAAGTCTAGCAAAGGATCCAGAAGAGGCTGGGGTGCAAAATATTACCGTGAAAAATGCTTTATTTTCACACTCAAAACGGGGTGAGAATCAAGGCATGGGATGTCCGGACAAGGCTTCTGGAATTAAAATTAGCGATGTGACATACAAAAACATTCATGGAACATCAACCACTAAAGTTGCAGTAAAATTTGATTGCAGTCCTACAAACCAATGCAACCAAATAAAGTTGGAGGATGTAAAGCTTAGTTATATGGATCAATTAGCTCAATCAAGATGTAGCAATGTTGGGGGGATTGCTACAGGTCTTGTTCAGCCTACTAGTTGCTTCTAG

Protein sequence

MTILTTLLLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPASIYVPQGRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTISGGILDGQGTSLWACKNAAKTCPTGASVSFGRSIHVLTQKTIGHGDDCISIGPGTFNLWMENIICVPGHGISIGSLAKDPEEAGVQNITVKNALFSHSKRGENQGMGCPDKASGIKISDVTYKNIHGTSTTKVAVKFDCSPTNQCNQIKLEDVKLSYMDQLAQSRCSNVGGIATGLVQPTSCF

Homology

[Close]

BLAST of Lsi03G008630 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.2e-89
Identity = 181/375 (48.27%), Postives = 219/375 (58.40%), Query Frame = 0


Query: 17  LNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPASIYVPQGRFYLQSGTF 76
           +NS     L+YN+ S GAKADGK+DST+AF  AWA AC S+ P  IYVP G F+L+   F
Sbjct: 18  INSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPAGTFFLRDVVF 77

Query: 77  NGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTISGGILDGQGTSLWACKN 136
           +G CKNNAI  RI GTLVAPSDYRVIGN+A WI F +V+GVTISGGILDGQGT+LWACK 
Sbjct: 78  SGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGILDGQGTALWACKA 137

Query: 137 A-AKTCPTGAS-------------------------------------VSFGRS------ 196
              ++CP+GA+                                     V   RS      
Sbjct: 138 CHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQGVRVSRSGNSPNT 197

Query: 197 --IHV-------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGHGISIGSLAKDPEEAG 256
             IHV       +    I  GDDC+SIGPGT NLW+E + C PGHGISIGSL K+ EEAG
Sbjct: 198 DGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGPGHGISIGSLGKEQEEAG 257

Query: 257 VQNITVKNALFSHSKRG---------------------------EN----------QGMG 302
           VQN+TVK   FS ++ G                           EN             G
Sbjct: 258 VQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVNVENPIVIDQHYCPDNKG 317

[Close]

BLAST of Lsi03G008630 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 7.4e-60
Identity = 149/397 (37.53%), Postives = 199/397 (50.13%), Query Frame = 0


Query: 1   MTILTTLLLLYFSCMFLNSKCVIALSY------NIVSFGAKADGKSDSTQAFQRAWANAC 60
           M   T+ L+L  + + L+   + +L++      N++S+GAK DG  DST+AF  AW  AC
Sbjct: 1   MVYNTSCLILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSKDSTKAFLAAWDVAC 60

Query: 61  GSIKPASIYVPQGRFYLQSGTFNGH-CKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKN 120
            S  P +I VP+GRF + +  F+G+ CK   I IRI G++VAP D+R+I +S  WI F++
Sbjct: 61  ASANPTTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFED 120

Query: 121 VDGVTISGGILDGQGTSLWACK-NAAKTCPTGA-SVSFGRS------------------- 180
           V  V+I GGILD QGTSLW CK N    CPTGA S+ F  S                   
Sbjct: 121 VTDVSIYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIV 180

Query: 181 -------------------------IHV-------LTQKTIGHGDDCISIGPGTFNLWME 240
                                    IHV       +T   IG GDDCISIGPG+ N++++
Sbjct: 181 IDNSNNVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQ 240

Query: 241 NIICVPGHGISIGSLAKDPEEAGVQNITVKNALFSHSKRGE------------------- 300
            I C PGHGISIGSL +  EE GV N+TV N  F  +  G                    
Sbjct: 241 TIRCGPGHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQ 300

Query: 301 --NQGM---------------GCPDKASGIKISDVTYKNIHGTSTTKVAVKFDCSPTNQC 302
             N  M                CP + SG+K+S+V Y++IHGTS T+VAV  DCS    C
Sbjct: 301 HINMKMVKNPIIIDQHYCLHKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPC 360

[Close]

BLAST of Lsi03G008630 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.1e-38
Identity = 112/372 (30.11%), Postives = 162/372 (43.55%), Query Frame = 0


Query: 26  SYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPASIYVPQGRFYLQSGTFNGHCKNNAI 85
           S++I   GA  +GK+DST+A Q AWA+ACG     +I +P+G F +    F G CK + +
Sbjct: 40  SFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCKGD-V 99

Query: 86  FIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTISG-GILDGQGTSLWA----------- 145
            I+++G L+A +D     +   WI    VD + I+G G LDGQG ++W+           
Sbjct: 100 TIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYDCK 159

Query: 146 ---------------------------------CKN---------AAKTCPTGASVSFGR 205
                                            CK+         A    P    +  G 
Sbjct: 160 ILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGD 219

Query: 206 SIHV-LTQKTIGHGDDCISIGPGTFNLWMENIICVPGHGISIGSLAKDPEEAGVQNITVK 265
           S  V +T   IG GDDCISIGPGT  + +  + C PGHGISIGSL +  +E  V +I VK
Sbjct: 220 SSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVK 279

Query: 266 NALFSHSKRG-------------------------ENQGMG-------CPDK------AS 303
           +     +  G                         E+ G         CP+K      AS
Sbjct: 280 DCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCTANGAS 339

[Close]

BLAST of Lsi03G008630 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 3.6e-38
Identity = 112/372 (30.11%), Postives = 162/372 (43.55%), Query Frame = 0


Query: 26  SYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPASIYVPQGRFYLQSGTFNGHCKNNAI 85
           S++I   GA  +GK+DST+A Q AWA+ACG     +I +P+G F +    F G CK + +
Sbjct: 40  SFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGD-V 99

Query: 86  FIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTISG-GILDGQGTSLWA----------- 145
            I++DG L+A +D     +   WI    VD + I+G G LDGQG ++W+           
Sbjct: 100 TIQVDGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCK 159

Query: 146 ---------------------------------CKN---------AAKTCPTGASVSFGR 205
                                            CK+         A    P    +  G 
Sbjct: 160 ILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMGD 219

Query: 206 SIHV-LTQKTIGHGDDCISIGPGTFNLWMENIICVPGHGISIGSLAKDPEEAGVQNITVK 265
           S  + +T   IG GDDCISIGPGT  + +  + C PGHGISIGSL +  +E  V +I VK
Sbjct: 220 SSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVK 279

Query: 266 NALFSHSKRG--------------------ENQGMG------------CPDK------AS 303
           +     +  G                    EN  M             CP+K      AS
Sbjct: 280 DCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGAS 339

[Close]

BLAST of Lsi03G008630 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 4.7e-38
Identity = 112/372 (30.11%), Postives = 160/372 (43.01%), Query Frame = 0


Query: 26  SYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPASIYVPQGRFYLQSGTFNGHCKNNAI 85
           S++I   GA  +GK+DST+A Q AWA+ACG     +I +P+G F +    F G CK + +
Sbjct: 40  SFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGD-V 99

Query: 86  FIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTISG-GILDGQGTSLWA----------- 145
            I++DG L+A +D         WI    VD + I+G G LDGQG ++W+           
Sbjct: 100 TIQVDGNLLATTDLSQYKEHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCK 159

Query: 146 ---------------------------------CKN---------AAKTCPTGASVSFGR 205
                                            CKN         A    P    +  G 
Sbjct: 160 ILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIKDVTVTAPGDSPNTDGIHMGD 219

Query: 206 SIHV-LTQKTIGHGDDCISIGPGTFNLWMENIICVPGHGISIGSLAKDPEEAGVQNITVK 265
           S  + +T   IG GDDCISIGPGT  + +  + C PGHGISIGSL +  +E  V +I VK
Sbjct: 220 SSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVK 279

Query: 266 NALFSHSKRG--------------------ENQGMG------------CPDK------AS 303
           +     +  G                    EN  M             CP+K      AS
Sbjct: 280 DCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGAS 339

[Close]

BLAST of Lsi03G008630 vs. ExPASy TrEMBL
Match: A0A6J1EHY1 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434520 PE=3 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 6.9e-109
Identity = 216/390 (55.38%), Postives = 252/390 (64.62%), Query Frame = 0


Query: 1   MTILTTLLLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPA 60
           M+IL   LLL+F  MFL S   I+ S N+VS GAK DGKSDSTQAF+ AWANACGS +P 
Sbjct: 1   MSILRGFLLLFFPFMFLYSSSAISFSNNVVSLGAKPDGKSDSTQAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNG CKNNAIFIRIDGTLVAPSDYRVIGNSAAWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRGVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GGI DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGIFDGKGSGLWACKNSTKTCPSGATTIEISDSKNVVLSALTSFNSQMFHIVVNGCQNIK 180

Query: 181 ------VSFGRS-----IHV-------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGH 240
                 ++ G S     IHV       +    IG GDDCISIGPGT NLW+ENI+C PGH
Sbjct: 181 MKGLKVLASGNSPNTDGIHVQLSSDVTILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

Query: 241 GISIGSLAKDPEEAGVQNITVKNALFSHSKRGEN-------------------------- 300
           GISIGSLAKD EEAGVQN+TVK ALFS ++ G                            
Sbjct: 241 GISIGSLAKDLEEAGVQNVTVKKALFSRTQNGVRIKAWGRPSNGFVKNILFQHIVMDNVQ 300

Query: 301 -----------QGMGCPDKASGIKISDVTYKNIHGTSTTKVAVKFDCSPTNQCNQIKLED 302
                         GCP +ASG+KISDVTY+NIHGTS T++AVKFDCSPTN C++IK+ED
Sbjct: 301 NPIVIDQNYCPSHKGCPGQASGVKISDVTYQNIHGTSATQIAVKFDCSPTNPCSRIKMED 360

[Close]

BLAST of Lsi03G008630 vs. ExPASy TrEMBL
Match: A0A6J1EJA0 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434653 PE=3 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 5.8e-108
Identity = 214/390 (54.87%), Postives = 251/390 (64.36%), Query Frame = 0


Query: 1   MTILTTLLLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPA 60
           MTIL   LL++F  MFLNS   IA S N+VS GAK DGKSDST+AF+ AWANACGSI+P 
Sbjct: 1   MTILRGFLLVFFPFMFLNSSSAIAFSNNVVSLGAKPDGKSDSTKAFETAWANACGSIRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNG CKNNAIFIRIDGTLVAPSDY+VIGNSAAWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYKVIGNSAAWILFRGVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GGI DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGIFDGKGSGLWACKNSTKTCPSGATTIEISNSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------VSFGRS-----IHV-------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGH 240
                 ++ G S     IHV       +    IG GDDCISIGPGT NLW+ENI+C PGH
Sbjct: 181 MKGLKVLASGNSPNTDGIHVQLSSDVIILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

Query: 241 GISIGSLAKDPEEAGVQNITVKNALFSHSKRGEN-------------------------- 300
           GISIGSLAKD +EAGVQN+TVK ALFS ++ G                            
Sbjct: 241 GISIGSLAKDLQEAGVQNVTVKKALFSRTQNGVRIKAWGRPSNGFVKNILFQHIVMDNVQ 300

Query: 301 -----------QGMGCPDKASGIKISDVTYKNIHGTSTTKVAVKFDCSPTNQCNQIKLED 302
                          CP KASG+KISDVTY++IHGTS T++AVKFDCSPTN C+ IK+ED
Sbjct: 301 NPIVIDQNYCPSHKDCPGKASGVKISDVTYQDIHGTSATQIAVKFDCSPTNPCSGIKMED 360

[Close]

BLAST of Lsi03G008630 vs. ExPASy TrEMBL
Match: A0A6J1EMB1 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434652 PE=3 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 4.2e-106
Identity = 211/390 (54.10%), Postives = 247/390 (63.33%), Query Frame = 0


Query: 1   MTILTTLLLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPA 60
           M+IL   LLL+F  MFL S   IA S N+VS GAK DGKSDST+AF+ AWANACGS +P 
Sbjct: 1   MSILRGFLLLFFPFMFLYSSSAIAFSNNVVSLGAKPDGKSDSTKAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNG CKNNAIFIRIDGTLVAPSDYRVIGNSAAWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRGVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GG+ DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGVFDGKGSGLWACKNSTKTCPSGATTIEISHSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------VSFGRS-----IHV-------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGH 240
                 ++ G S     IHV       +    IG GDDCISIGPGT NLW+ENI+C PGH
Sbjct: 181 IKGLKVLALGNSPNTDGIHVQLSSDVIILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

Query: 241 GISIGSLAKDPEEAGVQNITVKNALFSHSKRGEN-------------------------- 300
           GISIGSLAKD EEAGVQN+TVK  LFS ++ G                            
Sbjct: 241 GISIGSLAKDLEEAGVQNVTVKKTLFSRTQNGVRIKAWGRPSNGFVKNILFQHIVMDNVQ 300

Query: 301 -----------QGMGCPDKASGIKISDVTYKNIHGTSTTKVAVKFDCSPTNQCNQIKLED 302
                         GCP +ASG+KISDVTY++IHGTS T++AVKFDCSPTN C+ IK+ED
Sbjct: 301 NPIVIDQNYCPNHKGCPGQASGVKISDVTYQDIHGTSATQIAVKFDCSPTNPCSGIKMED 360

[Close]

BLAST of Lsi03G008630 vs. ExPASy TrEMBL
Match: A0A6J1JGG6 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486679 PE=3 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.9e-103
Identity = 206/390 (52.82%), Postives = 247/390 (63.33%), Query Frame = 0


Query: 1   MTILTTLLLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPA 60
           MTIL   LLL+F  +FL S   IA S N+V  GAK DGKSDST+AF+ AWANACGS +P 
Sbjct: 1   MTILRGFLLLFFPFVFLYSSSAIAFSNNVVDLGAKPDGKSDSTKAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNG CKNNAIFIRIDGTLVAPSDYRVIGNSAAWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSLTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRRVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GG+ DG+G+ LWACKN+ +TCP+GA+                                  
Sbjct: 121 GGVFDGKGSGLWACKNSTRTCPSGATTIEISNSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------VSFGRS-----IHV-------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGH 240
                 ++ G S     IHV       +    IG GDDCISIGPGT +LW+ENI+C PGH
Sbjct: 181 MKGLVVLASGNSPNTDGIHVQLSSDVTILNSKIGTGDDCISIGPGTSSLWVENIVCGPGH 240

Query: 241 GISIGSLAKDPEEAGVQNITVKNALFSHSKRGEN-------------------------- 300
           GISIGSLAKD EEAGVQN+T+K ALFS ++ G                            
Sbjct: 241 GISIGSLAKDLEEAGVQNVTIKKALFSRTQNGVRIKAWGRPSNGFVKNILFQHIVMDNVQ 300

Query: 301 -----------QGMGCPDKASGIKISDVTYKNIHGTSTTKVAVKFDCSPTNQCNQIKLED 302
                          CP +ASG+KIS VTY++IHGTS T++AVKFDCSPTN C+ IK+ED
Sbjct: 301 NPIVIDQNYCPSHKDCPGQASGVKISGVTYQDIHGTSATQIAVKFDCSPTNPCSGIKMED 360

[Close]

BLAST of Lsi03G008630 vs. ExPASy TrEMBL
Match: A0A6J1JPZ1 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486677 PE=3 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 9.6e-103
Identity = 206/390 (52.82%), Postives = 246/390 (63.08%), Query Frame = 0


Query: 1   MTILTTLLLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPA 60
           MTIL   LLL+F  +FL S   IA S N+V  GAK DGKSDST+AF+ AWANACGS +P 
Sbjct: 1   MTILRGFLLLFFPFVFLYSSSAIAFSNNVVDLGAKPDGKSDSTKAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNG CKNNAIFIRIDGTLVAPSDYRVIGNSAAWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSLTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRRVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GG+ DG+G+ LWACKN+ +TCP+GA+                                  
Sbjct: 121 GGVFDGKGSGLWACKNSTRTCPSGATTIEISNSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------VSFGRS-----IHV-------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGH 240
                 ++ G S     IHV       +    IG GDDCISIGPGT +LW+ENI+C PGH
Sbjct: 181 MKGLVVLASGNSPNTDGIHVQLSSDVTILNSKIGTGDDCISIGPGTSSLWIENIVCGPGH 240

Query: 241 GISIGSLAKDPEEAGVQNITVKNALFSHSKRGEN-------------------------- 300
           GISIGSLAKD EEAGVQN+T+K ALFS ++ G                            
Sbjct: 241 GISIGSLAKDLEEAGVQNVTIKKALFSRTQNGVRIKAWSRPSNGFVKSILFQHIVMDNVQ 300

Query: 301 -----------QGMGCPDKASGIKISDVTYKNIHGTSTTKVAVKFDCSPTNQCNQIKLED 302
                          CP +ASG+KIS VTY++IHGTS T++AVKFDCSPTN C+ IK+ D
Sbjct: 301 NPIVIDQNYCPSHKDCPGQASGVKISGVTYQDIHGTSATQIAVKFDCSPTNPCSGIKMGD 360

[Close]

BLAST of Lsi03G008630 vs. NCBI nr
Match: XP_038888748.1 (polygalacturonase-like, partial [Benincasa hispida])

HSP 1 Score: 423.7 bits (1088), Expect = 1.3e-114
Identity = 226/375 (60.27%), Postives = 246/375 (65.60%), Query Frame = 0


Query: 17  LNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPASIYVPQGRFYLQSGTF 76
           +N      LSYNIVSFGAKADGKSDS QAFQ+AW +ACGSIKPASIYVP+GRFYL+SG F
Sbjct: 3   INHAIKTPLSYNIVSFGAKADGKSDSIQAFQKAWDHACGSIKPASIYVPKGRFYLRSGKF 62

Query: 77  NGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTISGGILDGQGTSLWACKN 136
           NG CKN AIFIRIDGTLVAPSD+RVIGNSAAWIMFKNVDGVTI GGILDGQGT LWACK 
Sbjct: 63  NGPCKNKAIFIRIDGTLVAPSDFRVIGNSAAWIMFKNVDGVTILGGILDGQGTKLWACKT 122

Query: 137 AAKTCPTGAS-VSFGRS------------------------------------------- 196
               CPTGAS + F  S                                           
Sbjct: 123 TGNNCPTGASTIEFSYSKNVVISSLTSLKSQMFHIVINHCQNMKMKGLKIFASGDSPNTD 182

Query: 197 -IHV-------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGHGISIGSLAKDPEEAGV 256
            IHV       +    IG GDDCISIGPGTFNLW+ENIIC PGHGISIGSLAKD +EAGV
Sbjct: 183 GIHVQLSSDVTILNSKIGTGDDCISIGPGTFNLWIENIICGPGHGISIGSLAKDQQEAGV 242

Query: 257 QNITVKNALFSHSKRGEN-------------------------------------QGMGC 303
           QN+TVKNALFS ++ G                                       +   C
Sbjct: 243 QNVTVKNALFSRTQNGVRIKAWARASNGFVKNIIFKNIVMDNVYNPILIDQNYCPRHKEC 302

[Close]

BLAST of Lsi03G008630 vs. NCBI nr
Match: XP_022927702.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 403.7 bits (1036), Expect = 1.4e-108
Identity = 216/390 (55.38%), Postives = 252/390 (64.62%), Query Frame = 0


Query: 1   MTILTTLLLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPA 60
           M+IL   LLL+F  MFL S   I+ S N+VS GAK DGKSDSTQAF+ AWANACGS +P 
Sbjct: 1   MSILRGFLLLFFPFMFLYSSSAISFSNNVVSLGAKPDGKSDSTQAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNG CKNNAIFIRIDGTLVAPSDYRVIGNSAAWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRGVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GGI DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGIFDGKGSGLWACKNSTKTCPSGATTIEISDSKNVVLSALTSFNSQMFHIVVNGCQNIK 180

Query: 181 ------VSFGRS-----IHV-------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGH 240
                 ++ G S     IHV       +    IG GDDCISIGPGT NLW+ENI+C PGH
Sbjct: 181 MKGLKVLASGNSPNTDGIHVQLSSDVTILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

Query: 241 GISIGSLAKDPEEAGVQNITVKNALFSHSKRGEN-------------------------- 300
           GISIGSLAKD EEAGVQN+TVK ALFS ++ G                            
Sbjct: 241 GISIGSLAKDLEEAGVQNVTVKKALFSRTQNGVRIKAWGRPSNGFVKNILFQHIVMDNVQ 300

Query: 301 -----------QGMGCPDKASGIKISDVTYKNIHGTSTTKVAVKFDCSPTNQCNQIKLED 302
                         GCP +ASG+KISDVTY+NIHGTS T++AVKFDCSPTN C++IK+ED
Sbjct: 301 NPIVIDQNYCPSHKGCPGQASGVKISDVTYQNIHGTSATQIAVKFDCSPTNPCSRIKMED 360

[Close]

BLAST of Lsi03G008630 vs. NCBI nr
Match: XP_023531792.1 (polygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 401.7 bits (1031), Expect = 5.4e-108
Identity = 213/390 (54.62%), Postives = 250/390 (64.10%), Query Frame = 0


Query: 1   MTILTTLLLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPA 60
           MTIL   LLL+F  MFLNS   IA S N++S GAK DGKSDSTQAF+ AWANACGS +P 
Sbjct: 1   MTILRAFLLLFFPLMFLNSSGAIAFSNNVISLGAKPDGKSDSTQAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNG CKNNAIFIRIDGTLVAPSDY+VIGNSAAWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYKVIGNSAAWILFRRVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GG+ DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGVFDGKGSGLWACKNSTKTCPSGATTIEISNSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------VSFGRS-----IHV-------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGH 240
                 ++ G S     IHV       +    IG GDDCISIGPGT NLW+ENI+C PGH
Sbjct: 181 MKGLKVLASGNSPNTDGIHVQLSSDVTILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

Query: 241 GISIGSLAKDPEEAGVQNITVKNALFSHSKRGEN-------------------------- 300
           GISIGSLAKD EEAGVQN+TVK ALFS ++ G                            
Sbjct: 241 GISIGSLAKDLEEAGVQNVTVKKALFSRTQNGVRIKAWGRPSNGFVKNILFQHIVMDNVQ 300

Query: 301 -----------QGMGCPDKASGIKISDVTYKNIHGTSTTKVAVKFDCSPTNQCNQIKLED 302
                         GCP +ASG+KISDVTY++IHGTS T++AVKFDCSPTN C+ IK+ED
Sbjct: 301 NPIVIDQNYCPSHKGCPGQASGVKISDVTYQDIHGTSATQIAVKFDCSPTNPCSDIKMED 360

[Close]

BLAST of Lsi03G008630 vs. NCBI nr
Match: XP_022927889.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 400.6 bits (1028), Expect = 1.2e-107
Identity = 214/390 (54.87%), Postives = 251/390 (64.36%), Query Frame = 0


Query: 1   MTILTTLLLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPA 60
           MTIL   LL++F  MFLNS   IA S N+VS GAK DGKSDST+AF+ AWANACGSI+P 
Sbjct: 1   MTILRGFLLVFFPFMFLNSSSAIAFSNNVVSLGAKPDGKSDSTKAFETAWANACGSIRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNG CKNNAIFIRIDGTLVAPSDY+VIGNSAAWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYKVIGNSAAWILFRGVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GGI DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGIFDGKGSGLWACKNSTKTCPSGATTIEISNSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------VSFGRS-----IHV-------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGH 240
                 ++ G S     IHV       +    IG GDDCISIGPGT NLW+ENI+C PGH
Sbjct: 181 MKGLKVLASGNSPNTDGIHVQLSSDVIILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

Query: 241 GISIGSLAKDPEEAGVQNITVKNALFSHSKRGEN-------------------------- 300
           GISIGSLAKD +EAGVQN+TVK ALFS ++ G                            
Sbjct: 241 GISIGSLAKDLQEAGVQNVTVKKALFSRTQNGVRIKAWGRPSNGFVKNILFQHIVMDNVQ 300

Query: 301 -----------QGMGCPDKASGIKISDVTYKNIHGTSTTKVAVKFDCSPTNQCNQIKLED 302
                          CP KASG+KISDVTY++IHGTS T++AVKFDCSPTN C+ IK+ED
Sbjct: 301 NPIVIDQNYCPSHKDCPGKASGVKISDVTYQDIHGTSATQIAVKFDCSPTNPCSGIKMED 360

[Close]

BLAST of Lsi03G008630 vs. NCBI nr
Match: XP_022927888.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 394.4 bits (1012), Expect = 8.6e-106
Identity = 211/390 (54.10%), Postives = 247/390 (63.33%), Query Frame = 0


Query: 1   MTILTTLLLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPA 60
           M+IL   LLL+F  MFL S   IA S N+VS GAK DGKSDST+AF+ AWANACGS +P 
Sbjct: 1   MSILRGFLLLFFPFMFLYSSSAIAFSNNVVSLGAKPDGKSDSTKAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNG CKNNAIFIRIDGTLVAPSDYRVIGNSAAWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRGVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GG+ DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGVFDGKGSGLWACKNSTKTCPSGATTIEISHSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------VSFGRS-----IHV-------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGH 240
                 ++ G S     IHV       +    IG GDDCISIGPGT NLW+ENI+C PGH
Sbjct: 181 IKGLKVLALGNSPNTDGIHVQLSSDVIILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

Query: 241 GISIGSLAKDPEEAGVQNITVKNALFSHSKRGEN-------------------------- 300
           GISIGSLAKD EEAGVQN+TVK  LFS ++ G                            
Sbjct: 241 GISIGSLAKDLEEAGVQNVTVKKTLFSRTQNGVRIKAWGRPSNGFVKNILFQHIVMDNVQ 300

Query: 301 -----------QGMGCPDKASGIKISDVTYKNIHGTSTTKVAVKFDCSPTNQCNQIKLED 302
                         GCP +ASG+KISDVTY++IHGTS T++AVKFDCSPTN C+ IK+ED
Sbjct: 301 NPIVIDQNYCPNHKGCPGQASGVKISDVTYQDIHGTSATQIAVKFDCSPTNPCSGIKMED 360

[Close]

BLAST of Lsi03G008630 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 2.0e-76
Identity = 160/383 (41.78%), Postives = 208/383 (54.31%), Query Frame = 0


Query: 9   LLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPASIYVPQGR 68
           LL      L+     A +YNI+S+GAK DGK+DST+AF   WA AC S+KP +I VP+GR
Sbjct: 5   LLIVLLFLLSVSSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVPKGR 64

Query: 69  FYLQSGTFNG-HCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTISGGILDGQ 128
           F L+S  F+G  CK  ++  RI GTLVAPSDYRVIG    WI+F+++DG+++ GG+LD Q
Sbjct: 65  FLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVLDAQ 124

Query: 129 GTSLWACKNAAKTCPTGA-SVSFGRS---------------------------------- 188
           G SLW+CK + K CP+GA S+ F  S                                  
Sbjct: 125 GASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNVKLDGVKVS 184

Query: 189 ----------IHVLTQKT-------IGHGDDCISIGPGTFNLWMENIICVPGHGISIGSL 248
                     IHV +  T       I  GDDC+SIGPGT  LW+EN+ C PGHGISIGSL
Sbjct: 185 ADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSL 244

Query: 249 AKDPEEAGVQNITVKNALFSHSKRG----------------------------------E 302
            K+  E GVQNITVK A F+ ++ G                                  +
Sbjct: 245 GKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNPIVIDQ 304

[Close]

BLAST of Lsi03G008630 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 282.7 bits (722), Expect = 3.4e-76
Identity = 161/380 (42.37%), Postives = 207/380 (54.47%), Query Frame = 0


Query: 8   LLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPASIYVPQG 67
           LL+ F   F++S    A SYN++SFGAK DGK+D+T+AF   W  AC S +P +I VP+G
Sbjct: 4   LLVLFVFFFISS--CSAQSYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKG 63

Query: 68  RFYLQSGTFNG-HCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTISGGILDG 127
           RF L+S TF+G  CK   +  RIDGTLVAP+DYRVIGN   WI F+++DG+T+ GG+LD 
Sbjct: 64  RFLLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDA 123

Query: 128 QGTSLWACKNAAKTCPTGAS---------------------------------------- 187
           +G SLW CK + K CP+GA+                                        
Sbjct: 124 RGASLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKV 183

Query: 188 VSFGRS-----IHVLTQKT-------IGHGDDCISIGPGTFNLWMENIICVPGHGISIGS 247
           ++ G S     IHV +  +       I  GDDC+SIGPGT  LW+EN+ C PGHGISIGS
Sbjct: 184 LAAGNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGS 243

Query: 248 LAKDPEEAGVQNITVKNALFSHSKRG---------------------------EN----- 299
           L KD  E+GVQN+TVK   F+ +  G                           EN     
Sbjct: 244 LGKDSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIID 303

[Close]

BLAST of Lsi03G008630 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 4.8e-70
Identity = 149/387 (38.50%), Postives = 199/387 (51.42%), Query Frame = 0


Query: 4   LTTLLLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPASIY 63
           L  +LL++FS   L      A +YN+VSFGAK DG++DST+AF  AW  AC S    ++ 
Sbjct: 6   LVAVLLMFFSSFLLMKSSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAAAVTVT 65

Query: 64  VPQGRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTISGGI 123
           VP+G F L+   F G C++   F +I GT+VAPSDYR +GNS  WI+F  V+ ++I GG 
Sbjct: 66  VPRGSFLLKPVEFRGPCRSRITF-QIYGTIVAPSDYRGLGNSGYWILFVKVNRISIIGGT 125

Query: 124 LDGQGTSLWACKNAAKTCPTGA-SVSFG-------------------------------- 183
           LD +G S WAC+ + K+CP GA S++F                                 
Sbjct: 126 LDARGASFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLVINSCNNVIVRK 185

Query: 184 ------------RSIHV-------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGHGIS 243
                         +HV       +T  T   GDDCISIGPGT NL+M  + C PGHGIS
Sbjct: 186 VKLVAPDQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKLNCGPGHGIS 245

Query: 244 IGSLAKDPEEAGVQNITVKNALFSHSKRGEN----------------------------- 302
           IGSL +D  EAGV+NIT+ N++FS S  G                               
Sbjct: 246 IGSLGRDANEAGVENITLINSVFSGSDNGVRIKTWARQSTGFVRNVLFQNLIMKNVQNPI 305

[Close]

BLAST of Lsi03G008630 vs. TAIR 10
Match: AT1G05650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 236.5 bits (602), Expect = 2.8e-62
Identity = 137/386 (35.49%), Postives = 192/386 (49.74%), Query Frame = 0


Query: 6   TLLLLYFSCMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPASIYVP 65
           T  L++    F++     ++ +N+VSFGAK DG +DST AF +AW  ACGS   A++ VP
Sbjct: 7   TFPLIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASATVVVP 66

Query: 66  QGRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTISGGILD 125
            G F L+  TF G CK+   F ++ GT+VAP DYR  GNS +WI+F  V+  ++ GG  D
Sbjct: 67  TGTFLLKVITFGGPCKSKITF-QVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLVGGTFD 126

Query: 126 GQGTSLWACKNAAKTCPTGA-SVSFG------------------------------RSIH 185
            +G+  W+C+ + + CP G  S+SF                               R+I 
Sbjct: 127 ARGSGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVSHMTLNGCTNVAVRNIR 186

Query: 186 V---------------------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGHGISIG 245
           +                     LT  T+  GDDC++IG GT N  +  + C PGHG+SIG
Sbjct: 187 LVAPGDSPNTDGFTVQFSTGVTLTGSTVQTGDDCVAIGQGTRNFLISKLACGPGHGVSIG 246

Query: 246 SLAKDPEEAGVQNITVKNALFSHSKRGEN------------------------------- 302
           SLAK   E GV+N+TV +++F+ S+ G                                 
Sbjct: 247 SLAKQLNEDGVENVTVSSSVFTGSQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQNPIII 306

[Close]

BLAST of Lsi03G008630 vs. TAIR 10
Match: AT2G43880.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 233.8 bits (595), Expect = 1.8e-61
Identity = 133/384 (34.64%), Postives = 201/384 (52.34%), Query Frame = 0


Query: 9   LLYFS--CMFLNSKCVIALSYNIVSFGAKADGKSDSTQAFQRAWANACGSIKPASIYVPQ 68
           +++FS    FL        S+N+  +GA+ DG++D+T++F  AW+ ACGS   A +YVP+
Sbjct: 11  IIFFSINIFFLIKSSHAMPSFNVQRYGARGDGRADATKSFLTAWSLACGSRARAMVYVPR 70

Query: 69  GRFYLQSGTFNGHCKNNAIFIRIDGTLVAPSDYRVIGNSAAWIMFKNVDGVTISGGILDG 128
           G + +++  F G CK N I  + DGTLVAP++Y  IGNS  WI+F  V+ +++ GG +D 
Sbjct: 71  GTYLVKNLVFWGPCK-NIITFKNDGTLVAPANYWDIGNSGYWILFAKVNRISVYGGTIDA 130

Query: 129 QGTSLWACKNAAKTCPTGA-SVSF---------------GRSIHV--------------- 188
           +G   W+C+     CP GA S+SF                +++HV               
Sbjct: 131 RGAGYWSCRKKGSHCPQGARSISFSWCNNVLLSGLSSFNSQNMHVTVHHSSNVRIENVRI 190

Query: 189 ---------------------LTQKTIGHGDDCISIGPGTFNLWMENIICVPGHGISIGS 248
                                ++  TI  GDDCI++  G+ N+W+E + C PGHGISIGS
Sbjct: 191 RAPSGSPNTDGIHVQSSSGVTISGGTIATGDDCIALSQGSRNIWIERVNCGPGHGISIGS 250

Query: 249 LAKDPEEAGVQNITVKNALFSHSKRG---------------------------EN----- 302
           L     E GVQN+TV +++F+ ++ G                           EN     
Sbjct: 251 LGDYANEEGVQNVTVTSSVFTKTQNGVRIKTWARPSRGFVNNVVFRNLIMNNVENPVIID 310

The following BLAST results are available for this feature:

BLAST of Lsi03G008630 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P48979	1.2e-89	48.27	Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1	[more]
O22818	7.4e-60	37.53	Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386...	[more]
P35339	2.1e-38	30.11	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1	[more]
P26216	3.6e-38	30.11	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1	[more]
P35338	4.7e-38	30.11	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1	[more]

BLAST of Lsi03G008630 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EHY1	6.9e-109	55.38	polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434520 PE=3 SV=1	[more]
A0A6J1EJA0	5.8e-108	54.87	polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434653 PE=3 SV=1	[more]
A0A6J1EMB1	4.2e-106	54.10	polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434652 PE=3 SV=1	[more]
A0A6J1JGG6	1.9e-103	52.82	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486679 PE=3 SV=1	[more]
A0A6J1JPZ1	9.6e-103	52.82	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486677 PE=3 SV=1	[more]

BLAST of Lsi03G008630 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888748.1	1.3e-114	60.27	polygalacturonase-like, partial [Benincasa hispida]	[more]
XP_022927702.1	1.4e-108	55.38	polygalacturonase-like [Cucurbita moschata]	[more]
XP_023531792.1	5.4e-108	54.62	polygalacturonase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022927889.1	1.2e-107	54.87	polygalacturonase-like [Cucurbita moschata]	[more]
XP_022927888.1	8.6e-106	54.10	polygalacturonase-like [Cucurbita moschata]	[more]

BLAST of Lsi03G008630 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G59850.1	2.0e-76	41.78	Pectin lyase-like superfamily protein	[more]
AT2G43870.1	3.4e-76	42.37	Pectin lyase-like superfamily protein	[more]
AT2G43890.1	4.8e-70	38.50	Pectin lyase-like superfamily protein	[more]
AT1G05650.1	2.8e-62	35.49	Pectin lyase-like superfamily protein	[more]
AT2G43880.1	1.8e-61	34.64	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 225..289 e-value: 5.2E-11 score: 42.2 coord: 151..220 e-value: 1.7E-16 score: 60.2 coord: 54..139 e-value: 8.9E-9 score: 34.8
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 181..194
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 155..223 e-value: 2.2E-17 score: 64.8 coord: 224..292 e-value: 8.1E-12 score: 46.6
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 24..154 e-value: 1.3E-25 score: 92.2
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 147..220
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 225..301
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 7..147
None	No IPR available	PANTHER	PTHR31375:SF219	PUTATIVE-RELATED	coord: 225..301
None	No IPR available	PANTHER	PTHR31375:SF219	PUTATIVE-RELATED	coord: 147..220
None	No IPR available	PANTHER	PTHR31375:SF219	PUTATIVE-RELATED	coord: 7..147
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 16..302

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G008630.1	Lsi03G008630.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity