Lsi03G007240 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G007240
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Cystatin domain containing protein
Location	chr03: 9720097 .. 9720471 (+)
RNA-Seq Expression	Lsi03G007240
Synteny	Lsi03G007240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTCTACCGTGCTCGCCAACATCGGTCAACATGATCCCATCCTCCACGTGACAAAGGATACGAAGGGAGATCATCAGTCTGAATGGATATCGATCCGTGCCATCGAAGATCCTTACGTGCAAGAAGTTGCAAAGTTTGCAGTGACAGAGTTTGACAAGGAAAAAATGGCAAAGTTTCAATACAAGCACTTGCTACGTTGTTGGATTAAGGAGGAGATGGGAGGCATAAAATATCGCCTTATTTTAGAGGTAATTAAAGAAGGAAAACTTGAGGGTAAATATGAGGCTATTGTATGGGAGGCTTCTGGCCGTCTGATTATAAGGAAGCTTAAGCTTATATCTTTCATGCCTTGTATTCATGGGGCTGATTGA

mRNA sequence

ATGATTTCTACCGTGCTCGCCAACATCGGTCAACATGATCCCATCCTCCACGTGACAAAGGATACGAAGGGAGATCATCAGTCTGAATGGATATCGATCCGTGCCATCGAAGATCCTTACGTGCAAGAAGTTGCAAAGTTTGCAGTGACAGAGTTTGACAAGGAAAAAATGGCAAAGTTTCAATACAAGCACTTGCTACGTTGTTGGATTAAGGAGGAGATGGGAGGCATAAAATATCGCCTTATTTTAGAGGTAATTAAAGAAGGAAAACTTGAGGGTAAATATGAGGCTATTGTATGGGAGGCTTCTGGCCGTCTGATTATAAGGAAGCTTAAGCTTATATCTTTCATGCCTTGTATTCATGGGGCTGATTGA

Coding sequence (CDS)

ATGATTTCTACCGTGCTCGCCAACATCGGTCAACATGATCCCATCCTCCACGTGACAAAGGATACGAAGGGAGATCATCAGTCTGAATGGATATCGATCCGTGCCATCGAAGATCCTTACGTGCAAGAAGTTGCAAAGTTTGCAGTGACAGAGTTTGACAAGGAAAAAATGGCAAAGTTTCAATACAAGCACTTGCTACGTTGTTGGATTAAGGAGGAGATGGGAGGCATAAAATATCGCCTTATTTTAGAGGTAATTAAAGAAGGAAAACTTGAGGGTAAATATGAGGCTATTGTATGGGAGGCTTCTGGCCGTCTGATTATAAGGAAGCTTAAGCTTATATCTTTCATGCCTTGTATTCATGGGGCTGATTGA

Protein sequence

MISTVLANIGQHDPILHVTKDTKGDHQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEEMGGIKYRLILEVIKEGKLEGKYEAIVWEASGRLIIRKLKLISFMPCIHGAD

Homology

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BLAST of Lsi03G007240 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 2.5e-06
Identity = 32/83 (38.55%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 36  IEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEEMGGIKYRLILEVIKEGKLEGKY 95
           I DP+VQE+ ++AV E D+   A       +    K+ + G+ YRL LE    G  +G+Y
Sbjct: 35  INDPHVQELGRWAVAEEDRGVAAGGLTFERVTDGEKQVVAGVNYRLTLEASSSGAKDGRY 94

Query: 96  EAIVWEASGRLIIRKLKLISFMP 119
           EA+V+E   R   R  KL+SF P
Sbjct: 95  EAVVYEQDPRSNAR--KLVSFEP 115

[Close]

BLAST of Lsi03G007240 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 4.3e-06
Identity = 26/72 (36.11%), Postives = 46/72 (63.89%), Query Frame = 0


Query: 30  WISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEEMGGIKYRLILEVIKEG 89
           W  I ++    VQ+VA+FAV+E +K+   + QY+ ++R +  + + G  YRL++   K+G
Sbjct: 32  WRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGY-TQVVAGTNYRLVI-AAKDG 91

Query: 90  KLEGKYEAIVWE 102
            + G YEA+VW+
Sbjct: 92  AVVGNYEAVVWD 101

[Close]

BLAST of Lsi03G007240 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 9.7e-06
Identity = 26/72 (36.11%), Postives = 45/72 (62.50%), Query Frame = 0


Query: 30  WISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEEMGGIKYRLILEVIKEG 89
           W  I  +    VQ+VA+FAV+E +K+   + QY+ ++R +  + + G  YRL++   K+G
Sbjct: 32  WRPIENLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGY-TQVVAGTNYRLVI-AAKDG 91

Query: 90  KLEGKYEAIVWE 102
            + G YEA+VW+
Sbjct: 92  AVVGNYEAVVWD 101

[Close]

BLAST of Lsi03G007240 vs. ExPASy TrEMBL
Match: A0A5A7SN35 (Cystatin domain containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001920 PE=4 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.1e-56
Identity = 113/123 (91.87%), Postives = 117/123 (95.12%), Query Frame = 0


Query: 1   MISTVLANIGQHDPILHVT-KDTKGDHQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAK 60
           MISTVLANIGQHDPILHVT KDTKGDHQSEWISIRAIEDPYVQEVAKFAV EF+KEKMA+
Sbjct: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60

Query: 61  FQYKHLLRCWIKEEMGGIKYRLILEVIKEGKLEGKYEAIVWEASGRLIIRKLKLISFMPC 120
            QYK LL CWIKEEMGGIKYRLILEVIKE K+EGKYEAIVWEASGRLIIRKLKLISFMPC
Sbjct: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120

Query: 121 IHG 123
           +HG
Sbjct: 121 MHG 123

[Close]

BLAST of Lsi03G007240 vs. ExPASy TrEMBL
Match: A0A0A0K644 (Cystatin domain containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G066240 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 6.2e-56
Identity = 110/124 (88.71%), Postives = 118/124 (95.16%), Query Frame = 0


Query: 1   MISTVLANIGQHDPILHVT-KDTKGDHQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAK 60
           MIST+LAN+GQHDPILHVT KDTKGDHQSEWISIRAIEDPYVQEVAKFA+ EF+KEKMA+
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKHLLRCWIKEEMGGIKYRLILEVIKEGKLEGKYEAIVWEASGRLIIRKLKLISFMPC 120
           FQYK LL CWIKEEMGGIKYRL+LEVIKE KLEGKYEA+VWEASGRLIIRKLKLISFMP 
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 IHGA 124
           +HGA
Sbjct: 121 MHGA 124

[Close]

BLAST of Lsi03G007240 vs. ExPASy TrEMBL
Match: A0A6J1IC35 (cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 4.8e-08
Identity = 35/105 (33.33%), Postives = 64/105 (60.95%), Query Frame = 0


Query: 14  PILHVTKDTKGDHQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEE 73
           P+L V     G     W  I+ ++DP++QE+ KFAV+E++K+     ++K +++    + 
Sbjct: 15  PLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQV 74

Query: 74  MGGIKYRLILEVIKEGKLEGKYEAIVWEASGRLIIRKLKLISFMP 119
           + G+ YRL++E  K+G+  GKY+A+VWE + +     ++L SF P
Sbjct: 75  VSGMNYRLVIEA-KKGESIGKYQALVWEKAWQ---HFMELTSFKP 114

[Close]

BLAST of Lsi03G007240 vs. ExPASy TrEMBL
Match: A0A6J1EKC1 (cysteine proteinase inhibitor 5-like OS=Cucurbita moschata OX=3662 GN=LOC111434113 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 6.3e-08
Identity = 34/105 (32.38%), Postives = 64/105 (60.95%), Query Frame = 0


Query: 14  PILHVTKDTKGDHQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEE 73
           P+L V     G     W  I+ ++DP++QE+ KFAV+E++K+     ++K +++    + 
Sbjct: 15  PLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQV 74

Query: 74  MGGIKYRLILEVIKEGKLEGKYEAIVWEASGRLIIRKLKLISFMP 119
           + G+ YRL++E  K+G+  GKY+A++WE + +     ++L SF P
Sbjct: 75  VSGMNYRLVIEA-KKGESIGKYQALIWEKAWQ---HFMELTSFKP 114

[Close]

BLAST of Lsi03G007240 vs. ExPASy TrEMBL
Match: A0A2P2NS36 (Cysteine proteinase inhibitor OS=Rhizophora mucronata OX=61149 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 4.1e-07
Identity = 36/89 (40.45%), Postives = 54/89 (60.67%), Query Frame = 0


Query: 30  WISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEEMGGIKYRLILEVIKEG 89
           W  I  + DP+VQE+A +AV E++    A  + + L++   K+ + GIKYRL+L  +KEG
Sbjct: 32  WTPITNLSDPHVQEIASYAVREYNHRSNANLKLQRLVK-GEKQVVSGIKYRLVL-AVKEG 91

Query: 90  KLEGKYEAIVWEASGRLIIRKLKLISFMP 119
             + KY+A+VWE +        KLISF P
Sbjct: 92  GSDKKYQAVVWEKAWE---HFRKLISFKP 115

[Close]

BLAST of Lsi03G007240 vs. NCBI nr
Match: KAA0031436.1 (Cystatin domain containing protein [Cucumis melo var. makuwa] >TYK06887.1 Cystatin domain containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 228.0 bits (580), Expect = 4.4e-56
Identity = 113/123 (91.87%), Postives = 117/123 (95.12%), Query Frame = 0


Query: 1   MISTVLANIGQHDPILHVT-KDTKGDHQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAK 60
           MISTVLANIGQHDPILHVT KDTKGDHQSEWISIRAIEDPYVQEVAKFAV EF+KEKMA+
Sbjct: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60

Query: 61  FQYKHLLRCWIKEEMGGIKYRLILEVIKEGKLEGKYEAIVWEASGRLIIRKLKLISFMPC 120
            QYK LL CWIKEEMGGIKYRLILEVIKE K+EGKYEAIVWEASGRLIIRKLKLISFMPC
Sbjct: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120

Query: 121 IHG 123
           +HG
Sbjct: 121 MHG 123

[Close]

BLAST of Lsi03G007240 vs. NCBI nr
Match: KGN43767.1 (hypothetical protein Csa_017208 [Cucumis sativus])

HSP 1 Score: 226.5 bits (576), Expect = 1.3e-55
Identity = 110/124 (88.71%), Postives = 118/124 (95.16%), Query Frame = 0


Query: 1   MISTVLANIGQHDPILHVT-KDTKGDHQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAK 60
           MIST+LAN+GQHDPILHVT KDTKGDHQSEWISIRAIEDPYVQEVAKFA+ EF+KEKMA+
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKHLLRCWIKEEMGGIKYRLILEVIKEGKLEGKYEAIVWEASGRLIIRKLKLISFMPC 120
           FQYK LL CWIKEEMGGIKYRL+LEVIKE KLEGKYEA+VWEASGRLIIRKLKLISFMP 
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 IHGA 124
           +HGA
Sbjct: 121 MHGA 124

[Close]

BLAST of Lsi03G007240 vs. NCBI nr
Match: KAG6571859.1 (hypothetical protein SDJN03_28587, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011544.1 hypothetical protein SDJN02_26450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 201.4 bits (511), Expect = 4.4e-48
Identity = 99/120 (82.50%), Postives = 105/120 (87.50%), Query Frame = 0


Query: 5   VLANIGQHDPILHVTKDTKGDHQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKH 64
           +LANIGQHDPILHVTKD KGDH SEWISIRAIEDPYVQEVAKF V E +KEK A FQYK 
Sbjct: 1   MLANIGQHDPILHVTKDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKG 60

Query: 65  LLRCWIKEEMGGIKYRLILEVIKEGKLEGKYEAIVWEASGRLIIRKLKLISFMPCIHGAD 124
           LLRCW+KEEMGGIKYRLILEV K G  EGKYEAIVWEASGRLIIRKLKL+SF+ C HG +
Sbjct: 61  LLRCWVKEEMGGIKYRLILEV-KGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHGVE 119

[Close]

BLAST of Lsi03G007240 vs. NCBI nr
Match: KAG6588760.1 (hypothetical protein SDJN03_17325, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 193.7 bits (491), Expect = 9.2e-46
Identity = 94/121 (77.69%), Postives = 105/121 (86.78%), Query Frame = 0


Query: 1   MISTVLANIGQHDPILHVTKDTKGDHQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAKF 60
           MIST+L NIGQHDPILHVTKD KGD +SEWISIR IEDPYVQEVAKFAV EF+ +KM  F
Sbjct: 1   MISTLLTNIGQHDPILHVTKDVKGDPRSEWISIRTIEDPYVQEVAKFAVIEFNVDKMTTF 60

Query: 61  QYKHLLRCWIKEEMGGIKYRLILEVIKEGKLEG--KYEAIVWEASGRLIIRKLKLISFMP 120
           QYK +LRCWIKEEMGGIKYRL+LEV +E   +   KYEA+VWEASGRLIIRKLKL+SF+P
Sbjct: 61  QYKRMLRCWIKEEMGGIKYRLVLEVKQEAPTQKVCKYEAVVWEASGRLIIRKLKLVSFVP 120

[Close]

BLAST of Lsi03G007240 vs. NCBI nr
Match: XP_022973098.1 (cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine proteinase inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 67.4 bits (163), Expect = 9.9e-08
Identity = 35/105 (33.33%), Postives = 64/105 (60.95%), Query Frame = 0


Query: 14  PILHVTKDTKGDHQSEWISIRAIEDPYVQEVAKFAVTEFDKEKMAKFQYKHLLRCWIKEE 73
           P+L V     G     W  I+ ++DP++QE+ KFAV+E++K+     ++K +++    + 
Sbjct: 15  PLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQV 74

Query: 74  MGGIKYRLILEVIKEGKLEGKYEAIVWEASGRLIIRKLKLISFMP 119
           + G+ YRL++E  K+G+  GKY+A+VWE + +     ++L SF P
Sbjct: 75  VSGMNYRLVIEA-KKGESIGKYQALVWEKAWQ---HFMELTSFKP 114

The following BLAST results are available for this feature:

BLAST of Lsi03G007240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q10Q47	2.5e-06	38.55	Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q6TPK4	4.3e-06	36.11	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
P86472	9.7e-06	36.11	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]

BLAST of Lsi03G007240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SN35	2.1e-56	91.87	Cystatin domain containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A0A0K644	6.2e-56	88.71	Cystatin domain containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G066240 PE...	[more]
A0A6J1IC35	4.8e-08	33.33	cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628...	[more]
A0A6J1EKC1	6.3e-08	32.38	cysteine proteinase inhibitor 5-like OS=Cucurbita moschata OX=3662 GN=LOC1114341...	[more]
A0A2P2NS36	4.1e-07	40.45	Cysteine proteinase inhibitor OS=Rhizophora mucronata OX=61149 PE=4 SV=1	[more]

BLAST of Lsi03G007240 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0031436.1	4.4e-56	91.87	Cystatin domain containing protein [Cucumis melo var. makuwa] >TYK06887.1 Cystat...	[more]
KGN43767.1	1.3e-55	88.71	hypothetical protein Csa_017208 [Cucumis sativus]	[more]
KAG6571859.1	4.4e-48	82.50	hypothetical protein SDJN03_28587, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6588760.1	9.2e-46	77.69	hypothetical protein SDJN03_17325, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022973098.1	9.9e-08	33.33	cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine p...	[more]

BLAST of Lsi03G007240 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 33..104 e-value: 4.4E-13 score: 49.4
None	No IPR available	GENE3D	3.10.450.10		coord: 24..119 e-value: 2.4E-15 score: 58.5
None	No IPR available	PANTHER	PTHR47364	CYSTEINE PROTEINASE INHIBITOR 5	coord: 21..117
None	No IPR available	PANTHER	PTHR47364:SF2	CYSTEINE PROTEINASE INHIBITOR 5	coord: 21..117
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 30..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G007240.1	Lsi03G007240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity