Lsi03G006730 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi03G006730 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 7.0e-83
Identity = 152/338 (44.97%), Postives = 207/338 (61.24%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNLIRENLNTP---FSHLPYGETFFNNPTGRCSNGLLMIDYFALDA 61
           FD+IYQLGDS SDTGNLIR   + P    +H PYGETF   PTGRCS+G L+ID+ A   
Sbjct: 27  FDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYGETFPGTPTGRCSDGRLIIDFIATAL 86

Query: 62  GLPFVNPYLNKDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHFN 121
            LP +NPYL ++    HGVNFAVAG+TAL    L+ + +     +S L+ QL+W  ++  
Sbjct: 87  NLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLAARGVQVSDIHSHLSAQLNWFRTYLG 146

Query: 122 SICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVI 181
           SIC   +EC+ KL NALF++G IG ND NYA F  + I+E++  VP + +++ +A   +I
Sbjct: 147 SICSTPKECSNKLKNALFILGNIGNNDVNYA-FPNRTIEEIRAYVPFITEAVANATREII 206

Query: 182 SYGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKAL 241
             G +RV+VPG FPIGC    L           D+L CL  LN+ + Y N   +  + +L
Sbjct: 207 RLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDDLGCLSSLNNLSIYFNSLFQRALASL 266

Query: 242 KKENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVE 301
             E P  VI+Y DYYNA+ ++ R    LG +  SL K CCG+GG YN++  + CG  GV 
Sbjct: 267 SIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSLLKCCCGIGGPYNYDPDRECGSRGVP 326

Query: 302 VCENPNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHC 337
           VC NP ++I WDG H TQ AY+ +A ++I  I   L C
Sbjct: 327 VCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIKALKC 363

BLAST of Lsi03G006730 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 295.8 bits (756), Expect = 6.1e-79
Identity = 148/331 (44.71%), Postives = 217/331 (65.56%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNL--IRENLNTPFSHL-PYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F +I   GDSI+DTGNL  + +  + P S   PYGETFF++PTGR S+G L+ID+ A   
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFL 93

Query: 62  GLPFVNPYLN-KDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHF 121
           G P V P+   ++A    GVNFAVAG+TAL    L ++ I S +TN SL+ QL       
Sbjct: 94  GFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESL 153

Query: 122 NSICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERV 181
            ++C +  +C + + NAL L+GEIGGNDYN+ALFQ K ++EV+++VP V+ +I SA+  +
Sbjct: 154 PNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITEL 213

Query: 182 ISYGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDEL-HCLKDLNSFASYHNDQIKEMIK 241
           +  G    +VPGNFPIG    YLT ++T++   YD L  CLK LN F+ Y+N Q++E + 
Sbjct: 214 VCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELN 273

Query: 242 ALKKENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLG 301
            L+K  PH  I+Y DYYNA L + +     GF    L  +CCGVGG+YNFN  + CG +G
Sbjct: 274 GLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PACCGVGGSYNFNFSRRCGSVG 333

Query: 302 VEVCENPNEHISWDGVHLTQKAYKFMAHWLI 328
           VE C++P++++++DG+H+T+ AY+ ++  L+
Sbjct: 334 VEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

BLAST of Lsi03G006730 vs. ExPASy Swiss-Prot
Match: Q9C857 (GDSL esterase/lipase At1g31550 OS=Arabidopsis thaliana OX=3702 GN=At1g31550 PE=2 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 8.9e-78
Identity = 152/331 (45.92%), Postives = 210/331 (63.44%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNL--IRENLNTPFSHL-PYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F++I   GDSI+DTGNL  + ++ N P S   PYGETFF++PTGR S+G L+ID+ A   
Sbjct: 34  FESIISFGDSIADTGNLLGLSDHNNLPMSAFPPYGETFFHHPTGRFSDGRLIIDFIAEFL 93

Query: 62  GLPFVNPYL-NKDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHF 121
           GLP+V PY  + +     GVNFAVA +TAL S  L +K    P  N SL  QL       
Sbjct: 94  GLPYVPPYFGSTNGNFEKGVNFAVASATALESSFLEEKGYHCP-HNFSLGVQLKIFKQSL 153

Query: 122 NSICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERV 181
            ++C    +C + + NAL L+GEIG NDYN+  FQ + + EVK++VP V+ +I SA+  +
Sbjct: 154 PNLCGLPSDCRDMIGNALILMGEIGANDYNFPFFQLRPLDEVKELVPLVISTISSAITEL 213

Query: 182 ISYGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDEL-HCLKDLNSFASYHNDQIKEMIK 241
           I  G    +VPG FP+GC   +LT  QT++   YD L  CLK LN F  YH++Q++E + 
Sbjct: 214 IGMGGRTFLVPGGFPLGCSVAFLTLHQTSNMEEYDPLTGCLKWLNKFGEYHSEQLQEELN 273

Query: 242 ALKKENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLG 301
            L+K NPH  I+Y DYYNA L + R     GF    L  +CCGVGG YNFNL + CG +G
Sbjct: 274 RLRKLNPHVNIIYADYYNASLRLGREPSKYGFINRHL-SACCGVGGPYNFNLSRSCGSVG 333

Query: 302 VEVCENPNEHISWDGVHLTQKAYKFMAHWLI 328
           VE C +P+++++WDG+H+T+ A+K MA  L+
Sbjct: 334 VEACSDPSKYVAWDGLHMTEAAHKSMADGLV 362

BLAST of Lsi03G006730 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.5e-77
Identity = 147/331 (44.41%), Postives = 210/331 (63.44%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNL--IRENLNTP-FSHLPYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F +I   GDSI+DTGNL  + +  N P  + LPYGETFF++PTGR SNG L+ID+ A   
Sbjct: 32  FKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHPTGRFSNGRLIIDFIAEFL 91

Query: 62  GLPFVNP-YLNKDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHF 121
           G P V P Y +++A    GVNFAV G+TAL    L ++ I  P TN SL  QL       
Sbjct: 92  GFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLEERGIHFPYTNVSLAVQLSSFKESL 151

Query: 122 NSICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERV 181
            ++C +  +C + + N+L L+GEIGGNDYNYA F GKNI+E+K++VP V+++I SA+  +
Sbjct: 152 PNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVIETISSAITEL 211

Query: 182 ISYGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDEL-HCLKDLNSFASYHNDQIKEMIK 241
           I  G    +VPG FP+GC   YL+ +QT++   YD L  CLK LN F+ YH++Q++  + 
Sbjct: 212 IGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELN 271

Query: 242 ALKKENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLG 301
            L+K  PH  I+Y DYYN  L + +     GF    L  +CC +GG +NF L +  G   
Sbjct: 272 RLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRPL-PACCALGGPFNFTLGRKRGTQV 331

Query: 302 VEVCENPNEHISWDGVHLTQKAYKFMAHWLI 328
            E C++P++++SWDGVH+T+ AY+ MA  ++
Sbjct: 332 PECCDDPSKYVSWDGVHMTEAAYRLMAEGIL 361

BLAST of Lsi03G006730 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.5e-77
Identity = 154/334 (46.11%), Postives = 200/334 (59.88%), Query Frame = 0

Query: 3   DAIYQLGDSISDTGNLIRENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGLPF 62
           ++IYQ GDSISDTGNLIR                 N P                 A  P 
Sbjct: 28  NSIYQFGDSISDTGNLIR-----------------NGP-----------------ASSPT 87

Query: 63  VNPYLNKDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHFNSICY 122
             P   ++   N  VNF V+GSTAL S   S++ +  P TN+ L+ QL W   H  S C+
Sbjct: 88  PKPLPQRE--HNVFVNFGVSGSTALNSSFFSERNLHVPATNTPLSMQLAWFKGHLRSTCH 147

Query: 123 NQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVISYGA 182
                ++ L ++LF+VGEIGGNDYNY  FQGK ++E++  +P VV +I +A   VI  GA
Sbjct: 148 G--SSSDCLKHSLFMVGEIGGNDYNYGFFQGKPMEEIRSYIPHVVGAITAAAREVIRAGA 207

Query: 183 TRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKALKKEN 242
             VVVPGNFP+GCFPIYLT F   DT  YD+  CL  LN FA  HN+Q++E I +L+KE 
Sbjct: 208 VNVVVPGNFPVGCFPIYLTSFPVKDTKDYDDNGCLTHLNEFAMDHNNQLQEAIASLRKEF 267

Query: 243 PHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVEVCEN 302
           P   IVYGDYYNAF ++LR      FD+    KSCCG GG YN++  +  G +GV VC+N
Sbjct: 268 PDVAIVYGDYYNAFQYVLRSE---RFDKSVALKSCCGTGGAYNYDGKRPYGAVGVPVCQN 320

Query: 303 PNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHC 337
           P++ ISWDGVHLTQKAY+FM+ +L + I  ++ C
Sbjct: 328 PHKFISWDGVHLTQKAYRFMSKFLNNQILSQIKC 320

BLAST of Lsi03G006730 vs. ExPASy TrEMBL
Match: A0A1S4E629 (acetylajmalan esterase-like OS=Cucumis melo OX=3656 GN=LOC103503259 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.2e-175
Identity = 289/339 (85.25%), Postives = 312/339 (92.04%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIREN NTPFSHLPYG++FFNNPTGRCSNGLLM+D+FALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 91

Query: 61  PFVNPYLNKDALTNHGVNFAVAGSTALPSELLSQK-KISSPVTNSSLNHQLDWMFSHFNS 120
           P V+PYLNKD L +HGVNFAVAGSTAL S+ LS K KI SPVTNSSL+HQL+WMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNS 151

Query: 121 ICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVIS 180
           IC+NQR+C EKL +ALF VGEIGGNDYNYALFQGK IQE KDMVPDVVQ+IKSAVE+VIS
Sbjct: 152 ICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKALK 240
           YGATRVVVPGNFPIGCFPIYLTGF TNDT+AYDELHCLKDLN FA+YHNDQIK+ I+ LK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATYHNDQIKQTIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVEV 300
           KENPHTVIVYGDYYNAFLWI+R A VLGFDE SLQKSCCG+GG+YNFNLM+MCG+ GVE 
Sbjct: 272 KENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEA 331

Query: 301 CENPNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHCIV 339
           C NPNEHISWDGVHLTQK YKFM HWLIHDIF KLHCIV
Sbjct: 332 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

BLAST of Lsi03G006730 vs. ExPASy TrEMBL
Match: A0A5D3CNR6 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold745G00130 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.4e-174
Identity = 288/339 (84.96%), Postives = 312/339 (92.04%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIREN NTPFSHLPYG++FFNNPTGRCSNGLLM+D+FALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 91

Query: 61  PFVNPYLNKDALTNHGVNFAVAGSTALPSELLSQK-KISSPVTNSSLNHQLDWMFSHFNS 120
           P V+PYLNKD L +HGVNFAVAGSTAL S+ LS K KI SPVTNSSL+HQL+WMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNS 151

Query: 121 ICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVIS 180
           IC+NQR+C EKL +ALF VGEIGGNDYNYALFQGK IQE KDMVPDVVQ+IKSAVE+VIS
Sbjct: 152 ICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKALK 240
           YGATRVVVPGNFPIGCFPIYLTGF TNDT+AYDELHCLKDLN FA++HNDQIK+ I+ LK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATHHNDQIKQTIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVEV 300
           KENPHTVIVYGDYYNAFLWI+R A VLGFDE SLQKSCCG+GG+YNFNLM+MCG+ GVE 
Sbjct: 272 KENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEA 331

Query: 301 CENPNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHCIV 339
           C NPNEHISWDGVHLTQK YKFM HWLIHDIF KLHCIV
Sbjct: 332 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

BLAST of Lsi03G006730 vs. ExPASy TrEMBL
Match: A0A6J1KFS3 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493492 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 5.2e-174
Identity = 289/338 (85.50%), Postives = 311/338 (92.01%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIR+N NTPFSHLPYGET FN  TGRCSNGLLMIDYFALDAGL
Sbjct: 37  MFDAIYQLGDSISDTGNLIRQNPNTPFSHLPYGETLFNKSTGRCSNGLLMIDYFALDAGL 96

Query: 61  PFVNPYLNKDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHFNSI 120
           P VNPYLNKDALT HGVNFAVAGSTAL S+LLSQK+I SPVTNSSLN QL WMFSHFNSI
Sbjct: 97  PLVNPYLNKDALTRHGVNFAVAGSTALSSQLLSQKQILSPVTNSSLNQQLYWMFSHFNSI 156

Query: 121 CYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVISY 180
           CYNQR+C EKL NALFLVGEIGGNDYNYALFQGK IQ+VKDMVP VVQ+IK+AVE+VISY
Sbjct: 157 CYNQRDCNEKLRNALFLVGEIGGNDYNYALFQGKTIQDVKDMVPQVVQTIKNAVEKVISY 216

Query: 181 GATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKALKK 240
           GATRVVVPGNFPIGC PIYLTGFQTNDTTAYDELHCLKDLN  A+YHNDQIKE I+ LK+
Sbjct: 217 GATRVVVPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKDLNGLATYHNDQIKEAIEVLKR 276

Query: 241 ENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVEVC 300
           ENPHT+IVYGDYYNA LWILRRAF+LGFDE SLQKSCCG+GG+YNF+LMKMCG+ GV VC
Sbjct: 277 ENPHTMIVYGDYYNALLWILRRAFILGFDETSLQKSCCGIGGDYNFSLMKMCGVGGVSVC 336

Query: 301 ENPNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHCIV 339
            NP+E +SWDG+HLTQKAYKFMA+WLIHDI  +LHCIV
Sbjct: 337 XNPDERVSWDGIHLTQKAYKFMAYWLIHDILPQLHCIV 374

BLAST of Lsi03G006730 vs. ExPASy TrEMBL
Match: A0A5A7SSW2 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold219G001390 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 5.2e-174
Identity = 288/339 (84.96%), Postives = 311/339 (91.74%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIREN NTPFSHLPYG++FFNNPTGRCSNGLLM+D+FALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 91

Query: 61  PFVNPYLNKDALTNHGVNFAVAGSTALPSELLSQK-KISSPVTNSSLNHQLDWMFSHFNS 120
           P V+PYLNKD L +HGVNFAVAGSTAL S+ LS K KI SPVTNSSL+HQL+WMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNS 151

Query: 121 ICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVIS 180
           IC+NQR+C EKL +ALF VGEIGGNDYNYALFQGK IQE KDMVPDVVQ+IKSAVE+VIS
Sbjct: 152 ICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKALK 240
           YGATRVVVPGNFPIGCFPIYLTGF TNDT+AYDELHCLKDLN FA+YHNDQIK+ I+ LK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATYHNDQIKQTIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVEV 300
           KENPHTVIVYGDYYNAFLWI+R A VLGFDE SLQKSCCG+GG+YNFNLM+MCG+ GVE 
Sbjct: 272 KENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEA 331

Query: 301 CENPNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHCIV 339
           C NPNEHISWD VHLTQK YKFM HWLIHDIF KLHCIV
Sbjct: 332 CPNPNEHISWDRVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

BLAST of Lsi03G006730 vs. ExPASy TrEMBL
Match: A0A6J1K9I7 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493500 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.2e-173
Identity = 288/338 (85.21%), Postives = 310/338 (91.72%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIR+N NTPFSHLPYGET FN  TGRCSNGLLMIDYFALDAGL
Sbjct: 37  MFDAIYQLGDSISDTGNLIRQNPNTPFSHLPYGETLFNKSTGRCSNGLLMIDYFALDAGL 96

Query: 61  PFVNPYLNKDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHFNSI 120
           P VNPYLNKDALT HGVNFAVAGSTAL S+LLSQK+I SPVTNSSLN QL WMFSHFNSI
Sbjct: 97  PLVNPYLNKDALTRHGVNFAVAGSTALSSQLLSQKQILSPVTNSSLNQQLYWMFSHFNSI 156

Query: 121 CYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVISY 180
           CYNQR+C EKL NALFLVGEIGGNDYNYALFQGK IQ+VKDMVP VVQ+IK+AVE+ ISY
Sbjct: 157 CYNQRDCNEKLRNALFLVGEIGGNDYNYALFQGKTIQDVKDMVPQVVQTIKNAVEKAISY 216

Query: 181 GATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKALKK 240
           GATRVVVPGNFPIGC PIYLTGFQTNDTTAYDELHCLKDLN  A+YHNDQIKE I+ LK+
Sbjct: 217 GATRVVVPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKDLNGLATYHNDQIKEAIEVLKR 276

Query: 241 ENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVEVC 300
           ENPHT+IVYGDYYNA LWILRRAF+LGFDE SLQKSCCG+GG+YNF+LMKMCG+ GV VC
Sbjct: 277 ENPHTMIVYGDYYNALLWILRRAFILGFDETSLQKSCCGIGGDYNFSLMKMCGVGGVSVC 336

Query: 301 ENPNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHCIV 339
            NP+E +SWDG+HLTQKAYKFMA+WLIHDI  +LHCIV
Sbjct: 337 SNPDERVSWDGIHLTQKAYKFMAYWLIHDILPQLHCIV 374

BLAST of Lsi03G006730 vs. NCBI nr
Match: XP_038877043.1 (LOW QUALITY PROTEIN: GDSL esterase/lipase At5g03980-like, partial [Benincasa hispida])

HSP 1 Score: 663.7 bits (1711), Expect = 8.5e-187
Identity = 312/337 (92.58%), Postives = 323/337 (95.85%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGE+FFNNPTGRCSNGLLMIDYFALDAGL
Sbjct: 31  MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGESFFNNPTGRCSNGLLMIDYFALDAGL 90

Query: 61  PFVNPYLNKDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHFNSI 120
           P V+PYLNKDALTNHGVNFAVAGSTAL SELL QKKISSPVTNSSL+HQLDWMFSHFNSI
Sbjct: 91  PLVSPYLNKDALTNHGVNFAVAGSTALSSELLFQKKISSPVTNSSLSHQLDWMFSHFNSI 150

Query: 121 CYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVISY 180
           CYNQR+C EKL+NALFLVGEIGGNDYNYALFQGK IQEVKDMVPDVVQSIKSAVERVISY
Sbjct: 151 CYNQRDCNEKLYNALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQSIKSAVERVISY 210

Query: 181 GATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKALKK 240
           GATRVVVPGNFPIGCFPIYLT FQTNDT+AYDELHCLKDLN FASYHNDQ+KE I+ LKK
Sbjct: 211 GATRVVVPGNFPIGCFPIYLTAFQTNDTSAYDELHCLKDLNGFASYHNDQVKEAIEVLKK 270

Query: 241 ENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVEVC 300
           ENP TVIVYGDYYNAFLWILR AFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGL GVEVC
Sbjct: 271 ENPXTVIVYGDYYNAFLWILRHAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLPGVEVC 330

Query: 301 ENPNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHCI 338
           +NPNEHISWDGVHLTQKAYKFMAHWLIH IF +LHC+
Sbjct: 331 QNPNEHISWDGVHLTQKAYKFMAHWLIHAIFPELHCL 367

BLAST of Lsi03G006730 vs. NCBI nr
Match: XP_011659746.1 (acetylajmalan esterase-like [Cucumis sativus])

HSP 1 Score: 625.9 bits (1613), Expect = 2.0e-175
Identity = 291/339 (85.84%), Postives = 312/339 (92.04%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIREN NTPFSHLPYG++FFNNPTGRCSNGLLM+D+ ALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFLALDAGL 91

Query: 61  PFVNPYLNKDALTNHGVNFAVAGSTALPSELLSQK-KISSPVTNSSLNHQLDWMFSHFNS 120
           P V+PYLNKD L +HGVNFAVAGSTALPS+ LS   KI SPVTNSSL+HQLDWMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNS 151

Query: 121 ICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVIS 180
           IC+NQREC EKL +ALFLVGEIGGNDYNYALFQGK IQE KDMVPDVVQ+IKSAVE+VIS
Sbjct: 152 ICHNQRECNEKLRSALFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKALK 240
           YGATRVVVPGNFPIGCFPIYLTGF TNDT+AYDELHCLKDLNSFA+YHNDQIK+ I+ LK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNSFATYHNDQIKQAIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVEV 300
           KENPH VIVYGDYYNAFLWI+R AFVLGFDE SLQKSCCG+GG+Y FNLM+MCG+ GVE 
Sbjct: 272 KENPHAVIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEA 331

Query: 301 CENPNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHCIV 339
           C NPNEHISWDGVHLTQK YKFM HWLIHDIF KLHCIV
Sbjct: 332 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

BLAST of Lsi03G006730 vs. NCBI nr
Match: KAE8646130.1 (hypothetical protein Csa_016115 [Cucumis sativus])

HSP 1 Score: 625.9 bits (1613), Expect = 2.0e-175
Identity = 291/339 (85.84%), Postives = 312/339 (92.04%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIREN NTPFSHLPYG++FFNNPTGRCSNGLLM+D+ ALDAGL
Sbjct: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFLALDAGL 60

Query: 61  PFVNPYLNKDALTNHGVNFAVAGSTALPSELLSQK-KISSPVTNSSLNHQLDWMFSHFNS 120
           P V+PYLNKD L +HGVNFAVAGSTALPS+ LS   KI SPVTNSSL+HQLDWMFSHFNS
Sbjct: 61  PLVSPYLNKDGLMDHGVNFAVAGSTALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNS 120

Query: 121 ICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVIS 180
           IC+NQREC EKL +ALFLVGEIGGNDYNYALFQGK IQE KDMVPDVVQ+IKSAVE+VIS
Sbjct: 121 ICHNQRECNEKLRSALFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 180

Query: 181 YGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKALK 240
           YGATRVVVPGNFPIGCFPIYLTGF TNDT+AYDELHCLKDLNSFA+YHNDQIK+ I+ LK
Sbjct: 181 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNSFATYHNDQIKQAIEVLK 240

Query: 241 KENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVEV 300
           KENPH VIVYGDYYNAFLWI+R AFVLGFDE SLQKSCCG+GG+Y FNLM+MCG+ GVE 
Sbjct: 241 KENPHAVIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEA 300

Query: 301 CENPNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHCIV 339
           C NPNEHISWDGVHLTQK YKFM HWLIHDIF KLHCIV
Sbjct: 301 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 339

BLAST of Lsi03G006730 vs. NCBI nr
Match: XP_016903430.1 (PREDICTED: acetylajmalan esterase-like [Cucumis melo])

HSP 1 Score: 623.2 bits (1606), Expect = 1.3e-174
Identity = 289/339 (85.25%), Postives = 312/339 (92.04%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIREN NTPFSHLPYG++FFNNPTGRCSNGLLM+D+FALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 91

Query: 61  PFVNPYLNKDALTNHGVNFAVAGSTALPSELLSQK-KISSPVTNSSLNHQLDWMFSHFNS 120
           P V+PYLNKD L +HGVNFAVAGSTAL S+ LS K KI SPVTNSSL+HQL+WMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNS 151

Query: 121 ICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVIS 180
           IC+NQR+C EKL +ALF VGEIGGNDYNYALFQGK IQE KDMVPDVVQ+IKSAVE+VIS
Sbjct: 152 ICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKALK 240
           YGATRVVVPGNFPIGCFPIYLTGF TNDT+AYDELHCLKDLN FA+YHNDQIK+ I+ LK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATYHNDQIKQTIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVEV 300
           KENPHTVIVYGDYYNAFLWI+R A VLGFDE SLQKSCCG+GG+YNFNLM+MCG+ GVE 
Sbjct: 272 KENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEA 331

Query: 301 CENPNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHCIV 339
           C NPNEHISWDGVHLTQK YKFM HWLIHDIF KLHCIV
Sbjct: 332 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

BLAST of Lsi03G006730 vs. NCBI nr
Match: XP_023546436.1 (acetylajmalan esterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 622.1 bits (1603), Expect = 2.9e-174
Identity = 290/338 (85.80%), Postives = 311/338 (92.01%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIR+N NTPFS+LPYGETFFN  TGRCSNGLLMIDYFALDAGL
Sbjct: 37  MFDAIYQLGDSISDTGNLIRQNPNTPFSNLPYGETFFNKSTGRCSNGLLMIDYFALDAGL 96

Query: 61  PFVNPYLNKDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHFNSI 120
           P VNPYLNKDALT HGVNFAVAGSTAL S+LLSQ +I SP+TNSSLN QL WMFSHFNSI
Sbjct: 97  PLVNPYLNKDALTRHGVNFAVAGSTALSSQLLSQNQILSPLTNSSLNQQLYWMFSHFNSI 156

Query: 121 CYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVISY 180
           CYNQR+C +KL NALFLVGEIGGNDYNYALFQGK IQEVKDMVP VVQ+IKSAVE+VISY
Sbjct: 157 CYNQRDCNQKLRNALFLVGEIGGNDYNYALFQGKTIQEVKDMVPQVVQTIKSAVEKVISY 216

Query: 181 GATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKALKK 240
           GATRVVVPGNFPIGC PIYLTGFQTNDTTAYDELHCLKDLN  A+YHNDQIK+ I+ LK+
Sbjct: 217 GATRVVVPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKDLNGLATYHNDQIKQAIEVLKR 276

Query: 241 ENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVEVC 300
           ENPHTVIVYGDYYNA LWILRRAF LGFDE SLQKSCCG+GGNYNF+LMKMCG+ GV VC
Sbjct: 277 ENPHTVIVYGDYYNALLWILRRAFTLGFDEASLQKSCCGIGGNYNFSLMKMCGVGGVSVC 336

Query: 301 ENPNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHCIV 339
            NP+E +SWDG+HLTQKAYKFMA+WLIHDIF +LHCIV
Sbjct: 337 SNPDERVSWDGIHLTQKAYKFMAYWLIHDIFPQLHCIV 374

BLAST of Lsi03G006730 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 4.4e-80
Identity = 148/331 (44.71%), Postives = 217/331 (65.56%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNL--IRENLNTPFSHL-PYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F +I   GDSI+DTGNL  + +  + P S   PYGETFF++PTGR S+G L+ID+ A   
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFL 93

Query: 62  GLPFVNPYLN-KDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHF 121
           G P V P+   ++A    GVNFAVAG+TAL    L ++ I S +TN SL+ QL       
Sbjct: 94  GFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESL 153

Query: 122 NSICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERV 181
            ++C +  +C + + NAL L+GEIGGNDYN+ALFQ K ++EV+++VP V+ +I SA+  +
Sbjct: 154 PNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITEL 213

Query: 182 ISYGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDEL-HCLKDLNSFASYHNDQIKEMIK 241
           +  G    +VPGNFPIG    YLT ++T++   YD L  CLK LN F+ Y+N Q++E + 
Sbjct: 214 VCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELN 273

Query: 242 ALKKENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLG 301
            L+K  PH  I+Y DYYNA L + +     GF    L  +CCGVGG+YNFN  + CG +G
Sbjct: 274 GLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PACCGVGGSYNFNFSRRCGSVG 333

Query: 302 VEVCENPNEHISWDGVHLTQKAYKFMAHWLI 328
           VE C++P++++++DG+H+T+ AY+ ++  L+
Sbjct: 334 VEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

BLAST of Lsi03G006730 vs. TAIR 10
Match: AT1G31550.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 292.0 bits (746), Expect = 6.3e-79
Identity = 152/331 (45.92%), Postives = 210/331 (63.44%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNL--IRENLNTPFSHL-PYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F++I   GDSI+DTGNL  + ++ N P S   PYGETFF++PTGR S+G L+ID+ A   
Sbjct: 34  FESIISFGDSIADTGNLLGLSDHNNLPMSAFPPYGETFFHHPTGRFSDGRLIIDFIAEFL 93

Query: 62  GLPFVNPYL-NKDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHF 121
           GLP+V PY  + +     GVNFAVA +TAL S  L +K    P  N SL  QL       
Sbjct: 94  GLPYVPPYFGSTNGNFEKGVNFAVASATALESSFLEEKGYHCP-HNFSLGVQLKIFKQSL 153

Query: 122 NSICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERV 181
            ++C    +C + + NAL L+GEIG NDYN+  FQ + + EVK++VP V+ +I SA+  +
Sbjct: 154 PNLCGLPSDCRDMIGNALILMGEIGANDYNFPFFQLRPLDEVKELVPLVISTISSAITEL 213

Query: 182 ISYGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDEL-HCLKDLNSFASYHNDQIKEMIK 241
           I  G    +VPG FP+GC   +LT  QT++   YD L  CLK LN F  YH++Q++E + 
Sbjct: 214 IGMGGRTFLVPGGFPLGCSVAFLTLHQTSNMEEYDPLTGCLKWLNKFGEYHSEQLQEELN 273

Query: 242 ALKKENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLG 301
            L+K NPH  I+Y DYYNA L + R     GF    L  +CCGVGG YNFNL + CG +G
Sbjct: 274 RLRKLNPHVNIIYADYYNASLRLGREPSKYGFINRHL-SACCGVGGPYNFNLSRSCGSVG 333

Query: 302 VEVCENPNEHISWDGVHLTQKAYKFMAHWLI 328
           VE C +P+++++WDG+H+T+ A+K MA  L+
Sbjct: 334 VEACSDPSKYVAWDGLHMTEAAHKSMADGLV 362

BLAST of Lsi03G006730 vs. TAIR 10
Match: AT1G28570.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 291.2 bits (744), Expect = 1.1e-78
Identity = 147/331 (44.41%), Postives = 210/331 (63.44%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNL--IRENLNTP-FSHLPYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F +I   GDSI+DTGNL  + +  N P  + LPYGETFF++PTGR SNG L+ID+ A   
Sbjct: 32  FKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHPTGRFSNGRLIIDFIAEFL 91

Query: 62  GLPFVNP-YLNKDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHF 121
           G P V P Y +++A    GVNFAV G+TAL    L ++ I  P TN SL  QL       
Sbjct: 92  GFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLEERGIHFPYTNVSLAVQLSSFKESL 151

Query: 122 NSICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERV 181
            ++C +  +C + + N+L L+GEIGGNDYNYA F GKNI+E+K++VP V+++I SA+  +
Sbjct: 152 PNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVIETISSAITEL 211

Query: 182 ISYGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYDEL-HCLKDLNSFASYHNDQIKEMIK 241
           I  G    +VPG FP+GC   YL+ +QT++   YD L  CLK LN F+ YH++Q++  + 
Sbjct: 212 IGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELN 271

Query: 242 ALKKENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLG 301
            L+K  PH  I+Y DYYN  L + +     GF    L  +CC +GG +NF L +  G   
Sbjct: 272 RLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRPL-PACCALGGPFNFTLGRKRGTQV 331

Query: 302 VEVCENPNEHISWDGVHLTQKAYKFMAHWLI 328
            E C++P++++SWDGVH+T+ AY+ MA  ++
Sbjct: 332 PECCDDPSKYVSWDGVHMTEAAYRLMAEGIL 361

BLAST of Lsi03G006730 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 291.2 bits (744), Expect = 1.1e-78
Identity = 154/334 (46.11%), Postives = 200/334 (59.88%), Query Frame = 0

Query: 3   DAIYQLGDSISDTGNLIRENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDAGLPF 62
           ++IYQ GDSISDTGNLIR                 N P                 A  P 
Sbjct: 28  NSIYQFGDSISDTGNLIR-----------------NGP-----------------ASSPT 87

Query: 63  VNPYLNKDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHFNSICY 122
             P   ++   N  VNF V+GSTAL S   S++ +  P TN+ L+ QL W   H  S C+
Sbjct: 88  PKPLPQRE--HNVFVNFGVSGSTALNSSFFSERNLHVPATNTPLSMQLAWFKGHLRSTCH 147

Query: 123 NQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERVISYGA 182
                ++ L ++LF+VGEIGGNDYNY  FQGK ++E++  +P VV +I +A   VI  GA
Sbjct: 148 G--SSSDCLKHSLFMVGEIGGNDYNYGFFQGKPMEEIRSYIPHVVGAITAAAREVIRAGA 207

Query: 183 TRVVVPGNFPIGCFPIYLTGFQTNDTTAYDELHCLKDLNSFASYHNDQIKEMIKALKKEN 242
             VVVPGNFP+GCFPIYLT F   DT  YD+  CL  LN FA  HN+Q++E I +L+KE 
Sbjct: 208 VNVVVPGNFPVGCFPIYLTSFPVKDTKDYDDNGCLTHLNEFAMDHNNQLQEAIASLRKEF 267

Query: 243 PHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLGVEVCEN 302
           P   IVYGDYYNAF ++LR      FD+    KSCCG GG YN++  +  G +GV VC+N
Sbjct: 268 PDVAIVYGDYYNAFQYVLRSE---RFDKSVALKSCCGTGGAYNYDGKRPYGAVGVPVCQN 320

Query: 303 PNEHISWDGVHLTQKAYKFMAHWLIHDIFLKLHC 337
           P++ ISWDGVHLTQKAY+FM+ +L + I  ++ C
Sbjct: 328 PHKFISWDGVHLTQKAYRFMSKFLNNQILSQIKC 320

BLAST of Lsi03G006730 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 289.7 bits (740), Expect = 3.1e-78
Identity = 144/332 (43.37%), Postives = 211/332 (63.55%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNLI---RENLNTPFSHLPYGETFFNNPTGRCSNGLLMIDYFALDA 61
           F +I   GDSI+DTGNL+     N     +  PYGETFF++PTGR  +G +++D+ A   
Sbjct: 29  FKSIISFGDSIADTGNLVGLSDRNQLPVTAFPPYGETFFHHPTGRSCDGRIIMDFIAEFV 88

Query: 62  GLPFVNPYL-NKDALTNHGVNFAVAGSTALPSELLSQKKISSPVTNSSLNHQLDWMFSHF 121
           GLP+V PY  +K+   + GVNFAVAG+TAL S  L ++ I  P TN SL  QL       
Sbjct: 89  GLPYVPPYFGSKNRNFDKGVNFAVAGATALKSSFLKKRGI-QPHTNVSLGVQLKSFKKSL 148

Query: 122 NSICYNQRECTEKLHNALFLVGEIGGNDYNYALFQGKNIQEVKDMVPDVVQSIKSAVERV 181
            ++C +  +C + + NAL L+GEIGGNDYN+  F  K ++EV+++VP V+ SI S +  +
Sbjct: 149 PNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITEL 208

Query: 182 ISYGATRVVVPGNFPIGCFPIYLTGFQTNDTTAYD-ELHCLKDLNSFASYHNDQIKEMIK 241
           I  G    +VPG FPIGC  +YLT ++T++   YD    CLK LN F  YH++++K  + 
Sbjct: 209 IGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELN 268

Query: 242 ALKKENPHTVIVYGDYYNAFLWILRRAFVLGFDEGSLQKSCCGVGGNYNFNLMKMCGLLG 301
            L+K  PH  I+Y DYYN+ L I +     GF E     +CCG+GG YNFN  + CG +G
Sbjct: 269 RLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPF-PACCGIGGPYNFNFTRKCGSVG 328

Query: 302 VEVCENPNEHISWDGVHLTQKAYKFMAHWLIH 329
           V+ C++P++++ WDGVH+T+ AYK++A  +++
Sbjct: 329 VKSCKDPSKYVGWDGVHMTEAAYKWIADGILN 358