Lsi03G001030 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G001030
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionsubtilisin-like protease SBT3.18
Locationchr03: 1440093 .. 1443488 (+)
RNA-Seq ExpressionLsi03G001030
SyntenyLsi03G001030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTAGCCACTGTGGTGGTGGAAGTGGACATGACTCAAGTACGGTGTGTTGTGGTGGTGTAGCTAGCTAGCTAGGCTGCTATCTTTAAAACCAAAACATCATAATTGAAAAATATAGTCCCGTCAATGTTGTTGCTCCTACCTTACCTTAGCTACATTCTATCAAAATTTAAACTACATAATCTTCCCCATTCCTCAAATTAAATGCCTTGCAACACACGCCATCCCCTTTGCCTCTATTATTTAAACTCTAACTATCCATATTCAAATTTTCATACAATATTGATCAAAATGTTTCCTAAAAATCTTCACTTCTTCTTTTGCCTCTTACTTTCTCTCCTCATTCTTCACTCAACTTCATCATCGATTTCTTCACATGTATTATATAAATACTTCATATATATTCTCTCCCGATATCACTTTGATAACTATTTCGTTTTAATTAGTTTTTGAGTTTTGAATATTCAAATTACAAACCTTGTTATTTATGCGGTGGTTCCTTTGTTTTAATTTGTTATCTACTTTTTAGAAGTATTTAAAAAAAAAAACAAAGCAAGTTGTCAAAACTAAGACCTCGTTTGATAACTATTTAATTTTGCTTATTTACCTCTCAATTTCTTACTATGATTTTCATTGTTAAATTTTAATAACAAAAATAAATTTTTACAAACTACTTTTTTTAGTTTTCAAAACCTAGTATGATTTTGTAAAATGTTGGTAGAAAGTAGATATTAAAGCAAGAAAGTTTAAAAGTAGAAGGGAGTGTTTTATAGACTTAAATTTTTAAAATTAAAAATAAAAAAGGAAAATTTTTAAAAATAGAAAAGTAAGGGAAATTATTTACACAAAATAGCAAAATTTTTGGATAGTTGTGATAGACGCTAATAGAAATATATCAGTATCTATCAGTGATAGAAATGATAGAAGTCTATCAATGTCTATCAGTGTTTTTTTGCTATTTTCTGTAAATAGTTTGATATTTTTTCTATCTGTGAAAATTTTTCAAATAAAAAAACCAAATGTTTACCAAACGGGGCCTAAAAAGTAGTTTTTTTTTTTTTTTTATTTGCTTTTGTCTTTCAGAATTTTGCTAATAATTCAAGTATTTAGTTAGAGAAGATGAAAACTAATAACCATGATAAAAAGTTAGAAAAACAAATACAATTTTCAAAAATCAAATGTCGATCAAATTAAACGGGCCTCAATTTAATTTCTCCCCCTACCCATTACCTAGCTTAAATTACATTACATTACATTTATATGTTTACTCTTATATAGGTGCATATAGTGTACTTAGGGCACAACCGTTTGAGTGATGATACAACTTTGACCTCCAAATACCATCTTCGTCTACTCTCAAAAGTGTTTGCAAGGTTAAATTAATTATATATAATTTAGCTATTCAAGTATTAACTTACTTTATGCATATATATATATATATATATATATTAAATTAATGCATGAATCTAAGTGTTATTGTTGCATGCAGTGAAGAAGATAGTAGAAGAGCCATGTTGTATAGCTACAAGAAAAGTTTTTCGGGGTTTTCTGCGAAGCTTAATGCAAGCCAAGCAATAGCATTATCGAGTAATTATGAAAATTATTAATTAATCAACCTTACTTGGTGATTAAGAATCCGTTTGAATTGACTTTTTAAGTGTTTATAAACATTTTTTTTGCACTTATAAGTACTTATTTAAACACTTAAAAAGTCAATCCAAATGGACCTTAAGATTTTATTAACAATGTGTTTCTGAGTTTTTTTTATTTCCATTGGCATGCAGAAATGGAAGGAGTGATATCTGTATTCGAGAGTAGAACGATGCAACTCCACACAACAAGAAGTTGGGATTTCTTGGGCCTTCCCATTCCCTCATATACTAGTAATATGAAACCATTATTTCCACTTCGCCTTCCTAGCTATGGTGATCACGACGTCGTTGTTGCCATCTTCGATTCAGGTCGTAACAGATCGATTTTGAAATTGTTAAAATTATTTTTTGTTATGTTCAAAATTATTAAGAAATGTGATTATGCAATTTTAAAATCACTTCTAAAACATGTTTTGTCACTTGGGTGTAGTTGTTGAATTTGATAATATTATGTTTGTGGTTTAATTAAAAACTATTTTAGTTCTCGTACGTACTTATATCTTCGGTTCAATTTGGTTCTTGTACTTTTATAATACCCAACTTGGTCCAAGTACTTTCAGGTTTGGTTCAATTTAATCTCTATACTTTTAAAACGTGCTCATCTCTTAATTTCCATTGTAACTTTTTTTCTTTAAATATATATATATATATATATATATATATATATTTTGATCAAATAGTCACTTTTTAAAATACAAAAACCAAAATAAAGTTTTTGAAAATACATGGACCAAATTGAGCTAAATTAAAAAAAAAAAAACACACGATCAAATTGAACGTTGTAAAAGTATATAGACTAGATTAAGAAATAAAAATACAGATTCGTCTCAAACTGTAATTATTTTGTGCAATCCATCCATAGTCTAATTACAATTTTCCACGTAATAAACTTTTTTCCTTTCTTTTTTAAATATTTCTTTTTAAACTTGATTTTAATTGCACACAAATATTATTTAAAGCTTGGTTATTATTAGGTGTGTGGCCGGAATCCAAAAGTTTCGAGGAGAGTGAAGATATGAAGAGAATTCCATGCAATTGGAAAGGTAAATGCGTTAAAGGATACAGGTTCAACCCAGCCTCAGCCTGCAACCGCAAGCTAATTGGCGCTCGCTACTACCTTAAAGGCTTCGAAGCCCAATATGGAGCCCTCAACACCACCGGCAACCCCGAGTTCCACTCCCCTCGAGATTTCTTAGGTCACGGAACTCACACCGCTTCCACAGTGGTCGGTGCCGTTGTACACAATGTCACCTTCACTGCATCTTCATCACTAGCCAAAGGCACTGCCCGAGGCGGGGCTCCCTGGGCTAGGCTGGCGGTGTACAAGGTGTGTTGGGGAAAGGACTACGAAGGGAAGTGCACCGATGTCGACGTCATGGCTGCTTTCGATGATGCTTTGCATGACGGCGTGGACGTCATCTCCGCCTCGTTTGGAGAGACGCCGCCGTTGGCTCCATTGTTCGAGTCGGCCTCGGCTATTGGCTCATTCCATGCGATGCAGCGGGGAGTGAGTGTGGTGTTTTCTGCTGGGAATGATGGACCCCACCCTTCGCTTGTTCAAAATGTGTCTCCTTGGAGTATTTGTGTTGCTGCTTCTACTATGGATCGAACTTTTCCCACTCCAATACGTCTCCACAACTACTTCTCCATCATGGTTATTTTTTTTCTCTTTTAAATGCATCTTAGGCTGCACCTGATCATCTTGTAGTCTACTCATGTGTGAAACTAGATTTTTTTATGTTATAA

mRNA sequence

CCTAGCCACTGTGGTGGTGGAAGTGGACATGACTCAAGTACGGTGCATATAGTGTACTTAGGGCACAACCGTTTGAGTGATGATACAACTTTGACCTCCAAATACCATCTTCGTCTACTCTCAAAAGTGTTTGCAAGTGAAGAAGATAGTAGAAGAGCCATGTTGTATAGCTACAAGAAAAGTTTTTCGGGAACGATGCAACTCCACACAACAAGAAGTTGGGATTTCTTGGGCCTTCCCATTCCCTCATATACTAGTAATATGAAACCATTATTTCCACTTCGCCTTCCTAGCTATGGTGATCACGACGTCGTTGTTGCCATCTTCGATTCAGGTCGTGTGTGGCCGGAATCCAAAAGTTTCGAGGAGAGTGAAGATATGAAGAGAATTCCATGCAATTGGAAAGGTAAATGCGTTAAAGGATACAGGTTCAACCCAGCCTCAGCCTGCAACCGCAAGCTAATTGGCGCTCGCTACTACCTTAAAGGCTTCGAAGCCCAATATGGAGCCCTCAACACCACCGGCAACCCCGAGTTCCACTCCCCTCGAGATTTCTTAGGTCACGGAACTCACACCGCTTCCACAGTGGTCGGTGCCGTTGTACACAATGTCACCTTCACTGCATCTTCATCACTAGCCAAAGGCACTGCCCGAGGCGGGGCTCCCTGGGCTAGGCTGGCGGTGTACAAGGTGTGTTGGGGAAAGGACTACGAAGGGAAGTGCACCGATGTCGACGTCATGGCTGCTTTCGATGATGCTTTGCATGACGGCGTGGACGTCATCTCCGCCTCGTTTGGAGAGACGCCGCCGTTGGCTCCATTGTTCGAGTCGGCCTCGGCTATTGGCTCATTCCATGCGATGCAGCGGGGAGTGAGTGTGGTGTTTTCTGCTGGGAATGATGGACCCCACCCTTCGCTTGTTCAAAATGTGTCTCCTTGGAGTATTTGTGTTGCTGCTTCTACTATGGATCGAACTTTTCCCACTCCAATACGTCTCCACAACTACTTCTCCATCATGGCTGCACCTGATCATCTTGTAGTCTACTCATGTGTGAAACTAGATTTTTTTATGTTATAA

Coding sequence (CDS)

CCTAGCCACTGTGGTGGTGGAAGTGGACATGACTCAAGTACGGTGCATATAGTGTACTTAGGGCACAACCGTTTGAGTGATGATACAACTTTGACCTCCAAATACCATCTTCGTCTACTCTCAAAAGTGTTTGCAAGTGAAGAAGATAGTAGAAGAGCCATGTTGTATAGCTACAAGAAAAGTTTTTCGGGAACGATGCAACTCCACACAACAAGAAGTTGGGATTTCTTGGGCCTTCCCATTCCCTCATATACTAGTAATATGAAACCATTATTTCCACTTCGCCTTCCTAGCTATGGTGATCACGACGTCGTTGTTGCCATCTTCGATTCAGGTCGTGTGTGGCCGGAATCCAAAAGTTTCGAGGAGAGTGAAGATATGAAGAGAATTCCATGCAATTGGAAAGGTAAATGCGTTAAAGGATACAGGTTCAACCCAGCCTCAGCCTGCAACCGCAAGCTAATTGGCGCTCGCTACTACCTTAAAGGCTTCGAAGCCCAATATGGAGCCCTCAACACCACCGGCAACCCCGAGTTCCACTCCCCTCGAGATTTCTTAGGTCACGGAACTCACACCGCTTCCACAGTGGTCGGTGCCGTTGTACACAATGTCACCTTCACTGCATCTTCATCACTAGCCAAAGGCACTGCCCGAGGCGGGGCTCCCTGGGCTAGGCTGGCGGTGTACAAGGTGTGTTGGGGAAAGGACTACGAAGGGAAGTGCACCGATGTCGACGTCATGGCTGCTTTCGATGATGCTTTGCATGACGGCGTGGACGTCATCTCCGCCTCGTTTGGAGAGACGCCGCCGTTGGCTCCATTGTTCGAGTCGGCCTCGGCTATTGGCTCATTCCATGCGATGCAGCGGGGAGTGAGTGTGGTGTTTTCTGCTGGGAATGATGGACCCCACCCTTCGCTTGTTCAAAATGTGTCTCCTTGGAGTATTTGTGTTGCTGCTTCTACTATGGATCGAACTTTTCCCACTCCAATACGTCTCCACAACTACTTCTCCATCATGGCTGCACCTGATCATCTTGTAGTCTACTCATGTGTGAAACTAGATTTTTTTATGTTATAA

Protein sequence

PSHCGGGSGHDSSTVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSGTMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDVVVAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFEAQYGALNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDVDVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSFHAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRLHNYFSIMAAPDHLVVYSCVKLDFFML
Homology
BLAST of Lsi03G001030 vs. ExPASy Swiss-Prot
Match: Q9STQ2 (Subtilisin-like protease SBT3.18 OS=Arabidopsis thaliana OX=3702 GN=SBT3.18 PE=3 SV=2)

HSP 1 Score: 380.9 bits (977), Expect = 1.5e-104
Identity = 194/351 (55.27%), Postives = 250/351 (71.23%), Query Frame = 0

Query: 14  TVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG--------- 73
           TV++VYLG NRL  +  L S +HL LLSKVF S++D+ ++MLYSY   F G         
Sbjct: 29  TVYVVYLGANRLK-NAALASSHHLHLLSKVFTSKDDAEQSMLYSYNNGFLGFSAKLNSTQ 88

Query: 74  -----------------TMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDVVVA 133
                            +++LHTTRSWDFLGL +     N +   P +L +YG  D+VV 
Sbjct: 89  AASLAKLNQVITVFKSKSLKLHTTRSWDFLGLAV----DNARRTPPPQL-AYGS-DIVVG 148

Query: 134 IFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFEAQ 193
           IFD+G +WPES+SF E+ + K IP +W GKCV G  F+P+  CNRKLIGAR+YL+GFE  
Sbjct: 149 IFDTG-IWPESESFRETPEAKPIPSSWNGKCVGGEDFDPSVHCNRKLIGARFYLRGFEET 208

Query: 194 YGALNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWARLA 253
           YG ++ T +PE+ SPRD+LGHGTHTAST VG+VV NV  +    L +GTARGGAP ARLA
Sbjct: 209 YGTIDFTRDPEYRSPRDYLGHGTHTASTAVGSVVRNV--SGFFGLGRGTARGGAPLARLA 268

