Lsi03G003670 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G003670
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionE3 ubiquitin-protein ligase SDIR1
Locationchr03: 4314295 .. 4317810 (+)
RNA-Seq ExpressionLsi03G003670
SyntenyLsi03G003670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACTTGAGTTTGAGTGCACTCATCTTACAGTCCTTTGGTTCCATTGGATGCGCAAATGATGGGAAAATGTCTGGTGTACTTCTGGATGTCTTTTCACTTGTCGGACAATGCAGTTCCTTTCCTTGAGGGTTTTCAGGATTAATATATTGTTGTCTGTGTTGTCTGAAGGAATCAGTTAATTTTAGTTATCTAATCTTTATGAAGCAGGTGATCAGATCACAAATTAGGTTTAGGCATTGAATTTGACAACTAAGGAATAATTAATTGGGAATTCTATGCTCTTCTTGTTGAATTGCACATGTACTGGATGTAGCGTATAGGAACATGTCAATACTTGCAAGAACTTCAAACTCGTTGAAATTATCTATGATTATATTTTTGTTATATGAGTGATTCATTGTTTAGCCATGGACATTACATCTGTGCATTTATATGGTTAAAGGAATGTCAAAGTTTAGGCTATTATGAGTCTTTGATTCTTTGAACTTCACTTCTTATGTTATTCATTCGTTGAACAATCCATCGGGGCTGAGAATGATTATGGGTTAGTGTAGTAAGGAGATACGGGAATTGTTTTATTTATACTGGAGCCTCTTTCTCTCTGAGTTATTTAGATTATTACCATAAGTTCATATGACTTTCATTCTGTAGATTCACATTACTCCGAAATCAACTAGCTGTTGCAACTATTGCAGACATGAGTTTTGTTTTCCGTGGAACGAGAGTTCCAGACATTGAAAATGGTCTATCTGGATTTATACCCGAACGGCGTGCAATGGTATGCTCAATTTTTCGTACTCTTGACTTGATAATACTAATGCTTGGAAATTTGTAGATATTGCACTAGTTATGATCTAATTTAAAAAATATGTTTTTTTCTGCTCACAATCAGAGGGTTCATGCAGCACGTCCCGTTAACTCAAACTCACTTGCCTTTCTCGTCACAGGTACCATTTCACCTATGTGCAGGCTGATATCTGTTTCATGTGAGAATTTTGATCTACTGCATTGTTTTAATTCCTTGACTTTGTTGCAGTTCTTTTGTTATTCATGATATTAAATTCTCACCAGATGTCACCGAACTTTCTGGTGAGTTTCATGTTCCAAACTGTGAATGAGTAGTAGGTTGGTCTTACTCTCTCACCATTGGTGCGAATCTTTACATTTTCTTCCTTTGAACATGCATATTTAGCTCTGGCTTGTGCTTGGTGTCTTTTTGATGGCTACAACATTAAGGATGTACGCAACTTGTCAACAACTTCAGGCTCAAGCCCAGGCTAGAGCTATGGCAGCCAGTGGCCTTCTTGGTCACACTGAATTGAGGTTACATATGCCTCCATCAGTAGCACTTGCAACTAGAGGGCGTTTACAAGGGTTAAGGCTTCAACTTGCTCTACTGGATCGGGAATTCGATGATCTAGGTATGGTTCAAATCTAAAAAGTGCAAGTTCGTAGCTTCAAACTTAGTCAAACGTGGCCACCATTCTTTTACATTATTCTAGTGTACCGCTTTCACCTACTTTCAGCAACATTTTTCAGTGGCACAAACTCTCTCATGTGTGTGTGCGTACGTGTGGGGGGAATTTGAGACTGAAATATCCACTTTGTGGCAAAATTTGGGGAAACTTTATCAATCGGATTTCTTTGACAATATTCTGAAGTGTAATCATTTTGATGTGGGTTTCTGAAAATGCAAGAAATTCACTCCTTTCATTGCCAAATTTGGGAAATTTTTTGTTGATCAGACTTCTTTGGCAAGGTTCTGACAGGATGATAAGCTTGCGGTAAGTGCATGAAGTCAAATAGGATAGAAGTGGTGCATTTCATGTTGTCAATTGTTGAAAAGAAGTACCCTGAACTTGCTTCTGACAGTATGGTTGGGATGGACAGTACCTCATGAAACTTTAAATTTGTTGGGTTTCTTTTTCTTATTTTAAACTTTTTTTTAAAGCTCATCATCATTGTCTTTTAAGTTTCATATGTATAATCAATGATGCGGTAATAAACATGTCGTTTATCTTCAGATTATGAAACTTTGAGAGCTTTGGATTCTGATAATGCTCCGACGACACCTTCTATGAGTGAGGAACAAATAAATGCTCTTCCCGTTCATAAGTACAAGGTTTCTGGTCCTCAAAGGTAATCAACCCCATATTAGTCTACATTCCAGAATGAATTAGGGTATCTGTTGCTTTACTTTGAGTTTTGATTGGAAGCCACATATTCTGCACTGAGATTTTTCAACGATGAAATATGCGTACTCCAGCTTGTCACACAAAAACTAAAATTAAGTTTTCATTAAATTATCTACAGTGACCCCTCTGTGAATCAGCAGGCTTCATCTTCAGAATCTAATGAGGTATCGATTAGGTTACCTTTTCCTTCCTTGTTTGATTCTTTTATTGCCTTAAACGGTGTGTACAAAATTACAAATACAATAATGACTCGTTTCAGAAGGAAATATAGTGCAGAGAAGGGACAGAAGGCCAAAATTTTCCTTTTTTTAGCTTATTATTTGTGTACCACACACTCTTTCTCATCATTTATCAATTTTCAAGAACGTACTGTTTTTTTTTAATTTTGATTGCAGAAGAGACAAGATTCAGTTAATGCAGTTGGCGGTACCAAGACCTCTGAGGATGAACTCACATGCAGTGTTTGCTTGGAGCAAGTAAATGTTGGTGAACTCATACGTAGTTTGCCATGTTTGCACCAGGTTAATCTCTTATCTCTTTGCGGCCTTTCCTTCCAAACAAATGGTTACTAAATATTTCATATTGAAAATTCAGAAATTTGGTTTTTTGTTATTATTATTACTTGTTGTGGTCGTTTTGTTTTGTTTGTCTTCTTCGAAGGCCTCTTGCACAGCCCCTCTTTCCTAAATTTTGATACACCTTCTAACTGCTGGCATTTCAACGGTTCTTTTAAATTGAATGTGGTTATACATGAATTCAATCTTGTAGTTCCATGCCAACTGCATAGATCCATGGCTGCGACAGCAGGGAACGTGCCCTGTTTGTAAATTCCGAGCGGTGTCCGGGTGGTCGGAACAGGGGCAAGGGGAAACCGATGCTTATATGGTTTAATTGGATGCAAAGCAAGTTATTGAACAACGCTTGCTTGACGCCTGATTAAAGGGCAGAGGATATGGACAGACGTGGTGTAATTAGGTTAAGGGTAGGCATTACAGGAATTGCAGTTGCCGTTCTGTATCTTTGAACTTCTGACGAGCCATTTAATTGTATTCTTGAAGTTCATCATTTGTGATATATAGTCTCTGACCATTGTGAGTGAGCAGAATTTTGGTTCTTTCACTGTCTTCAATGTATTATTCCAACCCTTACTAGCAAGTACTTGTTACCTCTAGAAATAAACACCACAATGAACATTTTCCACAATTCTCCATTGGCTTATTTGTAGTGTAGTATTGTAATAAGAAGACGGGAAAAAGGGTAATTTTATTCTAAGCTATCAGTGAAATTAAAAGTAAAATGCCAACTTAATATCCAGCAT

mRNA sequence

ATGCACTTGAGTTTGAGTGCACTCATCTTACAGTCCTTTGGTTCCATTGGATGCGCAAATGATGGGAAAATATTCACATTACTCCGAAATCAACTAGCTGTTGCAACTATTGCAGACATGAGTTTTGTTTTCCGTGGAACGAGAGTTCCAGACATTGAAAATGGTCTATCTGGATTTATACCCGAACGGCGTGCAATGAGGGTTCATGCAGCACGTCCCGTTAACTCAAACTCACTTGCCTTTCTCGTCACAGTTCTTTTGTTATTCATGATATTAAATTCTCACCAGATGTCACCGAACTTTCTGCTCTGGCTTGTGCTTGGTGTCTTTTTGATGGCTACAACATTAAGGATGTACGCAACTTGTCAACAACTTCAGGCTCAAGCCCAGGCTAGAGCTATGGCAGCCAGTGGCCTTCTTGGTCACACTGAATTGAGGTTACATATGCCTCCATCAGTAGCACTTGCAACTAGAGGGCGTTTACAAGGGTTAAGGCTTCAACTTGCTCTACTGGATCGGGAATTCGATGATCTAGATTATGAAACTTTGAGAGCTTTGGATTCTGATAATGCTCCGACGACACCTTCTATGAGTGAGGAACAAATAAATGCTCTTCCCGTTCATAAGTACAAGGTTTCTGGTCCTCAAAGTGACCCCTCTGTGAATCAGCAGGCTTCATCTTCAGAATCTAATGAGAAGAGACAAGATTCAGTTAATGCAGTTGGCGGTACCAAGACCTCTGAGGATGAACTCACATGCAGTGTTTGCTTGGAGCAAGTAAATGTTGGTGAACTCATACGTAGTTTGCCATGTTTGCACCAGTTCCATGCCAACTGCATAGATCCATGGCTGCGACAGCAGGGAACGTGCCCTGTTTGTAAATTCCGAGCGGTGTCCGGGTGGTCGGAACAGGGGCAAGGGGAAACCGATGCTTATATGGTTTAATTGGATGCAAAGCAAGTTATTGAACAACGCTTGCTTGACGCCTGATTAAAGGGCAGAGGATATGGACAGACGTGGTGTAATTAGGTTAAGGGTAGGCATTACAGGAATTGCAGTTGCCGTTCTGTATCTTTGAACTTCTGACGAGCCATTTAATTGTATTCTTGAAGTTCATCATTTGTGATATATAGTCTCTGACCATTGTGAGTGAGCAGAATTTTGGTTCTTTCACTGTCTTCAATGTATTATTCCAACCCTTACTAGCAAGTACTTGTTACCTCTAGAAATAAACACCACAATGAACATTTTCCACAATTCTCCATTGGCTTATTTGTAGTGTAGTATTGTAATAAGAAGACGGGAAAAAGGGTAATTTTATTCTAAGCTATCAGTGAAATTAAAAGTAAAATGCCAACTTAATATCCAGCAT

Coding sequence (CDS)

ATGCACTTGAGTTTGAGTGCACTCATCTTACAGTCCTTTGGTTCCATTGGATGCGCAAATGATGGGAAAATATTCACATTACTCCGAAATCAACTAGCTGTTGCAACTATTGCAGACATGAGTTTTGTTTTCCGTGGAACGAGAGTTCCAGACATTGAAAATGGTCTATCTGGATTTATACCCGAACGGCGTGCAATGAGGGTTCATGCAGCACGTCCCGTTAACTCAAACTCACTTGCCTTTCTCGTCACAGTTCTTTTGTTATTCATGATATTAAATTCTCACCAGATGTCACCGAACTTTCTGCTCTGGCTTGTGCTTGGTGTCTTTTTGATGGCTACAACATTAAGGATGTACGCAACTTGTCAACAACTTCAGGCTCAAGCCCAGGCTAGAGCTATGGCAGCCAGTGGCCTTCTTGGTCACACTGAATTGAGGTTACATATGCCTCCATCAGTAGCACTTGCAACTAGAGGGCGTTTACAAGGGTTAAGGCTTCAACTTGCTCTACTGGATCGGGAATTCGATGATCTAGATTATGAAACTTTGAGAGCTTTGGATTCTGATAATGCTCCGACGACACCTTCTATGAGTGAGGAACAAATAAATGCTCTTCCCGTTCATAAGTACAAGGTTTCTGGTCCTCAAAGTGACCCCTCTGTGAATCAGCAGGCTTCATCTTCAGAATCTAATGAGAAGAGACAAGATTCAGTTAATGCAGTTGGCGGTACCAAGACCTCTGAGGATGAACTCACATGCAGTGTTTGCTTGGAGCAAGTAAATGTTGGTGAACTCATACGTAGTTTGCCATGTTTGCACCAGTTCCATGCCAACTGCATAGATCCATGGCTGCGACAGCAGGGAACGTGCCCTGTTTGTAAATTCCGAGCGGTGTCCGGGTGGTCGGAACAGGGGCAAGGGGAAACCGATGCTTATATGGTTTAA

Protein sequence

MHLSLSALILQSFGSIGCANDGKIFTLLRNQLAVATIADMSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSPNFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATRGRLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDPSVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANCIDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV
Homology
BLAST of Lsi03G003670 vs. ExPASy Swiss-Prot
Match: Q9M2S6 (E3 ubiquitin-protein ligase SDIR1 OS=Arabidopsis thaliana OX=3702 GN=SDIR1 PE=1 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 2.7e-113
Identity = 218/273 (79.85%), Postives = 237/273 (86.81%), Query Frame = 0

Query: 40  MSFVFRGTRVPDIENGLS-GFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMS 99
           MSFVFRG+R  D+E+G S GF+PERRAMRVH ARPVNSNSLAFLVTVLLLFMILNSHQM 
Sbjct: 1   MSFVFRGSR-GDLESGFSGGFLPERRAMRVHGARPVNSNSLAFLVTVLLLFMILNSHQMP 60

Query: 100 PNFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATR 159
           PNFLLWLVLGVFLMATTLRMYATCQQLQA AQA+A AASGL  HTELRLH+PPS+ALATR
Sbjct: 61  PNFLLWLVLGVFLMATTLRMYATCQQLQAHAQAQAAAASGLFSHTELRLHVPPSIALATR 120

Query: 160 GRLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSD 219
           GRLQGLRLQLALLDREFDDLDYETLRALDSDN  TT SMSEE+INALPVHKYKV  P++ 
Sbjct: 121 GRLQGLRLQLALLDREFDDLDYETLRALDSDNVSTT-SMSEEEINALPVHKYKVLDPENG 180

Query: 220 PSVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHAN 279
            S+ +QAS+S S EK  DS N     K +EDELTCSVCLEQV VGE++R+LPCLHQFHA 
Sbjct: 181 CSLAKQASTSSSAEKMLDSANE--SKKGTEDELTCSVCLEQVTVGEIVRTLPCLHQFHAG 240

Query: 280 CIDPWLRQQGTCPVCKFRAVSGWSEQGQGETDA 312
           CIDPWLRQQGTCPVCKFRA SGW EQ + + DA
Sbjct: 241 CIDPWLRQQGTCPVCKFRAHSGWQEQDEIDDDA 269

BLAST of Lsi03G003670 vs. ExPASy Swiss-Prot
Match: Q68DV7 (E3 ubiquitin-protein ligase RNF43 OS=Homo sapiens OX=9606 GN=RNF43 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-11
Identity = 28/61 (45.90%), Postives = 40/61 (65.57%), Query Frame = 0

Query: 253 CSVCLEQVNVGELIRSLPCLHQFHANCIDPWLRQQGTCPVCKFRAVSGWS-EQGQGETDA 312
           C++CLE+ + G+ +R + CLH+FH NC+DPWL Q  TCP+C F    G S  Q  G + +
Sbjct: 272 CAICLEEFSEGQELRVISCLHEFHRNCVDPWLHQHRTCPLCMFNITEGDSFSQSLGPSRS 331

BLAST of Lsi03G003670 vs. ExPASy Swiss-Prot
Match: Q10R93 (Receptor homology region, transmembrane domain- and RING domain-containing protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0167500 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 6.6e-11
Identity = 35/70 (50.00%), Postives = 42/70 (60.00%), Query Frame = 0

Query: 244 TKTSEDELT---CSVCLEQVNVGELIRSLPCLHQFHANCIDPWLRQQGT-CPVCKFRAVS 303
           TK  ED  T   C++CLE  NVGE +R LPC H+FHA C+D WL    T CPVCK  A +
Sbjct: 224 TKVQEDNCTSSMCAICLEDYNVGEKLRVLPCRHKFHAACVDLWLTTWRTFCPVCKRDAST 283

Query: 304 GWSEQGQGET 310
           G  +    ET
Sbjct: 284 GIPDPPASET 293

BLAST of Lsi03G003670 vs. ExPASy Swiss-Prot
Match: Q5NCP0 (E3 ubiquitin-protein ligase RNF43 OS=Mus musculus OX=10090 GN=Rnf43 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 6.6e-11
Identity = 24/48 (50.00%), Postives = 34/48 (70.83%), Query Frame = 0

Query: 253 CSVCLEQVNVGELIRSLPCLHQFHANCIDPWLRQQGTCPVCKFRAVSG 301
           C++CLE+ + G+ +R + CLH+FH  C+DPWL Q  TCP+C F  V G
Sbjct: 272 CAICLEEFSEGQELRVISCLHEFHRTCVDPWLYQHRTCPLCMFNIVEG 319

BLAST of Lsi03G003670 vs. ExPASy Swiss-Prot
Match: Q8NC42 (E3 ubiquitin-protein ligase RNF149 OS=Homo sapiens OX=9606 GN=RNF149 PE=2 SV=2)

HSP 1 Score: 68.2 bits (165), Expect = 1.9e-10
Identity = 29/60 (48.33%), Postives = 36/60 (60.00%), Query Frame = 0

Query: 249 DELTCSVCLEQVNVGELIRSLPCLHQFHANCIDPWLRQQGTCPVCK---FRAVSGWSEQG 306
           D   C+VC+E   V ++IR LPC H FH  CIDPWL    TCP+CK    +A+  W E G
Sbjct: 265 DAENCAVCIENFKVKDIIRILPCKHIFHRICIDPWLLDHRTCPMCKLDVIKALGYWGEPG 324

BLAST of Lsi03G003670 vs. ExPASy TrEMBL
Match: E5GC70 (Protein binding protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 6.0e-148
Identity = 272/275 (98.91%), Postives = 273/275 (99.27%), Query Frame = 0

Query: 40  MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 99
           MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP
Sbjct: 1   MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 60

Query: 100 NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATRG 159
           NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPS+ALATRG
Sbjct: 61  NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSIALATRG 120

Query: 160 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 219
           RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSD 
Sbjct: 121 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDS 180

Query: 220 SVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 279
           SVNQQASSSESNEKRQDSVNAVG TKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC
Sbjct: 181 SVNQQASSSESNEKRQDSVNAVGSTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 240

Query: 280 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 315
           IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV
Sbjct: 241 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 275

BLAST of Lsi03G003670 vs. ExPASy TrEMBL
Match: A0A1S3B3A3 (E3 ubiquitin-protein ligase SDIR1 OS=Cucumis melo OX=3656 GN=LOC103485511 PE=4 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 6.0e-148
Identity = 272/275 (98.91%), Postives = 273/275 (99.27%), Query Frame = 0

Query: 40  MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 99
           MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP
Sbjct: 1   MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 60

Query: 100 NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATRG 159
           NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPS+ALATRG
Sbjct: 61  NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSIALATRG 120

Query: 160 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 219
           RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSD 
Sbjct: 121 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDS 180

Query: 220 SVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 279
           SVNQQASSSESNEKRQDSVNAVG TKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC
Sbjct: 181 SVNQQASSSESNEKRQDSVNAVGSTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 240

Query: 280 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 315
           IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV
Sbjct: 241 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 275

BLAST of Lsi03G003670 vs. ExPASy TrEMBL
Match: A0A6J1FER7 (E3 ubiquitin-protein ligase SDIR1 OS=Cucurbita moschata OX=3662 GN=LOC111444825 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.1e-146
Identity = 268/275 (97.45%), Postives = 271/275 (98.55%), Query Frame = 0

Query: 40  MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 99
           MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFM+LNSHQMSP
Sbjct: 1   MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMMLNSHQMSP 60

Query: 100 NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATRG 159
           NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPS+ALATRG
Sbjct: 61  NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSIALATRG 120

Query: 160 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 219
           RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP
Sbjct: 121 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 180

Query: 220 SVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 279
           SVNQQASSSESNEKRQDS NAVG TK SEDELTCSVCLEQVNVGEL+RSLPCLHQFHANC
Sbjct: 181 SVNQQASSSESNEKRQDSANAVGSTKASEDELTCSVCLEQVNVGELVRSLPCLHQFHANC 240

Query: 280 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 315
           IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAY V
Sbjct: 241 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYSV 275

BLAST of Lsi03G003670 vs. ExPASy TrEMBL
Match: A0A0A0KBF9 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G008630 PE=4 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 5.6e-146
Identity = 270/275 (98.18%), Postives = 271/275 (98.55%), Query Frame = 0

Query: 40  MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 99
           MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP
Sbjct: 126 MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 185

Query: 100 NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATRG 159
           NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPS+ALATRG
Sbjct: 186 NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSIALATRG 245

Query: 160 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 219
           RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSD 
Sbjct: 246 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDS 305

Query: 220 SVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 279
           SVNQQASSSES EKRQDSVNAVG TKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC
Sbjct: 306 SVNQQASSSESIEKRQDSVNAVGSTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 365

Query: 280 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 315
           IDPWLRQQGTCPVCKFRAVSGWSEQ QGETDAYMV
Sbjct: 366 IDPWLRQQGTCPVCKFRAVSGWSEQEQGETDAYMV 400

BLAST of Lsi03G003670 vs. ExPASy TrEMBL
Match: A0A6J1JZS1 (E3 ubiquitin-protein ligase SDIR1-like OS=Cucurbita maxima OX=3661 GN=LOC111488912 PE=4 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 9.6e-146
Identity = 266/275 (96.73%), Postives = 270/275 (98.18%), Query Frame = 0

Query: 40  MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 99
           MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSL FLVTVLLLFM+LNSHQMSP
Sbjct: 1   MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLVFLVTVLLLFMMLNSHQMSP 60

Query: 100 NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATRG 159
           NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPS+ALATRG
Sbjct: 61  NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSIALATRG 120

Query: 160 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 219
           RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP
Sbjct: 121 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 180

Query: 220 SVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 279
           SVNQQASSSESNEKRQDS NAVG TK SEDELTCSVCLEQVNVGEL+RSLPCLHQFHANC
Sbjct: 181 SVNQQASSSESNEKRQDSANAVGSTKASEDELTCSVCLEQVNVGELVRSLPCLHQFHANC 240

Query: 280 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 315
           IDPWL+QQGTCPVCKFRAVSGWSEQGQGETDAY V
Sbjct: 241 IDPWLQQQGTCPVCKFRAVSGWSEQGQGETDAYSV 275

BLAST of Lsi03G003670 vs. NCBI nr
Match: XP_008441380.1 (PREDICTED: E3 ubiquitin-protein ligase SDIR1 [Cucumis melo] >ADN34069.1 protein binding protein [Cucumis melo subsp. melo])

HSP 1 Score: 533.5 bits (1373), Expect = 1.2e-147
Identity = 272/275 (98.91%), Postives = 273/275 (99.27%), Query Frame = 0

Query: 40  MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 99
           MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP
Sbjct: 1   MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 60

Query: 100 NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATRG 159
           NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPS+ALATRG
Sbjct: 61  NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSIALATRG 120

Query: 160 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 219
           RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSD 
Sbjct: 121 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDS 180

Query: 220 SVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 279
           SVNQQASSSESNEKRQDSVNAVG TKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC
Sbjct: 181 SVNQQASSSESNEKRQDSVNAVGSTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 240

Query: 280 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 315
           IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV
Sbjct: 241 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 275

BLAST of Lsi03G003670 vs. NCBI nr
Match: XP_038885371.1 (E3 ubiquitin-protein ligase SDIR1 isoform X1 [Benincasa hispida] >XP_038885372.1 E3 ubiquitin-protein ligase SDIR1 isoform X1 [Benincasa hispida] >XP_038885373.1 E3 ubiquitin-protein ligase SDIR1 isoform X1 [Benincasa hispida] >XP_038885374.1 E3 ubiquitin-protein ligase SDIR1 isoform X1 [Benincasa hispida])

HSP 1 Score: 532.3 bits (1370), Expect = 2.8e-147
Identity = 271/275 (98.55%), Postives = 272/275 (98.91%), Query Frame = 0

Query: 40  MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 99
           MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP
Sbjct: 1   MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 60

Query: 100 NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATRG 159
           NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPS+ALATRG
Sbjct: 61  NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSIALATRG 120

Query: 160 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 219
           RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKV GPQSDP
Sbjct: 121 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVPGPQSDP 180

Query: 220 SVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 279
           SVNQQASSSESNEKRQD VNAVG TKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC
Sbjct: 181 SVNQQASSSESNEKRQDLVNAVGSTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 240

Query: 280 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 315
           IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV
Sbjct: 241 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 275

BLAST of Lsi03G003670 vs. NCBI nr
Match: XP_023551037.1 (E3 ubiquitin-protein ligase SDIR1 [Cucurbita pepo subsp. pepo] >XP_023551039.1 E3 ubiquitin-protein ligase SDIR1 [Cucurbita pepo subsp. pepo] >XP_023551040.1 E3 ubiquitin-protein ligase SDIR1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 531.2 bits (1367), Expect = 6.1e-147
Identity = 269/275 (97.82%), Postives = 272/275 (98.91%), Query Frame = 0

Query: 40  MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 99
           MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFM+LNSHQMSP
Sbjct: 1   MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMMLNSHQMSP 60

Query: 100 NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATRG 159
           NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPS+ALATRG
Sbjct: 61  NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSIALATRG 120

Query: 160 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 219
           RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP
Sbjct: 121 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 180

Query: 220 SVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 279
           SVNQQASSSESNEKRQDS NAVG TKTSEDELTCSVCLEQVNVGEL+RSLPCLHQFHANC
Sbjct: 181 SVNQQASSSESNEKRQDSANAVGSTKTSEDELTCSVCLEQVNVGELVRSLPCLHQFHANC 240

Query: 280 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 315
           IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAY V
Sbjct: 241 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYSV 275

BLAST of Lsi03G003670 vs. NCBI nr
Match: XP_022938667.1 (E3 ubiquitin-protein ligase SDIR1 [Cucurbita moschata] >XP_022938669.1 E3 ubiquitin-protein ligase SDIR1 [Cucurbita moschata] >XP_022938670.1 E3 ubiquitin-protein ligase SDIR1 [Cucurbita moschata] >KAG6578411.1 E3 ubiquitin-protein ligase SDIR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 529.3 bits (1362), Expect = 2.3e-146
Identity = 268/275 (97.45%), Postives = 271/275 (98.55%), Query Frame = 0

Query: 40  MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 99
           MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFM+LNSHQMSP
Sbjct: 1   MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMMLNSHQMSP 60

Query: 100 NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATRG 159
           NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPS+ALATRG
Sbjct: 61  NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSIALATRG 120

Query: 160 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 219
           RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP
Sbjct: 121 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 180

Query: 220 SVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 279
           SVNQQASSSESNEKRQDS NAVG TK SEDELTCSVCLEQVNVGEL+RSLPCLHQFHANC
Sbjct: 181 SVNQQASSSESNEKRQDSANAVGSTKASEDELTCSVCLEQVNVGELVRSLPCLHQFHANC 240

Query: 280 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYMV 315
           IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAY V
Sbjct: 241 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAYSV 275

BLAST of Lsi03G003670 vs. NCBI nr
Match: KAG7015976.1 (E3 ubiquitin-protein ligase SDIR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 527.7 bits (1358), Expect = 6.8e-146
Identity = 267/273 (97.80%), Postives = 270/273 (98.90%), Query Frame = 0

Query: 40  MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMSP 99
           MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFM+LNSHQMSP
Sbjct: 1   MSFVFRGTRVPDIENGLSGFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMMLNSHQMSP 60

Query: 100 NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATRG 159
           NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPS+ALATRG
Sbjct: 61  NFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSIALATRG 120

Query: 160 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 219
           RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP
Sbjct: 121 RLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDP 180

Query: 220 SVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANC 279
           SVNQQASSSESNEKRQDS NAVG TK SEDELTCSVCLEQVNVGEL+RSLPCLHQFHANC
Sbjct: 181 SVNQQASSSESNEKRQDSANAVGSTKASEDELTCSVCLEQVNVGELVRSLPCLHQFHANC 240

Query: 280 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAY 313
           IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAY
Sbjct: 241 IDPWLRQQGTCPVCKFRAVSGWSEQGQGETDAY 273

BLAST of Lsi03G003670 vs. TAIR 10
Match: AT3G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 409.8 bits (1052), Expect = 1.9e-114
Identity = 218/273 (79.85%), Postives = 237/273 (86.81%), Query Frame = 0

Query: 40  MSFVFRGTRVPDIENGLS-GFIPERRAMRVHAARPVNSNSLAFLVTVLLLFMILNSHQMS 99
           MSFVFRG+R  D+E+G S GF+PERRAMRVH ARPVNSNSLAFLVTVLLLFMILNSHQM 
Sbjct: 1   MSFVFRGSR-GDLESGFSGGFLPERRAMRVHGARPVNSNSLAFLVTVLLLFMILNSHQMP 60

Query: 100 PNFLLWLVLGVFLMATTLRMYATCQQLQAQAQARAMAASGLLGHTELRLHMPPSVALATR 159
           PNFLLWLVLGVFLMATTLRMYATCQQLQA AQA+A AASGL  HTELRLH+PPS+ALATR
Sbjct: 61  PNFLLWLVLGVFLMATTLRMYATCQQLQAHAQAQAAAASGLFSHTELRLHVPPSIALATR 120

Query: 160 GRLQGLRLQLALLDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSD 219
           GRLQGLRLQLALLDREFDDLDYETLRALDSDN  TT SMSEE+INALPVHKYKV  P++ 
Sbjct: 121 GRLQGLRLQLALLDREFDDLDYETLRALDSDNVSTT-SMSEEEINALPVHKYKVLDPENG 180

Query: 220 PSVNQQASSSESNEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHAN 279
            S+ +QAS+S S EK  DS N     K +EDELTCSVCLEQV VGE++R+LPCLHQFHA 
Sbjct: 181 CSLAKQASTSSSAEKMLDSANE--SKKGTEDELTCSVCLEQVTVGEIVRTLPCLHQFHAG 240

Query: 280 CIDPWLRQQGTCPVCKFRAVSGWSEQGQGETDA 312
           CIDPWLRQQGTCPVCKFRA SGW EQ + + DA
Sbjct: 241 CIDPWLRQQGTCPVCKFRAHSGWQEQDEIDDDA 269

BLAST of Lsi03G003670 vs. TAIR 10
Match: AT1G04790.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 7.3e-13
Identity = 43/124 (34.68%), Postives = 60/124 (48.39%), Query Frame = 0

Query: 171 LDREFDDLDYETLRALDSDNAPTTPSMSEEQINALPVHKYKVSGPQSDPSVNQQASSSES 230
           +DR+F + DYE L ALD +N                 H++   G  S   +N    S+  
Sbjct: 542 MDRDFTEDDYELLLALDENN-----------------HRH---GGASANRINNLPESTVQ 601

Query: 231 NEKRQDSVNAVGGTKTSEDELTCSVCLEQVNVGELIRSLPCLHQFHANCIDPWLRQQGTC 290
            +  Q+               TC +CLE   +G+ IR LPCLH+FH +CIDPWL +  +C
Sbjct: 602 TDNFQE---------------TCVICLETPKIGDTIRHLPCLHKFHKDCIDPWLGRSKSC 630

Query: 291 PVCK 295
           PVCK
Sbjct: 662 PVCK 630

BLAST of Lsi03G003670 vs. TAIR 10
Match: AT5G66070.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 2.3e-11
Identity = 31/63 (49.21%), Postives = 42/63 (66.67%), Query Frame = 0

Query: 236 DSVNAVGG---TKTSEDELTCSVCLEQVNVGELIRSLP-CLHQFHANCIDPWLRQQGTCP 295
           DS+N +     T TS + ++CSVCL+   VGE +RSLP C H FH  CID WLR+  +CP
Sbjct: 156 DSLNRIPKVRITDTSPEIVSCSVCLQDFQVGETVRSLPHCHHMFHLPCIDKWLRRHASCP 215

BLAST of Lsi03G003670 vs. TAIR 10
Match: AT5G66070.2 (RING/U-box superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 2.3e-11
Identity = 31/63 (49.21%), Postives = 42/63 (66.67%), Query Frame = 0

Query: 236 DSVNAVGG---TKTSEDELTCSVCLEQVNVGELIRSLP-CLHQFHANCIDPWLRQQGTCP 295
           DS+N +     T TS + ++CSVCL+   VGE +RSLP C H FH  CID WLR+  +CP
Sbjct: 180 DSLNRIPKVRITDTSPEIVSCSVCLQDFQVGETVRSLPHCHHMFHLPCIDKWLRRHASCP 239

BLAST of Lsi03G003670 vs. TAIR 10
Match: AT1G80400.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 3.1e-11
Identity = 35/109 (32.11%), Postives = 57/109 (52.29%), Query Frame = 0

Query: 189 DNAPTTPSMSEEQINALPVHKYKVSGPQSDPSVNQQASSSESNEKRQDSVNAVGGTK--- 248
           +N   T   + E INALPV+++K          ++  +  E +E+ +     +G  K   
Sbjct: 298 ENFSQTRGATAEAINALPVYRFK----------SKSRNDLEFSEEGEGGFLLLGSQKKRL 357

Query: 249 TSEDELTCSVCLEQVNVGELIRSLPCLHQFHANCIDPWLRQQGTCPVCK 295
            S ++ +C +CL +    E +R LPC H FH +C+D WL+   TCP+CK
Sbjct: 358 ISGEDASCCICLTRYGDDEQVRELPCSHVFHVDCVDKWLKINATCPLCK 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2S62.7e-11379.85E3 ubiquitin-protein ligase SDIR1 OS=Arabidopsis thaliana OX=3702 GN=SDIR1 PE=1 ... [more]
Q68DV71.0e-1145.90E3 ubiquitin-protein ligase RNF43 OS=Homo sapiens OX=9606 GN=RNF43 PE=1 SV=1[more]
Q10R936.6e-1150.00Receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
Q5NCP06.6e-1150.00E3 ubiquitin-protein ligase RNF43 OS=Mus musculus OX=10090 GN=Rnf43 PE=1 SV=1[more]
Q8NC421.9e-1048.33E3 ubiquitin-protein ligase RNF149 OS=Homo sapiens OX=9606 GN=RNF149 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
E5GC706.0e-14898.91Protein binding protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3B3A36.0e-14898.91E3 ubiquitin-protein ligase SDIR1 OS=Cucumis melo OX=3656 GN=LOC103485511 PE=4 S... [more]
A0A6J1FER71.1e-14697.45E3 ubiquitin-protein ligase SDIR1 OS=Cucurbita moschata OX=3662 GN=LOC111444825 ... [more]
A0A0A0KBF95.6e-14698.18RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G008630 P... [more]
A0A6J1JZS19.6e-14696.73E3 ubiquitin-protein ligase SDIR1-like OS=Cucurbita maxima OX=3661 GN=LOC1114889... [more]
Match NameE-valueIdentityDescription
XP_008441380.11.2e-14798.91PREDICTED: E3 ubiquitin-protein ligase SDIR1 [Cucumis melo] >ADN34069.1 protein ... [more]
XP_038885371.12.8e-14798.55E3 ubiquitin-protein ligase SDIR1 isoform X1 [Benincasa hispida] >XP_038885372.1... [more]
XP_023551037.16.1e-14797.82E3 ubiquitin-protein ligase SDIR1 [Cucurbita pepo subsp. pepo] >XP_023551039.1 E... [more]
XP_022938667.12.3e-14697.45E3 ubiquitin-protein ligase SDIR1 [Cucurbita moschata] >XP_022938669.1 E3 ubiqui... [more]
KAG7015976.16.8e-14697.80E3 ubiquitin-protein ligase SDIR1, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT3G55530.11.9e-11479.85RING/U-box superfamily protein [more]
AT1G04790.17.3e-1334.68RING/U-box superfamily protein [more]
AT5G66070.12.3e-1149.21RING/U-box superfamily protein [more]
AT5G66070.22.3e-1149.21RING/U-box superfamily protein [more]
AT1G80400.13.1e-1132.11RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 253..293
e-value: 1.7E-8
score: 44.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 252..294
e-value: 3.7E-12
score: 46.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 253..294
score: 12.282736
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 214..298
e-value: 1.0E-19
score: 72.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 210..244
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 215..244
NoneNo IPR availablePANTHERPTHR45977:SF4E3 UBIQUITIN-PROTEIN LIGASE SDIR1coord: 40..313
NoneNo IPR availablePANTHERPTHR45977TARGET OF ERK KINASE MPK-1coord: 40..313
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 252..294
e-value: 3.28801E-24
score: 90.8241
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 247..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G003670.1Lsi03G003670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009789 positive regulation of abscisic acid-activated signaling pathway
biological_process GO:0016567 protein ubiquitination
biological_process GO:0009651 response to salt stress
biological_process GO:0009414 response to water deprivation
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity