Lsi03G000710 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G000710
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionlysosomal Pro-X carboxypeptidase-like
Locationchr03: 1011752 .. 1019471 (-)
RNA-Seq ExpressionLsi03G000710
SyntenyLsi03G000710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATATGTAAAAAGAAACTGTAAAATTTAAAAACTCCAAAGCCATAGAAGCTTTTTAGATTTGAAATTGTTTTTTAGATATATTCTCATAAAATAAGGATGGTAGAGAATTCAAAATTTGAGTAAGAACTTCATTATTAGAAAGATGAAAGGAAAAGAAAGACAAAATTTTGGTAATAAGAAAAAGTGGAAATGATTGAAAATGGAACCAAAGTTCCACATTGGTTAGATTATGGAAAGATCACGAGTATATATATTATCATGAAATCGACAAACAAAGAAAATAGAAAGTATATAAATAGTAGAGAATTGACACATAGATTTACGTGGTTCGCTAACAGTGTGTTAGCTACATCCACAAGCAAAAGGAAAAAGTCCCTCAATAGAGTTAAATAGTTTAGATAAGCATTAAATATATAACATAAATACAAATACAACTTAAGTTTCAGGGGTGTTGTTGACCAAACAGCATTAAATATATAACATAAATACAAATACAACTTAAGTTTCAGGGGTGTTGTTGACCAGTCAATGGGACCCCATGCTTGGTCGTTCAAAGCGCATCTCCAATGGTATGAGATCGTTTGGTTTAAAACCAAAAGTAAATTCATGAGGTGCTGACCAGTTGAGACCCCATGCTTGGTCATTCAATGCGTGAGCACAACTATCTCCCATGGTATGAGACTTTTCGGAGGGTGAAACCAAAAGTAAAGTCACGAGATTTATGTCCAAAGTGGACAATATCATACCATATTATAAAGATAGATGAAAGCACATTGTCTCCAATAGAAAATTTAAGATCTTTTTGTACATCATAACTTTTGTGAAGCTATAAATACATATCCGCAGCTAAAGTTGATAAATGTGGGAAACAAATAACATGGACTACATTGTGAGCCAATCCATATTTCTTCCACTACTTCTTCTTTTCGTTTCTTCTTGTGTTTCTGGCCACATCCCACGCCTTGGAGTGCAAAGAAGGGCATTCCAAAATAAGCCCCAAGAACTAGACAAAACACCGGATGACTTAGCAACTTTCTATTACAAACAACCACTTGATCACTTCAATTATCATCCTCAAAGTTACGTTACGTTTGATCAAAGATATATTATTGATTTTAGGTACTGGAAAGGTATCGATCCCAAAACTCCCATCTTTGCTTTTCTCGGTGCTGAAAGTGATATCGACAATGACATACCTAGCGTTGGCTTTCCCCTCCATTTCGCCTCTCGATACAAGGCTATGTCGGTGTATTTAGAGGTATATACATGCTAAATAATATATCAAATAGATTGGCATATTAACAATCATTATTATCGTCGTCGTCGTCGTCAATATAAATAGAAGGAGGAAATTGAAATCAAAAAGTCTCTTTATCGTAAACATATACTATGTATCAGTTAAGCTATTCTCGCTTTGACACACACATATCATTTTTTTTAATTTGACAGGTCATTGCTATATATATATATATATATATATATACATATACATATACACGCGCTTTTAAGCTAGTGCTAACCATGTGTGGATAAACTATCACTAAACTAAAAAAAATTAAATGTTAAAAATATCATTTTGGCTCTTTTATTTAGGACCCGTTTGAATTGACTTTTTAAGTGTTTATAAACACTTTTCTTTTTGTATTTATAAACATTTATTTAAAGCACTTAGAAAGTCAATCCAGACAGGTCATTAGATTTTTTTTGTTATATTTTGGTCTTTATACTTTCAAATGTTTAATTTTAGTCATTATGTCGAGCCTAAATATATTTTTAATCTTATATGTATATAAAAGGATTAGTTTTTGTTTTTGCTTTTGACATGTATATTTTTAATCTTAACAGCATCGATTTTATGGGAAATCAGTACCATTTGGTTCAATAGAGAAGGCAATGAAAAATGCTAGCATTCGAGGATACTTTAATTCAGCTCAAGCCTTAGCAGATTATGCTGACGTGCTTTTGAACATCAAGCAAAGGTTTGCATATGAGACTTCTCCAATTATAGTTATTGGAGCCTCCTATGGTGGAAGTAAGTCACCCTATTTAATTCTCTCTTAATATAAATTTCCATTAATTTAACTAAATAATTCTTCCCATTATTAAACTCTCTATATATATTTCTAAAAGAAAAATTTTCACATAGAGAAAAAATGCAAAAAACACTAATAGACATTGATAGACTTCTATTATTTTTATCACTCATAAATCCGAATAGACTTCTATACATAGCGTTTATCACAACCATCTAAAGAAGTTCTAATAAGTCTTTAAATTTTTCAATTTTGTGTCTTACAAAGTCTACGCCTACATTTATGTAGTATGTTTATGTATTATTTAAGATACAATGACTTTTGTTTGATTAATTATATTACTTTATTTTAATTTACCATGGATGGACAGTGTTAGCTTCATGGTTCAGGCTAAAGTATCCTCATATCGCTCTTGGAGCCCTCGCTTCTTCAGCCCCAATCCTTTACTTTGACAACATCACACCTCAAGACGGATATTACTCCATCGTCTCCAAATCCTTCAAAGTACGTATGTTTCCTTGATCAATTAAAACCCTAAACTGGAACCAATTTCTTTTTCAAACAAACTTGATTCGCTTCAAAAATTTTATTAAGGACACATGACGATGATCATTATAACAACGAAACATTCCATTGATGAAATGAAATTAGGAAACAAGCAAAACATGTCACGACACCATACGCAGATCATGGGGTGAAATCGACCGAATCACTGAGAAAACTCCGGGAGGGCTTTCAATTCTAAGCAAGCGATTCAAAATTTGTGGGAAATTGAATATGAGTACGGATATCAAGAACCTTTTGGACAGTATGTATACATGGGCAGCTCAATACAACGATCCACATGATAAACCAGTGAGAACCATATGTGCAGCCATTGATGAAGAAGCCAAGAAAAAAAGTGATTTGATTCAGCAAGTGTTTGCAGGGCTTATTGCTGTCGTGGGGGAGAGATCTTGTTATGATGTCTATGAATTTGACGAGCCTGATGATCCCATTTATGATCTCTATGGTTGGCAGGTACTCCTTCCTTTTTAATCTTACATTTTTTTAAATTGATAGTTTTAAATGTAGTAAAATAAGTTAAAATATTTATAAATATAGCAAAATATCACTATCTATCCGTAATAGTCATTCATAAATACTCTCTATCGCTATCTATCATTGACATTTTGCTATATTTATAAATATTTCCTGCAATTTTATCATTTAAAACAATTACTCTTTTTAAAACTACATTTCTTTTTAGAAAATTTTCGTAAATAGAAAAAAAATGTCAAATTATTTACCGAAAATAGCAAAAAAAAAATACTGATGGACTTCTATAGCATCTATCAGTGATAGACTTCTATCATTTCTATCACTTATAGACTTTGATAAACTTCTATCAACGTCTATCACAACTATCTAAAAATTTTACTATTTTGTGTAAATAGTTTCTCTAATTTTTCTATTTTTAAATATTCCCCTTTCTTTTTTTATTATCTTTATTTTACTATCTTAAAATGTGGCTAACGAGAATAAATTATTATTTTTAAACTATTTTGTCCAAATCTTAATTTCGTAATTCTAAATGTCATTAAATTATTATTTGTATATATTTTGTTCCACTTTCAATTTGGCTCTTTAACATTCTCTTCTCCCACCCATCTATTGAATAAAACTTATTAGGCGTTTGGAGCGTATAATTCGTTATTATAGTCAATGATTATTATAATTTGTAGGTTATTATAGACTGTGTTCGGAGATGTAGACTATTTTAGTTGGAGTTAATGAGGATGAGCAGAAAATTGTAAATACTGTAACAAAAAGGGATTTGAAATAGTAATACTATGGTTAATTATTAGTTATAATAGTTGAAAACACCAACTATTATAATTGAAAGCCCAAACAGTAAGTGAACTATTACAGCACACTCTCCCCAATTATAACTAAAAAAAATCTTCACACACCCACACATCTCACCTTAAATCATCAACATTAAAACCATCATGATAGTAAGAATAGCATCTTTAAACTATGGAAAAAAACATTAAAAACATCTTTATTGTTGAAATATATATAACAACAAAAATTACATAGTTTTCATACTTTCTATATAAAACATTTGAATTCTTAGATAATATCAAAAAAGCAAAAATAAATTTATGAAAACTACCTTCTTTTTTGTTTAGTTTTTAAATTTTGGCTTTTATTTTGAAAACAGTCTTAAAAAATAGATGACAAAACATAGAAACCGATAGATAGAAGTCATGTTTATAGACTTAATTTTCAAAAACACCAAAAACCAAAGACGACCTAAAATTAGTAGCTTTTAAAAAGTTGTTTTTTTTGTTTTTAGAAAATTTGGCTTAAAATTCAATTGTTTACTTATGAAAGATGAAATGGGGCCTTAAAACTTTGTTTTTACACAACTAAACGGTTTTCAAATGAAACTTTAAAATTTTCTTTTTCTTTTTGAAATTTAACTAAAAATTCGATCGTTTACATAAGATAGATGAAAATCATGGTAAAAAAAATAATGAGAAAATAAACACAATTTTCAAGAATAAAAAACCAAACCTCAAATGATTATCAAACAAATAGGTTTAGCTAAAATTAAATTGAGGATAAAAATTGCAATACTATTATTGAAGTCTACTATTTGAAATTTGATTGTTATGTCTATTTATTTTCTTAAACTTTAAAATCTTTATTTAATACATCGTGTCAAATCGAACAATGTTTATAAAATATATGGTACGCTTGCTAAGACGTTATTATACAAGGTTTATAGGTGTCAACAACAAATATTACGAAAGAGACACTACAAAGTTTAAAAATTAAATACACACAACACTATCAAAGTTGAATGAATATCTTAATAATATTAATAATAAAAATGAAATATGAATGATTGATGAATGTAAATGTGAACATTGTAGTAATAAATATGTATTAAATATTTTCCTATAAGTATTGAACTACTATAAAATACGTTGTGACAGCCATATTGAATATATATATATATTTTTTTTTTTTTTTTTTTTTTTAAAGTGTAGACTTGTAGCGAGATGGTAATGCCCATTGGCATTAGTGGAAAGGACAAAGACTCAATGTTTCCAGCTTCGCCATTCAATTTCAACGAATTCAAAAGCAATTGCAAGGCTTTGTATGGTGTCTCACCAAAGCGTCATTGGATCACCACTTTCTATGGAGGCCATGTAAACTCTTAACCTTTCATAGTCAACAGATTTTAGAGAAAAAAAATTAAGCAATAAATACTAATTTTTTTAGTTAAAGTGAACTTAACTCAATCGTAATTGATATGCAATTTCTCCTTAGAAGTCAAAAAGTCGGTCCCCTATCCCTGTCGTTGATGTACTCTAAAAAAAAATCTTTGTTTTTGGTTACCTAACTTGAGAGAGAAAAATATAAACAATCAAAATCATCTTCATTTAACCTAACTAGAGATAGATAGTGTATATTAGAAATAATTTCTAAAAAAGAATATATTTTTAAATTGAAAACCAATTTTCCCATAAGCACTTTGGAAAACTTATTAACTAAAGTGTGTTTGCTATAATTTTTTAGGATAAAAAATGATTGTTTAATCCTTATAAATAAAAGGAAAATTTGAAAACTTGGATTAATTTAAAAAGCTATTTTTGAAAGAACTAGATCATCTAAAAATGACTTTTAAATTTTTAACCATAATATTTTAAATATATAATAACAAAAAAGATACATAATTGTGATTAATATAATCACGGTTGAATATAGTTCCATAATATGTTATTTAAAAAAAATGTAAAATATAATAATACATGGATTAAAAATAAAATACTTTCACTTTAAATTTCATTTAAGTCTATGTACTTTTAATAAATCGTAACTTTAGTCTTTTTTAAAGATAATTTTTAATTGAAATGGATCGAATGATAATCATAATTCTTATGCCAGAAAAAATATTATATAAATATTTTGTGAAATAATTCAACAAAAAAAATAATAACTTACAATTAAGATTTTTTTTTAATAAAAAACATAACTCATATGTTTATTGTAAAAAAAAAAAAAAAAAGTTGTCCTAATAATGAATAATTAGTGTAATTATTTAAAGTGGTAAATTTTGCAGGACATCAAATTAGTGCTTCATCGGTTTGCGAGTAACATTATTTTCTCTAACGGATTGAAAGATCCTTACAGCAGTGGCGGGTAAGAACATCAATAATATTTCTAATAGAAATTCTCTATTTCAATTTTGTTTGAATTTTGGCTAATTTTTATTTTGTTAGTTAGGTTCCCTAAATTTTCATCTTCAAATATAATTTTGTTTAGAAATCTCAACTTTCAAATATGTTTCATTTATAATTTTAAATTTTGCATTAAAGTATACTATATGTGTAACTTCAAATTTATTTCTACAAATAAGTAATCTAAAAAAAAAAAAAAAAATTAAATTTTCTTGTGTTTTATATTTTAAGTAAATATAATAAAATATTCGACAAGAGTGAAACGAAAAGTTGATTTTATTAAAGAAGGAAAAAAAGAAACAATAGAGAAAAAGAATAGAAAAGTTGACCCATACAAATGACATAATTATTTGGTAATAACTTTTAAATGGAATTTTTGTACCGATGACTTAAATTTAAGATGCCCCAATTTTCTAAAATAATAAAGGATGACTCATTGCAAAGAGTACAATAAAGGCGTGTTAATTATCAATGACTCATTGCAAAGAGTACAATAAAGGCGTGTTAATTATCAATATTGTCCCTCAAGCATCAGAATGACAAAAATTAAAACAAGATTTCCATTTTGTTGGAGATGAAAACTACTGTTAAGACGAATCTAATATGGTTTCTATGACAGGGTATTGCATAATATATCTCAGAGCGTTGTTGCTATTACCACTGCCAAAGGTATCTTTTTTATTCATATAGTTTCTAACTAATTTATTGATTCGGTCTTTTTGAGTTGAAATCAAATAAATAGTCGTGGCTTATTATTAAAGTGAGAGTTTTCATTTTCTCCACCTACTTTTTTTCTACTTATTTAGGAAGAATTGCATAATATAAAATTTTATTTTCTAGTAAGATTTTGGGTGAAGAATCGTTTTATTTAAGTGATTTTGAATTGATTTCAACTAATTCGATCTAAATTGATTTACTTATAAAAATTTAGTTTTAATGAATGCAATTTCAAAAGCGAATTTTAGTGTATTGTTTAATTATAAAGAAATTTTTAAAAATAGAAAAATAAGAGAAACTATTTACACAAAATAGCAAAATCTTTAGATAGTTGTAATAGAAGCTGATAGAAGTTCATCAGAGTCATGAGGCAAATGAAAATGAAATGTAAATTTGAAATGCAGGCTCCCATTGCTTAGACATAGGCACGGACAGGGAGGGTGATCCTGATTGGTTGATAAAGCAAAGGAAGGCTGAGATGGATATAATTGATGGTTGGATTTCTAAGTATCATGCTGATCTTTTGCTATTCAAGCAAAGTGTTGGTGCTGCCCATTAATATGTATTTCAAAAATGCATTTTGATTTCTAAAATGTAAGGTTATTTATATATTAAGTATTGAAGAACAAATCGCCAAAGTGAGCATAGCTCGACTGGCATATATAGTGTGTGGAATGAATTACATAAATAATGAAAAAATTGAAATATATATATTGAGAAACAAATGCGTA

mRNA sequence

ATGCATATGTACTGGAAAGGTATCGATCCCAAAACTCCCATCTTTGCTTTTCTCGGTGCTGAAAGTGATATCGACAATGACATACCTAGCGTTGGCTTTCCCCTCCATTTCGCCTCTCGATACAAGGCTATGTCGGTGTATTTAGAGCATCGATTTTATGGGAAATCAGTACCATTTGGTTCAATAGAGAAGGCAATGAAAAATGCTAGCATTCGAGGATACTTTAATTCAGCTCAAGCCTTAGCAGATTATGCTGACGTGCTTTTGAACATCAAGCAAAGGTTTGCATATGAGACTTCTCCAATTATAGTTATTGGAGCCTCCTATGGTGGAATGTTAGCTTCATGGTTCAGGCTAAAGTATCCTCATATCGCTCTTGGAGCCCTCGCTTCTTCAGCCCCAATCCTTTACTTTGACAACATCACACCTCAAGACGGATATTACTCCATCGTCTCCAAATCCTTCAAAGAAACAAGCAAAACATGTCACGACACCATACGCAGATCATGGGGTGAAATCGACCGAATCACTGAGAAAACTCCGGGAGGGCTTTCAATTCTAAGCAAGCGATTCAAAATTTGTGGGAAATTGAATATGAGTACGGATATCAAGAACCTTTTGGACAGTATGTATACATGGGCAGCTCAATACAACGATCCACATGATAAACCAGTGAGAACCATATGTGCAGCCATTGATGAAGAAGCCAAGAAAAAAAGTGATTTGATTCAGCAAGTGTTTGCAGGGCTTATTGCTGTCGTGGGGGAGAGATCTTGTTATGATACTTGTAGCGAGATGGTAATGCCCATTGGCATTAGTGGAAAGGACAAAGACTCAATGTTTCCAGCTTCGCCATTCAATTTCAACGAATTCAAAAGCAATTGCAAGGCTTTGTATGGTGTCTCACCAAAGCGTCATTGGATCACCACTTTCTATGGAGGCCATGACATCAAATTAGTGCTTCATCGGTTTGCGAGTAACATTATTTTCTCTAACGGATTGAAAGATCCTTACAGCAGTGGCGGGGTATTGCATAATATATCTCAGAGCGTTGTTGCTATTACCACTGCCAAAGGCTCCCATTGCTTAGACATAGGCACGGACAGGGAGGGTGATCCTGATTGGTTGATAAAGCAAAGGAAGGCTGAGATGGATATAATTGATGGTTGGATTTCTAAGTATCATGCTGATCTTTTGCTATTCAAGCAAAGTGTTGGTGCTGCCCATTAATATGTATTTCAAAAATGCATTTTGATTTCTAAAATGTAAGGTTATTTATATATTAAGTATTGAAGAACAAATCGCCAAAGTGAGCATAGCTCGACTGGCATATATAGTGTGTGGAATGAATTACATAAATAATGAAAAAATTGAAATATATATATTGAGAAACAAATGCGTA

Coding sequence (CDS)

ATGCATATGTACTGGAAAGGTATCGATCCCAAAACTCCCATCTTTGCTTTTCTCGGTGCTGAAAGTGATATCGACAATGACATACCTAGCGTTGGCTTTCCCCTCCATTTCGCCTCTCGATACAAGGCTATGTCGGTGTATTTAGAGCATCGATTTTATGGGAAATCAGTACCATTTGGTTCAATAGAGAAGGCAATGAAAAATGCTAGCATTCGAGGATACTTTAATTCAGCTCAAGCCTTAGCAGATTATGCTGACGTGCTTTTGAACATCAAGCAAAGGTTTGCATATGAGACTTCTCCAATTATAGTTATTGGAGCCTCCTATGGTGGAATGTTAGCTTCATGGTTCAGGCTAAAGTATCCTCATATCGCTCTTGGAGCCCTCGCTTCTTCAGCCCCAATCCTTTACTTTGACAACATCACACCTCAAGACGGATATTACTCCATCGTCTCCAAATCCTTCAAAGAAACAAGCAAAACATGTCACGACACCATACGCAGATCATGGGGTGAAATCGACCGAATCACTGAGAAAACTCCGGGAGGGCTTTCAATTCTAAGCAAGCGATTCAAAATTTGTGGGAAATTGAATATGAGTACGGATATCAAGAACCTTTTGGACAGTATGTATACATGGGCAGCTCAATACAACGATCCACATGATAAACCAGTGAGAACCATATGTGCAGCCATTGATGAAGAAGCCAAGAAAAAAAGTGATTTGATTCAGCAAGTGTTTGCAGGGCTTATTGCTGTCGTGGGGGAGAGATCTTGTTATGATACTTGTAGCGAGATGGTAATGCCCATTGGCATTAGTGGAAAGGACAAAGACTCAATGTTTCCAGCTTCGCCATTCAATTTCAACGAATTCAAAAGCAATTGCAAGGCTTTGTATGGTGTCTCACCAAAGCGTCATTGGATCACCACTTTCTATGGAGGCCATGACATCAAATTAGTGCTTCATCGGTTTGCGAGTAACATTATTTTCTCTAACGGATTGAAAGATCCTTACAGCAGTGGCGGGGTATTGCATAATATATCTCAGAGCGTTGTTGCTATTACCACTGCCAAAGGCTCCCATTGCTTAGACATAGGCACGGACAGGGAGGGTGATCCTGATTGGTTGATAAAGCAAAGGAAGGCTGAGATGGATATAATTGATGGTTGGATTTCTAAGTATCATGCTGATCTTTTGCTATTCAAGCAAAGTGTTGGTGCTGCCCATTAA

Protein sequence

MHMYWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIEKAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGGLSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAIDEEAKKKSDLIQQVFAGLIAVVGERSCYDTCSEMVMPIGISGKDKDSMFPASPFNFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLHNISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADLLLFKQSVGAAH
Homology
BLAST of Lsi03G000710 vs. ExPASy Swiss-Prot
Match: Q5RBU7 (Lysosomal Pro-X carboxypeptidase OS=Pongo abelii OX=9601 GN=PRCP PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 4.0e-64
Identity = 147/425 (34.59%), Postives = 224/425 (52.71%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           YWK       I  + G E DI     + GF    A   KAM V+ EHR+YG+S+PFG  +
Sbjct: 78  YWK--KNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFG--D 137

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRF-AYETSPIIVIGASYGGMLASWFRLKYP 123
              K++    +  S QALAD+A+++ ++K+     E  P+I IG SYGGMLA+WFR+KYP
Sbjct: 138 NTFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYP 197

Query: 124 HIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPG 183
           H+ +GALA+SAPI  F+++ P   +  IV+  F+++   C ++IRRSW  I+R++  T  
Sbjct: 198 HMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIRRSWDAINRLS-NTGS 257

Query: 184 GLSILSKRFKICGKLNMSTDIKNLLDSM-----------YTWAAQYNDPHDK-PVRTICA 243
           GL  L+    +C  L  S DI++L D +           Y +A+ +  P    P++ +C 
Sbjct: 258 GLQWLTGALHLCSPLT-SQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQ 317

Query: 244 AIDEEAKKKSDLIQQVFAGLIAVV---GERSC-----------------YDTCSEMVMPI 303
            +       S L+Q +F  L       G+  C                 Y  C+E+VMP 
Sbjct: 318 YLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPF 377

Query: 304 GISGKDKDSMFPASPFNFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIF 363
             +G   D MF    +N  E   +C   +GV P+  WITT YGG +I        +NI+F
Sbjct: 378 CTNG--VDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNIS-----SHTNIVF 437

Query: 364 SNGLKDPYSSGGVLHNISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGW 396
           SNG  DP+S GGV  +I+ ++VA+T ++G+H LD+ T    DP  ++  R  E+  +  W
Sbjct: 438 SNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPTSVLLARSLEVRHMKNW 489

BLAST of Lsi03G000710 vs. ExPASy Swiss-Prot
Match: P42785 (Lysosomal Pro-X carboxypeptidase OS=Homo sapiens OX=9606 GN=PRCP PE=1 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.0e-63
Identity = 146/425 (34.35%), Postives = 224/425 (52.71%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           YWK       I  + G E DI     + GF    A   KAM V+ EHR+YG+S+PFG  +
Sbjct: 78  YWK--KNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFG--D 137

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRF-AYETSPIIVIGASYGGMLASWFRLKYP 123
            + K++    +  S QALAD+A+++ ++K+     E  P+I IG SYGGMLA+WFR+KYP
Sbjct: 138 NSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYP 197

Query: 124 HIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPG 183
           H+ +GALA+SAPI  F+++ P   +  IV+  F+++   C ++I RSW  I+R++  T  
Sbjct: 198 HMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLS-NTGS 257

Query: 184 GLSILSKRFKICGKLNMSTDIKNLLDSM-----------YTWAAQYNDPHDK-PVRTICA 243
           GL  L+    +C  L  S DI++L D +           Y +A+ +  P    P++ +C 
Sbjct: 258 GLQWLTGALHLCSPLT-SQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQ 317

Query: 244 AIDEEAKKKSDLIQQVFAGLIAVV---GERSC-----------------YDTCSEMVMPI 303
            +       S L+Q +F  L       G+  C                 Y  C+E+VMP 
Sbjct: 318 YLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPF 377

Query: 304 GISGKDKDSMFPASPFNFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIF 363
             +G   D MF    +N  E   +C   +GV P+  WITT YGG +I        +NI+F
Sbjct: 378 CTNG--VDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNIS-----SHTNIVF 437

Query: 364 SNGLKDPYSSGGVLHNISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGW 396
           SNG  DP+S GGV  +I+ ++VA+T ++G+H LD+ T    DP  ++  R  E+  +  W
Sbjct: 438 SNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNW 489

BLAST of Lsi03G000710 vs. ExPASy Swiss-Prot
Match: Q2TA14 (Lysosomal Pro-X carboxypeptidase OS=Bos taurus OX=9913 GN=PRCP PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.8e-61
Identity = 145/428 (33.88%), Postives = 217/428 (50.70%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           YWK  +    I  + G E DI     + GF    A   KAM V+ EHR+YG+S+PFG+  
Sbjct: 80  YWK--EDGGSILFYTGNEGDIIWFCNNTGFMWDIAEEMKAMLVFAEHRYYGESLPFGA-- 139

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRF-AYETSPIIVIGASYGGMLASWFRLKYP 123
            +  ++    +  + QALAD+A ++  +K+         +I +G SYGGMLA+WFR+KYP
Sbjct: 140 DSFSDSRHLNFLTTEQALADFAKLIRYLKRTIPGARNQHVIALGGSYGGMLAAWFRMKYP 199

Query: 124 HIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPG 183
           H+ +GALASSAPI  F+++ P D +  IV+  F ++   C ++IRRSW  I+R+ +K   
Sbjct: 200 HLVVGALASSAPIWQFNDLVPCDIFMKIVTTDFSQSGPNCSESIRRSWDAINRLAKKGT- 259

Query: 184 GLSILSKRFKICGKLNMSTDIKNLLDSM-YTW--AAQYNDPHDK---------PVRTICA 243
           GL  LS+   +C  L  S D++ L D +  TW   A  + P++          PV+ +C 
Sbjct: 260 GLRWLSEALHLCTPLTKSQDVQRLKDWISETWVNVAMVDYPYESNFLQPLPAWPVKVVCQ 319

Query: 244 AIDEEAKKKSDLIQQVFAGLIAVV---GERSC-----------------YDTCSEMVMPI 303
                    + ++Q +F  L       G+  C                 Y  C+EMVMP 
Sbjct: 320 YFKYSNVPDTVMVQNIFQALNVYYNYSGQAKCLNVSETATSSLGVLGWSYQACTEMVMPT 379

Query: 304 GISGKDKDSMFPASPFNFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIF 363
              G   D MF    +N  E+  +C   +GV P+  WI T YGG +I        +NIIF
Sbjct: 380 CSDG--VDDMFEPHSWNMKEYSDDCFKQWGVRPRPSWIPTMYGGKNIS-----SHTNIIF 439

Query: 364 SNGLKDPYSSGGVLHNISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGW 399
           SNG  DP+S GGV  +I+ +++AI    G+H LD+      DP  +   R  E+  +  W
Sbjct: 440 SNGELDPWSGGGVTKDITDTLLAIVIPNGAHHLDLRASNALDPVSVQLTRSLEVKYMKQW 495

BLAST of Lsi03G000710 vs. ExPASy Swiss-Prot
Match: Q7TMR0 (Lysosomal Pro-X carboxypeptidase OS=Mus musculus OX=10090 GN=Prcp PE=1 SV=2)

HSP 1 Score: 233.0 bits (593), Expect = 5.9e-60
Identity = 136/417 (32.61%), Postives = 218/417 (52.28%), Query Frame = 0

Query: 14  IFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIEKAMKNASIRG 73
           I  + G E DI     + GF    A   KAM V+ EHR+YG+S+PFG  + + K++    
Sbjct: 84  ILFYTGNEGDIVWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFG--QDSFKDSQHLN 143

Query: 74  YFNSAQALADYADVLLNIKQRF-AYETSPIIVIGASYGGMLASWFRLKYPHIALGALASS 133
           +  S QALAD+A+++ ++++     +  P+I IG SYGGMLA+WFR+KYPHI +GALA+S
Sbjct: 144 FLTSEQALADFAELIRHLEKTIPGAQGQPVIAIGGSYGGMLAAWFRMKYPHIVVGALAAS 203

Query: 134 APILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGGLSILSKRFK 193
           API   D + P   +  IV+  F+++   C ++IR+SW  ID+++  +  GL  L+    
Sbjct: 204 APIWQLDGMVPCGEFMKIVTNDFRKSGPYCSESIRKSWNVIDKLS-GSGSGLQSLTNILH 263

Query: 194 ICGKLN----------MSTDIKNLLDSMYTWAAQYNDPHDK-PVRTICAAIDEEAKKKSD 253
           +C  L           ++    NL    Y +A  +  P    P++ +C  +       + 
Sbjct: 264 LCSPLTSEKIPTLKGWIAETWVNLAMVNYPYACNFLQPLPAWPIKEVCQYLKNPNVSDTV 323

Query: 254 LIQQVFAGLIAVV---GERSC-----------------YDTCSEMVMPIGISGKDKDSMF 313
           L+Q +F  L       G+ +C                 +  C+EMVMP   +G   D MF
Sbjct: 324 LLQNIFQALSVYYNYSGQAACLNISQTTTSSLGSMGWSFQACTEMVMPFCTNG--IDDMF 383

Query: 314 PASPFNFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSG 373
               ++  ++ ++C   +GV P+ HW+TT YGG +I        SNIIFSNG  DP+S G
Sbjct: 384 EPFLWDLEKYSNDCFNQWGVKPRPHWMTTMYGGKNIS-----SHSNIIFSNGELDPWSGG 443

Query: 374 GVLHNISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADL 399
           GV  +I+ ++VAI    G+H LD+      DP  ++  R  E+  +  WI  +++++
Sbjct: 444 GVTRDITDTLVAINIHDGAHHLDLRAHNAFDPSSVLLSRLLEVKHMKKWILDFYSNI 490

BLAST of Lsi03G000710 vs. ExPASy Swiss-Prot
Match: Q9ET22 (Dipeptidyl peptidase 2 OS=Mus musculus OX=10090 GN=Dpp7 PE=1 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 6.1e-49
Identity = 128/422 (30.33%), Postives = 203/422 (48.10%), Query Frame = 0

Query: 13  PIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIEKAMKNASIR 72
           PIF + G E DI +   + GF +  A++ +A+ V+ EHR+YGKS+PFG        ++ R
Sbjct: 80  PIFFYTGNEGDIWSFANNSGFMVELAAQQEALLVFAEHRYYGKSLPFG------VQSTQR 139

Query: 73  GY---FNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPHIALGAL 132
           GY       QALAD+A +L  ++Q      +P I  G SYGGML+++ R+KYPH+  GAL
Sbjct: 140 GYTQLLTVEQALADFAVLLQALRQDLGVHDAPTIAFGGSYGGMLSAYMRMKYPHLVAGAL 199

Query: 133 ASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGGLSILSK 192
           A+SAP++    +     ++  V+  F   S  C   +R ++ +I  +     G    +S+
Sbjct: 200 AASAPVVAVAGLGDSYQFFRDVTADFYGQSPKCAQAVRDAFQQIKDLF--LQGAYDTISQ 259

Query: 193 RFKICGKLNMSTDIKNLLD---SMYTWAAQYNDPHD---------KPVRTICAAIDEEAK 252
            F  C  L+   D+  L     + +T  A  + P+           PV+  C  +  E +
Sbjct: 260 NFGTCQSLSSPKDLTQLFGFARNAFTVLAMMDYPYPTDFLGPLPANPVKVGCQRLLNEGQ 319

Query: 253 KKSDLIQQVFAGLI-AVVGERSCYD--------------------------TCSEMVMPI 312
           +   L  +  AGL+    G   CYD                           C+E  + +
Sbjct: 320 RIMGL--RALAGLVYNSSGTEPCYDIYRLYQSCADPTGCGTGSDARAWDYQACTE--INL 379

Query: 313 GISGKDKDSMFPASPFNFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIF 372
                +   MFP  PF+    +  C   +GV P++ W+ T + G D+K      ASNIIF
Sbjct: 380 TFDSNNVTDMFPEIPFSEELRQQYCLDTWGVWPRQDWLQTSFWGGDLKA-----ASNIIF 439

Query: 373 SNGLKDPYSSGGVLHNISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGW 393
           SNG  DP++ GG+  N+S SV+A+T   G+H LD+      DP  +++ RK E  +I  W
Sbjct: 440 SNGDLDPWAGGGIQSNLSTSVIAVTIQGGAHHLDLRASNSEDPPSVVEVRKLESTLIREW 484

BLAST of Lsi03G000710 vs. ExPASy TrEMBL
Match: A0A6J1DJ73 (lysosomal Pro-X carboxypeptidase-like OS=Momordica charantia OX=3673 GN=LOC111020537 PE=3 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.4e-181
Identity = 314/419 (74.94%), Postives = 354/419 (84.49%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           YWKG++P TPIFAF GAE ++D+DIPS+G PL+FASRYKAM VYLEHRFYGKS+PFGS++
Sbjct: 81  YWKGVNPSTPIFAFFGAEENLDDDIPSIGLPLNFASRYKAMLVYLEHRFYGKSIPFGSLK 140

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           KAM+NA+IRGY +SAQALADYA VLL++K+ FA ETSPIIVIG SYGGMLASWFRLKYPH
Sbjct: 141 KAMENATIRGYLSSAQALADYAQVLLHVKKMFAAETSPIIVIGGSYGGMLASWFRLKYPH 200

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IALGALASSAPILYFDNITPQDGYYS+VSKSF+ETS+TC++ IRRSW EIDRI E+   G
Sbjct: 201 IALGALASSAPILYFDNITPQDGYYSVVSKSFRETSETCYEIIRRSWAEIDRIAEEKAQG 260

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAIDEEAKKK-SDL 243
           LSILSKRFK CGKLN S+++K+ LDS+ T AAQYN P   PV  ICAAID EAKKK SDL
Sbjct: 261 LSILSKRFKTCGKLNRSSELKDYLDSVLTEAAQYNFPSANPVDAICAAIDGEAKKKSSDL 320

Query: 244 IQQVFAGLIAVVGERSCYD---------------TCSEMVMPIGISGKDKDSMFPASPFN 303
           I QVFAG++A +GERSCYD                CSEMVMPIG SGK  DSMFP + F+
Sbjct: 321 IGQVFAGVVAYMGERSCYDVSDYDHDSTDQYSWQVCSEMVMPIGRSGKG-DSMFPTAQFD 380

Query: 304 FNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLHNI 363
            N FK++CKA YGVSPK HWITTFYGGHD+KLVLHRFASNIIFSNGLKDPYSSGGVLHNI
Sbjct: 381 LNSFKNDCKAWYGVSPKPHWITTFYGGHDLKLVLHRFASNIIFSNGLKDPYSSGGVLHNI 440

Query: 364 SQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADLLLFKQSVG 407
           SQ++VAITTAKG+HC+DI      DPDWL  QRK EMDIIDGWISKYHADLLLFK+SVG
Sbjct: 441 SQTIVAITTAKGAHCVDIQNAEVDDPDWLKMQRKTEMDIIDGWISKYHADLLLFKKSVG 498

BLAST of Lsi03G000710 vs. ExPASy TrEMBL
Match: A0A6J1FDJ0 (lysosomal Pro-X carboxypeptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111444393 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 3.5e-180
Identity = 307/421 (72.92%), Postives = 353/421 (83.85%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           +W+G++P  PI  F GAE DID+DI  + FP+ FASRYKAM VYLEHRFYGKSVPFGSIE
Sbjct: 80  HWQGVNPNAPIIVFFGAEEDIDSDISFIDFPIRFASRYKAMLVYLEHRFYGKSVPFGSIE 139

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           KAMKNAS+RG+ NSAQALADYA+VLL+IKQ FA +TSPIIVIG SYGGMLASWFRLKYPH
Sbjct: 140 KAMKNASVRGHLNSAQALADYAEVLLHIKQMFASQTSPIIVIGGSYGGMLASWFRLKYPH 199

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IALGALASSAPILYFDNITPQDGYYSIVSKSFKETS+TC++TIRRSW EIDRI +  P G
Sbjct: 200 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSQTCYETIRRSWAEIDRIAKNKPEG 259

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAIDEEAKKKSDLI 243
           L ILSKRFK C KLN S ++KN LD+++T+AAQYN+PH+KPVR ICAAIDE A+K SD+I
Sbjct: 260 LMILSKRFKTCKKLNESHELKNHLDNVFTYAAQYNEPHEKPVRGICAAIDEAARKNSDVI 319

Query: 244 QQVFAGLIAVVGERSCYD-----------------TCSEMVMPIGISGKDKDSMFPASPF 303
           +QV AG++A++GER CYD                   SEMVMPIGISGK+ DSMFP +PF
Sbjct: 320 EQVVAGVVALMGERDCYDVFEFGDPDNTIDPFTWQVYSEMVMPIGISGKE-DSMFPTAPF 379

Query: 304 NFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLHN 363
           +FN+FK +CKALYGVSP RHWITTFYGG D+KL+LHRF SNIIFSNGLKDPYSSGGVLHN
Sbjct: 380 DFNKFKEDCKALYGVSPNRHWITTFYGGQDLKLILHRFGSNIIFSNGLKDPYSSGGVLHN 439

Query: 364 ISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADLLLFKQSVG 408
           IS+S+VAI TA+GSHCLD+   RE DP+WL  QRKAEMDIID WISKY+ DLL   Q++G
Sbjct: 440 ISKSIVAIPTAEGSHCLDLQEGREDDPEWLTSQRKAEMDIIDVWISKYYVDLLQSNQTLG 499

BLAST of Lsi03G000710 vs. ExPASy TrEMBL
Match: A0A6J1FJ87 (lysosomal Pro-X carboxypeptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111444394 PE=3 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 2.0e-175
Identity = 299/421 (71.02%), Postives = 346/421 (82.19%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           +W+G++   PI    G+E D+++DI  + FP+  ASRYKAM VYLEHRFYGKSVPFGSIE
Sbjct: 81  HWQGVNLNAPIIVGFGSEGDVEDDISFLDFPIRLASRYKAMLVYLEHRFYGKSVPFGSIE 140

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           KAMKNAS+RGY NSAQALADYA++LL+IK+ FA +TSP IV+GASYGGMLASWFRLKYPH
Sbjct: 141 KAMKNASVRGYLNSAQALADYAEMLLHIKKMFASQTSPTIVMGASYGGMLASWFRLKYPH 200

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IALGALASSAPILYFDNITPQDGYYSIVSKSFKETS+TC++TIRRSW E+DRI    P G
Sbjct: 201 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSQTCYETIRRSWAEVDRIANNKPEG 260

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAIDEEAKKKSDLI 243
           L ILSKRFK C KLN S ++K  LD + T AAQYN+PH+KPVR ICAAIDE A+K SD+I
Sbjct: 261 LMILSKRFKTCEKLNGSDELKYYLDHVLTSAAQYNNPHEKPVRGICAAIDEAARKNSDVI 320

Query: 244 QQVFAGLIAVVGERSCYDT-----------------CSEMVMPIGISGKDKDSMFPASPF 303
           +QV AG++A +GER CYD                   SE+VMPIGISGKDKDSMFP +PF
Sbjct: 321 EQVVAGVVAFMGERDCYDVFESGHPDNPIDPFTWQEYSEIVMPIGISGKDKDSMFPTAPF 380

Query: 304 NFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLHN 363
           +FN+FK +CKALYGVSP  HWITTFYGG D+KL+LHRF SNIIFSNGLKDPYSSGGVLHN
Sbjct: 381 DFNKFKKDCKALYGVSPNPHWITTFYGGQDLKLILHRFGSNIIFSNGLKDPYSSGGVLHN 440

Query: 364 ISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADLLLFKQSVG 408
           IS+S+VAI TA+GSHCLD+   RE DP+WLI QRKAEMDIID WISKY+ADLL   Q++G
Sbjct: 441 ISESIVAIPTAEGSHCLDLQEGREDDPEWLISQRKAEMDIIDAWISKYYADLLQSTQTLG 500

BLAST of Lsi03G000710 vs. ExPASy TrEMBL
Match: A0A6J1DHH2 (lysosomal Pro-X carboxypeptidase-like OS=Momordica charantia OX=3673 GN=LOC111020528 PE=3 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 4.5e-172
Identity = 300/419 (71.60%), Postives = 347/419 (82.82%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           YW+G++P  PIFA+LGAE  +DND+    F L FASRYKAM VYLEHRFYG+S+PFGS+E
Sbjct: 104 YWEGVNPNIPIFAYLGAEGSLDNDL---SFTLPFASRYKAMVVYLEHRFYGQSIPFGSLE 163

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           KAMKN +IRG+ +SAQALADYA V+L++K+ FA ETSPIIVIG SYGGMLASWFRLKYPH
Sbjct: 164 KAMKNTTIRGHLSSAQALADYAQVILHVKKMFAAETSPIIVIGGSYGGMLASWFRLKYPH 223

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IALGALASSAP+LYFDNITPQDGYYS+VSKSF+ETS+TC++ IRRSW EIDRI EK P G
Sbjct: 224 IALGALASSAPVLYFDNITPQDGYYSVVSKSFRETSETCYEIIRRSWAEIDRIAEK-PQG 283

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAIDEEAKKK-SDL 243
           LSILSKRFK C KLN S+D+K+ LD+M++ AAQYN P + PV  ICAAID EAKKK SDL
Sbjct: 284 LSILSKRFKTCAKLNRSSDLKDYLDNMFSGAAQYNFPSENPVDAICAAIDGEAKKKTSDL 343

Query: 244 IQQVFAGLIAVVGERSCYDT-----------------CSEMVMPIGISGKDKDSMFPASP 303
           I QVFAG++A +GE+ CYD                  CSEMV+PIG+SGK  +SMFP +P
Sbjct: 344 IGQVFAGVVAYMGEKPCYDVSDSWHTVDPTDQYSFQICSEMVIPIGVSGK-VESMFPTAP 403

Query: 304 FNFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLH 363
           F+ N FK++CKA YGVSPK HWITTFYGGHD+KLVL RFASNIIFSNGLKDPYSSGGVLH
Sbjct: 404 FDLNSFKNDCKAWYGVSPKPHWITTFYGGHDLKLVLQRFASNIIFSNGLKDPYSSGGVLH 463

Query: 364 NISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADLLLFKQS 405
           +IS+S+VA+TTAKG HCLDI +    DPDWLI QRK EMDIIDGWISKYHADLL FK+S
Sbjct: 464 DISESIVAVTTAKGCHCLDILSAEVDDPDWLIIQRKTEMDIIDGWISKYHADLLQFKKS 517

BLAST of Lsi03G000710 vs. ExPASy TrEMBL
Match: A0A0L9UN04 (Uncharacterized protein OS=Phaseolus angularis OX=3914 GN=LR48_Vigan05g173800 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 9.2e-149
Identity = 257/422 (60.90%), Postives = 321/422 (76.07%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           YW G     PIFAF GAE+ +D+D+  VGFP   A +++A+ VY+EHR+YGKS+PFGS E
Sbjct: 86  YWGGAKSSAPIFAFFGAEAPLDDDVYFVGFPRDNAPQFRALIVYIEHRYYGKSIPFGSRE 145

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           +AM+NAS RGYFNSAQA+ADYA VLL+IK+  + + SPIIV+G SYGGMLASWFRLKYPH
Sbjct: 146 EAMRNASTRGYFNSAQAIADYAAVLLHIKKTLSAQNSPIIVMGGSYGGMLASWFRLKYPH 205

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IALGALASSAPILYF+ I PQ GYY IV+K FKETS+TC+ TI +SW EIDR+ +K P G
Sbjct: 206 IALGALASSAPILYFNGIAPQAGYYYIVTKDFKETSETCYQTISKSWSEIDRVAKK-PNG 265

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAIDEEAKKKSDLI 243
           LSILSKRFK C KLN S ++K+ LDS+YT AAQY+ P + PV+ IC+AID  A KK+D++
Sbjct: 266 LSILSKRFKTCKKLNKSFELKDYLDSLYTDAAQYDFPSENPVKVICSAID-AAAKKTDIV 325

Query: 244 QQVFAGLIAVVGERSCYD-----------------TCSEMVMPIGISGKDKDSMFPASPF 303
            Q+F G++A +G RSCYD                 TCSEMVMPIG   +  DSMFP +PF
Sbjct: 326 GQIFEGVVAFMGHRSCYDMNEFNRPTETYIGWRWQTCSEMVMPIG--HERNDSMFPPAPF 385

Query: 304 NFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLHN 363
           N  +F   C +LYGV P+ HW+TT+YGG+D+KL+LHRFASNIIFSNGL+DPYSSGGVL N
Sbjct: 386 NMKKFVHECSSLYGVLPRPHWVTTYYGGYDLKLILHRFASNIIFSNGLRDPYSSGGVLEN 445

Query: 364 ISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADLLLFKQSVG 409
           IS SV+A+TT  G HCLDI +    DP+WL++QRK E+ I+ GWI++Y ADL+ F +   
Sbjct: 446 ISNSVLAVTTVNGCHCLDIQSRTAKDPEWLVRQRKEEVKIMKGWITEYEADLIAFSKQSK 503

BLAST of Lsi03G000710 vs. NCBI nr
Match: XP_031745605.1 (lysosomal Pro-X carboxypeptidase-like [Cucumis sativus] >KGN64893.2 hypothetical protein Csa_022850 [Cucumis sativus])

HSP 1 Score: 703.4 bits (1814), Expect = 1.2e-198
Identity = 334/423 (78.96%), Postives = 373/423 (88.18%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           YW+GI+PKTPIFA+LGAESDIDND+P VGFPL FAS+YKAMSVYLEHRFYGKS+PFGS+E
Sbjct: 85  YWEGINPKTPIFAYLGAESDIDNDVPYVGFPLRFASQYKAMSVYLEHRFYGKSIPFGSLE 144

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           KAMKN SIRGYFNSAQALADYA++LL+IK+ FAY+TSPIIV+GASYGGMLASWFRLKYPH
Sbjct: 145 KAMKNGSIRGYFNSAQALADYAELLLHIKKMFAYDTSPIIVMGASYGGMLASWFRLKYPH 204

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRI  KT GG
Sbjct: 205 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRIAGKTQGG 264

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAIDEEAKKKSDLI 243
           LSILSK+FK CGKL  S++IKNL+D+++T AAQYNDP++ PVR IC AIDEEAKKKS++I
Sbjct: 265 LSILSKQFKTCGKLKTSSEIKNLMDNVFTMAAQYNDPYENPVRGICVAIDEEAKKKSNVI 324

Query: 244 QQVFAGLIAVVGERSCYD-----------------TCSEMVMPIGISGKDKDSMFPASPF 303
           +QV AG+IA +GER CYD                  CSEMVMPIG SG+DK+SMFP SPF
Sbjct: 325 KQVVAGVIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGSSGRDKNSMFPPSPF 384

Query: 304 NFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLHN 363
            FN+FK+ CK LYGV+P+ HWITTFYGG DIKLVLHRF SNIIFSNGLKDPYSSGGVLHN
Sbjct: 385 RFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGLKDPYSSGGVLHN 444

Query: 364 ISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADLLLFKQSVG 410
           ISQ++VAITT KGSHCLDI ++RE DPDWLI QRK+EMDI+DGWISKY ADLLLF QSVG
Sbjct: 445 ISQTIVAITTPKGSHCLDIASEREDDPDWLITQRKSEMDIVDGWISKYQADLLLFNQSVG 504

BLAST of Lsi03G000710 vs. NCBI nr
Match: XP_031739623.1 (lysosomal Pro-X carboxypeptidase [Cucumis sativus] >KAE8649460.1 hypothetical protein Csa_018870 [Cucumis sativus])

HSP 1 Score: 699.9 bits (1805), Expect = 1.3e-197
Identity = 333/423 (78.72%), Postives = 371/423 (87.71%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           YW+GI+PKTPIFA+LGAESDIDND+P VGFPL FAS+YKAMSVYLEHRFYGKS+PFGS+E
Sbjct: 85  YWEGINPKTPIFAYLGAESDIDNDVPYVGFPLRFASQYKAMSVYLEHRFYGKSIPFGSLE 144

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           KAMKN SIRGYFNSAQALADYA++LL+IK+ FAY+TSPIIV+GASYGGMLASWFRLKYPH
Sbjct: 145 KAMKNGSIRGYFNSAQALADYAELLLHIKKMFAYDTSPIIVMGASYGGMLASWFRLKYPH 204

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRI  KT GG
Sbjct: 205 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRIAGKTRGG 264

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAIDEEAKKKSDLI 243
           LSILSK+FK CGKL  S++IKNL+DS++T AAQYNDP++ PVR IC AIDEEAKKKS++I
Sbjct: 265 LSILSKQFKTCGKLKTSSEIKNLMDSVFTMAAQYNDPYENPVRGICVAIDEEAKKKSNVI 324

Query: 244 QQVFAGLIAVVGERSCYD-----------------TCSEMVMPIGISGKDKDSMFPASPF 303
           +QV AG+IA +GER CYD                  CSEMVMPIG SG+DK+SMFP SPF
Sbjct: 325 KQVVAGVIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGSSGRDKNSMFPPSPF 384

Query: 304 NFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLHN 363
            FN+FK+ CK LYGV+P+ HWITTFYGG DIKLVLHRF SNIIFSNGLKDPYSSGGVLHN
Sbjct: 385 QFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGLKDPYSSGGVLHN 444

Query: 364 ISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADLLLFKQSVG 410
           ISQ++VAITT KGSHCLDI ++RE DPDWLI QRK+EMDI+DGWIS Y ADLLLF Q VG
Sbjct: 445 ISQTIVAITTPKGSHCLDIASEREDDPDWLITQRKSEMDIVDGWISTYQADLLLFNQRVG 504

BLAST of Lsi03G000710 vs. NCBI nr
Match: XP_022152926.1 (lysosomal Pro-X carboxypeptidase-like [Momordica charantia])

HSP 1 Score: 644.8 bits (1662), Expect = 5.0e-181
Identity = 314/419 (74.94%), Postives = 354/419 (84.49%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           YWKG++P TPIFAF GAE ++D+DIPS+G PL+FASRYKAM VYLEHRFYGKS+PFGS++
Sbjct: 81  YWKGVNPSTPIFAFFGAEENLDDDIPSIGLPLNFASRYKAMLVYLEHRFYGKSIPFGSLK 140

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           KAM+NA+IRGY +SAQALADYA VLL++K+ FA ETSPIIVIG SYGGMLASWFRLKYPH
Sbjct: 141 KAMENATIRGYLSSAQALADYAQVLLHVKKMFAAETSPIIVIGGSYGGMLASWFRLKYPH 200

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IALGALASSAPILYFDNITPQDGYYS+VSKSF+ETS+TC++ IRRSW EIDRI E+   G
Sbjct: 201 IALGALASSAPILYFDNITPQDGYYSVVSKSFRETSETCYEIIRRSWAEIDRIAEEKAQG 260

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAIDEEAKKK-SDL 243
           LSILSKRFK CGKLN S+++K+ LDS+ T AAQYN P   PV  ICAAID EAKKK SDL
Sbjct: 261 LSILSKRFKTCGKLNRSSELKDYLDSVLTEAAQYNFPSANPVDAICAAIDGEAKKKSSDL 320

Query: 244 IQQVFAGLIAVVGERSCYD---------------TCSEMVMPIGISGKDKDSMFPASPFN 303
           I QVFAG++A +GERSCYD                CSEMVMPIG SGK  DSMFP + F+
Sbjct: 321 IGQVFAGVVAYMGERSCYDVSDYDHDSTDQYSWQVCSEMVMPIGRSGKG-DSMFPTAQFD 380

Query: 304 FNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLHNI 363
            N FK++CKA YGVSPK HWITTFYGGHD+KLVLHRFASNIIFSNGLKDPYSSGGVLHNI
Sbjct: 381 LNSFKNDCKAWYGVSPKPHWITTFYGGHDLKLVLHRFASNIIFSNGLKDPYSSGGVLHNI 440

Query: 364 SQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADLLLFKQSVG 407
           SQ++VAITTAKG+HC+DI      DPDWL  QRK EMDIIDGWISKYHADLLLFK+SVG
Sbjct: 441 SQTIVAITTAKGAHCVDIQNAEVDDPDWLKMQRKTEMDIIDGWISKYHADLLLFKKSVG 498

BLAST of Lsi03G000710 vs. NCBI nr
Match: XP_022938254.1 (lysosomal Pro-X carboxypeptidase-like [Cucurbita moschata])

HSP 1 Score: 641.0 bits (1652), Expect = 7.2e-180
Identity = 307/421 (72.92%), Postives = 353/421 (83.85%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           +W+G++P  PI  F GAE DID+DI  + FP+ FASRYKAM VYLEHRFYGKSVPFGSIE
Sbjct: 80  HWQGVNPNAPIIVFFGAEEDIDSDISFIDFPIRFASRYKAMLVYLEHRFYGKSVPFGSIE 139

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           KAMKNAS+RG+ NSAQALADYA+VLL+IKQ FA +TSPIIVIG SYGGMLASWFRLKYPH
Sbjct: 140 KAMKNASVRGHLNSAQALADYAEVLLHIKQMFASQTSPIIVIGGSYGGMLASWFRLKYPH 199

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IALGALASSAPILYFDNITPQDGYYSIVSKSFKETS+TC++TIRRSW EIDRI +  P G
Sbjct: 200 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSQTCYETIRRSWAEIDRIAKNKPEG 259

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAIDEEAKKKSDLI 243
           L ILSKRFK C KLN S ++KN LD+++T+AAQYN+PH+KPVR ICAAIDE A+K SD+I
Sbjct: 260 LMILSKRFKTCKKLNESHELKNHLDNVFTYAAQYNEPHEKPVRGICAAIDEAARKNSDVI 319

Query: 244 QQVFAGLIAVVGERSCYD-----------------TCSEMVMPIGISGKDKDSMFPASPF 303
           +QV AG++A++GER CYD                   SEMVMPIGISGK+ DSMFP +PF
Sbjct: 320 EQVVAGVVALMGERDCYDVFEFGDPDNTIDPFTWQVYSEMVMPIGISGKE-DSMFPTAPF 379

Query: 304 NFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLHN 363
           +FN+FK +CKALYGVSP RHWITTFYGG D+KL+LHRF SNIIFSNGLKDPYSSGGVLHN
Sbjct: 380 DFNKFKEDCKALYGVSPNRHWITTFYGGQDLKLILHRFGSNIIFSNGLKDPYSSGGVLHN 439

Query: 364 ISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADLLLFKQSVG 408
           IS+S+VAI TA+GSHCLD+   RE DP+WL  QRKAEMDIID WISKY+ DLL   Q++G
Sbjct: 440 ISKSIVAIPTAEGSHCLDLQEGREDDPEWLTSQRKAEMDIIDVWISKYYVDLLQSNQTLG 499

BLAST of Lsi03G000710 vs. NCBI nr
Match: KAG6586010.1 (Lysosomal Pro-X carboxypeptidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 638.6 bits (1646), Expect = 3.6e-179
Identity = 306/421 (72.68%), Postives = 350/421 (83.14%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           +W+G++P  PI  F GAE DIDNDI  V FP+ FASRYKAM VYLEHRFYGKSVPFGSI+
Sbjct: 81  HWQGVNPNAPIVVFFGAEGDIDNDISLVDFPIRFASRYKAMLVYLEHRFYGKSVPFGSIK 140

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           KAMKNAS+RG+ NSAQALAD+A+VLL+IK+ FA +TSPIIVIG SYGGMLASWFRLKYPH
Sbjct: 141 KAMKNASVRGHLNSAQALADHAEVLLHIKKMFASQTSPIIVIGGSYGGMLASWFRLKYPH 200

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IALGALASSAPILYFDNITPQDGYYSIVSKSFKETS+TC++TIRRSW EIDRI +  P G
Sbjct: 201 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSQTCYETIRRSWAEIDRIAKNKPEG 260

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAIDEEAKKKSDLI 243
           L ILSKRFK C KLN S ++KN LD + T AAQYN+PH+KPVR ICAAIDE A+K SD+I
Sbjct: 261 LMILSKRFKTCEKLNGSHELKNHLDKVLTSAAQYNNPHEKPVRGICAAIDEAARKNSDVI 320

Query: 244 QQVFAGLIAVVGERSCYDT-----------------CSEMVMPIGISGKDKDSMFPASPF 303
           +QV AG++A +GER CYD                   SE+VMPIG+SGKDKDSMFP +PF
Sbjct: 321 EQVVAGVVAFMGERDCYDVFESGHPDNPIDPFTWQEYSEIVMPIGVSGKDKDSMFPTAPF 380

Query: 304 NFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLHN 363
           +FN+FK +CKALYGVSP  HWITTFYGG D+KL+LHRF SNIIFSNGLKDPYSSGGVLHN
Sbjct: 381 DFNKFKKDCKALYGVSPNPHWITTFYGGQDLKLILHRFGSNIIFSNGLKDPYSSGGVLHN 440

Query: 364 ISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADLLLFKQSVG 408
           IS+S+VAI TA+GSHCLD+    E DP+WLI QRKAEMDIID WISKY+ADLL   Q++G
Sbjct: 441 ISESIVAIPTAEGSHCLDLQEGMEDDPEWLISQRKAEMDIIDAWISKYYADLLQSNQTLG 500

BLAST of Lsi03G000710 vs. TAIR 10
Match: AT5G22860.1 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 457.6 bits (1176), Expect = 1.0e-128
Identity = 220/413 (53.27%), Postives = 294/413 (71.19%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           +W G     PI AFLG ES +D+D+ ++GF      R  A+ VY+EHR+YG+++PFGS E
Sbjct: 86  HWGGAKANAPILAFLGEESSLDSDLAAIGFLRDNGPRLNALLVYIEHRYYGETMPFGSAE 145

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           +A+KNAS  GY N+AQALADYA +LL++K++++   SPIIVIG SYGGMLA+WFRLKYPH
Sbjct: 146 EALKNASTLGYLNAAQALADYAAILLHVKEKYSTNHSPIIVIGGSYGGMLAAWFRLKYPH 205

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IALGALASSAP+LYF++  P+ GYY IV+K FKE S+ C++TIR SW EIDR+  K P G
Sbjct: 206 IALGALASSAPLLYFEDTRPKFGYYYIVTKVFKEASERCYNTIRNSWIEIDRVAGK-PNG 265

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAID-EEAKKKSDL 243
           LSILSK+FK C  LN S DIK+ LD++Y  A QYN   +  V  +C AI+     ++ +L
Sbjct: 266 LSILSKQFKTCAPLNGSFDIKDFLDTIYAEAVQYNRGPNFWVAKVCNAINANPPNRRYNL 325

Query: 244 IQQVFAGLIAVVGERSCYDT-----------------CSEMVMPIGISGKDKDSMFPASP 303
           + ++FAG++A+VG R+CYDT                 CSE+VMP+G     +D+MFP +P
Sbjct: 326 LDRIFAGVVALVGNRTCYDTKMFAQPTNNNIAWRWQSCSEIVMPVGYD--KQDTMFPTAP 385

Query: 304 FNFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLH 363
           FN   +   CK+ +GV+P+ HWITT++G  ++KL+L +F SNIIFSNGL DPYS GGVL 
Sbjct: 386 FNMTSYIDGCKSYHGVTPRPHWITTYFGIQEVKLILQKFGSNIIFSNGLSDPYSVGGVLE 445

Query: 364 NISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADL 399
           +IS ++VAITT  GSHCLDI    + DP+WL+ QR+ E+ +ID WIS Y  DL
Sbjct: 446 DISDTLVAITTKNGSHCLDITLKSKEDPEWLVIQREKEIKVIDSWISTYQNDL 495

BLAST of Lsi03G000710 vs. TAIR 10
Match: AT5G22860.2 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 391.0 bits (1003), Expect = 1.2e-108
Identity = 189/357 (52.94%), Postives = 254/357 (71.15%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           +W G     PI AFLG ES +D+D+ ++GF      R  A+ VY+EHR+YG+++PFGS E
Sbjct: 86  HWGGAKANAPILAFLGEESSLDSDLAAIGFLRDNGPRLNALLVYIEHRYYGETMPFGSAE 145

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           +A+KNAS  GY N+AQALADYA +LL++K++++   SPIIVIG SYGGMLA+WFRLKYPH
Sbjct: 146 EALKNASTLGYLNAAQALADYAAILLHVKEKYSTNHSPIIVIGGSYGGMLAAWFRLKYPH 205

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IALGALASSAP+LYF++  P+ GYY IV+K FKE S+ C++TIR SW EIDR+  K P G
Sbjct: 206 IALGALASSAPLLYFEDTRPKFGYYYIVTKVFKEASERCYNTIRNSWIEIDRVAGK-PNG 265

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAAID-EEAKKKSDL 243
           LSILSK+FK C  LN S DIK+ LD++Y  A QYN   +  V  +C AI+     ++ +L
Sbjct: 266 LSILSKQFKTCAPLNGSFDIKDFLDTIYAEAVQYNRGPNFWVAKVCNAINANPPNRRYNL 325

Query: 244 IQQVFAGLIAVVGERSCYDT-----------------CSEMVMPIGISGKDKDSMFPASP 303
           + ++FAG++A+VG R+CYDT                 CSE+VMP+G     +D+MFP +P
Sbjct: 326 LDRIFAGVVALVGNRTCYDTKMFAQPTNNNIAWRWQSCSEIVMPVGYD--KQDTMFPTAP 385

Query: 304 FNFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGG 343
           FN   +   CK+ +GV+P+ HWITT++G  ++KL+L +F SNIIFSNGL DPYS GG
Sbjct: 386 FNMTSYIDGCKSYHGVTPRPHWITTYFGIQEVKLILQKFGSNIIFSNGLSDPYSVGG 439

BLAST of Lsi03G000710 vs. TAIR 10
Match: AT2G24280.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 341.7 bits (875), Expect = 8.4e-94
Identity = 179/413 (43.34%), Postives = 245/413 (59.32%), Query Frame = 0

Query: 13  PIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIEKAMKNASIR 72
           PIF + G E DID    + GF L  A +++A+ V++EHRFYG+S PFG  +K+ K+A   
Sbjct: 84  PIFVYTGNEGDIDWFASNTGFMLDIAPKFRALLVFIEHRFYGESTPFG--KKSHKSAETL 143

Query: 73  GYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPHIALGALASS 132
           GY NS QALADYA ++ ++KQ  + E SP++V G SYGGMLA+WFRLKYPHI +GALASS
Sbjct: 144 GYLNSQQALADYAILIRSLKQNLSSEASPVVVFGGSYGGMLAAWFRLKYPHITIGALASS 203

Query: 133 APILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGGLSILSKRFK 192
           APIL+FDNI P   +Y  +S+ FK+ S  C   I+RSW E++ ++     GL  LSK+F+
Sbjct: 204 APILHFDNIVPLTSFYDAISQDFKDASINCFKVIKRSWEELEAVS-TMKNGLQELSKKFR 263

Query: 193 ICGKLNMSTDIKNLLDSMYTWAAQYNDP---------HDKPVRTICAAIDEEAKKKSDLI 252
            C  L+     ++ L   + + A  N P            PV  +C  ID   +  S+L 
Sbjct: 264 TCKGLHSQYSARDWLSGAFVYTAMVNYPTAANFMAPLPGYPVEQMCKIIDGFPRGSSNLD 323

Query: 253 QQVFAGLI--AVVGERSC----------------YDTCSEMVMPIGISGKDKDSMFPASP 312
           +   A  +     G   C                Y  C+EMVMP+  S +   SM P   
Sbjct: 324 RAFAAASLYYNYSGSEKCFEMEQQTDDHGLDGWQYQACTEMVMPMSCSNQ---SMLPPYE 383

Query: 313 FNFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDPYSSGGVLH 372
            +   F+  C   YGV P+ HWITT +GG  I+ VL RF SNIIFSNG++DP+S GGVL 
Sbjct: 384 NDSEAFQEQCMTRYGVKPRPHWITTEFGGMRIETVLKRFGSNIIFSNGMQDPWSRGGVLK 443

Query: 373 NISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHADL 399
           NIS S+VA+ T KG+H  D+    + DP+WL +QR+ E+ II+ WIS+Y+ DL
Sbjct: 444 NISSSIVALVTKKGAHHADLRAATKDDPEWLKEQRRQEVAIIEKWISEYYRDL 490

BLAST of Lsi03G000710 vs. TAIR 10
Match: AT5G65760.1 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 335.1 bits (858), Expect = 7.8e-92
Identity = 169/420 (40.24%), Postives = 247/420 (58.81%), Query Frame = 0

Query: 4   YWKGIDPKTPIFAFLGAESDIDNDIPSVGFPLHFASRYKAMSVYLEHRFYGKSVPFGSIE 63
           +W G     PIF + G E DI+    + GF    A ++ A+ V+ EHR+YG+S+P+GS E
Sbjct: 88  HWLGASALGPIFLYCGNEGDIEWFATNSGFIWDIAPKFGALLVFPEHRYYGESMPYGSRE 147

Query: 64  KAMKNASIRGYFNSAQALADYADVLLNIKQRFAYETSPIIVIGASYGGMLASWFRLKYPH 123
           +A KNA+   Y  + QALAD+A  + ++K+  + E  P+++ G SYGGMLA+W RLKYPH
Sbjct: 148 EAYKNATTLSYLTTEQALADFAVFVTDLKRNLSAEACPVVLFGGSYGGMLAAWMRLKYPH 207

Query: 124 IALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWGEIDRITEKTPGG 183
           IA+GALASSAPIL F+++ P + +Y I S  FK  S +C +TI+ SW  I    +K   G
Sbjct: 208 IAIGALASSAPILQFEDVVPPETFYDIASNDFKRESSSCFNTIKDSWDAIIAEGQK-ENG 267

Query: 184 LSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPH---------DKPVRTICAAIDE 243
           L  L+K F  C  LN + D+ + LDS Y++ A  + P+           P+R +C  ID 
Sbjct: 268 LLQLTKTFHFCRVLNSTDDLSDWLDSAYSYLAMVDYPYPADFMMPLPGHPIREVCRKID- 327

Query: 244 EAKKKSDLIQQVFAGLIA---VVGERSCY--------------DTCSEMVMPIGISGKDK 303
            A   + ++ +++AG+       G   C+                C+EMVMP  +S   +
Sbjct: 328 GAGSNASILDRIYAGISVYYNYTGNVDCFKLDDDPHGLDGWNWQACTEMVMP--MSSNQE 387

Query: 304 DSMFPASPFNFNEFKSNCKALYGVSPKRHWITTFYGGHDIKLVLHRFASNIIFSNGLKDP 363
           +SMFP   FN++ +K  C   + V+P+  W+TT +GGHDI   L  F SNIIFSNGL DP
Sbjct: 388 NSMFPGYGFNYSSYKEECWNTFRVNPRPKWVTTEFGGHDIATTLKSFGSNIIFSNGLLDP 447

Query: 364 YSSGGVLHNISQSVVAITTAKGSHCLDIGTDREGDPDWLIKQRKAEMDIIDGWISKYHAD 398
           +S G VL N+S ++VA+ T +G+H LD+      DP WL+ QR+AE+ +I GWI  Y  +
Sbjct: 448 WSGGSVLKNLSDTIVALVTKEGAHHLDLRPSTPEDPKWLVDQREAEIRLIQGWIETYRVE 503

BLAST of Lsi03G000710 vs. TAIR 10
Match: AT3G28680.1 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 156.4 bits (394), Expect = 5.0e-38
Identity = 80/154 (51.95%), Postives = 106/154 (68.83%), Query Frame = 0

Query: 112 MLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWG 171
           +LA+WF+LKYP+IALGALASSAP+LYF++  P+ GY+ IV+K FKE SK CH+ I +SW 
Sbjct: 23  VLAAWFKLKYPYIALGALASSAPLLYFEDTLPKHGYFYIVTKVFKEMSKECHNKIHKSWD 82

Query: 172 EIDRITEKTPGGLSILSKRFKICGKLNMSTDIKNLLDSMYTWAAQYNDPHDKPVRTICAA 231
           EIDRI  K P  LSILSK FK+C  LN   ++K+ +  +Y   AQY+D +   V  +C A
Sbjct: 83  EIDRIAAK-PNSLSILSKNFKLCNPLNDIIELKSYVSYIYARTAQYSD-NQFSVARLCEA 142

Query: 232 ID-EEAKKKSDLIQQVFAGLIAVVGERSCYDTCS 265
           I+      KSDL+ Q+FAG++A  G  SCY   S
Sbjct: 143 INTSPPNTKSDLLDQIFAGVVASRGNISCYGMSS 174

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5RBU74.0e-6434.59Lysosomal Pro-X carboxypeptidase OS=Pongo abelii OX=9601 GN=PRCP PE=2 SV=1[more]
P427852.0e-6334.35Lysosomal Pro-X carboxypeptidase OS=Homo sapiens OX=9606 GN=PRCP PE=1 SV=1[more]
Q2TA141.8e-6133.88Lysosomal Pro-X carboxypeptidase OS=Bos taurus OX=9913 GN=PRCP PE=2 SV=1[more]
Q7TMR05.9e-6032.61Lysosomal Pro-X carboxypeptidase OS=Mus musculus OX=10090 GN=Prcp PE=1 SV=2[more]
Q9ET226.1e-4930.33Dipeptidyl peptidase 2 OS=Mus musculus OX=10090 GN=Dpp7 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1DJ732.4e-18174.94lysosomal Pro-X carboxypeptidase-like OS=Momordica charantia OX=3673 GN=LOC11102... [more]
A0A6J1FDJ03.5e-18072.92lysosomal Pro-X carboxypeptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111444... [more]
A0A6J1FJ872.0e-17571.02lysosomal Pro-X carboxypeptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111444... [more]
A0A6J1DHH24.5e-17271.60lysosomal Pro-X carboxypeptidase-like OS=Momordica charantia OX=3673 GN=LOC11102... [more]
A0A0L9UN049.2e-14960.90Uncharacterized protein OS=Phaseolus angularis OX=3914 GN=LR48_Vigan05g173800 PE... [more]
Match NameE-valueIdentityDescription
XP_031745605.11.2e-19878.96lysosomal Pro-X carboxypeptidase-like [Cucumis sativus] >KGN64893.2 hypothetical... [more]
XP_031739623.11.3e-19778.72lysosomal Pro-X carboxypeptidase [Cucumis sativus] >KAE8649460.1 hypothetical pr... [more]
XP_022152926.15.0e-18174.94lysosomal Pro-X carboxypeptidase-like [Momordica charantia][more]
XP_022938254.17.2e-18072.92lysosomal Pro-X carboxypeptidase-like [Cucurbita moschata][more]
KAG6586010.13.6e-17972.68Lysosomal Pro-X carboxypeptidase, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
AT5G22860.11.0e-12853.27Serine carboxypeptidase S28 family protein [more]
AT5G22860.21.2e-10852.94Serine carboxypeptidase S28 family protein [more]
AT2G24280.18.4e-9443.34alpha/beta-Hydrolases superfamily protein [more]
AT5G65760.17.8e-9240.24Serine carboxypeptidase S28 family protein [more]
AT3G28680.15.0e-3851.95Serine carboxypeptidase S28 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008758Peptidase S28PFAMPF05577Peptidase_S28coord: 259..381
e-value: 2.3E-13
score: 49.7
coord: 11..245
e-value: 6.4E-47
score: 160.3
IPR042269Serine carboxypeptidase S28, SKS domainGENE3D1.20.120.980Serine carboxypeptidase S28, SKS domaincoord: 143..285
e-value: 1.0E-100
score: 339.9
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 4..391
e-value: 1.0E-100
score: 339.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 46..361
NoneNo IPR availablePANTHERPTHR11010PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATEDcoord: 3..398
NoneNo IPR availablePANTHERPTHR11010:SF96PROLYLCARBOXYPEPTIDASE-LIKE PROTEIN-RELATEDcoord: 3..398

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G000710.1Lsi03G000710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004180 carboxypeptidase activity
molecular_function GO:0008236 serine-type peptidase activity