Query: 254 VYKVCWGKDYEGKCTDVDVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSFHAM 313
           V+K CWGKD EG CT+ D++AAFDDA+HDGV VISASFG +PPL+P FES++ IG+FHA 
Sbjct: 269 VFKTCWGKDLEGVCTEADILAAFDDAIHDGVHVISASFGYSPPLSPFFESSADIGAFHAA 328

Query: 314 QRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRLHNYFSI 339
           +RG+SVVFS GNDGP P +VQNV+PW++ VAAST+DR+FPT I +   F++
Sbjct: 329 ERGISVVFSTGNDGPDPGVVQNVAPWAVSVAASTVDRSFPTRIVIDGSFTL 369

BLAST of Lsi03G001030 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.8e-68
Identity = 144/353 (40.79%), Postives = 210/353 (59.49%), Query Frame = 0

Query: 13  STVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG-------- 72
           S V++VYLG     +  ++T  +H ++L  +  S+E    +++YSY+  FSG        
Sbjct: 27  SKVYVVYLGEKEHDNPESVTESHH-QMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTES 86

Query: 73  ------------------TMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDVVV 132
                               ++ TTR+WD+LG+  P  + ++     L+  + G ++V+V
Sbjct: 87  QAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVS-PGNSDSL-----LQKANMG-YNVIV 146

Query: 133 AIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFEA 192
            + DSG VWPES+ F + +    IP  WKG C  G  FN +  CNRKLIGA+Y++ G  A
Sbjct: 147 GVIDSG-VWPESEMFND-KGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVA 206

Query: 193 QYGALNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWARL 252
           ++G +N T NPE+ SPRDF GHGTH AST+ G+ + NV++     L +GTARGGAP   +
Sbjct: 207 EFGVVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYV---GLGRGTARGGAPGVHI 266

Query: 253 AVYKVCWGKDYEGKCTDVDVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSFHA 312
           AVYK CW     G C+  DV+ A D+A+HDGVD++S S G + PL P  E  S +G+FHA
Sbjct: 267 AVYKACW----SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETEHTS-VGAFHA 326

Query: 313 MQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRLHNYFSIM 340
           + +G+ VV +AGN GP    + NV+PW + VAA+T DR+FPT I L N  +I+
Sbjct: 327 VAKGIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITIL 361

BLAST of Lsi03G001030 vs. ExPASy Swiss-Prot
Match: Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.3e-66
Identity = 155/373 (41.55%), Postives = 207/373 (55.50%), Query Frame = 0

Query: 11  DSSTVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG------ 70
           +   V+IVY G ++         ++H   L  V  SEED+R ++LYSYK S +G      
Sbjct: 22  EEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARASLLYSYKHSINGFAAELT 81

Query: 71  ----------------------TMQLHTTRSWDFLGL-------PIPSYTSNMKPLFP-- 130
                                   + HTTRSW+F+GL        +P   ++    F   
Sbjct: 82  PDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRRKNDADDRFRVG 141

Query: 131 ---LRLPSYGDHDVVVAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASAC 190
              L+   +GD  ++V + DSG VWPESKSF + + M  +P +WKG C  G  FN +S C
Sbjct: 142 RNFLKKAKHGD-GIIVGVLDSG-VWPESKSFND-KGMGPVPKSWKGICQTGVAFN-SSHC 201

Query: 191 NRKLIGARYYLKGFEAQYGALNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASS 250
           NRK+IGARYY+KG+E  YGA N T N +F SPRD  GHG+HTAST VG  V  +  +A  
Sbjct: 202 NRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRRV--LGASALG 261

Query: 251 SLAKGTARGGAPWARLAVYKVCWGKDYEGK-----CTDVDVMAAFDDALHDGVDVISASF 310
             AKG+A GGAP ARLA+YK CW K    K     C + D++AA DDA+ DGV VIS S 
Sbjct: 262 GFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHVISISI 321

Query: 311 GETPPLAPLFESASAIGSFHAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRT 339
           G T P  P  +   A+G+ HA++R + V  SAGN GP P  + N++PW I V AST+DR 
Sbjct: 322 GTTEPF-PFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGASTLDRA 381

BLAST of Lsi03G001030 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.2e-66
Identity = 148/354 (41.81%), Postives = 205/354 (57.91%), Query Frame = 0

Query: 11  DSSTVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG------ 70
           D S VHIVYLG  +  DD    S+ H ++LS +  S+ D+  +M+YSY+  FSG      
Sbjct: 25  DESKVHIVYLGEKQ-HDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGFAAKLT 84

Query: 71  --------------------TMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDV 130
                                 +L TTR+WD+LGL + +      P   L   + GD  V
Sbjct: 85  ESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLSVAN------PNNLLNDTNMGD-QV 144

Query: 131 VVAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGF 190
           ++   D+G VWPES+SF ++  +  IP +WKG C  G +F  ++ CNRKLIGA+Y++ GF
Sbjct: 145 IIGFIDTG-VWPESESFNDN-GVGPIPSHWKGGCESGEKF-ISTNCNRKLIGAKYFINGF 204

Query: 191 EAQYGALNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWA 250
            A+    NTT + ++ S RDF+GHGTHTAS   G+ V N+++     LA G  RGGAP A
Sbjct: 205 LAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISY---KGLAGGNLRGGAPRA 264

Query: 251 RLAVYKVCWGKDYEG--KCTDVDVMAAFDDALHDGVDVISASFGETPPLAPL--FESASA 310
           R+A+YK CW  D  G   C+  D++ A D+++HDGVDV+S S G   PL P        A
Sbjct: 265 RIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIA 324

Query: 311 IGSFHAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRLHN 335
            G+FHA+ +G+ VV + GN GP    V N +PW I VAA+T+DR+FPTPI L N
Sbjct: 325 TGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGN 364

BLAST of Lsi03G001030 vs. ExPASy Swiss-Prot
Match: Q8GUK4 (Subtilisin-like protease SBT3.13 OS=Arabidopsis thaliana OX=3702 GN=SBT3.13 PE=2 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.2e-66
Identity = 150/368 (40.76%), Postives = 208/368 (56.52%), Query Frame = 0

Query: 7   GSGHDSSTVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG-- 66
           G+    S V+IVYLG  R  DD  L +  H ++L  +  S+ED++ +++YSY+  FSG  
Sbjct: 33  GALDSDSKVYIVYLG-EREHDDPELVTASHHQMLESLLQSKEDAQNSLIYSYQHGFSGFA 92

Query: 67  ------------------------TMQLHTTRSWDFLGL-PIPSYTSNMKPLFPLRLPSY 126
                                     +L TTR+WD LGL PIP+  S++  +  L   + 
Sbjct: 93  ALLTSSQAKKISEHPEVIHVIPNRIRKLKTTRAWDHLGLSPIPTSFSSLSSVKGLLHDTN 152

Query: 127 GDHDVVVAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARY 186
              + ++ + DSG +WPESK+  + + +  IP  W+GKC  G +FN    CN KLIGARY
Sbjct: 153 LGSEAIIGVIDSG-IWPESKAVND-QGLGPIPKRWRGKCEPGEQFNATIHCNNKLIGARY 212

Query: 187 YLKGFEAQYGA-LNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTAR 246
           YL G  A  G   N T   +F S RD  GHGTHTA+   G+ V NV++     LA+G  R
Sbjct: 213 YLNGVVAAIGGKFNRTIIQDFQSTRDANGHGTHTATIAGGSFVPNVSY---FGLAQGLVR 272

Query: 247 GGAPWARLAVYKVCW------GKDYEGKCTDVDVMAAFDDALHDGVDVISASFGETPPLA 306
           GGAP AR+A YK CW      G   +G+CT  D+  AFDDA+HDGVDV+S S G   P  
Sbjct: 273 GGAPRARIASYKACWNVMRDEGGGTDGRCTSADMWKAFDDAIHDGVDVLSVSIGGGIPED 332

Query: 307 PLFESASAIGSFHAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRL 341
              +    I +FHA+ +G++VV +AGN+GP    V NV+PW + VAA+T+DR+FPT I L
Sbjct: 333 SEVDKLDYIAAFHAVAKGITVVAAAGNEGPGAHTVDNVAPWLLTVAATTLDRSFPTKITL 392

BLAST of Lsi03G001030 vs. ExPASy TrEMBL
Match: A0A1S3CFU3 (subtilisin-like protease SBT3.18 OS=Cucumis melo OX=3656 GN=LOC103500248 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 6.2e-165
Identity = 295/355 (83.10%), Postives = 305/355 (85.92%), Query Frame = 0

Query: 12  SSTVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG------- 71
           SS V+IVYLGHN L+DD TLTSKYHLRLLSKVFASEED +RAMLYSYKKSFSG       
Sbjct: 28  SSRVYIVYLGHNHLNDDATLTSKYHLRLLSKVFASEEDGKRAMLYSYKKSFSGFSAKLNA 87

Query: 72  -------------------TMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDVV 131
                              TM+LHTTRSWDFLGLPIPSYT+N   LFPLRLPSYGDHDVV
Sbjct: 88  SQAIALSKMEDVISVFESRTMELHTTRSWDFLGLPIPSYTNNRSTLFPLRLPSYGDHDVV 147

Query: 132 VAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFE 191
           V IFDSG VWPESKSF+E+E + RIPCNWKGKCVKGYRFNPASACNRKLIGARYYL GFE
Sbjct: 148 VGIFDSG-VWPESKSFDENEGIGRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLNGFE 207

Query: 192 AQYGALNTTG-NPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWA 251
           AQYGALNTT  NPEF SPRDFLGHGTHTAST VGAVVHNV F  SS LAKGTARGGAPWA
Sbjct: 208 AQYGALNTTAENPEFRSPRDFLGHGTHTASTAVGAVVHNVAFPTSSLLAKGTARGGAPWA 267

Query: 252 RLAVYKVCWGKDYEGKCTDVDVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSF 311
           RLAVYKVCWGKDYEGKCTD DVMAAFDDALHDGVDVISASFGETPPL PLFESASAIGSF
Sbjct: 268 RLAVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFGETPPLTPLFESASAIGSF 327

Query: 312 HAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRLHNYFSIM 340
           HAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPI + N+FSIM
Sbjct: 328 HAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIFILNHFSIM 381

BLAST of Lsi03G001030 vs. ExPASy TrEMBL
Match: A0A0A0LVP7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G153520 PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 8.4e-146
Identity = 269/329 (81.76%), Postives = 275/329 (83.59%), Query Frame = 0

Query: 41  SKVFA---SEEDSRRAMLYSYKKSFSG--------------------------TMQLHTT 100
           SKV A   SEED +RAMLYSYKKSFSG                          TM+LHTT
Sbjct: 5   SKVLAIACSEEDGKRAMLYSYKKSFSGFSAKLNASQAIALSKMEDVISVFESRTMKLHTT 64

Query: 101 RSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDVVVAIFDSGRVWPESKSFEESEDMKRIP 160
           RSWDFLGLPIPSYT+N    F L LPSYGDHDVVVAIFDSG VWPESKSFEESE + RIP
Sbjct: 65  RSWDFLGLPIPSYTNNRSARFSLHLPSYGDHDVVVAIFDSG-VWPESKSFEESEGIGRIP 124

Query: 161 CNWKGKCVKGYRFNPASACNRKLIGARYYLKGFEAQYGALNTTG-NPEFHSPRDFLGHGT 220
           CNWKGKCVKGYRFNPASACNRKLIGARYYLKGFEAQYGALNTT  NPEF SPRDFLGHGT
Sbjct: 125 CNWKGKCVKGYRFNPASACNRKLIGARYYLKGFEAQYGALNTTADNPEFRSPRDFLGHGT 184

Query: 221 HTASTVVGAVVHNVTFTASSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDVDVMAAF 280
           HTAST VGAVVHNV F  SSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTD DVMAAF
Sbjct: 185 HTASTAVGAVVHNVGFPTSSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAF 244

Query: 281 DDALHDGVDVISASFGETPPLAPLFESASAIGSFHAMQRGVSVVFSAGNDGPHPSLVQNV 340
           DDALHDGVDVISASFGE PPL PLFESASAIGSFHAMQRGVSVVFSAGNDG HPSLVQNV
Sbjct: 245 DDALHDGVDVISASFGERPPLIPLFESASAIGSFHAMQRGVSVVFSAGNDGSHPSLVQNV 304

BLAST of Lsi03G001030 vs. ExPASy TrEMBL
Match: A0A5D3CJP0 (Subtilisin-like protease SBT3.18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold315G001050 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.1e-142
Identity = 250/278 (89.93%), Postives = 257/278 (92.45%), Query Frame = 0

Query: 63  SGTMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDVVVAIFDSGRVWPESKSFE 122
           S TM+LHTTRSWDFLGLPIPSYT+N   LFPLRLPSYGDHDVVV IFDSG VWPESKSF+
Sbjct: 10  SRTMELHTTRSWDFLGLPIPSYTNNRSTLFPLRLPSYGDHDVVVGIFDSG-VWPESKSFD 69

Query: 123 ESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFEAQYGALNTTG-NPEFHS 182
           E+E + RIPCNWKGKCVKGYRFNPASACNRKLIGARYYL GFEAQYGALNTT  NPEF S
Sbjct: 70  ENEGIGRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLNGFEAQYGALNTTAENPEFRS 129

Query: 183 PRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWARLAVYKVCWGKDYEGKC 242
           PRDFLGHGTHTAST VGAVVHNV F  SS LAKGTARGGAPWARLAVYKVCWGKDYEGKC
Sbjct: 130 PRDFLGHGTHTASTAVGAVVHNVAFPTSSLLAKGTARGGAPWARLAVYKVCWGKDYEGKC 189

Query: 243 TDVDVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSFHAMQRGVSVVFSAGNDG 302
           TD DVMAAFDDALHDGVDVISASFGETPPL PLFESASAIGSFHAMQRGVSVVFSAGNDG
Sbjct: 190 TDADVMAAFDDALHDGVDVISASFGETPPLTPLFESASAIGSFHAMQRGVSVVFSAGNDG 249

Query: 303 PHPSLVQNVSPWSICVAASTMDRTFPTPIRLHNYFSIM 340
           PHPSLVQNVSPWSICVAASTMDRTFPTPI + N+FSIM
Sbjct: 250 PHPSLVQNVSPWSICVAASTMDRTFPTPIFILNHFSIM 286

BLAST of Lsi03G001030 vs. ExPASy TrEMBL
Match: A0A6J1KZH1 (subtilisin-like protease SBT3.18 OS=Cucurbita maxima OX=3661 GN=LOC111499664 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.5e-134
Identity = 252/354 (71.19%), Postives = 277/354 (78.25%), Query Frame = 0

Query: 12  SSTVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG------- 71
           SS V+IVYLGH  L +D TLTS+ HLRLLSKVFASEED +RAMLYSYK+SFSG       
Sbjct: 21  SSHVYIVYLGHTHL-EDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNA 80

Query: 72  -------------------TMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDVV 131
                              TM+LHTTRSWDFLGLPIPSY+ +  P  P         DVV
Sbjct: 81  SQAMTLSKMEGVISIFMSRTMELHTTRSWDFLGLPIPSYSGSRTPDTPRSRKLADGDDVV 140

Query: 132 VAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFE 191
           V IFDSG +WPES+SFE+ + M  +PC+WKGKCVK YRFNPA ACNRKLIGARYYLKGFE
Sbjct: 141 VGIFDSG-IWPESESFEDDQWMFPVPCSWKGKCVKAYRFNPALACNRKLIGARYYLKGFE 200

Query: 192 AQYGALNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWAR 251
           A+YG LNT+GNPEF SPRDFLGHGTHTAST VG +V +V+FT  SSL KG ARGGAP AR
Sbjct: 201 AEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFT-GSSLGKGIARGGAPRAR 260

Query: 252 LAVYKVCWGKDYEGKCTDVDVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSFH 311
           LAVYKVCWGKDYEGKCTD DVMAAFDDAL DGVDVISASFG +PPLAP  ES+SAIGSFH
Sbjct: 261 LAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFIESSSAIGSFH 320

Query: 312 AMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRLHNYFSIM 340
           AMQ+GVSVVFSAGNDGP+ SLVQNV PWSICVAASTMDRTFPT I + N  SI+
Sbjct: 321 AMQKGVSVVFSAGNDGPYRSLVQNVFPWSICVAASTMDRTFPTQILIQNRISIV 371

BLAST of Lsi03G001030 vs. ExPASy TrEMBL
Match: A0A6J1G7L8 (subtilisin-like protease SBT3.18 OS=Cucurbita moschata OX=3662 GN=LOC111451553 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.6e-133
Identity = 251/354 (70.90%), Postives = 274/354 (77.40%), Query Frame = 0

Query: 12  SSTVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG------- 71
           SS V+IVYLGH RL +D TLTS+ HLRLLSKVF+SEED +RAMLYSYK SFSG       
Sbjct: 21  SSHVYIVYLGHTRL-EDATLTSESHLRLLSKVFSSEEDGKRAMLYSYKHSFSGFSAKLNA 80

Query: 72  -------------------TMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDVV 131
                              TM+LHTTRSWDFLGLPIPSY+       P         DVV
Sbjct: 81  SQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGRRTFDTPRSRKLADGDDVV 140

Query: 132 VAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFE 191
           V IFDSG +WPES+SFE+   M  +PC+WKGKCVK YRFNP+ ACNRKLIGARYYLKGFE
Sbjct: 141 VGIFDSG-IWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFE 200

Query: 192 AQYGALNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWAR 251
           A+YG LNT+GNPEF SPRDFLGHGTHTAST VG +V +V+FT  SSL KG ARGGAP AR
Sbjct: 201 AEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFT-GSSLGKGIARGGAPRAR 260

Query: 252 LAVYKVCWGKDYEGKCTDVDVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSFH 311
           LAVYKVCWGKDYEGKCTD DVMAAFDDAL DGVDVISASFG +PPLAP FES+SAIGSFH
Sbjct: 261 LAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGSFH 320

Query: 312 AMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRLHNYFSIM 340
            MQ+GVSVVFSAGNDGP+ SLVQNV PWSICVAASTMDRTFPT I + N  SIM
Sbjct: 321 GMQKGVSVVFSAGNDGPYRSLVQNVFPWSICVAASTMDRTFPTQILIQNRISIM 371

BLAST of Lsi03G001030 vs. NCBI nr
Match: XP_008461718.2 (PREDICTED: subtilisin-like protease SBT3.18 [Cucumis melo])

HSP 1 Score: 590.1 bits (1520), Expect = 1.3e-164
Identity = 295/355 (83.10%), Postives = 305/355 (85.92%), Query Frame = 0

Query: 12  SSTVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG------- 71
           SS V+IVYLGHN L+DD TLTSKYHLRLLSKVFASEED +RAMLYSYKKSFSG       
Sbjct: 28  SSRVYIVYLGHNHLNDDATLTSKYHLRLLSKVFASEEDGKRAMLYSYKKSFSGFSAKLNA 87

Query: 72  -------------------TMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDVV 131
                              TM+LHTTRSWDFLGLPIPSYT+N   LFPLRLPSYGDHDVV
Sbjct: 88  SQAIALSKMEDVISVFESRTMELHTTRSWDFLGLPIPSYTNNRSTLFPLRLPSYGDHDVV 147

Query: 132 VAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFE 191
           V IFDSG VWPESKSF+E+E + RIPCNWKGKCVKGYRFNPASACNRKLIGARYYL GFE
Sbjct: 148 VGIFDSG-VWPESKSFDENEGIGRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLNGFE 207

Query: 192 AQYGALNTTG-NPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWA 251
           AQYGALNTT  NPEF SPRDFLGHGTHTAST VGAVVHNV F  SS LAKGTARGGAPWA
Sbjct: 208 AQYGALNTTAENPEFRSPRDFLGHGTHTASTAVGAVVHNVAFPTSSLLAKGTARGGAPWA 267

Query: 252 RLAVYKVCWGKDYEGKCTDVDVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSF 311
           RLAVYKVCWGKDYEGKCTD DVMAAFDDALHDGVDVISASFGETPPL PLFESASAIGSF
Sbjct: 268 RLAVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFGETPPLTPLFESASAIGSF 327

Query: 312 HAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRLHNYFSIM 340
           HAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPI + N+FSIM
Sbjct: 328 HAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIFILNHFSIM 381

BLAST of Lsi03G001030 vs. NCBI nr
Match: XP_038894599.1 (subtilisin-like protease SBT3.18 [Benincasa hispida])

HSP 1 Score: 584.3 bits (1505), Expect = 7.0e-163
Identity = 297/355 (83.66%), Postives = 309/355 (87.04%), Query Frame = 0

Query: 12  SSTVHIVYLGHNRLSD-DTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG------ 71
           SS V+IVYLGHN LSD D TLTSKYHL LLSKVFASEEDS+RAMLYSYKK+FSG      
Sbjct: 64  SSHVYIVYLGHNALSDNDATLTSKYHLHLLSKVFASEEDSKRAMLYSYKKAFSGFSAKLN 123

Query: 72  --------------------TMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDV 131
                               TMQLHTTRSWDFLGLPIPS+T+NM+PLFP  LPSYGDHD+
Sbjct: 124 ASQAIALSKMEGVISVFESKTMQLHTTRSWDFLGLPIPSFTNNMRPLFP--LPSYGDHDI 183

Query: 132 VVAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGF 191
           VV IFDSG VWPES+SF+ESE + RIPCNWKGKCVKGYRF+PASACNRKLIGARYYLKGF
Sbjct: 184 VVGIFDSG-VWPESESFKESEGIGRIPCNWKGKCVKGYRFDPASACNRKLIGARYYLKGF 243

Query: 192 EAQYGALNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWA 251
           EAQYGALNTT   EF SPRDFLGHGTHTAST VGAVVHNV FTASSSLAKGTARGGAPWA
Sbjct: 244 EAQYGALNTTAG-EFRSPRDFLGHGTHTASTAVGAVVHNVAFTASSSLAKGTARGGAPWA 303

Query: 252 RLAVYKVCWGKDYEGKCTDVDVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSF 311
           RLAVYKVCWGKDYEGKCTD DVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSF
Sbjct: 304 RLAVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSF 363

Query: 312 HAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRLHNYFSIM 340
           HAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPI L N+FSIM
Sbjct: 364 HAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPILLPNHFSIM 414

BLAST of Lsi03G001030 vs. NCBI nr
Match: XP_031737952.1 (subtilisin-like protease SBT3.18 [Cucumis sativus])

HSP 1 Score: 580.5 bits (1495), Expect = 1.0e-161
Identity = 294/355 (82.82%), Postives = 302/355 (85.07%), Query Frame = 0

Query: 12  SSTVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG------- 71
           SS V+IVYLGHNRL+DD TLTSKYHL LLSKVFASEED +RAMLYSYKKSFSG       
Sbjct: 30  SSHVYIVYLGHNRLNDDATLTSKYHLHLLSKVFASEEDGKRAMLYSYKKSFSGFSAKLNA 89

Query: 72  -------------------TMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDVV 131
                              TM+LHTTRSWDFLGLPIPSYT+N    F L LPSYGDHDVV
Sbjct: 90  SQAIALSKMEDVISVFESRTMKLHTTRSWDFLGLPIPSYTNNRSARFSLHLPSYGDHDVV 149

Query: 132 VAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFE 191
           VAIFDSG VWPESKSFEESE + RIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFE
Sbjct: 150 VAIFDSG-VWPESKSFEESEGIGRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFE 209

Query: 192 AQYGALNTTG-NPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWA 251
           AQYGALNTT  NPEF SPRDFLGHGTHTAST VGAVVHNV F  SSSLAKGTARGGAPWA
Sbjct: 210 AQYGALNTTADNPEFRSPRDFLGHGTHTASTAVGAVVHNVGFPTSSSLAKGTARGGAPWA 269

Query: 252 RLAVYKVCWGKDYEGKCTDVDVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSF 311
           RLAVYKVCWGKDYEGKCTD DVMAAFDDALHDGVDVISASFGE PPL PLFESASAIGSF
Sbjct: 270 RLAVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFGERPPLIPLFESASAIGSF 329

Query: 312 HAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRLHNYFSIM 340
           HAMQRGVSVVFSAGNDG HPSLVQNVSPWSICVAASTMDRTFPT I + N+FSIM
Sbjct: 330 HAMQRGVSVVFSAGNDGSHPSLVQNVSPWSICVAASTMDRTFPTTIFILNHFSIM 383

BLAST of Lsi03G001030 vs. NCBI nr
Match: TYK10626.1 (subtilisin-like protease SBT3.18 [Cucumis melo var. makuwa])

HSP 1 Score: 516.2 bits (1328), Expect = 2.3e-142
Identity = 250/278 (89.93%), Postives = 257/278 (92.45%), Query Frame = 0

Query: 63  SGTMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDVVVAIFDSGRVWPESKSFE 122
           S TM+LHTTRSWDFLGLPIPSYT+N   LFPLRLPSYGDHDVVV IFDSG VWPESKSF+
Sbjct: 10  SRTMELHTTRSWDFLGLPIPSYTNNRSTLFPLRLPSYGDHDVVVGIFDSG-VWPESKSFD 69

Query: 123 ESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFEAQYGALNTTG-NPEFHS 182
           E+E + RIPCNWKGKCVKGYRFNPASACNRKLIGARYYL GFEAQYGALNTT  NPEF S
Sbjct: 70  ENEGIGRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLNGFEAQYGALNTTAENPEFRS 129

Query: 183 PRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWARLAVYKVCWGKDYEGKC 242
           PRDFLGHGTHTAST VGAVVHNV F  SS LAKGTARGGAPWARLAVYKVCWGKDYEGKC
Sbjct: 130 PRDFLGHGTHTASTAVGAVVHNVAFPTSSLLAKGTARGGAPWARLAVYKVCWGKDYEGKC 189

Query: 243 TDVDVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSFHAMQRGVSVVFSAGNDG 302
           TD DVMAAFDDALHDGVDVISASFGETPPL PLFESASAIGSFHAMQRGVSVVFSAGNDG
Sbjct: 190 TDADVMAAFDDALHDGVDVISASFGETPPLTPLFESASAIGSFHAMQRGVSVVFSAGNDG 249

Query: 303 PHPSLVQNVSPWSICVAASTMDRTFPTPIRLHNYFSIM 340
           PHPSLVQNVSPWSICVAASTMDRTFPTPI + N+FSIM
Sbjct: 250 PHPSLVQNVSPWSICVAASTMDRTFPTPIFILNHFSIM 286

BLAST of Lsi03G001030 vs. NCBI nr
Match: KAE8652801.1 (hypothetical protein Csa_022759 [Cucumis sativus])

HSP 1 Score: 513.8 bits (1322), Expect = 1.2e-141
Identity = 259/313 (82.75%), Postives = 264/313 (84.35%), Query Frame = 0

Query: 54  MLYSYKKSFSG--------------------------TMQLHTTRSWDFLGLPIPSYTSN 113
           MLYSYKKSFSG                          TM+LHTTRSWDFLGLPIPSYT+N
Sbjct: 1   MLYSYKKSFSGFSAKLNASQAIALSKMEDVISVFESRTMKLHTTRSWDFLGLPIPSYTNN 60

Query: 114 MKPLFPLRLPSYGDHDVVVAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPA 173
               F L LPSYGDHDVVVAIFDSG VWPESKSFEESE + RIPCNWKGKCVKGYRFNPA
Sbjct: 61  RSARFSLHLPSYGDHDVVVAIFDSG-VWPESKSFEESEGIGRIPCNWKGKCVKGYRFNPA 120

Query: 174 SACNRKLIGARYYLKGFEAQYGALNTTG-NPEFHSPRDFLGHGTHTASTVVGAVVHNVTF 233
           SACNRKLIGARYYLKGFEAQYGALNTT  NPEF SPRDFLGHGTHTAST VGAVVHNV F
Sbjct: 121 SACNRKLIGARYYLKGFEAQYGALNTTADNPEFRSPRDFLGHGTHTASTAVGAVVHNVGF 180

Query: 234 TASSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDVDVMAAFDDALHDGVDVISASFG 293
             SSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTD DVMAAFDDALHDGVDVISASFG
Sbjct: 181 PTSSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFG 240

Query: 294 ETPPLAPLFESASAIGSFHAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTF 340
           E PPL PLFESASAIGSFHAMQRGVSVVFSAGNDG HPSLVQNVSPWSICVAASTMDRTF
Sbjct: 241 ERPPLIPLFESASAIGSFHAMQRGVSVVFSAGNDGSHPSLVQNVSPWSICVAASTMDRTF 300

BLAST of Lsi03G001030 vs. TAIR 10
Match: AT4G26330.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 343.2 bits (879), Expect = 2.5e-94
Identity = 175/332 (52.71%), Postives = 227/332 (68.37%), Query Frame = 0

Query: 46  SEEDSRRAMLYSYKKSFSG--------------------------TMQLHTTRSWDFLGL 105
           S++D+ ++MLYSY   F G                          +++LHTTRSWDFLGL
Sbjct: 13  SKDDAEQSMLYSYNNGFLGFSAKLNSTQAASLAKLNQVITVFKSKSLKLHTTRSWDFLGL 72

Query: 106 PIPSYTSNMKPLFPLRLPSYGDHDVVVAIFDSG-------------RVWPESKSFEESED 165
            +     N +   P +L +YG  D+VV IFD+G              +WPES+SF E+ +
Sbjct: 73  AV----DNARRTPPPQL-AYGS-DIVVGIFDTGLFISLKLLLLSILGIWPESESFRETPE 132

Query: 166 MKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFEAQYGALNTTGNPEFHSPRDFL 225
            K IP +W GKCV G  F+P+  CNRKLIGAR+YL+GFE  YG ++ T +PE+ SPRD+L
Sbjct: 133 AKPIPSSWNGKCVGGEDFDPSVHCNRKLIGARFYLRGFEETYGTIDFTRDPEYRSPRDYL 192

Query: 226 GHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDVDV 285
           GHGTHTAST VG+VV NV  +    L +GTARGGAP ARLAV+K CWGKD EG CT+ D+
Sbjct: 193 GHGTHTASTAVGSVVRNV--SGFFGLGRGTARGGAPLARLAVFKTCWGKDLEGVCTEADI 252

Query: 286 MAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSFHAMQRGVSVVFSAGNDGPHPSL 339
           +AAFDDA+HDGV VISASFG +PPL+P FES++ IG+FHA +RG+SVVFS GNDGP P +
Sbjct: 253 LAAFDDAIHDGVHVISASFGYSPPLSPFFESSADIGAFHAAERGISVVFSTGNDGPDPGV 312

BLAST of Lsi03G001030 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.3e-69
Identity = 144/353 (40.79%), Postives = 210/353 (59.49%), Query Frame = 0

Query: 13  STVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG-------- 72
           S V++VYLG     +  ++T  +H ++L  +  S+E    +++YSY+  FSG        
Sbjct: 27  SKVYVVYLGEKEHDNPESVTESHH-QMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTES 86

Query: 73  ------------------TMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDVVV 132
                               ++ TTR+WD+LG+  P  + ++     L+  + G ++V+V
Sbjct: 87  QAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVS-PGNSDSL-----LQKANMG-YNVIV 146

Query: 133 AIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFEA 192
            + DSG VWPES+ F + +    IP  WKG C  G  FN +  CNRKLIGA+Y++ G  A
Sbjct: 147 GVIDSG-VWPESEMFND-KGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVA 206

Query: 193 QYGALNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWARL 252
           ++G +N T NPE+ SPRDF GHGTH AST+ G+ + NV++     L +GTARGGAP   +
Sbjct: 207 EFGVVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYV---GLGRGTARGGAPGVHI 266

Query: 253 AVYKVCWGKDYEGKCTDVDVMAAFDDALHDGVDVISASFGETPPLAPLFESASAIGSFHA 312
           AVYK CW     G C+  DV+ A D+A+HDGVD++S S G + PL P  E  S +G+FHA
Sbjct: 267 AVYKACW----SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETEHTS-VGAFHA 326

Query: 313 MQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRLHNYFSIM 340
           + +G+ VV +AGN GP    + NV+PW + VAA+T DR+FPT I L N  +I+
Sbjct: 327 VAKGIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITIL 361

BLAST of Lsi03G001030 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 255.0 bits (650), Expect = 9.0e-68
Identity = 155/373 (41.55%), Postives = 207/373 (55.50%), Query Frame = 0

Query: 11  DSSTVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG------ 70
           +   V+IVY G ++         ++H   L  V  SEED+R ++LYSYK S +G      
Sbjct: 22  EEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARASLLYSYKHSINGFAAELT 81

Query: 71  ----------------------TMQLHTTRSWDFLGL-------PIPSYTSNMKPLFP-- 130
                                   + HTTRSW+F+GL        +P   ++    F   
Sbjct: 82  PDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRRKNDADDRFRVG 141

Query: 131 ---LRLPSYGDHDVVVAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASAC 190
              L+   +GD  ++V + DSG VWPESKSF + + M  +P +WKG C  G  FN +S C
Sbjct: 142 RNFLKKAKHGD-GIIVGVLDSG-VWPESKSFND-KGMGPVPKSWKGICQTGVAFN-SSHC 201

Query: 191 NRKLIGARYYLKGFEAQYGALNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASS 250
           NRK+IGARYY+KG+E  YGA N T N +F SPRD  GHG+HTAST VG  V  +  +A  
Sbjct: 202 NRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRRV--LGASALG 261

Query: 251 SLAKGTARGGAPWARLAVYKVCWGKDYEGK-----CTDVDVMAAFDDALHDGVDVISASF 310
             AKG+A GGAP ARLA+YK CW K    K     C + D++AA DDA+ DGV VIS S 
Sbjct: 262 GFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHVISISI 321

Query: 311 GETPPLAPLFESASAIGSFHAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRT 339
           G T P  P  +   A+G+ HA++R + V  SAGN GP P  + N++PW I V AST+DR 
Sbjct: 322 GTTEPF-PFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGASTLDRA 381

BLAST of Lsi03G001030 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 254.2 bits (648), Expect = 1.5e-67
Identity = 148/354 (41.81%), Postives = 205/354 (57.91%), Query Frame = 0

Query: 11  DSSTVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG------ 70
           D S VHIVYLG  +  DD    S+ H ++LS +  S+ D+  +M+YSY+  FSG      
Sbjct: 25  DESKVHIVYLGEKQ-HDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGFAAKLT 84

Query: 71  --------------------TMQLHTTRSWDFLGLPIPSYTSNMKPLFPLRLPSYGDHDV 130
                                 +L TTR+WD+LGL + +      P   L   + GD  V
Sbjct: 85  ESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLSVAN------PNNLLNDTNMGD-QV 144

Query: 131 VVAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGF 190
           ++   D+G VWPES+SF ++  +  IP +WKG C  G +F  ++ CNRKLIGA+Y++ GF
Sbjct: 145 IIGFIDTG-VWPESESFNDN-GVGPIPSHWKGGCESGEKF-ISTNCNRKLIGAKYFINGF 204

Query: 191 EAQYGALNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTARGGAPWA 250
            A+    NTT + ++ S RDF+GHGTHTAS   G+ V N+++     LA G  RGGAP A
Sbjct: 205 LAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISY---KGLAGGNLRGGAPRA 264

Query: 251 RLAVYKVCWGKDYEG--KCTDVDVMAAFDDALHDGVDVISASFGETPPLAPL--FESASA 310
           R+A+YK CW  D  G   C+  D++ A D+++HDGVDV+S S G   PL P        A
Sbjct: 265 RIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIA 324

Query: 311 IGSFHAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRLHN 335
            G+FHA+ +G+ VV + GN GP    V N +PW I VAA+T+DR+FPTPI L N
Sbjct: 325 TGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGN 364

BLAST of Lsi03G001030 vs. TAIR 10
Match: AT4G21650.1 (Subtilase family protein )

HSP 1 Score: 254.2 bits (648), Expect = 1.5e-67
Identity = 150/368 (40.76%), Postives = 208/368 (56.52%), Query Frame = 0

Query: 7   GSGHDSSTVHIVYLGHNRLSDDTTLTSKYHLRLLSKVFASEEDSRRAMLYSYKKSFSG-- 66
           G+    S V+IVYLG  R  DD  L +  H ++L  +  S+ED++ +++YSY+  FSG  
Sbjct: 33  GALDSDSKVYIVYLG-EREHDDPELVTASHHQMLESLLQSKEDAQNSLIYSYQHGFSGFA 92

Query: 67  ------------------------TMQLHTTRSWDFLGL-PIPSYTSNMKPLFPLRLPSY 126
                                     +L TTR+WD LGL PIP+  S++  +  L   + 
Sbjct: 93  ALLTSSQAKKISEHPEVIHVIPNRIRKLKTTRAWDHLGLSPIPTSFSSLSSVKGLLHDTN 152

Query: 127 GDHDVVVAIFDSGRVWPESKSFEESEDMKRIPCNWKGKCVKGYRFNPASACNRKLIGARY 186
              + ++ + DSG +WPESK+  + + +  IP  W+GKC  G +FN    CN KLIGARY
Sbjct: 153 LGSEAIIGVIDSG-IWPESKAVND-QGLGPIPKRWRGKCEPGEQFNATIHCNNKLIGARY 212

Query: 187 YLKGFEAQYGA-LNTTGNPEFHSPRDFLGHGTHTASTVVGAVVHNVTFTASSSLAKGTAR 246
           YL G  A  G   N T   +F S RD  GHGTHTA+   G+ V NV++     LA+G  R
Sbjct: 213 YLNGVVAAIGGKFNRTIIQDFQSTRDANGHGTHTATIAGGSFVPNVSY---FGLAQGLVR 272

Query: 247 GGAPWARLAVYKVCW------GKDYEGKCTDVDVMAAFDDALHDGVDVISASFGETPPLA 306
           GGAP AR+A YK CW      G   +G+CT  D+  AFDDA+HDGVDV+S S G   P  
Sbjct: 273 GGAPRARIASYKACWNVMRDEGGGTDGRCTSADMWKAFDDAIHDGVDVLSVSIGGGIPED 332

Query: 307 PLFESASAIGSFHAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIRL 341
              +    I +FHA+ +G++VV +AGN+GP    V NV+PW + VAA+T+DR+FPT I L
Sbjct: 333 SEVDKLDYIAAFHAVAKGITVVAAAGNEGPGAHTVDNVAPWLLTVAATTLDRSFPTKITL 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9STQ21.5e-10455.27Subtilisin-like protease SBT3.18 OS=Arabidopsis thaliana OX=3702 GN=SBT3.18 PE=3... [more]
Q9ZSB01.8e-6840.79Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 S... [more]
Q9FK761.3e-6641.55Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 S... [more]
Q9MAP72.2e-6641.81Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 S... [more]
Q8GUK42.2e-6640.76Subtilisin-like protease SBT3.13 OS=Arabidopsis thaliana OX=3702 GN=SBT3.13 PE=2... [more]
Match NameE-valueIdentityDescription
A0A1S3CFU36.2e-16583.10subtilisin-like protease SBT3.18 OS=Cucumis melo OX=3656 GN=LOC103500248 PE=3 SV... [more]
A0A0A0LVP78.4e-14681.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G153520 PE=3 SV=1[more]
A0A5D3CJP01.1e-14289.93Subtilisin-like protease SBT3.18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1KZH11.5e-13471.19subtilisin-like protease SBT3.18 OS=Cucurbita maxima OX=3661 GN=LOC111499664 PE=... [more]
A0A6J1G7L81.6e-13370.90subtilisin-like protease SBT3.18 OS=Cucurbita moschata OX=3662 GN=LOC111451553 P... [more]
Match NameE-valueIdentityDescription
XP_008461718.21.3e-16483.10PREDICTED: subtilisin-like protease SBT3.18 [Cucumis melo][more]
XP_038894599.17.0e-16383.66subtilisin-like protease SBT3.18 [Benincasa hispida][more]
XP_031737952.11.0e-16182.82subtilisin-like protease SBT3.18 [Cucumis sativus][more]
TYK10626.12.3e-14289.93subtilisin-like protease SBT3.18 [Cucumis melo var. makuwa][more]
KAE8652801.11.2e-14182.75hypothetical protein Csa_022759 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT4G26330.12.5e-9452.71Subtilisin-like serine endopeptidase family protein [more]
AT4G10520.11.3e-6940.79Subtilase family protein [more]
AT5G45650.19.0e-6841.55subtilase family protein [more]
AT1G32940.11.5e-6741.81Subtilase family protein [more]
AT4G21650.11.5e-6740.76Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 103..322
e-value: 4.6E-79
score: 268.3
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 68..336
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 103..320
e-value: 1.7E-16
score: 60.4
NoneNo IPR availableGENE3D3.50.30.30coord: 323..338
e-value: 4.6E-79
score: 268.3
NoneNo IPR availablePANTHERPTHR10795:SF468SUBTILISIN-LIKE PROTEASE SBT3.18coord: 65..340
NoneNo IPR availablePANTHERPTHR10795:SF468SUBTILISIN-LIKE PROTEASE SBT3.18coord: 11..64
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 73..358
score: 13.155182
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 4..66
e-value: 3.1E-8
score: 36.0
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 65..340
coord: 11..64
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 67..322
e-value: 7.07403E-104
score: 306.062

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G001030.1Lsi03G001030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity