Lsi03G000980 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G000980
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptiontRNA dimethylallyltransferase 2
Locationchr03: 1387996 .. 1396445 (-)
RNA-Seq ExpressionLsi03G000980
SyntenyLsi03G000980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TATAAATAGTCTAAAATTGACTCGTCGGAGAAGAGATGGGCGTCGTTGACGGTCTGCAAATCCGAACATGGGAAGAGAAAAGCCGAAGATGGTGGTGATTATGGGGCCGACTGGTTCTGGGAAGTCCCGTCTCGCTATCGATTTGGCTTCTCACTTCCCCGTCGAGATCATCAACGCCGACTCTATGCAAGTTTATCGAGGATTGGACGTTCTCACAAACAAAGTTCCCCCCGAAGACCAAAATGGTCAGTCTGTCTGTTACTTCTATTTGTATGCTATTCTCTCCAATTGTTGCTGAGACCGTGCTTCCCCTGCTTGCTATTGATTTCTGATTTGGGTTTCGTTATCTAGGAGTGCCGCATCATCTTTTGGGTACAGTGAGTCCGGACGTTGAATTCACGGCTAAACACTTCCGAGATTCTGCCGTTCCTGTAAGTTAGCTACATTGTATCGTTCCAAGGGTTTTTGATTCCAGTGGTTCATTACGCATTCTCAGAGATGTAATTGAAAGAGTTGAAAATTGATGTGTGACATTTTCTATTCCGTTAAAAAGGAAATTAGCGTGGTCGAAAAATGTACTTGTCCTATTCTAGTATAGTTTTCTATTGCTATTCTTCAATGATATGGGTCGATGGGCTTTTATAAGCTTCAGTTCCTGTTTGTAGTCCTGAACATGGTCAATAGTTTCCTCAATTAAGGTCAAGAAGTTAAATCTCTAGAGCAAGCCTGTTATCCTACTGTAGTCGCTGGTCTCATTCTAACGTATAGGAGGCTAGGCCTCTCCTTAGTCCTACACAATACTTGTGGCATATAGGGAAAGGGGAAGAATCAGAGCAAGTCAATAATTAGGTGAATTGGGAAATATTGGTTTTGTACTCGAGTCATAGGAAGAGAAGATGGTTAGTAGTAACTTAATAATTGGCTACACTGGATTTAATAGCAAACATTAGAGTGTTATAAAATACTTGAAGAGTAAGAATGGATAGTGACTTATGGTTTAGAGACCATCTTTTAGGTTAGTAACTCAACAATCCTGTGGTGTGCTGTTTATAGCATATTAATCTTATCGTTTATATGAAGTGTGTGGCTGATTTTTTCCCTTATAATTAATGTTATTTATGTGGTTTTGTCTTGATTCTGCATTTTAATTTCTCAGGTTATTGATGACATAATTTCTCGTGGATGCTTGCCAATGATAGTGGGTGGAACGAATTATTATATACAGGTAAGAAAATGTCCTATTTAGTCATCTAATGGTTAATGGGATAATTTGAGTGCTTCAAGCGGCGGGTATTTTACTGTTGAAATTATTTTAATTGTTATAGTACAAGATTGTTATCATGTAGTTAGAGGAAAATTTTGGACATCGTAAAACTAGTTCTTGACATTTTAGAACGTGTCTCTGGGGATCATCTTCTGCCCTAGTTTTTGGCATTGTACTTAAAATATGATCATTTGATTGGAGAAGTACTAGGTGTAATCAATGTTATTAAAAACATACGATACAGGATTTGTATTGTACAATTTGAATTGAAACGAAGGGCAAATGATTTAAATTGTGGTTTTGAATTAGAAATTACATGTATTGTACAATTCGAGAATTGAATTGTGTGACTTGTTCTTGAATTGAAACATTTCTATTCATGAATCGATAAACCTAAGAAAAAAGGCAAAAGGCCATTTTGAGTTCGTTGCCGACACTGGAGAAGAAAACCTCATGGAGCGGTCGGTGTCAACATAGCGTGGATAACTCATGAAACTAGCATTCTTGTCGGCAATTAGAAGAAAATGGAAAATGAAACTTGAGAGATGAACTGATGAAGATCGTGGAAGAAGAAAGAAGAGGAAGGGCTGCTGTCGTGTTTCTCAATTTCCATTGAAGGAGAAAGAGTCTCAAGGAAAATGAAAAGACTATTGATTTGAGATGCTTTGATGTCTGGAACAAAATCTTTATTATAAATTAAAGTCCAAAGTATTAAAATATCATTTAAACATGCACTAAGCACTCAAATCAATAGTCTTTTGATTTTCCTTTTGACTCTCTTTCTTCTTCAATGGAAATTGGGAAACACGATTGCAACCCTTCCCCTTCTTTCTTCTTCTACATTCTTCATCAGTTCATCTCTCAAGTTTTGTTTTCCATTTTCTTCTAATTGCCAACAAGAACGCTTGTTTCATGATTTTCTATCCACATTACATTGTCGCCGACCGCTCCATGAGGTTTTCTTCTTTGGCACTGGCAAACGGTATTTGGCCTTTTATTTATGTACTTTTTTTTAGGTTTATTGATTTTTGAATCAGAACGTTCCAATTCAAGAACGATTCGCACGATTCAGTATCTCGAATCATACGATACATGTAATTTCCACTTCAAAACGACGATTTGAATGGTTTGTCCTTTGTTTCAATTCGAATTGTTAAAAACACTGTGTATCTTCCTTTGGGAGATTTGAACTTCATGTTTAACTTCTAATCGCCCTTTTTTGTGGACTTTGCTTATTTATATATCATTTTATTACGTCAAGCTACCATGATTTCTCATGTAGGCTCTTGTTAGCCCGTTTCTTCTGGATGATTCTGCGGAAACTTTTGGTGGAAGTTGCTCCATTTATCCTGGTAATGACTAATTCTACAGGTTCACGTTTAATAGTATTCATATGGATCTGGTGTTTAAATTAAATTTTTTTATTCATGTTTTTCCAGGAGATGAAGAGCTGGGTATTGAATTGGATGAGGAAAGAGATGGTTTGAAGTATGATCATAACCATCTTAAAGAGATTGATCCAGTTTCGGCTAACAGAATCCATCCTAATAACCACCGAAAAGTAAGGCTGCCAGTTTATGTTATGTGTAATCTGTTAGGAACCAAGGGTAGTATGGGCTACCTTTGTCCCATATTGGTTAGAATGGGATGACCAATGTGGTACTTAAGTGGCTTGGCTCTCCCACCTCAATAGCTAGCTTTTGGGGTGTGGTTCTCCAAGGTACTTAAGTACCTAACAATGGTATTAGAGCCGGTTGTTGACGTTCCGGAGTGGAACCAACGGAGGGTTCTGACCGGGTGTGGCCGAGTGAAGTACTGTTGGAGTAGCCGCCGGGACGTCGGCTTTCCGGGGATAGGGTATTGTTAGGAACCAAGGGTAGTATGGCTACCTTTGTCCCACATTGGTTAGAATGGATGACTGGCTTGGCTCTCCCACCTCAATGTGGTACTTAAGTGGCTTGGCTCTCCCACCTCCATGTGGTACTTAAGTGGCTTGGCTCGCCCACCTCAATAGCTAGCTTTTGGGGTGTGGTTCCCCAAGGTACTTAAGTACCTAACAATGGTATTAGAGCCGGTTGTTGACGTTCCGGAGTGGAACCAACGGAGGGTTCTGACCGGGTGTGGCCGAGTGAAGTACTGTTGGAGTAGCCGCCGGGACGTCGACTTCCCGGGGATAGGGTATTATTAGGAACCAAGGGTAGTAGGGGCTACCTTTGTCCCATATTGGTTAGAATGGGCTGACCAATGTGGTACTTAGGTGGCTTGGCTCTCCCACCTCCATGTGGTACTTAAGTGGCTTGGCTCGCCCACCTCAATAGCTAGCTTTGGGGGTGTGGTTCTCCACGGTACTTAAGTACCACATTGAGGGGGGAGAGCCAAGCCAGTCATCCATGCTAACCAATGTGGGACAAAGGTAGCCCCTACTACCCTTGGTTCCTAATAATACCCTATCCCCGGGAAGTCGACGTCCCGGCGGCTACTCCAACAGTACTTCACTCGGCCACACCCGGTCAGAACCCTCCGTTGGTTCCACTCCAGAACGTCAACAACCGGCTCTAATACCATTGTTAGGTACTTAAGTACCTAACAATGGTATTAGAGCCGGTTGTTGACGTTCCGGAGTGGAACCAACGGAGGGTTCTGACCGGGTGTGGCCGAGTGAAGTACTGTTGGAGTAGCCGCCGGGACGTCGACTTCCCGGGGATAGGGTATTATTAGGAACCAAGGGTAGTAGGGGCTACCTTTGTCCCATATTGGTTAGAATGGGCTGACCAATGTGGTACTTAGGTGGCTTGGCTCTCCCACCTCCATGTGGTACTTAAGTACCGTGGAGAACCACACCCCAAAAGCTAGCTATTGAGGTGGGAGAGCCAAGCCACTTAAGTACCACATTGGTCATCCCATTCTAACCAATGTGGGACAAAGGTAGTCCATACTACCCTAAGTTCCTAACATAATCCTTCTTATAAATGTCAAAACATGTAAAGTCATAGTTTTTGAAACTTGAACAATTGATTTGAATTTCAATGTTATTCTAGTGGATAACATATGGTTCTTAAGGGTGGGCTATCTTTAATTATAGTTGCCAAATTGTAGAATAATGCAAATTGTCTCTAAACATGAGTGGTAGAGAACCAGAGTTTTACATGAAATTTTGAATGCCATCTGGTCTGTGTGATCTTAGTGGCTTCTCTAGTGTCTGGATGTTTATGGTATGTTTTGTCTAATGTATCCTTTAGAATCGTTGCAGAGTTTCTTGCATTCACATTTTTCATTCGAGAGGCATTGGACACCTTATACTTTTTCCTCTTGTTCATATTTTTTTGTAGTTAAGTTTTTCATATCAGTAAATATGCGTTGAATCTGTGTACTTCTGTGAGTTCCCTCTTCGTTTTGTTTATGTTTTAAAAGAAGTGTAAATACGATGTCTTTCTGGGGGCCAGTCTATGACCTACTTTGCGGAAGTTTGGGACTTCATTGGATTGTTCTCAAGTTAAGTAACCAGATTGAAAAATTGAGGCACATGTTGTATTGGGAGACAGTTGGGTTGGAATGCATTGGAGTTGAGCATTACAAAATGGTTATTGTACTTTCCCCTTAGTCTACTTCCTTGTGGAATAGGATAATTGTGAGCCAGGCTTTATTGCCCTTTTGTAATTTAAGGATCTTACTGCTCTTATAATACACTGGAATAGAAATGTGTAATCTCTCGTGGCTTTATTTCACTTTTGTAATTTCACATTATCAATGAAATTATGATTGATCGTTTCTCATCAATGGAAAAAATGGATAATTGTGAGCAAGTACGATTCTCATCTTTTGATTGGATGACGGGCGAGGACAACAAACCTTTCAAAAATATGTGGAAAGAAATTTGTAAGAAGCCCCCTTGTTTCCTTTCATTTTGTACTCTATAATTTGGAGAATAATCTAATTGTATTATTCATTAGACTTAAGATACATCTATATATATATATAGGCGAGATGGAAACCCTAATCTAGAGAATGTGAAATTACAATAAAGGACACTGTACAGTAAATGCATATTATAACACTCCCCCTCAAGCTAGAGCAAATATGTCAATCATGCCCAGCTTGTTACATAGATAGTTTATTCTTGCTCCGTTTACATCTTTGGTAAGGATATCTCCCAACTGTTCTCCAGTCTTCACATATCCTGTAGATATCACCCCTTCTTGTATTTTCTCGCGAATGAAATGACAATTCACTTCAATATGTTTAGTTCGTTCATGAAACACTGGGTTGGATGCAATGTGAAGTGCAGCTTGGTTATCACATCATAATTTAGCTGGCACGGTAACGCTGAAGCCCATCTCAGATAAAAGTTGGTGTATCCACATTATTTCACTCATAGATTGTGTCATATCAGTACTAAGTAAGTAAAACTGAATCGTTCGTCGCCAATCATAGTAATTAGATCCATTTAACTTGTGTTCTGTGATCTTTGATGTTAAGGGAATGACATTAGACACCACCATATGCTTCGGGTCAGCCATGATGAATTAACACACAATTCGTAGATCTCGCAAGCGGAATAAACGCAAGAGGAAGGTGAACCAAAGAGCCAAAATAACAAAACAGCCCCAAAATAGACCAAAACAGTCACAGACCCTAAGAACAAAAAGGATATTTTCCAAATAACCTTACACCCGAGTGGATTTCGATGAAACCAAATGCACAGACAAGTTGGAAAACAGAAACCGAGACGAACCCAGCTGACGGAATCCGAACCGGAGCTCCCACGCGCTGGTGCGTGACGACAGACAGATTCCTTCAGGTGGCGTGTGAGCCTCACACGCGACGGTTTCCGGCGACCCACGAAGACAAATGGCGTCGGACGATGGCGGCCCTTCTCTTGAGGTGGGTGGTGTCGACAAACGGCCATTCGAACTCGATGACCAAAACACCAAACCCTAACTTTATGAAAATGTAGCCGTCAAATGAAAATTCTAAGCCCTAGAGGGCTATGATACCATGTACTCTATAATTTGGAGAATAATCTAATTGTATTATTCATTAGACTTAAGATACATATATATACATATATATATATATATATATAGGCGAGATGGAAACCCTAATCTAGAGAATGTGAAATTACAATAAAGGACACTATACGGTAAATACATATTATAACACCTTTCATTTGGTTAAGTGTGTGGTGGGTGATTGGGATGGTACCTTTTTTTGGGAGGATGAGTGGTTGGGGGATCTCGCCCCTTTTATAGTAGGTTCCCTAATCTCTATCATTTATCTACTATGAAAATCATTGCATTGCTAATGTCTTGGTTCATTCTAGGAGTTCTTGCTCTCCTTCTTTTGGGTTTCGTCATTCTTTGTCCAATAGAGAAACGATGGAGGTTATGACTATCCTTTGTTTATTAGGTGTGTTTGAGCTTAGACAGGGGAGAAGGGACTTGCATATTTTGAGTCTTTATCCCTCTTAGGGCTTTTCTTTTAGTTCTTTCTTCCATCATTTATTAGACTCTTCCCCTTCTAGGGAGTCTGTTTTCCCGGTTTTTTGGAAGGTCTTTGTCTGGCAAGTTATTCATGGAAGAATCAATACTTTGGATATGCTGTCGAGGAAGTTGCCGTCTTTGGTTGGGTCGTTCTGTTGTATCCTTTGTTGGAGGACAGAGGAAGATCTAGATCATTTATTATGGAATTGTTGGTTATGCTTAGTCGATGTGGAACTATTTTTTCATAGGGACATTTAGATAGCTCAACACAAAGCTTTTAGTAATGTGATCGAGGAGTTCCTCCTCTCTTAGCCCTTTTGAGAGAAAGAGAGTTTTTTTTTCTTTTTTTAAAATGAAAAGGGAGTTTGTGCTTTGTTATGGGGTATCTGGGGTGAGAGAAATAACATAGTTTTTAGGGGGGGATTGAGAGAGAGCCTTTGGATGTTTGGTCCATTATGAGGTTCTCTGACAAACTCCTTTTGTAATTTTTGACTTGGTTTTTTTTTTTTTTTTATATCCGTGAGTGTCTGGGCCAGCTTACACACACCTCGACTAATTTTTTACTTGGTTTTATTTTGCTTGATTGGAGCCCCTTTCTCTAGGATGCTTCTTTTTGTGGGCTTGATTTTTTTGTATGCCCTTGTATTCTTTTATTATTTTTTTTCTTAATGAAAGTTGGTATTTTATTTAAAAGAAAAAAAGGGAAAAAAAGGTTGTTTAAGTAGCATGACCTTAAAACTAACCTAGAATATGGGGAGTTTCAACGTTGTTAAGCTCTCTCTCTCTCTCTCTCTCTCTCTCGCTCTCTCTCTCTCTCTCTCTCTCTCTCAAAGGTTCAGTGCCTATTATCATTCAACATGTTGCTCTTGCTTCTGCCATTGAAATGCAACTATGATTCCTTCTCCCTTCCGTGCAAATGACAAAAAGATTAACTGAAAGTTTTATTTTCAATTGACATTGAATATGTAACAGATAAATCAATACCTGAGTTTGTATTACCGCACTGGTGTTCTTCCCAGCACACTTTTTCAAGGAAAGGCTGCTGAGGTATGAATCTTCTTTCCATTGAGCTATGTGACCATGGTAATTTGTGTGTGCGCTTGCCCTCCTTTTAGTTCTTCCTTGATGCTTGGACTTCTAAAATGAGTTTCTTTTCTTTAGATTATTCACTCATCATTTCACCTTGCCTGCTTAAGTCCTTGAATGATACCACACTGCTATCTTTAATACATGGTGTAACATTGCATGTGGCAGGGCAGAATTGGGGTCAAGCTGATAGTTTCAGATACGATTGCTGCTTGATATGCGTCGATGCTTCTGTTCCACAACTTGACGGACATGTTGATTCTAGAGTTGATAGCATGATGGCTGCTGGATTACTTGATGAGGTTTGTGACATTTACAATCCAAATGCAAACTATACTAGAGGATTGTGTCAAGCTATTGGTGTTAGGGAGTTTGAAGAGTTTTTGAGATATTATGTACCTAAAGGTGGATGCGAAAAAGATAGTGCTGGGCTTAGCCCAAAAAATTTGATGGAGTGCAATGAGATTTTTAAAGGGAATATGACGTCAATCCTAAATTCACCAAGTGATGCCCAACCCTGTTTGCTACTGAAAGAAGCCGTAGATAATGTAAAGTTGAATACTAGAAGACTGGTTCGTCGTCAGGTATGTGGATATCTATCTATGAACTTGTTTTCCTGA

mRNA sequence

TATAAATAGTCTAAAATTGACTCGTCGGAGAAGAGATGGGCGTCGTTGACGGTCTGCAAATCCGAACATGGGAAGAGAAAAGCCGAAGATGGTGGTGATTATGGGGCCGACTGGTTCTGGGAAGTCCCGTCTCGCTATCGATTTGGCTTCTCACTTCCCCGTCGAGATCATCAACGCCGACTCTATGCAAGTTTATCGAGGATTGGACGTTCTCACAAACAAAGTTCCCCCCGAAGACCAAAATGGAGTGCCGCATCATCTTTTGGGTACAGTGAGTCCGGACGTTGAATTCACGGCTAAACACTTCCGAGATTCTGCCGTTCCTGTTATTGATGACATAATTTCTCGTGGATGCTTGCCAATGATAGTGGGTGGAACGAATTATTATATACAGGCTCTTGTTAGCCCGTTTCTTCTGGATGATTCTGCGGAAACTTTTGGTGGAAGTTGCTCCATTTATCCTGGAGATGAAGAGCTGGGTATTGAATTGGATGAGGAAAGAGATGGGCAGAATTGGGGTCAAGCTGATAGTTTCAGATACGATTGCTGCTTGATATGCGTCGATGCTTCTGTTCCACAACTTGACGGACATGTTGATTCTAGAGTTGATAGCATGATGGCTGCTGGATTACTTGATGAGGTTTGTGACATTTACAATCCAAATGCAAACTATACTAGAGGATTGTGTCAAGCTATTGGTGTTAGGGAGTTTGAAGAGTTTTTGAGATATTATGTACCTAAAGGTGGATGCGAAAAAGATAGTGCTGGGCTTAGCCCAAAAAATTTGATGGAGTGCAATGAGATTTTTAAAGGGAATATGACGTCAATCCTAAATTCACCAAGTGATGCCCAACCCTGTTTGCTACTGAAAGAAGCCGTAGATAATGTAAAGTTGAATACTAGAAGACTGGTTCGTCGTCAGGTATGTGGATATCTATCTATGAACTTGTTTTCCTGA

Coding sequence (CDS)

ATGGGAAGAGAAAAGCCGAAGATGGTGGTGATTATGGGGCCGACTGGTTCTGGGAAGTCCCGTCTCGCTATCGATTTGGCTTCTCACTTCCCCGTCGAGATCATCAACGCCGACTCTATGCAAGTTTATCGAGGATTGGACGTTCTCACAAACAAAGTTCCCCCCGAAGACCAAAATGGAGTGCCGCATCATCTTTTGGGTACAGTGAGTCCGGACGTTGAATTCACGGCTAAACACTTCCGAGATTCTGCCGTTCCTGTTATTGATGACATAATTTCTCGTGGATGCTTGCCAATGATAGTGGGTGGAACGAATTATTATATACAGGCTCTTGTTAGCCCGTTTCTTCTGGATGATTCTGCGGAAACTTTTGGTGGAAGTTGCTCCATTTATCCTGGAGATGAAGAGCTGGGTATTGAATTGGATGAGGAAAGAGATGGGCAGAATTGGGGTCAAGCTGATAGTTTCAGATACGATTGCTGCTTGATATGCGTCGATGCTTCTGTTCCACAACTTGACGGACATGTTGATTCTAGAGTTGATAGCATGATGGCTGCTGGATTACTTGATGAGGTTTGTGACATTTACAATCCAAATGCAAACTATACTAGAGGATTGTGTCAAGCTATTGGTGTTAGGGAGTTTGAAGAGTTTTTGAGATATTATGTACCTAAAGGTGGATGCGAAAAAGATAGTGCTGGGCTTAGCCCAAAAAATTTGATGGAGTGCAATGAGATTTTTAAAGGGAATATGACGTCAATCCTAAATTCACCAAGTGATGCCCAACCCTGTTTGCTACTGAAAGAAGCCGTAGATAATGTAAAGTTGAATACTAGAAGACTGGTTCGTCGTCAGGTATGTGGATATCTATCTATGAACTTGTTTTCCTGA

Protein sequence

MGREKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNGVPHHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDSAETFGGSCSIYPGDEELGIELDEERDGQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLDEVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGNMTSILNSPSDAQPCLLLKEAVDNVKLNTRRLVRRQVCGYLSMNLFS
Homology
BLAST of Lsi03G000980 vs. ExPASy Swiss-Prot
Match: Q9ZUX7 (tRNA dimethylallyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=IPT2 PE=1 SV=2)

HSP 1 Score: 303.1 bits (775), Expect = 3.4e-81
Identity = 163/335 (48.66%), Postives = 217/335 (64.78%), Query Frame = 0

Query: 3   REKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNGVP 62
           ++K K+VVIMGPTGSGKS+LA+DLASHFPVEIINAD+MQ+Y GLDVLTNKV  ++Q GVP
Sbjct: 17  KKKAKVVVIMGPTGSGKSKLAVDLASHFPVEIINADAMQIYSGLDVLTNKVTVDEQKGVP 76

Query: 63  HHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDSAE 122
           HHLLGTVS D+EFTA+ FRD  VP+I++I+SR  +P++VGGT+YYIQA+VS FLLDD+AE
Sbjct: 77  HHLLGTVSSDMEFTARDFRDFTVPLIEEIVSRNHIPVLVGGTHYYIQAVVSKFLLDDAAE 136

Query: 123 TFGGSC--------------SIYPGDE-----ELGIELD--------------------- 182
                C              S++  D+     EL  ELD                     
Sbjct: 137 DTEECCADVASVVDQDMVVESVFGRDDLSHGYELLKELDPVAANRIHPNNHRKINQYLSL 196

Query: 183 ------------EERDGQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLD 242
                       + +  +NWG  ++ R+D CLIC+DA    LD +V+ RVD+M+ AGLLD
Sbjct: 197 HASRGVLPSKLYQGKTAENWGCINASRFDYCLICMDAETAVLDRYVEQRVDAMVDAGLLD 256

Query: 243 EVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGN 286
           EV DIY P A+YTRGL Q+IGVREFE+FL+ ++       ++      +L   +++ K N
Sbjct: 257 EVYDIYKPGADYTRGLRQSIGVREFEDFLKIHL------SETCAGHLTSLSNDDKVMKEN 316

BLAST of Lsi03G000980 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-44
Identity = 97/220 (44.09%), Postives = 133/220 (60.45%), Query Frame = 0

Query: 3   REKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNGVP 62
           R K K+V +MG TG+GKSRLAIDLA+ FP EI+N+D +QVY+GLD++TNKV PE+  GVP
Sbjct: 30  RRKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVP 89

Query: 63  HHLLGTVSPDVE-FTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDSA 122
           HHLLGTV    E FTA+ F+  A+  ++ I+ R  +P+I GG+N YI+ALV+        
Sbjct: 90  HHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVN-------- 149

Query: 123 ETFGGSCSIYPGDEELGIELDEERDGQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVD 182
                 C  +                         RY+CC + VD S P L   V  RVD
Sbjct: 150 -----DCVDF-----------------------RLRYNCCFLWVDVSRPVLHSFVSERVD 209

Query: 183 SMMAAGLLDEVCDIYNP-NANYTRGLCQAIGVREFEEFLR 221
            M+  GL+DEV  I++P +++Y+ G+ +AIGV E +EFLR
Sbjct: 210 KMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELDEFLR 213

BLAST of Lsi03G000980 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 8.1e-43
Identity = 89/217 (41.01%), Postives = 133/217 (61.29%), Query Frame = 0

Query: 5   KPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNGVPHH 64
           K K+VVIMG TG+GKSRL++D+A+ F  EIIN+D +QV++GLD++TNK+  E+  GVPHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 65  LLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDSAETF 124
           LLG + P+ + TA ++   A   I+ +++RG LP+IVGG+N Y++ALV     DD    F
Sbjct: 100 LLGVLPPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALV-----DDKENKF 159

Query: 125 GGSCSIYPGDEELGIELDEERDGQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMM 184
                                           RYDCC + VD ++P L G V  RVD M+
Sbjct: 160 RS------------------------------RYDCCFLWVDVALPVLHGFVSERVDKMV 219

Query: 185 AAGLLDEVCDIYN-PNANYTRGLCQAIGVREFEEFLR 221
            +G+++EV + ++  N++Y+RG+ +AIG  EF+ F R
Sbjct: 220 ESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFR 221

BLAST of Lsi03G000980 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 175.3 bits (443), Expect = 1.1e-42
Identity = 97/220 (44.09%), Postives = 127/220 (57.73%), Query Frame = 0

Query: 5   KPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNGVPHH 64
           K K++ +MG TGSGKSRLAIDLA+ F  EIIN+D +Q+Y+GLDVLTNKV P++  GVPHH
Sbjct: 33  KEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHH 92

Query: 65  LLGTVSPDV-EFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALV---SPFLLDDS 124
           LLG    +    TA  +   A   I  + +   LP++ GG+N YI+ALV   S FLL++ 
Sbjct: 93  LLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNN- 152

Query: 125 AETFGGSCSIYPGDEELGIELDEERDGQNWGQADSFRYDCCLICVDASVPQLDGHVDSRV 184
                                                YDCC I VD S+P L+  V  RV
Sbjct: 153 -------------------------------------YDCCFIWVDVSLPVLNSFVSKRV 212

Query: 185 DSMMAAGLLDEVCDIYNPNANYTRGLCQAIGVREFEEFLR 221
           D MM AGLL+EV +++NP ANY+ G+ +AIGV E  E+LR
Sbjct: 213 DRMMEAGLLEEVREVFNPKANYSVGIRRAIGVPELHEYLR 214

BLAST of Lsi03G000980 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.2e-41
Identity = 98/238 (41.18%), Postives = 137/238 (57.56%), Query Frame = 0

Query: 5   KPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNGVPHH 64
           K K++V+MG TG+GKSRL+IDLA+HFP+E+IN+D MQVY+GLD+ TNK+   D+ GVPHH
Sbjct: 29  KEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPHH 88

Query: 65  LLGTVSP-DVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDSAET 124
           LLG V P   E T   FR  A   + +I  R  LP++VGG+N +I A     LL D  ++
Sbjct: 89  LLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHA-----LLVDRFDS 148

Query: 125 FGGSCSIYPGDEELGIELDEERDGQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSM 184
            G      PG  E         +G +   +   RYDCC + VD SV  L  ++  RVD M
Sbjct: 149 SG------PGVFE---------EGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDM 208

Query: 185 MAAGLLDEVCDIYNP-------NANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAG 235
           +  G+ DE+ + Y+P       ++    GL +AIGV EF+ +   + P G  E +  G
Sbjct: 209 LELGMFDELAEFYSPEDEDHDEDSATRTGLRKAIGVPEFDRYFEKFRP-GDVEGEDPG 245

BLAST of Lsi03G000980 vs. ExPASy TrEMBL
Match: A0A0A0LVP2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G152000 PE=3 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 6.9e-138
Identity = 253/335 (75.52%), Postives = 264/335 (78.81%), Query Frame = 0

Query: 1   MGREKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 60
           MG EKPK+VVIMGPTGSGKSRLAIDLAS+FPVEIINADSMQVYRGLDVLTNKVPPEDQNG
Sbjct: 17  MGGEKPKVVVIMGPTGSGKSRLAIDLASYFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 76

Query: 61  VPHHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS 120
           VPHHLLGTVSPDVEFTAK FR+SAV VID IISRGCLPMIVGGTNYYIQALVSPFLLDDS
Sbjct: 77  VPHHLLGTVSPDVEFTAKDFRNSAVHVIDGIISRGCLPMIVGGTNYYIQALVSPFLLDDS 136

Query: 121 AETFGGSCSIYPGDEELGIELDEERDG--------------------------------- 180
           AE  GG CSIYPGDE LG++LDE RDG                                 
Sbjct: 137 AENVGGGCSIYPGDEGLGVDLDECRDGLKYDHNHLKEIDPVSANRIHPNNHRKINQYLSL 196

Query: 181 -----------------QNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLD 240
                            +NWGQADSF+YDCCLICVDASVPQLD HVDSRVD MMAAGLLD
Sbjct: 197 YYRTGVLPSTLFQGKAAENWGQADSFKYDCCLICVDASVPQLDEHVDSRVDIMMAAGLLD 256

Query: 241 EVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGN 286
           EV DIYNPNANYTRGLCQAIGVREFEEF R Y+P+GGC+K+SAGLSPKNLMECNEI K N
Sbjct: 257 EVYDIYNPNANYTRGLCQAIGVREFEEFFRCYIPEGGCKKESAGLSPKNLMECNEILKEN 316

BLAST of Lsi03G000980 vs. ExPASy TrEMBL
Match: A0A5A7TFW5 (tRNA dimethylallyltransferase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold125G001280 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.5e-137
Identity = 253/336 (75.30%), Postives = 266/336 (79.17%), Query Frame = 0

Query: 1   MGREKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 60
           MGREKPKMVVIMGPTGSGKSRLAIDLAS+FPVEIINADSMQVYRGLDVLTNKVPPEDQNG
Sbjct: 65  MGREKPKMVVIMGPTGSGKSRLAIDLASYFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 124

Query: 61  VPHHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS 120
           VPHHLLGTVSPDVEFTAK FR+SAV VID IISRGCLPMIVGGTNYYIQALVS FLLDDS
Sbjct: 125 VPHHLLGTVSPDVEFTAKDFRNSAVHVIDGIISRGCLPMIVGGTNYYIQALVSLFLLDDS 184

Query: 121 AETFGGSCSIYPGDEELGIELDEERDG--------------------------------- 180
           AE  GG CSIYPGDEELG++LDE RDG                                 
Sbjct: 185 AENVGGGCSIYPGDEELGVDLDEGRDGLKYDHNHLKEIDPVSANRIHPNNHRKINQYLSL 244

Query: 181 -----------------QNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLD 240
                            +NWGQADSFRYDCCLICVDASVPQLD HVDSRVD MMAAGLLD
Sbjct: 245 YYRTGVLPSTLFQGKAAENWGQADSFRYDCCLICVDASVPQLDEHVDSRVDIMMAAGLLD 304

Query: 241 EVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGN 287
           EV DIY+PNANYTRGL QAIGVREFEEFLRYY+P+GG +K+SAG SPKNLMECNEI K +
Sbjct: 305 EVYDIYDPNANYTRGLRQAIGVREFEEFLRYYIPEGGFKKESAGFSPKNLMECNEILKES 364

BLAST of Lsi03G000980 vs. ExPASy TrEMBL
Match: A0A1S3CGY2 (tRNA dimethylallyltransferase 2 OS=Cucumis melo OX=3656 GN=LOC103500335 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.0e-136
Identity = 253/335 (75.52%), Postives = 265/335 (79.10%), Query Frame = 0

Query: 1   MGREKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 60
           MGREKPKMVVIMGPTGSGKSRLAIDLAS+FPVEIINADSMQVYRGLDVLTNKVPPEDQNG
Sbjct: 17  MGREKPKMVVIMGPTGSGKSRLAIDLASYFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 76

Query: 61  VPHHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS 120
           VPHHLLGTVSPDVEFTAK FR+SAV VID IISRGCLPMIVGGTNYYIQALVS FLLDDS
Sbjct: 77  VPHHLLGTVSPDVEFTAKDFRNSAVHVIDGIISRGCLPMIVGGTNYYIQALVSLFLLDDS 136

Query: 121 AETFGGSCSIYPGDEELGIELDEERDG--------------------------------- 180
           AE  GG CSIYPGDEELG++LDE RDG                                 
Sbjct: 137 AENVGGGCSIYPGDEELGVDLDEGRDGLKYDHNHLKEIDPVSANRIHPNNHRKINQYLSL 196

Query: 181 -----------------QNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLD 240
                            +NWGQADSFRYDCCLICVDASVPQLD HVDSRVD MMAAGLLD
Sbjct: 197 YYRTGVLPSTLFQGKAAENWGQADSFRYDCCLICVDASVPQLDEHVDSRVDIMMAAGLLD 256

Query: 241 EVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGN 286
           EV DIY+PNANYTRGL QAIGVREFEEFLRYY+P+GG +K+SAG SPKNLMECNEI K +
Sbjct: 257 EVYDIYDPNANYTRGLRQAIGVREFEEFLRYYIPEGGFKKESAGFSPKNLMECNEILKES 316

BLAST of Lsi03G000980 vs. ExPASy TrEMBL
Match: A0A6J1HWL0 (tRNA dimethylallyltransferase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468528 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.5e-132
Identity = 243/335 (72.54%), Postives = 260/335 (77.61%), Query Frame = 0

Query: 1   MGREKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 60
           MGREKPK+VVIMGPTGSGKSRLAIDLAS+FPVEIINADSMQVYRGLDVLTNKVPP+DQNG
Sbjct: 17  MGREKPKVVVIMGPTGSGKSRLAIDLASYFPVEIINADSMQVYRGLDVLTNKVPPQDQNG 76

Query: 61  VPHHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS 120
           VPHHLLGTVSPDVEFTAK FRDSA+PVIDDI+SRGCLPMIVGGTNYYIQAL SP LLDDS
Sbjct: 77  VPHHLLGTVSPDVEFTAKDFRDSAIPVIDDILSRGCLPMIVGGTNYYIQALFSPLLLDDS 136

Query: 121 AETFGGSCSIYPGDEELGIELDEERD---------------------------------- 180
           A+  GGSCS Y GDEE+ IELDE RD                                  
Sbjct: 137 AKNIGGSCSSYLGDEEMDIELDEGRDSLKYDHSYLKEIDPVSAGRLHPNNHRKINQYLSL 196

Query: 181 ----------------GQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLD 240
                            ++WGQADSFRY+CCLICVDAS+PQ+DGHVDSRVDSM+AAGLLD
Sbjct: 197 YYRTGVLPSTLFQGKAAESWGQADSFRYECCLICVDASIPQIDGHVDSRVDSMLAAGLLD 256

Query: 241 EVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGN 286
           EV DIYNPNANYTRGL QAIGVREFEEFLRYYVP G C++DS  LSPKN MECNEI K N
Sbjct: 257 EVYDIYNPNANYTRGLWQAIGVREFEEFLRYYVPNGRCDEDSNELSPKNSMECNEILKEN 316

BLAST of Lsi03G000980 vs. ExPASy TrEMBL
Match: A0A6J1FCD1 (tRNA dimethylallyltransferase 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444115 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.5e-132
Identity = 243/335 (72.54%), Postives = 259/335 (77.31%), Query Frame = 0

Query: 1   MGREKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 60
           MGREKPK+VVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPP+DQNG
Sbjct: 17  MGREKPKVVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPQDQNG 76

Query: 61  VPHHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS 120
           VPHHLLGTVSPDVEFTAK FRDSA+PVIDDI+SRGCLPMIVGGTNYYIQAL SP LLDDS
Sbjct: 77  VPHHLLGTVSPDVEFTAKDFRDSAIPVIDDILSRGCLPMIVGGTNYYIQALFSPLLLDDS 136

Query: 121 AETFGGSCSIYPGDEELGIELDEERD---------------------------------- 180
           A+  GGSCS Y GDEE+ IELDE RD                                  
Sbjct: 137 AKNIGGSCSSYLGDEEMDIELDEGRDSLKYDHSYLKEIDPVSAGRLHPNNHRKINQYLSL 196

Query: 181 ----------------GQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLD 240
                            ++WGQADSFRYDCCLICVDAS+PQ+DGHVDSRVDSM+AAGLLD
Sbjct: 197 YYRTGVLPSTLFQGKAAESWGQADSFRYDCCLICVDASIPQIDGHVDSRVDSMLAAGLLD 256

Query: 241 EVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGN 286
           EV +IYNPNANYTRGL QAIGVREFEEFLRYYVP G C++DS  LSPKN MEC EI K N
Sbjct: 257 EVYEIYNPNANYTRGLWQAIGVREFEEFLRYYVPNGRCDEDSNELSPKNSMECTEILKEN 316

BLAST of Lsi03G000980 vs. NCBI nr
Match: XP_038895131.1 (tRNA dimethylallyltransferase 2 isoform X2 [Benincasa hispida])

HSP 1 Score: 501.5 bits (1290), Expect = 4.9e-138
Identity = 256/335 (76.42%), Postives = 263/335 (78.51%), Query Frame = 0

Query: 1   MGREKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 60
           MGRE PK+VVIMGPTGSGKSRLAIDLAS FPVEIINADSMQVYRGLDVLTNKVPPEDQNG
Sbjct: 17  MGREMPKVVVIMGPTGSGKSRLAIDLASLFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 76

Query: 61  VPHHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS 120
           VPHHLLGTVSPDVEFTAK FRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS
Sbjct: 77  VPHHLLGTVSPDVEFTAKDFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS 136

Query: 121 AETFGGSCSIYPGDEELGIELDEERD---------------------------------- 180
            E   GSCSIY GDEELGIELDE RD                                  
Sbjct: 137 TENISGSCSIYHGDEELGIELDEGRDDLKYDHNHLKKIDPVSANRIHPNNHRKINQYLSL 196

Query: 181 ----------------GQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLD 240
                            +NWGQAD+FRY+CCLICVDASVPQLDGHV+SRVDSMM AGLLD
Sbjct: 197 YYRTGVLPSILFQGKAAENWGQADNFRYNCCLICVDASVPQLDGHVNSRVDSMMTAGLLD 256

Query: 241 EVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGN 286
           EV DIYNPNANYTRGL QAIGVREFEEFLRY+VPKG CE DSAG SPKNLMECNEI K N
Sbjct: 257 EVYDIYNPNANYTRGLRQAIGVREFEEFLRYHVPKGRCEDDSAGFSPKNLMECNEILKEN 316

BLAST of Lsi03G000980 vs. NCBI nr
Match: XP_038895129.1 (tRNA dimethylallyltransferase 2 isoform X1 [Benincasa hispida] >XP_038895130.1 tRNA dimethylallyltransferase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 501.5 bits (1290), Expect = 4.9e-138
Identity = 256/335 (76.42%), Postives = 263/335 (78.51%), Query Frame = 0

Query: 1   MGREKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 60
           MGRE PK+VVIMGPTGSGKSRLAIDLAS FPVEIINADSMQVYRGLDVLTNKVPPEDQNG
Sbjct: 17  MGREMPKVVVIMGPTGSGKSRLAIDLASLFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 76

Query: 61  VPHHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS 120
           VPHHLLGTVSPDVEFTAK FRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS
Sbjct: 77  VPHHLLGTVSPDVEFTAKDFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS 136

Query: 121 AETFGGSCSIYPGDEELGIELDEERD---------------------------------- 180
            E   GSCSIY GDEELGIELDE RD                                  
Sbjct: 137 TENISGSCSIYHGDEELGIELDEGRDDLKYDHNHLKKIDPVSANRIHPNNHRKINQYLSL 196

Query: 181 ----------------GQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLD 240
                            +NWGQAD+FRY+CCLICVDASVPQLDGHV+SRVDSMM AGLLD
Sbjct: 197 YYRTGVLPSILFQGKAAENWGQADNFRYNCCLICVDASVPQLDGHVNSRVDSMMTAGLLD 256

Query: 241 EVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGN 286
           EV DIYNPNANYTRGL QAIGVREFEEFLRY+VPKG CE DSAG SPKNLMECNEI K N
Sbjct: 257 EVYDIYNPNANYTRGLRQAIGVREFEEFLRYHVPKGRCEDDSAGFSPKNLMECNEILKEN 316

BLAST of Lsi03G000980 vs. NCBI nr
Match: XP_004139473.1 (tRNA dimethylallyltransferase 2 [Cucumis sativus] >KGN64902.1 hypothetical protein Csa_022787 [Cucumis sativus])

HSP 1 Score: 500.0 bits (1286), Expect = 1.4e-137
Identity = 253/335 (75.52%), Postives = 264/335 (78.81%), Query Frame = 0

Query: 1   MGREKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 60
           MG EKPK+VVIMGPTGSGKSRLAIDLAS+FPVEIINADSMQVYRGLDVLTNKVPPEDQNG
Sbjct: 17  MGGEKPKVVVIMGPTGSGKSRLAIDLASYFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 76

Query: 61  VPHHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS 120
           VPHHLLGTVSPDVEFTAK FR+SAV VID IISRGCLPMIVGGTNYYIQALVSPFLLDDS
Sbjct: 77  VPHHLLGTVSPDVEFTAKDFRNSAVHVIDGIISRGCLPMIVGGTNYYIQALVSPFLLDDS 136

Query: 121 AETFGGSCSIYPGDEELGIELDEERDG--------------------------------- 180
           AE  GG CSIYPGDE LG++LDE RDG                                 
Sbjct: 137 AENVGGGCSIYPGDEGLGVDLDECRDGLKYDHNHLKEIDPVSANRIHPNNHRKINQYLSL 196

Query: 181 -----------------QNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLD 240
                            +NWGQADSF+YDCCLICVDASVPQLD HVDSRVD MMAAGLLD
Sbjct: 197 YYRTGVLPSTLFQGKAAENWGQADSFKYDCCLICVDASVPQLDEHVDSRVDIMMAAGLLD 256

Query: 241 EVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGN 286
           EV DIYNPNANYTRGLCQAIGVREFEEF R Y+P+GGC+K+SAGLSPKNLMECNEI K N
Sbjct: 257 EVYDIYNPNANYTRGLCQAIGVREFEEFFRCYIPEGGCKKESAGLSPKNLMECNEILKEN 316

BLAST of Lsi03G000980 vs. NCBI nr
Match: KAA0040936.1 (tRNA dimethylallyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 497.3 bits (1279), Expect = 9.3e-137
Identity = 253/336 (75.30%), Postives = 266/336 (79.17%), Query Frame = 0

Query: 1   MGREKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 60
           MGREKPKMVVIMGPTGSGKSRLAIDLAS+FPVEIINADSMQVYRGLDVLTNKVPPEDQNG
Sbjct: 65  MGREKPKMVVIMGPTGSGKSRLAIDLASYFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 124

Query: 61  VPHHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS 120
           VPHHLLGTVSPDVEFTAK FR+SAV VID IISRGCLPMIVGGTNYYIQALVS FLLDDS
Sbjct: 125 VPHHLLGTVSPDVEFTAKDFRNSAVHVIDGIISRGCLPMIVGGTNYYIQALVSLFLLDDS 184

Query: 121 AETFGGSCSIYPGDEELGIELDEERDG--------------------------------- 180
           AE  GG CSIYPGDEELG++LDE RDG                                 
Sbjct: 185 AENVGGGCSIYPGDEELGVDLDEGRDGLKYDHNHLKEIDPVSANRIHPNNHRKINQYLSL 244

Query: 181 -----------------QNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLD 240
                            +NWGQADSFRYDCCLICVDASVPQLD HVDSRVD MMAAGLLD
Sbjct: 245 YYRTGVLPSTLFQGKAAENWGQADSFRYDCCLICVDASVPQLDEHVDSRVDIMMAAGLLD 304

Query: 241 EVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGN 287
           EV DIY+PNANYTRGL QAIGVREFEEFLRYY+P+GG +K+SAG SPKNLMECNEI K +
Sbjct: 305 EVYDIYDPNANYTRGLRQAIGVREFEEFLRYYIPEGGFKKESAGFSPKNLMECNEILKES 364

BLAST of Lsi03G000980 vs. NCBI nr
Match: XP_008461831.1 (PREDICTED: tRNA dimethylallyltransferase 2 [Cucumis melo])

HSP 1 Score: 496.1 bits (1276), Expect = 2.1e-136
Identity = 253/335 (75.52%), Postives = 265/335 (79.10%), Query Frame = 0

Query: 1   MGREKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 60
           MGREKPKMVVIMGPTGSGKSRLAIDLAS+FPVEIINADSMQVYRGLDVLTNKVPPEDQNG
Sbjct: 17  MGREKPKMVVIMGPTGSGKSRLAIDLASYFPVEIINADSMQVYRGLDVLTNKVPPEDQNG 76

Query: 61  VPHHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDS 120
           VPHHLLGTVSPDVEFTAK FR+SAV VID IISRGCLPMIVGGTNYYIQALVS FLLDDS
Sbjct: 77  VPHHLLGTVSPDVEFTAKDFRNSAVHVIDGIISRGCLPMIVGGTNYYIQALVSLFLLDDS 136

Query: 121 AETFGGSCSIYPGDEELGIELDEERDG--------------------------------- 180
           AE  GG CSIYPGDEELG++LDE RDG                                 
Sbjct: 137 AENVGGGCSIYPGDEELGVDLDEGRDGLKYDHNHLKEIDPVSANRIHPNNHRKINQYLSL 196

Query: 181 -----------------QNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLD 240
                            +NWGQADSFRYDCCLICVDASVPQLD HVDSRVD MMAAGLLD
Sbjct: 197 YYRTGVLPSTLFQGKAAENWGQADSFRYDCCLICVDASVPQLDEHVDSRVDIMMAAGLLD 256

Query: 241 EVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGN 286
           EV DIY+PNANYTRGL QAIGVREFEEFLRYY+P+GG +K+SAG SPKNLMECNEI K +
Sbjct: 257 EVYDIYDPNANYTRGLRQAIGVREFEEFLRYYIPEGGFKKESAGFSPKNLMECNEILKES 316

BLAST of Lsi03G000980 vs. TAIR 10
Match: AT2G27760.1 (tRNAisopentenyltransferase 2 )

HSP 1 Score: 303.1 bits (775), Expect = 2.4e-82
Identity = 163/335 (48.66%), Postives = 217/335 (64.78%), Query Frame = 0

Query: 3   REKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNGVP 62
           ++K K+VVIMGPTGSGKS+LA+DLASHFPVEIINAD+MQ+Y GLDVLTNKV  ++Q GVP
Sbjct: 17  KKKAKVVVIMGPTGSGKSKLAVDLASHFPVEIINADAMQIYSGLDVLTNKVTVDEQKGVP 76

Query: 63  HHLLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDSAE 122
           HHLLGTVS D+EFTA+ FRD  VP+I++I+SR  +P++VGGT+YYIQA+VS FLLDD+AE
Sbjct: 77  HHLLGTVSSDMEFTARDFRDFTVPLIEEIVSRNHIPVLVGGTHYYIQAVVSKFLLDDAAE 136

Query: 123 TFGGSC--------------SIYPGDE-----ELGIELD--------------------- 182
                C              S++  D+     EL  ELD                     
Sbjct: 137 DTEECCADVASVVDQDMVVESVFGRDDLSHGYELLKELDPVAANRIHPNNHRKINQYLSL 196

Query: 183 ------------EERDGQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMMAAGLLD 242
                       + +  +NWG  ++ R+D CLIC+DA    LD +V+ RVD+M+ AGLLD
Sbjct: 197 HASRGVLPSKLYQGKTAENWGCINASRFDYCLICMDAETAVLDRYVEQRVDAMVDAGLLD 256

Query: 243 EVCDIYNPNANYTRGLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKNLMECNEIFKGN 286
           EV DIY P A+YTRGL Q+IGVREFE+FL+ ++       ++      +L   +++ K N
Sbjct: 257 EVYDIYKPGADYTRGLRQSIGVREFEDFLKIHL------SETCAGHLTSLSNDDKVMKEN 316

BLAST of Lsi03G000980 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 181.0 bits (458), Expect = 1.4e-45
Identity = 97/220 (44.09%), Postives = 133/220 (60.45%), Query Frame = 0

Query: 3   REKPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNGVP 62
           R K K+V +MG TG+GKSRLAIDLA+ FP EI+N+D +QVY+GLD++TNKV PE+  GVP
Sbjct: 30  RRKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVP 89

Query: 63  HHLLGTVSPDVE-FTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDSA 122
           HHLLGTV    E FTA+ F+  A+  ++ I+ R  +P+I GG+N YI+ALV+        
Sbjct: 90  HHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVN-------- 149

Query: 123 ETFGGSCSIYPGDEELGIELDEERDGQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVD 182
                 C  +                         RY+CC + VD S P L   V  RVD
Sbjct: 150 -----DCVDF-----------------------RLRYNCCFLWVDVSRPVLHSFVSERVD 209

Query: 183 SMMAAGLLDEVCDIYNP-NANYTRGLCQAIGVREFEEFLR 221
            M+  GL+DEV  I++P +++Y+ G+ +AIGV E +EFLR
Sbjct: 210 KMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELDEFLR 213

BLAST of Lsi03G000980 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 175.6 bits (444), Expect = 5.8e-44
Identity = 89/217 (41.01%), Postives = 133/217 (61.29%), Query Frame = 0

Query: 5   KPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNGVPHH 64
           K K+VVIMG TG+GKSRL++D+A+ F  EIIN+D +QV++GLD++TNK+  E+  GVPHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 65  LLGTVSPDVEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDSAETF 124
           LLG + P+ + TA ++   A   I+ +++RG LP+IVGG+N Y++ALV     DD    F
Sbjct: 100 LLGVLPPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALV-----DDKENKF 159

Query: 125 GGSCSIYPGDEELGIELDEERDGQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDSMM 184
                                           RYDCC + VD ++P L G V  RVD M+
Sbjct: 160 RS------------------------------RYDCCFLWVDVALPVLHGFVSERVDKMV 219

Query: 185 AAGLLDEVCDIYN-PNANYTRGLCQAIGVREFEEFLR 221
            +G+++EV + ++  N++Y+RG+ +AIG  EF+ F R
Sbjct: 220 ESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFR 221

BLAST of Lsi03G000980 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 175.3 bits (443), Expect = 7.5e-44
Identity = 97/220 (44.09%), Postives = 127/220 (57.73%), Query Frame = 0

Query: 5   KPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNGVPHH 64
           K K++ +MG TGSGKSRLAIDLA+ F  EIIN+D +Q+Y+GLDVLTNKV P++  GVPHH
Sbjct: 33  KEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHH 92

Query: 65  LLGTVSPDV-EFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALV---SPFLLDDS 124
           LLG    +    TA  +   A   I  + +   LP++ GG+N YI+ALV   S FLL++ 
Sbjct: 93  LLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNN- 152

Query: 125 AETFGGSCSIYPGDEELGIELDEERDGQNWGQADSFRYDCCLICVDASVPQLDGHVDSRV 184
                                                YDCC I VD S+P L+  V  RV
Sbjct: 153 -------------------------------------YDCCFIWVDVSLPVLNSFVSKRV 212

Query: 185 DSMMAAGLLDEVCDIYNPNANYTRGLCQAIGVREFEEFLR 221
           D MM AGLL+EV +++NP ANY+ G+ +AIGV E  E+LR
Sbjct: 213 DRMMEAGLLEEVREVFNPKANYSVGIRRAIGVPELHEYLR 214

BLAST of Lsi03G000980 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 167.9 bits (424), Expect = 1.2e-41
Identity = 104/287 (36.24%), Postives = 157/287 (54.70%), Query Frame = 0

Query: 5   KPKMVVIMGPTGSGKSRLAIDLASHFPVEIINADSMQVYRGLDVLTNKVPPEDQNGVPHH 64
           K K+VVI+G TG+GKSRL++DLA+ FP EIIN+D +QVY GL++ TN++  +D+ GVPHH
Sbjct: 64  KDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHH 123

Query: 65  LLGTVSPD-VEFTAKHFRDSAVPVIDDIISRGCLPMIVGGTNYYIQALVSPFLLDDSAET 124
           LLG ++P+  E TA  FR +A  V+ +I SR  +P+I GG+N ++ AL++    D   + 
Sbjct: 124 LLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQ-RFDPKFDP 183

Query: 125 F-GGSCSIYPGDEELGIELDEERDGQNWGQADSFRYDCCLICVDASVPQLDGHVDSRVDS 184
           F  GSC I                      +   RY+CC I VD S   L  ++  RVD 
Sbjct: 184 FSSGSCLI----------------------SSDLRYECCFIWVDVSETVLYEYLLRRVDE 243

Query: 185 MMAAGLLDEVCDIYNP--NANYTR-GLCQAIGVREFEEFLRYYVPKGGCEKDSAGLSPKN 244
           MM +G+ +E+   Y+P  +   TR G+ +AIGV EF+ + + Y P+            K 
Sbjct: 244 MMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPE------------KK 299

Query: 245 LMECNEIFKGNMTSILNSPSDAQPCLLLKEAVDNVKLNTRRLVRRQV 287
           +++ + + K                    +AVD++K NT  L +RQV
Sbjct: 304 MIKWDALRKA----------------AYDKAVDDIKRNTWTLAKRQV 299

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZUX73.4e-8148.66tRNA dimethylallyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=IPT2 PE=1 SV=... [more]
Q94ID21.9e-4444.09Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q93WC98.1e-4341.01Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID11.1e-4244.09Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q5GHF71.2e-4141.18Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LVP26.9e-13875.52Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G152000 PE=3 SV=1[more]
A0A5A7TFW54.5e-13775.30tRNA dimethylallyltransferase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3CGY21.0e-13675.52tRNA dimethylallyltransferase 2 OS=Cucumis melo OX=3656 GN=LOC103500335 PE=3 SV=... [more]
A0A6J1HWL01.5e-13272.54tRNA dimethylallyltransferase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1FCD11.5e-13272.54tRNA dimethylallyltransferase 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_038895131.14.9e-13876.42tRNA dimethylallyltransferase 2 isoform X2 [Benincasa hispida][more]
XP_038895129.14.9e-13876.42tRNA dimethylallyltransferase 2 isoform X1 [Benincasa hispida] >XP_038895130.1 t... [more]
XP_004139473.11.4e-13775.52tRNA dimethylallyltransferase 2 [Cucumis sativus] >KGN64902.1 hypothetical prote... [more]
KAA0040936.19.3e-13775.30tRNA dimethylallyltransferase 2 [Cucumis melo var. makuwa][more]
XP_008461831.12.1e-13675.52PREDICTED: tRNA dimethylallyltransferase 2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT2G27760.12.4e-8248.66tRNAisopentenyltransferase 2 [more]
AT5G19040.11.4e-4544.09isopentenyltransferase 5 [more]
AT3G63110.15.8e-4441.01isopentenyltransferase 3 [more]
AT3G23630.17.5e-4444.09isopentenyltransferase 7 [more]
AT1G68460.11.2e-4136.24isopentenyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01715IPPTcoord: 153..286
e-value: 1.1E-12
score: 48.2
coord: 41..115
e-value: 8.1E-25
score: 87.9
NoneNo IPR availablePANTHERPTHR11088:SF25TRNA DIMETHYLALLYLTRANSFERASEcoord: 4..285
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 6..289
e-value: 2.8E-77
score: 261.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 8..114
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 4..285
IPR018022IPP transferaseHAMAPMF_00185IPP_transcoord: 8..296
score: 18.339371

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G000980.1Lsi03G000980.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0006400 tRNA modification
biological_process GO:0008033 tRNA processing
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0052381 tRNA dimethylallyltransferase activity