Lsi03G001220 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G001220
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Ef-hand calcium binding protein, putative
Location	chr03: 1726225 .. 1729386 (-)
RNA-Seq Expression	Lsi03G001220
Synteny	Lsi03G001220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATCCTTTCTCTTCGTCCTTTTCCATCTTTTCATTTTGAATCTCACCGAGAATCTGACCCGATAAACACAAAAAACGAAACGAAAGTGGCTTTCTCTGCCACCGTTTCATCCTAAAAGAAGATCCCAGATTCGAATACCCCTCTGATTCTTTGCCCGTTTTGATCCAAATTTTATAACCCATCTCCTTTTTCTTTTTCTTTTTCTTCTCTTCTTTTTGGCCATCAATGGCGTCTTTCTTTGCTAGATTCACTTCCTATACCAATTCCAGTTCCAATTCCAATTCCAGTTCCAATGCCCCATCTGCACCGTCCGCTCCATCGGCGCCGGAAACACAAGGCCACGAACATTCCCATGGAACCGCTTCCCCGTATTACTCCGAACAGCATCGGCCGCAGCCTTATGGGTCTAATTATGGTGGGGTTTCTTCATATGGGTCTTATGGTTTTCCTCCGGAGACCAGCCCTGAGGTAATTAGGAGCTTTCAAATGGTGGACAGGGATGGAAGTGGATTCATCGATGAGAATGAATTGCAGCAAGCCCTTTCTTCTGGCTACCAGAGGTTTAGTCTTAGGACCGTTCGTTTGCTCATCTTCTTGTTCAGAAATCCGGTTGATTCCTCCAGAATGGGTTAGTTTCTTATTTCTTCTCTCCTTTAATCTCCTTGGTGAAAATCAGTACAATCATGCTGGGTTGAAATCGTTTTTCTTTTCACTTTAGACTCTGTTTAATTCTCTCCTGTTCCTGTTCGTCGCCTTTCTTGGATGCTAAATGGGCTGTTTCATCTTATTGTCCTCTCTTTCAAATTAATGAAAATCATGTACATAAACTATTGCCTCGTGGTTGAGTTTGTAGCCAAGTTGAATAGGCAACGATTGGTCTTGTTCGACCTTTACCAAACTAAGGCCAATAGAATGTGAAGAAAAGGAAATTGAAAATGAAAAAGCATGTTTGTTGTTAAATGTGAATACGTTTACAGCTTCATTCTATCTGGATTTCTCAACTGGTTTTAGGCTACTTGTATTTTGCTTGTTTTTAACATATTCATTTCAAGTATTGACTGTTTGCTGGTGCATTTTATTTAACTTTGTGTTACTGAATTGCCTGGTGCAGGGCCTAATGAGTTTACTGCTTTGTGGAACTGTCTTGGCCAATGGCGTGTAAGTAATGTTACTATTGTGTGGCTACCCTCTTTCTCGATCATTTTGCACTCGTAATTCATCTATTTTTGCTCTGACTTCTTACAAAAGACGATGGCAATGAAATTAGAACTTCTGGCTTAAAGTAGACAATTGGTTTTAAATGTCACTATGAACTGTTGTATGGAAAGATAATTATTGGTTGTAATGCTGATCTATAACTTGGGATGGGGTGTTTGAATTCCAACCATAATAAGATTAGATGACAGAAAAGTATTTTAGTTGAACCAATTTGTTGAAGCCATCCGATATATGTGTGCTATCACTAAAGGATTTTTGGAACTTATAGTTCAAAACGTGATTTGTTCTGCATTTTGTTATTTCATTCAGAAAGAAAGACTCTCTTGCTTCTATAGCTACTTGCAGAACAATATAGGAGCACATGCAACATGTATCACTTCTCTTCACTTATAATTGATGCTATTATTATTTTTTTCCTTCCAAATTTTAAGGATCTTTTTAGGATAATGTTTTTTAGAGGCTTGGGGAAAGAGGGTGGATAAGAAGAAAACAAAACAACAGAGCAACCTTTTAATCTAAAAATTCAGAGTTGATTGGCAATTTCATATAGAACATCCTTATAAGGACATTTCCAGGTTGTATATAGCACACATTCTAGTTTTATTTGCCTTTTTCCATTGAAAAATGTTCTTTTTCCTTTTTGTGATTTATTTTTTAGGATAATTAGCTTTGTGCCCTATGAGACTAAGTATGAATTCTGCTAATTTGCAGGGCATGTTTGAGAGATATGATAGAGATAGGAGTGGGAGAATTGATGCATTGGAAATGAGGGATGCACTCTATGGTCTTGGCTATGCAGTTCCATCTTCAGTCCTTCAACTTCTAATTTCACTATACGATGATCGAAGTGGCCAAAGAGTGGAATTTAATTTTGACAGTTTTGTTGAGTAAGTGTTTCTACATAGTTGAATACTTATAGCTTTTTATATGCCAAAAGCCTTAGATACTTACATTTCTGGATTTGTCTGTTTTTGTTGCAGATGTGGCATGATAGTGAAGGTGAGATTGTAGACCTTTTACTCCTTGTTTTTTGAAAGACAGATTCCACTAATATTATCACGTCTACTTGACCATATAATAATATAGATAGATCCTGCCAAAATAATCGAGTGTGGCTTTCTATGTATGAACCAATGCCACGGAGTACCTCTCTTTAAGTAGAACTGCAAGTGTCTATTCATGGTTATCAGAAGTTTTAGTATCATACTGTTAGTAACAGCACATGCTTTCAAATGTGATATCATTTCTAAACCAATGCTTGCAAAATTTCAGGGCTTGACTGAGAAGTTCAAGGAGAAGGATAGGCACTACACGGGTTCGGCGACGCTTACATATGAAGATTTCATGTCAACAATCCTCCCATTTATTGTGTCATACAGTTAAAACCCTCAAAGGTGATAAATATCTCTTCACTTCTTGGATTTTGTGTAATATCATCTCTTCATTTGTTGTTTACTTGAAAATGCTAGAAGCTGTACATTCAAGGGAAGCTGCTGCCAGAAGGTTTATGTTGGCATGGGAGAGTTTCCATGTACAGAGATTTATTTGTTATTTGTTTCTTTCTGTTTTGATTCTGTTGAATAGAAATCTTTGTTTGTTGGTTTTTTAGATTATTTGCGACTGATTTTAATCAAGTTTTGGAACTTGTACTAGAAAGTGAAGGAATATACTGCCCATGGCTTGTTTTGAGGGAGTTCATGCTTTGTTCAATTTTCTTTGTTAGTGGAGCTTGGTTCTGTTCAGTTTTTCGAGTCTTTTTGTCCCCAAATTTAATTGTAGAACTGTAGAAGTGTCACTTGTAAGCTGCTTTAGTGGATTGTCAACTCCTCATTCTTCTGATCTATTTAGCATTTAGAAATTATGTTTGTTGTCAATTGTGTGCGGCCATGAAAAATTTCTGCTACTTATGAGAAAAATTAAGGAAAATCGCTGAAATTGACCCC

mRNA sequence

CAATCCTTTCTCTTCGTCCTTTTCCATCTTTTCATTTTGAATCTCACCGAGAATCTGACCCGATAAACACAAAAAACGAAACGAAAGTGGCTTTCTCTGCCACCGTTTCATCCTAAAAGAAGATCCCAGATTCGAATACCCCTCTGATTCTTTGCCCGTTTTGATCCAAATTTTATAACCCATCTCCTTTTTCTTTTTCTTTTTCTTCTCTTCTTTTTGGCCATCAATGGCGTCTTTCTTTGCTAGATTCACTTCCTATACCAATTCCAGTTCCAATTCCAATTCCAGTTCCAATGCCCCATCTGCACCGTCCGCTCCATCGGCGCCGGAAACACAAGGCCACGAACATTCCCATGGAACCGCTTCCCCGTATTACTCCGAACAGCATCGGCCGCAGCCTTATGGGTCTAATTATGGTGGGGTTTCTTCATATGGGTCTTATGGTTTTCCTCCGGAGACCAGCCCTGAGGTAATTAGGAGCTTTCAAATGGTGGACAGGGATGGAAGTGGATTCATCGATGAGAATGAATTGCAGCAAGCCCTTTCTTCTGGCTACCAGAGGTTTAGTCTTAGGACCGTTCGTTTGCTCATCTTCTTGTTCAGAAATCCGGTTGATTCCTCCAGAATGGGGCCTAATGAGTTTACTGCTTTGTGGAACTGTCTTGGCCAATGGCGTGGCATGTTTGAGAGATATGATAGAGATAGGAGTGGGAGAATTGATGCATTGGAAATGAGGGATGCACTCTATGGTCTTGGCTATGCAGTTCCATCTTCAGTCCTTCAACTTCTAATTTCACTATACGATGATCGAAGTGGCCAAAGAGTGGAATTTAATTTTGACAGTTTTGTTGAATGTGGCATGATAGTGAAGGGCTTGACTGAGAAGTTCAAGGAGAAGGATAGGCACTACACGGGTTCGGCGACGCTTACATATGAAGATTTCATGTCAACAATCCTCCCATTTATTGTGTCATACAGTTAAAACCCTCAAAGAAGCTGTACATTCAAGGGAAGCTGCTGCCAGAAGGTTTATGTTGGCATGGGAGAGTTTCCATGTACAGAGATTTATTTGTTATTTGTTTCTTTCTGTTTTGATTCTGTTGAATAGAAATCTTTGTTTGTTGGTTTTTTAGATTATTTGCGACTGATTTTAATCAAGTTTTGGAACTTGTACTAGAAAGTGAAGGAATATACTGCCCATGGCTTGTTTTGAGGGAGTTCATGCTTTGTTCAATTTTCTTTGTTAGTGGAGCTTGGTTCTGTTCAGTTTTTCGAGTCTTTTTGTCCCCAAATTTAATTGTAGAACTGTAGAAGTGTCACTTGTAAGCTGCTTTAGTGGATTGTCAACTCCTCATTCTTCTGATCTATTTAGCATTTAGAAATTATGTTTGTTGTCAATTGTGTGCGGCCATGAAAAATTTCTGCTACTTATGAGAAAAATTAAGGAAAATCGCTGAAATTGACCCC

Coding sequence (CDS)

ATGGCGTCTTTCTTTGCTAGATTCACTTCCTATACCAATTCCAGTTCCAATTCCAATTCCAGTTCCAATGCCCCATCTGCACCGTCCGCTCCATCGGCGCCGGAAACACAAGGCCACGAACATTCCCATGGAACCGCTTCCCCGTATTACTCCGAACAGCATCGGCCGCAGCCTTATGGGTCTAATTATGGTGGGGTTTCTTCATATGGGTCTTATGGTTTTCCTCCGGAGACCAGCCCTGAGGTAATTAGGAGCTTTCAAATGGTGGACAGGGATGGAAGTGGATTCATCGATGAGAATGAATTGCAGCAAGCCCTTTCTTCTGGCTACCAGAGGTTTAGTCTTAGGACCGTTCGTTTGCTCATCTTCTTGTTCAGAAATCCGGTTGATTCCTCCAGAATGGGGCCTAATGAGTTTACTGCTTTGTGGAACTGTCTTGGCCAATGGCGTGGCATGTTTGAGAGATATGATAGAGATAGGAGTGGGAGAATTGATGCATTGGAAATGAGGGATGCACTCTATGGTCTTGGCTATGCAGTTCCATCTTCAGTCCTTCAACTTCTAATTTCACTATACGATGATCGAAGTGGCCAAAGAGTGGAATTTAATTTTGACAGTTTTGTTGAATGTGGCATGATAGTGAAGGGCTTGACTGAGAAGTTCAAGGAGAAGGATAGGCACTACACGGGTTCGGCGACGCTTACATATGAAGATTTCATGTCAACAATCCTCCCATTTATTGTGTCATACAGTTAA

Protein sequence

MASFFARFTSYTNSSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYGSNYGGVSSYGSYGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAVPSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMSTILPFIVSYS

Homology

[Close]

BLAST of Lsi03G001220 vs. ExPASy Swiss-Prot
Match: Q9ZQH1 (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 PE=2 SV=2)

HSP 1 Score: 229.6 bits (584), Expect = 4.0e-59
Identity = 120/235 (51.06%), Postives = 165/235 (70.21%), Query Frame = 0


Query: 18  SNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYGSNYGGVSSYGSYGFPPE 77
           S S++ APSAP  P +   Q H+        Y S  ++P    S+Y G+       F PE
Sbjct: 2   SYSNAYAPSAPELPESFVQQQHDGESRYTYAYPS--YQPTQQFSSYSGM-------FSPE 61

Query: 78  TSPEVIRSFQMVDRDGSGFIDENELQQALS-SGYQRFSLRTVRLLIFLFRNPVDS-SRMG 137
           T PE++RSF+  DR+ SGF++E+EL+QALS SGY   S RT+RLL+F+++ PVDS  R+G
Sbjct: 62  THPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRLG 121

Query: 138 PNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAVPSSVLQLLISLYDDR 197
           P E+  LWNCL QWR +F RYDRDRSG++++ ++RDA Y LG  +P+SV QL++S +DD 
Sbjct: 122 PKEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDG 181

Query: 198 SGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMSTILPFIVSY 251
           +G+ V+  FDSF+ECGMIVKGLTEKF+E D  YTG ATL+Y+ FM  ++PFI +Y
Sbjct: 182 TGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFIATY 227

[Close]

BLAST of Lsi03G001220 vs. ExPASy Swiss-Prot
Match: Q2QY10 (Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.1e-53
Identity = 117/245 (47.76%), Postives = 154/245 (62.86%), Query Frame = 0


Query: 20  SSSNAP-----SAPSAPSAPETQGHEHSHG---TASPYYSEQHRPQPYGSNYGGVSSYGS 79
           SSS+AP         AP + +  G    +G   ++ PY +    P P  + YG    YGS
Sbjct: 49  SSSSAPYYGGGGGYGAPPSTQPYGSGGGYGAPPSSQPYGAPYGAPPPSSAPYGAPGGYGS 108

Query: 80  -------YGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIFL 139
                    FPP T P V+  FQ  DRDGSG ID+ ELQ ALS   Q FSLRTV LL++L
Sbjct: 109 PFASLVPSAFPPGTDPNVVACFQAADRDGSGMIDDKELQSALSGYSQSFSLRTVHLLMYL 168

Query: 140 FRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAVPSSV 199
           F N  +  ++GP EFT+++  L  WR +FER+DRDRSG+IDA E+RDAL  LGY+V  +V
Sbjct: 169 FTN-TNVRKIGPKEFTSVFYSLQNWRSIFERFDRDRSGKIDATELRDALLSLGYSVSPTV 228

Query: 200 LQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMSTIL 250
           L LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD  ++GSAT TYE FM T+L
Sbjct: 229 LDLLVSKFDKTGGKNKAIEYDNFIECCLTVKGLTEKFKEKDTAFSGSATFTYEAFMLTVL 288

[Close]

BLAST of Lsi03G001220 vs. ExPASy Swiss-Prot
Match: Q9FYE4 (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 4.7e-52
Identity = 121/256 (47.27%), Postives = 157/256 (61.33%), Query Frame = 0


Query: 14  SSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQH------RPQP----YGSNY 73
           S S+   S  A   PS PS     GH   +G   P+    +       P+P    +G  Y
Sbjct: 105 SGSSDYGSYGAGPRPSQPS-----GHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGY 164

Query: 74  GGV---SSYGS-------YGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRF 133
           GG    +SYGS        GF P T P ++  FQ  D+DGSGFID+ ELQ ALSS  QRF
Sbjct: 165 GGYPPQASYGSPFASLIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRF 224

Query: 134 SLRTVRLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDAL 193
           S+RTV LL++LF N  ++ ++GP EFTAL+  L  WR +FER D+DRSGRID  E+RDAL
Sbjct: 225 SMRTVHLLMYLFTNS-NAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDAL 284

Query: 194 YGLGYAVPSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSAT 250
             LG++V   VL LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD  Y+GSAT
Sbjct: 285 LSLGFSVSPVVLDLLVSKFDKSGGKNRAIEYDNFIECCLTVKGLTEKFKEKDTAYSGSAT 344

[Close]

BLAST of Lsi03G001220 vs. ExPASy Swiss-Prot
Match: Q8W4L0 (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.4e-48
Identity = 111/254 (43.70%), Postives = 149/254 (58.66%), Query Frame = 0


Query: 6   ARFTSYTNSSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYGSNYGG 65
           A + S +     ++ SS          AP+  GH   +G A        +   +G  YG 
Sbjct: 90  ASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQSGHGGGYGGAP-------QQSGHGGGYGA 149

Query: 66  VSSYGSYG----------FPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSL 125
                SYG          FPP T P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+
Sbjct: 150 PPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSI 209

Query: 126 RTVRLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYG 185
           RTV LL++LF N  +  ++GP EFT+L+  L  WR +FER+D+DRSGRID  E+RDAL  
Sbjct: 210 RTVHLLMYLFTNS-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMS 269

Query: 186 LGYAVPSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLT 245
           LG++V   +L LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD   +GSA   
Sbjct: 270 LGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIECCLTVKGLTEKFKEKDTALSGSAIFN 329

Query: 246 YEDFMSTILPFIVS 250
           YE+FM T+LPF+V+
Sbjct: 330 YENFMLTVLPFLVA 335

[Close]

BLAST of Lsi03G001220 vs. ExPASy Swiss-Prot
Match: Q5PQ53 (Peflin OS=Xenopus laevis OX=8355 GN=pef1 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 4.3e-29
Identity = 79/221 (35.75%), Postives = 122/221 (55.20%), Query Frame = 0


Query: 22  SNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYGSNYGGVSSYGSYGFPPETSPE 81
           S AP AP  P   +  G  +S   ++PY S+QH     G+  G +        PP   PE
Sbjct: 66  SAAPGAPGGPYGGQAPGGPYSVPGSTPYGSQQHGSYGQGAPAGNI--------PPGVDPE 125

Query: 82  VIRSFQMVDRDGSGFIDENELQQAL-SSGYQRFSLRTVRLLIFLFRNPVDSSRMGPNEFT 141
               FQ VD D SG+I   EL+QAL ++ +  F+  T  +++ +F +  +S R+    F+
Sbjct: 126 AFSWFQTVDTDHSGYISLKELKQALVNTNWSSFNDETCTMMMNMF-DKSNSGRIDMFGFS 185

Query: 142 ALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAVPSSVLQLLISLYDDRSGQRV 201
           ALW  + QWR +F++YDRDRSG I+  E+  AL  +GY +    +Q+++S Y  RS Q  
Sbjct: 186 ALWRFIQQWRNLFQQYDRDRSGSINQGELHQALCQMGYQLSPQFVQIVMSRYAQRSAQ-P 245

Query: 202 EFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMS 242
               D F++    ++ +TE F+EKD    G+A L+YEDF++
Sbjct: 246 GLQLDRFIQICTQLQSMTEAFREKDTGQIGTAKLSYEDFIT 276

[Close]

BLAST of Lsi03G001220 vs. ExPASy TrEMBL
Match: A0A5D3E617 (Putative calcium-binding protein CML48 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003180 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 4.5e-130
Identity = 239/253 (94.47%), Postives = 247/253 (97.63%), Query Frame = 0


Query: 1   MASFFARFTSYTNSSSNSNSSSN--APSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQP 60
           MASFF RFTSY+NSSSNSNS+SN  APSAPSAPSAPETQGHE SH TASPYYSEQH+PQP
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSNSHAPSAPSAPSAPETQGHELSHATASPYYSEQHQPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGSNYGGVSSYGSYGFPP TSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV
Sbjct: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180
           RLLIFLFRNP+DSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240
           AVPSSVLQLLISLYDDRSGQ+VEFNFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYED
Sbjct: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYED 240

Query: 241 FMSTILPFIVSYS 252
           FMSTILPF+VSY+
Sbjct: 241 FMSTILPFLVSYN 253

[Close]

BLAST of Lsi03G001220 vs. ExPASy TrEMBL
Match: A0A1S3CFY8 (probable calcium-binding protein CML48 OS=Cucumis melo OX=3656 GN=LOC103500001 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 4.5e-130
Identity = 239/253 (94.47%), Postives = 247/253 (97.63%), Query Frame = 0


Query: 1   MASFFARFTSYTNSSSNSNSSSN--APSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQP 60
           MASFF RFTSY+NSSSNSNS+SN  APSAPSAPSAPETQGHE SH TASPYYSEQH+PQP
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSNSHAPSAPSAPSAPETQGHELSHATASPYYSEQHQPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGSNYGGVSSYGSYGFPP TSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV
Sbjct: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180
           RLLIFLFRNP+DSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240
           AVPSSVLQLLISLYDDRSGQ+VEFNFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYED
Sbjct: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYED 240

Query: 241 FMSTILPFIVSYS 252
           FMSTILPF+VSY+
Sbjct: 241 FMSTILPFLVSYN 253

[Close]

BLAST of Lsi03G001220 vs. ExPASy TrEMBL
Match: A0A0A0LT26 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G164670 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 4.2e-128
Identity = 234/251 (93.23%), Postives = 244/251 (97.21%), Query Frame = 0


Query: 1   MASFFARFTSYTNSSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYG 60
           MASFF RFTSY+NSSSNSNS+S+APSAPS PSAPETQG EHSH TASPY+SEQ++PQPYG
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSHAPSAPSPPSAPETQGQEHSHATASPYHSEQNQPQPYG 60

Query: 61  SNYGGVSSYGSYGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 120
            NYGGVSSYGSYGFPP TSPEVIRSFQMVDRD SGFIDENELQQALSSGYQRFSLRTVRL
Sbjct: 61  FNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDRSGFIDENELQQALSSGYQRFSLRTVRL 120

Query: 121 LIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV 180
           LIFLFRNP+DSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV
Sbjct: 121 LIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV 180

Query: 181 PSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 240
           PSSVLQLLISLYDDRSGQ+VEFNFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYEDFM
Sbjct: 181 PSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFM 240

Query: 241 STILPFIVSYS 252
           STILPF+VSYS
Sbjct: 241 STILPFLVSYS 251

[Close]

BLAST of Lsi03G001220 vs. ExPASy TrEMBL
Match: A0A6J1HR40 (probable calcium-binding protein CML48 OS=Cucurbita maxima OX=3661 GN=LOC111466605 PE=4 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 3.3e-125
Identity = 228/251 (90.84%), Postives = 241/251 (96.02%), Query Frame = 0


Query: 1   MASFFARFTSYTNSSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYG 60
           MASFF RFTSY+N  SNS+S S+APSAPSAPS PET GH +SHGTASPYYS+QH+PQPYG
Sbjct: 1   MASFFGRFTSYSN--SNSHSHSHAPSAPSAPSEPETYGHGNSHGTASPYYSQQHQPQPYG 60

Query: 61  SNYGGVSSYGSYGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 120
           SNYGGVSSYGSYGFPP TSPEVIRSFQMVD+D SGFIDENELQ+ALSSGYQRFSLRTVRL
Sbjct: 61  SNYGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRL 120

Query: 121 LIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV 180
           LIFLFRNP+DSSRMGPNEFTALWNCLGQWRG FERYDRDRSGRIDALEMRDALYGLGYAV
Sbjct: 121 LIFLFRNPIDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGRIDALEMRDALYGLGYAV 180

Query: 181 PSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 240
           PSSVLQLLISLYDDRSG+RVE+NFDSFVECGMIVKGLTEKFKEKD+HYTGSATLTYEDFM
Sbjct: 181 PSSVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDKHYTGSATLTYEDFM 240

Query: 241 STILPFIVSYS 252
           STILPF+VSYS
Sbjct: 241 STILPFLVSYS 249

[Close]

BLAST of Lsi03G001220 vs. ExPASy TrEMBL
Match: A0A6J1EVZ6 (probable calcium-binding protein CML48 OS=Cucurbita moschata OX=3662 GN=LOC111438517 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.3e-124
Identity = 229/251 (91.24%), Postives = 237/251 (94.42%), Query Frame = 0


Query: 1   MASFFARFTSYTNSSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYG 60
           MASFF RFTS+  SSS+SNS  +APSAP APSAPE  GHEHSHGTASPY+SE H PQPYG
Sbjct: 1   MASFFGRFTSH--SSSSSNSKPHAPSAPYAPSAPEVHGHEHSHGTASPYFSEHHPPQPYG 60

Query: 61  SNYGGVSSYGSYGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 120
           SNYG VSSYGSYGFPP T PEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL
Sbjct: 61  SNYGAVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 120

Query: 121 LIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV 180
           LIFLFRNPVDSSRMGPNEFTALWNCLGQWRG FERYDRDRSG+IDALEMRDALYGLGYAV
Sbjct: 121 LIFLFRNPVDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGKIDALEMRDALYGLGYAV 180

Query: 181 PSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 240
           PSSVL+LLISLYDDRSG+RVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM
Sbjct: 181 PSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 240

Query: 241 STILPFIVSYS 252
           STILPF+VSYS
Sbjct: 241 STILPFLVSYS 249

[Close]

BLAST of Lsi03G001220 vs. NCBI nr
Match: XP_038895549.1 (probable calcium-binding protein CML48 [Benincasa hispida])

HSP 1 Score: 481.5 bits (1238), Expect = 4.5e-132
Identity = 239/251 (95.22%), Postives = 246/251 (98.01%), Query Frame = 0


Query: 1   MASFFARFTSYTNSSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYG 60
           MASFF RFTSY+NS+SNSNS+S+APSAPS PSAPETQGHEHSH TASPYYSEQHRPQPYG
Sbjct: 1   MASFFNRFTSYSNSNSNSNSNSHAPSAPSVPSAPETQGHEHSHATASPYYSEQHRPQPYG 60

Query: 61  SNYGGVSSYGSYGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 120
           SNYGGVSSYGSYGFPP TSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQ+FSLRTVRL
Sbjct: 61  SNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQKFSLRTVRL 120

Query: 121 LIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV 180
           LIFLFRNPVDSSRMGPNEF ALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV
Sbjct: 121 LIFLFRNPVDSSRMGPNEFAALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV 180

Query: 181 PSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 240
           PSSVLQLLISLYDDRSG+RVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM
Sbjct: 181 PSSVLQLLISLYDDRSGERVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 240

Query: 241 STILPFIVSYS 252
           STILPF+VSYS
Sbjct: 241 STILPFLVSYS 251

[Close]

BLAST of Lsi03G001220 vs. NCBI nr
Match: XP_008461406.1 (PREDICTED: probable calcium-binding protein CML48 [Cucumis melo] >KAA0035465.1 putative calcium-binding protein CML48 [Cucumis melo var. makuwa] >TYK31060.1 putative calcium-binding protein CML48 [Cucumis melo var. makuwa])

HSP 1 Score: 473.8 bits (1218), Expect = 9.3e-130
Identity = 239/253 (94.47%), Postives = 247/253 (97.63%), Query Frame = 0


Query: 1   MASFFARFTSYTNSSSNSNSSSN--APSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQP 60
           MASFF RFTSY+NSSSNSNS+SN  APSAPSAPSAPETQGHE SH TASPYYSEQH+PQP
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSNSHAPSAPSAPSAPETQGHELSHATASPYYSEQHQPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGSNYGGVSSYGSYGFPP TSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV
Sbjct: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180
           RLLIFLFRNP+DSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240
           AVPSSVLQLLISLYDDRSGQ+VEFNFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYED
Sbjct: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYED 240

Query: 241 FMSTILPFIVSYS 252
           FMSTILPF+VSY+
Sbjct: 241 FMSTILPFLVSYN 253

[Close]

BLAST of Lsi03G001220 vs. NCBI nr
Match: XP_004139489.1 (probable calcium-binding protein CML48 [Cucumis sativus] >KGN64933.1 hypothetical protein Csa_022705 [Cucumis sativus])

HSP 1 Score: 467.2 bits (1201), Expect = 8.7e-128
Identity = 234/251 (93.23%), Postives = 244/251 (97.21%), Query Frame = 0


Query: 1   MASFFARFTSYTNSSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYG 60
           MASFF RFTSY+NSSSNSNS+S+APSAPS PSAPETQG EHSH TASPY+SEQ++PQPYG
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSHAPSAPSPPSAPETQGQEHSHATASPYHSEQNQPQPYG 60

Query: 61  SNYGGVSSYGSYGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 120
            NYGGVSSYGSYGFPP TSPEVIRSFQMVDRD SGFIDENELQQALSSGYQRFSLRTVRL
Sbjct: 61  FNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDRSGFIDENELQQALSSGYQRFSLRTVRL 120

Query: 121 LIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV 180
           LIFLFRNP+DSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV
Sbjct: 121 LIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV 180

Query: 181 PSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 240
           PSSVLQLLISLYDDRSGQ+VEFNFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYEDFM
Sbjct: 181 PSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYEDFM 240

Query: 241 STILPFIVSYS 252
           STILPF+VSYS
Sbjct: 241 STILPFLVSYS 251

[Close]

BLAST of Lsi03G001220 vs. NCBI nr
Match: KAG6583656.1 (putative calcium-binding protein CML48, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028448.1 putative calcium-binding protein CML48 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 458.0 bits (1177), Expect = 5.3e-125
Identity = 230/251 (91.63%), Postives = 238/251 (94.82%), Query Frame = 0


Query: 1   MASFFARFTSYTNSSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYG 60
           MASFF RFTS+  SSS+SNS  +APSAP APSAPE  GHEHSHGTASPY+SE H PQPYG
Sbjct: 1   MASFFGRFTSH--SSSSSNSKPHAPSAPYAPSAPEVHGHEHSHGTASPYFSEHHPPQPYG 60

Query: 61  SNYGGVSSYGSYGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 120
           SNYGGVSSYGSYGFPP T PEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL
Sbjct: 61  SNYGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 120

Query: 121 LIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV 180
           LIFLFRNPVDSSRMGPNEFTALWNCLGQWRG FERYDRDRSG+IDALEMRDALYGLGYAV
Sbjct: 121 LIFLFRNPVDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGKIDALEMRDALYGLGYAV 180

Query: 181 PSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 240
           PSSVL+LLISLYDDRSG+RVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM
Sbjct: 181 PSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 240

Query: 241 STILPFIVSYS 252
           STILPF+VSYS
Sbjct: 241 STILPFLVSYS 249

[Close]

BLAST of Lsi03G001220 vs. NCBI nr
Match: XP_023519854.1 (probable calcium-binding protein CML48 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 458.0 bits (1177), Expect = 5.3e-125
Identity = 230/251 (91.63%), Postives = 238/251 (94.82%), Query Frame = 0


Query: 1   MASFFARFTSYTNSSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYG 60
           MASFF RFTS+  SSS+SNS  +APSAP APSAPE  GHEHSHGTASPY+SE H PQPYG
Sbjct: 1   MASFFGRFTSH--SSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYG 60

Query: 61  SNYGGVSSYGSYGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 120
           SNYGGVSSYGSYGFPP T PEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL
Sbjct: 61  SNYGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRL 120

Query: 121 LIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAV 180
           LIFLFRNPVDSSRMGPNEFTALWNCLGQWRG FERYDRDRSG+IDALEMRDALYGLGYAV
Sbjct: 121 LIFLFRNPVDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGKIDALEMRDALYGLGYAV 180

Query: 181 PSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 240
           PSSVL+LLISLYDDRSG+RVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM
Sbjct: 181 PSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFM 240

Query: 241 STILPFIVSYS 252
           STILPF+VSYS
Sbjct: 241 STILPFLVSYS 249

[Close]

BLAST of Lsi03G001220 vs. TAIR 10
Match: AT2G27480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 229.6 bits (584), Expect = 2.8e-60
Identity = 120/235 (51.06%), Postives = 165/235 (70.21%), Query Frame = 0


Query: 18  SNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYGSNYGGVSSYGSYGFPPE 77
           S S++ APSAP  P +   Q H+        Y S  ++P    S+Y G+       F PE
Sbjct: 2   SYSNAYAPSAPELPESFVQQQHDGESRYTYAYPS--YQPTQQFSSYSGM-------FSPE 61

Query: 78  TSPEVIRSFQMVDRDGSGFIDENELQQALS-SGYQRFSLRTVRLLIFLFRNPVDS-SRMG 137
           T PE++RSF+  DR+ SGF++E+EL+QALS SGY   S RT+RLL+F+++ PVDS  R+G
Sbjct: 62  THPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRLG 121

Query: 138 PNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAVPSSVLQLLISLYDDR 197
           P E+  LWNCL QWR +F RYDRDRSG++++ ++RDA Y LG  +P+SV QL++S +DD 
Sbjct: 122 PKEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDG 181

Query: 198 SGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMSTILPFIVSY 251
           +G+ V+  FDSF+ECGMIVKGLTEKF+E D  YTG ATL+Y+ FM  ++PFI +Y
Sbjct: 182 TGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFIATY 227

[Close]

BLAST of Lsi03G001220 vs. TAIR 10
Match: AT5G04170.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 206.1 bits (523), Expect = 3.4e-53
Identity = 121/256 (47.27%), Postives = 157/256 (61.33%), Query Frame = 0


Query: 14  SSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQH------RPQP----YGSNY 73
           S S+   S  A   PS PS     GH   +G   P+    +       P+P    +G  Y
Sbjct: 105 SGSSDYGSYGAGPRPSQPS-----GHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGY 164

Query: 74  GGV---SSYGS-------YGFPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRF 133
           GG    +SYGS        GF P T P ++  FQ  D+DGSGFID+ ELQ ALSS  QRF
Sbjct: 165 GGYPPQASYGSPFASLIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRF 224

Query: 134 SLRTVRLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDAL 193
           S+RTV LL++LF N  ++ ++GP EFTAL+  L  WR +FER D+DRSGRID  E+RDAL
Sbjct: 225 SMRTVHLLMYLFTNS-NAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDAL 284

Query: 194 YGLGYAVPSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSAT 250
             LG++V   VL LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD  Y+GSAT
Sbjct: 285 LSLGFSVSPVVLDLLVSKFDKSGGKNRAIEYDNFIECCLTVKGLTEKFKEKDTAYSGSAT 344

[Close]

BLAST of Lsi03G001220 vs. TAIR 10
Match: AT3G10300.3 (Calcium-binding EF-hand family protein )

HSP 1 Score: 193.7 bits (491), Expect = 1.7e-49
Identity = 111/254 (43.70%), Postives = 149/254 (58.66%), Query Frame = 0


Query: 6   ARFTSYTNSSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYGSNYGG 65
           A + S +     ++ SS          AP+  GH   +G A        +   +G  YG 
Sbjct: 90  ASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQSGHGGGYGGAP-------QQSGHGGGYGA 149

Query: 66  VSSYGSYG----------FPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSL 125
                SYG          FPP T P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+
Sbjct: 150 PPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSI 209

Query: 126 RTVRLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYG 185
           RTV LL++LF N  +  ++GP EFT+L+  L  WR +FER+D+DRSGRID  E+RDAL  
Sbjct: 210 RTVHLLMYLFTNS-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMS 269

Query: 186 LGYAVPSSVLQLLISLYDDRSGQRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLT 245
           LG++V   +L LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD   +GSA   
Sbjct: 270 LGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIECCLTVKGLTEKFKEKDTALSGSAIFN 329

Query: 246 YEDFMSTILPFIVS 250
           YE+FM T+LPF+V+
Sbjct: 330 YENFMLTVLPFLVA 335

[Close]

BLAST of Lsi03G001220 vs. TAIR 10
Match: AT3G10300.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 142.1 bits (357), Expect = 6.0e-34
Identity = 86/214 (40.19%), Postives = 118/214 (55.14%), Query Frame = 0


Query: 6   ARFTSYTNSSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYGSNYGG 65
           A + S +     ++ SS          AP+  GH   +G A        +   +G  YG 
Sbjct: 90  ASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQSGHGGGYGGAP-------QQSGHGGGYGA 149

Query: 66  VSSYGSYG----------FPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSL 125
                SYG          FPP T P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+
Sbjct: 150 PPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSI 209

Query: 126 RTVRLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYG 185
           RTV LL++LF N  +  ++GP EFT+L+  L  WR +FER+D+DRSGRID  E+RDAL  
Sbjct: 210 RTVHLLMYLFTNS-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMS 269

Query: 186 LGYAVPSSVLQLLISLYDDRSGQRVEFNFDSFVE 210
           LG++V   +L LL+S +D   G+     +D+F+E
Sbjct: 270 LGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

[Close]

BLAST of Lsi03G001220 vs. TAIR 10
Match: AT3G10300.4 (Calcium-binding EF-hand family protein )

HSP 1 Score: 142.1 bits (357), Expect = 6.0e-34
Identity = 86/214 (40.19%), Postives = 118/214 (55.14%), Query Frame = 0


Query: 6   ARFTSYTNSSSNSNSSSNAPSAPSAPSAPETQGHEHSHGTASPYYSEQHRPQPYGSNYGG 65
           A + S +     ++ SS          AP+  GH   +G A        +   +G  YG 
Sbjct: 90  ASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQSGHGGGYGGAP-------QQSGHGGGYGA 149

Query: 66  VSSYGSYG----------FPPETSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSL 125
                SYG          FPP T P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+
Sbjct: 150 PPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSI 209

Query: 126 RTVRLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYG 185
           RTV LL++LF N  +  ++GP EFT+L+  L  WR +FER+D+DRSGRID  E+RDAL  
Sbjct: 210 RTVHLLMYLFTNS-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMS 269

Query: 186 LGYAVPSSVLQLLISLYDDRSGQRVEFNFDSFVE 210
           LG++V   +L LL+S +D   G+     +D+F+E
Sbjct: 270 LGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

The following BLAST results are available for this feature:

BLAST of Lsi03G001220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQH1	4.0e-59	51.06	Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 ...	[more]
Q2QY10	1.1e-53	47.76	Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2...	[more]
Q9FYE4	4.7e-52	47.27	Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 ...	[more]
Q8W4L0	2.4e-48	43.70	Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 ...	[more]
Q5PQ53	4.3e-29	35.75	Peflin OS=Xenopus laevis OX=8355 GN=pef1 PE=1 SV=1	[more]

BLAST of Lsi03G001220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E617	4.5e-130	94.47	Putative calcium-binding protein CML48 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CFY8	4.5e-130	94.47	probable calcium-binding protein CML48 OS=Cucumis melo OX=3656 GN=LOC103500001 P...	[more]
A0A0A0LT26	4.2e-128	93.23	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G164670 PE=4 SV=1	[more]
A0A6J1HR40	3.3e-125	90.84	probable calcium-binding protein CML48 OS=Cucurbita maxima OX=3661 GN=LOC1114666...	[more]
A0A6J1EVZ6	1.3e-124	91.24	probable calcium-binding protein CML48 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]

BLAST of Lsi03G001220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895549.1	4.5e-132	95.22	probable calcium-binding protein CML48 [Benincasa hispida]	[more]
XP_008461406.1	9.3e-130	94.47	PREDICTED: probable calcium-binding protein CML48 [Cucumis melo] >KAA0035465.1 p...	[more]
XP_004139489.1	8.7e-128	93.23	probable calcium-binding protein CML48 [Cucumis sativus] >KGN64933.1 hypothetica...	[more]
KAG6583656.1	5.3e-125	91.63	putative calcium-binding protein CML48, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023519854.1	5.3e-125	91.63	probable calcium-binding protein CML48 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi03G001220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G27480.1	2.8e-60	51.06	Calcium-binding EF-hand family protein	[more]
AT5G04170.1	3.4e-53	47.27	Calcium-binding EF-hand family protein	[more]
AT3G10300.3	1.7e-49	43.70	Calcium-binding EF-hand family protein	[more]
AT3G10300.2	6.0e-34	40.19	Calcium-binding EF-hand family protein	[more]
AT3G10300.4	6.0e-34	40.19	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 81..109 e-value: 1.9E-4 score: 30.8 coord: 148..176 e-value: 0.032 score: 23.4
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 152..170 e-value: 0.014 score: 14.9
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 82..108 e-value: 1.9E-6 score: 27.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 77..112 score: 12.755995
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 144..179 score: 12.337564
None	No IPR available	GENE3D	1.10.238.10		coord: 69..248 e-value: 3.3E-37 score: 129.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..62
None	No IPR available	PANTHER	PTHR46824:SF2	CALCIUM-BINDING PROTEIN CML48-RELATED	coord: 18..249
None	No IPR available	CDD	cd16180	EFh_PEF_Group_I	coord: 81..246 e-value: 6.30012E-70 score: 210.463
IPR044590	Probable calcium-binding protein CML48/49/50	PANTHER	PTHR46824	CALCIUM-BINDING PROTEIN CML48-RELATED	coord: 18..249
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 90..102
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 157..169
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 72..243

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G001220.1	Lsi03G001220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding