Lsi03G001440 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G001440
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Protein NUCLEAR FUSION DEFECTIVE 4-like
Location	chr03: 2064238 .. 2065950 (+)
RNA-Seq Expression	Lsi03G001440
Synteny	Lsi03G001440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTGGCGCAGGTTCAACTTACGTTTTTGGTACATACTCCAAAACAATGAAAACCCAATTCGATTACAACCAAACCCAAATCAACACATTGGGATTTGCCAAAGATCTCGGCTCTAACCTCGGTGTTTTCGCCGGCCTTCTCGGCGAGGTGGCGCCGCCGTGGGTTCTGTTCATCGTCGGTTCATCTCTCAATTTCTACAGCTACTTCATGATTTGGCTCTCCGTTACTCGTCGTATCGCCAAGCCCCAACTTTGGCAAATGTTCCTTTACATTTGCTTAGCTGCTAATTCACAAAATTTCGCTAATACAGCCGTTTTAGTAACCAGCGTCAGGAATTTCCCTGATCGACGAGGAATAATTCTTGGACTTCTTAAAGGGTTTGTTGGAATTGGAGGTGCCATTTTAACTCAATTCTATTTAGCCATTTATGGCCATGAAAATCCAGCTAGTTTGGTGCTTCTTCTTGCATGGTTCCCCACTTTTGTGTCTTTGGTATTTTCTCTCACGATTCGAACAATTAATATCCGTAAACATCCTGAAGAACTCAGAGTTCTTTACCATTTGCTTTATGTTTCAATAATTCTTGCTCTGTTTCTCTTATTTCTAACCATTACCCAAAAACAAGCTGTGTTTTCTTCAGTTGGGTATATAAGTGGAGCTGCCGTCATTGTTGGGTTACTATCTATTCCCCTTTTAATCGCCATTAGAGAAGAACTCGTGCTCTTCAAACTCAACAAACAAACTAATAATAATCCATCCGCCCCTGTTTTCATTCCTGAATTGAAAGCTTCAACAAATTCCCCATCTACAAATATTGATACTTCCTTAACCCCACTTGAAGAAATTTCAGAAACTAATTCACCGTCATGTTTCTCAAACATATTCGAGAAACCAGAAAGAGGAGAAGATTACACTATTTTACAAGCTCTGTTCAGCAAAGACATGGCACTTGTTTTCATGGGAACACTTTGTGGGTGTGGTTCATCAATTGCCGCCATTGACAACATAGGCCAAATTGGTGAATCACTTGGCTACCCATCTCAATCCATAAGCATCTTCGTTTCATGGGTTTCTATATTCAATTTCTTCGGCAGAGTATGCTCTGGTTTCATCTCTGAAACTCTTATGACAAAATACAAATTACCTCGTCCTTTAATGTTTGCTTTCTCCCATCTTCTCACCTGCATTGGAATGCTTTTCATTGCCTTCCCATATTCTGGATCAGTCTACGTAGCTTCATTGATAATCGGGTTCGGATTCGGAGCTCAAGTTCCGATGGTTTTCGCCATAGTTTCAGAGCTTTTCGGGTTGAAACATTACGCAACAATCTTCAATTGTGCACAATTGGCAGTCCCAATTGGATCATATGTGCTGAATGTGGATGTAATTGGGAGATTATATGATTTTGAAGCAGTAAAAGATGGTGGAATTAAGAATGGGAAAGGATTAACTTGCAATGGAGCTCATTGTTTTAGTGGGTCGTTCTTGATTTTGGCTGTGGTGGTGTTGATTGGAGCTTTGGCTTCGCTTGTGTTGGCTTTTAGGACAAAAGATTTTTACAAAGGGGATGTTTATAAGAAATATAGAGAGGATATGTGGATTCCTCAATCGGATATGGAATTTTATTGTTTAGATAACAAGAAGAAAGTTGTTAACAATATGCCAAGGATTGTCATGCCGCCTAAATATAGTTTTCTCAATATAATATAG

mRNA sequence

ATGATTGGCGCAGGTTCAACTTACGTTTTTGGTACATACTCCAAAACAATGAAAACCCAATTCGATTACAACCAAACCCAAATCAACACATTGGGATTTGCCAAAGATCTCGGCTCTAACCTCGGTGTTTTCGCCGGCCTTCTCGGCGAGGTGGCGCCGCCGTGGGTTCTGTTCATCGTCGGTTCATCTCTCAATTTCTACAGCTACTTCATGATTTGGCTCTCCGTTACTCGTCGTATCGCCAAGCCCCAACTTTGGCAAATGTTCCTTTACATTTGCTTAGCTGCTAATTCACAAAATTTCGCTAATACAGCCGTTTTAGTAACCAGCGTCAGGAATTTCCCTGATCGACGAGGAATAATTCTTGGACTTCTTAAAGGGTTTGTTGGAATTGGAGGTGCCATTTTAACTCAATTCTATTTAGCCATTTATGGCCATGAAAATCCAGCTAGTTTGGTGCTTCTTCTTGCATGGTTCCCCACTTTTGTGTCTTTGGTATTTTCTCTCACGATTCGAACAATTAATATCCGTAAACATCCTGAAGAACTCAGAGTTCTTTACCATTTGCTTTATGTTTCAATAATTCTTGCTCTGTTTCTCTTATTTCTAACCATTACCCAAAAACAAGCTGTGTTTTCTTCAGTTGGGTATATAAGTGGAGCTGCCGTCATTGTTGGGTTACTATCTATTCCCCTTTTAATCGCCATTAGAGAAGAACTCGTGCTCTTCAAACTCAACAAACAAACTAATAATAATCCATCCGCCCCTGTTTTCATTCCTGAATTGAAAGCTTCAACAAATTCCCCATCTACAAATATTGATACTTCCTTAACCCCACTTGAAGAAATTTCAGAAACTAATTCACCGTCATGTTTCTCAAACATATTCGAGAAACCAGAAAGAGGAGAAGATTACACTATTTTACAAGCTCTGTTCAGCAAAGACATGGCACTTGTTTTCATGGGAACACTTTGTGGGTGTGGTTCATCAATTGCCGCCATTGACAACATAGGCCAAATTGGTGAATCACTTGGCTACCCATCTCAATCCATAAGCATCTTCGTTTCATGGGTTTCTATATTCAATTTCTTCGGCAGAGTATGCTCTGGTTTCATCTCTGAAACTCTTATGACAAAATACAAATTACCTCGTCCTTTAATGTTTGCTTTCTCCCATCTTCTCACCTGCATTGGAATGCTTTTCATTGCCTTCCCATATTCTGGATCAGTCTACGTAGCTTCATTGATAATCGGGTTCGGATTCGGAGCTCAAGTTCCGATGGTTTTCGCCATAGTTTCAGAGCTTTTCGGGTTGAAACATTACGCAACAATCTTCAATTGTGCACAATTGGCAGTCCCAATTGGATCATATGTGCTGAATGTGGATGTAATTGGGAGATTATATGATTTTGAAGCAGTAAAAGATGGTGGAATTAAGAATGGGAAAGGATTAACTTGCAATGGAGCTCATTGTTTTAGTGGGTCGTTCTTGATTTTGGCTGTGGTGGTGTTGATTGGAGCTTTGGCTTCGCTTGTGTTGGCTTTTAGGACAAAAGATTTTTACAAAGGGGATGTTTATAAGAAATATAGAGAGGATATGTGGATTCCTCAATCGGATATGGAATTTTATTGTTTAGATAACAAGAAGAAAGTTGTTAACAATATGCCAAGGATTGTCATGCCGCCTAAATATAGTTTTCTCAATATAATATAG

Coding sequence (CDS)

