Lsi03G002190 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G002190
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionEndo-1,3(4)-beta-glucanase
Locationchr03: 2770217 .. 2772418 (+)
RNA-Seq ExpressionLsi03G002190
SyntenyLsi03G002190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCAAGAAGATTGAAAGAGAGCTCAGGCGGGTAATCAAAGGGAAACGTCGCTCCCCTCCACCGCGTCTTCCACCACCACCTCCTCCTCCTCCTTCACCTCCACCTCCACCTCCACCACAGCCCCCCGAGTCACCACCACCAGAACCTTCTCAAAAGCCATCAGAGCCTACTCCATTCCTATTTCCTTCAACTCAATCCACAGTTCTCCCGGATCCTTCACTATTCTTTTCCCCTGAACTCCTCTCAGCCCCTCTCCCTACAAACAGTTTCTTCCAGAACCTCACTCTCAATAATGGCGACCAACCTGAGTACATCCATCCCTACTTGATCAAATCCTCCCTCTCCTCCATCTCTGTTTCCTACCCATCAATCTCCTCCAACTCTACTTCGGAATGCCAAATTTTCACCCCTGACCTTACCATCTCTTCCTCTGAAAAAATAAACCCACTTCCCCAGAAATCTCATGTAATCTCCTCATTCAATGATTTGAATGTCACTTTGGATATTCCTTCCTCCAACCTCCGTTTCTATCTCGTCCGGGGAAGCCCATTTCTGACTTTCACAGTTTCCAAAGGCGGAGCCTTTTCAATCTCCACCATTCACGAAGTTATCTCCTTTTCTTTTAACAATGCACTCACCAAATACACAATAAAATTGAAAAACAATCAAACATGGCTTATTTACTCCTCGTTTCCAATCAATTTGAGGCATAATCTTTCTATGATTACTTCTGGGGCATTTGCAGGAATCATTAGGATTGCAGCATTGCCAAATTTAGATCCGGAATGTGAACTGATACTTGACCGGTTTAGTTCGTGTTTCCCAGTCTCGGGTGAGGCACAGTTTACGAAGCCATTTTGTTTGGAATACAAATGGGAGACAAAAGGGTGGGGGGATTTGCTCATGCTTGCACACCCTCTGCATCTTCATCTCCTAGCAGGCACTGATAATGATGTAGTTATTTTAGATAAGCTCAAGTATAAAAGTATAGATGGTGAGCTTGTGGGTATGGTGGGAAGTTCATGGGCTTTGAAACCAGAACCTATTTCAGTAAGCTGGCATTCCATTAGGGGTGTAGAGGAAGAATCCTTTGCTGAAATAATTTCTGCACTTGGTAAAGATGTTGAGGCTCTAAATTCAACATCTATGATATTGACAGCAAAATCTCCTTATTCTTATGGAAAATTGATAGCAAGAGCAGCAAGGTTGGCAGTGATTGCTGAAGAAGTGAGATCTCTTGAAGTGGTTCCAGAAATAAGAAAGTTCCTAATGGGCGCAATTGAGCCTTGGTTAAATGGGACATTTGAGGGGAATGGTTTTGTATATGATGACAAATGGGGTGGAATTGTCAGCAAAGAAGGGTCCTTCGATTCTGGCGCAGACTTTGGATTTGGAATGTATAATAATCACCATCATCAGCTGGGCTACTTTCTTTATGCCATTGCTGTGCTTGTGAAAATTGACCCTGCTTGGGGGAGAAAGTACAGTCCTCAAGTTTATTCTCTTATGGCAGACATCATGAACTTGAGCAGGCGAGCCAATTCAAAGTTTCCAAAGTTGAGATGTTTTGATGTGTATAAATTGCATTCTTGGGGTACAGGATTGGCCGAATTCACGGATGGCCGGAGTCAAGAGAGCATCAGTGAAGCAGTAAATGCTTACTATTCTGCAGCTTTGGTGGGGTTAGCTTATGGAGACGCCCATCTTGTATCCATTGGCTCTATGCTAGCAGCATTGGAGATTAAAGCAGGTCAGATGTGGTGGCAAGTAAGAGAAGGAGAAACAACTTTGTACCAGGAAGAATTTGTTAAGGAAAACAGGGTGGTGGGAGTATTGTGGTCTAACAAAAGGGACAGTGGATTATGGTTTGCTCCCAGTGAATGGAAAGAGTGTAGGCTTGGCATTCAGGTGTTACCCATATTGCCAATCACAGAGATGTTGCTCTCAGATGTTGGGTTCGTAAGGGAGCTTGTGAACTGGGCGTTGCCTTCTCTGGGAAGAGAAGGTGTTGGGGAAGGATGGAAAGGCTTTGTCTATGCCCTGGAAAGCATTTATGACAAAGATGGTTCTTTGCAGAAGATTAGGAACTTGAAAGATTTTGATGATGGAAACTCACTTACCAACCTGTTATGGTGGGTTCACAGTAGAGGCAAAGAAGGAGAAGCAAATATCAGTAACAGCAAAGTGCAACTAGTTTAG

mRNA sequence

ATGTTCAAGAAGATTGAAAGAGAGCTCAGGCGGGTAATCAAAGGGAAACGTCGCTCCCCTCCACCGCGTCTTCCACCACCACCTCCTCCTCCTCCTTCACCTCCACCTCCACCTCCACCACAGCCCCCCGAGTCACCACCACCAGAACCTTCTCAAAAGCCATCAGAGCCTACTCCATTCCTATTTCCTTCAACTCAATCCACAGTTCTCCCGGATCCTTCACTATTCTTTTCCCCTGAACTCCTCTCAGCCCCTCTCCCTACAAACAGTTTCTTCCAGAACCTCACTCTCAATAATGGCGACCAACCTGAGTACATCCATCCCTACTTGATCAAATCCTCCCTCTCCTCCATCTCTGTTTCCTACCCATCAATCTCCTCCAACTCTACTTCGGAATGCCAAATTTTCACCCCTGACCTTACCATCTCTTCCTCTGAAAAAATAAACCCACTTCCCCAGAAATCTCATGTAATCTCCTCATTCAATGATTTGAATGTCACTTTGGATATTCCTTCCTCCAACCTCCGTTTCTATCTCGTCCGGGGAAGCCCATTTCTGACTTTCACAGTTTCCAAAGGCGGAGCCTTTTCAATCTCCACCATTCACGAAGTTATCTCCTTTTCTTTTAACAATGCACTCACCAAATACACAATAAAATTGAAAAACAATCAAACATGGCTTATTTACTCCTCGTTTCCAATCAATTTGAGGCATAATCTTTCTATGATTACTTCTGGGGCATTTGCAGGAATCATTAGGATTGCAGCATTGCCAAATTTAGATCCGGAATGTGAACTGATACTTGACCGGTTTAGTTCGTGTTTCCCAGTCTCGGGTGAGGCACAGTTTACGAAGCCATTTTGTTTGGAATACAAATGGGAGACAAAAGGGTGGGGGGATTTGCTCATGCTTGCACACCCTCTGCATCTTCATCTCCTAGCAGGCACTGATAATGATGTAGTTATTTTAGATAAGCTCAAGTATAAAAGTATAGATGGTGAGCTTGTGGGTATGGTGGGAAGTTCATGGGCTTTGAAACCAGAACCTATTTCAGTAAGCTGGCATTCCATTAGGGGTGTAGAGGAAGAATCCTTTGCTGAAATAATTTCTGCACTTGGTAAAGATGTTGAGGCTCTAAATTCAACATCTATGATATTGACAGCAAAATCTCCTTATTCTTATGGAAAATTGATAGCAAGAGCAGCAAGGTTGGCAGTGATTGCTGAAGAAGTGAGATCTCTTGAAGTGGTTCCAGAAATAAGAAAGTTCCTAATGGGCGCAATTGAGCCTTGGTTAAATGGGACATTTGAGGGGAATGGTTTTGTATATGATGACAAATGGGGTGGAATTGTCAGCAAAGAAGGGTCCTTCGATTCTGGCGCAGACTTTGGATTTGGAATGTATAATAATCACCATCATCAGCTGGGCTACTTTCTTTATGCCATTGCTGTGCTTGTGAAAATTGACCCTGCTTGGGGGAGAAAGTACAGTCCTCAAGTTTATTCTCTTATGGCAGACATCATGAACTTGAGCAGGCGAGCCAATTCAAAGTTTCCAAAGTTGAGATGTTTTGATGTGTATAAATTGCATTCTTGGGGTACAGGATTGGCCGAATTCACGGATGGCCGGAGTCAAGAGAGCATCAGTGAAGCAGTAAATGCTTACTATTCTGCAGCTTTGGTGGGGTTAGCTTATGGAGACGCCCATCTTGTATCCATTGGCTCTATGCTAGCAGCATTGGAGATTAAAGCAGGTCAGATGTGGTGGCAAGTAAGAGAAGGAGAAACAACTTTGTACCAGGAAGAATTTGTTAAGGAAAACAGGGTGGTGGGAGTATTGTGGTCTAACAAAAGGGACAGTGGATTATGGTTTGCTCCCAGTGAATGGAAAGAGTGTAGGCTTGGCATTCAGGTGTTACCCATATTGCCAATCACAGAGATGTTGCTCTCAGATGTTGGGTTCGTAAGGGAGCTTGTGAACTGGGCGTTGCCTTCTCTGGGAAGAGAAGGTGTTGGGGAAGGATGGAAAGGCTTTGTCTATGCCCTGGAAAGCATTTATGACAAAGATGGTTCTTTGCAGAAGATTAGGAACTTGAAAGATTTTGATGATGGAAACTCACTTACCAACCTGTTATGGTGGGTTCACAGTAGAGGCAAAGAAGGAGAAGCAAATATCAGTAACAGCAAAGTGCAACTAGTTTAG

Coding sequence (CDS)

ATGTTCAAGAAGATTGAAAGAGAGCTCAGGCGGGTAATCAAAGGGAAACGTCGCTCCCCTCCACCGCGTCTTCCACCACCACCTCCTCCTCCTCCTTCACCTCCACCTCCACCTCCACCACAGCCCCCCGAGTCACCACCACCAGAACCTTCTCAAAAGCCATCAGAGCCTACTCCATTCCTATTTCCTTCAACTCAATCCACAGTTCTCCCGGATCCTTCACTATTCTTTTCCCCTGAACTCCTCTCAGCCCCTCTCCCTACAAACAGTTTCTTCCAGAACCTCACTCTCAATAATGGCGACCAACCTGAGTACATCCATCCCTACTTGATCAAATCCTCCCTCTCCTCCATCTCTGTTTCCTACCCATCAATCTCCTCCAACTCTACTTCGGAATGCCAAATTTTCACCCCTGACCTTACCATCTCTTCCTCTGAAAAAATAAACCCACTTCCCCAGAAATCTCATGTAATCTCCTCATTCAATGATTTGAATGTCACTTTGGATATTCCTTCCTCCAACCTCCGTTTCTATCTCGTCCGGGGAAGCCCATTTCTGACTTTCACAGTTTCCAAAGGCGGAGCCTTTTCAATCTCCACCATTCACGAAGTTATCTCCTTTTCTTTTAACAATGCACTCACCAAATACACAATAAAATTGAAAAACAATCAAACATGGCTTATTTACTCCTCGTTTCCAATCAATTTGAGGCATAATCTTTCTATGATTACTTCTGGGGCATTTGCAGGAATCATTAGGATTGCAGCATTGCCAAATTTAGATCCGGAATGTGAACTGATACTTGACCGGTTTAGTTCGTGTTTCCCAGTCTCGGGTGAGGCACAGTTTACGAAGCCATTTTGTTTGGAATACAAATGGGAGACAAAAGGGTGGGGGGATTTGCTCATGCTTGCACACCCTCTGCATCTTCATCTCCTAGCAGGCACTGATAATGATGTAGTTATTTTAGATAAGCTCAAGTATAAAAGTATAGATGGTGAGCTTGTGGGTATGGTGGGAAGTTCATGGGCTTTGAAACCAGAACCTATTTCAGTAAGCTGGCATTCCATTAGGGGTGTAGAGGAAGAATCCTTTGCTGAAATAATTTCTGCACTTGGTAAAGATGTTGAGGCTCTAAATTCAACATCTATGATATTGACAGCAAAATCTCCTTATTCTTATGGAAAATTGATAGCAAGAGCAGCAAGGTTGGCAGTGATTGCTGAAGAAGTGAGATCTCTTGAAGTGGTTCCAGAAATAAGAAAGTTCCTAATGGGCGCAATTGAGCCTTGGTTAAATGGGACATTTGAGGGGAATGGTTTTGTATATGATGACAAATGGGGTGGAATTGTCAGCAAAGAAGGGTCCTTCGATTCTGGCGCAGACTTTGGATTTGGAATGTATAATAATCACCATCATCAGCTGGGCTACTTTCTTTATGCCATTGCTGTGCTTGTGAAAATTGACCCTGCTTGGGGGAGAAAGTACAGTCCTCAAGTTTATTCTCTTATGGCAGACATCATGAACTTGAGCAGGCGAGCCAATTCAAAGTTTCCAAAGTTGAGATGTTTTGATGTGTATAAATTGCATTCTTGGGGTACAGGATTGGCCGAATTCACGGATGGCCGGAGTCAAGAGAGCATCAGTGAAGCAGTAAATGCTTACTATTCTGCAGCTTTGGTGGGGTTAGCTTATGGAGACGCCCATCTTGTATCCATTGGCTCTATGCTAGCAGCATTGGAGATTAAAGCAGGTCAGATGTGGTGGCAAGTAAGAGAAGGAGAAACAACTTTGTACCAGGAAGAATTTGTTAAGGAAAACAGGGTGGTGGGAGTATTGTGGTCTAACAAAAGGGACAGTGGATTATGGTTTGCTCCCAGTGAATGGAAAGAGTGTAGGCTTGGCATTCAGGTGTTACCCATATTGCCAATCACAGAGATGTTGCTCTCAGATGTTGGGTTCGTAAGGGAGCTTGTGAACTGGGCGTTGCCTTCTCTGGGAAGAGAAGGTGTTGGGGAAGGATGGAAAGGCTTTGTCTATGCCCTGGAAAGCATTTATGACAAAGATGGTTCTTTGCAGAAGATTAGGAACTTGAAAGATTTTGATGATGGAAACTCACTTACCAACCTGTTATGGTGGGTTCACAGTAGAGGCAAAGAAGGAGAAGCAAATATCAGTAACAGCAAAGTGCAACTAGTTTAG

Protein sequence

MFKKIERELRRVIKGKRRSPPPRLPPPPPPPPSPPPPPPPQPPESPPPEPSQKPSEPTPFLFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLSSISVSYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLRFYLVRGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLRHNLSMITSGAFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETKGWGDLLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVSWHSIRGVEEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLGYFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHSWGTGLAEFTDGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETTLYQEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFVRELVNWALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSRGKEGEANISNSKVQLV
Homology
BLAST of Lsi03G002190 vs. ExPASy Swiss-Prot
Match: Q9UT45 (Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 1.4e-35
Identity = 173/675 (25.63%), Postives = 294/675 (43.56%), Query Frame = 0

Query: 78  SPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLI----KSSLSSISVSYPS-----ISSN 137
           S + LS+P+ TN FF N  L +     Y  P+        ++  I +++       + ++
Sbjct: 74  STDSLSSPIETNKFFDNNLLGSRTNFMYADPFRYWWQSSDTMGGICIAHTDDNQRVMDTD 133

Query: 138 STSECQIFTPDLTISSSEKINPLPQKSH-VISSFNDLNV--TLDIPSSNLRFYLVRGSPF 197
            T     + P    S     + +   +  ++   + ++   T    SS+++  L  G   
Sbjct: 134 DTIPSYYYEPIGICSLGFGASGITSNTDPIVDEIDQMSARFTFSWDSSSMQLTLTEGMAV 193

Query: 198 LT--FTVSKGGAFSIST-IHEVISFSFNNALTKYTIKLKNNQTWLIY---SSFPINLRHN 257
            T  +T +    FS +  I++ +     +A+ KY + + +N  WLIY    S  +    +
Sbjct: 194 TTAVYTNAIPQIFSSTLYINDFVEVPGTSAVQKYRVTMSDNHVWLIYIYGDSLTLTESTS 253

Query: 258 LSMITSGAFAGIIRIAALPNLDPECELILDRFSSCF----PVSGEAQFTKPFCLEYKWET 317
             ++ S  F G I+IA +P  D   E + D ++  +     +SG  +    +   Y ++ 
Sbjct: 254 QMLVGSNTFNGYIQIAKIPLGDGTAEALYDTYAGVYITGISISGYVEDAVGY---YSFDF 313

Query: 318 KGWGD------LLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALK--- 377
              GD        +L H +    ++GT    ++L  L    + G++    G+S       
Sbjct: 314 TTAGDTSVEPLFFLLPHQVDT-AVSGTKVTSIVLASL----VSGDMNAAAGNSITFAEAI 373

Query: 378 PEPIS-VSWHSIR---GVEEESFAEIISALGKDV-EALNSTSMILTAKSPYSYGKLIARA 437
           P+ I  + W       G  EE+   I    G ++ E  ++ S +    S Y  GK++A+ 
Sbjct: 374 PQDIGFLPWSPTGGQIGYSEEALEIIAEVAGTELGEDFSANSNL---NSMYYSGKVLAKY 433

Query: 438 ARLAVIAEEVRSLEVVPE--IRKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEG-SFD 497
           A L V   ++   E   E  I+K L  A   +++   +     YD+ W G+VS  G S D
Sbjct: 434 AMLCVTINDILGDETSSEQCIQK-LEAAFARFVDNQ-QIYPLTYDNTWKGVVSVAGLSGD 493

Query: 498 SGADFGFGMYNNHHHQLGYFLYAIAVLVKIDPAWGRKYSPQ--VYSLMADIMNLSRRANS 557
           S ADFG   YN+HH   GYF++  AV+  IDP W    + +  V  L+ D+ N S   + 
Sbjct: 494 SLADFGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTGNNKEWVNFLVRDVANPSSN-DP 553

