Lsi03G006560 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G006560
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-type domain-containing protein
Location	chr03: 8300521 .. 8301750 (-)
RNA-Seq Expression	Lsi03G006560
Synteny	Lsi03G006560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGTAAGAAGAAATTATATATATATCCCTGCAACCGCTCAAGTTAATATATTATAGATTTAAATTATAACTTAATAAACAAAAAAAATTAAATATCTATATATATAATATAATAAATCAAAGAAAATTTTGTAATTTTGTATTAAAAGAAGTTAGAATGGTGAAATAATTAACGAACGCATTTGAAAAGATAGATTTTTTTTTTATCTAATATCTTTCATTCCTATTCCAATTACGAAAACATGATCCAAAGATAAAAGATATAATTGTCTTCAAAAAAAGATAAAAGATATAATTTCTTTCTCCAAATATATATATAAATTAATTAAGTAATGATATCTTGGTTTTCTAATAGAAGGGGATTTCCCATAGGCTTAAGGAAATCGGCAAGGTCTCTCTTATAAATGCCTCACATTCTATTATCAAATTTTCACAACTTTTCCCATTAATAACTCTCATCATTTAAATATTAATAATGGCGGCACAAATGTTTGAAATTTGTAGGAATGATCCTTCCTACATTGGTATGTCACTCACAATTAAAGACATATTCAAACTCGACAAAATTCATCAACATTTATTAAACAAATCGATCTGAATTTCAAAGTCCAACTCAAATGTAGTCTCTACCAAATATATGAAGAAGGCAATAGGAATACATCATCTTTCCTCGGTGAAGACATGTCTTCACAACATCCCTTTGCTACATACCAAATTCTTGTCTCCATCTTAAAACACGGCGACAAAACTTTGGAGCGACATCTATTGCAGCAGTTTCGGAGGTATAGAGACATCATCAATATCAAACTTGTGGCTAATGAAATAATAAAATACTGGGTTAGAAAAGTCGAAGAAGAAGAGGAGGATAATTTTAGGGTGTTTGGAAAGGTTTATCCTCTCGAAATTACGATAGAACTGTTGCTTTTTGAGAGAGTTGACAATGACACGATAGAAGTACCGTTAATGATGGTTCCAGCCAATAATAGCGCAATGGAGAGTATGTTAAAGAGAATGGAGATCGAAGAGATAATGAAATTAAGTGATGATGAAAGCATTAATTGCGTAATTTGTATGGAGGAAATTAGTAGAGAAGAGGAAGGAAAAAGTGGGACATTTGTGTTACAAATGCCTTGCTTGCATATGTTTCATGAAGAATGTATAAAGAAGTGGTCGAAGAGTAGTCATTATTGTCCAACTTGTAGATTTTCAATGCCTACAAATGATTGA

mRNA sequence

ATGGATTTAGAATGGGGATTTCCCATAGGCTTAAGGAAATCGGCAAGACATATTCAAACTCGACAAAATTCATCAACATTTATTAAACAAATCGATCTGAATTTCAAAGTCCAACTCAAATGTAGTCTCTACCAAATATATGAAGAAGGCAATAGGAATACATCATCTTTCCTCGGTGAAGACATGTCTTCACAACATCCCTTTGCTACATACCAAATTCTTGTCTCCATCTTAAAACACGGCGACAAAACTTTGGAGCGACATCTATTGCAGCAGTTTCGGAGGTATAGAGACATCATCAATATCAAACTTGTGGCTAATGAAATAATAAAATACTGGGTTAGAAAAGTCGAAGAAGAAGAGGAGGATAATTTTAGGGTGTTTGGAAAGGTTTATCCTCTCGAAATTACGATAGAACTGTTGCTTTTTGAGAGAGTTGACAATGACACGATAGAAGTACCGTTAATGATGGTTCCAGCCAATAATAGCGCAATGGAGAGTATGTTAAAGAGAATGGAGATCGAAGAGATAATGAAATTAAGTGATGATGAAAGCATTAATTGCGTAATTTGTATGGAGGAAATTAGTAGAGAAGAGGAAGGAAAAAGTGGGACATTTGTGTTACAAATGCCTTGCTTGCATATGTTTCATGAAGAATGTATAAAGAAGTGGTCGAAGAGTAGTCATTATTGTCCAACTTGTAGATTTTCAATGCCTACAAATGATTGA

Coding sequence (CDS)

ATGGATTTAGAATGGGGATTTCCCATAGGCTTAAGGAAATCGGCAAGACATATTCAAACTCGACAAAATTCATCAACATTTATTAAACAAATCGATCTGAATTTCAAAGTCCAACTCAAATGTAGTCTCTACCAAATATATGAAGAAGGCAATAGGAATACATCATCTTTCCTCGGTGAAGACATGTCTTCACAACATCCCTTTGCTACATACCAAATTCTTGTCTCCATCTTAAAACACGGCGACAAAACTTTGGAGCGACATCTATTGCAGCAGTTTCGGAGGTATAGAGACATCATCAATATCAAACTTGTGGCTAATGAAATAATAAAATACTGGGTTAGAAAAGTCGAAGAAGAAGAGGAGGATAATTTTAGGGTGTTTGGAAAGGTTTATCCTCTCGAAATTACGATAGAACTGTTGCTTTTTGAGAGAGTTGACAATGACACGATAGAAGTACCGTTAATGATGGTTCCAGCCAATAATAGCGCAATGGAGAGTATGTTAAAGAGAATGGAGATCGAAGAGATAATGAAATTAAGTGATGATGAAAGCATTAATTGCGTAATTTGTATGGAGGAAATTAGTAGAGAAGAGGAAGGAAAAAGTGGGACATTTGTGTTACAAATGCCTTGCTTGCATATGTTTCATGAAGAATGTATAAAGAAGTGGTCGAAGAGTAGTCATTATTGTCCAACTTGTAGATTTTCAATGCCTACAAATGATTGA

Protein sequence

MDLEWGFPIGLRKSARHIQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSILKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEEDNFRVFGKVYPLEITIELLLFERVDNDTIEVPLMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND

Homology

[Close]

BLAST of Lsi03G006560 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.3e-09
Identity = 29/84 (34.52%), Postives = 50/84 (59.52%), Query Frame = 0


Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHE 218
           PA+ SA++++      ++++K    E   C +CM+E       + G+ V QMPC H+FH+
Sbjct: 190 PASKSAIDALPTVKVTKDMLK---SEMNQCAVCMDEF------EDGSDVKQMPCKHVFHQ 249

Query: 219 ECIKKWSKSSHYCPTCRFSMPTND 243
           +C+  W +  + CP CRF +PT+D
Sbjct: 250 DCLLPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of Lsi03G006560 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.4e-08
Identity = 32/82 (39.02%), Postives = 46/82 (56.10%), Query Frame = 0


Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHE 218
           PA+ SA+ES L R+EI +    S+    NC +C E    E E +      +MPC H+FH+
Sbjct: 174 PASKSAIES-LPRVEISDCHIGSE---ANCAVCTEIFETETEAR------EMPCKHLFHD 233

Query: 219 ECIKKWSKSSHYCPTCRFSMPT 241
           +CI  W    + CP CRF +P+
Sbjct: 234 DCIVPWLSIRNSCPVCRFELPS 245

[Close]

BLAST of Lsi03G006560 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 5.3e-08
Identity = 31/83 (37.35%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHE 218
           PA+ SA+ES L R+EI +    ++    NC +C E      EG+      +MPC H+FH 
Sbjct: 190 PASKSAIES-LPRVEISDCHTKAE---ANCAVCTEVFEAGIEGR------EMPCKHIFHG 249

Query: 219 ECIKKWSKSSHYCPTCRFSMPTN 242
           +CI  W    + CP CRF +P++
Sbjct: 250 DCIVPWLSIRNSCPVCRFELPSD 262

[Close]

BLAST of Lsi03G006560 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 9.0e-08
Identity = 28/85 (32.94%), Postives = 48/85 (56.47%), Query Frame = 0


Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESIN-CVICMEEISREEEGKSGTFVLQMPCLHMFH 218
           PA+ SA+E+    + +  I K + +   N C +CM++       + GT   QMPC H++H
Sbjct: 198 PASKSAIEA----LPLVNITKSNLNSEFNQCAVCMDDF------EEGTEAKQMPCKHLYH 257

Query: 219 EECIKKWSKSSHYCPTCRFSMPTND 243
           ++C+  W +  + CP CR  +PT+D
Sbjct: 258 KDCLLPWLELHNSCPVCRHELPTDD 272

[Close]

BLAST of Lsi03G006560 vs. ExPASy Swiss-Prot
Match: Q9VE61 (E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster OX=7227 GN=CG7694 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.6e-07
Identity = 31/92 (33.70%), Postives = 46/92 (50.00%), Query Frame = 0


Query: 151 IEVPLMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQM 210
           IEVP         A +  +  + + EI+K  +   + C +C E     EEG+       +
Sbjct: 41  IEVP--------EASKRAILELPVHEIVKSDEGGDLECSVCKEP---AEEGQKYRI---L 100

Query: 211 PCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           PC H FHEECI  W K ++ CP CR+ + T+D
Sbjct: 101 PCKHEFHEECILLWLKKTNSCPLCRYELETDD 118

[Close]

BLAST of Lsi03G006560 vs. ExPASy TrEMBL
Match: A0A0A0K2A5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G068580 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 1.1e-72
Identity = 145/240 (60.42%), Postives = 181/240 (75.42%), Query Frame = 0


Query: 8   PIGLRKSARHIQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHP 67
           P+  R+    I+TR+NS TF KQIDLNF+VQLKC  +QI EE   NT++ + E + SQHP
Sbjct: 25  PVQRRRPQTQIRTRRNSPTFFKQIDLNFEVQLKCQHFQIREEETSNTTTLIDEHIVSQHP 84

Query: 68  FATYQILVSILKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEED---- 127
           FAT+++ +SIL+HGDKTL+  L ++F  YRDIINI+ + +EIIKYWV KVEEEEED    
Sbjct: 85  FATFRLPISILEHGDKTLKLLLFREFHMYRDIINIEHLVDEIIKYWVTKVEEEEEDQENS 144

Query: 128 -NFRVFGKVYPLEITIELLLFERVD-NDTIEVPLMMVPANNSAMESMLKRMEIEEIMKLS 187
            + RVF K+Y LEIT+ELL+F+ V   D     LMMVP ++SAMESMLKR+E EEIMKL 
Sbjct: 145 TSSRVFKKIYRLEITLELLIFQIVRVMDYQPQVLMMVPTSDSAMESMLKRVENEEIMKLG 204

Query: 188 DDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTN 242
           DD+ I+CVIC+EEI +EE+G  G  VLQMPCLH+FH ECI KW  +SHYCPTCRF MP N
Sbjct: 205 DDDCIHCVICLEEIGKEEKGSEGV-VLQMPCLHVFHGECINKWLNTSHYCPTCRFPMPIN 263

[Close]

BLAST of Lsi03G006560 vs. ExPASy TrEMBL
Match: A0A0A0K2A1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067550 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.8e-68
Identity = 134/231 (58.01%), Postives = 179/231 (77.49%), Query Frame = 0


Query: 18  IQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSI 77
           IQTR NS T +KQIDLNFKV++KC  +Q  EE     ++FL E + +  PF   Q+ VSI
Sbjct: 25  IQTRSNSPTSVKQIDLNFKVEVKCQHFQAGEEDTTFATTFLSEHIIAHQPFPALQLPVSI 84

Query: 78  LKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEEDNFRVFGKVYPLEIT 137
            KHGDKTL+R L Q+F+ +R IINI+LV ++IIK+WV+KVE+++E+N  VF ++YPLEIT
Sbjct: 85  FKHGDKTLKRLLFQKFQMFRAIINIELVTDQIIKHWVKKVEDDKENNSGVFEEIYPLEIT 144

Query: 138 IELLLFERVD-----NDTIEVP-LMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVIC 197
           IE+L+ + +D      +TI+ P + MVP ++SAM+SMLKR+E EEIMK+ D +SI+CVIC
Sbjct: 145 IEVLVLQMIDAVDQPQETIDQPQVRMVPTSDSAMKSMLKRVENEEIMKVGDYKSISCVIC 204

Query: 198 MEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           +E+IS+EE+G  G  VLQMPCLHMFHEECI+KW K+SHYCPTCRFSM  N+
Sbjct: 205 LEKISKEEKGSEGV-VLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMTINN 254

[Close]

BLAST of Lsi03G006560 vs. ExPASy TrEMBL
Match: A0A5D3C4K6 (Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001670 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 1.4e-67
Identity = 139/228 (60.96%), Postives = 179/228 (78.51%), Query Frame = 0


Query: 23  NSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSILKHGD 82
           +S T ++QI L FKVQLKC  +Q++E GN ++++ + E + SQH FAT+Q+ +SI KHGD
Sbjct: 40  SSPTLVEQICLKFKVQLKCQHFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHGD 99

Query: 83  KTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEE--EDNFRV-FGKVYPLEITIE 142
           KTL+R L ++F+ Y DIINI+L+ANEIIK+WVRKVEEEE  E+N  V F K+YPLEI I 
Sbjct: 100 KTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEEEEDQENNSNVLFKKIYPLEIMIR 159

Query: 143 LLLFERVDN-----DTIEVPLMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVICMEE 202
           LL+ + +         I+ PL MVP ++SA+ESMLKR+E EEIMK  DDESINCV+C+EE
Sbjct: 160 LLVSKMIRAVQPRLAMIDQPL-MVPTSDSAVESMLKRVENEEIMKSGDDESINCVVCLEE 219

Query: 203 ISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           IS+EE+G S T VLQMPCLHMFHEECI+KW K+SHYCPTCRFSMP ++
Sbjct: 220 ISKEEKG-SETTVLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]

BLAST of Lsi03G006560 vs. ExPASy TrEMBL
Match: E5GCQ3 (Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 1.4e-67
Identity = 139/228 (60.96%), Postives = 179/228 (78.51%), Query Frame = 0


Query: 23  NSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSILKHGD 82
           +S T ++QI L FKVQLKC  +Q++E GN ++++ + E + SQH FAT+Q+ +SI KHGD
Sbjct: 40  SSPTLVEQICLKFKVQLKCQHFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHGD 99

Query: 83  KTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEE--EDNFRV-FGKVYPLEITIE 142
           KTL+R L ++F+ Y DIINI+L+ANEIIK+WVRKVEEEE  E+N  V F K+YPLEI I 
Sbjct: 100 KTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEEEEDQENNSNVLFKKIYPLEIMIR 159

Query: 143 LLLFERVDN-----DTIEVPLMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVICMEE 202
           LL+ + +         I+ PL MVP ++SA+ESMLKR+E EEIMK  DDESINCV+C+EE
Sbjct: 160 LLVSKMIRAVQPRLAMIDQPL-MVPTSDSAVESMLKRVENEEIMKSGDDESINCVVCLEE 219

Query: 203 ISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           IS+EE+G S T VLQMPCLHMFHEECI+KW K+SHYCPTCRFSMP ++
Sbjct: 220 ISKEEKG-SETTVLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]

BLAST of Lsi03G006560 vs. ExPASy TrEMBL
Match: A0A5A7SPE2 (Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G001670 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 2.0e-66
Identity = 133/241 (55.19%), Postives = 178/241 (73.86%), Query Frame = 0


Query: 11  LRKSARHIQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFAT 70
           + ++ + I+TRQNSS +++ IDLNFKVQLKCS + +YE+G   T+SFL E  S  HP AT
Sbjct: 28  MNRTRKRIRTRQNSSKYVQHIDLNFKVQLKCSHFLVYEDGITTTTSFLNEHTSRPHPLAT 87

Query: 71  YQILVSILKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEE-----EEEDN- 130
           Y++ +SI KHGDK LE++L  +F  YR+ INI LVA+ +I++WV++VEE     +EE+N 
Sbjct: 88  YKLPISIFKHGDKALEQYLFHKFHVYREFINITLVADRLIQHWVKRVEEDDHEYQEENNN 147

Query: 131 --FRVFGKVYPLEITIELLLFERVDNDTIEVPLM-MVPANNSAMESMLKRMEIEEIMKLS 190
             F VF K+YPLEIT+E+ + +R      E P+M MVP ++SA+ESML+R+E +      
Sbjct: 148 HDFNVFQKIYPLEITLEMQIIQRY---VEESPIMIMVPTSDSAIESMLRRVEND-----W 207

Query: 191 DDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTN 243
           DDE INCVIC+EEIS +EE ++   VLQMPCLHMFH ECI KW K+SHYCPTCRFSMPTN
Sbjct: 208 DDEGINCVICLEEISSKEEEETEKSVLQMPCLHMFHGECINKWLKTSHYCPTCRFSMPTN 260

[Close]

BLAST of Lsi03G006560 vs. NCBI nr
Match: KAE8645828.1 (hypothetical protein Csa_017274 [Cucumis sativus])

HSP 1 Score: 271.6 bits (693), Expect = 6.8e-69
Identity = 135/231 (58.44%), Postives = 180/231 (77.92%), Query Frame = 0


Query: 18  IQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSI 77
           IQTR NS T +KQIDLNFKV++KC  +Q  EE     ++FL E + +  PF   Q+ VSI
Sbjct: 291 IQTRSNSPTSVKQIDLNFKVEVKCQHFQAGEEDTTFATTFLSEHIIAHQPFPALQLPVSI 350

Query: 78  LKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEEDNFRVFGKVYPLEIT 137
            KHGDKTL+R L Q+F+ +R IINI+LV ++IIK+WV+KVE+++E+N  VF ++YPLEIT
Sbjct: 351 FKHGDKTLKRLLFQKFQMFRAIINIELVTDQIIKHWVKKVEDDKENNSGVFEEIYPLEIT 410

Query: 138 IELLLFERVD-----NDTIEVP-LMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVIC 197
           IE+L+ + +D      +TI+ P + MVP ++SAM+SMLKR+E EEIMK+ D +SI+CVIC
Sbjct: 411 IEVLVLQMIDAVDQPQETIDQPQVRMVPTSDSAMKSMLKRVENEEIMKVGDYKSISCVIC 470

Query: 198 MEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           +E+IS+EE+G  G  VLQMPCLHMFHEECI+KW K+SHYCPTCRFSMP N+
Sbjct: 471 LEKISKEEKGSEGV-VLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMPINN 520

[Close]

BLAST of Lsi03G006560 vs. NCBI nr
Match: ADN34252.1 (hypothetical zinc finger protein [Cucumis melo subsp. melo] >KAA0031412.1 putative zinc finger protein [Cucumis melo var. makuwa] >TYK06863.1 putative zinc finger protein [Cucumis melo var. makuwa])

HSP 1 Score: 266.2 bits (679), Expect = 2.8e-67
Identity = 139/228 (60.96%), Postives = 179/228 (78.51%), Query Frame = 0


Query: 23  NSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSILKHGD 82
           +S T ++QI L FKVQLKC  +Q++E GN ++++ + E + SQH FAT+Q+ +SI KHGD
Sbjct: 40  SSPTLVEQICLKFKVQLKCQHFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHGD 99

Query: 83  KTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEE--EDNFRV-FGKVYPLEITIE 142
           KTL+R L ++F+ Y DIINI+L+ANEIIK+WVRKVEEEE  E+N  V F K+YPLEI I 
Sbjct: 100 KTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEEEEDQENNSNVLFKKIYPLEIMIR 159

Query: 143 LLLFERVDN-----DTIEVPLMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVICMEE 202
           LL+ + +         I+ PL MVP ++SA+ESMLKR+E EEIMK  DDESINCV+C+EE
Sbjct: 160 LLVSKMIRAVQPRLAMIDQPL-MVPTSDSAVESMLKRVENEEIMKSGDDESINCVVCLEE 219

Query: 203 ISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           IS+EE+G S T VLQMPCLHMFHEECI+KW K+SHYCPTCRFSMP ++
Sbjct: 220 ISKEEKG-SETTVLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]

BLAST of Lsi03G006560 vs. NCBI nr
Match: KAA0031411.1 (putative zinc finger protein [Cucumis melo var. makuwa])

HSP 1 Score: 262.3 bits (669), Expect = 4.1e-66
Identity = 133/241 (55.19%), Postives = 178/241 (73.86%), Query Frame = 0


Query: 11  LRKSARHIQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFAT 70
           + ++ + I+TRQNSS +++ IDLNFKVQLKCS + +YE+G   T+SFL E  S  HP AT
Sbjct: 28  MNRTRKRIRTRQNSSKYVQHIDLNFKVQLKCSHFLVYEDGITTTTSFLNEHTSRPHPLAT 87

Query: 71  YQILVSILKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEE-----EEEDN- 130
           Y++ +SI KHGDK LE++L  +F  YR+ INI LVA+ +I++WV++VEE     +EE+N 
Sbjct: 88  YKLPISIFKHGDKALEQYLFHKFHVYREFINITLVADRLIQHWVKRVEEDDHEYQEENNN 147

Query: 131 --FRVFGKVYPLEITIELLLFERVDNDTIEVPLM-MVPANNSAMESMLKRMEIEEIMKLS 190
             F VF K+YPLEIT+E+ + +R      E P+M MVP ++SA+ESML+R+E +      
Sbjct: 148 HDFNVFQKIYPLEITLEMQIIQRY---VEESPIMIMVPTSDSAIESMLRRVEND-----W 207

Query: 191 DDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTN 243
           DDE INCVIC+EEIS +EE ++   VLQMPCLHMFH ECI KW K+SHYCPTCRFSMPTN
Sbjct: 208 DDEGINCVICLEEISSKEEEETEKSVLQMPCLHMFHGECINKWLKTSHYCPTCRFSMPTN 260

[Close]

BLAST of Lsi03G006560 vs. NCBI nr
Match: TYK06862.1 (putative zinc finger protein [Cucumis melo var. makuwa])

HSP 1 Score: 261.5 bits (667), Expect = 7.0e-66
Identity = 133/241 (55.19%), Postives = 177/241 (73.44%), Query Frame = 0


Query: 11  LRKSARHIQTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFAT 70
           + ++ + I+TRQNSS +++ IDLNFKVQLKCS + +YE+G   T+SFL E  S  HP AT
Sbjct: 28  MNRTRKRIRTRQNSSKYVQHIDLNFKVQLKCSHFLVYEDGITTTTSFLNEHTSRPHPLAT 87

Query: 71  YQILVSILKHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEE-----EEEDN- 130
           Y++ +SI KHGDK LE++L  +F  YR+ IN  LVA+ +I++WV++VEE     +EE+N 
Sbjct: 88  YKLPISIFKHGDKALEQYLFHKFHVYREFINTTLVADRLIQHWVKRVEEDDHEHQEENNN 147

Query: 131 --FRVFGKVYPLEITIELLLFERVDNDTIEVPLM-MVPANNSAMESMLKRMEIEEIMKLS 190
             F VF K+YPLEIT+E+ + +R      E P+M MVP ++SA+ESML+R+E +      
Sbjct: 148 HDFNVFQKIYPLEITLEMRIIQRY---VEESPIMIMVPTSDSAIESMLRRVEND-----W 207

Query: 191 DDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTN 243
           DDE INCVIC+EEIS +EE +S   VLQMPCLHMFH ECI KW K+SHYCPTCRFSMPTN
Sbjct: 208 DDEGINCVICLEEISSKEEEESEKSVLQMPCLHMFHGECINKWLKTSHYCPTCRFSMPTN 260

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BLAST of Lsi03G006560 vs. NCBI nr
Match: KGN43799.1 (hypothetical protein Csa_017222 [Cucumis sativus])

HSP 1 Score: 257.7 bits (657), Expect = 1.0e-64
Identity = 129/230 (56.09%), Postives = 175/230 (76.09%), Query Frame = 0


Query: 19  QTRQNSSTFIKQIDLNFKVQLKCSLYQIYEEGNRNTSSFLGEDMSSQHPFATYQILVSIL 78
           QT  NS TF+KQI+L  KV++KC  +Q  EEG+   S+ + E + S HPF T+Q+ VS+ 
Sbjct: 36  QTPHNSPTFVKQINLKLKVEVKCQHFQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVF 95

Query: 79  KHGDKTLERHLLQQFRRYRDIINIKLVANEIIKYWVRKVEEEEEDNFRVFGKVYPLEITI 138
           +HG++TL+R L QQF+ +R IINI+LVA+EII++WV+K E+ E ++  +  ++YPLEITI
Sbjct: 96  RHGNRTLKRLLFQQFQIFRGIINIQLVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITI 155

Query: 139 ELLLFERV-----DNDTIEVP-LMMVPANNSAMESMLKRMEIEEIMKLSDDESINCVICM 198
           ELLL   +       +TI+ P + MVPA+ SAMESMLKR+E EEI+K+ +D+SINCVIC+
Sbjct: 156 ELLLLRMIHAIDQPEETIDQPQVTMVPASESAMESMLKRVEKEEIVKVGEDKSINCVICL 215

Query: 199 EEISREEEGKSGTFVLQMPCLHMFHEECIKKWSKSSHYCPTCRFSMPTND 243
           EEIS++ +G  G  VLQMPCLHMFHEECI+ W K+SHYCPTCRFSMP N+
Sbjct: 216 EEISKKMKGSEGV-VLQMPCLHMFHEECIRTWLKTSHYCPTCRFSMPINN 264

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BLAST of Lsi03G006560 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 9.0e-11
Identity = 29/84 (34.52%), Postives = 50/84 (59.52%), Query Frame = 0


Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHE 218
           PA+ SA++++      ++++K    E   C +CM+E       + G+ V QMPC H+FH+
Sbjct: 190 PASKSAIDALPTVKVTKDMLK---SEMNQCAVCMDEF------EDGSDVKQMPCKHVFHQ 249

Query: 219 ECIKKWSKSSHYCPTCRFSMPTND 243
           +C+  W +  + CP CRF +PT+D
Sbjct: 250 DCLLPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of Lsi03G006560 vs. TAIR 10
Match: AT1G18760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 62.8 bits (151), Expect = 4.4e-10
Identity = 29/84 (34.52%), Postives = 45/84 (53.57%), Query Frame = 0


Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHE 218
           PAN   + S+ ++     I K +   +  C IC+EE +       GT V+ +PC H F +
Sbjct: 151 PANKLVVNSLARK-----IYKKTTSSTERCTICLEEFN------DGTKVMTLPCGHEFDD 210

Query: 219 ECIKKWSKSSHYCPTCRFSMPTND 243
           EC+  W +++H CP CRF +P  D
Sbjct: 211 ECVLTWFETNHDCPLCRFKLPCED 223

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BLAST of Lsi03G006560 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 61.6 bits (148), Expect = 9.9e-10
Identity = 32/82 (39.02%), Postives = 46/82 (56.10%), Query Frame = 0


Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHE 218
           PA+ SA+ES L R+EI +    S+    NC +C E    E E +      +MPC H+FH+
Sbjct: 174 PASKSAIES-LPRVEISDCHIGSE---ANCAVCTEIFETETEAR------EMPCKHLFHD 233

Query: 219 ECIKKWSKSSHYCPTCRFSMPT 241
           +CI  W    + CP CRF +P+
Sbjct: 234 DCIVPWLSIRNSCPVCRFELPS 245

[Close]

BLAST of Lsi03G006560 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 59.7 bits (143), Expect = 3.8e-09
Identity = 31/83 (37.35%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 159 PANNSAMESMLKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHE 218
           PA+ SA+ES L R+EI +    ++    NC +C E      EG+      +MPC H+FH 
Sbjct: 190 PASKSAIES-LPRVEISDCHTKAE---ANCAVCTEVFEAGIEGR------EMPCKHIFHG 249

Query: 219 ECIKKWSKSSHYCPTCRFSMPTN 242
           +CI  W    + CP CRF +P++
Sbjct: 250 DCIVPWLSIRNSCPVCRFELPSD 262

[Close]

BLAST of Lsi03G006560 vs. TAIR 10
Match: AT5G60820.1 (RING/U-box superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 4.9e-09
Identity = 40/137 (29.20%), Postives = 73/137 (53.28%), Query Frame = 0


Query: 106 ANEIIKYWVRKVEEEEEDNFRVFGKVYPLEITIELLLFERVDNDTIEVPLMMVPANNSAM 165
           A   ++ ++  ++ EEED      + Y L  T   +LFE   +  I  P    PA+ S +
Sbjct: 300 AESNLELYIGDIDHEEEDY-----EDY-LHTTEYEMLFEAEISSGIGKP----PASKSFI 359

Query: 166 ESM-LKRMEIEEIMKLSDDESINCVICMEEISREEEGKSGTFVLQMPCLHMFHEECIKKW 225
           +++ +  +  E++M+ +DD+++ C +C EE+   +E      V ++PC H +H ECI  W
Sbjct: 360 KNLKVSPLSNEDVME-NDDDAVCCAVCKEEMIVGKE------VAELPCRHKYHSECIVPW 419

Query: 226 SKSSHYCPTCRFSMPTN 242
               + CP CRF +P++
Sbjct: 420 LGIRNTCPVCRFELPSD 419

The following BLAST results are available for this feature:

BLAST of Lsi03G006560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	1.3e-09	34.52	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q940T5	1.4e-08	39.02	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q9SNB6	5.3e-08	37.35	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
P0CH30	9.0e-08	32.94	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q9VE61	2.6e-07	33.70	E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster OX=7227 GN...	[more]

BLAST of Lsi03G006560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K2A5	1.1e-72	60.42	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G068580 P...	[more]
A0A0A0K2A1	2.8e-68	58.01	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067550 P...	[more]
A0A5D3C4K6	1.4e-67	60.96	Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
E5GCQ3	1.4e-67	60.96	Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A5A7SPE2	2.0e-66	55.19	Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]

BLAST of Lsi03G006560 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8645828.1	6.8e-69	58.44	hypothetical protein Csa_017274 [Cucumis sativus]	[more]
ADN34252.1	2.8e-67	60.96	hypothetical zinc finger protein [Cucumis melo subsp. melo] >KAA0031412.1 putati...	[more]
KAA0031411.1	4.1e-66	55.19	putative zinc finger protein [Cucumis melo var. makuwa]	[more]
TYK06862.1	7.0e-66	55.19	putative zinc finger protein [Cucumis melo var. makuwa]	[more]
KGN43799.1	1.0e-64	56.09	hypothetical protein Csa_017222 [Cucumis sativus]	[more]

BLAST of Lsi03G006560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	9.0e-11	34.52	RING/U-box superfamily protein	[more]
AT1G18760.1	4.4e-10	34.52	Zinc finger, C3HC4 type (RING finger) family protein	[more]
AT5G59550.1	9.9e-10	39.02	zinc finger (C3HC4-type RING finger) family protein	[more]
AT3G46620.1	3.8e-09	37.35	zinc finger (C3HC4-type RING finger) family protein	[more]
AT5G60820.1	4.9e-09	29.20	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 188..234 e-value: 1.8E-8 score: 44.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 187..235 e-value: 1.4E-11 score: 44.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 188..235 score: 13.094151
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 152..241 e-value: 1.2E-17 score: 65.4
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 37..240
None	No IPR available	PANTHER	PTHR15710:SF63	RING/U-BOX SUPERFAMILY PROTEIN	coord: 37..240
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 170..240

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G006560.1	Lsi03G006560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity