Lsi03G005810 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G005810
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	GH10 domain-containing protein
Location	chr03: 7146094 .. 7148203 (+)
RNA-Seq Expression	Lsi03G005810
Synteny	Lsi03G005810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGTCGGGCAATGTCGATTACTCCATTTTCGACGCCATGATGGACTTCACCAAGCACCACAACATCGTCGTTCGTGGCCACAATATCTTTTGGGACGATTAACAATATCAACAAGTCTGGCTGAAATCTCTCTCGATGAGAGATCTCTACCGTGCCGCCTGACGGAGACTCAATTCGGTCATGTCTAAGTATCGAGGGCAAGTGATTGCTTGGATGTGGAGAATGAGAATCTCCATTTGATTTCTTTGAGAGCAAATTAGGGCGGAGTGCTTCCGGTTTGTTCTATAATTGGGCGATGAAAGTCAATCAATCGGTTACATTGTTTCTTTATGAGTTTAATACCATTGAGTATAGCGGCGATGCGATTTCTTCTCCAGCTAGGTATTTTCAGAAGCTAGATGCTATTAGACGATTTCTTGGTAATAGTGGCGGCAGATTTGTGATTGGACTGGAATCACATTTCGGTCTTTCTCCTAATATTCCTTATATGAGATCCACCATTAATACGCTTGGTTCTGTTGGAATTCCGATTTGGCTCACCGAAGTAGATGTTTATAACTTGCAAAATCAAGTACAGTTCTTGAAATCTTACTCAAACAAATTAATGTTTATTCTGCCAACTTACTGTTGAATTTATCTGAATTTTGAATCGAAATCATTGTAGGATACATAATAAATCACATCTAGAACCATTAGCTTGTCTTGTTCATACATGTCTTGCTTTACAAAAATCAAATATATATTAATGCATTAGCATTTGCTTTGGTCAAGTGATAGTGTAATTTAGTGGTATGCGTACGTGAGTGAGATTGTTGAGAATTCATATAAAGGATGAAACATACTCAAATTCAAATATATTAGCTTTACTTTTGGGTGAGGTTAGGTTTATAAATGTAGTTGGGCTAAGAATTCCAACATATAAACTATTAAACTCATAGGAACTTCTACTCTGTTTAAGGAGTTGATTCTACATGCATTAAAAGTGATTAGTATATATTTGAGAGTGATCTTGAAATGATTAAAATCACTTTTGTCATGTTTAAAATCACTCGGACACATAGTCTTAATTACTTGAAATCGACTCAATACTTATTTTTACTCTTTTAAATGTATTTTCCATACTATTAAAATTGATTTTGAATGATTAAAAATATTTTCTGAGTGATCTTAACTATTTTAGAATCACTTCCAAACATGCTGTTAGAGTTTGAAATCACTTTTTCGATCAAGTTACTTTGTAAAATCTAGTTGACGGTAGCTTTTGAGATTGACATGCCAAAGAATATGGTGTCTCTTCTAACTAACTCCTAAAAATTCCTATTGTATCCTACTTTATTTTTTACGCTTACTTCATATTACATTTGATTTTTCTAAAAGTACATTGACCAACAATTATTTTAAAAGCTTCATGAATCCCAAACAGCCAAATTAAACTAAGTGATCTAAATATCATTCCACTCTTAGATTTAAAGGTAAGATTAGATGTAGATTAGCAATACAAATCAAGTCATTCATACTGATTAGCTTGTAGAATTGAAGCTTCCTCCTAGTTATTGCAAACTATCTCAATTTGTTAGAGCTCAATATAAAAGATGTTAAAAGAAACAAAAAGATGATGATGATGATGGGCTGGGGCAACCCAATTGTTTGTAAATGGGTCTCATAATCAATTGACATAAGAATTTTGTGAATTTCACATGATGATATGATATGTTATGATATGATATGATATGATATGATATTCTGGGTTTTGTGTGTTGATGAAAACAGGCATTTAATTTGGAGCAAGTTCTAAGAGAAGGACTTTCTCATCTAAAAGTGAATGCAATTGTGATTTGGTTAGTATGGTCACCCGGGGATGTTATCGAATGTGTTTGACAGACAACAACTTCAACAATTTACCAACTGGTAATGTGTGGTAGACAAGTTATTAAAAGAGTGGGGGATCAAAGGCTCAATCACTGCAACAATAGTGTCAATGGCTTCTTTGAAGCCTCACTTTTCCATGGAGAATATGAAATACAAATCTCTCACCCGTTTGTCACCAATTCCTCTCTCAATACTCAGAAGTTTAGTGTTCTTGCTACATCAGAAGGTGAATTTGAATAC

mRNA sequence

AATGTCGGGCAATGTCGATTACTCCATTTTCGACGCCATGATGGACTTCACCAAGCACCACAACATCGTCGTTCGTGGCCACAATATCTTTTGGGACGATTAACAATATCAACAAGTCTGGCTGAAATCTCTCTCGATGAGAGATCTCTACCGTGCCGCCTGACGGAGACTCAATTCGGGCGGAGTGCTTCCGGTTTGTTCTATAATTGGGCGATGAAAGTCAATCAATCGGTTACATTGTTTCTTTATGAGTTTAATACCATTGAGTATAGCGGCGATGCGATTTCTTCTCCAGCTAGGTATTTTCAGAAGCTAGATGCTATTAGACGATTTCTTGGTAATAGTGGCGGCAGATTTGTGATTGGACTGGAATCACATTTCGGTCTTTCTCCTAATATTCCTTATATGAGATCCACCATTAATACGCTTGGTTCTGTTGGAATTCCGATTTGGCTCACCGAAGTAGATGTTTATAACTTGCAAAATCAAACAACAACTTCAACAATTTACCAACTGGTAATGTGTGGTAGACAAGTTATTAAAAGAGTGGGGGATCAAAGGCTCAATCACTGCAACAATAGTGTCAATGGCTTCTTTGAAGCCTCACTTTTCCATGGAGAATATGAAATACAAATCTCTCACCCGTTTGTCACCAATTCCTCTCTCAATACTCAGAAGTTTAGTGTTCTTGCTACATCAGAAGGTGAATTTGAATAC

Coding sequence (CDS)

AATGTCGGGCAATGTCGATTACTCCATTTTCGACGCCATGATGGACTTCACCAAGCACCACAACATCGTCGTTCGTGGCCACAATATCTTTTGGGACGATTAACAATATCAACAAGTCTGGCTGAAATCTCTCTCGATGAGAGATCTCTACCGTGCCGCCTGACGGAGACTCAATTCGGGCGGAGTGCTTCCGGTTTGTTCTATAATTGGGCGATGAAAGTCAATCAATCGGTTACATTGTTTCTTTATGAGTTTAATACCATTGAGTATAGCGGCGATGCGATTTCTTCTCCAGCTAGGTATTTTCAGAAGCTAGATGCTATTAGACGATTTCTTGGTAATAGTGGCGGCAGATTTGTGATTGGACTGGAATCACATTTCGGTCTTTCTCCTAATATTCCTTATATGAGATCCACCATTAATACGCTTGGTTCTGTTGGAATTCCGATTTGGCTCACCGAAGTAGATGTTTATAACTTGCAAAATCAAACAACAACTTCAACAATTTACCAACTGGTAATGTGTGGTAGACAAGTTATTAAAAGAGTGGGGGATCAAAGGCTCAATCACTGCAACAATAGTGTCAATGGCTTCTTTGAAGCCTCACTTTTCCATGGAGAATATGAAATACAAATCTCTCACCCGTTTGTCACCAATTCCTCTCTCAATACTCAGAAGTTTAGTGTTCTTGCTACATCAGAAGGTGAATTTGAATAC

Protein sequence

NVGQCRLLHFRRHDGLHQAPQHRRSWPQYLLGRLTISTSLAEISLDERSLPCRLTETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNSGGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQTTTSTIYQLVMCGRQVIKRVGDQRLNHCNNSVNGFFEASLFHGEYEIQISHPFVTNSSLNTQKFSVLATSEGEFEY

Homology

[Close]

BLAST of Lsi03G005810 vs. ExPASy Swiss-Prot
Match: A0A1P8B8F8 (Endo-1,4-beta-xylanase 5 OS=Arabidopsis thaliana OX=3702 GN=XYN5 PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 5.4e-21
Identity = 67/220 (30.45%), Postives = 102/220 (46.36%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E   G +AS  FYN A K++  VT+F+ E+NTIE   +  ++P +  +K++ I  + GN 
Sbjct: 340 EKMLGANASSSFYNLAFKLDPDVTMFVNEYNTIENRVEVTATPVKVKEKMEEILAYPGNM 399

Query: 116 GGRFVIGLESHF-GLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQ----------- 175
             +  IG + HF    PN+ YMRS ++TLGS+G+PIWLTEVD+    NQ           
Sbjct: 400 NIKGAIGAQGHFRPTQPNLAYMRSALDTLGSLGLPIWLTEVDMPKCPNQEVYIEEILREA 459

Query: 176 -----------------------TTTSTIYQLVMCGRQVIKRVGDQRLN------HCNNS 235
                                  T     +     G  + K + + + +         +S
Sbjct: 460 YSHPAVKGIIIFAGPEVSGFDKLTLADKYFNNTATGDVIDKLLKEWQQSSEIPKIFMTDS 519

[Close]

BLAST of Lsi03G005810 vs. ExPASy Swiss-Prot
Match: Q84WT5 (Endo-1,4-beta-xylanase 5-like OS=Arabidopsis thaliana OX=3702 GN=At4g33820 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 7.0e-21
Identity = 66/223 (29.60%), Postives = 105/223 (47.09%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E   G +AS  FYN A K++  V LF+ E+NTIE + +  ++P +  + ++ I  + GN 
Sbjct: 340 EKMLGANASTSFYNLAFKIDPDVRLFVNEYNTIENTKEFTATPIKVKKMMEEILAYPGNK 399

Query: 116 GGRFVIGLESHFG-LSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQ----------- 175
             +  IG + HFG   PN+ Y+RS ++TLGS+G+PIWLTEVD+    NQ           
Sbjct: 400 NMKGAIGAQGHFGPTQPNLAYIRSALDTLGSLGLPIWLTEVDMPKCPNQAQYVEDILREA 459

Query: 176 --------------------------------TTTSTIYQLVMCGRQVIKRVGDQRLNHC 235
                                           T T  +   ++  ++  ++  + + N  
Sbjct: 460 YSHPAVKGIIIFGGPEVSGFDKLTLADKDFNNTQTGDVIDKLL--KEWQQKSSEIQTNFT 519

[Close]

BLAST of Lsi03G005810 vs. ExPASy Swiss-Prot
Match: Q680B7 (Endo-1,4-beta-xylanase 4 OS=Arabidopsis thaliana OX=3702 GN=XYN4 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 6.6e-19
Identity = 45/109 (41.28%), Postives = 67/109 (61.47%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E   G +AS + Y+ A K++  + LFL EFNT+EY  D + SP    +K+  I  F GN+
Sbjct: 333 ENMLGGNASAIVYSLASKLDPDIPLFLNEFNTVEYDKDRVVSPVNVVKKMQEIVSFPGNN 392

Query: 116 GGRFVIGLESHFG-LSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQ 164
             +  IG + HF  + PN+ YMR  ++TLGS+  P+WLTEVD++   +Q
Sbjct: 393 NIKGGIGAQGHFAPVQPNLAYMRYALDTLGSLSFPVWLTEVDMFKCPDQ 441

[Close]

BLAST of Lsi03G005810 vs. ExPASy Swiss-Prot
Match: O80596 (Endo-1,4-beta-xylanase 2 OS=Arabidopsis thaliana OX=3702 GN=XYN2 PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.4e-05
Identity = 31/90 (34.44%), Postives = 53/90 (58.89%), Query Frame = 0


Query: 68  YNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNSGGRFVIGLESHF 127
           +  A +++   TLFL E++ IE   D+ SSP +Y + +  +++     GG   IG++ H 
Sbjct: 860 FKTAHELDPLATLFLNEYH-IEDGFDSRSSPEKYIKLVHKLQKKGAPVGG---IGIQGHI 919

Query: 128 GLSPNIPYMRSTINTLGSVGIPIWLTEVDV 158
             SP    +RS ++ L ++G+PIW TE+DV
Sbjct: 920 -TSPVGHIVRSALDKLSTLGLPIWFTELDV 944

[Close]

BLAST of Lsi03G005810 vs. ExPASy Swiss-Prot
Match: A0A1P8AWH8 (Endo-1,4-beta-xylanase 1 OS=Arabidopsis thaliana OX=3702 GN=XYN1 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 5.4e-05
Identity = 31/102 (30.39%), Postives = 56/102 (54.90%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           + + G+      +  A +++ S TLF+ +++ IE   D  S P +Y +++  ++      
Sbjct: 726 QDKLGKDIRVNMFKTAHQLDPSATLFVNDYH-IEDGCDPKSCPEKYTEQILDLQEKGAPV 785

Query: 116 GGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDV 158
           GG   IG++ H   SP  P + S ++ LG +G+PIW TE+DV
Sbjct: 786 GG---IGIQGHID-SPVGPIVCSALDKLGILGLPIWFTELDV 822

[Close]

BLAST of Lsi03G005810 vs. ExPASy TrEMBL
Match: A0A6J1GMR3 (uncharacterized protein LOC111455386 OS=Cucurbita moschata OX=3662 GN=LOC111455386 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 3.6e-52
Identity = 114/218 (52.29%), Postives = 138/218 (63.30%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E + G +ASG+FYNWAMK + S+ LF+ ++NTIEYSGDA SSPA+Y QKLD+IRR+ GNS
Sbjct: 350 ERKLGSAASGMFYNWAMKRDGSIPLFMNDYNTIEYSGDAASSPAKYLQKLDSIRRYSGNS 409

Query: 116 GGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQT----------- 175
           GGRF IGLESHFG SPNIPYMRS I+TLGS G+PIWLTEVDV N  NQ            
Sbjct: 410 GGRFAIGLESHFGPSPNIPYMRSAIDTLGSAGVPIWLTEVDVSNSPNQAQDLERVLREGF 469

Query: 176 --------------TTSTIYQLVMCGR------------QVIKRVGDQRLNHCNNSVNGF 235
                         + S  Y++ +               +++K  G +         NGF
Sbjct: 470 AHPKVNGIVIWSAWSPSGCYRMCLTDNNFNNLATGNVVDKLLKEWGIKTSITATTDANGF 529

Query: 236 FEASLFHGEYEIQISHPFVTNSSLNTQKFSVLATSEGE 237
           FEASLFHG+YE+QI+HP VTNSSLN  KFSVLA  E E
Sbjct: 530 FEASLFHGDYEMQIAHPSVTNSSLNAHKFSVLAAQEQE 567

[Close]