Query: 558 KFPKLRCFDVYKLHSWGTGLAEFTDGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSM 617
            FPK R  D+Y  H W +GL E  DG+ +ES SE  N ++   L G   GD+ +    ++
Sbjct: 554 YFPKHRMIDIYHGHGWASGLFESNDGKDEESTSEDYNFFFGMKLWGQVIGDSDMEDRANI 613

Query: 618 LAALEIKAGQMWWQVREGETTLYQEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGI 677
           +  +E  A   +    +G     Q   ++ N V G+ + NK     +F  +   E   GI
Sbjct: 614 ILGIERNALNKYMLYADGNV---QPTSMQPNYVAGITFMNKITHTTYFGTN--IEYIQGI 673

Query: 678 QVLPILPITEMLLSDVGFVRELVNW-ALPSLGREGVGEGWKGFVYALESIYDKDGSLQKI 707
            +LPI PI+  +     FV  L  W AL +   + V  GW+  +YA  +I + + S +  
Sbjct: 674 HMLPITPISAFIRGP-SFV--LAEWNALLASVIDYVDSGWRSLLYANLAIAEPEESYEYF 726

BLAST of Lsi03G002190 vs. ExPASy Swiss-Prot
Match: Q09850 (Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 2.6e-34
Identity = 165/677 (24.37%), Postives = 285/677 (42.10%), Query Frame = 0

Query: 63  PSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLSSISVSY 122
           P   S   P P L  +  L   P+ TN F+ NL +     P + HPY + + L+  S   
Sbjct: 14  PVFPSRAHPSPPLGLTSNLF--PIQTNKFYGNLYIGTRHNPSWSHPYSV-TWLNGSSYYG 73

Query: 123 PSISSNSTSECQIFTPD-LTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLRFYL-- 182
            +IS    S+ ++F PD  ++      NP    S +IS+    +  L     +  F +  
Sbjct: 74  LAISHIDDSQ-RVFGPDPESVPCQYYFNPAGLYSIIISAREFASGNLLSLDQSRHFSIQA 133

Query: 183 -----VRGSPFLTFTVSKGGAFSISTIHEVISFSFNNALT-------------KYTIKLK 242
                  GS  +   +  G  F +S  +  ++  FN+++              KY I+L 
Sbjct: 134 TLSATTSGSGTIILPIVAGMGF-VSGYYTNLTPVFNSSILFSSITKINFSRGYKYRIQLT 193

Query: 243 NNQTWLIY-------SSFPINLRHNLSMITSGAFAGIIRIAALPNLDPECELILDRFSSC 302
           + + W +Y       S+F + L  N  + TS  F G+I+I  +PN     E + + +   
Sbjct: 194 DGKIWFLYAFPTSSSSTFNLTLASNSQLTTSTKFTGLIQICKVPN-----ESVNNSYPDT 253

Query: 303 FPVSGEAQFTKPFCLE-----------YKWETKGWGDL--LMLAHPLHLHLLAGTDNDVV 362
              +    +T    L            +++ T G+ +L  LM A P H+    G+D    
Sbjct: 254 IYDASAGVYTTSISLSAQVSGTTGEYWFRFATAGYTNLNPLMFALPHHMQSF-GSDTQAY 313

Query: 363 ILDKLKYKSIDGELVGMVGSSWAL--KPEPISVSWHSI------RGVEEESFAEIISALG 422
                   +  G +      +W L  K  P  V +  I            + A I +A  
Sbjct: 314 KTGLGLASTTMGIMFAYATKTWHLIEKNLPTQVGFLPIPWNGGSNTYSPTALAAIRAACA 373

Query: 423 KDV--EALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLMGAIEPW 482
            D+  + +N++++     S Y+ GK++A  A++ ++A  +     +       +      
Sbjct: 374 TDINFDVVNASNL----DSMYTSGKIVAMYAQVCLVASRILGDSTLTNTGLTKLKQAMAR 433

Query: 483 LNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLGYFLYAIAVLVKIDPA 542
                +    VYD  + GI+S  G     AD+G   YN+HH   GY +YA AV+  +DP+
Sbjct: 434 FTTNTQMYPLVYDTTYKGIISTAGYSSPLADYGNTYYNDHHFHWGYHIYACAVIGLLDPS 493

Query: 543 W-GRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHSWGTGLAEFTDGRSQESISE 602
           W        V +L+ D  N S  +++ F   R FD +  HSW TG+ E  DG+ +ES SE
Sbjct: 494 WLVNDNIRYVNALLRDSANPS-ESDTYFAMFRNFDWFVGHSWATGIFESGDGKDEESTSE 553

Query: 603 AVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETTLYQEEFVKENRVV 662
             N  Y+  L G+   D  L++  +++  L +    +   +    TT  Q   +  N V 
Sbjct: 554 DFNFLYATKLWGMVRNDTVLINRANLM--LAVLKNSLNTYIYMTPTTSVQPSQILGNYVT 613

Query: 663 GVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFVRELVNWALPSLGREG 688
           G+ + NK D   +F+  E+  C+ GI ++P  PI+  L S   +V++  N  +  +    
Sbjct: 614 GITFMNKVDYATYFSAEEY--CKQGIHMIPTTPISGYLRSP-SYVQQDWNAKIAPI-INT 668

BLAST of Lsi03G002190 vs. ExPASy Swiss-Prot
Match: Q12168 (Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 3.2e-24
Identity = 191/785 (24.33%), Postives = 318/785 (40.51%), Query Frame = 0

Query: 24  LPPPPPPPPS-----PPPPPPPQPPESPPPEPSQKPSEP-----------TPFLFPSTQS 83
           +PPP P  P       PPP PP+    PP   S+  S+P           TP        
Sbjct: 8   IPPPVPNRPGGTTNRGPPPLPPRANVQPPVCSSENSSKPRENRVAGESLRTPSSSNPLAD 67

Query: 84  TVLPDPSLFFSPELLSA-------------------------PLPTNSFFQNLTLNNGDQ 143
           + +   ++F SP L +                          PL TN F+ N+ L++  Q
Sbjct: 68  SQVNSDNIFQSPVLSNLKAPPSVFNKVQHPVPKPNIDDQSVDPLETNKFYTNMLLDDNTQ 127

Query: 144 PEYIHPYLIKSSLSSISVSYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVIS--- 203
           P + HPY I  S          +++N T   Q      T       NP   KS V     
Sbjct: 128 PIWTHPYSIWFSRDP---ELFGLAANHTLASQRVFDTTTNPPRFYFNPTNIKSFVFKARE 187

Query: 204 --SFNDLNVTL-DIPSSNLRFYL-VRGSPFLTFTVSKGGAFSISTIHEV-------ISF- 263
             S ND+ +   D+   ++   + +  S F+ F + +G  F  +  H++       + F 
Sbjct: 188 FVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFPLVQGMGFVTAIYHDLGFELRSAVGFR 247

Query: 264 -----SFNNALTKYTIKLKNNQTWLIY---------SSFPINLRHNLSMITSGAFAGIIR 323
                S N    KY I+L+NN+ W++Y           F I+L  + ++I+S    G+I 
Sbjct: 248 SLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQDFQISLLDSNTIISSHKINGLI- 307

Query: 324 IAALPNLDPECELILDRFSSCFPV----SGEAQFTKPFCLEYKWETKGW---GDLLMLAH 383
                 L  +    +D  + C+PV    SG+          + +   G+   G  LM A 
Sbjct: 308 ----CQLSADSVPSIDMAAGCYPVYCDLSGQTVDEHFTNYRFNYTVAGYSQSGTTLMYAL 367

Query: 384 PLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKP--------EPISVSWHSIR 443
           P H         +  I   L   ++ G + G + +S+ ++         EP+++S +   
Sbjct: 368 PHHKAAFTPEMQEREIASSLD-STVKGLMTGYLTNSFDMQVQVPQELGFEPVALSLNKKA 427

Query: 444 GVEEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLE-VV 503
              +E  ++I  A  ++V+ L+         S Y  GK++A+ A +  +   +   E + 
Sbjct: 428 DYSQEKLSKIREAAVQEVQ-LSDPQQESNIDSMYFSGKILAKYAWILYVTHYILHDENLT 487

Query: 504 PEIRKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLGY 563
            E+   L  A+E +++   +     YD  W GI+S   S  S  DFG   YN+HH    Y
Sbjct: 488 KELLSKLTIAMERFISNQ-QVLPLNYDVSWKGIIS---SGSSSQDFGNSYYNDHHFHYSY 547

Query: 564 FLYAIAVLVKIDP--------AWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKL 623
            +   A++  +D         +W       V  L+ D   +    +  FP+ R FD +  
Sbjct: 548 HVITAAIISLVDSDLSGVTNNSWLENNRDWVECLIRDYSGVD-NDDPYFPQFRSFDWFNG 607

Query: 624 HSWGTGLAEFTDGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWW 683
           HSW  GL    DG+ +ES SE VN+ Y+  L GL  G++ L  I ++   +     Q ++
Sbjct: 608 HSWAKGLFPSGDGKDEESTSEDVNSCYAIKLWGLVTGNSKLTDIANLQLGIMRNVFQSYF 667

Query: 684 QVREGETTLYQEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPIT--EM 709
            + E   T+  +EF+  N+V G+L+ NK D   +F           I ++  +PIT    
Sbjct: 668 -LYESNNTVQPKEFI-GNKVSGILFENKIDHATYFGMEPQY-----IHMIHAIPITSASS 727

BLAST of Lsi03G002190 vs. ExPASy Swiss-Prot
Match: D4AZ24 (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 2.1e-23
Identity = 200/784 (25.51%), Postives = 321/784 (40.94%), Query Frame = 0

Query: 12  VIKG----KRRSPPPRLPPPPP------------PPPSPPPPPPPQPPESPPPEPSQKPS 71
           ++KG    ++RSP P+  PP P               S P P    P    P +PS  P+
Sbjct: 127 IVKGFDSIEKRSPAPQRHPPAPTKATAGYQFTNCTSTSNPGPTATSPTSGIPSQPSAPPA 186

Query: 72  -------------EPTPFLFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQ 131
                        +P P    S        P      E  + P+ TN F+ N  L N   
Sbjct: 187 TMAGQDIFQPIAKDPIPANIKSRDD----HPVKANHIENPTGPISTNKFYANFFLGNQTS 246

Query: 132 PEYIHPYLI-------KSSLSSISVSYPSISSNSTSECQIFTPDLTISSSEKINPLPQKS 191
             + HPY +        +S   +++S+   S  +T E     P   +     INP+  KS
Sbjct: 247 TTFTHPYTMIWAKGDKNASSFGMAISHVEPSQRATGEPNNKLPGNPV--RYYINPVGIKS 306

Query: 192 HVISSFN-DLNVTLDI-----------------PSSNLRFYLVRGSPFLTFTVSK-GGAF 251
            V+S+     + T+ +                  S ++ F LV+G  F+T   +    A 
Sbjct: 307 LVLSASELKESTTMSVAKPQAFSAQAILRPTGGSSESITFPLVQGMGFITAIYNNLQPAI 366

Query: 252 SISTIHEVI--SFSFNNALTKYTIKLKNNQTWLIY------SSFPINLRHNLSMITSGAF 311
             + +   +  + S    + KY I L++++ WL+Y      +   + L  N  +     F
Sbjct: 367 QSAVLFRKVEPAGSPQGGIFKYKITLEDDKNWLLYVIPENGADPKLKLEGNKLISGPTGF 426

Query: 312 AGIIRIAALPNLDPECELILDR----FSSCFPVSGEAQFTKPFCLEYKWETKGWG-DLLM 371
            G+I++A  P+ + E E I D+    +++   +SG          ++ +E  G G  L+M
Sbjct: 427 KGVIQVAKNPSAE-EGEGIYDKSAGSYATDIKISGSVGTDGTGTYKFSFEKAGKGAPLVM 486

Query: 372 LAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVS-----W---H 431
            A P H+        +     KL   +  G     VG SW +    + +S     W    
Sbjct: 487 YALPHHVESFDDATKNTKKNMKLS-TTTKGMATACVGDSWTMVEGNLPLSMDFAPWKPGS 546

Query: 432 SIRGVEEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIAR-AARLAVIAEEVRSL 491
           S +    E     I A+  + E      +     S Y  GK + + A  +  + E V   
Sbjct: 547 SSQVTLSEGAKNAIKAVAGN-ELSQDMELQTNLNSMYFSGKGLNKFAGAIYTVQELVGDK 606

Query: 492 EVVPEIRKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSF---DSGADFGFGMYNNH 551
                    L  + + +++   +    VYD+ W G+VS  G++   D+G DFG  +YN+H
Sbjct: 607 AAASGPLNSLKESFKRFVDNK-QQIPLVYDNVWKGVVS-SGTYEKGDTGLDFGNTLYNDH 666

Query: 552 HHQLGYFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHS 611
           H   GYF+   A+L K+DPAW       V  L+ D  N S   +  FP  R FD Y  HS
Sbjct: 667 HFHYGYFILTAAILGKLDPAWLDANKAYVNMLVRDSGN-SVDNDEHFPFSRAFDWYHGHS 726

Query: 612 WGTGLAEFTDGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQV 671
           W  GL E +DG+ QES SE     Y+  + G   GD  + + G+++  +  +    ++ +
Sbjct: 727 WAKGLFESSDGKDQESTSEDTMYAYAIKMWGKTSGDKSMEARGNLMLGILARTLNNYFLM 786

Query: 672 REGETTLYQEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSD 707
           +       Q +    N+V G+L+ NK D   +F  +   E   GI +LP+LP +    S 
Sbjct: 787 KNDNVN--QPKNFIGNKVTGILFENKIDHTTYFGAN--LEYIQGIHMLPLLPNSAYTRS- 846

BLAST of Lsi03G002190 vs. ExPASy TrEMBL
Match: A0A5A7T8V6 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001970 PE=3 SV=1)

HSP 1 Score: 1328.9 bits (3438), Expect = 0.0e+00
Identity = 676/733 (92.22%), Postives = 697/733 (95.09%), Query Frame = 0

Query: 1   MFKKIERELRRVIKGKRRSPPPRLPPPPPPPPSPPPPPPPQPPESPPPEPSQKPSEPTPF 60
           MFKKIERELRR  KGKR SPP RLPPPP     PPPPPPPQ PES PPEPSQKPSEPTPF
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPP-----PPPPPPPQTPESQPPEPSQKPSEPTPF 60

Query: 61  LFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLSSISV 120
           LFPSTQSTVLPDPS+FFSPELLSAPLP+NSFFQN TLNNGDQPEYIHPYLIKSSLSSISV
Sbjct: 61  LFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISV 120

Query: 121 SYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV 180
           SYPSI S   SECQIFTPDLTIS SEKINPLPQKSHVISSFNDL+VTLDIPSSNLRFYLV
Sbjct: 121 SYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLV 180

Query: 181 RGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLRHNL 240
           RGSPFLTFTVSKG AFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL HNL
Sbjct: 181 RGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNL 240

Query: 241 SMITSGAFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETKGWGD 300
           SMITSG FAGIIRIAALPN D ECE ILDRFSSC+PV GEAQFTKPFCLEYKWETKGWGD
Sbjct: 241 SMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGD 300

Query: 301 LLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVSWHSIRGV 360
           LLMLAHPLHL LL G+D++V+ILDK KYKSIDGELVG+VGSSWALKPEPISVSWHSIRGV
Sbjct: 301 LLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGV 360

Query: 361 EEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEVVPEI 420
           EEESFAEIISAL KDVEALNSTSMILT KSPYSYGKLIARAARLAVIAEEVRSLEVVPEI
Sbjct: 361 EEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEI 420

Query: 421 RKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLGYFLY 480
           RKFLMGAIEPWLNGTFEGNGF+YD+KWGGIV+KEG+FD  ADFGFG+YNNHHH LGYFLY
Sbjct: 421 RKFLMGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLY 480

Query: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHSWGTGLAEFT 540
           AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFD YKLHSWGTGLAEFT
Sbjct: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFT 540

Query: 541 DGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETTLYQ 600
           DGRSQES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGETTLY+
Sbjct: 541 DGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYK 600

Query: 601 EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFVRELVN 660
           EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITE+LLSDVGFVRELVN
Sbjct: 601 EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVN 660

Query: 661 WALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSRGKE 720
           WALPSLGREGVGEGWKGFV+ALESIYDKDGSLQKIRNLK+FDDGNSLTNLLWWVHSRGKE
Sbjct: 661 WALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKE 720

Query: 721 GEANISNSKVQLV 734
            E NISNSK+ LV
Sbjct: 721 -EENISNSKIHLV 727

BLAST of Lsi03G002190 vs. ExPASy TrEMBL
Match: E5GCT6 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1327.4 bits (3434), Expect = 0.0e+00
Identity = 675/733 (92.09%), Postives = 697/733 (95.09%), Query Frame = 0

Query: 1   MFKKIERELRRVIKGKRRSPPPRLPPPPPPPPSPPPPPPPQPPESPPPEPSQKPSEPTPF 60
           MFKKIERELRR  KGKR SPP RLPPPP     PPPPPPPQ PES PPEPSQKPSEPTPF
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPP-----PPPPPPPQTPESQPPEPSQKPSEPTPF 60

Query: 61  LFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLSSISV 120
           LFPSTQSTVLPDPS+FFSPELLSAPLP+NSFFQN TLNNGDQPEYIHPYLIKSSLSSISV
Sbjct: 61  LFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISV 120

Query: 121 SYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV 180
           SYPSI S   SECQIFTPDLTIS SEKINPLPQKSHVISSFNDL+VTLDIPSSNLRFYLV
Sbjct: 121 SYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLV 180

Query: 181 RGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLRHNL 240
           RGSPFLTFTVSKG AFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL HNL
Sbjct: 181 RGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNL 240

Query: 241 SMITSGAFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETKGWGD 300
           SMITSG FAGIIRIAALPN D ECE ILDRFSSC+PV GEAQFTKPFCLEYKWETKGWGD
Sbjct: 241 SMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGD 300

Query: 301 LLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVSWHSIRGV 360
           LLMLAHPLHL LL G+D++V+ILDK KYKSIDGELVG+VGSSWALKPEPISVSWHSIRGV
Sbjct: 301 LLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGV 360

Query: 361 EEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEVVPEI 420
           EEESFAEIISAL KDVEALNSTSMILT KSPYSYGKLIARAARLAVIAEEVRSLEVVPEI
Sbjct: 361 EEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEI 420

Query: 421 RKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLGYFLY 480
           RKFL+GAIEPWLNGTFEGNGF+YD+KWGGIV+KEG+FD  ADFGFG+YNNHHH LGYFLY
Sbjct: 421 RKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLY 480

Query: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHSWGTGLAEFT 540
           AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFD YKLHSWGTGLAEFT
Sbjct: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFT 540

Query: 541 DGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETTLYQ 600
           DGRSQES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGETTLY+
Sbjct: 541 DGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYK 600

Query: 601 EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFVRELVN 660
           EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITE+LLSDVGFVRELVN
Sbjct: 601 EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVN 660

Query: 661 WALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSRGKE 720
           WALPSLGREGVGEGWKGFV+ALESIYDKDGSLQKIRNLK+FDDGNSLTNLLWWVHSRGKE
Sbjct: 661 WALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKE 720

Query: 721 GEANISNSKVQLV 734
            E NISNSK+ LV
Sbjct: 721 -EENISNSKIHLV 727

BLAST of Lsi03G002190 vs. ExPASy TrEMBL
Match: A0A1S3CBD8 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=LOC103498569 PE=3 SV=1)

HSP 1 Score: 1327.4 bits (3434), Expect = 0.0e+00
Identity = 675/733 (92.09%), Postives = 697/733 (95.09%), Query Frame = 0

Query: 1   MFKKIERELRRVIKGKRRSPPPRLPPPPPPPPSPPPPPPPQPPESPPPEPSQKPSEPTPF 60
           MFKKIERELRR  KGKR SPP RLPPPP     PPPPPPPQ PES PPEPSQKPSEPTPF
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPP-----PPPPPPPQTPESQPPEPSQKPSEPTPF 60

Query: 61  LFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLSSISV 120
           LFPSTQSTVLPDPS+FFSPELLSAPLP+NSFFQN TLNNGDQPEYIHPYLIKSSLSSISV
Sbjct: 61  LFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISV 120

Query: 121 SYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV 180
           SYPSI S   SECQIFTPDLTIS SEKINPLPQKSHVISSFNDL+VTLDIPSSNLRFYLV
Sbjct: 121 SYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLV 180

Query: 181 RGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLRHNL 240
           RGSPFLTFTVSKG AFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL HNL
Sbjct: 181 RGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNL 240

Query: 241 SMITSGAFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETKGWGD 300
           SMITSG FAGIIRIAALPN D ECE ILDRFSSC+PV GEAQFTKPFCLEYKWETKGWGD
Sbjct: 241 SMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGD 300

Query: 301 LLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVSWHSIRGV 360
           LLMLAHPLHL LL G+D++V+ILDK KYKSIDGELVG+VGSSWALKPEPISVSWHSIRGV
Sbjct: 301 LLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGV 360

Query: 361 EEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEVVPEI 420
           EEESFAEIISAL KDVEALNSTSMILT KSPYSYGKLIARAARLAVIAEEVRSLEVVPEI
Sbjct: 361 EEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEI 420

Query: 421 RKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLGYFLY 480
           RKFL+GAIEPWLNGTFEGNGF+YD+KWGGIV+KEG+FD  ADFGFG+YNNHHH LGYFLY
Sbjct: 421 RKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLY 480

Query: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHSWGTGLAEFT 540
           AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFD YKLHSWGTGLAEFT
Sbjct: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFT 540

Query: 541 DGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETTLYQ 600
           DGRSQES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGETTLY+
Sbjct: 541 DGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYK 600

Query: 601 EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFVRELVN 660
           EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITE+LLSDVGFVRELVN
Sbjct: 601 EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVN 660

Query: 661 WALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSRGKE 720
           WALPSLGREGVGEGWKGFV+ALESIYDKDGSLQKIRNLK+FDDGNSLTNLLWWVHSRGKE
Sbjct: 661 WALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKE 720

Query: 721 GEANISNSKVQLV 734
            E NISNSK+ LV
Sbjct: 721 -EENISNSKIHLV 727

BLAST of Lsi03G002190 vs. ExPASy TrEMBL
Match: A0A0A0KUJ9 (Endo-1,3(4)-beta-glucanase OS=Cucumis sativus OX=3659 GN=Csa_5G643380 PE=3 SV=1)

HSP 1 Score: 1327.0 bits (3433), Expect = 0.0e+00
Identity = 680/737 (92.27%), Postives = 702/737 (95.25%), Query Frame = 0

Query: 1   MFKKIERELRRVIKGKRRSPP---PRLPPPPPPPPSPPP-PPPPQPPESPPPEPSQKPSE 60
           M KKIER L RVIKGKR SPP   P  PPPPPPPPSPPP PPPPQPPES PPEPSQKPSE
Sbjct: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60

Query: 61  PTPFLFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLS 120
           PTPFLFPSTQSTVLPDPS+FFSPELLSAPLP+NSFFQN TLNNGDQPEYIHPYLIKSSLS
Sbjct: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120

Query: 121 SISVSYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLR 180
           SISVSYPSI SNS SECQIFTPDLTIS SEKINPLPQKSHVISSFNDL+VTLDIPSSNLR
Sbjct: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180

Query: 181 FYLVRGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240
           FYLVRGSPFLTFTVSKG AFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL
Sbjct: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240

Query: 241 RHNLSMITSGAFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETK 300
            HNLSMITSG FAGIIRIAALPN D ECE ILDRFSSC+PV GEAQ TKPFCLEYKWETK
Sbjct: 241 THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK 300

Query: 301 GWGDLLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVSWHS 360
           GWGDLLMLAHPLHL LL G+D++VVILDK KYKSIDGELVG+VGSSWALKPEPISVSWHS
Sbjct: 301 GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360

Query: 361 IRGVEEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEV 420
           IRGVEEESFAEIISAL KDVEALNSTSMILT KSPYSYGKLIARAARLAVIAEEVRSLE+
Sbjct: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM 420

Query: 421 VPEIRKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLG 480
           VPEIRKFL+GAIEPWLNGTFEGNGFVYD+KWGGIVSKEG+FD  ADFGFGMYNNHHH LG
Sbjct: 421 VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG 480

Query: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHSWGTGL 540
           YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFD YKLHSWGTGL
Sbjct: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL 540

Query: 541 AEFTDGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGET 600
           +EFTDGRSQES+SEAVNAYYSAALVGLAYGDAHLVSIGS+LAALEIKAGQMWWQ+REGET
Sbjct: 541 SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET 600

Query: 601 TLYQEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFVR 660
           TLY+EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITE+LLSDVGFVR
Sbjct: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660

Query: 661 ELVNWALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHS 720
           ELVNWALPSLGREGVGEGWKGFV+ALESIYDKDGSLQKIRNLK+FDDGNSLTNLLWWVHS
Sbjct: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720

Query: 721 RGKEGEANISNSKVQLV 734
           RGKE E NI+NSK+ LV
Sbjct: 721 RGKE-EQNINNSKIHLV 736

BLAST of Lsi03G002190 vs. ExPASy TrEMBL
Match: A0A6J1BXH2 (Endo-1,3(4)-beta-glucanase OS=Momordica charantia OX=3673 GN=LOC111006593 PE=3 SV=1)

HSP 1 Score: 1294.6 bits (3349), Expect = 0.0e+00
Identity = 661/736 (89.81%), Postives = 689/736 (93.61%), Query Frame = 0

Query: 1   MFKKIERELRRVIKGKRR-SPPPRLPPPPPPPPSPPP--PPPPQPPESPPPEPSQKPSEP 60
           MFKKIERELRR IKGKRR SPP RLPPPPPPPP PPP  PPP QPP+SPPPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60

Query: 61  TPFLFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFP TQSTVLPDPS FFS ELLSAPLPTNSFFQN TLNNGDQPEYIHPYLIKSSLSS
Sbjct: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSISSNS SE QIFTPDLTI  SEKI PLPQKSHVISSFNDLNVTLDIPSSNLRF
Sbjct: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180

Query: 181 YLVRGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLR 240
           +LVRGSPFLTFTVSKG AFSIS  HEV+SFSFNNALTKYT+KLKNNQTWLIYSS PINL 
Sbjct: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240

Query: 241 HNLSMITSGAFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETKG 300
           HNLS+ITSG FAGIIRIAALPN DPECE ILDRFSSC+PVSGEA FTKPFC+EYKWET+G
Sbjct: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300

Query: 301 WGDLLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVSWHSI 360
           WGDLLMLAHPLH+ LLAGT+NDVVILDK KYKSIDG+LVG+VGS W LKPEP+SV WHSI
Sbjct: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360

Query: 361 RGVEEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEVV 420
           RGVEEESFAEIISAL KDVEALNST+M  T  SPYSYGKLIARAARLAVIAEEVR LEV+
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTTM--TTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420

Query: 421 PEIRKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLGY 480
           PEIRKFLMGAIEPWLNGTF+GNGFVYD KWGGIVSKEGSFDS ADFGFG+YNNHHH LGY
Sbjct: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480

Query: 481 FLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHSWGTGLA 540
           FLYAIAVLVKIDPAWGRKYSPQ YSLMADIMNLSRR+NSKFP+LRCFDVYKLHSWGTGL 
Sbjct: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 540

Query: 541 EFTDGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETT 600
           EF DGR QESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGE T
Sbjct: 541 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGE-T 600

Query: 601 LYQEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFVRE 660
           LYQEEFVKENR+VGVLWSNKRDSGLWFAP EW+EC+LGIQVLP+LPITE+L SDVGFVRE
Sbjct: 601 LYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRE 660

Query: 661 LVNWALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSR 720
           LVNW LPSLGREGVGEGWKGFVYALESIYDKDG+L+KIRNLKDFDDGNSLTNLLWWVHSR
Sbjct: 661 LVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSR 720

Query: 721 GKEGEANISNSKVQLV 734
           GKEGEANIS++KVQLV
Sbjct: 721 GKEGEANISSNKVQLV 733

BLAST of Lsi03G002190 vs. NCBI nr
Match: XP_038889399.1 (endo-1,3(4)-beta-glucanase 2-like [Benincasa hispida])

HSP 1 Score: 1362.4 bits (3525), Expect = 0.0e+00
Identity = 694/733 (94.68%), Postives = 705/733 (96.18%), Query Frame = 0

Query: 1   MFKKIERELRRVIKGKRRSPPPRLPPPPPPPPSPPPPPPPQPPESPPPEPSQKPSEPTPF 60
           MFKKIER LR VIKGKRR PPP LPPPPP PP PPPPPPPQPPES PPEPSQKPSEPTPF
Sbjct: 1   MFKKIERGLRWVIKGKRRFPPPHLPPPPPLPPPPPPPPPPQPPESQPPEPSQKPSEPTPF 60

Query: 61  LFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLSSISV 120
           LFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQN TLNNGDQPEYIHPYLIKSSLSSISV
Sbjct: 61  LFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISV 120

Query: 121 SYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV 180
           SYPSISSNS SECQIFTPDLTIS SEK+NPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV
Sbjct: 121 SYPSISSNSASECQIFTPDLTISPSEKMNPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV 180

Query: 181 RGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLRHNL 240
           RGSPFLTFTVSKG AFSISTIHEV+SFSFNNALTKYTIKLKNNQTWLIYSSFPINL HNL
Sbjct: 181 RGSPFLTFTVSKGIAFSISTIHEVLSFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNL 240

Query: 241 SMITSGAFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETKGWGD 300
           S+ITSG FAGIIRIAAL N DPECELILDRFSSC+PVSGEAQFTKPFCLEYKWETKGWGD
Sbjct: 241 SIITSGGFAGIIRIAALSNSDPECELILDRFSSCYPVSGEAQFTKPFCLEYKWETKGWGD 300

Query: 301 LLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVSWHSIRGV 360
           LLMLAHPLHL LLAG+DNDVVILDK KYKSIDGELVG+VGSSWALKPE ISVSWHSIRGV
Sbjct: 301 LLMLAHPLHLRLLAGSDNDVVILDKFKYKSIDGELVGVVGSSWALKPESISVSWHSIRGV 360

Query: 361 EEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEVVPEI 420
           EEESFAEIISALGKDV ALNSTS ILT KSPYSYGKLIARAARLAVIAEEVR LEVVPEI
Sbjct: 361 EEESFAEIISALGKDVGALNSTSKILTTKSPYSYGKLIARAARLAVIAEEVRFLEVVPEI 420

Query: 421 RKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLGYFLY 480
           RKFL+GAIEPWLNGTFEGNGFV+D+KWGGIVSKEGSFDSGADFGFGMYNNHHH LGYFLY
Sbjct: 421 RKFLVGAIEPWLNGTFEGNGFVHDEKWGGIVSKEGSFDSGADFGFGMYNNHHHHLGYFLY 480

Query: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHSWGTGLAEFT 540
           AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSR+ANSKFPKLRCFD YKLHSWGTGLAEFT
Sbjct: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRQANSKFPKLRCFDAYKLHSWGTGLAEFT 540

Query: 541 DGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETTLYQ 600
           DGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETTLY 
Sbjct: 541 DGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETTLYH 600

Query: 601 EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFVRELVN 660
           EEFVKENRVVGVLWSNKRD GLWFAPSEWKECRLGIQVLPILPITE+LLSD GFVRELVN
Sbjct: 601 EEFVKENRVVGVLWSNKRDCGLWFAPSEWKECRLGIQVLPILPITELLLSDAGFVRELVN 660

Query: 661 WALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSRGKE 720
           WALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSRGKE
Sbjct: 661 WALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSRGKE 720

Query: 721 GEANISNSKVQLV 734
           GE NISNSKVQLV
Sbjct: 721 GEKNISNSKVQLV 733

BLAST of Lsi03G002190 vs. NCBI nr
Match: KAA0039393.1 (putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo var. makuwa] >TYK00581.1 putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1328.9 bits (3438), Expect = 0.0e+00
Identity = 676/733 (92.22%), Postives = 697/733 (95.09%), Query Frame = 0

Query: 1   MFKKIERELRRVIKGKRRSPPPRLPPPPPPPPSPPPPPPPQPPESPPPEPSQKPSEPTPF 60
           MFKKIERELRR  KGKR SPP RLPPPP     PPPPPPPQ PES PPEPSQKPSEPTPF
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPP-----PPPPPPPQTPESQPPEPSQKPSEPTPF 60

Query: 61  LFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLSSISV 120
           LFPSTQSTVLPDPS+FFSPELLSAPLP+NSFFQN TLNNGDQPEYIHPYLIKSSLSSISV
Sbjct: 61  LFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISV 120

Query: 121 SYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV 180
           SYPSI S   SECQIFTPDLTIS SEKINPLPQKSHVISSFNDL+VTLDIPSSNLRFYLV
Sbjct: 121 SYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLV 180

Query: 181 RGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLRHNL 240
           RGSPFLTFTVSKG AFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL HNL
Sbjct: 181 RGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNL 240

Query: 241 SMITSGAFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETKGWGD 300
           SMITSG FAGIIRIAALPN D ECE ILDRFSSC+PV GEAQFTKPFCLEYKWETKGWGD
Sbjct: 241 SMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGD 300

Query: 301 LLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVSWHSIRGV 360
           LLMLAHPLHL LL G+D++V+ILDK KYKSIDGELVG+VGSSWALKPEPISVSWHSIRGV
Sbjct: 301 LLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGV 360

Query: 361 EEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEVVPEI 420
           EEESFAEIISAL KDVEALNSTSMILT KSPYSYGKLIARAARLAVIAEEVRSLEVVPEI
Sbjct: 361 EEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEI 420

Query: 421 RKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLGYFLY 480
           RKFLMGAIEPWLNGTFEGNGF+YD+KWGGIV+KEG+FD  ADFGFG+YNNHHH LGYFLY
Sbjct: 421 RKFLMGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLY 480

Query: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHSWGTGLAEFT 540
           AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFD YKLHSWGTGLAEFT
Sbjct: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFT 540

Query: 541 DGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETTLYQ 600
           DGRSQES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGETTLY+
Sbjct: 541 DGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYK 600

Query: 601 EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFVRELVN 660
           EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITE+LLSDVGFVRELVN
Sbjct: 601 EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVN 660

Query: 661 WALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSRGKE 720
           WALPSLGREGVGEGWKGFV+ALESIYDKDGSLQKIRNLK+FDDGNSLTNLLWWVHSRGKE
Sbjct: 661 WALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKE 720

Query: 721 GEANISNSKVQLV 734
            E NISNSK+ LV
Sbjct: 721 -EENISNSKIHLV 727

BLAST of Lsi03G002190 vs. NCBI nr
Match: XP_008459428.1 (PREDICTED: putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo] >ADN34285.1 beta-glucan-binding protein [Cucumis melo subsp. melo])

HSP 1 Score: 1327.4 bits (3434), Expect = 0.0e+00
Identity = 675/733 (92.09%), Postives = 697/733 (95.09%), Query Frame = 0

Query: 1   MFKKIERELRRVIKGKRRSPPPRLPPPPPPPPSPPPPPPPQPPESPPPEPSQKPSEPTPF 60
           MFKKIERELRR  KGKR SPP RLPPPP     PPPPPPPQ PES PPEPSQKPSEPTPF
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPP-----PPPPPPPQTPESQPPEPSQKPSEPTPF 60

Query: 61  LFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLSSISV 120
           LFPSTQSTVLPDPS+FFSPELLSAPLP+NSFFQN TLNNGDQPEYIHPYLIKSSLSSISV
Sbjct: 61  LFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISV 120

Query: 121 SYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV 180
           SYPSI S   SECQIFTPDLTIS SEKINPLPQKSHVISSFNDL+VTLDIPSSNLRFYLV
Sbjct: 121 SYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLV 180

Query: 181 RGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLRHNL 240
           RGSPFLTFTVSKG AFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL HNL
Sbjct: 181 RGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNL 240

Query: 241 SMITSGAFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETKGWGD 300
           SMITSG FAGIIRIAALPN D ECE ILDRFSSC+PV GEAQFTKPFCLEYKWETKGWGD
Sbjct: 241 SMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGD 300

Query: 301 LLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVSWHSIRGV 360
           LLMLAHPLHL LL G+D++V+ILDK KYKSIDGELVG+VGSSWALKPEPISVSWHSIRGV
Sbjct: 301 LLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGV 360

Query: 361 EEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEVVPEI 420
           EEESFAEIISAL KDVEALNSTSMILT KSPYSYGKLIARAARLAVIAEEVRSLEVVPEI
Sbjct: 361 EEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEI 420

Query: 421 RKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLGYFLY 480
           RKFL+GAIEPWLNGTFEGNGF+YD+KWGGIV+KEG+FD  ADFGFG+YNNHHH LGYFLY
Sbjct: 421 RKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLY 480

Query: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHSWGTGLAEFT 540
           AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFD YKLHSWGTGLAEFT
Sbjct: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFT 540

Query: 541 DGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETTLYQ 600
           DGRSQES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGETTLY+
Sbjct: 541 DGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYK 600

Query: 601 EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFVRELVN 660
           EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITE+LLSDVGFVRELVN
Sbjct: 601 EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVN 660

Query: 661 WALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSRGKE 720
           WALPSLGREGVGEGWKGFV+ALESIYDKDGSLQKIRNLK+FDDGNSLTNLLWWVHSRGKE
Sbjct: 661 WALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKE 720

Query: 721 GEANISNSKVQLV 734
            E NISNSK+ LV
Sbjct: 721 -EENISNSKIHLV 727

BLAST of Lsi03G002190 vs. NCBI nr
Match: XP_011656028.1 (endo-1,3(4)-beta-glucanase 2 [Cucumis sativus] >KGN52559.1 hypothetical protein Csa_008697 [Cucumis sativus])

HSP 1 Score: 1327.0 bits (3433), Expect = 0.0e+00
Identity = 680/737 (92.27%), Postives = 702/737 (95.25%), Query Frame = 0

Query: 1   MFKKIERELRRVIKGKRRSPP---PRLPPPPPPPPSPPP-PPPPQPPESPPPEPSQKPSE 60
           M KKIER L RVIKGKR SPP   P  PPPPPPPPSPPP PPPPQPPES PPEPSQKPSE
Sbjct: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60

Query: 61  PTPFLFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLS 120
           PTPFLFPSTQSTVLPDPS+FFSPELLSAPLP+NSFFQN TLNNGDQPEYIHPYLIKSSLS
Sbjct: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120

Query: 121 SISVSYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLR 180
           SISVSYPSI SNS SECQIFTPDLTIS SEKINPLPQKSHVISSFNDL+VTLDIPSSNLR
Sbjct: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180

Query: 181 FYLVRGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240
           FYLVRGSPFLTFTVSKG AFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL
Sbjct: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240

Query: 241 RHNLSMITSGAFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETK 300
            HNLSMITSG FAGIIRIAALPN D ECE ILDRFSSC+PV GEAQ TKPFCLEYKWETK
Sbjct: 241 THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK 300

Query: 301 GWGDLLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVSWHS 360
           GWGDLLMLAHPLHL LL G+D++VVILDK KYKSIDGELVG+VGSSWALKPEPISVSWHS
Sbjct: 301 GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360

Query: 361 IRGVEEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEV 420
           IRGVEEESFAEIISAL KDVEALNSTSMILT KSPYSYGKLIARAARLAVIAEEVRSLE+
Sbjct: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM 420

Query: 421 VPEIRKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLG 480
           VPEIRKFL+GAIEPWLNGTFEGNGFVYD+KWGGIVSKEG+FD  ADFGFGMYNNHHH LG
Sbjct: 421 VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG 480

Query: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHSWGTGL 540
           YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFD YKLHSWGTGL
Sbjct: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL 540

Query: 541 AEFTDGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGET 600
           +EFTDGRSQES+SEAVNAYYSAALVGLAYGDAHLVSIGS+LAALEIKAGQMWWQ+REGET
Sbjct: 541 SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET 600

Query: 601 TLYQEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFVR 660
           TLY+EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITE+LLSDVGFVR
Sbjct: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660

Query: 661 ELVNWALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHS 720
           ELVNWALPSLGREGVGEGWKGFV+ALESIYDKDGSLQKIRNLK+FDDGNSLTNLLWWVHS
Sbjct: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720

Query: 721 RGKEGEANISNSKVQLV 734
           RGKE E NI+NSK+ LV
Sbjct: 721 RGKE-EQNINNSKIHLV 736

BLAST of Lsi03G002190 vs. NCBI nr
Match: XP_022134296.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Momordica charantia])

HSP 1 Score: 1294.6 bits (3349), Expect = 0.0e+00
Identity = 661/736 (89.81%), Postives = 689/736 (93.61%), Query Frame = 0

Query: 1   MFKKIERELRRVIKGKRR-SPPPRLPPPPPPPPSPPP--PPPPQPPESPPPEPSQKPSEP 60
           MFKKIERELRR IKGKRR SPP RLPPPPPPPP PPP  PPP QPP+SPPPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60

Query: 61  TPFLFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFP TQSTVLPDPS FFS ELLSAPLPTNSFFQN TLNNGDQPEYIHPYLIKSSLSS
Sbjct: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSISSNS SE QIFTPDLTI  SEKI PLPQKSHVISSFNDLNVTLDIPSSNLRF
Sbjct: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180

Query: 181 YLVRGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLR 240
           +LVRGSPFLTFTVSKG AFSIS  HEV+SFSFNNALTKYT+KLKNNQTWLIYSS PINL 
Sbjct: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240

Query: 241 HNLSMITSGAFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETKG 300
           HNLS+ITSG FAGIIRIAALPN DPECE ILDRFSSC+PVSGEA FTKPFC+EYKWET+G
Sbjct: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300

Query: 301 WGDLLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVSWHSI 360
           WGDLLMLAHPLH+ LLAGT+NDVVILDK KYKSIDG+LVG+VGS W LKPEP+SV WHSI
Sbjct: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360

Query: 361 RGVEEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEVV 420
           RGVEEESFAEIISAL KDVEALNST+M  T  SPYSYGKLIARAARLAVIAEEVR LEV+
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTTM--TTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420

Query: 421 PEIRKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLGY 480
           PEIRKFLMGAIEPWLNGTF+GNGFVYD KWGGIVSKEGSFDS ADFGFG+YNNHHH LGY
Sbjct: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480

Query: 481 FLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDVYKLHSWGTGLA 540
           FLYAIAVLVKIDPAWGRKYSPQ YSLMADIMNLSRR+NSKFP+LRCFDVYKLHSWGTGL 
Sbjct: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 540

Query: 541 EFTDGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETT 600
           EF DGR QESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGE T
Sbjct: 541 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGE-T 600

Query: 601 LYQEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFVRE 660
           LYQEEFVKENR+VGVLWSNKRDSGLWFAP EW+EC+LGIQVLP+LPITE+L SDVGFVRE
Sbjct: 601 LYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRE 660

Query: 661 LVNWALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSR 720
           LVNW LPSLGREGVGEGWKGFVYALESIYDKDG+L+KIRNLKDFDDGNSLTNLLWWVHSR
Sbjct: 661 LVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSR 720

Query: 721 GKEGEANISNSKVQLV 734
           GKEGEANIS++KVQLV
Sbjct: 721 GKEGEANISSNKVQLV 733

BLAST of Lsi03G002190 vs. TAIR 10
Match: AT5G15870.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 894.4 bits (2310), Expect = 6.0e-260
Identity = 455/727 (62.59%), Postives = 566/727 (77.85%), Query Frame = 0

Query: 1   MFKKIERELRRVIKGKRRSPPPRLPPPPPPPPSPPPPPPPQPPESPPPEPSQKPSEPTPF 60
           M KK+ R+++ +I    + P  R P PPPP P PP P PP     P  + S    + TPF
Sbjct: 1   MLKKVRRKVKVLITKPFKKPKNRPPSPPPPLPLPPSPSPP-----PSQQMSSSRQKNTPF 60

Query: 61  LFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLSSISV 120
           LFP + S+VLPDPS FFS +LLS+PLPTNSFFQN TLNNGDQ EY HPY+IK S SS+S+
Sbjct: 61  LFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQAEYFHPYIIKPSTSSLSI 120

Query: 121 SYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV 180
           SYPS+S NS    + F  D+TI+ S+  +P  +KSH+ISSF+DL VTLD PSSNLRF+LV
Sbjct: 121 SYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSDLGVTLDFPSSNLRFFLV 180

Query: 181 RGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL-RHN 240
           RGSPF+TF+V+   + +ISTIH V+S S N + TKYT+KL NNQTWLIY+S PINL +  
Sbjct: 181 RGSPFITFSVN--SSITISTIHAVLSLSGNTSSTKYTVKLNNNQTWLIYASSPINLTKDG 240

Query: 241 LSMITSG-AFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETKGW 300
           +S I  G  F+GIIRI  LPN +P  E ILD FS  +PVSG+A FTKPF LEYKWE +G+
Sbjct: 241 VSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDADFTKPFALEYKWEKRGY 300

Query: 301 GDLLMLAHPLHLHLLAGTDNDVVILDKLKYKSIDGELVGMVGSSWALKPEPISVSWHSIR 360
           GDLLMLAHPLHL LL+  D  + +LD  KY SIDG+LVG++G SW LKP+P+SV+WHSI+
Sbjct: 301 GDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGDSWVLKPDPVSVTWHSIK 360

Query: 361 GVEEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKLIARAARLAVIAEEVRSLEVVP 420
           GV+E+S  EIISAL KDV AL+S++ +    S Y Y KLIARAARLA+IAEEV  L+V+P
Sbjct: 361 GVQEDSHQEIISALIKDVNALDSSAEV--TNSSYFYAKLIARAARLALIAEEVCYLDVIP 420

Query: 421 EIRKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSFDSGADFGFGMYNNHHHQLGYF 480
           +IR +L   IEPWLNG+F  NGF+YD KWGG+++K GS DSGADFGFG+YN+HH+ LGYF
Sbjct: 421 KIRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGADFGFGIYNDHHYHLGYF 480

Query: 481 LYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSR---RANSKFPKLRCFDVYKLHSWGTG 540
           +YAIAVL KIDP WG++Y PQ Y+LMAD + L +   ++NS +P+LRCFD++KLHSW  G
Sbjct: 481 VYAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNYPRLRCFDLFKLHSWAGG 540

Query: 541 LAEFTDGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGE 600
           L EF DGR+QES SEAVNAYYSAAL+GLAYGD HLV+  SM+  LEI A +MWWQV+E +
Sbjct: 541 LTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVLTLEIHAAKMWWQVKE-D 600

Query: 601 TTLYQEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITEMLLSDVGFV 660
             +Y ++F  ENRVVGVLWS KRDSGLWFAP EWKECRLGIQ+LP+LP++E+L SDV FV
Sbjct: 601 DAIYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPLLPVSEVLFSDVTFV 660

Query: 661 RELVNWALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVH 720
           ++LVNW +P+L R+ VGEGWKGFVYALES+YDKDG+++KI+ L  FDDGNSL+NLLWWVH
Sbjct: 661 KQLVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLNGFDDGNSLSNLLWWVH 717

Query: 721 SRGKEGE 723
           SR  + +
Sbjct: 721 SRNNDDD 717

BLAST of Lsi03G002190 vs. TAIR 10
Match: AT1G18310.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 795.8 bits (2054), Expect = 2.9e-230
Identity = 404/684 (59.06%), Postives = 492/684 (71.93%), Query Frame = 0

Query: 60  FLFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNLTLNNGDQPEYIHPYLIKSSLSSIS 119
           FLFP T+S+VLPDPS FFSP+LLS PLPTNSFFQN TL NGDQ EY HPYLIKS  SS+ 
Sbjct: 8   FLFPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVDSSLC 67

Query: 120 VSYPSISSNSTSECQIFTPDLTISSSEKINPLPQKSHVISSFNDLNVTLDIPSSNLRFYL 179
           +SYPS+  NS    ++FT D+ IS S    P  +K+H+ISSF+DL VTLD PSSNLRF+L
Sbjct: 68  ISYPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNLRFFL 127

Query: 180 VRGSPFLTFTVSKGGAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL-RH 239
           VRGSP                          ++ TK++ KL NNQ WLIY+S PI+L +H
Sbjct: 128 VRGSP--------------------------SSSTKWSDKLTNNQRWLIYASSPIDLTKH 187

Query: 240 NLSMI-TSGAFAGIIRIAALPNLDPECELILDRFSSCFPVSGEAQFTKPFCLEYKWETKG 299
             S I   G F GI+RI  LP  +P  E  LDRFSSC+PVSG+A FTKPF L+Y WE +G
Sbjct: 188 GDSSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMWEKRG 247

Query: 300 WGDLLMLAHPLHLHLLAG--------------------TDNDVVILDKLKYKSIDGELVG 359
            GDLLMLAHPLHL LLA                     T++ V +LD  +YKSIDG+LVG
Sbjct: 248 SGDLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDGDLVG 307

Query: 360 MVGSSWALKPEPISVSWHSIRGVEEESFAEIISALGKDVEALNSTSMILTAKSPYSYGKL 419
           +VG SW LKP+ +SV+WHS++GV+++S+ EIISALGKDV  LNS++ +    S Y YGKL
Sbjct: 308 VVGDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLNSSAEV--TSSSYFYGKL 367

Query: 420 IARAARLAVIAEEVRSLEVVPEIRKFLMGAIEPWLNGTFEGNGFVYDDKWGGIVSKEGSF 479
           IARAAR A+IAEEV  L+V+P+I  +L   IEPWL+G+F+ NGF+YD KWGG+++K+GS 
Sbjct: 368 IARAARFALIAEEVCYLDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITKQGSK 427

Query: 480 DSGADFGFGMYNNHHHQLGYFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRR---- 539
           DS ADFGFG+YN+HH+ +GYFLYAIAVL K DP WG +Y  Q YSL+AD M   R+    
Sbjct: 428 DSQADFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRKDDNN 487

Query: 540 ANSKFPKLRCFDVYKLHSWGTGLAEFTDGRSQESISEAVNAYYSAALVGLAYGDAHLVSI 599
           +NS +P+LR FD++KLHSW  GL EF DGR+QES SEAVNAYYSAAL+GLAYGD HLV  
Sbjct: 488 SNSSYPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKHLVET 547

Query: 600 GSMLAALEIKAGQMWWQVREGETTLYQEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECR 659
            S +  LEI A +MWWQV++GE  LY ++F  ENRVVGVLWS KRDS LWF P EWKECR
Sbjct: 548 ASTIMTLEIHAAKMWWQVKKGE-ALYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWKECR 607

Query: 660 LGIQVLPILPITEMLLSDVGFVRELVNWALPSLGREGVGEGWKGFVYALESIYDKDGSLQ 718
           LGIQ+LPILP              LVNW LP+L R GVGEGWKGF+YALES+YDKDG+++
Sbjct: 608 LGIQLLPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLYDKDGAIK 648

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9UT451.4e-3525.63Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 9... [more]
Q098502.6e-3424.37Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 /... [more]
Q121683.2e-2424.33Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S... [more]
D4AZ242.1e-2325.51Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain A... [more]
Match NameE-valueIdentityDescription
A0A5A7T8V60.0e+0092.22Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
E5GCT60.0e+0092.09Endo-1,3(4)-beta-glucanase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3CBD80.0e+0092.09Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=LOC103498569 PE=3 SV=1[more]
A0A0A0KUJ90.0e+0092.27Endo-1,3(4)-beta-glucanase OS=Cucumis sativus OX=3659 GN=Csa_5G643380 PE=3 SV=1[more]
A0A6J1BXH20.0e+0089.81Endo-1,3(4)-beta-glucanase OS=Momordica charantia OX=3673 GN=LOC111006593 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_038889399.10.0e+0094.68endo-1,3(4)-beta-glucanase 2-like [Benincasa hispida][more]
KAA0039393.10.0e+0092.22putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo var. makuwa] >TYK00581.1 put... [more]
XP_008459428.10.0e+0092.09PREDICTED: putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo] >ADN34285.1 beta... [more]
XP_011656028.10.0e+0092.27endo-1,3(4)-beta-glucanase 2 [Cucumis sativus] >KGN52559.1 hypothetical protein ... [more]
XP_022134296.10.0e+0089.81probable endo-1,3(4)-beta-glucanase ARB_01444 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G15870.16.0e-26062.59glycosyl hydrolase family 81 protein [more]
AT1G18310.12.9e-23059.06glycosyl hydrolase family 81 protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040720Glycosyl hydrolase family 81, C-terminal domainPFAMPF17652Glyco_hydro81Ccoord: 361..713
e-value: 3.7E-97
score: 325.7
NoneNo IPR availableGENE3D2.70.98.30coord: 76..270
e-value: 8.1E-41
score: 142.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 19..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..65
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..15
NoneNo IPR availablePANTHERPTHR31983:SF19GLYCOSIDE HYDROLASE FAMILY 81 PROTEINcoord: 1..722
IPR040451Glycosyl hydrolase family 81, N-terminalPFAMPF03639Glyco_hydro_81coord: 83..354
e-value: 1.1E-59
score: 202.2
IPR005200Endo-1,3(4)-beta-glucanasePANTHERPTHR31983ENDO-1,3(4)-BETA-GLUCANASE 1coord: 1..722

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G002190.1Lsi03G002190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
molecular_function GO:0008422 beta-glucosidase activity
molecular_function GO:0052861 glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group
molecular_function GO:0052862 glucan endo-1,4-beta-glucanase activity, C-3 substituted reducing group