Lsi03G007930 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G007930
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionLaccase
Locationchr03: 11619710 .. 11622438 (+)
RNA-Seq ExpressionLsi03G007930
SyntenyLsi03G007930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCAAGGAAAAACTTATCTTCTCCGAATTGTCAACGCGGTTATGGACGAAGATCTCTTCTTCGGAATCGCAAAACACGAAATGACATTAGTCGGAAAAGACGGGCTCTACACTAAACAAATCAAAACAAATTACCTAATGATCACACCAGGCCAATCCATGGACATATTGATCACTGCAAATCATTCCCCAGGAATCTATATTATGGCTACAAAATCCTACTCAAGTGCTTTTGGTGCTGGCTTTGATAACACCACAGCCACAGCCATTCTAAAGTACTCAACATCACCAAATCCCCAACTCAACCATTTCTTCCCACACTTACCTCCCTACGATAGAACCGAAGCAGCAACGAATTTCACCAAACGTCTACGAAGCTTGACTACTGTCGACGTTCCTTTAAACGTCGACACTCGTTTGTTTTTCGCGTTATCCGTCAATCTAATAGATTGCTCTAATGATCATAAGCCTTGTGCTGGGCCATTTGGGAAGAGATTTGCTGCAAGTATCAACAATGTGAGCTTTGTGACACCATCAGTTTCTTTGCTTGAAGCTTTCTACAACAACGTCGGTGGCGTGTTTACGACGGATTTCCTGGAAAACCCTCCGAGAAAATTCAACTACACCGGCGAGGTTCTACCCGAAAATTTGTTGGCTACTTCTTTTGGGACTAGGGTTATGGTTTTGGAGTATAATGCTAGTGTGGAGGTGATCTTGCAGGGTACTAATATTCTTGCAAGTGATAATCATCCCGTCCATTTGCATGGCTATAGCTTTTACGTTGTAGGATGGGGATTTGGGAATTTCGACCCTGAAACCGACCCGAAACGGTAAAATCTCATCGACCCACCGGAGGAGACGACGGTTGGAGTTCCTAAGAATGGCTGGGTTGCTATCAGATTCAAGGCAAATAATCCAGGTTTGTTTTTCAAGTACAATAATTCTGATGCAAACTTTAACTTTGATTTAATTTAAATTCTATTTACACTTGTTTTGAAATTTGGAAGTTAGTGTTTCCCTATGGTTTCTCCATCAATTTTCCGTTTTTTTTAGTGTACATGTTTTGAATAAAAAACTAAGGCTACTAACCATTTGGTTTTTAGTCTTTAGTTTTTGAAAATTAAATCTATTTTCTCTCCATCTTTTACAATGGTATATTTATATGCTTATGTAAAATAGTTGAATTCTTAGCCAAATTCTAAAAGAAAAACTACTACTTTAAAAACTACTTTTCTTTTTTTAGTTTTTAAAAATTGGTTTAGTTTTTGAAAAAATGGGTTAAATTATATAACAAAACAAAAAAAATTTAGAAGTGAGGCTTAATTTTCAAAGACTAAAAATCAAAAACCAAAAATCAAGGCCTTGTTTGGTATCCATTTCCTTTTTCCTTTTTGGTTTTTGAAAAGTAAGTCTATTTTCTCTCAATTTCTTATAGTGATTTGCATCTTTCTTAAACGGAAGAGTTGATTTTTTAGCCAAATTCTAAAAACAAAAACAAGTTTTTTAAAAACTACTTTTTTTTAGTTTTCAAATTTTGGTTTTGTTTTTTAAAACACTAGTAAAAAGTAGATAACAAATCAAAAAATTTATAGGTGGTAGGAGTGTTTATAAGCTTCATTTTCAGAAACAAAAAATAAAAAACCAAATGATTACCAAACAGAGCCCAAATGTTACCAAACGGGGAGTAAGTTTTTTTTTTTGAACATTTAATAAAAATATTAATCTAAACTTACGTTTAAGAAAATATGTTTAAAAGATTAAATGTATTATAATCTTGGTTAAATTATAAATTTGGTCCCATAATTAAGAGAAAGTTAGAATTTGATCCCTATAATTTTAAAACTTAAAATTTAGTCGTTATGATTTGATAAAACCTCATAAATAATAGTCTATATGGTTTGTTAAACCCCTCCGAAATAGTATCTACCATAGGGACTAAATTATACTTATAAACCATATGAACTAAATTCTAACTAATTTCTTTAAAACATAAGGACCAAATTTATAATTTAACAATCTATAAATCTATTGTTACATCGATCAGGAGGTCAAAAGTTTGAATCTTCTACTATGCATATTGCTGTACTAATAAAAACAAACTACTACATATCTAACTTATTAAAGGAAAGAATAATGTATTGAATTAGATAATTAAATTGAAAGAATAATGCGTATTGAATTAGATATATAATTAAATTTATATTGATCCATATATATGTCAATGATTATTCATTATTAACTAAGCTCTTATTTAAAAGAGCATTTGCAATGGATAGCACTTTTAAGGTTAATAATTAAGTGTATAGCAATATTTTTAAAAAATTATAATTATATCAAAATCTATCGGCGGTAGACTCCTGTCTATCACTGATAGACTCTGAGAGTTAAATCTAAATTTTGCTATATCTTCATAAAAAAATTACATTATATTATATCTACTAATATTTTGGATATGATTACTATGTTTGCAACTGTCTCTATTTAAAACAGACTTTCTAAGCGTTTTTTTAACATTATTAATCATTCTAAATATGTCCTATATATGTTTGAAAAAACTAATTAAATCTCTCTTATTTGATACTTTTATAGGTATGTGGTTGATGCATTGTCATATAGAGCGTCACCAAGTATGGGGCATGGGCATGGTGTTCTTAGTGAAAAATGGACTTGCTTCACACCAACAAATTCTTAACCCTCCACATGATTTGCCTTCATGCTATGATTAG

mRNA sequence

ATGGAGCAAGGAAAAACTTATCTTCTCCGAATTGTCAACGCGGTTATGGACGAAGATCTCTTCTTCGGAATCGCAAAACACGAAATGACATTAGTCGGAAAAGACGGGCTCTACACTAAACAAATCAAAACAAATTACCTAATGATCACACCAGGCCAATCCATGGACATATTGATCACTGCAAATCATTCCCCAGGAATCTATATTATGGCTACAAAATCCTACTCAAGTGCTTTTGGTGCTGGCTTTGATAACACCACAGCCACAGCCATTCTAAAGTACTCAACATCACCAAATCCCCAACTCAACCATTTCTTCCCACACTTACCTCCCTACGATAGAACCGAAGCAGCAACGAATTTCACCAAACGTCTACGAAGCTTGACTACTGTCGACGTTCCTTTAAACGTCGACACTCGTTTGTTTTTCGCGTTATCCGTCAATCTAATAGATTGCTCTAATGATCATAAGCCTTGTGCTGGGCCATTTGGGAAGAGATTTGCTGCAAGTATCAACAATGTGAGCTTTGTGACACCATCAGTTTCTTTGCTTGAAGCTTTCTACAACAACGTCGGTGGCGTGTTTACGACGGATTTCCTGGAAAACCCTCCGAGAAAATTCAACTACACCGGCGAGGTTCTACCCGAAAATTTGTTGGCTACTTCTTTTGGGACTAGGGTTATGGTTTTGGAGTATAATGCTAGTGTGGAGGTGATCTTGCAGGGTACTAATATTCTTGCAAGTGATAATCATCCCGTCCATTTGCATGGCTATAGCTTTTACGTTGTAGGATGGGGATTTGGGAATTTCGACCCTGAAACCGACCCGAAACGATTCAAGGCAAATAATCCAGGTATGTGGTTGATGCATTGTCATATAGAGCGTCACCAAGTATGGGGCATGGGCATGGTGTTCTTAGTGAAAAATGGACTTGCTTCACACCAACAAATTCTTAACCCTCCACATGATTTGCCTTCATGCTATGATTAG

Coding sequence (CDS)

ATGGAGCAAGGAAAAACTTATCTTCTCCGAATTGTCAACGCGGTTATGGACGAAGATCTCTTCTTCGGAATCGCAAAACACGAAATGACATTAGTCGGAAAAGACGGGCTCTACACTAAACAAATCAAAACAAATTACCTAATGATCACACCAGGCCAATCCATGGACATATTGATCACTGCAAATCATTCCCCAGGAATCTATATTATGGCTACAAAATCCTACTCAAGTGCTTTTGGTGCTGGCTTTGATAACACCACAGCCACAGCCATTCTAAAGTACTCAACATCACCAAATCCCCAACTCAACCATTTCTTCCCACACTTACCTCCCTACGATAGAACCGAAGCAGCAACGAATTTCACCAAACGTCTACGAAGCTTGACTACTGTCGACGTTCCTTTAAACGTCGACACTCGTTTGTTTTTCGCGTTATCCGTCAATCTAATAGATTGCTCTAATGATCATAAGCCTTGTGCTGGGCCATTTGGGAAGAGATTTGCTGCAAGTATCAACAATGTGAGCTTTGTGACACCATCAGTTTCTTTGCTTGAAGCTTTCTACAACAACGTCGGTGGCGTGTTTACGACGGATTTCCTGGAAAACCCTCCGAGAAAATTCAACTACACCGGCGAGGTTCTACCCGAAAATTTGTTGGCTACTTCTTTTGGGACTAGGGTTATGGTTTTGGAGTATAATGCTAGTGTGGAGGTGATCTTGCAGGGTACTAATATTCTTGCAAGTGATAATCATCCCGTCCATTTGCATGGCTATAGCTTTTACGTTGTAGGATGGGGATTTGGGAATTTCGACCCTGAAACCGACCCGAAACGATTCAAGGCAAATAATCCAGGTATGTGGTTGATGCATTGTCATATAGAGCGTCACCAAGTATGGGGCATGGGCATGGTGTTCTTAGTGAAAAATGGACTTGCTTCACACCAACAAATTCTTAACCCTCCACATGATTTGCCTTCATGCTATGATTAG

Protein sequence

MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVTPSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPKRFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSCYD
Homology
BLAST of Lsi03G007930 vs. ExPASy Swiss-Prot
Match: Q9FY79 (Laccase-14 OS=Arabidopsis thaliana OX=3702 GN=LAC14 PE=2 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 1.4e-115
Identity = 198/348 (56.90%), Postives = 251/348 (72.13%), Query Frame = 0

Query: 3   QGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITAN 62
           +G+ YLLRI+NAVMDE+LFF IA H +T+V KDG Y K  K++YLMITPGQSMD+L+ AN
Sbjct: 226 RGRRYLLRIINAVMDEELFFAIANHTLTVVAKDGFYLKHFKSDYLMITPGQSMDVLLHAN 285

Query: 63  HSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATNFT 122
             P  Y +A ++YSSAFGAGFD TT TAIL+Y      ++    P+LPPY+RTEA+T FT
Sbjct: 286 QRPNHYFVAARAYSSAFGAGFDKTTTTAILQYKGDTLNRIKPILPYLPPYNRTEASTRFT 345

Query: 123 KRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVTPSVS 182
            + RS   V+VP+ ++TRL +A+SVNL++CS+D +PC GPFGKRF++SINN+SFV PSV 
Sbjct: 346 NQFRSQRPVNVPVKINTRLLYAISVNLMNCSDD-RPCTGPFGKRFSSSINNISFVNPSVD 405

Query: 183 LLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEVILQG 242
           +L A+Y ++GGVF  DF  NPP KFNYTGE LP     T FGT+V+VL+YN+SVE+ILQG
Sbjct: 406 ILRAYYRHIGGVFQEDFPRNPPTKFNYTGENLP---FPTRFGTKVVVLDYNSSVELILQG 465

Query: 243 TNILASDNHPVHLHGYSFYVVGWGFGNFDPETDP-----------------------KRF 302
           T + AS+ HP+HLHGY+FYVVG GFGNFD   DP                        RF
Sbjct: 466 TTVWASNIHPIHLHGYNFYVVGSGFGNFDRRKDPLRYNLVDPPEETTVGVPRNGWTAVRF 525

Query: 303 KANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
            ANNPG+WL+HCHIERH  WGM  VF+VK+G     +++ PP DLPSC
Sbjct: 526 VANNPGVWLLHCHIERHATWGMNTVFIVKDGPTKSSRMVKPPPDLPSC 569

BLAST of Lsi03G007930 vs. ExPASy Swiss-Prot
Match: Q56YT0 (Laccase-3 OS=Arabidopsis thaliana OX=3702 GN=LAC3 PE=2 SV=2)

HSP 1 Score: 323.2 bits (827), Expect = 3.5e-87
Identity = 167/355 (47.04%), Postives = 220/355 (61.97%), Query Frame = 0

Query: 4   GKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANH 63
           G+T  LR++NA M+++LFF +A H+ T+V  D  YTK   TN +MI PGQ+ ++L+TAN 
Sbjct: 218 GETVQLRVINAGMNQELFFSVANHQFTVVETDSAYTKPFTTNVIMIGPGQTTNVLLTANQ 277

Query: 64  SPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSP------NPQLNHFFPHLPPYDRTEA 123
            PG Y MA ++Y+SA  A FDNTT TAIL+Y  +P        Q+   FP LP ++ T  
Sbjct: 278 RPGRYYMAARAYNSA-NAPFDNTTTTAILQYVNAPTRRGRGRGQIAPVFPVLPGFNDTAT 337

Query: 124 ATNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKP-CAGPFGKRFAASINNVSF 183
           AT FT RLR      VP  VD  LFF + + LI+C+N + P C GP G RFAAS+NN+SF
Sbjct: 338 ATAFTNRLRYWKRAPVPQQVDENLFFTVGLGLINCANPNSPRCQGPNGTRFAASMNNMSF 397

Query: 184 VTP-SVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNAS 243
           V P S S+++A+Y    G+FTTDF   PP +F+YTG V    L     GT+   L+Y ++
Sbjct: 398 VLPRSNSVMQAYYQGTPGIFTTDFPPVPPVQFDYTGNV-SRGLWQPIKGTKAYKLKYKSN 457

Query: 244 VEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPKRFK--------------- 303
           V+++LQ T+I+  +NHP+HLHGY FYVVG GFGNF+P TDP RF                
Sbjct: 458 VQIVLQDTSIVTPENHPMHLHGYQFYVVGSGFGNFNPRTDPARFNLFDPPERNTIGTPPG 517

Query: 304 --------ANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
                   A+NPG W MHCHI+ H  WG+ MVFLV+NG    Q +  PP DLP C
Sbjct: 518 GWVAIRFVADNPGAWFMHCHIDSHLGWGLAMVFLVENGRGQLQSVQAPPLDLPRC 570

BLAST of Lsi03G007930 vs. ExPASy Swiss-Prot
Match: Q9FLB5 (Laccase-12 OS=Arabidopsis thaliana OX=3702 GN=LAC12 PE=2 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.1e-83
Identity = 156/353 (44.19%), Postives = 213/353 (60.34%), Query Frame = 0

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           +  G+T LLR++NA +++ LFF +A H++T+VG D  Y K   T  LM+ PGQ+ D+L+T
Sbjct: 214 INSGETSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLMLGPGQTTDVLLT 273

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATN 120
           A+  P  Y +A ++Y SA  A FDNTT TAIL+Y  +         P LP ++ T   T+
Sbjct: 274 ADQPPKRYYIAARAYQSAQNAPFDNTTTTAILQYKKTTTTS-KPIMPVLPAFNDTNTVTS 333

Query: 121 FTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSN--DHKPCAGPFGKRFAASINNVSFVT 180
           F+++ +SL  V VP  +D  LFF + + L +C        C G  G RF AS+NNVSFV 
Sbjct: 334 FSRKFKSLRNVVVPKTIDDNLFFTIGLGLDNCPKKFPKSRCQGLNGTRFTASMNNVSFVL 393

Query: 181 PS-VSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVE 240
           PS  SLL+A  N + GVFTTDF   PP KF+YTG  +   L     GT++  L+Y + V+
Sbjct: 394 PSNFSLLQAHSNGIPGVFTTDFPSKPPVKFDYTGNNISRALFQPVKGTKLYKLKYGSRVQ 453

Query: 241 VILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK-------------------- 300
           V+LQ TNI+ S+NHP+HLHGY FY+VG GFGNF+P+ D                      
Sbjct: 454 VVLQDTNIVTSENHPIHLHGYDFYIVGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGW 513

Query: 301 ---RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
              RF A+NPG+WLMHCH++ H  WG+ M FLV NG+   + +  PPHDLP C
Sbjct: 514 AVIRFVADNPGVWLMHCHLDVHIKWGLAMAFLVDNGVGELETLEAPPHDLPIC 565

BLAST of Lsi03G007930 vs. ExPASy Swiss-Prot
Match: Q2RBK2 (Putative laccase-17 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC17 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.5e-82
Identity = 159/351 (45.30%), Postives = 211/351 (60.11%), Query Frame = 0

Query: 4   GKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANH 63
           G+T LLR +NA ++ +LF  +A H MT+V  D  YTK   T+ L++ PGQ+ D+L+T + 
Sbjct: 217 GETNLLRFINAALNTELFVSLAGHNMTVVAADASYTKPYTTSLLLLAPGQTTDVLVTFDQ 276

Query: 64  SPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHF-FPHLPPYDRTEAATNFT 123
            PG Y +A ++Y+SA G  FDNTT TAI  Y  + N        P LP Y+ T AAT FT
Sbjct: 277 PPGRYYLAARAYASAQGVPFDNTTTTAIFDYGAANNASSAAIAMPTLPAYNDTTAATAFT 336

Query: 124 KRLRSLTTVDVPLNVDTRLFFALSVNLIDCSN-DHKPCAGPFGKRFAASINNVSFVTP-S 183
             LR L   ++P  VD  LFF + V L +C+N   + C GP G RFAASINNVSFV P S
Sbjct: 337 TNLRGLRKAELPSRVDESLFFTVGVGLFNCTNATAQQCGGPNGTRFAASINNVSFVLPSS 396

Query: 184 VSLLEAFYNNV-GGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEVI 243
            S+L+A ++   GGVFT DF  NPP +F+YT + +   L     GT+V  L+Y ++V+V+
Sbjct: 397 TSILQAHHHGAPGGVFTADFPANPPVQFDYTAQNVSRALWQPVAGTKVYKLKYGSAVQVV 456

Query: 244 LQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK---------------------- 303
           LQGTNI A +NHP+HLHGY FY++  G GNFD   D                        
Sbjct: 457 LQGTNIFAGENHPIHLHGYDFYILAEGLGNFDAGADTGKFNVEDPPMRNTVGVPVNGWAV 516

Query: 304 -RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
            RF A+NPG+WLMHCH++ H  WG+ M FLV +G+   Q +  PP DLP C
Sbjct: 517 IRFVADNPGVWLMHCHLDVHITWGLAMAFLVDDGVGELQSLEAPPPDLPLC 567

BLAST of Lsi03G007930 vs. ExPASy Swiss-Prot
Match: Q9LYQ2 (Laccase-13 OS=Arabidopsis thaliana OX=3702 GN=LAC13 PE=2 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 7.5e-82
Identity = 159/356 (44.66%), Postives = 215/356 (60.39%), Query Frame = 0

Query: 4   GKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANH 63
           G+  LLR++N+ ++++LFFG+A H++T+V  D  YTK   TN +M+ PGQ+ D+L+TA+ 
Sbjct: 216 GEIVLLRVINSALNQELFFGVANHKLTVVAADASYTKPFSTNVIMLGPGQTTDVLLTADQ 275

Query: 64  SPGIYIMATKSYSSAFGAGFDNTTATAILKY------STSPNPQLNHFFPHLPPYDRTEA 123
            P  Y MA  +Y+SA  A FDNTT TAILKY      +     Q       LP ++ T  
Sbjct: 276 PPAHYYMAAHAYNSA-NAAFDNTTTTAILKYKDASCVTLQAKSQARAIPAQLPGFNDTAT 335

Query: 124 ATNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDH-KPCAGPFGKRFAASINNVSF 183
           A  FT +++S + V VPL +D  LFF + + L +C   + + C GP G RF ASINNVSF
Sbjct: 336 AAAFTAQMKSPSKVKVPLEIDENLFFTVGLGLFNCPTPNTQRCQGPNGTRFTASINNVSF 395

Query: 184 VTPSV-SLLEAFYNNV-GGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNA 243
           V P   S+++A+Y     GVFTTDF   PP  F+YTG V    L   + GT+   L++N+
Sbjct: 396 VFPKQNSIMQAYYQGTPTGVFTTDFPPTPPVTFDYTGNV-SRGLWQPTRGTKAYKLKFNS 455

Query: 244 SVEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK----------------- 303
            V++ILQ T+I+ ++NHP+HLHGY FYVVG G GNF+P TD                   
Sbjct: 456 QVQIILQDTSIVTTENHPMHLHGYEFYVVGTGVGNFNPNTDTSSFNLIDPPRRNTIGTPP 515

Query: 304 ------RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
                 RF ANNPG WLMHCHI+ H  WG+ MVFLV+NG    Q + +PP DLP C
Sbjct: 516 GGWVAIRFVANNPGAWLMHCHIDSHIFWGLAMVFLVENGEGHLQSVQSPPLDLPQC 569

BLAST of Lsi03G007930 vs. ExPASy TrEMBL
Match: A0A1S3C199 (Laccase OS=Cucumis melo OX=3656 GN=LOC103495685 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.4e-165
Identity = 289/354 (81.64%), Postives = 311/354 (87.85%), Query Frame = 0

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           ME+GKTYLLRIV+AVMDEDLFFGIAKH+MTLVGKDG+Y KQIKT+Y+MITPGQSMDILIT
Sbjct: 210 MEKGKTYLLRIVSAVMDEDLFFGIAKHKMTLVGKDGIYMKQIKTDYIMITPGQSMDILIT 269

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYS--TSPNPQLNHFFPHLPPYDRTEAA 120
           AN SPG+YIMAT+SYSSAFGAGFDNTTATAILKYS  TSPNP  N FFPHLPPYDRTEAA
Sbjct: 270 ANQSPGMYIMATRSYSSAFGAGFDNTTATAILKYSTITSPNPP-NPFFPHLPPYDRTEAA 329

Query: 121 TNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVT 180
           T+FTKRLRSL TVDV LNVDTRLFF LSVNL+DCSN+ KPCAGPFGKRFAASINNVSFVT
Sbjct: 330 TDFTKRLRSLRTVDVSLNVDTRLFFTLSVNLMDCSNEDKPCAGPFGKRFAASINNVSFVT 389

Query: 181 PSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEV 240
           PSVSLLEA++N VGGVFTTDF ENPPRKFNYTGE LP+ LL TSFGT+VMVLEYNASVE+
Sbjct: 390 PSVSLLEAYHNRVGGVFTTDFPENPPRKFNYTGENLPKKLLPTSFGTKVMVLEYNASVEL 449

Query: 241 ILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK--------------------- 300
           ILQGTN+LASDNHPVHLHGYSFYVVGWGFGNFDP+ DPK                     
Sbjct: 450 ILQGTNVLASDNHPVHLHGYSFYVVGWGFGNFDPKNDPKRYNLVDPPEETTVGVPKNGWV 509

Query: 301 --RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSCYD 330
             RFKA+NPGMWLMHCHIERHQ WGM MVFLVK+GLA HQQIL+PPHDLPSCY+
Sbjct: 510 AIRFKADNPGMWLMHCHIERHQAWGMNMVFLVKDGLAPHQQILHPPHDLPSCYN 562

BLAST of Lsi03G007930 vs. ExPASy TrEMBL
Match: A0A6J1DLZ8 (Laccase OS=Momordica charantia OX=3673 GN=LOC111021676 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.2e-154
Identity = 273/356 (76.69%), Postives = 303/356 (85.11%), Query Frame = 0

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLV KDG+YTKQIKT+Y+MITPGQSMD+L+T
Sbjct: 214 MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVAKDGIYTKQIKTHYIMITPGQSMDLLVT 273

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATN 120
           A+ SPG+Y MA +SYSSA GAGFDNTTATAIL YS  PN  LNHFFP+LPPYD T+AAT+
Sbjct: 274 ADQSPGLYFMAARSYSSALGAGFDNTTATAILNYS-KPN-HLNHFFPNLPPYDATKAATD 333

Query: 121 FTKRLRSLT----TVDVPLNVDTRLFFALSVNLIDC-SNDHKPCAGPFGKRFAASINNVS 180
           FTKRLRSLT      DVPLN+DTRLFF LSVNL+ C ++DH+ CAGPFGKRFAASINNVS
Sbjct: 334 FTKRLRSLTIDDRRADVPLNIDTRLFFTLSVNLMSCNASDHRACAGPFGKRFAASINNVS 393

Query: 181 FVTPSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNAS 240
           FVTPSV++LEA+YN+V GVFTT+F   PPRKF+YTG+ L ENLLATSFGTRV+VLEYNAS
Sbjct: 394 FVTPSVAMLEAYYNDVHGVFTTNFPRKPPRKFDYTGKNLSENLLATSFGTRVIVLEYNAS 453

Query: 241 VEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK------------------ 300
           VE+ILQGTN+LASDNHPVHLHGYSFYVVGWGFGNF+P+TDPK                  
Sbjct: 454 VELILQGTNVLASDNHPVHLHGYSFYVVGWGFGNFNPKTDPKRYNLVNPXEETTVGVPKN 513

Query: 301 -----RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSCY 329
                RFKANNPGMWLMHCHIERHQVWGM MV LVKNGLA HQQIL+PPHDLPSC+
Sbjct: 514 GWVAIRFKANNPGMWLMHCHIERHQVWGMSMVLLVKNGLAPHQQILHPPHDLPSCF 567

BLAST of Lsi03G007930 vs. ExPASy TrEMBL
Match: A0A6J1EPR2 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111434523 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.4e-147
Identity = 258/355 (72.68%), Postives = 290/355 (81.69%), Query Frame = 0

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           +E+GKTYLLR++NAVMDEDLFF IAKHEMTLVGKDG+Y KQIKTNY+MITPGQSMD+LIT
Sbjct: 221 VERGKTYLLRMINAVMDEDLFFAIAKHEMTLVGKDGIYMKQIKTNYIMITPGQSMDLLIT 280

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATN 120
           AN +PG Y MAT+SYSSAFGAGFDNTTATAILKYST  +    HFFP LPPYDRTEA+T+
Sbjct: 281 ANQAPGTYFMATRSYSSAFGAGFDNTTATAILKYSTKSSIPSFHFFPTLPPYDRTEASTD 340

Query: 121 FTKRLRSLT----TVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSF 180
           FTK+ RSLT      DVPL +DTRL F LSVNL++CS   KPCAG FGKRFAAS+NNVSF
Sbjct: 341 FTKQFRSLTINGRRADVPLKIDTRLLFTLSVNLLNCST-KKPCAGAFGKRFAASVNNVSF 400

Query: 181 VTPSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASV 240
           V PS+SLL+A+Y  V GVFT DF +NP RKFNYT EV+PE L++TSFGTRVMVLEYNASV
Sbjct: 401 VAPSLSLLQAYYYKVPGVFTRDFPKNPTRKFNYTAEVIPEALMSTSFGTRVMVLEYNASV 460

Query: 241 EVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK------------------- 300
           E++LQGTN++ASDNHPVHLHGYSFYVVGWGFGNFDP+TD K                   
Sbjct: 461 ELVLQGTNVVASDNHPVHLHGYSFYVVGWGFGNFDPKTDTKRYNLVDPPEETTVGVPKNG 520

Query: 301 ----RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSCY 329
               RFKANNPGMWLMHCH+ERHQVWGM MVFLVKNG A  Q+I+ PPHDLP CY
Sbjct: 521 WVAIRFKANNPGMWLMHCHLERHQVWGMSMVFLVKNGHARDQKIVRPPHDLPKCY 574

BLAST of Lsi03G007930 vs. ExPASy TrEMBL
Match: A0A6J1JN53 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111485978 PE=3 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 2.1e-143
Identity = 254/355 (71.55%), Postives = 285/355 (80.28%), Query Frame = 0

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           +E GKTYLLR++NAVMDEDLFF IAKHEMTLVGKDG+Y KQIKT+YLMITPGQSMD+LIT
Sbjct: 221 VEHGKTYLLRMINAVMDEDLFFAIAKHEMTLVGKDGIYMKQIKTDYLMITPGQSMDLLIT 280

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATN 120
           AN +PG Y MAT+SYSSAFGAGFDN+TATAILKYST  +    HFFP LPPYDRTEA T+
Sbjct: 281 ANQAPGTYFMATRSYSSAFGAGFDNSTATAILKYSTKSSIPTFHFFPTLPPYDRTEATTD 340

Query: 121 FTKRLRSLT----TVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSF 180
           FTK+ RSLT      DVP+ +DTRL F LSVNL++CS   KPCAG FGKRFAAS+NNVSF
Sbjct: 341 FTKQFRSLTINGRRADVPMKIDTRLLFTLSVNLLNCST-KKPCAGAFGKRFAASVNNVSF 400

Query: 181 VTPSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASV 240
           V PS+SLLEA+Y  V GVFT DF +NP +KFNYT EV+PE  L+TSFGTRVMVLEYNASV
Sbjct: 401 VAPSLSLLEAYYYKVAGVFTRDFPKNPTKKFNYTAEVIPEAFLSTSFGTRVMVLEYNASV 460

Query: 241 EVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK------------------- 300
           E++LQGTN++ASDNHPVHLHGYSFYVVG GFGNFD +TD K                   
Sbjct: 461 ELVLQGTNVVASDNHPVHLHGYSFYVVGGGFGNFDLKTDMKQYNLVDPPEETTVGVPKNG 520

Query: 301 ----RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSCY 329
               RFKANNPGMWLMHCH+ERHQVWGM MVFLVKNG A  Q+I+ PPHDLP CY
Sbjct: 521 WVAIRFKANNPGMWLMHCHLERHQVWGMSMVFLVKNGHARDQKIVRPPHDLPKCY 574

BLAST of Lsi03G007930 vs. ExPASy TrEMBL
Match: A0A0A0K3Y1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G058630 PE=4 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.1e-139
Identity = 253/313 (80.83%), Postives = 273/313 (87.22%), Query Frame = 0

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           MEQGKTYLLRIV+AVMDE+LFFGIAKH+MTLVGKDG+YTKQ KT+Y+MITPGQSMDILIT
Sbjct: 1   MEQGKTYLLRIVSAVMDEELFFGIAKHKMTLVGKDGIYTKQFKTDYIMITPGQSMDILIT 60

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYS--TSPNPQLNHFFPHLPPYDRTEAA 120
           AN SPG+YIMAT+SYSSAFGAGFDNTTATAILKYS  TSPNP  N FFPHLPPYDRTEAA
Sbjct: 61  ANQSPGMYIMATRSYSSAFGAGFDNTTATAILKYSTITSPNPP-NPFFPHLPPYDRTEAA 120

Query: 121 TNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVT 180
           T+FTKRLRSLTTVDV L VDTRLFF LSVNL+DCSN+ KPCAGPFGKRFAASINNVSFVT
Sbjct: 121 TDFTKRLRSLTTVDVSLEVDTRLFFTLSVNLMDCSNEDKPCAGPFGKRFAASINNVSFVT 180

Query: 181 PSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEV 240
           PSVSLLEA++N +GGVFTTDF  NPPRKFNYTGE LPE LL TSFGT+VMVLEYNASVE+
Sbjct: 181 PSVSLLEAYHNKIGGVFTTDFPGNPPRKFNYTGENLPEKLLPTSFGTKVMVLEYNASVEL 240

Query: 241 ILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK--------------------- 289
           ILQGTN+LASDNHPVHLHGYSFYVVGWGFGNFDP+TDPK                     
Sbjct: 241 ILQGTNVLASDNHPVHLHGYSFYVVGWGFGNFDPKTDPKRYNLVDPPEETTVGVPKNGWV 300

BLAST of Lsi03G007930 vs. NCBI nr
Match: XP_038887094.1 (laccase-14 [Benincasa hispida])

HSP 1 Score: 598.6 bits (1542), Expect = 3.3e-167
Identity = 294/353 (83.29%), Postives = 312/353 (88.39%), Query Frame = 0

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDG+YTKQIKT+Y+MITPGQSMDILIT
Sbjct: 221 MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGIYTKQIKTDYIMITPGQSMDILIT 280

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTS-PNPQLNHFFPHLPPYDRTEAAT 120
           AN SPGIYIMAT+SYSSAFGAGFDN+TA AILKYST+ P P+LNHFFPHLPPYDRTEAAT
Sbjct: 281 ANQSPGIYIMATRSYSSAFGAGFDNSTAIAILKYSTTPPTPKLNHFFPHLPPYDRTEAAT 340

Query: 121 NFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVTP 180
           +FTKRLRSLTTVDVPLNVDTRLFF LSVNL++CSN+ KPCAGPFGKRFAASINNVSFVTP
Sbjct: 341 DFTKRLRSLTTVDVPLNVDTRLFFVLSVNLMNCSNNDKPCAGPFGKRFAASINNVSFVTP 400

Query: 181 SVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEVI 240
           S SLLEA+YNNV GVFTTDF ENPPRKFNYTGE LP   LATSFGTRVMVLEYNASVEVI
Sbjct: 401 SASLLEAYYNNVSGVFTTDFPENPPRKFNYTGENLP---LATSFGTRVMVLEYNASVEVI 460

Query: 241 LQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK---------------------- 300
           LQGTN+LASDNHPVHLHGYSF+VVGWG GNF+P+TDPK                      
Sbjct: 461 LQGTNVLASDNHPVHLHGYSFHVVGWGLGNFNPKTDPKHYNLVDPPEETTVGVPNNGWVA 520

Query: 301 -RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSCYD 330
            RFKANNPGMWLMHCHIERHQVWGM MVFLVKNG A  QQIL+PPHDLPSCY+
Sbjct: 521 IRFKANNPGMWLMHCHIERHQVWGMSMVFLVKNGFALDQQILHPPHDLPSCYN 570

BLAST of Lsi03G007930 vs. NCBI nr
Match: XP_008455534.1 (PREDICTED: laccase-14 [Cucumis melo])

HSP 1 Score: 589.7 bits (1519), Expect = 1.5e-164
Identity = 289/354 (81.64%), Postives = 311/354 (87.85%), Query Frame = 0

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           ME+GKTYLLRIV+AVMDEDLFFGIAKH+MTLVGKDG+Y KQIKT+Y+MITPGQSMDILIT
Sbjct: 210 MEKGKTYLLRIVSAVMDEDLFFGIAKHKMTLVGKDGIYMKQIKTDYIMITPGQSMDILIT 269

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYS--TSPNPQLNHFFPHLPPYDRTEAA 120
           AN SPG+YIMAT+SYSSAFGAGFDNTTATAILKYS  TSPNP  N FFPHLPPYDRTEAA
Sbjct: 270 ANQSPGMYIMATRSYSSAFGAGFDNTTATAILKYSTITSPNPP-NPFFPHLPPYDRTEAA 329

Query: 121 TNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVT 180
           T+FTKRLRSL TVDV LNVDTRLFF LSVNL+DCSN+ KPCAGPFGKRFAASINNVSFVT
Sbjct: 330 TDFTKRLRSLRTVDVSLNVDTRLFFTLSVNLMDCSNEDKPCAGPFGKRFAASINNVSFVT 389

Query: 181 PSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEV 240
           PSVSLLEA++N VGGVFTTDF ENPPRKFNYTGE LP+ LL TSFGT+VMVLEYNASVE+
Sbjct: 390 PSVSLLEAYHNRVGGVFTTDFPENPPRKFNYTGENLPKKLLPTSFGTKVMVLEYNASVEL 449

Query: 241 ILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK--------------------- 300
           ILQGTN+LASDNHPVHLHGYSFYVVGWGFGNFDP+ DPK                     
Sbjct: 450 ILQGTNVLASDNHPVHLHGYSFYVVGWGFGNFDPKNDPKRYNLVDPPEETTVGVPKNGWV 509

Query: 301 --RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSCYD 330
             RFKA+NPGMWLMHCHIERHQ WGM MVFLVK+GLA HQQIL+PPHDLPSCY+
Sbjct: 510 AIRFKADNPGMWLMHCHIERHQAWGMNMVFLVKDGLAPHQQILHPPHDLPSCYN 562

BLAST of Lsi03G007930 vs. NCBI nr
Match: XP_004137046.1 (laccase-14 [Cucumis sativus])

HSP 1 Score: 587.4 bits (1513), Expect = 7.6e-164
Identity = 289/352 (82.10%), Postives = 309/352 (87.78%), Query Frame = 0

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           MEQGKTYLLRIV+AVMDE+LFFGIAKH+MTLVGKDG+YTKQ KT+Y+MITPGQSMDILIT
Sbjct: 221 MEQGKTYLLRIVSAVMDEELFFGIAKHKMTLVGKDGIYTKQFKTDYIMITPGQSMDILIT 280

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYS--TSPNPQLNHFFPHLPPYDRTEAA 120
           AN SPG+YIMAT+SYSSAFGAGFDNTTATAILKYS  TSPNP  N FFPHLPPYDRTEAA
Sbjct: 281 ANQSPGMYIMATRSYSSAFGAGFDNTTATAILKYSTITSPNPP-NPFFPHLPPYDRTEAA 340

Query: 121 TNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVT 180
           T+FTKRLRSLTTVDV L VDTRLFF LSVNL+DCSN+ KPCAGPFGKRFAASINNVSFVT
Sbjct: 341 TDFTKRLRSLTTVDVSLEVDTRLFFTLSVNLMDCSNEDKPCAGPFGKRFAASINNVSFVT 400

Query: 181 PSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEV 240
           PSVSLLEA++N +GGVFTTDF  NPPRKFNYTGE LPE LL TSFGT+VMVLEYNASVE+
Sbjct: 401 PSVSLLEAYHNKIGGVFTTDFPGNPPRKFNYTGENLPEKLLPTSFGTKVMVLEYNASVEL 460

Query: 241 ILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK--------------------- 300
           ILQGTN+LASDNHPVHLHGYSFYVVGWGFGNFDP+TDPK                     
Sbjct: 461 ILQGTNVLASDNHPVHLHGYSFYVVGWGFGNFDPKTDPKRYNLVDPPEETTVGVPKNGWV 520

Query: 301 --RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
             RFKANNPGMWLMHCHIERHQVWGM MVFLVK+GLA HQQIL+ PHDLPSC
Sbjct: 521 AIRFKANNPGMWLMHCHIERHQVWGMTMVFLVKDGLAPHQQILHSPHDLPSC 571

BLAST of Lsi03G007930 vs. NCBI nr
Match: KGN43742.2 (hypothetical protein Csa_017248 [Cucumis sativus])

HSP 1 Score: 587.4 bits (1513), Expect = 7.6e-164
Identity = 289/352 (82.10%), Postives = 309/352 (87.78%), Query Frame = 0

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           MEQGKTYLLRIV+AVMDE+LFFGIAKH+MTLVGKDG+YTKQ KT+Y+MITPGQSMDILIT
Sbjct: 144 MEQGKTYLLRIVSAVMDEELFFGIAKHKMTLVGKDGIYTKQFKTDYIMITPGQSMDILIT 203

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYS--TSPNPQLNHFFPHLPPYDRTEAA 120
           AN SPG+YIMAT+SYSSAFGAGFDNTTATAILKYS  TSPNP  N FFPHLPPYDRTEAA
Sbjct: 204 ANQSPGMYIMATRSYSSAFGAGFDNTTATAILKYSTITSPNPP-NPFFPHLPPYDRTEAA 263

Query: 121 TNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVT 180
           T+FTKRLRSLTTVDV L VDTRLFF LSVNL+DCSN+ KPCAGPFGKRFAASINNVSFVT
Sbjct: 264 TDFTKRLRSLTTVDVSLEVDTRLFFTLSVNLMDCSNEDKPCAGPFGKRFAASINNVSFVT 323

Query: 181 PSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEV 240
           PSVSLLEA++N +GGVFTTDF  NPPRKFNYTGE LPE LL TSFGT+VMVLEYNASVE+
Sbjct: 324 PSVSLLEAYHNKIGGVFTTDFPGNPPRKFNYTGENLPEKLLPTSFGTKVMVLEYNASVEL 383

Query: 241 ILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK--------------------- 300
           ILQGTN+LASDNHPVHLHGYSFYVVGWGFGNFDP+TDPK                     
Sbjct: 384 ILQGTNVLASDNHPVHLHGYSFYVVGWGFGNFDPKTDPKRYNLVDPPEETTVGVPKNGWV 443

Query: 301 --RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
             RFKANNPGMWLMHCHIERHQVWGM MVFLVK+GLA HQQIL+ PHDLPSC
Sbjct: 444 AIRFKANNPGMWLMHCHIERHQVWGMTMVFLVKDGLAPHQQILHSPHDLPSC 494

BLAST of Lsi03G007930 vs. NCBI nr
Match: XP_022154399.1 (laccase-14 [Momordica charantia])

HSP 1 Score: 555.8 bits (1431), Expect = 2.4e-154
Identity = 273/356 (76.69%), Postives = 303/356 (85.11%), Query Frame = 0

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLV KDG+YTKQIKT+Y+MITPGQSMD+L+T
Sbjct: 214 MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVAKDGIYTKQIKTHYIMITPGQSMDLLVT 273

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATN 120
           A+ SPG+Y MA +SYSSA GAGFDNTTATAIL YS  PN  LNHFFP+LPPYD T+AAT+
Sbjct: 274 ADQSPGLYFMAARSYSSALGAGFDNTTATAILNYS-KPN-HLNHFFPNLPPYDATKAATD 333

Query: 121 FTKRLRSLT----TVDVPLNVDTRLFFALSVNLIDC-SNDHKPCAGPFGKRFAASINNVS 180
           FTKRLRSLT      DVPLN+DTRLFF LSVNL+ C ++DH+ CAGPFGKRFAASINNVS
Sbjct: 334 FTKRLRSLTIDDRRADVPLNIDTRLFFTLSVNLMSCNASDHRACAGPFGKRFAASINNVS 393

Query: 181 FVTPSVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNAS 240
           FVTPSV++LEA+YN+V GVFTT+F   PPRKF+YTG+ L ENLLATSFGTRV+VLEYNAS
Sbjct: 394 FVTPSVAMLEAYYNDVHGVFTTNFPRKPPRKFDYTGKNLSENLLATSFGTRVIVLEYNAS 453

Query: 241 VEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK------------------ 300
           VE+ILQGTN+LASDNHPVHLHGYSFYVVGWGFGNF+P+TDPK                  
Sbjct: 454 VELILQGTNVLASDNHPVHLHGYSFYVVGWGFGNFNPKTDPKRYNLVNPXEETTVGVPKN 513

Query: 301 -----RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSCY 329
                RFKANNPGMWLMHCHIERHQVWGM MV LVKNGLA HQQIL+PPHDLPSC+
Sbjct: 514 GWVAIRFKANNPGMWLMHCHIERHQVWGMSMVLLVKNGLAPHQQILHPPHDLPSCF 567

BLAST of Lsi03G007930 vs. TAIR 10
Match: AT5G09360.1 (laccase 14 )

HSP 1 Score: 417.5 bits (1072), Expect = 9.7e-117
Identity = 198/348 (56.90%), Postives = 251/348 (72.13%), Query Frame = 0

Query: 3   QGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITAN 62
           +G+ YLLRI+NAVMDE+LFF IA H +T+V KDG Y K  K++YLMITPGQSMD+L+ AN
Sbjct: 226 RGRRYLLRIINAVMDEELFFAIANHTLTVVAKDGFYLKHFKSDYLMITPGQSMDVLLHAN 285

Query: 63  HSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATNFT 122
             P  Y +A ++YSSAFGAGFD TT TAIL+Y      ++    P+LPPY+RTEA+T FT
Sbjct: 286 QRPNHYFVAARAYSSAFGAGFDKTTTTAILQYKGDTLNRIKPILPYLPPYNRTEASTRFT 345

Query: 123 KRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKPCAGPFGKRFAASINNVSFVTPSVS 182
            + RS   V+VP+ ++TRL +A+SVNL++CS+D +PC GPFGKRF++SINN+SFV PSV 
Sbjct: 346 NQFRSQRPVNVPVKINTRLLYAISVNLMNCSDD-RPCTGPFGKRFSSSINNISFVNPSVD 405

Query: 183 LLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVEVILQG 242
           +L A+Y ++GGVF  DF  NPP KFNYTGE LP     T FGT+V+VL+YN+SVE+ILQG
Sbjct: 406 ILRAYYRHIGGVFQEDFPRNPPTKFNYTGENLP---FPTRFGTKVVVLDYNSSVELILQG 465

Query: 243 TNILASDNHPVHLHGYSFYVVGWGFGNFDPETDP-----------------------KRF 302
           T + AS+ HP+HLHGY+FYVVG GFGNFD   DP                        RF
Sbjct: 466 TTVWASNIHPIHLHGYNFYVVGSGFGNFDRRKDPLRYNLVDPPEETTVGVPRNGWTAVRF 525

Query: 303 KANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
            ANNPG+WL+HCHIERH  WGM  VF+VK+G     +++ PP DLPSC
Sbjct: 526 VANNPGVWLLHCHIERHATWGMNTVFIVKDGPTKSSRMVKPPPDLPSC 569

BLAST of Lsi03G007930 vs. TAIR 10
Match: AT2G30210.1 (laccase 3 )

HSP 1 Score: 323.2 bits (827), Expect = 2.5e-88
Identity = 167/355 (47.04%), Postives = 220/355 (61.97%), Query Frame = 0

Query: 4   GKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANH 63
           G+T  LR++NA M+++LFF +A H+ T+V  D  YTK   TN +MI PGQ+ ++L+TAN 
Sbjct: 218 GETVQLRVINAGMNQELFFSVANHQFTVVETDSAYTKPFTTNVIMIGPGQTTNVLLTANQ 277

Query: 64  SPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSP------NPQLNHFFPHLPPYDRTEA 123
            PG Y MA ++Y+SA  A FDNTT TAIL+Y  +P        Q+   FP LP ++ T  
Sbjct: 278 RPGRYYMAARAYNSA-NAPFDNTTTTAILQYVNAPTRRGRGRGQIAPVFPVLPGFNDTAT 337

Query: 124 ATNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKP-CAGPFGKRFAASINNVSF 183
           AT FT RLR      VP  VD  LFF + + LI+C+N + P C GP G RFAAS+NN+SF
Sbjct: 338 ATAFTNRLRYWKRAPVPQQVDENLFFTVGLGLINCANPNSPRCQGPNGTRFAASMNNMSF 397

Query: 184 VTP-SVSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNAS 243
           V P S S+++A+Y    G+FTTDF   PP +F+YTG V    L     GT+   L+Y ++
Sbjct: 398 VLPRSNSVMQAYYQGTPGIFTTDFPPVPPVQFDYTGNV-SRGLWQPIKGTKAYKLKYKSN 457

Query: 244 VEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPKRFK--------------- 303
           V+++LQ T+I+  +NHP+HLHGY FYVVG GFGNF+P TDP RF                
Sbjct: 458 VQIVLQDTSIVTPENHPMHLHGYQFYVVGSGFGNFNPRTDPARFNLFDPPERNTIGTPPG 517

Query: 304 --------ANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
                   A+NPG W MHCHI+ H  WG+ MVFLV+NG    Q +  PP DLP C
Sbjct: 518 GWVAIRFVADNPGAWFMHCHIDSHLGWGLAMVFLVENGRGQLQSVQAPPLDLPRC 570

BLAST of Lsi03G007930 vs. TAIR 10
Match: AT5G05390.1 (laccase 12 )

HSP 1 Score: 310.1 bits (793), Expect = 2.2e-84
Identity = 156/353 (44.19%), Postives = 213/353 (60.34%), Query Frame = 0

Query: 1   MEQGKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILIT 60
           +  G+T LLR++NA +++ LFF +A H++T+VG D  Y K   T  LM+ PGQ+ D+L+T
Sbjct: 214 INSGETSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLMLGPGQTTDVLLT 273

Query: 61  ANHSPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSPNPQLNHFFPHLPPYDRTEAATN 120
           A+  P  Y +A ++Y SA  A FDNTT TAIL+Y  +         P LP ++ T   T+
Sbjct: 274 ADQPPKRYYIAARAYQSAQNAPFDNTTTTAILQYKKTTTTS-KPIMPVLPAFNDTNTVTS 333

Query: 121 FTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSN--DHKPCAGPFGKRFAASINNVSFVT 180
           F+++ +SL  V VP  +D  LFF + + L +C        C G  G RF AS+NNVSFV 
Sbjct: 334 FSRKFKSLRNVVVPKTIDDNLFFTIGLGLDNCPKKFPKSRCQGLNGTRFTASMNNVSFVL 393

Query: 181 PS-VSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNASVE 240
           PS  SLL+A  N + GVFTTDF   PP KF+YTG  +   L     GT++  L+Y + V+
Sbjct: 394 PSNFSLLQAHSNGIPGVFTTDFPSKPPVKFDYTGNNISRALFQPVKGTKLYKLKYGSRVQ 453

Query: 241 VILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK-------------------- 300
           V+LQ TNI+ S+NHP+HLHGY FY+VG GFGNF+P+ D                      
Sbjct: 454 VVLQDTNIVTSENHPIHLHGYDFYIVGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGW 513

Query: 301 ---RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
              RF A+NPG+WLMHCH++ H  WG+ M FLV NG+   + +  PPHDLP C
Sbjct: 514 AVIRFVADNPGVWLMHCHLDVHIKWGLAMAFLVDNGVGELETLEAPPHDLPIC 565

BLAST of Lsi03G007930 vs. TAIR 10
Match: AT5G07130.1 (laccase 13 )

HSP 1 Score: 305.4 bits (781), Expect = 5.4e-83
Identity = 159/356 (44.66%), Postives = 215/356 (60.39%), Query Frame = 0

Query: 4   GKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANH 63
           G+  LLR++N+ ++++LFFG+A H++T+V  D  YTK   TN +M+ PGQ+ D+L+TA+ 
Sbjct: 216 GEIVLLRVINSALNQELFFGVANHKLTVVAADASYTKPFSTNVIMLGPGQTTDVLLTADQ 275

Query: 64  SPGIYIMATKSYSSAFGAGFDNTTATAILKY------STSPNPQLNHFFPHLPPYDRTEA 123
            P  Y MA  +Y+SA  A FDNTT TAILKY      +     Q       LP ++ T  
Sbjct: 276 PPAHYYMAAHAYNSA-NAAFDNTTTTAILKYKDASCVTLQAKSQARAIPAQLPGFNDTAT 335

Query: 124 ATNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDH-KPCAGPFGKRFAASINNVSF 183
           A  FT +++S + V VPL +D  LFF + + L +C   + + C GP G RF ASINNVSF
Sbjct: 336 AAAFTAQMKSPSKVKVPLEIDENLFFTVGLGLFNCPTPNTQRCQGPNGTRFTASINNVSF 395

Query: 184 VTPSV-SLLEAFYNNV-GGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVMVLEYNA 243
           V P   S+++A+Y     GVFTTDF   PP  F+YTG V    L   + GT+   L++N+
Sbjct: 396 VFPKQNSIMQAYYQGTPTGVFTTDFPPTPPVTFDYTGNV-SRGLWQPTRGTKAYKLKFNS 455

Query: 244 SVEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK----------------- 303
            V++ILQ T+I+ ++NHP+HLHGY FYVVG G GNF+P TD                   
Sbjct: 456 QVQIILQDTSIVTTENHPMHLHGYEFYVVGTGVGNFNPNTDTSSFNLIDPPRRNTIGTPP 515

Query: 304 ------RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLPSC 328
                 RF ANNPG WLMHCHI+ H  WG+ MVFLV+NG    Q + +PP DLP C
Sbjct: 516 GGWVAIRFVANNPGAWLMHCHIDSHIFWGLAMVFLVENGEGHLQSVQSPPLDLPQC 569

BLAST of Lsi03G007930 vs. TAIR 10
Match: AT2G40370.1 (laccase 5 )

HSP 1 Score: 303.5 bits (776), Expect = 2.0e-82
Identity = 150/362 (41.44%), Postives = 211/362 (58.29%), Query Frame = 0

Query: 4   GKTYLLRIVNAVMDEDLFFGIAKHEMTLVGKDGLYTKQIKTNYLMITPGQSMDILITANH 63
           G+T LLR++N+ +++ LFF +A H++T+VG D  Y K   TN +++ PGQ+ D+LIT + 
Sbjct: 219 GETILLRVINSALNQPLFFTVANHKLTVVGADASYLKPFTTNVIVLGPGQTTDVLITGDQ 278

Query: 64  SPGIYIMATKSYSSAFGAGFDNTTATAILKYSTSP------------NPQLNHFFPHLPP 123
            P  Y MA ++Y SA  A F NTT TAIL+Y ++P                    P LP 
Sbjct: 279 PPNRYYMAARAYQSAQNAPFGNTTTTAILQYKSAPCCGVGGGSGTKKGNSFKPIMPILPA 338

Query: 124 YDRTEAATNFTKRLRSLTTVDVPLNVDTRLFFALSVNLIDCSNDHKP--CAGPFGKRFAA 183
           Y+ T   T F++  RSL   +VP  +D  LF  + + L +C  + +   C GP G RF A
Sbjct: 339 YNDTNTVTRFSQSFRSLRRAEVPTEIDENLFVTIGLGLNNCPKNFRSRRCQGPNGTRFTA 398

Query: 184 SINNVSFVTPS-VSLLEAFYNNVGGVFTTDFLENPPRKFNYTGEVLPENLLATSFGTRVM 243
           S+NNVSF  PS  SLL+A ++ + GVFTTDF   PP KF+YTG  +  +L     GT++ 
Sbjct: 399 SMNNVSFALPSNYSLLQAHHHGIPGVFTTDFPAKPPVKFDYTGNNISRSLYQPDRGTKLY 458

Query: 244 VLEYNASVEVILQGTNILASDNHPVHLHGYSFYVVGWGFGNFDPETDPK----------- 303
            L+Y + V+++LQ T I+  +NHP+HLHGY FY++  GFGNF+P+ D             
Sbjct: 459 KLKYGSRVQIVLQDTGIVTPENHPIHLHGYDFYIIAEGFGNFNPKKDTAKFNLEDPPLRN 518

Query: 304 ------------RFKANNPGMWLMHCHIERHQVWGMGMVFLVKNGLASHQQILNPPHDLP 328
                       RF A+NPG+W+MHCH++ H  WG+ M FLV+NG    Q I  PPHDLP
Sbjct: 519 TVGVPVNGWAVIRFIADNPGVWIMHCHLDAHISWGLAMAFLVENGNGVLQTIEQPPHDLP 578

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FY791.4e-11556.90Laccase-14 OS=Arabidopsis thaliana OX=3702 GN=LAC14 PE=2 SV=1[more]
Q56YT03.5e-8747.04Laccase-3 OS=Arabidopsis thaliana OX=3702 GN=LAC3 PE=2 SV=2[more]
Q9FLB53.1e-8344.19Laccase-12 OS=Arabidopsis thaliana OX=3702 GN=LAC12 PE=2 SV=1[more]
Q2RBK21.5e-8245.30Putative laccase-17 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC17 PE=3 SV=1[more]
Q9LYQ27.5e-8244.66Laccase-13 OS=Arabidopsis thaliana OX=3702 GN=LAC13 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3C1997.4e-16581.64Laccase OS=Cucumis melo OX=3656 GN=LOC103495685 PE=3 SV=1[more]
A0A6J1DLZ81.2e-15476.69Laccase OS=Momordica charantia OX=3673 GN=LOC111021676 PE=3 SV=1[more]
A0A6J1EPR22.4e-14772.68Laccase OS=Cucurbita moschata OX=3662 GN=LOC111434523 PE=3 SV=1[more]
A0A6J1JN532.1e-14371.55Laccase OS=Cucurbita maxima OX=3661 GN=LOC111485978 PE=3 SV=1[more]
A0A0A0K3Y11.1e-13980.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G058630 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038887094.13.3e-16783.29laccase-14 [Benincasa hispida][more]
XP_008455534.11.5e-16481.64PREDICTED: laccase-14 [Cucumis melo][more]
XP_004137046.17.6e-16482.10laccase-14 [Cucumis sativus][more]
KGN43742.27.6e-16482.10hypothetical protein Csa_017248 [Cucumis sativus][more]
XP_022154399.12.4e-15476.69laccase-14 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G09360.19.7e-11756.90laccase 14 [more]
AT2G30210.12.5e-8847.04laccase 3 [more]
AT5G05390.12.2e-8444.19laccase 12 [more]
AT5G07130.15.4e-8344.66laccase 13 [more]
AT2G40370.12.0e-8241.44laccase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 278..329
e-value: 1.3E-14
score: 56.4
coord: 161..277
e-value: 3.5E-26
score: 94.2
IPR008972CupredoxinGENE3D2.60.40.420coord: 1..131
e-value: 9.0E-29
score: 102.9
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 2..115
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 134..327
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 1..96
e-value: 1.4E-24
score: 87.0
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 203..278
e-value: 3.5E-11
score: 43.0
NoneNo IPR availablePANTHERPTHR11709:SF362LACCASE-14coord: 278..327
coord: 2..279
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 278..327
coord: 2..279
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 290..301
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 285..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G007930.1Lsi03G007930.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032508 DNA duplex unwinding
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0046274 lignin catabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0042555 MCM complex
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0005507 copper ion binding
molecular_function GO:0052716 hydroquinone:oxygen oxidoreductase activity
molecular_function GO:0003697 single-stranded DNA binding
molecular_function GO:0017116 single-stranded DNA helicase activity
molecular_function GO:0016491 oxidoreductase activity