BLAST of Lsi03G005810 vs. ExPASy TrEMBL
Match: A0A0A0K6B7 (GH10 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G071360 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 5.2e-51
Identity = 118/231 (51.08%), Postives = 139/231 (60.17%), Query Frame = 0


Query: 45  LDERSLPCRLTETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQK 104
           ++  +L     E++ G  ASGLFYNWAMK +QS+ LFL EFNTIE SGDA SSPARY QK
Sbjct: 343 VENENLHFNFFESKLGWPASGLFYNWAMKADQSIPLFLNEFNTIESSGDAASSPARYLQK 402

Query: 105 LDAIRRFLGNSGGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQT 164
           LD IR+F GN GGRF IGLESHFG SPNI YMRS I+TLGS G+PIWLTEVDV N  NQ 
Sbjct: 403 LDTIRKFPGNRGGRFAIGLESHFGPSPNIAYMRSAIDTLGSAGVPIWLTEVDVSNSANQA 462

Query: 165 ----------------------------------TTSTIYQLVMCG---RQVIKRVGDQR 224
                                              T   ++ +  G    +++K  G + 
Sbjct: 463 YNLEQVLREGFSHPKVNGIVIWSAWAPWGCYRMCLTDNNFRNLPTGDVVDKLLKEWGIKG 522

Query: 225 LNHCNNSVNGFFEASLFHGEYEIQISHPFVTNSSLNTQKFSVLATSEGEFE 239
                   NGFFEASLFHGEYE++ISHP V  SS++ QKFSVL  SEGE E
Sbjct: 523 SITATTDSNGFFEASLFHGEYEMKISHPSVAKSSIDAQKFSVLPASEGESE 573

[Close]

BLAST of Lsi03G005810 vs. ExPASy TrEMBL
Match: A0A6J1I4U7 (uncharacterized protein LOC111471011 OS=Cucurbita maxima OX=3661 GN=LOC111471011 PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 6.8e-51
Identity = 113/218 (51.83%), Postives = 136/218 (62.39%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E + G +ASG+FYNWAMK + S+ LF+ E+NTIE SGDA SSPA+Y QKLD+IR + GNS
Sbjct: 350 ERKLGSAASGMFYNWAMKRDGSIPLFMNEYNTIENSGDAASSPAKYLQKLDSIRHYSGNS 409

Query: 116 GGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQT----------- 175
           GGRF IGLESHFG SPNIPYMRS I+TLGS G+PIWLTE+DV N  NQ            
Sbjct: 410 GGRFAIGLESHFGPSPNIPYMRSAIDTLGSAGVPIWLTEIDVSNSPNQAQDLEQALREGF 469

Query: 176 --------------TTSTIYQLVMCGR------------QVIKRVGDQRLNHCNNSVNGF 235
                         + S  Y++ +               +++K  G +         NGF
Sbjct: 470 AHPKVNGIVIWSAWSPSGCYRMCLTDNNFNNLATGNVVDKLLKEWGIKDSITATTDANGF 529

Query: 236 FEASLFHGEYEIQISHPFVTNSSLNTQKFSVLATSEGE 237
           FEASLFHG+YE+QISHP VTN SLN QKFSVLA  E E
Sbjct: 530 FEASLFHGDYEMQISHPSVTNPSLNAQKFSVLAAPEQE 567

[Close]

BLAST of Lsi03G005810 vs. ExPASy TrEMBL
Match: A0A6J1D3W0 (uncharacterized protein LOC111017351 OS=Momordica charantia OX=3673 GN=LOC111017351 PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 8.9e-51
Identity = 114/231 (49.35%), Postives = 139/231 (60.17%), Query Frame = 0


Query: 45  LDERSLPCRLTETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQK 104
           ++   L  R  E + G  ASGLFYNWAMK + S+ LFL ++NTIE  GDA SSPARY QK
Sbjct: 340 VENEDLHFRFFEARLGGRASGLFYNWAMKADGSIPLFLNDYNTIESRGDASSSPARYLQK 399

Query: 105 LDAIRRFLGNSGGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQT 164
           LD IRRF GNSGGRF IGLESHFG  P+I YMRS I+TLGS G+PIWLTEVDV ++ NQ 
Sbjct: 400 LDEIRRFPGNSGGRFAIGLESHFGSPPDIAYMRSAIDTLGSAGVPIWLTEVDVSSMPNQA 459

Query: 165 -------------------------TTSTIYQLVMCGR------------QVIKRVGDQR 224
                                    +    Y++ +               +++K  G + 
Sbjct: 460 YYLEQVLREGFSHPKVNGIVIWSAWSPGGCYRMCLTDNNFHNLPTGDVVDKLLKEWGIKA 519

Query: 225 LNHCNNSVNGFFEASLFHGEYEIQISHPFVTNSSLNTQKFSVLATSEGEFE 239
                   NGFFEASLFHG+Y++QISHP VTNSSLN QK SV+A SEG+ E
Sbjct: 520 SITATTDANGFFEASLFHGDYDVQISHPSVTNSSLNAQKLSVVAASEGDSE 570

[Close]

BLAST of Lsi03G005810 vs. ExPASy TrEMBL
Match: A0A5D3C4I2 (Endoxylanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001250 PE=3 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 5.7e-50
Identity = 124/279 (44.44%), Postives = 153/279 (54.84%), Query Frame = 0


Query: 11  RRHDGLHQAPQHRRSWPQ-------YLLGRLTISTSLAEI-------SLDERSLPCRLTE 70
           R H+     PQ+ + W +       Y   R  + + +++         ++  +L     E
Sbjct: 295 RGHNVFWNDPQYVQGWVKSLSNTDLYRAARRRLDSVMSKYRGQVIAWDVENENLHFNFFE 354

Query: 71  TQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNSG 130
           ++ G  ASGLFYNWAMK +QS+ LFL EFNTIE SGDA SSPARY QKLDAIR+F GNS 
Sbjct: 355 SKLGWPASGLFYNWAMKADQSIPLFLNEFNTIESSGDAASSPARYLQKLDAIRKFPGNSD 414

Query: 131 GRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQT------------ 190
            RF IGLESHFG SPN+ YMRS I+TLGS G+PIWLTEVDV N  NQ             
Sbjct: 415 ARFAIGLESHFGPSPNLAYMRSAIDTLGSAGVPIWLTEVDVSNSANQAYDLEQVLREGFS 474

Query: 191 ----------------------TTSTIYQLVMCG---RQVIKRVGDQRLNHCNNSVNGFF 239
                                  T   +  +  G    +++K  G +         NGFF
Sbjct: 475 HPKVNGIVIWSAWAPWGCYRMCLTDNNFNNLPTGDVVDKLLKEWGIKGSITATTDSNGFF 534

[Close]

BLAST of Lsi03G005810 vs. NCBI nr
Match: XP_038887131.1 (endo-1,4-beta-xylanase 5-like [Benincasa hispida])

HSP 1 Score: 225.3 bits (573), Expect = 5.5e-55
Identity = 125/220 (56.82%), Postives = 138/220 (62.73%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E++ G  ASGLFYNWAMK +QS+ LFL EFNTIEYSGDA SSPARY QKLDAIRRF GNS
Sbjct: 352 ESKLGLGASGLFYNWAMKADQSIPLFLNEFNTIEYSGDAASSPARYLQKLDAIRRFPGNS 411

Query: 116 GGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQT----------- 175
           GGRF IGLESHFG +PNIPYMRS I+TLGS G+PIWLTEVDV N  NQ            
Sbjct: 412 GGRFAIGLESHFGPNPNIPYMRSAIDTLGSAGVPIWLTEVDVSNSPNQAYNLEQVLREGF 471

Query: 176 -----------------------TTSTIYQLVMCGRQVIKRVGDQRLNHC---NNSVNGF 235
                                   T   +  +  G  V K + +  +          NGF
Sbjct: 472 SHPKVNGIVIWSAWSPGGCYRMCLTDNNFHNLPTGDVVDKLLQEWGIKGSITGTTDGNGF 531

Query: 236 FEASLFHGEYEIQISHPFVTNSSLNTQKFSVLATSEGEFE 239
           FEASLFHGEYE+QISHP VT SSLN QKFSV A SEGE E
Sbjct: 532 FEASLFHGEYEMQISHPSVTESSLNAQKFSVFAASEGESE 571

[Close]

BLAST of Lsi03G005810 vs. NCBI nr
Match: XP_023553798.1 (uncharacterized protein LOC111811263 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 216.5 bits (550), Expect = 2.6e-52
Identity = 115/218 (52.75%), Postives = 138/218 (63.30%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E + G +ASG+FYNWAMK + S+ LF+ E+NTIEYSGDA SSPA+Y QKLD+IRR+ GNS
Sbjct: 349 ERKLGSAASGMFYNWAMKTDGSIPLFMNEYNTIEYSGDAASSPAKYLQKLDSIRRYSGNS 408

Query: 116 GGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQT----------- 175
           GGRF IGLESHFG SPNIPYMRS I+TLGS G+PIWLTEVDV N  NQ            
Sbjct: 409 GGRFAIGLESHFGPSPNIPYMRSAIDTLGSAGVPIWLTEVDVSNSPNQAHDLEQVLREGF 468

Query: 176 --------------TTSTIYQLVMCGR------------QVIKRVGDQRLNHCNNSVNGF 235
                         + S  Y++ +               +++K  G +         NGF
Sbjct: 469 AHPKVNGIVIWSAWSPSGCYRMCLTDNNFNNLATGNVVDKLLKEWGIKDSITATTDANGF 528

Query: 236 FEASLFHGEYEIQISHPFVTNSSLNTQKFSVLATSEGE 237
           FEASLFHG+YE+QI+HP VTN SLN QKFSVLA  E E
Sbjct: 529 FEASLFHGDYEMQIAHPSVTNPSLNAQKFSVLAAPEQE 566

[Close]

BLAST of Lsi03G005810 vs. NCBI nr
Match: XP_022952795.1 (uncharacterized protein LOC111455386 [Cucurbita moschata])

HSP 1 Score: 214.9 bits (546), Expect = 7.4e-52
Identity = 114/218 (52.29%), Postives = 138/218 (63.30%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E + G +ASG+FYNWAMK + S+ LF+ ++NTIEYSGDA SSPA+Y QKLD+IRR+ GNS
Sbjct: 350 ERKLGSAASGMFYNWAMKRDGSIPLFMNDYNTIEYSGDAASSPAKYLQKLDSIRRYSGNS 409

Query: 116 GGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQT----------- 175
           GGRF IGLESHFG SPNIPYMRS I+TLGS G+PIWLTEVDV N  NQ            
Sbjct: 410 GGRFAIGLESHFGPSPNIPYMRSAIDTLGSAGVPIWLTEVDVSNSPNQAQDLERVLREGF 469

Query: 176 --------------TTSTIYQLVMCGR------------QVIKRVGDQRLNHCNNSVNGF 235
                         + S  Y++ +               +++K  G +         NGF
Sbjct: 470 AHPKVNGIVIWSAWSPSGCYRMCLTDNNFNNLATGNVVDKLLKEWGIKTSITATTDANGF 529

Query: 236 FEASLFHGEYEIQISHPFVTNSSLNTQKFSVLATSEGE 237
           FEASLFHG+YE+QI+HP VTNSSLN  KFSVLA  E E
Sbjct: 530 FEASLFHGDYEMQIAHPSVTNSSLNAHKFSVLAAQEQE 567

[Close]

BLAST of Lsi03G005810 vs. NCBI nr
Match: KAG6571918.1 (Endo-1,4-beta-xylanase 5-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 213.0 bits (541), Expect = 2.8e-51
Identity = 113/218 (51.83%), Postives = 137/218 (62.84%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E + G +ASG+FYNWAMK + S+ LF+ ++NTIEYSGDA SSPA+Y QKLD+IRR+ GNS
Sbjct: 350 ERKLGSAASGMFYNWAMKRDGSIPLFMNDYNTIEYSGDAASSPAKYLQKLDSIRRYSGNS 409

Query: 116 GGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQT----------- 175
           GGRF IGLESHFG SPNIPYMRS I+TLGS G+PIWLTEVDV N  NQ            
Sbjct: 410 GGRFAIGLESHFGPSPNIPYMRSAIDTLGSAGVPIWLTEVDVSNSPNQAQDLEQVLREGF 469

Query: 176 --------------TTSTIYQLVMCGR------------QVIKRVGDQRLNHCNNSVNGF 235
                         + S  Y++ +               +++K  G +         NGF
Sbjct: 470 AHPKVNGIVIWSAWSPSGCYRMCLTDNNFNNLATGNVVDKLLKEWGIKTSITATTDANGF 529

Query: 236 FEASLFHGEYEIQISHPFVTNSSLNTQKFSVLATSEGE 237
           FEASLFHG+YE+ I+HP VTNSSLN  KFSVLA  E E
Sbjct: 530 FEASLFHGDYEMHIAHPSVTNSSLNAHKFSVLAAQEQE 567

[Close]

BLAST of Lsi03G005810 vs. NCBI nr
Match: KGN43847.1 (hypothetical protein Csa_017047 [Cucumis sativus])

HSP 1 Score: 211.1 bits (536), Expect = 1.1e-50
Identity = 118/231 (51.08%), Postives = 139/231 (60.17%), Query Frame = 0


Query: 45  LDERSLPCRLTETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQK 104
           ++  +L     E++ G  ASGLFYNWAMK +QS+ LFL EFNTIE SGDA SSPARY QK
Sbjct: 343 VENENLHFNFFESKLGWPASGLFYNWAMKADQSIPLFLNEFNTIESSGDAASSPARYLQK 402

Query: 105 LDAIRRFLGNSGGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQT 164
           LD IR+F GN GGRF IGLESHFG SPNI YMRS I+TLGS G+PIWLTEVDV N  NQ 
Sbjct: 403 LDTIRKFPGNRGGRFAIGLESHFGPSPNIAYMRSAIDTLGSAGVPIWLTEVDVSNSANQA 462

Query: 165 ----------------------------------TTSTIYQLVMCG---RQVIKRVGDQR 224
                                              T   ++ +  G    +++K  G + 
Sbjct: 463 YNLEQVLREGFSHPKVNGIVIWSAWAPWGCYRMCLTDNNFRNLPTGDVVDKLLKEWGIKG 522

Query: 225 LNHCNNSVNGFFEASLFHGEYEIQISHPFVTNSSLNTQKFSVLATSEGEFE 239
                   NGFFEASLFHGEYE++ISHP V  SS++ QKFSVL  SEGE E
Sbjct: 523 SITATTDSNGFFEASLFHGEYEMKISHPSVAKSSIDAQKFSVLPASEGESE 573

[Close]

BLAST of Lsi03G005810 vs. TAIR 10
Match: AT4G33830.1 (Glycosyl hydrolase family 10 protein )

HSP 1 Score: 111.7 bits (278), Expect = 8.2e-25
Identity = 72/206 (34.95%), Postives = 106/206 (51.46%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E++FG +AS   +  A  ++ S T+F+ EF T+E   D  +SPA+Y +KL  ++      
Sbjct: 350 ESKFGPNASNNIFAMAHAIDPSTTMFMNEFYTLEDPTDLKASPAKYLEKLRELQSIRVRG 409

Query: 116 GGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVY----------------- 175
                IGLESHF  +PNIPYMRS ++TLG+ G+PIWLTE+DV                  
Sbjct: 410 NIPLGIGLESHFS-TPNIPYMRSALDTLGATGLPIWLTEIDVKAPSSDQAKYFEQVLREG 469

Query: 176 -----------------NLQNQTTTSTIYQLVMCG---RQVIKRVGDQRLNHCN-NSVNG 224
                            N  +   T   ++ +  G    ++I+  G  R         +G
Sbjct: 470 HAHPHVKGMVTWTAYAPNCYHMCLTDGNFKNLPTGDVVDKLIREWGGLRSQTTEVTDADG 529

[Close]

BLAST of Lsi03G005810 vs. TAIR 10
Match: AT4G33860.1 (Glycosyl hydrolase family 10 protein )

HSP 1 Score: 107.8 bits (268), Expect = 1.2e-23
Identity = 88/273 (32.23%), Postives = 125/273 (45.79%), Query Frame = 0


Query: 11  RRHDGLHQAPQHRRSWPQYLLG--------RLTIST------SLAEISLDERSLPCRLTE 70
           R H+ L   P+++  W   L G        R   S        LA   +   +L     E
Sbjct: 290 RGHNILWNDPKYQPKWVNALSGNDLYNAVKRRVFSVVSRYKGQLAGWDVVNENLHFSYFE 349

Query: 71  TQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIR--RFLGN 130
            + G  AS   +  A   + + T+F+ E+NT+E S D+ SS ARY QKL  IR  R  GN
Sbjct: 350 DKMGPKASYNIFKMAQAFDPTTTMFMNEYNTLEESSDSDSSLARYLQKLREIRSIRVCGN 409

Query: 131 SGGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDVY---NLQ---------- 190
                 IGLESHF  +PNIPYMRS ++TL + G+PIWLTEVDV    N+Q          
Sbjct: 410 IS--LGIGLESHF-KTPNIPYMRSALDTLAATGLPIWLTEVDVEAPPNVQAKYFEQVLRE 469

Query: 191 ----------------------NQTTTSTIYQLVMCGRQVIKRV----GDQRLNHCNNSV 229
                                     T   ++ V  G  V K +    G +R        
Sbjct: 470 GHAHPQVKGIVTWSGYSPSGCYRMCLTDGNFKNVPTGDVVDKLLHEWGGFRRQTTGVTDA 529

[Close]

BLAST of Lsi03G005810 vs. TAIR 10
Match: AT4G33840.1 (Glycosyl hydrolase family 10 protein )

HSP 1 Score: 105.5 bits (262), Expect = 5.9e-23
Identity = 72/204 (35.29%), Postives = 97/204 (47.55%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E++FG  AS   Y  A  V+    +F+ E+NT+E   D  SSPARY  KL  ++      
Sbjct: 349 ESKFGPKASYNTYTMAHAVDPRTPMFMNEYNTLEQPKDLTSSPARYLGKLRELQSIRVAG 408

Query: 116 GGRFVIGLESHFGLSPNIPYMRSTINTLGSVGIPIWLTEVDV------------------ 175
                IGLESHF  +PNIPYMRS ++T G+ G+PIWLTE+DV                  
Sbjct: 409 KIPLAIGLESHFS-TPNIPYMRSALDTFGATGLPIWLTEIDVDAPPNVRANYFEQVLREG 468

Query: 176 --YNLQNQTTTSTIYQLVMCGR------------------QVIKRVGDQRLNHCN-NSVN 221
             +   N     T Y    C R                  ++++  G  R         N
Sbjct: 469 HAHPKVNGMVMWTGYSPSGCYRMCLTDGNFKNLPTGDVVDKLLREWGGLRSQTTGVTDAN 528

[Close]

BLAST of Lsi03G005810 vs. TAIR 10
Match: AT4G33810.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 3.8e-22
Identity = 67/220 (30.45%), Postives = 102/220 (46.36%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E   G +AS  FYN A K++  VT+F+ E+NTIE   +  ++P +  +K++ I  + GN 
Sbjct: 300 EKMLGANASSSFYNLAFKLDPDVTMFVNEYNTIENRVEVTATPVKVKEKMEEILAYPGNM 359

Query: 116 GGRFVIGLESHF-GLSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQ----------- 175
             +  IG + HF    PN+ YMRS ++TLGS+G+PIWLTEVD+    NQ           
Sbjct: 360 NIKGAIGAQGHFRPTQPNLAYMRSALDTLGSLGLPIWLTEVDMPKCPNQEVYIEEILREA 419

Query: 176 -----------------------TTTSTIYQLVMCGRQVIKRVGDQRLN------HCNNS 235
                                  T     +     G  + K + + + +         +S
Sbjct: 420 YSHPAVKGIIIFAGPEVSGFDKLTLADKYFNNTATGDVIDKLLKEWQQSSEIPKIFMTDS 479

[Close]

BLAST of Lsi03G005810 vs. TAIR 10
Match: AT4G33820.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 5.0e-22
Identity = 66/223 (29.60%), Postives = 105/223 (47.09%), Query Frame = 0


Query: 56  ETQFGRSASGLFYNWAMKVNQSVTLFLYEFNTIEYSGDAISSPARYFQKLDAIRRFLGNS 115
           E   G +AS  FYN A K++  V LF+ E+NTIE + +  ++P +  + ++ I  + GN 
Sbjct: 340 EKMLGANASTSFYNLAFKIDPDVRLFVNEYNTIENTKEFTATPIKVKKMMEEILAYPGNK 399

Query: 116 GGRFVIGLESHFG-LSPNIPYMRSTINTLGSVGIPIWLTEVDVYNLQNQ----------- 175
             +  IG + HFG   PN+ Y+RS ++TLGS+G+PIWLTEVD+    NQ           
Sbjct: 400 NMKGAIGAQGHFGPTQPNLAYIRSALDTLGSLGLPIWLTEVDMPKCPNQAQYVEDILREA 459

Query: 176 --------------------------------TTTSTIYQLVMCGRQVIKRVGDQRLNHC 235
                                           T T  +   ++  ++  ++  + + N  
Sbjct: 460 YSHPAVKGIIIFGGPEVSGFDKLTLADKDFNNTQTGDVIDKLL--KEWQQKSSEIQTNFT 519

The following BLAST results are available for this feature:

BLAST of Lsi03G005810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1P8B8F8	5.4e-21	30.45	Endo-1,4-beta-xylanase 5 OS=Arabidopsis thaliana OX=3702 GN=XYN5 PE=3 SV=1	[more]
Q84WT5	7.0e-21	29.60	Endo-1,4-beta-xylanase 5-like OS=Arabidopsis thaliana OX=3702 GN=At4g33820 PE=2 ...	[more]
Q680B7	6.6e-19	41.28	Endo-1,4-beta-xylanase 4 OS=Arabidopsis thaliana OX=3702 GN=XYN4 PE=2 SV=1	[more]
O80596	2.4e-05	34.44	Endo-1,4-beta-xylanase 2 OS=Arabidopsis thaliana OX=3702 GN=XYN2 PE=3 SV=1	[more]
A0A1P8AWH8	5.4e-05	30.39	Endo-1,4-beta-xylanase 1 OS=Arabidopsis thaliana OX=3702 GN=XYN1 PE=1 SV=1	[more]

BLAST of Lsi03G005810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GMR3	3.6e-52	52.29	uncharacterized protein LOC111455386 OS=Cucurbita moschata OX=3662 GN=LOC1114553...	[more]
A0A0A0K6B7	5.2e-51	51.08	GH10 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G071360 PE=3 S...	[more]
A0A6J1I4U7	6.8e-51	51.83	uncharacterized protein LOC111471011 OS=Cucurbita maxima OX=3661 GN=LOC111471011...	[more]
A0A6J1D3W0	8.9e-51	49.35	uncharacterized protein LOC111017351 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A5D3C4I2	5.7e-50	44.44	Endoxylanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001250 P...	[more]

BLAST of Lsi03G005810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887131.1	5.5e-55	56.82	endo-1,4-beta-xylanase 5-like [Benincasa hispida]	[more]
XP_023553798.1	2.6e-52	52.75	uncharacterized protein LOC111811263 [Cucurbita pepo subsp. pepo]	[more]
XP_022952795.1	7.4e-52	52.29	uncharacterized protein LOC111455386 [Cucurbita moschata]	[more]
KAG6571918.1	2.8e-51	51.83	Endo-1,4-beta-xylanase 5-like protein, partial [Cucurbita argyrosperma subsp. so...	[more]
KGN43847.1	1.1e-50	51.08	hypothetical protein Csa_017047 [Cucumis sativus]	[more]

BLAST of Lsi03G005810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G33830.1	8.2e-25	34.95	Glycosyl hydrolase family 10 protein	[more]
AT4G33860.1	1.2e-23	32.23	Glycosyl hydrolase family 10 protein	[more]
AT4G33840.1	5.9e-23	35.29	Glycosyl hydrolase family 10 protein	[more]
AT4G33810.1	3.8e-22	30.45	Glycosyl hydrolase superfamily protein	[more]
AT4G33820.1	5.0e-22	29.60	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 42..202 e-value: 8.3E-12 score: 46.7
None	No IPR available	PANTHER	PTHR31490:SF52	ENDO-1,4-BETA-XYLANASE 5-RELATED	coord: 50..164
IPR044846	Glycoside hydrolase family 10	PANTHER	PTHR31490	GLYCOSYL HYDROLASE	coord: 50..164
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 59..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G005810.1	Lsi03G005810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds