Lsi03G001600 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G001600
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionBAG family molecular chaperone regulator 8, chloroplastic
Locationchr03: 2203763 .. 2205644 (-)
RNA-Seq ExpressionLsi03G001600
SyntenyLsi03G001600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAAATCGAAGCCCCATTCTCAGCCTTCTTACACATCCATCGTGGTGGGTGGCTCCCTCTTCTCCTTCTTCACTTCAACGCCACCATTTTCCCCCAAAACCCCAAACCCTCGAACAATCCCCATCCCTATCTCACTGATCAAGCTTCAAGCTTCAAACTTCAAGCCTCTAATGGCCTCTCATCACCATCCTCGTCAACCCCATTTACACCGGACCCATCTTCCCTCCGCCTGTTGCTGCGTCGGCGATTTCACCACTCCCTGCTTTCAAACCCACCACTCCTCTCCTCACCTTCCGCCGTCTCCCTCCGACCCACTCCTTCAAGCTCTTGCCTCTCAAATCCTTCAATCCGCCCAACTTCACCACTACCCAAGTCTCCGTACCCAAAAATTTCAATCGCATTATCAATTTCAATCCCTCAATCCCCAAAATCAGAAGAAGCAAGAAATTCCTCAAGTCGTCCACCATTCTACTGTTTCCTCTCTTCTCTCCCGCATCGAAGCCCTCGAATCCTCTCTTCCTCGAGTTTTTGGTTCTCATGTTCCTTCCCAATCTCTCCGGCACGTTGCTGCTAGTACAATCCAGACCCATTTTCGAGCGTTCCTTGTCCGCAGATCCCGAGCACTCAGAGAGCTTAAAGATCTCGCCATCATCAAGTCTAGATTTGAGTCTCTCCAATCGTCGCTGTCCAATGGATTCTACTTTGATCGTAACGTCATTTCTCTCGAAATAATGGATTTTCTTCTCCATCTCGATTCCATTCAGGTAATTGAAGTTTGGTGGCTGCCTTTCGTTTTTCTCTACTTTTATTGTAATTGATTTTTTCTGTGATCTTCTTCTCAGGGTAGTGATCCAATGGTCAAAGACAGCAAGAAGCCCCTTACCAGAGATTTGGTTCAGTTCTTGGAACGAATTGATAACTTTTCTGTGAAGAGGAACGGACTTCCAGTTAAGGCAATGAAAAATTTGAGGGTAGGTAAAAATGTTGCTAAATCTAGGGTTTCCTCTAATCCTAAGCATAAAAGCTATGAGAATCAGAAAGAGACTATTGAGGAATTAAAGAGCAGAGTTGAAAAGATTTGTGAGCGTTCGAGAATTTTTGAGAATGATGGGAAAGATTTGAATCTTAAAAGGTTTCACCATGTTGATGATGATGAGGAGGAGGAGGAGGATGAGGAGTCCCCTAGGATCATTATCAAAGAGAACAATCAAACTCGAAATCAGAATCTTGCAAAAGGCCATCAAATTCTTCAACCTAGAGTGAAGAAAAGTGTTAGATTTGCTGAGAATGGAAACTTATCAAGAGTTTTAGGCAGCAACAGTCCACATGAAGATTATGGTGTTATGGATGAAAGGGATTCAAGTGATGAACTTGTGGAGGATACTTGCAATGAAGCAGTGGAGATTAAGGAGTTTTCTGAGGAAGCTGAAGATGATGAAGAAGCACATGGAGATGGTGAATCTCCAGAGGTCAGTGATGGAGAAAAAAACCATAGAAGGAAGATGACTAGTGGCCATGGAAGTTCTGCATTCACTGCTCCCTTACCTTTGAAGATGGAGAACAAAGCTGATTTGATGAAGAACAGAAAGTCTGAAAATTTTGGGATGAGTATGTAAACTTTAACCAATTGTTAGCTTCTTTTAGATTTGGAAATCTCTATTGGTTATGTCATCATCTGGTTTCATTGTTTATGAATGTGATTCTCTGTTTTGTTTTGATCTCAAATACAAGAGGCTCATTTGCTTTCTAGGAAGCTTCATTCTTTGAAGAAGATGTGATATTTGTAATTGATGCTTCTTTAGTTTGTAAGAAGACCCTCTTGGCCACTAACTTGCCTGTGTTTTTCTGTAAATGGAGCCTCAGTTTTCATTTGTTTCATCA

mRNA sequence

CCAAAATCGAAGCCCCATTCTCAGCCTTCTTACACATCCATCGTGGTGGGTGGCTCCCTCTTCTCCTTCTTCACTTCAACGCCACCATTTTCCCCCAAAACCCCAAACCCTCGAACAATCCCCATCCCTATCTCACTGATCAAGCTTCAAGCTTCAAACTTCAAGCCTCTAATGGCCTCTCATCACCATCCTCGTCAACCCCATTTACACCGGACCCATCTTCCCTCCGCCTGTTGCTGCGTCGGCGATTTCACCACTCCCTGCTTTCAAACCCACCACTCCTCTCCTCACCTTCCGCCGTCTCCCTCCGACCCACTCCTTCAAGCTCTTGCCTCTCAAATCCTTCAATCCGCCCAACTTCACCACTACCCAAGTCTCCGTACCCAAAAATTTCAATCGCATTATCAATTTCAATCCCTCAATCCCCAAAATCAGAAGAAGCAAGAAATTCCTCAAGTCGTCCACCATTCTACTGTTTCCTCTCTTCTCTCCCGCATCGAAGCCCTCGAATCCTCTCTTCCTCGAGTTTTTGGTTCTCATGTTCCTTCCCAATCTCTCCGGCACGTTGCTGCTAGTACAATCCAGACCCATTTTCGAGCGTTCCTTGTCCGCAGATCCCGAGCACTCAGAGAGCTTAAAGATCTCGCCATCATCAAGTCTAGATTTGAGTCTCTCCAATCGTCGCTGTCCAATGGATTCTACTTTGATCGTAACGTCATTTCTCTCGAAATAATGGATTTTCTTCTCCATCTCGATTCCATTCAGGGTAGTGATCCAATGGTCAAAGACAGCAAGAAGCCCCTTACCAGAGATTTGGTTCAGTTCTTGGAACGAATTGATAACTTTTCTGTGAAGAGGAACGGACTTCCAGTTAAGGCAATGAAAAATTTGAGGGTAGGTAAAAATGTTGCTAAATCTAGGGTTTCCTCTAATCCTAAGCATAAAAGCTATGAGAATCAGAAAGAGACTATTGAGGAATTAAAGAGCAGAGTTGAAAAGATTTGTGAGCGTTCGAGAATTTTTGAGAATGATGGGAAAGATTTGAATCTTAAAAGGTTTCACCATGTTGATGATGATGAGGAGGAGGAGGAGGATGAGGAGTCCCCTAGGATCATTATCAAAGAGAACAATCAAACTCGAAATCAGAATCTTGCAAAAGGCCATCAAATTCTTCAACCTAGAGTGAAGAAAAGTGTTAGATTTGCTGAGAATGGAAACTTATCAAGAGTTTTAGGCAGCAACAGTCCACATGAAGATTATGGTGTTATGGATGAAAGGGATTCAAGTGATGAACTTGTGGAGGATACTTGCAATGAAGCAGTGGAGATTAAGGAGTTTTCTGAGGAAGCTGAAGATGATGAAGAAGCACATGGAGATGGTGAATCTCCAGAGGTCAGTGATGGAGAAAAAAACCATAGAAGGAAGATGACTAGTGGCCATGGAAGTTCTGCATTCACTGCTCCCTTACCTTTGAAGATGGAGAACAAAGCTGATTTGATGAAGAACAGAAAGTCTGAAAATTTTGGGATGAGTATGTAAACTTTAACCAATTGTTAGCTTCTTTTAGATTTGGAAATCTCTATTGGTTATGTCATCATCTGGTTTCATTGTTTATGAATGTGATTCTCTGTTTTGTTTTGATCTCAAATACAAGAGGCTCATTTGCTTTCTAGGAAGCTTCATTCTTTGAAGAAGATGTGATATTTGTAATTGATGCTTCTTTAGTTTGTAAGAAGACCCTCTTGGCCACTAACTTGCCTGTGTTTTTCTGTAAATGGAGCCTCAGTTTTCATTTGTTTCATCA

Coding sequence (CDS)

CCAAAATCGAAGCCCCATTCTCAGCCTTCTTACACATCCATCGTGGTGGGTGGCTCCCTCTTCTCCTTCTTCACTTCAACGCCACCATTTTCCCCCAAAACCCCAAACCCTCGAACAATCCCCATCCCTATCTCACTGATCAAGCTTCAAGCTTCAAACTTCAAGCCTCTAATGGCCTCTCATCACCATCCTCGTCAACCCCATTTACACCGGACCCATCTTCCCTCCGCCTGTTGCTGCGTCGGCGATTTCACCACTCCCTGCTTTCAAACCCACCACTCCTCTCCTCACCTTCCGCCGTCTCCCTCCGACCCACTCCTTCAAGCTCTTGCCTCTCAAATCCTTCAATCCGCCCAACTTCACCACTACCCAAGTCTCCGTACCCAAAAATTTCAATCGCATTATCAATTTCAATCCCTCAATCCCCAAAATCAGAAGAAGCAAGAAATTCCTCAAGTCGTCCACCATTCTACTGTTTCCTCTCTTCTCTCCCGCATCGAAGCCCTCGAATCCTCTCTTCCTCGAGTTTTTGGTTCTCATGTTCCTTCCCAATCTCTCCGGCACGTTGCTGCTAGTACAATCCAGACCCATTTTCGAGCGTTCCTTGTCCGCAGATCCCGAGCACTCAGAGAGCTTAAAGATCTCGCCATCATCAAGTCTAGATTTGAGTCTCTCCAATCGTCGCTGTCCAATGGATTCTACTTTGATCGTAACGTCATTTCTCTCGAAATAATGGATTTTCTTCTCCATCTCGATTCCATTCAGGGTAGTGATCCAATGGTCAAAGACAGCAAGAAGCCCCTTACCAGAGATTTGGTTCAGTTCTTGGAACGAATTGATAACTTTTCTGTGAAGAGGAACGGACTTCCAGTTAAGGCAATGAAAAATTTGAGGGTAGGTAAAAATGTTGCTAAATCTAGGGTTTCCTCTAATCCTAAGCATAAAAGCTATGAGAATCAGAAAGAGACTATTGAGGAATTAAAGAGCAGAGTTGAAAAGATTTGTGAGCGTTCGAGAATTTTTGAGAATGATGGGAAAGATTTGAATCTTAAAAGGTTTCACCATGTTGATGATGATGAGGAGGAGGAGGAGGATGAGGAGTCCCCTAGGATCATTATCAAAGAGAACAATCAAACTCGAAATCAGAATCTTGCAAAAGGCCATCAAATTCTTCAACCTAGAGTGAAGAAAAGTGTTAGATTTGCTGAGAATGGAAACTTATCAAGAGTTTTAGGCAGCAACAGTCCACATGAAGATTATGGTGTTATGGATGAAAGGGATTCAAGTGATGAACTTGTGGAGGATACTTGCAATGAAGCAGTGGAGATTAAGGAGTTTTCTGAGGAAGCTGAAGATGATGAAGAAGCACATGGAGATGGTGAATCTCCAGAGGTCAGTGATGGAGAAAAAAACCATAGAAGGAAGATGACTAGTGGCCATGGAAGTTCTGCATTCACTGCTCCCTTACCTTTGAAGATGGAGAACAAAGCTGATTTGATGAAGAACAGAAAGTCTGAAAATTTTGGGATGAGTATGTAA

Protein sequence

PKSKPHSQPSYTSIVVGGSLFSFFTSTPPFSPKTPNPRTIPIPISLIKLQASNFKPLMASHHHPRQPHLHRTHLPSACCCVGDFTTPCFQTHHSSPHLPPSPSDPLLQALASQILQSAQLHHYPSLRTQKFQSHYQFQSLNPQNQKKQEIPQVVHHSTVSSLLSRIEALESSLPRVFGSHVPSQSLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSNGFYFDRNVISLEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERIDNFSVKRNGLPVKAMKNLRVGKNVAKSRVSSNPKHKSYENQKETIEELKSRVEKICERSRIFENDGKDLNLKRFHHVDDDEEEEEDEESPRIIIKENNQTRNQNLAKGHQILQPRVKKSVRFAENGNLSRVLGSNSPHEDYGVMDERDSSDELVEDTCNEAVEIKEFSEEAEDDEEAHGDGESPEVSDGEKNHRRKMTSGHGSSAFTAPLPLKMENKADLMKNRKSENFGMSM
Homology
BLAST of Lsi03G001600 vs. ExPASy Swiss-Prot
Match: Q9LIB3 (BAG family molecular chaperone regulator 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAG1 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 2.8e-27
Identity = 133/422 (31.52%), Postives = 204/422 (48.34%), Query Frame = 0

Query: 58  MASHHHPRQPHL---HRTHLPSACCCVGDFTTPCFQTH----HSSPHLPPSPSDPLLQAL 117
           MASHHH    H+   H+ H            TP F T     + S H  P P++  L  L
Sbjct: 1   MASHHHHNHNHVCSRHQNH---------HNNTPQFATSPNCCNKSNHPSPPPAEDNLLHL 60

Query: 118 ASQILQSAQLHHYPSLRTQKFQSHYQFQSLNPQNQKKQEIPQVVHHSTVSSLLSRIEALE 177
            +  LQ+ Q     S  T   Q+    +S N   ++ + +P+      +S LL +I+ LE
Sbjct: 61  VATYLQNHQQETQCSCET-SCQNFNVIRSQNRVLRQHKNVPREYDQVVLSCLLRKIDDLE 120

Query: 178 SSLPRVFGSHVPSQ-----SLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESL 237
           SSL + F S    +     +LR  AA  IQTHFR++LV RS + R+LK+LA+IK+ F SL
Sbjct: 121 SSLNK-FSSFYDKRRDRHSTLRDSAARVIQTHFRSYLVHRSISFRQLKELAMIKASFLSL 180

Query: 238 QSSLSNGFYFDRNVISLEIMDFLLHLDSIQGS-DPMVKDSKKPLTRDLVQFLERIDNFSV 297
           +SS+S    F   V+S +  D LL LDSIQG  DPM++ SK+ L+RDLV+F++ +D+  V
Sbjct: 181 KSSVSGKLIFPFKVVSRKATDLLLQLDSIQGRIDPMIRSSKRSLSRDLVRFVQYVDDCVV 240

Query: 298 KRNGLPVKAMKNLRV-GKNVAKSRVSSNPKHKSYENQKETIEELKSRVEK---ICERSR- 357
           KR G  VK+   +++ GK       SS  +  + +   ++ E   S ++K      +SR 
Sbjct: 241 KRYGFVVKSGSGIKLNGKKPQGFGTSSEDEDNNADMSDDSEEVPVSSIDKRKVASSKSRT 300

Query: 358 --IFENDGKDLNLKRFHHVDDD-----------------EEEEEDEESPRIIIKENN--- 417
             + E D     + +F  +D +                 E++  D +   +++  +N   
Sbjct: 301 GVVIEGDVVKPPVMKFVVLDKNRNVCQVYGNRHDLTSSAEDDSVDGDEETLVMSRDNGRK 360

Query: 418 ---QTRNQNLAKGHQILQPRVKKSVRFAENGNLSRVLGSNSPHEDYGVMDERDSSDELVE 437
              + RN    KG      RV K+V F ENGN+ +V G      D     E D S ++ E
Sbjct: 361 QSLKARNGVSVKGGGGKTTRVVKTVSFDENGNVCKVYGDT---HDLTSSAEDDDSVDVGE 408

BLAST of Lsi03G001600 vs. ExPASy Swiss-Prot
Match: Q9LVA0 (BAG family molecular chaperone regulator 7 OS=Arabidopsis thaliana OX=3702 GN=BAG7 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.3e-08
Identity = 37/99 (37.37%), Postives = 61/99 (61.62%), Query Frame = 0

Query: 190 AASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSNGFY--------FDRNVIS 249
           AA  IQ  F+A+L+RRS++LR L+DLAI K++ + L++S  N  Y         +R   S
Sbjct: 306 AAVMIQRAFKAYLIRRSKSLRALRDLAIAKTKLKELRASFHNFSYRRLIARDGEERQKFS 365

Query: 250 LEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERID 281
            +I+  LL +D+I+G D MV+ +K+ +  +L   L+ +D
Sbjct: 366 EKIIVLLLTVDAIEGVDVMVRGAKRSMVDELEAMLDVVD 404

BLAST of Lsi03G001600 vs. ExPASy TrEMBL
Match: A0A0A0KXK1 (BAG domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G638400 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 3.3e-188
Identity = 373/462 (80.74%), Postives = 393/462 (85.06%), Query Frame = 0

Query: 58  MASHHH---PRQPHLHRTHLPSACCC----VGDFTTPCFQTHHSSPHLPPSPSDPLLQAL 117
           MASHHH   P QPH H+THLP ACCC     GDFTT CFQTHH SPHLP SPSDPLLQAL
Sbjct: 1   MASHHHPHQPHQPHFHQTHLPFACCCGGGGGGDFTTTCFQTHHPSPHLPVSPSDPLLQAL 60

Query: 118 ASQILQSAQLHHYPSLRTQK-FQSHYQFQSLNPQNQKKQEIPQVVHHSTVSSLLSRIEAL 177
           ASQILQSAQ H YP+LR+QK FQSHYQF  L PQNQKKQEIPQV+HHSTVSSLLSRIEAL
Sbjct: 61  ASQILQSAQFHQYPTLRSQKSFQSHYQF--LGPQNQKKQEIPQVLHHSTVSSLLSRIEAL 120

Query: 178 ESSLPRVFGSHVPSQSLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSL 237
           ESSLPRVF SH+PSQSLRHVAASTIQTHFRAFLVRRSRA  ELKDLAIIKSR+ESLQSS 
Sbjct: 121 ESSLPRVFASHLPSQSLRHVAASTIQTHFRAFLVRRSRAFSELKDLAIIKSRYESLQSSF 180

Query: 238 SNGFYFDRNVISLEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERIDNFSV-KRNG 297
           SNG YFDRN ISLEIMDFLLHLDSIQGSDPMVKDSKK L RDLVQFLERIDNF+V KRNG
Sbjct: 181 SNGLYFDRNAISLEIMDFLLHLDSIQGSDPMVKDSKKNLIRDLVQFLERIDNFAVKKRNG 240

Query: 298 LPVKAMKNLRVGKNVAKSRVSSNPKHKSYENQKETIEELKSRVEKICERSRIFENDGKDL 357
           LP     NLR+G+NV+K RV SNPKHK YEN KETIE+LK+RVEKICERSRI EN+ K  
Sbjct: 241 LP-----NLRLGQNVSKFRVPSNPKHKCYENHKETIEKLKNRVEKICERSRILENNVK-- 300

Query: 358 NLKRFHHVDDDEEEEEDEESPRIIIKENNQTRNQNLAKGHQILQPRVKKSVRFAENGNLS 417
                      EEEEE EESPRI IKE NQTRN+NL K HQILQPRVKKSVRFAENG+LS
Sbjct: 301 -----------EEEEELEESPRITIKEKNQTRNRNLVKNHQILQPRVKKSVRFAENGDLS 360

Query: 418 RVLGSNSPHEDYGVMDERDSSDELVEDTCNEAVEIKEFSEEAEDDEEAHG----DGESPE 477
           RVL S +PHEDYGVMDERDS DELVEDTCNEA+EIKEFSEEAEDDEE HG    DGESPE
Sbjct: 361 RVLSSKNPHEDYGVMDERDSGDELVEDTCNEAMEIKEFSEEAEDDEEGHGDEEVDGESPE 420

Query: 478 VSDGEKNHRRKMTSG-HGSSAFTAPLPLKMENKADLMKNRKS 506
           VSDGE+NHRRKM +G +G SAFTAPLPLKMENKADLMKNRKS
Sbjct: 421 VSDGERNHRRKMMTGNNGVSAFTAPLPLKMENKADLMKNRKS 442

BLAST of Lsi03G001600 vs. ExPASy TrEMBL
Match: A0A1S3CA01 (BAG family molecular chaperone regulator 8, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498524 PE=4 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 1.4e-186
Identity = 368/456 (80.70%), Postives = 388/456 (85.09%), Query Frame = 0

Query: 58  MASHHHPRQPHLHRTHLPSACCC----VGDFTTPCFQTHHSSPHLPPSPSDPLLQALASQ 117
           MASHHHP QPH H+ HLP ACCC     GDFTT CFQTH  SPHLP S SDPL+QALASQ
Sbjct: 1   MASHHHPHQPHFHQNHLPFACCCGGGGGGDFTTTCFQTHRPSPHLPASSSDPLIQALASQ 60

Query: 118 ILQSAQLHHYPSLRTQKFQSHYQFQSLNPQNQKKQEIPQVVHHSTVSSLLSRIEALESSL 177
           ILQSAQ H YP+LR+QKFQSHYQF  L PQ+QKKQEIPQV+HHSTVSSLLSRIEALESSL
Sbjct: 61  ILQSAQFHQYPTLRSQKFQSHYQF--LGPQSQKKQEIPQVLHHSTVSSLLSRIEALESSL 120

Query: 178 PRVFGSHVPSQSLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSNGF 237
           PRVF SH+PSQSLRHVAASTIQTHFRAFLVRRSRA  ELKDLAIIKSR+ESLQSS SNG 
Sbjct: 121 PRVFASHLPSQSLRHVAASTIQTHFRAFLVRRSRAFSELKDLAIIKSRYESLQSSFSNGL 180

Query: 238 YFDRNVISLEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERIDNFSV-KRNGLPVK 297
           YFDRN ISLEIMDFLLHLDSIQGSDPMVKDSKK L RDLVQFLERIDNFSV KRNGLP  
Sbjct: 181 YFDRNAISLEIMDFLLHLDSIQGSDPMVKDSKKNLIRDLVQFLERIDNFSVKKRNGLP-- 240

Query: 298 AMKNLRVGKNVAKSRVSSNPKHKSYENQKETIEELKSRVEKICERSRIFENDGKDLNLKR 357
              NLR G+NV+K RV SNPKHK  ENQKETIE+LK+RVEKICERSRI EN+ K      
Sbjct: 241 ---NLRSGQNVSKFRVPSNPKHKCCENQKETIEKLKNRVEKICERSRILENNVK------ 300

Query: 358 FHHVDDDEEEEEDEESPRIIIKENNQTRNQNLAKGHQILQPRVKKSVRFAENGNLSRVLG 417
                  EEEEE EESPRI IKE NQTRN+NL K HQILQPRVKKSVRFAENG+LSRVLG
Sbjct: 301 -------EEEEELEESPRITIKEKNQTRNRNLVKNHQILQPRVKKSVRFAENGDLSRVLG 360

Query: 418 SNSPHEDYGVMDERDSSDELVEDTCNEAVEIKEFSEEAEDDEEAH--GDGESPEVSDGEK 477
           S +PHEDYGVMDERDSSDELVEDTCNEA+EIKE SEEAEDDEE H  GDGESPEVSDGE+
Sbjct: 361 SKNPHEDYGVMDERDSSDELVEDTCNEAMEIKESSEEAEDDEEGHGDGDGESPEVSDGER 420

Query: 478 NHRRKMTSG-HGSSAFTAPLPLKMENKADLMKNRKS 506
           N RRKM +G +G SAFTAPLPLKMENKADLM NRKS
Sbjct: 421 NRRRKMMTGNNGVSAFTAPLPLKMENKADLMNNRKS 436

BLAST of Lsi03G001600 vs. ExPASy TrEMBL
Match: A0A5A7T904 (BAG family molecular chaperone regulator 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002540 PE=4 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 3.1e-186
Identity = 367/456 (80.48%), Postives = 388/456 (85.09%), Query Frame = 0

Query: 58  MASHHHPRQPHLHRTHLPSACCC----VGDFTTPCFQTHHSSPHLPPSPSDPLLQALASQ 117
           MASHHHP QPH H+ HLP ACCC     GDFTT CFQTH  SPHLP S SDPL+QALASQ
Sbjct: 1   MASHHHPHQPHFHQNHLPFACCCGGGGGGDFTTTCFQTHRPSPHLPASSSDPLIQALASQ 60

Query: 118 ILQSAQLHHYPSLRTQKFQSHYQFQSLNPQNQKKQEIPQVVHHSTVSSLLSRIEALESSL 177
           ILQSAQ H YP+LR+QKFQSHYQF  L PQ+QKKQEIPQV+HHSTVSSLLSRIEALESSL
Sbjct: 61  ILQSAQFHQYPTLRSQKFQSHYQF--LGPQSQKKQEIPQVLHHSTVSSLLSRIEALESSL 120

Query: 178 PRVFGSHVPSQSLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSNGF 237
           PRVF SH+PSQSLRHVAASTIQTHFRAFLVRRSRA  ELKDLAIIKSR+ESLQSS SNG 
Sbjct: 121 PRVFASHLPSQSLRHVAASTIQTHFRAFLVRRSRAFSELKDLAIIKSRYESLQSSFSNGL 180

Query: 238 YFDRNVISLEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERIDNFSV-KRNGLPVK 297
           YFDRN ISLEIMDFLLHLDSIQ SDPMVKDSKK L RDLVQFLERIDNF+V KRNGLP  
Sbjct: 181 YFDRNAISLEIMDFLLHLDSIQSSDPMVKDSKKNLIRDLVQFLERIDNFAVKKRNGLP-- 240

Query: 298 AMKNLRVGKNVAKSRVSSNPKHKSYENQKETIEELKSRVEKICERSRIFENDGKDLNLKR 357
              NLR G+NV+K RV SNPKHK  ENQKETIE+LK+RVEKICERSRI EN+ K      
Sbjct: 241 ---NLRSGQNVSKFRVPSNPKHKCCENQKETIEKLKNRVEKICERSRILENNVK------ 300

Query: 358 FHHVDDDEEEEEDEESPRIIIKENNQTRNQNLAKGHQILQPRVKKSVRFAENGNLSRVLG 417
                  EEEEE EESPRI IKENNQTRN+NL K HQILQPRVKKSVRFAENG+LSRVLG
Sbjct: 301 -------EEEEELEESPRITIKENNQTRNRNLVKNHQILQPRVKKSVRFAENGDLSRVLG 360

Query: 418 SNSPHEDYGVMDERDSSDELVEDTCNEAVEIKEFSEEAEDDEEAH--GDGESPEVSDGEK 477
           S +PHEDYGVMDERDSSDELVEDTCNEA+EIKE SEEAEDDEE H  GDGESPEVSDGE+
Sbjct: 361 SKNPHEDYGVMDERDSSDELVEDTCNEAMEIKESSEEAEDDEEGHGDGDGESPEVSDGER 420

Query: 478 NHRRKMTSG-HGSSAFTAPLPLKMENKADLMKNRKS 506
           N RRKM +G +G SAFTAPLPLKMENKADLM NRKS
Sbjct: 421 NRRRKMMTGNNGVSAFTAPLPLKMENKADLMNNRKS 436

BLAST of Lsi03G001600 vs. ExPASy TrEMBL
Match: A0A6J1JUX2 (BAG family molecular chaperone regulator 8, chloroplastic-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111487725 PE=4 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 3.9e-173
Identity = 353/465 (75.91%), Postives = 392/465 (84.30%), Query Frame = 0

Query: 58  MASHHHPRQP--HLHRTHLPSACCC---VGDFTTPCFQTHHSSPHLPPSPSDPLLQALAS 117
           MAS H+P QP  HLHRTHLPSACCC    GDF  PCFQT H SP  PPSPSDPLLQALAS
Sbjct: 1   MASRHYPHQPHLHLHRTHLPSACCCGCEGGDFANPCFQTLHPSPP-PPSPSDPLLQALAS 60

Query: 118 QILQSAQ---LHHYPSLRTQKFQSHYQFQSLNPQNQKK---QEIPQVVHHSTVSSLLSRI 177
           QILQSAQ   L HYP+LRT+KFQSHYQFQ L  QN K+   QE+PQV+HHST+SSL+SRI
Sbjct: 61  QILQSAQFQHLSHYPTLRTKKFQSHYQFQPLGSQNPKRVREQELPQVIHHSTISSLVSRI 120

Query: 178 EALESSLPRVFGSHVPSQSLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQ 237
           EALESSL RV GSHVP QSLR VAASTIQT+FRAFLVRRSRALRELKDLAIIKSRFESLQ
Sbjct: 121 EALESSLHRVSGSHVPPQSLRQVAASTIQTYFRAFLVRRSRALRELKDLAIIKSRFESLQ 180

Query: 238 SSLSNGFYFDRNVISLEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERIDNFSVKR 297
           SSL N FYFDRN ISLEIMD LLHLDSIQG+DPMVK SKK L+RDLVQFL+RIDN SVKR
Sbjct: 181 SSLCNEFYFDRNAISLEIMDLLLHLDSIQGNDPMVKGSKKSLSRDLVQFLDRIDNLSVKR 240

Query: 298 N-GLPVKAMKNLRVGKNVAKSRVSSNPKHKSYENQKETIEELKSRVEKICERSRIFENDG 357
           N GLP+K MKN  +G+NV+KSRVS+  + K+ ENQK TIE+LKSR+EKICERS+ FENDG
Sbjct: 241 NGGLPLKTMKNTGLGQNVSKSRVSTPKQLKNSENQKGTIEKLKSRIEKICERSKFFENDG 300

Query: 358 KDLNLKRFHHVDDDEEEEEDEESPRIIIKENNQTRNQNLAKGHQILQPRVKKSVRFAENG 417
           +DL+LKRFHHV DD++EEE++ESPRI  K  +QTR+QNL  GHQI QPRVKKSVRFAENG
Sbjct: 301 RDLDLKRFHHVGDDDDEEEEDESPRIHDKNRDQTRDQNLGNGHQI-QPRVKKSVRFAENG 360

Query: 418 NLSRVLGSNSPHEDYGVM---DERDSSDELVEDTCNEAVEIKEFSEEAEDD-EEAHGDGE 477
           +L RVLGSNSPHEDYGV+   DERDS DELVED  NEAVEIKEFSE AEDD EEAHG   
Sbjct: 361 SLFRVLGSNSPHEDYGVLDERDERDSGDELVEDIFNEAVEIKEFSEVAEDDEEEAHG--- 420

Query: 478 SPEVSDGEKNHRRKMTSGHGSSAFTAPLPLKMENKADL-MKNRKS 506
             E+SDGE+N  RKM+SG+G S+F+APLP+KMENKADL MKN KS
Sbjct: 421 --EMSDGERNPGRKMSSGNGGSSFSAPLPMKMENKADLIMKNSKS 458

BLAST of Lsi03G001600 vs. ExPASy TrEMBL
Match: A0A6J1JKG7 (BAG family molecular chaperone regulator 8, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487725 PE=4 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 5.1e-173
Identity = 352/467 (75.37%), Postives = 393/467 (84.15%), Query Frame = 0

Query: 58  MASHHHPRQP--HLHRTHLPSACCC---VGDFTTPCFQTHHSSPHLPPSPSDPLLQALAS 117
           MAS H+P QP  HLHRTHLPSACCC    GDF  PCFQT H SP  PPSPSDPLLQALAS
Sbjct: 1   MASRHYPHQPHLHLHRTHLPSACCCGCEGGDFANPCFQTLHPSPP-PPSPSDPLLQALAS 60

Query: 118 QILQSAQ---LHHYPSLRTQKFQSHYQFQSLNPQNQKK---QEIPQVVHHSTVSSLLSRI 177
           QILQSAQ   L HYP+LRT+KFQSHYQFQ L  QN K+   QE+PQV+HHST+SSL+SRI
Sbjct: 61  QILQSAQFQHLSHYPTLRTKKFQSHYQFQPLGSQNPKRVREQELPQVIHHSTISSLVSRI 120

Query: 178 EALESSLPRVFGSHVPSQSLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQ 237
           EALESSL RV GSHVP QSLR VAASTIQT+FRAFLVRRSRALRELKDLAIIKSRFESLQ
Sbjct: 121 EALESSLHRVSGSHVPPQSLRQVAASTIQTYFRAFLVRRSRALRELKDLAIIKSRFESLQ 180

Query: 238 SSLSNGFYFDRNVISLEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERIDNFSVKR 297
           SSL N FYFDRN ISLEIMD LLHLDSIQG+DPMVK SKK L+RDLVQFL+RIDN SVKR
Sbjct: 181 SSLCNEFYFDRNAISLEIMDLLLHLDSIQGNDPMVKGSKKSLSRDLVQFLDRIDNLSVKR 240

Query: 298 N-GLPVKAMKNLRVGKNVAKSRVSSNPKHKSYENQKETIEELKSRVEKICERSRIFENDG 357
           N GLP+K MKN  +G+NV+KSRVS+  + K+ ENQK TIE+LKSR+EKICERS+ FENDG
Sbjct: 241 NGGLPLKTMKNTGLGQNVSKSRVSTPKQLKNSENQKGTIEKLKSRIEKICERSKFFENDG 300

Query: 358 KDLNLKRFHHVDDDEEEEEDEESPRIIIKENNQTRNQNLAKGHQILQPRVKKSVRFAENG 417
           +DL+LKRFHHV DD++EEE++ESPRI  K  +QTR+QNL  GHQI QPRVKKSVRFAENG
Sbjct: 301 RDLDLKRFHHVGDDDDEEEEDESPRIHDKNRDQTRDQNLGNGHQI-QPRVKKSVRFAENG 360

Query: 418 NLSRVLGSNSPHEDYGVM---DERDSSDELVEDTCNEAVEIKEFSEEAEDD-EEAHGDGE 477
           +L RVLGSNSPHEDYGV+   DERDS DELVED  NEAVEIKEFSE AEDD EEAHG   
Sbjct: 361 SLFRVLGSNSPHEDYGVLDERDERDSGDELVEDIFNEAVEIKEFSEVAEDDEEEAHG--- 420

Query: 478 SPEVSDGEKNHRRKMTSGHGSSAFTAPLPLKMENKADL-MKNRKSEN 508
             E+SDGE+N  RKM+SG+G S+F+APLP+KMENKADL MKN  ++N
Sbjct: 421 --EMSDGERNPGRKMSSGNGGSSFSAPLPMKMENKADLIMKNNVNDN 460

BLAST of Lsi03G001600 vs. NCBI nr
Match: XP_038889083.1 (BAG family molecular chaperone regulator 8, chloroplastic [Benincasa hispida])

HSP 1 Score: 747.7 bits (1929), Expect = 6.8e-212
Identity = 399/460 (86.74%), Postives = 423/460 (91.96%), Query Frame = 0

Query: 58  MASHHHPRQPHLHRTHLPSACCC-VGDFTTPCFQTHHSSPHLPPSPSDPLLQALASQILQ 117
           MASHHHP QPH  RTHL SACCC  GDFTTPCFQTH     LPPSPSDPLLQALASQILQ
Sbjct: 1   MASHHHPHQPHFQRTHLTSACCCGGGDFTTPCFQTH----QLPPSPSDPLLQALASQILQ 60

Query: 118 SAQLHHYPSLRTQKFQSHYQFQSLNPQNQKKQEIPQVVHHSTVSSLLSRIEALESSLPRV 177
           SAQ HHYP+LR+QKFQSHYQF  LNPQNQKKQEIPQV+HHSTVSSLLSRIEALESSLPRV
Sbjct: 61  SAQFHHYPTLRSQKFQSHYQF--LNPQNQKKQEIPQVLHHSTVSSLLSRIEALESSLPRV 120

Query: 178 FGSHVPSQSLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSNGFYFD 237
           FGSH+PS SLRHVAASTIQTHFRAFLVRRSRA  ELKDLAIIKSRFESLQSSLSNGF+FD
Sbjct: 121 FGSHLPSPSLRHVAASTIQTHFRAFLVRRSRAFSELKDLAIIKSRFESLQSSLSNGFHFD 180

Query: 238 RNVISLEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERIDNFSVKRNGLPVKAMKN 297
           R  ISL+IMDFLLHLDSIQGSDPMVKDSKK L RDLVQFLERIDNF+VKRNGLPVKAMKN
Sbjct: 181 RKAISLKIMDFLLHLDSIQGSDPMVKDSKKSLIRDLVQFLERIDNFAVKRNGLPVKAMKN 240

Query: 298 LRVGKNVAKSRVSSNPKHKSYENQKETIEELKSRVEKICERSRIFENDGKDLNLKRFHHV 357
           LR+G++V+KSRVSSNPKHKSY+NQKETIEELKSRVEKICERSRIFE + KDLN KRFHHV
Sbjct: 241 LRLGQDVSKSRVSSNPKHKSYDNQKETIEELKSRVEKICERSRIFEKNEKDLNAKRFHHV 300

Query: 358 DDD---EEEEEDEESPRIIIKENNQTRNQNLAKGHQILQPRVKKSVRFAENGNLSRVLGS 417
           DDD   EEEEE+E+SPRIIIK+ NQTRN+NLA+ HQILQPRVKKSVRFAENGNLSRVLGS
Sbjct: 301 DDDDEEEEEEEEEQSPRIIIKDKNQTRNRNLAEAHQILQPRVKKSVRFAENGNLSRVLGS 360

Query: 418 NSPHEDYGVMDERDSSDELVEDTCNEAVEIKEFSEEAEDDEEAHGDGESPEVSDGEKNHR 477
           NSPHEDYGV+DERDSSDELVEDTCNEAVEIKEFSEEAED+EEAHGDG S EVSDGE+NHR
Sbjct: 361 NSPHEDYGVLDERDSSDELVEDTCNEAVEIKEFSEEAEDEEEAHGDGGSSEVSDGERNHR 420

Query: 478 RKMTSGHGSSAFTAPLPLKMENKADLMKNRKS-ENFGMSM 513
           RK++S HG S FT PLPLKMENKADLMKNRKS + FGMSM
Sbjct: 421 RKISSNHGGSTFTPPLPLKMENKADLMKNRKSLKFFGMSM 454

BLAST of Lsi03G001600 vs. NCBI nr
Match: XP_004141463.1 (BAG family molecular chaperone regulator 8, chloroplastic [Cucumis sativus] >KGN52501.1 hypothetical protein Csa_008310 [Cucumis sativus])

HSP 1 Score: 667.9 bits (1722), Expect = 6.9e-188
Identity = 373/462 (80.74%), Postives = 393/462 (85.06%), Query Frame = 0

Query: 58  MASHHH---PRQPHLHRTHLPSACCC----VGDFTTPCFQTHHSSPHLPPSPSDPLLQAL 117
           MASHHH   P QPH H+THLP ACCC     GDFTT CFQTHH SPHLP SPSDPLLQAL
Sbjct: 1   MASHHHPHQPHQPHFHQTHLPFACCCGGGGGGDFTTTCFQTHHPSPHLPVSPSDPLLQAL 60

Query: 118 ASQILQSAQLHHYPSLRTQK-FQSHYQFQSLNPQNQKKQEIPQVVHHSTVSSLLSRIEAL 177
           ASQILQSAQ H YP+LR+QK FQSHYQF  L PQNQKKQEIPQV+HHSTVSSLLSRIEAL
Sbjct: 61  ASQILQSAQFHQYPTLRSQKSFQSHYQF--LGPQNQKKQEIPQVLHHSTVSSLLSRIEAL 120

Query: 178 ESSLPRVFGSHVPSQSLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSL 237
           ESSLPRVF SH+PSQSLRHVAASTIQTHFRAFLVRRSRA  ELKDLAIIKSR+ESLQSS 
Sbjct: 121 ESSLPRVFASHLPSQSLRHVAASTIQTHFRAFLVRRSRAFSELKDLAIIKSRYESLQSSF 180

Query: 238 SNGFYFDRNVISLEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERIDNFSV-KRNG 297
           SNG YFDRN ISLEIMDFLLHLDSIQGSDPMVKDSKK L RDLVQFLERIDNF+V KRNG
Sbjct: 181 SNGLYFDRNAISLEIMDFLLHLDSIQGSDPMVKDSKKNLIRDLVQFLERIDNFAVKKRNG 240

Query: 298 LPVKAMKNLRVGKNVAKSRVSSNPKHKSYENQKETIEELKSRVEKICERSRIFENDGKDL 357
           LP     NLR+G+NV+K RV SNPKHK YEN KETIE+LK+RVEKICERSRI EN+ K  
Sbjct: 241 LP-----NLRLGQNVSKFRVPSNPKHKCYENHKETIEKLKNRVEKICERSRILENNVK-- 300

Query: 358 NLKRFHHVDDDEEEEEDEESPRIIIKENNQTRNQNLAKGHQILQPRVKKSVRFAENGNLS 417
                      EEEEE EESPRI IKE NQTRN+NL K HQILQPRVKKSVRFAENG+LS
Sbjct: 301 -----------EEEEELEESPRITIKEKNQTRNRNLVKNHQILQPRVKKSVRFAENGDLS 360

Query: 418 RVLGSNSPHEDYGVMDERDSSDELVEDTCNEAVEIKEFSEEAEDDEEAHG----DGESPE 477
           RVL S +PHEDYGVMDERDS DELVEDTCNEA+EIKEFSEEAEDDEE HG    DGESPE
Sbjct: 361 RVLSSKNPHEDYGVMDERDSGDELVEDTCNEAMEIKEFSEEAEDDEEGHGDEEVDGESPE 420

Query: 478 VSDGEKNHRRKMTSG-HGSSAFTAPLPLKMENKADLMKNRKS 506
           VSDGE+NHRRKM +G +G SAFTAPLPLKMENKADLMKNRKS
Sbjct: 421 VSDGERNHRRKMMTGNNGVSAFTAPLPLKMENKADLMKNRKS 442

BLAST of Lsi03G001600 vs. NCBI nr
Match: XP_008459375.1 (PREDICTED: BAG family molecular chaperone regulator 8, chloroplastic [Cucumis melo])

HSP 1 Score: 662.5 bits (1708), Expect = 2.9e-186
Identity = 368/456 (80.70%), Postives = 388/456 (85.09%), Query Frame = 0

Query: 58  MASHHHPRQPHLHRTHLPSACCC----VGDFTTPCFQTHHSSPHLPPSPSDPLLQALASQ 117
           MASHHHP QPH H+ HLP ACCC     GDFTT CFQTH  SPHLP S SDPL+QALASQ
Sbjct: 1   MASHHHPHQPHFHQNHLPFACCCGGGGGGDFTTTCFQTHRPSPHLPASSSDPLIQALASQ 60

Query: 118 ILQSAQLHHYPSLRTQKFQSHYQFQSLNPQNQKKQEIPQVVHHSTVSSLLSRIEALESSL 177
           ILQSAQ H YP+LR+QKFQSHYQF  L PQ+QKKQEIPQV+HHSTVSSLLSRIEALESSL
Sbjct: 61  ILQSAQFHQYPTLRSQKFQSHYQF--LGPQSQKKQEIPQVLHHSTVSSLLSRIEALESSL 120

Query: 178 PRVFGSHVPSQSLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSNGF 237
           PRVF SH+PSQSLRHVAASTIQTHFRAFLVRRSRA  ELKDLAIIKSR+ESLQSS SNG 
Sbjct: 121 PRVFASHLPSQSLRHVAASTIQTHFRAFLVRRSRAFSELKDLAIIKSRYESLQSSFSNGL 180

Query: 238 YFDRNVISLEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERIDNFSV-KRNGLPVK 297
           YFDRN ISLEIMDFLLHLDSIQGSDPMVKDSKK L RDLVQFLERIDNFSV KRNGLP  
Sbjct: 181 YFDRNAISLEIMDFLLHLDSIQGSDPMVKDSKKNLIRDLVQFLERIDNFSVKKRNGLP-- 240

Query: 298 AMKNLRVGKNVAKSRVSSNPKHKSYENQKETIEELKSRVEKICERSRIFENDGKDLNLKR 357
              NLR G+NV+K RV SNPKHK  ENQKETIE+LK+RVEKICERSRI EN+ K      
Sbjct: 241 ---NLRSGQNVSKFRVPSNPKHKCCENQKETIEKLKNRVEKICERSRILENNVK------ 300

Query: 358 FHHVDDDEEEEEDEESPRIIIKENNQTRNQNLAKGHQILQPRVKKSVRFAENGNLSRVLG 417
                  EEEEE EESPRI IKE NQTRN+NL K HQILQPRVKKSVRFAENG+LSRVLG
Sbjct: 301 -------EEEEELEESPRITIKEKNQTRNRNLVKNHQILQPRVKKSVRFAENGDLSRVLG 360

Query: 418 SNSPHEDYGVMDERDSSDELVEDTCNEAVEIKEFSEEAEDDEEAH--GDGESPEVSDGEK 477
           S +PHEDYGVMDERDSSDELVEDTCNEA+EIKE SEEAEDDEE H  GDGESPEVSDGE+
Sbjct: 361 SKNPHEDYGVMDERDSSDELVEDTCNEAMEIKESSEEAEDDEEGHGDGDGESPEVSDGER 420

Query: 478 NHRRKMTSG-HGSSAFTAPLPLKMENKADLMKNRKS 506
           N RRKM +G +G SAFTAPLPLKMENKADLM NRKS
Sbjct: 421 NRRRKMMTGNNGVSAFTAPLPLKMENKADLMNNRKS 436

BLAST of Lsi03G001600 vs. NCBI nr
Match: KAA0039443.1 (BAG family molecular chaperone regulator 8 [Cucumis melo var. makuwa] >TYK00632.1 BAG family molecular chaperone regulator 8 [Cucumis melo var. makuwa])

HSP 1 Score: 661.4 bits (1705), Expect = 6.4e-186
Identity = 367/456 (80.48%), Postives = 388/456 (85.09%), Query Frame = 0

Query: 58  MASHHHPRQPHLHRTHLPSACCC----VGDFTTPCFQTHHSSPHLPPSPSDPLLQALASQ 117
           MASHHHP QPH H+ HLP ACCC     GDFTT CFQTH  SPHLP S SDPL+QALASQ
Sbjct: 1   MASHHHPHQPHFHQNHLPFACCCGGGGGGDFTTTCFQTHRPSPHLPASSSDPLIQALASQ 60

Query: 118 ILQSAQLHHYPSLRTQKFQSHYQFQSLNPQNQKKQEIPQVVHHSTVSSLLSRIEALESSL 177
           ILQSAQ H YP+LR+QKFQSHYQF  L PQ+QKKQEIPQV+HHSTVSSLLSRIEALESSL
Sbjct: 61  ILQSAQFHQYPTLRSQKFQSHYQF--LGPQSQKKQEIPQVLHHSTVSSLLSRIEALESSL 120

Query: 178 PRVFGSHVPSQSLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSNGF 237
           PRVF SH+PSQSLRHVAASTIQTHFRAFLVRRSRA  ELKDLAIIKSR+ESLQSS SNG 
Sbjct: 121 PRVFASHLPSQSLRHVAASTIQTHFRAFLVRRSRAFSELKDLAIIKSRYESLQSSFSNGL 180

Query: 238 YFDRNVISLEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERIDNFSV-KRNGLPVK 297
           YFDRN ISLEIMDFLLHLDSIQ SDPMVKDSKK L RDLVQFLERIDNF+V KRNGLP  
Sbjct: 181 YFDRNAISLEIMDFLLHLDSIQSSDPMVKDSKKNLIRDLVQFLERIDNFAVKKRNGLP-- 240

Query: 298 AMKNLRVGKNVAKSRVSSNPKHKSYENQKETIEELKSRVEKICERSRIFENDGKDLNLKR 357
              NLR G+NV+K RV SNPKHK  ENQKETIE+LK+RVEKICERSRI EN+ K      
Sbjct: 241 ---NLRSGQNVSKFRVPSNPKHKCCENQKETIEKLKNRVEKICERSRILENNVK------ 300

Query: 358 FHHVDDDEEEEEDEESPRIIIKENNQTRNQNLAKGHQILQPRVKKSVRFAENGNLSRVLG 417
                  EEEEE EESPRI IKENNQTRN+NL K HQILQPRVKKSVRFAENG+LSRVLG
Sbjct: 301 -------EEEEELEESPRITIKENNQTRNRNLVKNHQILQPRVKKSVRFAENGDLSRVLG 360

Query: 418 SNSPHEDYGVMDERDSSDELVEDTCNEAVEIKEFSEEAEDDEEAH--GDGESPEVSDGEK 477
           S +PHEDYGVMDERDSSDELVEDTCNEA+EIKE SEEAEDDEE H  GDGESPEVSDGE+
Sbjct: 361 SKNPHEDYGVMDERDSSDELVEDTCNEAMEIKESSEEAEDDEEGHGDGDGESPEVSDGER 420

Query: 478 NHRRKMTSG-HGSSAFTAPLPLKMENKADLMKNRKS 506
           N RRKM +G +G SAFTAPLPLKMENKADLM NRKS
Sbjct: 421 NRRRKMMTGNNGVSAFTAPLPLKMENKADLMNNRKS 436

BLAST of Lsi03G001600 vs. NCBI nr
Match: XP_022991003.1 (BAG family molecular chaperone regulator 8, chloroplastic-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 617.8 bits (1592), Expect = 8.1e-173
Identity = 353/465 (75.91%), Postives = 392/465 (84.30%), Query Frame = 0

Query: 58  MASHHHPRQP--HLHRTHLPSACCC---VGDFTTPCFQTHHSSPHLPPSPSDPLLQALAS 117
           MAS H+P QP  HLHRTHLPSACCC    GDF  PCFQT H SP  PPSPSDPLLQALAS
Sbjct: 1   MASRHYPHQPHLHLHRTHLPSACCCGCEGGDFANPCFQTLHPSPP-PPSPSDPLLQALAS 60

Query: 118 QILQSAQ---LHHYPSLRTQKFQSHYQFQSLNPQNQKK---QEIPQVVHHSTVSSLLSRI 177
           QILQSAQ   L HYP+LRT+KFQSHYQFQ L  QN K+   QE+PQV+HHST+SSL+SRI
Sbjct: 61  QILQSAQFQHLSHYPTLRTKKFQSHYQFQPLGSQNPKRVREQELPQVIHHSTISSLVSRI 120

Query: 178 EALESSLPRVFGSHVPSQSLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQ 237
           EALESSL RV GSHVP QSLR VAASTIQT+FRAFLVRRSRALRELKDLAIIKSRFESLQ
Sbjct: 121 EALESSLHRVSGSHVPPQSLRQVAASTIQTYFRAFLVRRSRALRELKDLAIIKSRFESLQ 180

Query: 238 SSLSNGFYFDRNVISLEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERIDNFSVKR 297
           SSL N FYFDRN ISLEIMD LLHLDSIQG+DPMVK SKK L+RDLVQFL+RIDN SVKR
Sbjct: 181 SSLCNEFYFDRNAISLEIMDLLLHLDSIQGNDPMVKGSKKSLSRDLVQFLDRIDNLSVKR 240

Query: 298 N-GLPVKAMKNLRVGKNVAKSRVSSNPKHKSYENQKETIEELKSRVEKICERSRIFENDG 357
           N GLP+K MKN  +G+NV+KSRVS+  + K+ ENQK TIE+LKSR+EKICERS+ FENDG
Sbjct: 241 NGGLPLKTMKNTGLGQNVSKSRVSTPKQLKNSENQKGTIEKLKSRIEKICERSKFFENDG 300

Query: 358 KDLNLKRFHHVDDDEEEEEDEESPRIIIKENNQTRNQNLAKGHQILQPRVKKSVRFAENG 417
           +DL+LKRFHHV DD++EEE++ESPRI  K  +QTR+QNL  GHQI QPRVKKSVRFAENG
Sbjct: 301 RDLDLKRFHHVGDDDDEEEEDESPRIHDKNRDQTRDQNLGNGHQI-QPRVKKSVRFAENG 360

Query: 418 NLSRVLGSNSPHEDYGVM---DERDSSDELVEDTCNEAVEIKEFSEEAEDD-EEAHGDGE 477
           +L RVLGSNSPHEDYGV+   DERDS DELVED  NEAVEIKEFSE AEDD EEAHG   
Sbjct: 361 SLFRVLGSNSPHEDYGVLDERDERDSGDELVEDIFNEAVEIKEFSEVAEDDEEEAHG--- 420

Query: 478 SPEVSDGEKNHRRKMTSGHGSSAFTAPLPLKMENKADL-MKNRKS 506
             E+SDGE+N  RKM+SG+G S+F+APLP+KMENKADL MKN KS
Sbjct: 421 --EMSDGERNPGRKMSSGNGGSSFSAPLPMKMENKADLIMKNSKS 458

BLAST of Lsi03G001600 vs. TAIR 10
Match: AT3G29310.1 (calmodulin-binding protein-related )

HSP 1 Score: 124.8 bits (312), Expect = 2.0e-28
Identity = 133/422 (31.52%), Postives = 204/422 (48.34%), Query Frame = 0

Query: 58  MASHHHPRQPHL---HRTHLPSACCCVGDFTTPCFQTH----HSSPHLPPSPSDPLLQAL 117
           MASHHH    H+   H+ H            TP F T     + S H  P P++  L  L
Sbjct: 1   MASHHHHNHNHVCSRHQNH---------HNNTPQFATSPNCCNKSNHPSPPPAEDNLLHL 60

Query: 118 ASQILQSAQLHHYPSLRTQKFQSHYQFQSLNPQNQKKQEIPQVVHHSTVSSLLSRIEALE 177
            +  LQ+ Q     S  T   Q+    +S N   ++ + +P+      +S LL +I+ LE
Sbjct: 61  VATYLQNHQQETQCSCET-SCQNFNVIRSQNRVLRQHKNVPREYDQVVLSCLLRKIDDLE 120

Query: 178 SSLPRVFGSHVPSQ-----SLRHVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESL 237
           SSL + F S    +     +LR  AA  IQTHFR++LV RS + R+LK+LA+IK+ F SL
Sbjct: 121 SSLNK-FSSFYDKRRDRHSTLRDSAARVIQTHFRSYLVHRSISFRQLKELAMIKASFLSL 180

Query: 238 QSSLSNGFYFDRNVISLEIMDFLLHLDSIQGS-DPMVKDSKKPLTRDLVQFLERIDNFSV 297
           +SS+S    F   V+S +  D LL LDSIQG  DPM++ SK+ L+RDLV+F++ +D+  V
Sbjct: 181 KSSVSGKLIFPFKVVSRKATDLLLQLDSIQGRIDPMIRSSKRSLSRDLVRFVQYVDDCVV 240

Query: 298 KRNGLPVKAMKNLRV-GKNVAKSRVSSNPKHKSYENQKETIEELKSRVEK---ICERSR- 357
           KR G  VK+   +++ GK       SS  +  + +   ++ E   S ++K      +SR 
Sbjct: 241 KRYGFVVKSGSGIKLNGKKPQGFGTSSEDEDNNADMSDDSEEVPVSSIDKRKVASSKSRT 300

Query: 358 --IFENDGKDLNLKRFHHVDDD-----------------EEEEEDEESPRIIIKENN--- 417
             + E D     + +F  +D +                 E++  D +   +++  +N   
Sbjct: 301 GVVIEGDVVKPPVMKFVVLDKNRNVCQVYGNRHDLTSSAEDDSVDGDEETLVMSRDNGRK 360

Query: 418 ---QTRNQNLAKGHQILQPRVKKSVRFAENGNLSRVLGSNSPHEDYGVMDERDSSDELVE 437
              + RN    KG      RV K+V F ENGN+ +V G      D     E D S ++ E
Sbjct: 361 QSLKARNGVSVKGGGGKTTRVVKTVSFDENGNVCKVYGDT---HDLTSSAEDDDSVDVGE 408

BLAST of Lsi03G001600 vs. TAIR 10
Match: AT5G62390.1 (BCL-2-associated athanogene 7 )

HSP 1 Score: 62.8 bits (151), Expect = 9.4e-10
Identity = 37/99 (37.37%), Postives = 61/99 (61.62%), Query Frame = 0

Query: 190 AASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSNGFY--------FDRNVIS 249
           AA  IQ  F+A+L+RRS++LR L+DLAI K++ + L++S  N  Y         +R   S
Sbjct: 306 AAVMIQRAFKAYLIRRSKSLRALRDLAIAKTKLKELRASFHNFSYRRLIARDGEERQKFS 365

Query: 250 LEIMDFLLHLDSIQGSDPMVKDSKKPLTRDLVQFLERID 281
            +I+  LL +D+I+G D MV+ +K+ +  +L   L+ +D
Sbjct: 366 EKIIVLLLTVDAIEGVDVMVRGAKRSMVDELEAMLDVVD 404

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIB32.8e-2731.52BAG family molecular chaperone regulator 8, chloroplastic OS=Arabidopsis thalian... [more]
Q9LVA01.3e-0837.37BAG family molecular chaperone regulator 7 OS=Arabidopsis thaliana OX=3702 GN=BA... [more]
Match NameE-valueIdentityDescription
A0A0A0KXK13.3e-18880.74BAG domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G638400 PE=4 SV... [more]
A0A1S3CA011.4e-18680.70BAG family molecular chaperone regulator 8, chloroplastic OS=Cucumis melo OX=365... [more]
A0A5A7T9043.1e-18680.48BAG family molecular chaperone regulator 8 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A6J1JUX23.9e-17375.91BAG family molecular chaperone regulator 8, chloroplastic-like isoform X3 OS=Cuc... [more]
A0A6J1JKG75.1e-17375.37BAG family molecular chaperone regulator 8, chloroplastic-like isoform X2 OS=Cuc... [more]
Match NameE-valueIdentityDescription
XP_038889083.16.8e-21286.74BAG family molecular chaperone regulator 8, chloroplastic [Benincasa hispida][more]
XP_004141463.16.9e-18880.74BAG family molecular chaperone regulator 8, chloroplastic [Cucumis sativus] >KGN... [more]
XP_008459375.12.9e-18680.70PREDICTED: BAG family molecular chaperone regulator 8, chloroplastic [Cucumis me... [more]
KAA0039443.16.4e-18680.48BAG family molecular chaperone regulator 8 [Cucumis melo var. makuwa] >TYK00632.... [more]
XP_022991003.18.1e-17375.91BAG family molecular chaperone regulator 8, chloroplastic-like isoform X3 [Cucur... [more]
Match NameE-valueIdentityDescription
AT3G29310.12.0e-2831.52calmodulin-binding protein-related [more]
AT5G62390.19.4e-1037.37BCL-2-associated athanogene 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 314..344
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 407..431
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 444..512
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 461..476
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 445..460
NoneNo IPR availablePANTHERPTHR33322:SF18BAG FAMILY MOLECULAR CHAPERONE REGULATOR 8, CHLOROPLASTICcoord: 58..500
IPR040400BAG family molecular chaperone regulator 5/6/7/8PANTHERPTHR33322BAG DOMAIN CONTAINING PROTEIN, EXPRESSEDcoord: 58..500
IPR000048IQ motif, EF-hand binding sitePROSITEPS50096IQcoord: 187..216
score: 6.540401

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G001600.1Lsi03G001600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding
molecular_function GO:0051087 chaperone binding
molecular_function GO:0005515 protein binding