Lsi03G000580 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G000580
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionpheophorbide a oxygenase, chloroplastic
Locationchr03: 875888 .. 881891 (+)
RNA-Seq ExpressionLsi03G000580
SyntenyLsi03G000580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACACCCCTAGGTTAGCTCTGATTTGGGCTTCCAAGGATAGAAAATTTGTGCCATTTTCTCAATTTTCCTCTTCAATTCTCAATTGTCACGTTTCTCAAATTCTGTGGACATGATTCATCCATGGCGGAAACCCTGTTTGAACCCTCCGAATCATCTCCCTCCGTTTCCTGCGGGCCAGACGCCATTGGTTTGTTTGTTCAGAAAGCAACATCACTTTGCTTCTAGAAGAGAGTCCTAGAAATTTCAGCTTCAAACGTGTACGCAACATGTTCTAATTTGATCTACAGTCGTTAATTTTTTTTTCCTCCGTTTTAAATTTCATTCTCTGTGTTCGTTTTTGAATGAGCTTGGTTGTGTTTCTTTAATGGCTCTTTCGATCTCTACATCTGCTTTTTCTGCTACTCTTATTACATCCTCGTTAAATGATGTAAAATCTCTCAGAATAGTTCCTTCTTTAATCGCGGTGGATAGGAGCCCTAATTTTCCGTCGAGAAAATTGCACAGGAGGGCATCGGCTATCAGGCCTGTTCGAGTGGCTGCGCCTCCAACGCCGCCATCGACGGCAGATTCCGAGAAATCTGCGGCGGTGAATTCGGATGAAGTGGAAGTGGATGAATCGGGCAATGAGAGCTCGTCTTCGCCGTTTTCTTGGAGGGATAATTGGTACCCTGTTTCCCTGATTGAAGATTTGGACCCTGCTTTGCCAACCCCGTTTCAGCTTCTGGGAAGAGATATTGTTCTTTGGTTTGATAAATCAAGAGGCGAGTGGGTTGCTTTTGATGATAAATGCCCTCATCGTCTTGCTCCTTTGTCTGTAAGCTTCCTCACTTGTTCTTCCACATTTAGCATTGTGAAGCTGTTCTTTTTGATAAAATGGGAAAAATTAAGGAATGTAATGCAAAAAGTTTTTATTGGGTTTGTCCTTGAATTTGGTTTTTGCAAAAGAAAAGAAGTGAAACAAGTTAGGGGTTGATAATGTGAGTGCATTCTGGTCATCTAGCTCCAATAAGGTTAAAAAAATTGTGTAAAGTTGGAATCCTCTAGTCTCTCTGGTGAATTTGAGCGGAGCTTTGGGTTTGTGGTAGTGAAAAGAACTCAGCAGTTGATGCTCTTGCAATTCCTCTTTGCCCTAGGTCTAAAAGTTGTCCATTCTAATTTATAAAATTAGCTGAAAAAATTGAAACTTAAATGTACCCAAATGATCCCAACTCTGAACTCTAAATCTAGTCACCACAATAATTCTAATTTATGTGTGTATATATATTTTAGTTCAACCATTGTGGGGTTGGAGATGGAATTATTGACCTTTGAAATAGTAATTTATGCTAGTATGGATTAGCTATAATTTGTCAGTTGCAACTTGAAAAATACCTTGAATGGTTACAATTAAACTTTAGATTCTAAACCTTAAACAATCCATCTTAAAATTGTAAATCATTACCACCTCTAAATTATAGTTTTGTTACTTGTACCAATTATACCATCAAACTGATAAAATTGTTGCAATCAAACCCAACCTCTCACAAAGTTATAAGGGAGGGAAGGATTGAGATTGTGGTTGCAAGTGCAACAACTCCTATGGTTCAAAACTCCTTGCAATGTTTTCTCAAATAAAAAGGAGATAAATTGTCTGATGAGATCTTCCTCACTTTGATTAGATGTGATTTTTTAGGAAGGGCGGATAGATGAAGGTGGCAACTTGCAGTGCTCATACCATGGATGGTCCTTTGATGGGTGTGGATCTTGTGTTAAAATCCCCCAGGCGTCATCGGAAGGCCCAGAATCTCGGGCTAATCAGTCACCTAGAGCATGTGCTACTAGGTTTCCGACTCTGGTGTCTCAAGGCCTCCTCTTTGTTTGGCCAGATGAGAATGGTTGGGAACGAGCCAATGCCACTACACCTCCAAGGTAGTTGGTTCAGAATTTGCAGCTCCTTTTGTGTATCTTTCTTGATTAGAAAGTAACTTCACCTTTCCATCTATTATTTAACCGCAACGTGTGATCATTCCTTCATTATCACTGCAATTGTCTAGGCTCCCTGATGACTTTGATAAGCCTGAGTTCTCATCAGTTACGATTCAGCGGGATCTATTTTATGGTTATGATACACTAATGGAGAATGTCTCAGATCCTTCCCACATTGATTTTGCTCATCATAAGGTAAGGGAGGGCCCAAGTGAGAGAACCTGTTTCAATTAAACTTGAATTTTGCATCAGTGATTGAATTGTTATGCTTGTTTGTGTTTTTCTGTTCTTTCTGGTTCTGATGGATGTTTAGTGAAACAAAATGTGTCACATATAAAGAACCAAAAAACTTTGATGGTACCTGGTTGGTGTTGTTAAATATTTACTATGTTTTAGAAAACAAAATCAATTATGTTCCAAGAAATTAGAACAATTAATAGTTCTGATAGCAGTTCTTCAATCATACCAGAAAATTTGCCCTAGGAAACCCTATTTTTCTTTTGTTGAATATCTCAATGGCCTCTGAAAACGAACATCTTCACAGGTTACAGGAAGGCGGGATCGGGCAAAACCTTTACCTTTCAAGTTAGATTCTAGCGGTCCCTGGGGATTTTCTGGAGCAAATAAGGGTAACCCAAGGATCAGTGCCAAGTTTGTCGCTCCTTGTTACTATATCAACAAGTAAGGATTTCACTCTTTTTCACCCTACGTTCACATTTGAATAATGGATAGCTGGAATCATCCCTTGCACATTTTCTGATATTATATATCAATAATTATGCTTATGCAATTATGATGATTAGGTACCTCATGCGTTAAGCTTAACATTAGTGGGTAGCTTAGTACAATGCTGGTAGTTCATTGAGAGTGAGCTATCTCTCTCAAATCTATCCCCAAATTTGCTATGTCTTCTCCTCTATCTCCGTCTTCCTTTTTTTTTTTTTGAAAGACTCTCCGTCTTCCTTTTAAGCCTCTTACTTCTAACAACTTAACTAATTCACTTCTTTTTCCCAACCAGATGCATAATAAACTTCTTTTTAATTTCCCCCTGATCAGTAAATCCTAACCTAAAGTGCATGGCATAAGCTTCATCGCTACCAAAATGTCATTTTTACTATACATGTAAACTAATTGTTCAAGGATTGTGTAAGACCTCCAATCCTACATACGTATAAAAGAAAGGGTAAAAGAGGAAAAGAGAAAACAAATTAGAGAAAAGAAGAAGGGAGTTCGTCAGAAGAGAGTGGGGACCAAGGATGAGTAGGCTGGTGAGTGGGGTCCATTCGGGTGAGAGGAGGTTTAAGAAGGGTTTTTGGGGGTGGAAGAAGGCAGGCTGTTAGTAGGCAAGTTTTGCTTGGTGGAGAGGATGCCCAGCCCTCTTGAATGTGCTGGAGGCTAGGTTAATTGCTGTATTTCTTTCTTTGTCTCCTCTTATCTTCTTGATAGTCATATATTGTGTAATCACTCCTAAGAAGTTCATAAATAAGAAATATCAGAGTTATACTCTGTTCTTGGGAACCTTGTTGGGAATTTTTCTGTTTGCAGGAGAAGAATCCTAACAGATTGTTGAGTGAATCTGAAGCTGCAACTTACAAGCAATTCAATTTACTTGGATTTGAACATTGTATGACCAAGTATGAGTTACTGCTGAATATGGCAAGAAGTTTTAAGGCTTACTAATTAAGGGATCAATTAAGAAATGAATAATACTTACCTATTGATGGCCATCCATAATTTGATAGTTATTTATCTTTTGAGGAAAAAGTAAACAAAATCTCACCTTTTCTTTTTGCACGCTCTTCTAGATGAAAATTCTTAGTAATTGTAGTCGTAGACTGGTGAGGAGGTAAGAATTATTCTTAAGAAATTGTGTTGCGGTTGCAACACTGAATTTTAGTTTTGAGATTTCATTTTCAGCCAGATTTAGCTTTTTGTATGTTGTTACCTCACGTCTATGCTTTTATAACTATACCTTCAGAGGCAAGGTACATTCAACACCTGCAAGTTGATTGGAGAAAAGGAAAGATGCTAGTATGATCAAACTTGAGCGGTGAGGTAGGGGAATCTATTGAGATGCTTAGAGAAGAGAAAGGTGAATGAGCTCGGGGGAGGAAATTAAAGTTGCCAATGTATAATGGAGTAGGCTGGATGAGTGTTCCAAGCACAAGCGTACATTAGGTTTATTAATTTCCAAAGGAGGTTTAGACAGGGAGAGCTTTTAAGAATTTATATCCTCATTCCTTTCTACGCATGCAAGAAAGAACACAACGGGAGGTCTACTAAGTAGAGTTAGTTTCAACTTCAAGTATTCCGGCAAGGACCGTCAGACCGTTTTGTCGTACAGACAAGATAAGGCTGATAGTAGGAGGGGCATAACTGTTGTGGCGTAAGGAAGGGGAGCAGTGAATTGTAATAATTAACGAGAGGATCTTTAGTTTGTGAAAATATCAAACATCTCAAAAGTTTAGAATTCTTGGAGCTTCTTCTCATAAAACAATTACAAGTCCTTTTCTTTCATTCTCTAACATAATAATAGCTGCGCCCTACTGAGTTTATCGGCAACTGTTTCTCCATTCTATTTTGTTTTGCTTAAGTGCTAGACATTGTGAATCCCTCTTACACATATTCCTTTAGGTTTTAACTCTAATCTAAACTTTTCTTCAGAGTAGAGATTGACACAAAGCTTCCTTTACTTGGCGATCAAAAATGGGTAATATGGATTTGCTCCTTCAATGTGCCAATGGGTCCAGGAAAAACTCGTTCTATTGTTTGTAGTGCTCGAAATTTCTTCCAGTTTTCGATGCCCGGTCCTGCTTGGTGGCAGGTAAGCAGTCTGTGTTAATCAAATCCCTTCCTTATTTTTATGATATATTATCATCTCTATCATTATTGATCATGCAATCTGTAATTATATGTGATATTACTGTGTTTGACTTCCTAAGTTTGCTTTCTATTAGGTGGTTCCTCGATGGCACGAGCATTGGACATCAAATAAGGTATATGATGGAGACATGATTGTTCTTCAGGGTCAGGAGAAGATATTCTTGTCGATGGAAGGTTCGACAGATGTGAATAAAGAATACACTAAAATAACATTTACACCAACACAAGCAGATCGTTTGGTGCTTGCATTCCGAAATTGGCTGAGGCGGCACGGAAAGGGCCAACCTGAATGGTTTGGGGCTAGCAGCCAGCAGCCTCTGCCATCAACAGTTCTATCTAAACGTCAGGTACAGAAAATTACCAGCATCTGGATTGTTGTTATAAAACTATTGGCTAAGAAGCACAAACATTTTAGTTTGGCTGGAATGTCCGTGTCCAACACATATAGGACATTTCAAAACTTCGACAGTTGTTGGACATATATTGGACACTTATTAGTGTAAGATGTGTTTGACACTAGTTGTACAAAGTTAAAATAGGACCAACATTTGTTAGGCAGGCATCAAACACTTGTTACCATATACTTAATAGACACATATATGACATAAATAATAAAATTTGATAGCGAAATACATTAAGCTAAATTTTTTAAGAATGCAAAATGTATATAAACTCATTCACTTCGAATTTCCTGATTTAAAAATGATATATATTTTAAAAATGTATGTTTTAATAAACTTGTCCTTATCAAGTTTGTGTCATAAATTTTTAAAAAAGGACCTGTCGTTAAGTCCATGTTGTGTCGTATCAGTATGAATCATTCAATGTTTCCTGTTTAACAAGTTCAAAATGGGTAACTACTGTCTTTAGCTTTTTAGTTCTCTTTTTCTCCGCTACAGATGCTGGACAGATTTGAGCAACACACACTCCATTGTTCATCATGTAGAGGAGCTTATAAAACATTTCAGATACTGCAAAAGCTGCTAATTGGTGCGACTGTAGTTTTCGCAGCCACAGCCGGCATCCCTTCTGCTCTGCAGATACGTATTCTTGTCGCTGCTCTTGCACTCGCAAGCGCCGGTTTGGCTTACGCTCTGTTTGAACTTCAAAAGAATTTTGTGTTCATTGACTATGTTCATGCTGAGATCGATTAG

mRNA sequence

AAACACCCCTAGGTTAGCTCTGATTTGGGCTTCCAAGGATAGAAAATTTGTGCCATTTTCTCAATTTTCCTCTTCAATTCTCAATTGTCACGTTTCTCAAATTCTGTGGACATGATTCATCCATGGCGGAAACCCTGTTTGAACCCTCCGAATCATCTCCCTCCGTTTCCTGCGGGCCAGACGCCATTGGTTTGTTTGTTCAGAAAGCAACATCACTTTGCTTCTAGAAGAGAGTCCTAGAAATTTCAGCTTCAAACGTGTACGCAACATGTTCTAATTTGATCTACAGTCGTTAATTTTTTTTTCCTCCGTTTTAAATTTCATTCTCTGTGTTCGTTTTTGAATGAGCTTGGTTGTGTTTCTTTAATGGCTCTTTCGATCTCTACATCTGCTTTTTCTGCTACTCTTATTACATCCTCGTTAAATGATGTAAAATCTCTCAGAATAGTTCCTTCTTTAATCGCGGTGGATAGGAGCCCTAATTTTCCGTCGAGAAAATTGCACAGGAGGGCATCGGCTATCAGGCCTGTTCGAGTGGCTGCGCCTCCAACGCCGCCATCGACGGCAGATTCCGAGAAATCTGCGGCGGTGAATTCGGATGAAGTGGAAGTGGATGAATCGGGCAATGAGAGCTCGTCTTCGCCGTTTTCTTGGAGGGATAATTGGTACCCTGTTTCCCTGATTGAAGATTTGGACCCTGCTTTGCCAACCCCGTTTCAGCTTCTGGGAAGAGATATTGTTCTTTGGTTTGATAAATCAAGAGGCGAGTGGGTTGCTTTTGATGATAAATGCCCTCATCGTCTTGCTCCTTTGTCTGAAGGGCGGATAGATGAAGGTGGCAACTTGCAGTGCTCATACCATGGATGGTCCTTTGATGGGTGTGGATCTTGTGTTAAAATCCCCCAGGCGTCATCGGAAGGCCCAGAATCTCGGGCTAATCAGTCACCTAGAGCATGTGCTACTAGGTTTCCGACTCTGGTGTCTCAAGGCCTCCTCTTTGTTTGGCCAGATGAGAATGGTTGGGAACGAGCCAATGCCACTACACCTCCAAGGCTCCCTGATGACTTTGATAAGCCTGAGTTCTCATCAGTTACGATTCAGCGGGATCTATTTTATGGTTATGATACACTAATGGAGAATGTCTCAGATCCTTCCCACATTGATTTTGCTCATCATAAGGTTACAGGAAGGCGGGATCGGGCAAAACCTTTACCTTTCAAGTTAGATTCTAGCGGTCCCTGGGGATTTTCTGGAGCAAATAAGGGTAACCCAAGGATCAGTGCCAAGTTTGTCGCTCCTTGTTACTATATCAACAAAGTAGAGATTGACACAAAGCTTCCTTTACTTGGCGATCAAAAATGGGTAATATGGATTTGCTCCTTCAATGTGCCAATGGGTCCAGGAAAAACTCGTTCTATTGTTTGTAGTGCTCGAAATTTCTTCCAGTTTTCGATGCCCGGTCCTGCTTGGTGGCAGGTGGTTCCTCGATGGCACGAGCATTGGACATCAAATAAGGTATATGATGGAGACATGATTGTTCTTCAGGGTCAGGAGAAGATATTCTTGTCGATGGAAGCCACAGCCGGCATCCCTTCTGCTCTGCAGATACGTATTCTTGTCGCTGCTCTTGCACTCGCAAGCGCCGGTTTGGCTTACGCTCTGTTTGAACTTCAAAAGAATTTTGTGTTCATTGACTATGTTCATGCTGAGATCGATTAG

Coding sequence (CDS)

ATGGCTCTTTCGATCTCTACATCTGCTTTTTCTGCTACTCTTATTACATCCTCGTTAAATGATGTAAAATCTCTCAGAATAGTTCCTTCTTTAATCGCGGTGGATAGGAGCCCTAATTTTCCGTCGAGAAAATTGCACAGGAGGGCATCGGCTATCAGGCCTGTTCGAGTGGCTGCGCCTCCAACGCCGCCATCGACGGCAGATTCCGAGAAATCTGCGGCGGTGAATTCGGATGAAGTGGAAGTGGATGAATCGGGCAATGAGAGCTCGTCTTCGCCGTTTTCTTGGAGGGATAATTGGTACCCTGTTTCCCTGATTGAAGATTTGGACCCTGCTTTGCCAACCCCGTTTCAGCTTCTGGGAAGAGATATTGTTCTTTGGTTTGATAAATCAAGAGGCGAGTGGGTTGCTTTTGATGATAAATGCCCTCATCGTCTTGCTCCTTTGTCTGAAGGGCGGATAGATGAAGGTGGCAACTTGCAGTGCTCATACCATGGATGGTCCTTTGATGGGTGTGGATCTTGTGTTAAAATCCCCCAGGCGTCATCGGAAGGCCCAGAATCTCGGGCTAATCAGTCACCTAGAGCATGTGCTACTAGGTTTCCGACTCTGGTGTCTCAAGGCCTCCTCTTTGTTTGGCCAGATGAGAATGGTTGGGAACGAGCCAATGCCACTACACCTCCAAGGCTCCCTGATGACTTTGATAAGCCTGAGTTCTCATCAGTTACGATTCAGCGGGATCTATTTTATGGTTATGATACACTAATGGAGAATGTCTCAGATCCTTCCCACATTGATTTTGCTCATCATAAGGTTACAGGAAGGCGGGATCGGGCAAAACCTTTACCTTTCAAGTTAGATTCTAGCGGTCCCTGGGGATTTTCTGGAGCAAATAAGGGTAACCCAAGGATCAGTGCCAAGTTTGTCGCTCCTTGTTACTATATCAACAAAGTAGAGATTGACACAAAGCTTCCTTTACTTGGCGATCAAAAATGGGTAATATGGATTTGCTCCTTCAATGTGCCAATGGGTCCAGGAAAAACTCGTTCTATTGTTTGTAGTGCTCGAAATTTCTTCCAGTTTTCGATGCCCGGTCCTGCTTGGTGGCAGGTGGTTCCTCGATGGCACGAGCATTGGACATCAAATAAGGTATATGATGGAGACATGATTGTTCTTCAGGGTCAGGAGAAGATATTCTTGTCGATGGAAGCCACAGCCGGCATCCCTTCTGCTCTGCAGATACGTATTCTTGTCGCTGCTCTTGCACTCGCAAGCGCCGGTTTGGCTTACGCTCTGTTTGAACTTCAAAAGAATTTTGTGTTCATTGACTATGTTCATGCTGAGATCGATTAG

Protein sequence

MALSISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAPPTPPSTADSEKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLLGRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKGNPRISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEATAGIPSALQIRILVAALALASAGLAYALFELQKNFVFIDYVHAEID
Homology
BLAST of Lsi03G000580 vs. ExPASy Swiss-Prot
Match: Q9FYC2 (Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAO PE=1 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 6.7e-174
Identity = 309/546 (56.59%), Postives = 366/546 (67.03%), Query Frame = 0

Query: 5   ISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAPPTPP 64
           +S    S+T  T + +  K    +P L    + P   S    R      P+RVAAPP+ P
Sbjct: 1   MSVVLLSSTSATITKSQSKK---IPFLSPTTKFPLKVSISPSRSKLFHNPLRVAAPPSVP 60

Query: 65  STADSEKSAAVNSDEVEVDESGN-ESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLLGRD 124
           ++  +E+        +E +  G+ E   S F WRD+WYPVSL+EDLDP +PTPFQLLGRD
Sbjct: 61  TSDSTEEK------RIEEEYGGDKEEEGSEFKWRDHWYPVSLVEDLDPNVPTPFQLLGRD 120

Query: 125 IVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQASS 184
           +VLWFD++  +W AFDD CPHRLAPLSEGR+DE G+LQCSYHGWSF GCGSC +IPQA++
Sbjct: 121 LVLWFDRNDQKWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWSFGGCGSCTRIPQAAT 180

Query: 185 EGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFSSVT 244
            GPE+RA +SPRACA +FPT+VSQGLLFVWPDENGW+RAN+  PPRLPDDFDKPEFS+VT
Sbjct: 181 SGPEARAVKSPRACAIKFPTMVSQGLLFVWPDENGWDRANSIEPPRLPDDFDKPEFSTVT 240

Query: 245 IQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKGNPR 304
           IQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFK++SSGPWGF GAN  +PR
Sbjct: 241 IQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKVESSGPWGFQGANDDSPR 300

Query: 305 ISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSM 364
           I+AKFVAPCY +NK+E+D KLP++G+QKWVIWICSFN+PM PGKTRSIVCSARNFFQFS+
Sbjct: 301 ITAKFVAPCYSMNKIELDAKLPIVGNQKWVIWICSFNIPMAPGKTRSIVCSARNFFQFSV 360

Query: 365 PGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFL--SME------------------ 424
           PGPAWWQVVPRW+EHWTSN VYDGDMIVLQGQEK+FL  SME                  
Sbjct: 361 PGPAWWQVVPRWYEHWTSNLVYDGDMIVLQGQEKVFLAKSMESPDYDVNKQYTKLTFTPT 420

Query: 425 ------------------------------------------------------------ 451
                                                                       
Sbjct: 421 QADRFVLAFRNWLRRHGKSQPEWFGSTPSNQPLPSTVLTKRQMLDRFDQHTQVCSSCKGA 480

BLAST of Lsi03G000580 vs. ExPASy Swiss-Prot
Match: Q0DV66 (Pheophorbide a oxygenase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=PAO PE=2 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 2.2e-172
Identity = 304/522 (58.24%), Postives = 347/522 (66.48%), Query Frame = 0

Query: 26  RIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAPPTPPSTADSEKSAAVNSDEVEVDES 85
           R VPSL A+  S     R   RRA A   +RVAAPP+ P  AD               E 
Sbjct: 21  RRVPSLPALSAS----GRLRLRRARADTRLRVAAPPSVPGEADQAPGET---------EP 80

Query: 86  GNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLLGRDIVLWFDKSRGEWVAFDDKCPHR 145
              S+   F WRD+WYPVSL+EDLDP++PTPFQLL RD+V+W D   GEWVA DD+CPHR
Sbjct: 81  STSSADEKFVWRDHWYPVSLVEDLDPSVPTPFQLLNRDLVIWKDPKSGEWVALDDRCPHR 140

Query: 146 LAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQASSEGPESRANQSPRACATRFPTLV 205
           LAPLSEGRIDE G LQCSYHGWSFDG G+C +IPQA+ EGPE++A +SP+ACA +FPTLV
Sbjct: 141 LAPLSEGRIDETGCLQCSYHGWSFDGSGACTRIPQAAPEGPEAKAVRSPKACAIKFPTLV 200

Query: 206 SQGLLFVWPDENGWERANATTPPRLPDDFDKPEFSSVTIQRDLFYGYDTLMENVSDPSHI 265
           SQGLLFVWPDENGWE+A AT PP LP +F+ P FS+VTIQRDL+YGYDTLMENVSDPSHI
Sbjct: 201 SQGLLFVWPDENGWEKATATKPPMLPKEFEDPAFSTVTIQRDLYYGYDTLMENVSDPSHI 260

Query: 266 DFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKGNPRISAKFVAPCYYINKVEIDTKLP 325
           +FAHHKVTGRRDRA+PLPFK++SSG WG+SG+N GNPRISA FVAPCY +NK+EIDTKLP
Sbjct: 261 EFAHHKVTGRRDRARPLPFKMESSGAWGYSGSNSGNPRISATFVAPCYALNKIEIDTKLP 320

Query: 326 LLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHEHWTSNKVY 385
           + GDQKWVIWICSFN+PM PGKTRSIVCSARNFFQFSMPG AWWQ+VPRW+EHWTSN VY
Sbjct: 321 IFGDQKWVIWICSFNIPMAPGKTRSIVCSARNFFQFSMPGKAWWQLVPRWYEHWTSNLVY 380

Query: 386 DGDMIVLQGQEKIFLSME------------------------------------------ 445
           DGDMIVLQGQEKIFLS                                            
Sbjct: 381 DGDMIVLQGQEKIFLSASKESSADINQQYTKITFTPTQADRFVLAFRAWLRKFGNSQPDW 440

Query: 446 -------------------------------------------------------ATAGI 451
                                                                  ATAGI
Sbjct: 441 FGNPSQEVLPSTVLSKREMLDRYEQHTLKCSSCKGAYNAFQTLQKVFMGATVAFCATAGI 500

BLAST of Lsi03G000580 vs. ExPASy Swiss-Prot
Match: Q8W496 (Protochlorophyllide-dependent translocon component 52, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTC52 PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.7e-52
Identity = 139/445 (31.24%), Postives = 210/445 (47.19%), Query Frame = 0

Query: 12  ATLITSSLNDVKSLRIVPSLIAVDRSPNFP--SRKLHRRASAIRPVRVAAPPTPPSTADS 71
           A L   +L  ++ L   P       +P+ P     L  R S+     V++PP+       
Sbjct: 3   AALAACALPSLRILNTKPRFRCSFSNPSLPISPNSLITRKSSRFTTAVSSPPS------- 62

Query: 72  EKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLLGRDIVLWFD 131
             SAA +++     E+  E  S  F W  NWYPV  I DLD  +P   +++G D+V+W+D
Sbjct: 63  -SSAATSTNSPPEPEALFEPGSDKFDWYANWYPVMPICDLDKKVPHGKKVMGIDLVVWWD 122

Query: 132 KSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQASSEGPESR 191
           ++  +W   DD CPHRLAPLS+GRID+ G LQC YHGW F+G G C  IPQA  +GP   
Sbjct: 123 RNEKQWKVMDDTCPHRLAPLSDGRIDQWGRLQCVYHGWCFNGSGDCKLIPQAPPDGPP-- 182

Query: 192 ANQSPRACATRFPTLVSQGLLFVWPDENGWER--ANATTPPRLPDDFDKPEFSSVTIQRD 251
            +   +AC   +P+ V   +++ WP+ +   +       PP +P + + P F+ +   RD
Sbjct: 183 VHTFKQACVAVYPSTVQHEIIWFWPNSDPKYKNIIETNKPPYIP-ELEDPSFTKLMGNRD 242

Query: 252 LFYGYDTLMENVSDPSHIDFAHH------KVTGR-------------------------R 311
           + YGYD L+EN+ DP+H+ +AH+      K  G+                         R
Sbjct: 243 IPYGYDVLVENLMDPAHVPYAHYGLMRFPKPKGKYIICISNSCFNPFTNLQILLAEKIDR 302

Query: 312 DRAKPLPF---KLDSSG-----PWGFSGANKGNPRISAKFVAPCYY------INKVEIDT 371
           +  KPL     KLD+ G      WG+S            F+APC Y      + + E + 
Sbjct: 303 EGGKPLEINVKKLDNKGFFSKQEWGYS-----------NFIAPCVYRSSTDPLPEQEHEY 362

Query: 372 KLPLLGDQK-------WVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRW 401
             P   D+         +I+IC   +P+ PG++R I    RNF  F        ++VPRW
Sbjct: 363 PAPAASDKAALSKRRLSLIFIC---IPVSPGRSRLIWTFPRNFGVFID------KIVPRW 416

BLAST of Lsi03G000580 vs. ExPASy Swiss-Prot
Match: O49931 (Protein TIC 55, chloroplastic OS=Pisum sativum OX=3888 GN=TIC55 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.2e-37
Identity = 113/384 (29.43%), Postives = 184/384 (47.92%), Query Frame = 0

Query: 30  SLIAVDRSPNFPSRKL--HRRASAIRPVRVAAPPTPPSTADSEKSAAVNSDEVEVDESGN 89
           +L+  + S NF   K    RR      V  AA     +  D E+   V        E   
Sbjct: 31  TLLCTNPSSNFSFNKALSSRRRKQAWCVAAAADVKDATLLDGEEDQKVLVGPSSEQERKG 90

Query: 90  ESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLLGRDIVLWFDKSRGEWVAFDDKCPHRLA 149
           E   + + W + WYP+ L +++    P   ++  ++IVL+ D    ++  ++D+CPHRLA
Sbjct: 91  EREVADYDWTEEWYPLYLTKNVPHDAPLGLKVYDKNIVLFRD-GNDQFQCYEDRCPHRLA 150

Query: 150 PLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQASSEGPESRANQSPRACATRFPTLVSQ 209
            LSEG++ + G L+C YHGW F+G G CVKIPQ  ++     A     AC   +    SQ
Sbjct: 151 KLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQLPAD-----AKIPKSACVKTYEVRDSQ 210

Query: 210 GLLFVWPDENGWERANATTPP---RLP--DDFDKPEFSSVTIQRDLFYGYDTLMENVSDP 269
           G+L+VW         +  TPP   ++P  ++F +P F  ++   +L Y +  L+EN+ DP
Sbjct: 211 GVLWVW--------MSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDP 270

Query: 270 SHIDFAHHKV--TGRRDRAKPLPFKLDSSGPWGFSG----ANKGNPRISAKFVAPCYYIN 329
           +H+  +H +   + +R+ A+ L F++      GF+G       G+     +F APC   N
Sbjct: 271 AHVPISHDRTDWSAKREDAQALGFEVTERTDRGFAGWWGREKDGSKPNFLRFEAPCVLQN 330

Query: 330 KVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWH 389
             EI  K   +      +++C    P G GK+  IV      F  +   P   ++ P W+
Sbjct: 331 NREIVDKNGEINHFSG-LFLCR---PTGQGKSMLIV-----RFGATKRSPL-IKLFPEWY 389

Query: 390 EHWTSNKVYDGDMIVLQGQEKIFL 401
            H  ++KV++ DM  L  Q +I L
Sbjct: 391 FHQNASKVFEQDMGFLSSQNEILL 389

BLAST of Lsi03G000580 vs. ExPASy Swiss-Prot
Match: Q9SK50 (Protein TIC 55, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC55 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.7e-37
Identity = 107/361 (29.64%), Postives = 171/361 (47.37%), Query Frame = 0

Query: 61  PTPPSTADSEKSAAVNSDEVEVDESGNESSSSP----------FSWRDNWYPVSLIEDLD 120
           PT P      +SA   +   +  E G +   +P          + W + WYP+ L +++ 
Sbjct: 39  PTKPRLRLLRRSAVAGTAVSDQTEGGGDVLLNPEEEKRVEVADYDWTEEWYPLYLTKNVP 98

Query: 121 PALPTPFQLLGRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFD 180
              P    +  R IVL+ D   G    ++D+CPHRLA LSEG++ + G L+C YHGW F+
Sbjct: 99  EDAPLGLTVYDRQIVLYKD-GEGTLRCYEDRCPHRLAKLSEGQLID-GRLECLYHGWQFE 158

Query: 181 GCGSCVKIPQASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRL 240
           G G CVKIPQ  +     +A     AC   +    SQG+++VW             P +L
Sbjct: 159 GEGKCVKIPQLPASAKIPKA-----ACVKTYEVKDSQGVVWVW-----MSTKTPPNPEKL 218

Query: 241 P--DDFDKPEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKV--TGRRDRAKPLPFKL 300
           P  ++F +P F  ++   +L Y +  L+EN+ DP+H+  +H +   T +R+ A+PL F++
Sbjct: 219 PWFENFARPGFFDISTTHELPYDHSILLENLMDPAHVPISHDRTDFTAKREDAQPLVFEV 278

Query: 301 DSSGPWGFSGA-----NKGNPRISAKFVAPCYYINKVEIDTKLPLLGDQKWV--IWICSF 360
                 GF+G        G      +F APC   N  E + K    G + +   +++C  
Sbjct: 279 TERSNRGFAGTWGREKEGGKGSNLLRFDAPCVLQNNREFEGK---DGVKNYFSGLFLCR- 338

Query: 361 NVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIF 401
             P G GK+  IV      F  +   P    V+P+W  H  + KV++ DM  L  Q ++ 
Sbjct: 339 --PTGQGKSMLIV-----RFGVTKRSPL-VSVLPQWFWHQNACKVFEQDMGFLSSQNEVL 375

BLAST of Lsi03G000580 vs. ExPASy TrEMBL
Match: A0A5D3DBC9 (Pheophorbide a oxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold284G00270 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 6.6e-241
Identity = 424/545 (77.80%), Postives = 438/545 (80.37%), Query Frame = 0

Query: 1   MALSISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAP 60
           MALSISTS FS+TLITSSL DVKSLRIVPSLIAVDR+PNF  RKL   AS IRP+RVAAP
Sbjct: 1   MALSISTSLFSSTLITSSLYDVKSLRIVPSLIAVDRNPNFWLRKLRSTASCIRPLRVAAP 60

Query: 61  PTPPSTADSEKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPST DSEKS  V+SD+VEVDESGNESSS+PFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTVDSEKSEVVDSDDVEVDESGNESSSTPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKS GEWVAFDD+CPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSSGEWVAFDDRCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240
           ASSEGPESRA+QSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDS+GPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKF+APCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFIAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME----------------- 420
           FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME                 
Sbjct: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEGSTDVNKEYTKITFTPT 420

Query: 421 ------------------------------------------------------------ 451
                                                                       
Sbjct: 421 QADRLVLAFRNWLRRHGKGQPEWFRASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCKGAY 480

BLAST of Lsi03G000580 vs. ExPASy TrEMBL
Match: A0A1S3CII7 (pheophorbide a oxygenase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500813 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 6.6e-241
Identity = 424/545 (77.80%), Postives = 438/545 (80.37%), Query Frame = 0

Query: 1   MALSISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAP 60
           MALSISTS FS+TLITSSL DVKSLRIVPSLIAVDR+PNF  RKL   AS IRP+RVAAP
Sbjct: 1   MALSISTSLFSSTLITSSLYDVKSLRIVPSLIAVDRNPNFWLRKLRSTASCIRPLRVAAP 60

Query: 61  PTPPSTADSEKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPST DSEKS  V+SD+VEVDESGNESSS+PFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTVDSEKSEVVDSDDVEVDESGNESSSTPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKS GEWVAFDD+CPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSSGEWVAFDDRCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240
           ASSEGPESRA+QSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDS+GPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKF+APCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFIAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME----------------- 420
           FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME                 
Sbjct: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEGSTDVNKEYTKITFTPT 420

Query: 421 ------------------------------------------------------------ 451
                                                                       
Sbjct: 421 QADRLVLAFRNWLRRHGKGQPEWFRASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCKGAY 480

BLAST of Lsi03G000580 vs. ExPASy TrEMBL
Match: A0A6J1HMG2 (pheophorbide a oxygenase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111464914 PE=4 SV=1)

HSP 1 Score: 839.7 bits (2168), Expect = 5.6e-240
Identity = 426/545 (78.17%), Postives = 435/545 (79.82%), Query Frame = 0

Query: 1   MALSISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAP 60
           MALSI+TSA SATLITSSLN VKSLRIVPSLI  DR+PNF SRK H RAS IRPVRVAAP
Sbjct: 1   MALSIATSAVSATLITSSLNHVKSLRIVPSLIVSDRNPNFLSRKSHIRASTIRPVRVAAP 60

Query: 61  PTPPSTADSEKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPSTADSEKS A N DEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTADSEKSEAENLDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKSR EWVAFDDKCPHRLAPLSEGRIDE GNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSRSEWVAFDDKCPHRLAPLSEGRIDEDGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240
           ASSEGPESRA+QSPRACATRFPT+VSQGLLFVWPDENGWERANATTPPRLPDDF KPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTMVSQGLLFVWPDENGWERANATTPPRLPDDFYKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDS+GPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKFVAPCYYINKVEIDTKLP+LGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME----------------- 420
           FSMPGPAWWQ+VPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME                 
Sbjct: 361 FSMPGPAWWQLVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEDSTDVNKEYTKITFTPT 420

Query: 421 ------------------------------------------------------------ 451
                                                                       
Sbjct: 421 QADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRGAY 480

BLAST of Lsi03G000580 vs. ExPASy TrEMBL
Match: A0A6J1DFC6 (pheophorbide a oxygenase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111020548 PE=4 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 2.8e-239
Identity = 423/547 (77.33%), Postives = 434/547 (79.34%), Query Frame = 0

Query: 1   MALSISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAP 60
           MALSI+TSA SATLITSSL DVKS RIVP +IA DRSPNF +RKL  RA+A+RPVRVAAP
Sbjct: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60

Query: 61  PTPPSTADSEKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPSTADSEKSA  NSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240
           ASSEGPESRA+QSPRACATRFPTLVSQGLLFVWPDENGWER NAT PPRLPDDFDKPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDS+GPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKFVAPCYYINKVEIDTKLP+LGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS------------------- 420
           FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS                   
Sbjct: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420

Query: 421 ------------------------------------------------------------ 451
                                                                       
Sbjct: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480

BLAST of Lsi03G000580 vs. ExPASy TrEMBL
Match: A0A6J1HYR3 (pheophorbide a oxygenase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111469310 PE=4 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.5e-237
Identity = 422/545 (77.43%), Postives = 433/545 (79.45%), Query Frame = 0

Query: 1   MALSISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAP 60
           MALSI+TSA SATLITSSLN VKSLRIVPS+I  DR+PNF SRK H RASAIRPVRVAAP
Sbjct: 1   MALSIATSAVSATLITSSLNHVKSLRIVPSVIVSDRNPNFLSRKSHIRASAIRPVRVAAP 60

Query: 61  PTPPSTADSEKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPST DSEKS A N DEVEV E GNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTVDSEKSEAENLDEVEVVELGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKSR EWVAFDDKCPHRLAPLSEGRIDE GNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSRSEWVAFDDKCPHRLAPLSEGRIDEDGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240
           ASSEGPESRA+QSPRACATRFPT+VSQGLLFVWPDENGWERANATTPPRLPDDF KPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTMVSQGLLFVWPDENGWERANATTPPRLPDDFYKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDS+GPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKFVAPCYYINKVEIDTKLP++GDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFVAPCYYINKVEIDTKLPVVGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME----------------- 420
           FSMPGPAWWQ+VPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME                 
Sbjct: 361 FSMPGPAWWQLVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEDSTDVNKEYTKITFTPT 420

Query: 421 ------------------------------------------------------------ 451
                                                                       
Sbjct: 421 QADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRGAY 480

BLAST of Lsi03G000580 vs. NCBI nr
Match: XP_038894382.1 (pheophorbide a oxygenase, chloroplastic [Benincasa hispida])

HSP 1 Score: 859.0 bits (2218), Expect = 1.8e-245
Identity = 433/545 (79.45%), Postives = 441/545 (80.92%), Query Frame = 0

Query: 1   MALSISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAP 60
           MALSISTS FSATLITSSLNDVKSLRIVPSLIA DR+PNFP RKLH RAS IRPVRVAAP
Sbjct: 1   MALSISTSVFSATLITSSLNDVKSLRIVPSLIAADRNPNFPLRKLHGRASGIRPVRVAAP 60

Query: 61  PTPPSTADSEKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PT PSTA+SE S AVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTVPSTAESETSEAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKSRGEWVAFDD+CPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSRGEWVAFDDRCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240
           ASSEGPESRA+QSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDS+GPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKFVAPCYYINKVEIDTKLPL+GDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFVAPCYYINKVEIDTKLPLIGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME----------------- 420
           FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME                 
Sbjct: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEGSTDVNKEYTKITFTPT 420

Query: 421 ------------------------------------------------------------ 451
                                                                       
Sbjct: 421 QADRLVLAFRSWLRRHGKGQPEWFGASSNQPLPSTVLSKRQMLDRFEQHTLHCSSCKGAY 480

BLAST of Lsi03G000580 vs. NCBI nr
Match: XP_008462467.1 (PREDICTED: pheophorbide a oxygenase, chloroplastic [Cucumis melo] >KAA0032593.1 pheophorbide a oxygenase [Cucumis melo var. makuwa] >TYK20892.1 pheophorbide a oxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 842.8 bits (2176), Expect = 1.4e-240
Identity = 424/545 (77.80%), Postives = 438/545 (80.37%), Query Frame = 0

Query: 1   MALSISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAP 60
           MALSISTS FS+TLITSSL DVKSLRIVPSLIAVDR+PNF  RKL   AS IRP+RVAAP
Sbjct: 1   MALSISTSLFSSTLITSSLYDVKSLRIVPSLIAVDRNPNFWLRKLRSTASCIRPLRVAAP 60

Query: 61  PTPPSTADSEKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPST DSEKS  V+SD+VEVDESGNESSS+PFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTVDSEKSEVVDSDDVEVDESGNESSSTPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKS GEWVAFDD+CPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSSGEWVAFDDRCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240
           ASSEGPESRA+QSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDS+GPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKF+APCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFIAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME----------------- 420
           FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME                 
Sbjct: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEGSTDVNKEYTKITFTPT 420

Query: 421 ------------------------------------------------------------ 451
                                                                       
Sbjct: 421 QADRLVLAFRNWLRRHGKGQPEWFRASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCKGAY 480

BLAST of Lsi03G000580 vs. NCBI nr
Match: XP_022964963.1 (pheophorbide a oxygenase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 839.7 bits (2168), Expect = 1.2e-239
Identity = 426/545 (78.17%), Postives = 435/545 (79.82%), Query Frame = 0

Query: 1   MALSISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAP 60
           MALSI+TSA SATLITSSLN VKSLRIVPSLI  DR+PNF SRK H RAS IRPVRVAAP
Sbjct: 1   MALSIATSAVSATLITSSLNHVKSLRIVPSLIVSDRNPNFLSRKSHIRASTIRPVRVAAP 60

Query: 61  PTPPSTADSEKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPSTADSEKS A N DEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTADSEKSEAENLDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKSR EWVAFDDKCPHRLAPLSEGRIDE GNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSRSEWVAFDDKCPHRLAPLSEGRIDEDGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240
           ASSEGPESRA+QSPRACATRFPT+VSQGLLFVWPDENGWERANATTPPRLPDDF KPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTMVSQGLLFVWPDENGWERANATTPPRLPDDFYKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDS+GPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKFVAPCYYINKVEIDTKLP+LGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME----------------- 420
           FSMPGPAWWQ+VPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME                 
Sbjct: 361 FSMPGPAWWQLVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEDSTDVNKEYTKITFTPT 420

Query: 421 ------------------------------------------------------------ 451
                                                                       
Sbjct: 421 QADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRGAY 480

BLAST of Lsi03G000580 vs. NCBI nr
Match: XP_031740836.1 (pheophorbide a oxygenase, chloroplastic [Cucumis sativus] >KAE8652781.1 hypothetical protein Csa_022752 [Cucumis sativus])

HSP 1 Score: 837.4 bits (2162), Expect = 5.7e-239
Identity = 422/545 (77.43%), Postives = 434/545 (79.63%), Query Frame = 0

Query: 1   MALSISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAP 60
           MALSISTS FS+TLITSSL DVKSLRIVPSLIAVDR PNF  RK   RAS I PVRVAAP
Sbjct: 1   MALSISTSLFSSTLITSSLYDVKSLRIVPSLIAVDRDPNFRLRKFCGRASGISPVRVAAP 60

Query: 61  PTPPSTADSEKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPST DSE S  V SDEVEVDESGNE SS+PFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTVDSENSEVVESDEVEVDESGNEGSSTPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIV+WFDKSRGEWVAFDD+CPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVIWFDKSRGEWVAFDDRCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240
           ASSEGPESRA+QSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLD++GPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDTNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISA+FVAPCYYINKVEID+KLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAEFVAPCYYINKVEIDSKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME----------------- 420
           FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSME                 
Sbjct: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEGSTDVNKEYTKITFTPT 420

Query: 421 ------------------------------------------------------------ 451
                                                                       
Sbjct: 421 QADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCKGAY 480

BLAST of Lsi03G000580 vs. NCBI nr
Match: XP_022152935.1 (pheophorbide a oxygenase, chloroplastic [Momordica charantia])

HSP 1 Score: 837.4 bits (2162), Expect = 5.7e-239
Identity = 423/547 (77.33%), Postives = 434/547 (79.34%), Query Frame = 0

Query: 1   MALSISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAP 60
           MALSI+TSA SATLITSSL DVKS RIVP +IA DRSPNF +RKL  RA+A+RPVRVAAP
Sbjct: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60

Query: 61  PTPPSTADSEKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPSTADSEKSA  NSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240
           ASSEGPESRA+QSPRACATRFPTLVSQGLLFVWPDENGWER NAT PPRLPDDFDKPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDS+GPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKFVAPCYYINKVEIDTKLP+LGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS------------------- 420
           FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS                   
Sbjct: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420

Query: 421 ------------------------------------------------------------ 451
                                                                       
Sbjct: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480

BLAST of Lsi03G000580 vs. TAIR 10
Match: AT3G44880.1 (Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain )

HSP 1 Score: 611.7 bits (1576), Expect = 4.8e-175
Identity = 309/546 (56.59%), Postives = 366/546 (67.03%), Query Frame = 0

Query: 5   ISTSAFSATLITSSLNDVKSLRIVPSLIAVDRSPNFPSRKLHRRASAIRPVRVAAPPTPP 64
           +S    S+T  T + +  K    +P L    + P   S    R      P+RVAAPP+ P
Sbjct: 1   MSVVLLSSTSATITKSQSKK---IPFLSPTTKFPLKVSISPSRSKLFHNPLRVAAPPSVP 60

Query: 65  STADSEKSAAVNSDEVEVDESGN-ESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLLGRD 124
           ++  +E+        +E +  G+ E   S F WRD+WYPVSL+EDLDP +PTPFQLLGRD
Sbjct: 61  TSDSTEEK------RIEEEYGGDKEEEGSEFKWRDHWYPVSLVEDLDPNVPTPFQLLGRD 120

Query: 125 IVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQASS 184
           +VLWFD++  +W AFDD CPHRLAPLSEGR+DE G+LQCSYHGWSF GCGSC +IPQA++
Sbjct: 121 LVLWFDRNDQKWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWSFGGCGSCTRIPQAAT 180

Query: 185 EGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFSSVT 244
            GPE+RA +SPRACA +FPT+VSQGLLFVWPDENGW+RAN+  PPRLPDDFDKPEFS+VT
Sbjct: 181 SGPEARAVKSPRACAIKFPTMVSQGLLFVWPDENGWDRANSIEPPRLPDDFDKPEFSTVT 240

Query: 245 IQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSSGPWGFSGANKGNPR 304
           IQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFK++SSGPWGF GAN  +PR
Sbjct: 241 IQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKVESSGPWGFQGANDDSPR 300

Query: 305 ISAKFVAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSM 364
           I+AKFVAPCY +NK+E+D KLP++G+QKWVIWICSFN+PM PGKTRSIVCSARNFFQFS+
Sbjct: 301 ITAKFVAPCYSMNKIELDAKLPIVGNQKWVIWICSFNIPMAPGKTRSIVCSARNFFQFSV 360

Query: 365 PGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFL--SME------------------ 424
           PGPAWWQVVPRW+EHWTSN VYDGDMIVLQGQEK+FL  SME                  
Sbjct: 361 PGPAWWQVVPRWYEHWTSNLVYDGDMIVLQGQEKVFLAKSMESPDYDVNKQYTKLTFTPT 420

Query: 425 ------------------------------------------------------------ 451
                                                                       
Sbjct: 421 QADRFVLAFRNWLRRHGKSQPEWFGSTPSNQPLPSTVLTKRQMLDRFDQHTQVCSSCKGA 480

BLAST of Lsi03G000580 vs. TAIR 10
Match: AT4G25650.1 (ACD1-like )

HSP 1 Score: 216.1 bits (549), Expect = 5.8e-56
Identity = 138/422 (32.70%), Postives = 208/422 (49.29%), Query Frame = 0

Query: 12  ATLITSSLNDVKSLRIVPSLIAVDRSPNFP--SRKLHRRASAIRPVRVAAPPTPPSTADS 71
           A L   +L  ++ L   P       +P+ P     L  R S+     V++PP+       
Sbjct: 3   AALAACALPSLRILNTKPRFRCSFSNPSLPISPNSLITRKSSRFTTAVSSPPS------- 62

Query: 72  EKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLLGRDIVLWFD 131
             SAA +++     E+  E  S  F W  NWYPV  I DLD  +P   +++G D+V+W+D
Sbjct: 63  -SSAATSTNSPPEPEALFEPGSDKFDWYANWYPVMPICDLDKKVPHGKKVMGIDLVVWWD 122

Query: 132 KSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQASSEGPESR 191
           ++  +W   DD CPHRLAPLS+GRID+ G LQC YHGW F+G G C  IPQA  +GP   
Sbjct: 123 RNEKQWKVMDDTCPHRLAPLSDGRIDQWGRLQCVYHGWCFNGSGDCKLIPQAPPDGPP-- 182

Query: 192 ANQSPRACATRFPTLVSQGLLFVWPDENGWER--ANATTPPRLPDDFDKPEFSSVTIQRD 251
            +   +AC   +P+ V   +++ WP+ +   +       PP +P + + P F+ +   RD
Sbjct: 183 VHTFKQACVAVYPSTVQHEIIWFWPNSDPKYKNIIETNKPPYIP-ELEDPSFTKLMGNRD 242

Query: 252 LFYGYDTLMENVSDPSHIDFAHH--------KVTGRRDRAKPLPF---KLDSSG-----P 311
           + YGYD L+EN+ DP+H+ +AH+        K    R+  KPL     KLD+ G      
Sbjct: 243 IPYGYDVLVENLMDPAHVPYAHYGLMRFPKPKEKIDREGGKPLEINVKKLDNKGFFSKQE 302

Query: 312 WGFSGANKGNPRISAKFVAPCYY------INKVEIDTKLPLLGDQK-------WVIWICS 371
           WG+S            F+APC Y      + + E +   P   D+         +I+IC 
Sbjct: 303 WGYS-----------NFIAPCVYRSSTDPLPEQEHEYPAPAASDKAALSKRRLSLIFIC- 362

Query: 372 FNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKI 401
             +P+ PG++R I    RNF  F        ++VPRW  H   N + D D+ +L  +E+ 
Sbjct: 363 --IPVSPGRSRLIWTFPRNFGVFID------KIVPRWVFHIGQNTILDSDLHLLHVEERK 393

BLAST of Lsi03G000580 vs. TAIR 10
Match: AT4G25650.2 (ACD1-like )

HSP 1 Score: 208.4 bits (529), Expect = 1.2e-53
Identity = 139/445 (31.24%), Postives = 210/445 (47.19%), Query Frame = 0

Query: 12  ATLITSSLNDVKSLRIVPSLIAVDRSPNFP--SRKLHRRASAIRPVRVAAPPTPPSTADS 71
           A L   +L  ++ L   P       +P+ P     L  R S+     V++PP+       
Sbjct: 3   AALAACALPSLRILNTKPRFRCSFSNPSLPISPNSLITRKSSRFTTAVSSPPS------- 62

Query: 72  EKSAAVNSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLLGRDIVLWFD 131
             SAA +++     E+  E  S  F W  NWYPV  I DLD  +P   +++G D+V+W+D
Sbjct: 63  -SSAATSTNSPPEPEALFEPGSDKFDWYANWYPVMPICDLDKKVPHGKKVMGIDLVVWWD 122

Query: 132 KSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQASSEGPESR 191
           ++  +W   DD CPHRLAPLS+GRID+ G LQC YHGW F+G G C  IPQA  +GP   
Sbjct: 123 RNEKQWKVMDDTCPHRLAPLSDGRIDQWGRLQCVYHGWCFNGSGDCKLIPQAPPDGPP-- 182

Query: 192 ANQSPRACATRFPTLVSQGLLFVWPDENGWER--ANATTPPRLPDDFDKPEFSSVTIQRD 251
            +   +AC   +P+ V   +++ WP+ +   +       PP +P + + P F+ +   RD
Sbjct: 183 VHTFKQACVAVYPSTVQHEIIWFWPNSDPKYKNIIETNKPPYIP-ELEDPSFTKLMGNRD 242

Query: 252 LFYGYDTLMENVSDPSHIDFAHH------KVTGR-------------------------R 311
           + YGYD L+EN+ DP+H+ +AH+      K  G+                         R
Sbjct: 243 IPYGYDVLVENLMDPAHVPYAHYGLMRFPKPKGKYIICISNSCFNPFTNLQILLAEKIDR 302

Query: 312 DRAKPLPF---KLDSSG-----PWGFSGANKGNPRISAKFVAPCYY------INKVEIDT 371
           +  KPL     KLD+ G      WG+S            F+APC Y      + + E + 
Sbjct: 303 EGGKPLEINVKKLDNKGFFSKQEWGYS-----------NFIAPCVYRSSTDPLPEQEHEY 362

Query: 372 KLPLLGDQK-------WVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRW 401
             P   D+         +I+IC   +P+ PG++R I    RNF  F        ++VPRW
Sbjct: 363 PAPAASDKAALSKRRLSLIFIC---IPVSPGRSRLIWTFPRNFGVFID------KIVPRW 416

BLAST of Lsi03G000580 vs. TAIR 10
Match: AT2G24820.1 (translocon at the inner envelope membrane of chloroplasts 55-II )

HSP 1 Score: 157.9 bits (398), Expect = 1.9e-38
Identity = 107/361 (29.64%), Postives = 171/361 (47.37%), Query Frame = 0

Query: 61  PTPPSTADSEKSAAVNSDEVEVDESGNESSSSP----------FSWRDNWYPVSLIEDLD 120
           PT P      +SA   +   +  E G +   +P          + W + WYP+ L +++ 
Sbjct: 39  PTKPRLRLLRRSAVAGTAVSDQTEGGGDVLLNPEEEKRVEVADYDWTEEWYPLYLTKNVP 98

Query: 121 PALPTPFQLLGRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFD 180
              P    +  R IVL+ D   G    ++D+CPHRLA LSEG++ + G L+C YHGW F+
Sbjct: 99  EDAPLGLTVYDRQIVLYKD-GEGTLRCYEDRCPHRLAKLSEGQLID-GRLECLYHGWQFE 158

Query: 181 GCGSCVKIPQASSEGPESRANQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRL 240
           G G CVKIPQ  +     +A     AC   +    SQG+++VW             P +L
Sbjct: 159 GEGKCVKIPQLPASAKIPKA-----ACVKTYEVKDSQGVVWVW-----MSTKTPPNPEKL 218

Query: 241 P--DDFDKPEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKV--TGRRDRAKPLPFKL 300
           P  ++F +P F  ++   +L Y +  L+EN+ DP+H+  +H +   T +R+ A+PL F++
Sbjct: 219 PWFENFARPGFFDISTTHELPYDHSILLENLMDPAHVPISHDRTDFTAKREDAQPLVFEV 278

Query: 301 DSSGPWGFSGA-----NKGNPRISAKFVAPCYYINKVEIDTKLPLLGDQKWV--IWICSF 360
                 GF+G        G      +F APC   N  E + K    G + +   +++C  
Sbjct: 279 TERSNRGFAGTWGREKEGGKGSNLLRFDAPCVLQNNREFEGK---DGVKNYFSGLFLCR- 338

Query: 361 NVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIF 401
             P G GK+  IV      F  +   P    V+P+W  H  + KV++ DM  L  Q ++ 
Sbjct: 339 --PTGQGKSMLIV-----RFGVTKRSPL-VSVLPQWFWHQNACKVFEQDMGFLSSQNEVL 375

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FYC26.7e-17456.59Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAO P... [more]
Q0DV662.2e-17258.24Pheophorbide a oxygenase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947... [more]
Q8W4961.7e-5231.24Protochlorophyllide-dependent translocon component 52, chloroplastic OS=Arabidop... [more]
O499311.2e-3729.43Protein TIC 55, chloroplastic OS=Pisum sativum OX=3888 GN=TIC55 PE=1 SV=1[more]
Q9SK502.7e-3729.64Protein TIC 55, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC55 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DBC96.6e-24177.80Pheophorbide a oxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3CII76.6e-24177.80pheophorbide a oxygenase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500813 ... [more]
A0A6J1HMG25.6e-24078.17pheophorbide a oxygenase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1DFC62.8e-23977.33pheophorbide a oxygenase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1HYR31.5e-23777.43pheophorbide a oxygenase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111469... [more]
Match NameE-valueIdentityDescription
XP_038894382.11.8e-24579.45pheophorbide a oxygenase, chloroplastic [Benincasa hispida][more]
XP_008462467.11.4e-24077.80PREDICTED: pheophorbide a oxygenase, chloroplastic [Cucumis melo] >KAA0032593.1 ... [more]
XP_022964963.11.2e-23978.17pheophorbide a oxygenase, chloroplastic [Cucurbita moschata][more]
XP_031740836.15.7e-23977.43pheophorbide a oxygenase, chloroplastic [Cucumis sativus] >KAE8652781.1 hypothet... [more]
XP_022152935.15.7e-23977.33pheophorbide a oxygenase, chloroplastic [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G44880.14.8e-17556.59Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain [more]
AT4G25650.15.8e-5632.70ACD1-like [more]
AT4G25650.21.2e-5331.24ACD1-like [more]
AT2G24820.11.9e-3829.64translocon at the inner envelope membrane of chloroplasts 55-II [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017941Rieske [2Fe-2S] iron-sulphur domainPFAMPF00355Rieskecoord: 99..182
e-value: 4.4E-20
score: 71.4
IPR017941Rieske [2Fe-2S] iron-sulphur domainPROSITEPS51296RIESKEcoord: 100..212
score: 30.128016
NoneNo IPR availableGENE3D3.90.380.10coord: 238..412
e-value: 1.7E-7
score: 33.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 51..94
NoneNo IPR availablePANTHERPTHR21266IRON-SULFUR DOMAIN CONTAINING PROTEINcoord: 403..450
coord: 36..401
NoneNo IPR availablePANTHERPTHR21266:SF24PHEOPHORBIDE A OXYGENASE, CHLOROPLASTICcoord: 403..450
coord: 36..401
NoneNo IPR availableCDDcd03480Rieske_RO_Alpha_PaOcoord: 88..223
e-value: 8.45679E-87
score: 259.562
NoneNo IPR availableSUPERFAMILY55961Bet v1-likecoord: 207..401
IPR013626Pheophorbide a oxygenasePFAMPF08417PaOcoord: 308..401
e-value: 2.8E-23
score: 81.8
IPR036922Rieske [2Fe-2S] iron-sulphur domain superfamilyGENE3D2.102.10.10coord: 95..237
e-value: 1.6E-40
score: 139.9
IPR036922Rieske [2Fe-2S] iron-sulphur domain superfamilySUPERFAMILY50022ISP domaincoord: 94..219

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G000580.1Lsi03G000580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0031967 organelle envelope
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0010277 chlorophyllide a oxygenase [overall] activity
molecular_function GO:0046872 metal ion binding