ATGATTGGCGCAGGTTCAACTTACGTTTTTGGTACATACTCCAAAACAATGAAAACCCAATTCGATTACAACCAAACCCAAATCAACACATTGGGATTTGCCAAAGATCTCGGCTCTAACCTCGGTGTTTTCGCCGGCCTTCTCGGCGAGGTGGCGCCGCCGTGGGTTCTGTTCATCGTCGGTTCATCTCTCAATTTCTACAGCTACTTCATGATTTGGCTCTCCGTTACTCGTCGTATCGCCAAGCCCCAACTTTGGCAAATGTTCCTTTACATTTGCTTAGCTGCTAATTCACAAAATTTCGCTAATACAGCCGTTTTAGTAACCAGCGTCAGGAATTTCCCTGATCGACGAGGAATAATTCTTGGACTTCTTAAAGGGTTTGTTGGAATTGGAGGTGCCATTTTAACTCAATTCTATTTAGCCATTTATGGCCATGAAAATCCAGCTAGTTTGGTGCTTCTTCTTGCATGGTTCCCCACTTTTGTGTCTTTGGTATTTTCTCTCACGATTCGAACAATTAATATCCGTAAACATCCTGAAGAACTCAGAGTTCTTTACCATTTGCTTTATGTTTCAATAATTCTTGCTCTGTTTCTCTTATTTCTAACCATTACCCAAAAACAAGCTGTGTTTTCTTCAGTTGGGTATATAAGTGGAGCTGCCGTCATTGTTGGGTTACTATCTATTCCCCTTTTAATCGCCATTAGAGAAGAACTCGTGCTCTTCAAACTCAACAAACAAACTAATAATAATCCATCCGCCCCTGTTTTCATTCCTGAATTGAAAGCTTCAACAAATTCCCCATCTACAAATATTGATACTTCCTTAACCCCACTTGAAGAAATTTCAGAAACTAATTCACCGTCATGTTTCTCAAACATATTCGAGAAACCAGAAAGAGGAGAAGATTACACTATTTTACAAGCTCTGTTCAGCAAAGACATGGCACTTGTTTTCATGGGAACACTTTGTGGGTGTGGTTCATCAATTGCCGCCATTGACAACATAGGCCAAATTGGTGAATCACTTGGCTACCCATCTCAATCCATAAGCATCTTCGTTTCATGGGTTTCTATATTCAATTTCTTCGGCAGAGTATGCTCTGGTTTCATCTCTGAAACTCTTATGACAAAATACAAATTACCTCGTCCTTTAATGTTTGCTTTCTCCCATCTTCTCACCTGCATTGGAATGCTTTTCATTGCCTTCCCATATTCTGGATCAGTCTACGTAGCTTCATTGATAATCGGGTTCGGATTCGGAGCTCAAGTTCCGATGGTTTTCGCCATAGTTTCAGAGCTTTTCGGGTTGAAACATTACGCAACAATCTTCAATTGTGCACAATTGGCAGTCCCAATTGGATCATATGTGCTGAATGTGGATGTAATTGGGAGATTATATGATTTTGAAGCAGTAAAAGATGGTGGAATTAAGAATGGGAAAGGATTAACTTGCAATGGAGCTCATTGTTTTAGTGGGTCGTTCTTGATTTTGGCTGTGGTGGTGTTGATTGGAGCTTTGGCTTCGCTTGTGTTGGCTTTTAGGACAAAAGATTTTTACAAAGGGGATGTTTATAAGAAATATAGAGAGGATATGTGGATTCCTCAATCGGATATGGAATTTTATTGTTTAGATAACAAGAAGAAAGTTGTTAACAATATGCCAAGGATTGTCATGCCGCCTAAATATAGTTTTCTCAATATAATATAG

Protein sequence

MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIVGSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHPEELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREELVLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKPERGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVSIFNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGFGFGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKDGGIKNGKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWIPQSDMEFYCLDNKKKVVNNMPRIVMPPKYSFLNII

Homology

[Close]

BLAST of Lsi03G001440 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 4.6e-26
Identity = 135/524 (25.76%), Postives = 232/524 (44.27%), Query Frame = 0


Query: 5   GSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIVGSSL 64
           G+ + F  YS  +K+    +Q ++N L  A DLG   G  +G+     P  V+    +++
Sbjct: 58  GTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIALGYFPLSVVLFAAAAM 117

Query: 65  NFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGIILGL 124
            F  Y + WL +T  I  P    +FL   LA  S  + NTA  +  +R+FP+ R + L L
Sbjct: 118 GFVGYGVQWLVITNIITLPYS-LVFLCCLLAGLSICWFNTACFILCIRHFPNNRALALSL 177

Query: 125 LKGFVGIGGAILTQFYLAIYGHENPASLVL--LLAWFPTFVSLVFSLTIRTINIRKHPEE 184
              F GI  A+ +  + AI    +   L+L  L+    +F +L   LT  +++     + 
Sbjct: 178 TVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYPVLTKPSLDTTPDYDS 237

Query: 185 LRVLYHLLYVSIILALFLLF-LTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREELV 244
            R   H+  +  +LA+   F L ++      + + +I    ++V  L  PLL+  R +  
Sbjct: 238 RRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVVLLVFPLCAPLLVYAR-DYF 297

Query: 245 LFKLNKQTNNNPSAPVF--IPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKP 304
           L  +N + N+  S  V   I ELK    S S+   T    +    E N+           
Sbjct: 298 LPVINARLNHESSGYVMLNIDELKNQKTSVSSK--TGYEHMGTAKEGNT----------V 357

Query: 305 ERGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVS 364
             G++++    +   +  L ++   CG    +   +N+GQI +SLG   Q+ +  V+  S
Sbjct: 358 RLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG---QNSTTLVTIYS 417

Query: 365 IFNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYS--GSVYVASLII 424
            F+FFGR+ S    + +  +++L R   FA + L T I    +A   S   ++  A+ +I
Sbjct: 418 SFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALI 477

Query: 425 GFGFGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKDGG 484
           G   G       +I S+LFG        N     +PIGS +    +   +Y+  A  D  
Sbjct: 478 GLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY-IAASIYEANASPDIT 537

Query: 485 IKNGKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFY 522
                 + C G  C+  +F+    + ++G ++SL L  RTK  Y
Sbjct: 538 PIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

[Close]

BLAST of Lsi03G001440 vs. ExPASy TrEMBL
Match: A0A5D3D1P8 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2273G00170 PE=4 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 8.0e-276
Identity = 484/571 (84.76%), Postives = 529/571 (92.64%), Query Frame = 0


Query: 1   MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIV 60
           MIGAGSTYVFGTYSK +KTQF+Y+QT+INTLGFAKDLGSNLGVFAG LGE+APPWVL +V
Sbjct: 28  MIGAGSTYVFGTYSKVIKTQFNYSQTEINTLGFAKDLGSNLGVFAGFLGEIAPPWVLLVV 87

Query: 61  GSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGI 120
           G+ LNFYSYFMIWLSVTRRIAKPQLWQMF YICLAANSQNFANTAVLVTSVRNFPDRRGI
Sbjct: 88  GAFLNFYSYFMIWLSVTRRIAKPQLWQMFFYICLAANSQNFANTAVLVTSVRNFPDRRGI 147

Query: 121 ILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHP 180
           ILGLLKGFVGIGGAILTQF+LA+YGHENP++LVLLL+WFPT +SL F L+IRTINIR+HP
Sbjct: 148 ILGLLKGFVGIGGAILTQFFLALYGHENPSNLVLLLSWFPTLISLAFFLSIRTINIRRHP 207

Query: 181 EELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREEL 240
           EELRVLYHLLYVSIILALFLLFLTITQKQA FSS GY SGAAVIVGLLSIPLLIA+REEL
Sbjct: 208 EELRVLYHLLYVSIILALFLLFLTITQKQAAFSSAGYTSGAAVIVGLLSIPLLIAVREEL 267

Query: 241 VLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKPE 300
           +LFKL  QT+NNPS PVFIPE+K+S+NS   N + SLTP+EEI ETNSP+C SN+F KPE
Sbjct: 268 MLFKLKGQTDNNPSPPVFIPEMKSSSNSTPKN-NESLTPIEEIPETNSPTCLSNVFNKPE 327

Query: 301 RGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVSI 360
           RGED+TILQALFSKDM L+ +GTL GCGSSIAAIDNIGQIGESLGY S+SISIFVSWVSI
Sbjct: 328 RGEDFTILQALFSKDMGLILVGTLFGCGSSIAAIDNIGQIGESLGYTSKSISIFVSWVSI 387

Query: 361 FNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGFG 420
           FNFFGRV SGFISETLMTKYKLPRPLMFA +H  TCIGMLF+AFPY GS+Y ASLIIGFG
Sbjct: 388 FNFFGRVGSGFISETLMTKYKLPRPLMFACAHFFTCIGMLFVAFPYPGSIYGASLIIGFG 447

Query: 421 FGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKDGGIKN 480
           FGAQVPM+FAI+SELFGLK+YATIFNCAQLAVPIGSY+LNVDVIG+ YD EA K G  ++
Sbjct: 448 FGAQVPMLFAILSELFGLKYYATIFNCAQLAVPIGSYILNVDVIGKFYDHEATKGGSTRD 507

Query: 481 GKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWIPQSDM 540
           GKGLTC G HCFSGSFL+L+VVVLIG +ASLVLAFRT+DFYKGDVYKKYREDMWIPQSDM
Sbjct: 508 GKGLTCKGTHCFSGSFLVLSVVVLIGGVASLVLAFRTRDFYKGDVYKKYREDMWIPQSDM 567

Query: 541 EFYCLDNKKKVV-NNMPRIVMPPKYSFLNII 571
           EFYCLDNKKKVV NN+PR+VMPPKYSFLNII
Sbjct: 568 EFYCLDNKKKVVNNNLPRVVMPPKYSFLNII 597

[Close]

BLAST of Lsi03G001440 vs. ExPASy TrEMBL
Match: A0A1S3CMR5 (uncharacterized protein LOC103502570 OS=Cucumis melo OX=3656 GN=LOC103502570 PE=4 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 8.0e-276
Identity = 484/571 (84.76%), Postives = 529/571 (92.64%), Query Frame = 0


Query: 1   MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIV 60
           MIGAGSTYVFGTYSK +KTQF+Y+QT+INTLGFAKDLGSNLGVFAG LGE+APPWVL +V
Sbjct: 28  MIGAGSTYVFGTYSKVIKTQFNYSQTEINTLGFAKDLGSNLGVFAGFLGEIAPPWVLLVV 87

Query: 61  GSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGI 120
           G+ LNFYSYFMIWLSVTRRIAKPQLWQMF YICLAANSQNFANTAVLVTSVRNFPDRRGI
Sbjct: 88  GAFLNFYSYFMIWLSVTRRIAKPQLWQMFFYICLAANSQNFANTAVLVTSVRNFPDRRGI 147

Query: 121 ILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHP 180
           ILGLLKGFVGIGGAILTQF+LA+YGHENP++LVLLL+WFPT +SL F L+IRTINIR+HP
Sbjct: 148 ILGLLKGFVGIGGAILTQFFLALYGHENPSNLVLLLSWFPTLISLAFFLSIRTINIRRHP 207

Query: 181 EELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREEL 240
           EELRVLYHLLYVSIILALFLLFLTITQKQA FSS GY SGAAVIVGLLSIPLLIA+REEL
Sbjct: 208 EELRVLYHLLYVSIILALFLLFLTITQKQAAFSSAGYTSGAAVIVGLLSIPLLIAVREEL 267

Query: 241 VLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKPE 300
           +LFKL  QT+NNPS PVFIPE+K+S+NS   N + SLTP+EEI ETNSP+C SN+F KPE
Sbjct: 268 MLFKLKGQTDNNPSPPVFIPEMKSSSNSTPKN-NESLTPIEEIPETNSPTCLSNVFNKPE 327

Query: 301 RGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVSI 360
           RGED+TILQALFSKDM L+ +GTL GCGSSIAAIDNIGQIGESLGY S+SISIFVSWVSI
Sbjct: 328 RGEDFTILQALFSKDMGLILVGTLFGCGSSIAAIDNIGQIGESLGYTSKSISIFVSWVSI 387

Query: 361 FNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGFG 420
           FNFFGRV SGFISETLMTKYKLPRPLMFA +H  TCIGMLF+AFPY GS+Y ASLIIGFG
Sbjct: 388 FNFFGRVGSGFISETLMTKYKLPRPLMFACAHFFTCIGMLFVAFPYPGSIYGASLIIGFG 447

Query: 421 FGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKDGGIKN 480
           FGAQVPM+FAI+SELFGLK+YATIFNCAQLAVPIGSY+LNVDVIG+ YD EA K G  ++
Sbjct: 448 FGAQVPMLFAILSELFGLKYYATIFNCAQLAVPIGSYILNVDVIGKFYDHEATKGGSTRD 507

Query: 481 GKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWIPQSDM 540
           GKGLTC G HCFSGSFL+L+VVVLIG +ASLVLAFRT+DFYKGDVYKKYREDMWIPQSDM
Sbjct: 508 GKGLTCKGTHCFSGSFLVLSVVVLIGGVASLVLAFRTRDFYKGDVYKKYREDMWIPQSDM 567

Query: 541 EFYCLDNKKKVV-NNMPRIVMPPKYSFLNII 571
           EFYCLDNKKKVV NN+PR+VMPPKYSFLNII
Sbjct: 568 EFYCLDNKKKVVNNNLPRVVMPPKYSFLNII 597

[Close]

BLAST of Lsi03G001440 vs. ExPASy TrEMBL
Match: A0A5A7UEF4 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold60G003240 PE=4 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 9.8e-266
Identity = 466/551 (84.57%), Postives = 510/551 (92.56%), Query Frame = 0


Query: 1   MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIV 60
           MIGAGSTYVFGTYSK +KTQF+Y+QT+INTLGFAKDLGSNLGVFAG LGE+APPWVL +V
Sbjct: 28  MIGAGSTYVFGTYSKVIKTQFNYSQTEINTLGFAKDLGSNLGVFAGFLGEIAPPWVLLVV 87

Query: 61  GSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGI 120
           G+ LNFYSYFMIWLSVTRRIAKPQLWQMF YICLAANSQNFANTAVLVTSVRNFPDRRGI
Sbjct: 88  GAFLNFYSYFMIWLSVTRRIAKPQLWQMFFYICLAANSQNFANTAVLVTSVRNFPDRRGI 147

Query: 121 ILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHP 180
           ILGLLKGFVGIGGAILTQF+LA+YGHENP++LVLLL+WFPT +SL F L+IRTINIR+HP
Sbjct: 148 ILGLLKGFVGIGGAILTQFFLALYGHENPSNLVLLLSWFPTLISLAFFLSIRTINIRRHP 207

Query: 181 EELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREEL 240
           EELRVLYHLLYVSIILALFLLFLTITQKQA FSS GY SGAAVIVGLLSIPLLIA+REEL
Sbjct: 208 EELRVLYHLLYVSIILALFLLFLTITQKQAAFSSAGYTSGAAVIVGLLSIPLLIAVREEL 267

Query: 241 VLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKPE 300
           +LFKL  QT+NNPS PVFIPE+K+S+NS   N + SLTP+EEI ETNSP+C SN+F KPE
Sbjct: 268 MLFKLKGQTDNNPSPPVFIPEMKSSSNSTPKN-NESLTPIEEIPETNSPTCLSNVFNKPE 327

Query: 301 RGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVSI 360
           RGED+TILQALFSKDM L+ +GTL GCGSSIAAIDNIGQIGESLGY S+SISIFVSWVSI
Sbjct: 328 RGEDFTILQALFSKDMGLILVGTLFGCGSSIAAIDNIGQIGESLGYTSKSISIFVSWVSI 387

Query: 361 FNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGFG 420
           FNFFGRV SGFISETLMTKYKLPRPLMFA +H  TCIGMLF+AFPY GS+Y ASLIIGFG
Sbjct: 388 FNFFGRVGSGFISETLMTKYKLPRPLMFACAHFFTCIGMLFVAFPYPGSIYGASLIIGFG 447

Query: 421 FGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKDGGIKN 480
           FGAQVPM+FAI+SELFGLK+YATIFNCAQLAVPIGSY+LNVDVIG+ YD EA K G  ++
Sbjct: 448 FGAQVPMLFAILSELFGLKYYATIFNCAQLAVPIGSYILNVDVIGKFYDHEATKGGSTRD 507

Query: 481 GKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWIPQSDM 540
           GKGLTC G HCFSGSFL+L+VVVLIG +ASLVLAFRT+DFYKGDVYKKYREDMWIPQSDM
Sbjct: 508 GKGLTCKGTHCFSGSFLVLSVVVLIGGVASLVLAFRTRDFYKGDVYKKYREDMWIPQSDM 567

Query: 541 EFYCLDNKKKV 552
           EFYCLDNKKK+
Sbjct: 568 EFYCLDNKKKL 577

[Close]

BLAST of Lsi03G001440 vs. ExPASy TrEMBL
Match: A0A6J1HIM2 (uncharacterized protein LOC111464772 OS=Cucurbita moschata OX=3662 GN=LOC111464772 PE=4 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 8.3e-257
Identity = 450/570 (78.95%), Postives = 508/570 (89.12%), Query Frame = 0


Query: 1   MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIV 60
           MIGAGSTY+FGTYSK +K+QFDYNQTQINTLGFAKDLGSN GVFAGLLGEVAPPWVLF+V
Sbjct: 31  MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 90

Query: 61  GSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGI 120
           GS+LNF+SYFMIWLS+T RIAKP  W+MFL+IC+AANSQNFANTA+LVTSVRNFPDRRGI
Sbjct: 91  GSALNFWSYFMIWLSITGRIAKPPFWEMFLFICVAANSQNFANTAILVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHP 180
           ILGLLKGFVG GGAILTQFYLA+YGH+NP +LVLLL+W PT  S++F L+IR I +RK P
Sbjct: 151 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 210

Query: 181 EELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREEL 240
           +ELRVLYHLLYVSIILALFLLFLTITQKQ VF+   Y+SGA VI+GLL IPLLIAIREE 
Sbjct: 211 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 270

Query: 241 VLFKLNKQT-NNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKP 300
           +LFKLNKQT NNNP+ P+ +PE + S  +P T        +++I E  S SCFS  F+KP
Sbjct: 271 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPT--------IQQIPEA-STSCFSMTFKKP 330

Query: 301 ERGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVS 360
           +RGED+TILQALFSKDMALVF+ TL  CG+SIAAIDN+GQ+GESLGYPSQ+ISIFVSWVS
Sbjct: 331 QRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPSQTISIFVSWVS 390

Query: 361 IFNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGF 420
           IFNFFGRV SGFISE LMTKYKLPRPLMFAFSHLLTCIG+LFIAFPY GSVY ASLIIGF
Sbjct: 391 IFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGF 450

Query: 421 GFGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKDGGIK 480
           GFGAQVPMVFAI+SELFGLK+Y+TIFNC QLAVPIGSY+LNVDVIG+LYD EA K GG+K
Sbjct: 451 GFGAQVPMVFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVK 510

Query: 481 NGKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWIPQSD 540
           NGKGLTC G HCFSGSFLILAVVVL+GA+ SLVLA+RT+DFYKGDVY KYREDMWIPQSD
Sbjct: 511 NGKGLTCTGTHCFSGSFLILAVVVLLGAVVSLVLAYRTQDFYKGDVYNKYREDMWIPQSD 570

Query: 541 MEFYCLDNKKKVVNNMPRIVMPPKYSFLNI 570
           MEFYC+D++KK  NN+PR+ MPPKY+FL +
Sbjct: 571 MEFYCIDDRKKGGNNLPRMDMPPKYNFLKL 591

[Close]

BLAST of Lsi03G001440 vs. ExPASy TrEMBL
Match: A0A6J1I3B9 (uncharacterized protein LOC111469246 OS=Cucurbita maxima OX=3661 GN=LOC111469246 PE=4 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 5.4e-256
Identity = 449/570 (78.77%), Postives = 508/570 (89.12%), Query Frame = 0


Query: 1   MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIV 60
           MIGAGSTY+FGTYSK +K+QF+YNQTQINTLGFAKDLGSN GVFAGLLGEVAPPWVLF+V
Sbjct: 31  MIGAGSTYIFGTYSKAIKSQFNYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 90

Query: 61  GSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGI 120
           GS+LNF+SYFMIWLS+T RIAKP  W+MFL+IC+AANSQNFANTA+LVTSVRNFPDRRGI
Sbjct: 91  GSALNFWSYFMIWLSITGRIAKPPFWEMFLFICVAANSQNFANTAILVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHP 180
           ILGLLKGFVG GGAILTQFYLA+YGH+NP +LVLLL+W PT VS++F L+IR I +RKHP
Sbjct: 151 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVVSMLFFLSIRPIKVRKHP 210

Query: 181 EELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREEL 240
           +ELRVLYHLLYVSIILALFLLFLTITQKQ VF+   Y+SGA VI+GLL IPLLIAIREE 
Sbjct: 211 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 270

Query: 241 VLFKLNKQT-NNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKP 300
           +LFKLNKQT NNNP+ P+ +PE + S  +P T        +E+I E  S SCFS  F++P
Sbjct: 271 LLFKLNKQTLNNNPTVPILMPEHEPSPKAPPT--------IEQIPEA-STSCFSMAFKRP 330

Query: 301 ERGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVS 360
           +RGED+TILQALFSKDMALVF+ TL  CG+SIAAIDN+GQ+GESLGYPSQ+ISIFVSWVS
Sbjct: 331 QRGEDFTILQALFSKDMALVFIATLSACGASIAAIDNLGQVGESLGYPSQTISIFVSWVS 390

Query: 361 IFNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGF 420
           IFNFFGRV SGFISE LMTKYKLPRPLMFAFSHLLTCIG+LFIAFPY GSVY ASLIIGF
Sbjct: 391 IFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGF 450

Query: 421 GFGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKDGGIK 480
           GFGAQVPMVFAI+SELFGLK+Y+TIFNC QLAVPIGSY+LNVDVIG+LYD EA K GG+K
Sbjct: 451 GFGAQVPMVFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVK 510

Query: 481 NGKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWIPQSD 540
           NGKGLTC G  CFSGSFLILAVVVL GA+ SLVLA+RT+DFYKGDVY +YREDMWIPQSD
Sbjct: 511 NGKGLTCTGTPCFSGSFLILAVVVLFGAVVSLVLAYRTQDFYKGDVYNRYREDMWIPQSD 570

Query: 541 MEFYCLDNKKKVVNNMPRIVMPPKYSFLNI 570
           MEFYC+D++KK  NN+PR+ MPPKY+FL +
Sbjct: 571 MEFYCIDDRKKGGNNLPRMDMPPKYNFLKL 591

[Close]

BLAST of Lsi03G001440 vs. NCBI nr
Match: XP_038895747.1 (uncharacterized protein LOC120083911 [Benincasa hispida])

HSP 1 Score: 1000.3 bits (2585), Expect = 6.5e-288
Identity = 510/570 (89.47%), Postives = 537/570 (94.21%), Query Frame = 0


Query: 1   MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIV 60
           MIGAGSTYVFGTYS+ MKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEV PPWVLFIV
Sbjct: 31  MIGAGSTYVFGTYSRVMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVVPPWVLFIV 90

Query: 61  GSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGI 120
           GS LNFYSYFMIWLS+T RIAKP+LWQMF YI LAANSQNF+NTAVLVTSVRNFPDRRGI
Sbjct: 91  GSFLNFYSYFMIWLSITCRIAKPKLWQMFFYISLAANSQNFSNTAVLVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHP 180
           ILGLLKGFVGIGGA LTQFYLAIYGHENP +LVLLL+WFPTF+SLVFSLTIRTINI K P
Sbjct: 151 ILGLLKGFVGIGGATLTQFYLAIYGHENPENLVLLLSWFPTFISLVFSLTIRTINIHKSP 210

Query: 181 EELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREEL 240
           EELRVLYHLLYVSIILALFLLFLTITQKQA FS  GYISGAAVI+GLL IPLLIAIREEL
Sbjct: 211 EELRVLYHLLYVSIILALFLLFLTITQKQAAFSPAGYISGAAVIIGLLLIPLLIAIREEL 270

Query: 241 VLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKPE 300
           VLFKLNKQ  N PS PVF+PELKAS+  P TN DT LTPLEEI ETNSPSCFSNIF KPE
Sbjct: 271 VLFKLNKQAENVPSTPVFVPELKASSKPPPTNNDT-LTPLEEIPETNSPSCFSNIFNKPE 330

Query: 301 RGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVSI 360
           RGEDYTILQALFSKDMAL+F+GTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVS VSI
Sbjct: 331 RGEDYTILQALFSKDMALIFIGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSLVSI 390

Query: 361 FNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGFG 420
           FNFFGRV SGFISETLM+KYK+PRPLMFA SHLLTCIGMLF+AFPY GS+Y+ASLIIGFG
Sbjct: 391 FNFFGRVGSGFISETLMSKYKVPRPLMFALSHLLTCIGMLFVAFPYRGSIYIASLIIGFG 450

Query: 421 FGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKDGGIKN 480
           FGAQVPM+FAI+SELFGLK+YATIFNCAQLAVPIGSY+LNV VIG+LYD EA KDGGIKN
Sbjct: 451 FGAQVPMIFAILSELFGLKYYATIFNCAQLAVPIGSYILNVHVIGKLYDVEATKDGGIKN 510

Query: 481 GKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWIPQSDM 540
           GKGLTC GAHCFSGSFL+LAVVVLIGALASLVLAFRTK+FYKGDVYKKY+EDMWIPQSDM
Sbjct: 511 GKGLTCEGAHCFSGSFLVLAVVVLIGALASLVLAFRTKEFYKGDVYKKYKEDMWIPQSDM 570

Query: 541 EFYCLDNKKKVVNNMPRIVMPPKYSFLNII 571
           EFYCLDN+KKVVNN+PRIVMPPKYSFLNII
Sbjct: 571 EFYCLDNRKKVVNNLPRIVMPPKYSFLNII 599

[Close]

BLAST of Lsi03G001440 vs. NCBI nr
Match: XP_008464767.1 (PREDICTED: uncharacterized protein LOC103502570 [Cucumis melo] >TYK17154.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 959.1 bits (2478), Expect = 1.7e-275
Identity = 484/571 (84.76%), Postives = 529/571 (92.64%), Query Frame = 0


Query: 1   MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIV 60
           MIGAGSTYVFGTYSK +KTQF+Y+QT+INTLGFAKDLGSNLGVFAG LGE+APPWVL +V
Sbjct: 28  MIGAGSTYVFGTYSKVIKTQFNYSQTEINTLGFAKDLGSNLGVFAGFLGEIAPPWVLLVV 87

Query: 61  GSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGI 120
           G+ LNFYSYFMIWLSVTRRIAKPQLWQMF YICLAANSQNFANTAVLVTSVRNFPDRRGI
Sbjct: 88  GAFLNFYSYFMIWLSVTRRIAKPQLWQMFFYICLAANSQNFANTAVLVTSVRNFPDRRGI 147

Query: 121 ILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHP 180
           ILGLLKGFVGIGGAILTQF+LA+YGHENP++LVLLL+WFPT +SL F L+IRTINIR+HP
Sbjct: 148 ILGLLKGFVGIGGAILTQFFLALYGHENPSNLVLLLSWFPTLISLAFFLSIRTINIRRHP 207

Query: 181 EELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREEL 240
           EELRVLYHLLYVSIILALFLLFLTITQKQA FSS GY SGAAVIVGLLSIPLLIA+REEL
Sbjct: 208 EELRVLYHLLYVSIILALFLLFLTITQKQAAFSSAGYTSGAAVIVGLLSIPLLIAVREEL 267

Query: 241 VLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKPE 300
           +LFKL  QT+NNPS PVFIPE+K+S+NS   N + SLTP+EEI ETNSP+C SN+F KPE
Sbjct: 268 MLFKLKGQTDNNPSPPVFIPEMKSSSNSTPKN-NESLTPIEEIPETNSPTCLSNVFNKPE 327

Query: 301 RGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVSI 360
           RGED+TILQALFSKDM L+ +GTL GCGSSIAAIDNIGQIGESLGY S+SISIFVSWVSI
Sbjct: 328 RGEDFTILQALFSKDMGLILVGTLFGCGSSIAAIDNIGQIGESLGYTSKSISIFVSWVSI 387

Query: 361 FNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGFG 420
           FNFFGRV SGFISETLMTKYKLPRPLMFA +H  TCIGMLF+AFPY GS+Y ASLIIGFG
Sbjct: 388 FNFFGRVGSGFISETLMTKYKLPRPLMFACAHFFTCIGMLFVAFPYPGSIYGASLIIGFG 447

Query: 421 FGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKDGGIKN 480
           FGAQVPM+FAI+SELFGLK+YATIFNCAQLAVPIGSY+LNVDVIG+ YD EA K G  ++
Sbjct: 448 FGAQVPMLFAILSELFGLKYYATIFNCAQLAVPIGSYILNVDVIGKFYDHEATKGGSTRD 507

Query: 481 GKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWIPQSDM 540
           GKGLTC G HCFSGSFL+L+VVVLIG +ASLVLAFRT+DFYKGDVYKKYREDMWIPQSDM
Sbjct: 508 GKGLTCKGTHCFSGSFLVLSVVVLIGGVASLVLAFRTRDFYKGDVYKKYREDMWIPQSDM 567

Query: 541 EFYCLDNKKKVV-NNMPRIVMPPKYSFLNII 571
           EFYCLDNKKKVV NN+PR+VMPPKYSFLNII
Sbjct: 568 EFYCLDNKKKVVNNNLPRVVMPPKYSFLNII 597

[Close]

BLAST of Lsi03G001440 vs. NCBI nr
Match: XP_004147309.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8652749.1 hypothetical protein Csa_013078 [Cucumis sativus])

HSP 1 Score: 954.1 bits (2465), Expect = 5.3e-274
Identity = 480/571 (84.06%), Postives = 533/571 (93.35%), Query Frame = 0


Query: 1   MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIV 60
           MIGAGSTYVFGTYSK +KTQFDY+QT+INTLGFAKDLGSNLGVFAGLLGE+APPWVLF+V
Sbjct: 27  MIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKDLGSNLGVFAGLLGEIAPPWVLFVV 86

Query: 61  GSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGI 120
           GS LNF+SYFMIWLS+T RIAKPQLWQMF+YICLAANSQNFANTAVLVTSVRNFPDRRGI
Sbjct: 87  GSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICLAANSQNFANTAVLVTSVRNFPDRRGI 146

Query: 121 ILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHP 180
           ILGLLKGFVGIGGAI+TQFYLA++GH+NPASLVLLLAWFPT +S +F L+IRTIN+R+HP
Sbjct: 147 ILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVLLLAWFPTLISSLFFLSIRTINMRRHP 206

Query: 181 EELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREEL 240
           EELRVLYHLLYVSIILALFLLFLT++QKQA FSS GY SGAAVI+GLL +PLLIA+REEL
Sbjct: 207 EELRVLYHLLYVSIILALFLLFLTVSQKQAAFSSAGYASGAAVIIGLLLMPLLIAVREEL 266

Query: 241 VLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKPE 300
           +LFKLN QT+ N S  VF PE+K S++S + N + SL+P+EEI E NSP+C SNI  KPE
Sbjct: 267 MLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKN-NESLSPIEEIPELNSPTCCSNIVNKPE 326

Query: 301 RGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVSI 360
           RGED++ILQALFSKDM L+F+ TLCGCGSSIAAIDNIGQIGESLGYPS+SISIFVSWVSI
Sbjct: 327 RGEDFSILQALFSKDMGLIFVATLCGCGSSIAAIDNIGQIGESLGYPSKSISIFVSWVSI 386

Query: 361 FNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGFG 420
           F+FFGRV SGFISETLMTKYKLPRPLMFAFSHLLTCIGMLF+AFPY GS+YVASL IGFG
Sbjct: 387 FSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFVAFPYPGSIYVASLTIGFG 446

Query: 421 FGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKDGGIKN 480
           FGAQVP++FAI+SELFGLK+YATIFNCAQLAVPIGSYVLNVDVIG+LYD EA KDGGI++
Sbjct: 447 FGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIGSYVLNVDVIGKLYDIEATKDGGIRD 506

Query: 481 GKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWIPQSDM 540
           G GLTC GAHCFSGSFL+LAVVVLIG LASLVLAFRT++FYKGDVYKKYREDMWIPQSDM
Sbjct: 507 GNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAFRTRNFYKGDVYKKYREDMWIPQSDM 566

Query: 541 EFYCLDNKKKVVN-NMPRIVMPPKYSFLNII 571
           EFYCLD+KKKVVN N PRIVMPPKY+FLN+I
Sbjct: 567 EFYCLDDKKKVVNDNFPRIVMPPKYNFLNVI 596

[Close]

BLAST of Lsi03G001440 vs. NCBI nr
Match: KAA0051879.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 925.6 bits (2391), Expect = 2.0e-265
Identity = 466/551 (84.57%), Postives = 510/551 (92.56%), Query Frame = 0


Query: 1   MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIV 60
           MIGAGSTYVFGTYSK +KTQF+Y+QT+INTLGFAKDLGSNLGVFAG LGE+APPWVL +V
Sbjct: 28  MIGAGSTYVFGTYSKVIKTQFNYSQTEINTLGFAKDLGSNLGVFAGFLGEIAPPWVLLVV 87

Query: 61  GSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGI 120
           G+ LNFYSYFMIWLSVTRRIAKPQLWQMF YICLAANSQNFANTAVLVTSVRNFPDRRGI
Sbjct: 88  GAFLNFYSYFMIWLSVTRRIAKPQLWQMFFYICLAANSQNFANTAVLVTSVRNFPDRRGI 147

Query: 121 ILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHP 180
           ILGLLKGFVGIGGAILTQF+LA+YGHENP++LVLLL+WFPT +SL F L+IRTINIR+HP
Sbjct: 148 ILGLLKGFVGIGGAILTQFFLALYGHENPSNLVLLLSWFPTLISLAFFLSIRTINIRRHP 207

Query: 181 EELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREEL 240
           EELRVLYHLLYVSIILALFLLFLTITQKQA FSS GY SGAAVIVGLLSIPLLIA+REEL
Sbjct: 208 EELRVLYHLLYVSIILALFLLFLTITQKQAAFSSAGYTSGAAVIVGLLSIPLLIAVREEL 267

Query: 241 VLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKPE 300
           +LFKL  QT+NNPS PVFIPE+K+S+NS   N + SLTP+EEI ETNSP+C SN+F KPE
Sbjct: 268 MLFKLKGQTDNNPSPPVFIPEMKSSSNSTPKN-NESLTPIEEIPETNSPTCLSNVFNKPE 327

Query: 301 RGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVSI 360
           RGED+TILQALFSKDM L+ +GTL GCGSSIAAIDNIGQIGESLGY S+SISIFVSWVSI
Sbjct: 328 RGEDFTILQALFSKDMGLILVGTLFGCGSSIAAIDNIGQIGESLGYTSKSISIFVSWVSI 387

Query: 361 FNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGFG 420
           FNFFGRV SGFISETLMTKYKLPRPLMFA +H  TCIGMLF+AFPY GS+Y ASLIIGFG
Sbjct: 388 FNFFGRVGSGFISETLMTKYKLPRPLMFACAHFFTCIGMLFVAFPYPGSIYGASLIIGFG 447

Query: 421 FGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKDGGIKN 480
           FGAQVPM+FAI+SELFGLK+YATIFNCAQLAVPIGSY+LNVDVIG+ YD EA K G  ++
Sbjct: 448 FGAQVPMLFAILSELFGLKYYATIFNCAQLAVPIGSYILNVDVIGKFYDHEATKGGSTRD 507

Query: 481 GKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWIPQSDM 540
           GKGLTC G HCFSGSFL+L+VVVLIG +ASLVLAFRT+DFYKGDVYKKYREDMWIPQSDM
Sbjct: 508 GKGLTCKGTHCFSGSFLVLSVVVLIGGVASLVLAFRTRDFYKGDVYKKYREDMWIPQSDM 567

Query: 541 EFYCLDNKKKV 552
           EFYCLDNKKK+
Sbjct: 568 EFYCLDNKKKL 577

[Close]

BLAST of Lsi03G001440 vs. NCBI nr
Match: XP_023521059.1 (uncharacterized protein LOC111784657 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 897.5 bits (2318), Expect = 5.9e-257
Identity = 452/570 (79.30%), Postives = 508/570 (89.12%), Query Frame = 0


Query: 1   MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIV 60
           MIGAGSTY+FGTYSK +K+QFDYNQTQINTLGFAKDLGSN GVFAGLLGEVAPPWVLF+V
Sbjct: 31  MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 90

Query: 61  GSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGI 120
           GS+LNF+SYFMIWLS+T RIAKP  W+MFL+IC+AANSQNFANTA+LVTSVRNFPDRRGI
Sbjct: 91  GSALNFWSYFMIWLSITGRIAKPPFWEMFLFICVAANSQNFANTAILVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHP 180
           ILGLLKGFVG GGAILTQFYLA+YGH+NP +LVLLL+W PT VS++F L+IR I +RKHP
Sbjct: 151 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVVSMLFFLSIRPIKVRKHP 210

Query: 181 EELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREEL 240
           +ELRVLYHLLYVSIILALFLLFLTITQKQ VF+   Y+SGA VI+GLL IPLLIAIREE 
Sbjct: 211 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 270

Query: 241 VLFKLNKQT-NNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKP 300
           +LFKLNKQT NNNP+ P+ +PE + S  +P T        +++I E  S SCFS  F+KP
Sbjct: 271 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPT--------IQQIPEA-STSCFSMAFKKP 330

Query: 301 ERGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVS 360
           +RGED+TILQALFSKDMALVF+ TL  CG+SIAAIDN+GQ+GESLGYPSQSISIFVSWVS
Sbjct: 331 QRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPSQSISIFVSWVS 390

Query: 361 IFNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGF 420
           IFNFFGRV SGFISE LMTKYKLPRPLMFAFSHLLTCIG+LFIAFPY GSVY ASLIIGF
Sbjct: 391 IFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGF 450

Query: 421 GFGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKDGGIK 480
           GFGAQVPMVFAI+SELFGLK+Y+TIFNC QLAVPIGSY+LNVDVIG+LYD EA K GG+K
Sbjct: 451 GFGAQVPMVFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVK 510

Query: 481 NGKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWIPQSD 540
           NGKGLTC G  CFSGSFLILAVVVL GA+ SLVLA+RT+DFYKGDVY KYREDMWIPQSD
Sbjct: 511 NGKGLTCTGTPCFSGSFLILAVVVLFGAVVSLVLAYRTQDFYKGDVYNKYREDMWIPQSD 570

Query: 541 MEFYCLDNKKKVVNNMPRIVMPPKYSFLNI 570
           MEFYC+D++KK  NN+PR+ MPPKY+FL +
Sbjct: 571 MEFYCIDDRKKDGNNLPRMDMPPKYNFLKL 591

[Close]

BLAST of Lsi03G001440 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 568.9 bits (1465), Expect = 4.5e-162
Identity = 299/556 (53.78%), Postives = 391/556 (70.32%), Query Frame = 0


Query: 1   MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIV 60
           M  AG+TY+FGTYSK +K+   Y+QT +N LGF KDLG+N+GV +GL+ EV P W +  +
Sbjct: 28  MACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGVLSGLIAEVTPTWFVLTI 87

Query: 61  GSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGI 120
           GS++NF  YFMIWL+VT ++AKP++WQM LYIC+ ANSQNFANT  LVT V+NFP+ RG+
Sbjct: 88  GSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALVTCVKNFPESRGV 147

Query: 121 ILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHP 180
           +LGLLKG+VG+ GAI TQ Y AIYGH++  SL+LL+AW P  VSLVF   IR   + +  
Sbjct: 148 MLGLLKGYVGLSGAIFTQLYFAIYGHDS-KSLILLIAWLPAAVSLVFVYLIREKKVVRQR 207

Query: 181 EELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREEL 240
            EL V Y  LY+SI LALFL+ + I +KQ  FS   Y + A +   LL +PL +++++EL
Sbjct: 208 NELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLFVPLTVSVKQEL 267

Query: 241 VLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKPE 300
            ++ + K     PS      E+K        ++D          E  + SCFS +F  P 
Sbjct: 268 EVWNMMKLPIEEPS------EVKVEKPKKELDLDQDKAAKVNGEEKETKSCFSTVFSPPP 327

Query: 301 RGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVSI 360
           RGEDYTILQAL S DM ++F+ T CG GSS+ A+DN+GQIGESLGYP+ ++S FVS VSI
Sbjct: 328 RGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTVSSFVSLVSI 387

Query: 361 FNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGFG 420
           +N+FGRV SGF+SE L+ KYKLPRPLM     LL+C G L IAFP  GSVY+AS+++GF 
Sbjct: 388 WNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVYIASILMGFS 447

Query: 421 FGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKD---GG 480
           FGAQ+P++FAI+SELFGLK+Y+T+FNC QLA P+GSY+LNV V G LYD EA+K     G
Sbjct: 448 FGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKEALKQLTARG 507

Query: 481 I--KNGKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWI 540
           +  K+ K LTC G+ C+   FLILA V   GAL SL LA RT++FYKGD+YKK+RE    
Sbjct: 508 LTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDIYKKFRES--- 567

Query: 541 PQSDMEFYCLDNKKKV 552
           P+S+ E    D++K V
Sbjct: 568 PESESEL-VPDSRKAV 572

[Close]

BLAST of Lsi03G001440 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 530.0 bits (1364), Expect = 2.3e-150
Identity = 277/545 (50.83%), Postives = 372/545 (68.26%), Query Frame = 0


Query: 1   MIGAGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIV 60
           M  AG+TY+FG YS  +K    Y+QT +N L F KDLG+N+GV AGLL EV PPW + ++
Sbjct: 32  MSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAGLLNEVTPPWFILLI 91

Query: 61  GSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGI 120
           G+ LNF+ YFMIWL+VT RI+KPQ+W M LYIC+ ANSQ+FANT  LVT V+NFP+ RG+
Sbjct: 92  GAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANTGSLVTCVKNFPESRGV 151

Query: 121 ILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTINIRKHP 180
           +LG+LKG+VG+ GAI+TQ Y A YG E+   L+L++ W P  VS  F  TIR + +++  
Sbjct: 152 VLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAIVSFAFLRTIRIMKVKRQT 211

Query: 181 EELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAIREEL 240
            EL+V Y+ LY+S+ LA FL+ + I  K + F+   +   AAV++ LL +P+++ I EE 
Sbjct: 212 NELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVIVLLLLPIIVVILEEK 271

Query: 241 VLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIFEKPE 300
            L+K  KQ   N  AP+ +   K   +S     D      E + +  +PSC++ +F  PE
Sbjct: 272 KLWK-EKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEKVKTPSCWTTVFNPPE 331

Query: 301 RGEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGYPSQSISIFVSWVSI 360
           RG+DYTILQALFS DM ++F+ T+CG G ++ AIDN+GQIG SLGYP +S+S FVS VSI
Sbjct: 332 RGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGYPKRSVSTFVSLVSI 391

Query: 361 FNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPYSGSVYVASLIIGFG 420
           +N++GRV SG +SE  + KYK PRPLM     LL+C G L IAF   G +YVAS+IIGF 
Sbjct: 392 WNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNVPGGLYVASVIIGFC 451

Query: 421 FGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGRLYDFEAVKD----G 480
           FGAQ P++FAI+SE+FGLK+Y+T++N   +A PIGSY+LNV V G LYD EA K     G
Sbjct: 452 FGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAGYLYDVEAGKQYKALG 511

Query: 481 GIK-NGKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDVYKKYREDMWI 540
             +  G+ L C G  CF  SF+I+A V L G L S+VL  RTK FYK D+YKK+RE    
Sbjct: 512 KTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKFYKSDIYKKFREKALA 571

[Close]

BLAST of Lsi03G001440 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 294.7 bits (753), Expect = 1.6e-79
Identity = 205/543 (37.75%), Postives = 303/543 (55.80%), Query Frame = 0


Query: 4   AGSTYVFGTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLL--------------- 63
           +G++Y FG YS  +K+   Y+Q+ ++T+   KD+G+N GVF+GLL               
Sbjct: 21  SGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSGLLYTYATSNRLRGRGGG 80

Query: 64  -GEVAPPWVLFIVGSSLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVL 123
            G    PWV+  VG+   F  YF+IW SVT  I KP +  M L++ LAA SQ F NTA +
Sbjct: 81  IGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCLFMFLAAQSQTFFNTANV 140

Query: 124 VTSVRNFPDRRGIILGLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVF 183
           V++V NF D  G  +G++KGF+G+ GAIL Q Y  +   + PAS +LLLA  PT +SL+ 
Sbjct: 141 VSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD-PASFILLLAVTPTVLSLLV 200

Query: 184 SLTIRTINIRKHPEELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGL 243
              +R I      ++ + L  L  VS+I+A +L+ + I +     SS   I     ++ +
Sbjct: 201 MPLVR-IYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGLSSWANIVTLVCLLVM 260

Query: 244 LSIPLLIAIREELVLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETN 303
           L++PLLIA R +     + K   ++ S  +  P+   S N              + SE +
Sbjct: 261 LALPLLIARRAQ--RDGMEKTVPHDYSPLISSPKATTSGN--------------QSSEGD 320

Query: 304 SPSCFSNIFEKPERG--EDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLG 363
           S         K E G  E+  +LQA+      L+F+  +CG GS ++ I+NI QIGESL 
Sbjct: 321 S---------KVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLR 380

Query: 364 YPSQSISIFVSWVSIFNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFP 423
           Y S  I+  VS  SI+NF GR  +G+ S+ L+ K   PRPL+ A +     IG L IA  
Sbjct: 381 YSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASG 440

Query: 424 YSGSVYVASLIIGFGFGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIG 483
           + G++YV S+I+G  +G+Q  ++  I SELFG++H  TIFN   +A PIGSY+ +V +IG
Sbjct: 441 FQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIG 500

Query: 484 RLYDFEAVKDGGIKNGKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFYKGDV 529
            +YD  A       +G+G TC G+HCF  SF+I+A V   G L ++VL FRTK  Y+  +
Sbjct: 501 YIYDKTA-------SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYRQIL 529

[Close]

BLAST of Lsi03G001440 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 3.8e-76
Identity = 185/536 (34.51%), Postives = 295/536 (55.04%), Query Frame = 0


Query: 4   AGSTYVF-GTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIVGS 63
           AG  Y+F G+ S  +KT   YNQ QI  LG AK+LG  +G  +G L EV+P WV+ +VG+
Sbjct: 30  AGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGALSEVSPSWVVLLVGA 89

Query: 64  SLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGIIL 123
           + N + Y ++WL VT ++    LW +F+ I +  N + + NTA LV+ + NFP+ RG ++
Sbjct: 90  TQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLVSCIHNFPESRGPVV 149

Query: 124 GLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTI------NI 183
           G+LKGF G+ GAILTQ YL ++   + +S++L++A  P  V L     +R +      N+
Sbjct: 150 GILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPPVVVLALLFIVRPVERSCRTNL 209

Query: 184 RKHPEELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAI 243
           R   ++LR L  +    ++LA++LL L + Q     +     +  A++V  + +P+L+  
Sbjct: 210 RS--DDLRFL-AIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVIFMVVPVLV-- 269

Query: 244 REELVLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIF 303
                           P + VFI          S N  TS+ P E  S  +       + 
Sbjct: 270 ----------------PFSSVFI----------SGNNVTSVKPEEGTSNVDQHEA-RTLI 329

Query: 304 EKPER----------GEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGY 363
           E+ +R          GED+T+LQAL   D  L+FM  + G GS I  IDN+GQI  SLGY
Sbjct: 330 ERSDRPPEKKRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGY 389

Query: 364 PSQSISIFVSWVSIFNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPY 423
              +  IFVS +SI NF GRV  G+ SE ++ K  LPR L  +    +  +G+++ A  +
Sbjct: 390 --SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDW 449

Query: 424 SGSVYVASLIIGFGFGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGR 483
            G +YV +++IG G+GA   +  A VS++FGLK + +++N    A+PIGS+V +  +   
Sbjct: 450 PGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASN 509

Query: 484 LYDFEAVKDGG-IKNGKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFY 522
           +YD+ A K  G     + L C G+ C+S +  +++++ L+  + SL + +RT+ FY
Sbjct: 510 IYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

[Close]

BLAST of Lsi03G001440 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 3.8e-76
Identity = 185/536 (34.51%), Postives = 295/536 (55.04%), Query Frame = 0


Query: 4   AGSTYVF-GTYSKTMKTQFDYNQTQINTLGFAKDLGSNLGVFAGLLGEVAPPWVLFIVGS 63
           AG  Y+F G+ S  +KT   YNQ QI  LG AK+LG  +G  +G L EV+P WV+ +VG+
Sbjct: 30  AGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGALSEVSPSWVVLLVGA 89

Query: 64  SLNFYSYFMIWLSVTRRIAKPQLWQMFLYICLAANSQNFANTAVLVTSVRNFPDRRGIIL 123
           + N + Y ++WL VT ++    LW +F+ I +  N + + NTA LV+ + NFP+ RG ++
Sbjct: 90  TQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLVSCIHNFPESRGPVV 149

Query: 124 GLLKGFVGIGGAILTQFYLAIYGHENPASLVLLLAWFPTFVSLVFSLTIRTI------NI 183
           G+LKGF G+ GAILTQ YL ++   + +S++L++A  P  V L     +R +      N+
Sbjct: 150 GILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPPVVVLALLFIVRPVERSCRTNL 209

Query: 184 RKHPEELRVLYHLLYVSIILALFLLFLTITQKQAVFSSVGYISGAAVIVGLLSIPLLIAI 243
           R   ++LR L  +    ++LA++LL L + Q     +     +  A++V  + +P+L+  
Sbjct: 210 RS--DDLRFL-AIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVIFMVVPVLV-- 269

Query: 244 REELVLFKLNKQTNNNPSAPVFIPELKASTNSPSTNIDTSLTPLEEISETNSPSCFSNIF 303
                           P + VFI          S N  TS+ P E  S  +       + 
Sbjct: 270 ----------------PFSSVFI----------SGNNVTSVKPEEGTSNVDQHEA-RTLI 329

Query: 304 EKPER----------GEDYTILQALFSKDMALVFMGTLCGCGSSIAAIDNIGQIGESLGY 363
           E+ +R          GED+T+LQAL   D  L+FM  + G GS I  IDN+GQI  SLGY
Sbjct: 330 ERSDRPPEKKRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGY 389

Query: 364 PSQSISIFVSWVSIFNFFGRVCSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFIAFPY 423
              +  IFVS +SI NF GRV  G+ SE ++ K  LPR L  +    +  +G+++ A  +
Sbjct: 390 --SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDW 449

Query: 424 SGSVYVASLIIGFGFGAQVPMVFAIVSELFGLKHYATIFNCAQLAVPIGSYVLNVDVIGR 483
            G +YV +++IG G+GA   +  A VS++FGLK + +++N    A+PIGS+V +  +   
Sbjct: 450 PGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASN 509

Query: 484 LYDFEAVKDGG-IKNGKGLTCNGAHCFSGSFLILAVVVLIGALASLVLAFRTKDFY 522
           +YD+ A K  G     + L C G+ C+S +  +++++ L+  + SL + +RT+ FY
Sbjct: 510 IYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

The following BLAST results are available for this feature:

BLAST of Lsi03G001440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	4.6e-26	25.76	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]

BLAST of Lsi03G001440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D1P8	8.0e-276	84.76	Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CMR5	8.0e-276	84.76	uncharacterized protein LOC103502570 OS=Cucumis melo OX=3656 GN=LOC103502570 PE=...	[more]
A0A5A7UEF4	9.8e-266	84.57	Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1HIM2	8.3e-257	78.95	uncharacterized protein LOC111464772 OS=Cucurbita moschata OX=3662 GN=LOC1114647...	[more]
A0A6J1I3B9	5.4e-256	78.77	uncharacterized protein LOC111469246 OS=Cucurbita maxima OX=3661 GN=LOC111469246...	[more]

BLAST of Lsi03G001440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895747.1	6.5e-288	89.47	uncharacterized protein LOC120083911 [Benincasa hispida]	[more]
XP_008464767.1	1.7e-275	84.76	PREDICTED: uncharacterized protein LOC103502570 [Cucumis melo] >TYK17154.1 prote...	[more]
XP_004147309.1	5.3e-274	84.06	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8652749.1 hypothet...	[more]
KAA0051879.1	2.0e-265	84.57	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa]	[more]
XP_023521059.1	5.9e-257	79.30	uncharacterized protein LOC111784657 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi03G001440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28120.1	4.5e-162	53.78	Major facilitator superfamily protein	[more]
AT2G39210.1	2.3e-150	50.83	Major facilitator superfamily protein	[more]
AT1G74780.1	1.6e-79	37.75	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT5G50630.1	3.8e-76	34.51	Major facilitator superfamily protein	[more]
AT5G50520.1	3.8e-76	34.51	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 289..521 e-value: 5.9E-12 score: 47.4
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 3..519
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 4..236 e-value: 1.9E-73 score: 247.0
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 1..532
None	No IPR available	PANTHER	PTHR21576:SF29	PROTEIN NUCLEAR FUSION DEFECTIVE 4	coord: 1..532
None	No IPR available	CDD	cd17354	MFS_Mch1p_like	coord: 1..507 e-value: 9.48622E-92 score: 285.299

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G001440.1	Lsi03G001440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0022857	transmembrane transporter activity
molecular_function	GO:0008270	zinc ion binding