Lsi03G006480 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G006480
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	subtilisin-like protease SBT3.4
Location	chr03: 8183360 .. 8184730 (+)
RNA-Seq Expression	Lsi03G006480
Synteny	Lsi03G006480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAACAAAAGCAATTCTTCTTAATTTTGTACAAAATTGTGACTCCATCTCTATTCCTAATTCCTATAGTGTTGAAGTTGAAAGAATGGCAAATACAAAGATGAATTTTTCCTCATTTACCTTGTATCTTCTTTTATTGTTGACAATTTCTCTGAATTTGCTTCTTAGCTCGACGACAACATCAGTAAGATCCACCACCTCCGAGATTGGTTTACTATCGTCATTGCATATCGTTTACACTTCTGAGAGGCCTAGCAATGAGAAACCTGAGGCCTTTAACCTCCAAATCCTAACTTCTGTTCTCGGCAGGTATGTTTTTCTTGAAAAATTATTCAAAGAGACTGCCACATTATTTTTAGACGAAAAATTATTCAATATGGACATCACAAAGTGGTGTGCAATTTTACACCATTGGATGTGGATTCTAAAATTTGCATGCTCATAATAACCACATGTTCTTTTACCGTTCTCTCTCATACATCCAACACTACAAAATTGTCGGCCGTATTATGGAATATTATCGTTGCTCGCAATGGTTAATTGTTAGGAACATCCACATTGAGATATGTTCATTGAATAATTTTTCGTTTTCTAAATACGTCTCGTGTGTGTCGTCTTTTCTCTGACCAAATGATGGGTTAATTGAAGGTGAATTTTTGTGTCAATTATTTGTTGGTTTTATTCCAGTAAAGAAGCTGCTAAGAAAGCTCTTGTGTATAGTTTTAATAATTCTATGACTGGCTTTGCTGCTAATCTGACTCCTGATCAGGTTAAGAAAATTTCAGGTAACTATTGTTCTAAATATTATGTTTTTTTTTTTTAATAATAACTTAGGATGTGATTAGATCTTCACAACTTTTTATTTTGGGATTTTGGCAGAACACCCCGGAGTTGTACATGTTGTCCAAAGCGTTAATTATAATTTGCAGACAGAAGGAAAAAGGGATAGTAAAAATGTATAGAAGAGTTTCAATATCTTTCTCTTCAAATATGGGTTTGTTGCTTAAATAAAAGGCGCAATTGCAAGTATAGCAATCATGCCCAAAATATTAGCAAATATAATACAATGCAAAAGAATTTACAAATATAACAAAATTTAGATCTAGCTCTTAGAGTTTATCGGTGATAAACTATATCGGTGATAGGACGAGTCTATTAGCGATAGAGTCTATCACTAATAGGAATTTATCATCGAATGAATCTATAATTGATAGATTTTGCTATATTTGTAATTCCTTAAAAATGTTGTTATACATTTAATTGTTAGTCCTAAATTGATACCCATTGTAATAACCGTGTTATTCATTGCAATTACCCTAAATAAAATGTATTTGGTGTTAAACCATGGTTTAAGGAGTTTTTATATCCCTT

mRNA sequence

CAAAACAAAAGCAATTCTTCTTAATTTTGTACAAAATTGTGACTCCATCTCTATTCCTAATTCCTATAGTGTTGAAGTTGAAAGAATGGCAAATACAAAGATGAATTTTTCCTCATTTACCTTGTATCTTCTTTTATTGTTGACAATTTCTCTGAATTTGCTTCTTAGCTCGACGACAACATCAGTAAGATCCACCACCTCCGAGATTGGTTTACTATCGTCATTGCATATCGTTTACACTTCTGAGAGGCCTAGCAATGAGAAACCTGAGGCCTTTAACCTCCAAATCCTAACTTCTGTTCTCGGCAGTAAAGAAGCTGCTAAGAAAGCTCTTGTGTATAGTTTTAATAATTCTATGACTGGCTTTGCTGCTAATCTGACTCCTGATCAGGTTAAGAAAATTTCAGAACACCCCGGAGTTGTACATGTTGTCCAAAGCGTTAATTATAATTTGCAGACAGAAGGAAAAAGGGATAGTAAAAATGTATAGAAGAGTTTCAATATCTTTCTCTTCAAATATGGGTTTGTTGCTTAAATAAAAGGCGCAATTGCAAGTATAGCAATCATGCCCAAAATATTAGCAAATATAATACAATGCAAAAGAATTTACAAATATAACAAAATTTAGATCTAGCTCTTAGAGTTTATCGGTGATAAACTATATCGGTGATAGGACGAGTCTATTAGCGATAGAGTCTATCACTAATAGGAATTTATCATCGAATGAATCTATAATTGATAGATTTTGCTATATTTGTAATTCCTTAAAAATGTTGTTATACATTTAATTGTTAGTCCTAAATTGATACCCATTGTAATAACCGTGTTATTCATTGCAATTACCCTAAATAAAATGTATTTGGTGTTAAACCATGGTTTAAGGAGTTTTTATATCCCTT

Coding sequence (CDS)

ATGGCAAATACAAAGATGAATTTTTCCTCATTTACCTTGTATCTTCTTTTATTGTTGACAATTTCTCTGAATTTGCTTCTTAGCTCGACGACAACATCAGTAAGATCCACCACCTCCGAGATTGGTTTACTATCGTCATTGCATATCGTTTACACTTCTGAGAGGCCTAGCAATGAGAAACCTGAGGCCTTTAACCTCCAAATCCTAACTTCTGTTCTCGGCAGTAAAGAAGCTGCTAAGAAAGCTCTTGTGTATAGTTTTAATAATTCTATGACTGGCTTTGCTGCTAATCTGACTCCTGATCAGGTTAAGAAAATTTCAGAACACCCCGGAGTTGTACATGTTGTCCAAAGCGTTAATTATAATTTGCAGACAGAAGGAAAAAGGGATAGTAAAAATGTATAG

Protein sequence

MANTKMNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQTEGKRDSKNV

Homology

[Close]

BLAST of Lsi03G006480 vs. ExPASy Swiss-Prot
Match: Q1PDX5 (Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 7.5e-12
Identity = 38/84 (45.24%), Postives = 58/84 (69.05%), Query Frame = 0


Query: 45  SSLHIVYTSERPSNEKPE---AFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPD 104
           + +HIVY  E+  N+ PE   + +L++L S+LGSK+ A +++V+S+ N  +GFAA+LT  
Sbjct: 35  TKVHIVYLGEKEHND-PELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDS 94

Query: 105 QVKKISEHPGVVHVVQSVNYNLQT 126
           Q ++ISEHP VV V  +  Y LQT
Sbjct: 95  QAEQISEHPDVVQVTPNTFYELQT 117

[Close]

BLAST of Lsi03G006480 vs. ExPASy Swiss-Prot
Match: Q8GUK4 (Subtilisin-like protease SBT3.13 OS=Arabidopsis thaliana OX=3702 GN=SBT3.13 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 4.1e-10
Identity = 51/123 (41.46%), Postives = 74/123 (60.16%), Query Frame = 0


Query: 6   MNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPE--- 65
           MN S  +  L+LLL I+L L L +T     +    +   S ++IVY  ER  ++ PE   
Sbjct: 1   MNNSLQSSKLVLLLAIALVLFL-NTELDFLTAAGALDSDSKVYIVYLGER-EHDDPELVT 60

Query: 66  AFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYN 125
           A + Q+L S+L SKE A+ +L+YS+ +  +GFAA LT  Q KKISEHP V+HV+ +    
Sbjct: 61  ASHHQMLESLLQSKEDAQNSLIYSYQHGFSGFAALLTSSQAKKISEHPEVIHVIPNRIRK 120

[Close]

BLAST of Lsi03G006480 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.6e-09
Identity = 47/123 (38.21%), Postives = 71/123 (57.72%), Query Frame = 0


Query: 6   MNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPE--- 65
           MN+ + ++Y++L L I LN+         RS  +E      +HIVY  E+  ++ PE   
Sbjct: 2   MNYRT-SIYVVLSLVIFLNV--------QRSFVAESSAKRKVHIVYLGEK-QHDDPEFVT 61

Query: 66  AFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYN 125
             + ++L S+LGSKE A  ++VYS+ +  +GFAA LT  Q KKI++ P VVHV+    Y 
Sbjct: 62  ESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYK 114

[Close]

BLAST of Lsi03G006480 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.0e-09
Identity = 42/107 (39.25%), Postives = 64/107 (59.81%), Query Frame = 0


Query: 22  SLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPE---AFNLQILTSVLGSKEA 81
           S+ L+L S  T + +T +     S +HIVY  E+  ++ PE     + Q+L S+LGSK+ 
Sbjct: 7   SILLVLLSLITVLNATRARSETESKVHIVYLGEKKHHD-PEFVTESHHQMLASLLGSKKD 66

Query: 82  AKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQT 126
           A  ++VYS+ +  +GFAA LT  Q KKI++ P VVHV+    + L T
Sbjct: 67  ADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELAT 112

[Close]

BLAST of Lsi03G006480 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 4.5e-09
Identity = 39/111 (35.14%), Postives = 65/111 (58.56%), Query Frame = 0


Query: 17  LLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKP--EAFNLQILTSVLG 76
           +L+ +SL ++L+    S +         S +HIVY  E+  ++       + Q+L+S+LG
Sbjct: 8   VLVVLSLIIVLNVARASAK---------SKVHIVYLGEKQHDDPKFVTESHHQMLSSLLG 67

Query: 77  SKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQT 126
           SK+ A +++VYS+ +  +GFAA LT  Q KKI++ P V+HV+    Y L T
Sbjct: 68  SKDDAHESMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYELAT 109

[Close]

BLAST of Lsi03G006480 vs. ExPASy TrEMBL
Match: A0A5D3C9P1 (Subtilisin-like protease SBT3.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G001980 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 7.7e-36
Identity = 90/131 (68.70%), Postives = 105/131 (80.15%), Query Frame = 0


Query: 1   MANTKMNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEK 60
           M NTKMNFSS  LYL+LL T+SLN LL+    S +S  S+  + SSLHIVYT++RPSNEK
Sbjct: 1   MTNTKMNFSSSPLYLVLLFTVSLNSLLAWGMISEKS-MSKFAVPSSLHIVYTAKRPSNEK 60

Query: 61  PEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVN 120
           PEAF +QIL SVLGS EAA+KALVYSF NSM GFAANLTP+QVKKIS  PGV+HV +SV 
Sbjct: 61  PEAFYIQILASVLGSNEAARKALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVT 120

Query: 121 YNLQTEGKRDS 132
           YNLQT GK ++
Sbjct: 121 YNLQTGGKENN 130

[Close]

BLAST of Lsi03G006480 vs. ExPASy TrEMBL
Match: A0A1S3CFT4 (subtilisin-like protease SBT3.4 OS=Cucumis melo OX=3656 GN=LOC103500439 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 7.7e-36
Identity = 90/131 (68.70%), Postives = 105/131 (80.15%), Query Frame = 0


Query: 1   MANTKMNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEK 60
           M NTKMNFSS  LYL+LL T+SLN LL+    S +S  S+  + SSLHIVYT++RPSNEK
Sbjct: 1   MTNTKMNFSSSPLYLVLLFTVSLNSLLAWGMISEKS-MSKFAVPSSLHIVYTAKRPSNEK 60

Query: 61  PEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVN 120
           PEAF +QIL SVLGS EAA+KALVYSF NSM GFAANLTP+QVKKIS  PGV+HV +SV 
Sbjct: 61  PEAFYIQILASVLGSNEAARKALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVT 120

Query: 121 YNLQTEGKRDS 132
           YNLQT GK ++
Sbjct: 121 YNLQTGGKENN 130

[Close]

BLAST of Lsi03G006480 vs. ExPASy TrEMBL
Match: A0A0A0K630 (Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G274680 PE=4 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 1.0e-32
Identity = 85/131 (64.89%), Postives = 104/131 (79.39%), Query Frame = 0


Query: 1   MANTKMNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEK 60
           M NTKMNFSSFTL L+LL TISLN  L S   S +   S+  + SS+++VYT++RPSNEK
Sbjct: 1   MTNTKMNFSSFTLSLVLLFTISLNSHLISGMISDK-PVSKFVVPSSIYMVYTAKRPSNEK 60

Query: 61  PEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVN 120
           PE F +QIL SV+ S EAAKKALVYSF +S+ GFAANLTP+QVKKI   PGV+HV +SVN
Sbjct: 61  PETFYIQILASVIDSNEAAKKALVYSFKSSINGFAANLTPNQVKKILAQPGVLHVARSVN 120

Query: 121 YNLQTEGKRDS 132
           YNLQTEGK+++
Sbjct: 121 YNLQTEGKKNN 130

[Close]

BLAST of Lsi03G006480 vs. ExPASy TrEMBL
Match: E5GCJ1 (Peptidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 9.1e-29
Identity = 75/113 (66.37%), Postives = 91/113 (80.53%), Query Frame = 0


Query: 19  LTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAFNLQILTSVLGSKEA 78
           +++SLN LL+    S +S  S+  + SSLHIVYT++RPSNEKPEAF +QIL SVLGS EA
Sbjct: 19  VSVSLNSLLAWGMISEKS-MSKFAVPSSLHIVYTAKRPSNEKPEAFYIQILASVLGSNEA 78

Query: 79  AKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQTEGKRDS 132
           A+KALVYSF NSM GFAANLTP+QVKKIS  PGV+HV +SV YNLQT GK ++
Sbjct: 79  ARKALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVTYNLQTGGKENN 130

[Close]

BLAST of Lsi03G006480 vs. ExPASy TrEMBL
Match: A0A6J1CPA2 (subtilisin-like protease SBT3.11 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013481 PE=4 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 7.0e-21
Identity = 65/123 (52.85%), Postives = 89/123 (72.36%), Query Frame = 0


Query: 5   KMNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAF 64
           K  FSSF L L LL +ISLN LLSS   S +  +       ++HIVYT E+P +E+PEA+
Sbjct: 3   KTTFSSFLLCLALLFSISLNFLLSSAAMSGQYAS------PAVHIVYT-EKPRDEEPEAY 62

Query: 65  NLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQ 124
           +++ L SVLGS+EAAK+ALVYS+ N+ +GF+A LTPDQV ++S+ PGV+HVVQS  + L 
Sbjct: 63  HIRTLVSVLGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLH 118

Query: 125 TEG 128
           + G
Sbjct: 123 SGG 118

[Close]

BLAST of Lsi03G006480 vs. NCBI nr
Match: XP_038896488.1 (subtilisin-like protease SBT3.13 [Benincasa hispida])

HSP 1 Score: 169.5 bits (428), Expect = 2.0e-38
Identity = 96/131 (73.28%), Postives = 109/131 (83.21%), Query Frame = 0


Query: 1   MANTKMNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEK 60
           MANTKMNFSSFTLYL+LL TISLN LL S   S +S  SEI +LSSL+IVYT+ERPSNEK
Sbjct: 1   MANTKMNFSSFTLYLVLLFTISLNSLLISAIISDKS-ASEIDVLSSLYIVYTTERPSNEK 60

Query: 61  PEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVN 120
           PEAF +QIL SVLGS+EAAKKALVYSF +SM GFAANLTPDQVKKIS  PGV++ VQ VN
Sbjct: 61  PEAFYIQILASVLGSEEAAKKALVYSFKSSMNGFAANLTPDQVKKISAQPGVLNFVQGVN 120

Query: 121 YNLQTEGKRDS 132
           Y L+  GK ++
Sbjct: 121 YELKATGKENN 130

[Close]

BLAST of Lsi03G006480 vs. NCBI nr
Match: XP_008461963.1 (PREDICTED: subtilisin-like protease SBT3.4 [Cucumis melo] >KAA0048207.1 subtilisin-like protease SBT3.4 [Cucumis melo var. makuwa] >TYK08050.1 subtilisin-like protease SBT3.4 [Cucumis melo var. makuwa])

HSP 1 Score: 159.8 bits (403), Expect = 1.6e-35
Identity = 90/131 (68.70%), Postives = 105/131 (80.15%), Query Frame = 0


Query: 1   MANTKMNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEK 60
           M NTKMNFSS  LYL+LL T+SLN LL+    S +S  S+  + SSLHIVYT++RPSNEK
Sbjct: 1   MTNTKMNFSSSPLYLVLLFTVSLNSLLAWGMISEKS-MSKFAVPSSLHIVYTAKRPSNEK 60

Query: 61  PEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVN 120
           PEAF +QIL SVLGS EAA+KALVYSF NSM GFAANLTP+QVKKIS  PGV+HV +SV 
Sbjct: 61  PEAFYIQILASVLGSNEAARKALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVT 120

Query: 121 YNLQTEGKRDS 132
           YNLQT GK ++
Sbjct: 121 YNLQTGGKENN 130

[Close]

BLAST of Lsi03G006480 vs. NCBI nr
Match: XP_038896487.1 (subtilisin-like protease SBT3.13 [Benincasa hispida])

HSP 1 Score: 153.7 bits (387), Expect = 1.1e-33
Identity = 87/126 (69.05%), Postives = 104/126 (82.54%), Query Frame = 0


Query: 6   MNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAFN 65
           MNFSSFTLYL+LL TISLN LL S   S +S  S+I + SSLHIVYT+ERPSNE+PEAF 
Sbjct: 1   MNFSSFTLYLVLLFTISLNSLLISAIISDKS-ASKIDVPSSLHIVYTTERPSNEEPEAFY 60

Query: 66  LQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQT 125
           +QIL SVLGS+EAAKKALVYSF +SM GFAA+LTPDQVKKI   PGV++V+Q VNY LQ 
Sbjct: 61  IQILASVLGSEEAAKKALVYSFKSSMNGFAADLTPDQVKKILAQPGVLNVLQGVNYELQA 120

Query: 126 EGKRDS 132
            G++++
Sbjct: 121 TGEKNN 125

[Close]

BLAST of Lsi03G006480 vs. NCBI nr
Match: ADN34190.1 (peptidase [Cucumis melo subsp. melo])

HSP 1 Score: 136.3 bits (342), Expect = 1.9e-28
Identity = 75/113 (66.37%), Postives = 91/113 (80.53%), Query Frame = 0


Query: 19  LTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAFNLQILTSVLGSKEA 78
           +++SLN LL+    S +S  S+  + SSLHIVYT++RPSNEKPEAF +QIL SVLGS EA
Sbjct: 19  VSVSLNSLLAWGMISEKS-MSKFAVPSSLHIVYTAKRPSNEKPEAFYIQILASVLGSNEA 78

Query: 79  AKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQTEGKRDS 132
           A+KALVYSF NSM GFAANLTP+QVKKIS  PGV+HV +SV YNLQT GK ++
Sbjct: 79  ARKALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVTYNLQTGGKENN 130

[Close]

BLAST of Lsi03G006480 vs. NCBI nr
Match: XP_038896852.1 (subtilisin-like protease SBT3.6 isoform X2 [Benincasa hispida])

HSP 1 Score: 124.8 bits (312), Expect = 5.7e-25
Identity = 62/90 (68.89%), Postives = 78/90 (86.67%), Query Frame = 0


Query: 42  GLLSSLHIVYTSERPSNEKPEAFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPD 101
           G  +++HIVYT ERP NE+PEA++++ L SVLGS+EAAKKALVYSF +S TGFAANLTPD
Sbjct: 3   GQSTAVHIVYT-ERPHNEEPEAYHIRTLASVLGSEEAAKKALVYSFTSSTTGFAANLTPD 62

Query: 102 QVKKISEHPGVVHVVQSVNYNLQTEGKRDS 132
           QV+KI   PGV+HVVQSVNY+LQT GK+++
Sbjct: 63  QVEKILAQPGVLHVVQSVNYDLQTRGKKNN 91

[Close]

BLAST of Lsi03G006480 vs. TAIR 10
Match: AT1G71950.1 (Proteinase inhibitor, propeptide )

HSP 1 Score: 81.6 bits (200), Expect = 5.1e-16
Identity = 45/114 (39.47%), Postives = 76/114 (66.67%), Query Frame = 0


Query: 14  YLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPEAFNLQILTSVL 73
           +   L +   +++++   T   + +SE    + +HI+YT E+P++E+P+ ++L+ L+S L
Sbjct: 20  FFFCLSSFFASIVMADEYTGEATGSSE----AKVHIIYT-EKPTDEEPKTYHLRTLSSAL 79

Query: 74  GSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQTEG 128
           GS+EAAK AL+YS+  + +GF+A LTP+QV +IS+ PGV+ VV S  Y L   G
Sbjct: 80  GSEEAAKDALIYSYKEAASGFSAKLTPEQVAEISKQPGVIQVVPSQTYQLHKPG 128

[Close]

BLAST of Lsi03G006480 vs. TAIR 10
Match: AT5G11940.1 (Subtilase family protein )

HSP 1 Score: 71.6 bits (174), Expect = 5.3e-13
Identity = 38/84 (45.24%), Postives = 58/84 (69.05%), Query Frame = 0


Query: 45  SSLHIVYTSERPSNEKPE---AFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPD 104
           + +HIVY  E+  N+ PE   + +L++L S+LGSK+ A +++V+S+ N  +GFAA+LT  
Sbjct: 35  TKVHIVYLGEKEHND-PELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDS 94

Query: 105 QVKKISEHPGVVHVVQSVNYNLQT 126
           Q ++ISEHP VV V  +  Y LQT
Sbjct: 95  QAEQISEHPDVVQVTPNTFYELQT 117

[Close]

BLAST of Lsi03G006480 vs. TAIR 10
Match: AT4G21650.1 (Subtilase family protein )

HSP 1 Score: 65.9 bits (159), Expect = 2.9e-11
Identity = 51/123 (41.46%), Postives = 74/123 (60.16%), Query Frame = 0


Query: 6   MNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPE--- 65
           MN S  +  L+LLL I+L L L +T     +    +   S ++IVY  ER  ++ PE   
Sbjct: 1   MNNSLQSSKLVLLLAIALVLFL-NTELDFLTAAGALDSDSKVYIVYLGER-EHDDPELVT 60

Query: 66  AFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYN 125
           A + Q+L S+L SKE A+ +L+YS+ +  +GFAA LT  Q KKISEHP V+HV+ +    
Sbjct: 61  ASHHQMLESLLQSKEDAQNSLIYSYQHGFSGFAALLTSSQAKKISEHPEVIHVIPNRIRK 120

[Close]

BLAST of Lsi03G006480 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 65.9 bits (159), Expect = 2.9e-11
Identity = 40/97 (41.24%), Postives = 57/97 (58.76%), Query Frame = 0


Query: 32  TSVRSTTSEIGLLSSLHIVYTSERPSNEKPE---AFNLQILTSVLGSKEAAKKALVYSFN 91
           T  RS  +E      +HIVY  E+  ++ PE     + ++L S+LGSKE A  ++VYS+ 
Sbjct: 35  TKERSFVAESSAKRKVHIVYLGEK-QHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYR 94

Query: 92  NSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYNLQT 126
           +  +GFAA LT  Q KKI++ P VVHV+    Y L T
Sbjct: 95  HGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLAT 130

[Close]

BLAST of Lsi03G006480 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.1e-10
Identity = 47/123 (38.21%), Postives = 71/123 (57.72%), Query Frame = 0


Query: 6   MNFSSFTLYLLLLLTISLNLLLSSTTTSVRSTTSEIGLLSSLHIVYTSERPSNEKPE--- 65
           MN+ + ++Y++L L I LN+         RS  +E      +HIVY  E+  ++ PE   
Sbjct: 2   MNYRT-SIYVVLSLVIFLNV--------QRSFVAESSAKRKVHIVYLGEK-QHDDPEFVT 61

Query: 66  AFNLQILTSVLGSKEAAKKALVYSFNNSMTGFAANLTPDQVKKISEHPGVVHVVQSVNYN 125
             + ++L S+LGSKE A  ++VYS+ +  +GFAA LT  Q KKI++ P VVHV+    Y 
Sbjct: 62  ESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYK 114

The following BLAST results are available for this feature:

BLAST of Lsi03G006480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1PDX5	7.5e-12	45.24	Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2...	[more]
Q8GUK4	4.1e-10	41.46	Subtilisin-like protease SBT3.13 OS=Arabidopsis thaliana OX=3702 GN=SBT3.13 PE=2...	[more]
Q8L7I2	1.6e-09	38.21	Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S...	[more]
Q9MAP5	2.0e-09	39.25	Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S...	[more]
F4HPF1	4.5e-09	35.14	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S...	[more]

BLAST of Lsi03G006480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C9P1	7.7e-36	68.70	Subtilisin-like protease SBT3.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3CFT4	7.7e-36	68.70	subtilisin-like protease SBT3.4 OS=Cucumis melo OX=3656 GN=LOC103500439 PE=4 SV=...	[more]
A0A0A0K630	1.0e-32	64.89	Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G27468...	[more]
E5GCJ1	9.1e-29	66.37	Peptidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A6J1CPA2	7.0e-21	52.85	subtilisin-like protease SBT3.11 isoform X1 OS=Momordica charantia OX=3673 GN=LO...	[more]

BLAST of Lsi03G006480 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896488.1	2.0e-38	73.28	subtilisin-like protease SBT3.13 [Benincasa hispida]	[more]
XP_008461963.1	1.6e-35	68.70	PREDICTED: subtilisin-like protease SBT3.4 [Cucumis melo] >KAA0048207.1 subtilis...	[more]
XP_038896487.1	1.1e-33	69.05	subtilisin-like protease SBT3.13 [Benincasa hispida]	[more]
ADN34190.1	1.9e-28	66.37	peptidase [Cucumis melo subsp. melo]	[more]
XP_038896852.1	5.7e-25	68.89	subtilisin-like protease SBT3.6 isoform X2 [Benincasa hispida]	[more]

BLAST of Lsi03G006480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71950.1	5.1e-16	39.47	Proteinase inhibitor, propeptide	[more]
AT5G11940.1	5.3e-13	45.24	Subtilase family protein	[more]
AT4G21650.1	2.9e-11	41.46	Subtilase family protein	[more]
AT4G10550.3	2.9e-11	41.24	Subtilase family protein	[more]
AT4G10550.1	1.1e-10	38.21	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 67..123 e-value: 3.1E-12 score: 47.0
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 29..124 e-value: 5.4E-20 score: 73.6
None	No IPR available	PANTHER	PTHR48222	PROTEINASE INHIBITOR, PROPEPTIDE	coord: 43..126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G006480.1	Lsi03G006480.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008233	peptidase activity