Lsi02G029840 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G029840
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	clavaminate synthase-like protein At3g21360
Location	chr02: 35924432 .. 35927223 (-)
RNA-Seq Expression	Lsi02G029840
Synteny	Lsi02G029840

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCTCTAAGCCCAAACAAAGTGTTTAGCCGAGGCCCGATTGAATTGCCATTCATCAATTGCCAAGGCTGAAGCCCACAAAATCTAGCGTTCTCGTCGAACTTTTCGAACCTAGACGAAACCTCGTCCAGGTGGAAGCTTGCAGCCTGCCTACGCATTTCCCTCCACCACATAGAACCGCACAACGTCATCAGCTGGCGCGTCGAGAACAAGCGCCACCGTGAGCCCAAACCCAAAATCCAAGCGGCAGCGAATAGTTCCCGGGAATTTATCAGAAACTAACATGGCGGTAGAGAATTTCAAGGAAATTCAAATTCCGCATCAGAAACACTATCATGGAAGTTATTTCCCATTAGTTTTATCGCCGTCTTCGGCTTCTTTCAGCATTTCGTCCTTCACTCGAGCCATCAAGGCCCAGAAACCAATAATCGATTCCCTGATTCTCAAGTCCGGAGCAATACTCTTCAGAGGCTTTCCAGTAGAAGCGGCATCCGACTTCAACGACGTCGTTGAGGCCTTTGGTTACGAGGAGTTTCCGTACGTCGGAGGTGCAGCTCCTCGAACTAAGGTCGTTGGTCGGGTTTTCACGGCCAACGAGTCACCGCCGGACCAGAAGATCCCTTTTCATCACGAATTGGCCCAGGTACTCATTGTACTCTGTCTCACCTTTTTTAGCCTTTTGATTTTAGGGAATTCTACAAGATCTTTATAATTCATATCTATGTTTGGAAATTGATAAACACAATTTAGTTTGTAGCTTAGAATTAACAATTTAAATAAGGGGTACGATCTCTGAAATGGCTTGGGTTCGGTCGAAATAGAATCTACTTTACCACTTTGTAGGCTAATAAGAGCCTTTGTAGTTTTTTAGATACTCGAATTTTAACAACGGAAAGTCGTGGGTTGATGCTGTGTTTTGCTGGTGAAGTCTGAAATTGAACGGGGTTGGGGTTTTTGTATGTTTCAGGTGCCTGAATTCCCTGCCAAGCTGTTTTTCTATTGTGAAGTAAAGCCTAGAAGTGGTGGAGAAACGCCCATTGTTCTCAGTCATCTTGTTTATGAGAGAGTGAAGGAGAAATATCCAGAATTTGTTGAGAGGTTGGAGGAACAGGGCCTAATTTACACACGCGTGTTGGGAGAAGGTGATGATCCATCATCTCCAATTGGCCGTGGTTGGAAATCTACATTCTTGACGGGCGATAAGAGCATTGCTGAAGAAAGGTTCATAATCAAACCCAAACCCCCACCAAAGGGAAACTTTAACATTTGTTTAAAAAGCACCCTGGTTTTTTACTATTACCATTTAACCTTTCACAAAGGTTTCAAAACTATCCTTGGAATATAAATCCTTTAGAATTATAGACGAAAATCGGGGCTTAACACAGTGCAACGATGACCTAGAATGGCGTGGCAATCCAAATTTCTATTCTAGGTCACTTGCCGCATCGGTTATAGGTTCCATTTTTCAATCGAAATTCTGAAAGATTTATATTCTATAAATTATTTTGAAATGTTTAAGAATAATAAGGCTAATATTACAACTTTTGGAAGAATAAGAATATTTTCGACAGAAAGGGCAAAATTCAGAGTAGTTTTTATAATTTAACCCAAATGATTGATGCTGAAAAGTAGCTTATTTATAATATTATATTGGCTGTTTCACTGTTAGCTAACATTTAGATTTGCTGGGTGAATGCGCTTGAACATGGACTAAGAAAATTACTTCAAAGTGAGGACTGAGGAAATCCATGTACTGATGTTTTATTCGATTTGAAATCATGTTAGAATGCATAGAAGTTGCATATTTTCTAAGCAAGGAATTCGAGCTACTTGAAAAGATGAGGATTGATTTCCACGACAATAAAATGTGTTTCACTGCATGATAATAGAGCCTATGAACACTTGGTCTTACTAGAATCTACATGTTATTGTCATATATCGAACATTTTTGCACCTAACTTTTCTCACTATTGTCTTGTGTACTCTATCGTGTATTGCTCCAAAATGCTTTGTTTGTAGTTTGAACAAGAACTTCACATGACTAGTCGGTTTAACAAATCTAAACTTTCAACAGGGCAGCTAAGTTGGGAATGAAGTTGGAGTGGTTAAAAGATGGAGTGAAAACAGTTATGGGTCCAATCCCAGCCATCAAGTATGATGAAGTACGGCAGCGCAAGATTTGGTTTAACAGCATGGTTGCAGCTTACACCGGCTGGGAAGATGCGAGGAACGATCCTATAAAAGCGGTAACGTTTGGGGACGGTACTCCATTGCCAGCTGACATCATCCATGGCTGCTTGAGAATTCTGGAAGAGGAGTCTGTTGCCATTCCTTGGCAGAAGGGTGATGTTCTGCTGATAGACAATTGGGCCGTCCTTCACTCTCGTAGGCCATTCCTCCCTCCTCGCCGTATACTAGCTTCACTTTGCAGGTAAATTCATCACAAAGTCTTGAAACTTAGGGAAAATCATATAGGACCAATATTATGACAATTCTGAACCAGATGATTGATTTATTGACATCAAGTGAAATCAAATCAATGCTGCTCTTCAGCTGTCTATTTATTGAAGGTTCCCATGCTCTCTCTTTCACTCCAAATCCCTTCTCTCTCATGTAAACTAAACAATGTAATTAATGGAACTGTGTAGTGTTAATAATGTAACTGCCTCTGGCTTTCTTCAGTTAATTGTATAGTGTTTTATTGTAAGGGGATTTATCTCCAGCATTCATGGACGGGTTGCGGTTTGTGCTCGTTCAAAAACAGTAAAACATAAAATACAATATCATTCTCTA

mRNA sequence

TTTCTCTAAGCCCAAACAAAGTGTTTAGCCGAGGCCCGATTGAATTGCCATTCATCAATTGCCAAGGCTGAAGCCCACAAAATCTAGCGTTCTCGTCGAACTTTTCGAACCTAGACGAAACCTCGTCCAGGTGGAAGCTTGCAGCCTGCCTACGCATTTCCCTCCACCACATAGAACCGCACAACGTCATCAGCTGGCGCGTCGAGAACAAGCGCCACCGTGAGCCCAAACCCAAAATCCAAGCGGCAGCGAATAGTTCCCGGGAATTTATCAGAAACTAACATGGCGGTAGAGAATTTCAAGGAAATTCAAATTCCGCATCAGAAACACTATCATGGAAGTTATTTCCCATTAGTTTTATCGCCGTCTTCGGCTTCTTTCAGCATTTCGTCCTTCACTCGAGCCATCAAGGCCCAGAAACCAATAATCGATTCCCTGATTCTCAAGTCCGGAGCAATACTCTTCAGAGGCTTTCCAGTAGAAGCGGCATCCGACTTCAACGACGTCGTTGAGGCCTTTGGTTACGAGGAGTTTCCGTACGTCGGAGGTGCAGCTCCTCGAACTAAGGTCGTTGGTCGGGTTTTCACGGCCAACGAGTCACCGCCGGACCAGAAGATCCCTTTTCATCACGAATTGGCCCAGGTGCCTGAATTCCCTGCCAAGCTGTTTTTCTATTGTGAAGTAAAGCCTAGAAGTGGTGGAGAAACGCCCATTGTTCTCAGTCATCTTGTTTATGAGAGAGTGAAGGAGAAATATCCAGAATTTGTTGAGAGGGCAGCTAAGTTGGGAATGAAGTTGGAGTGGTTAAAAGATGGAGTGAAAACAGTTATGGGTCCAATCCCAGCCATCAAGTATGATGAAGTACGGCAGCGCAAGATTTGGTTTAACAGCATGGTTGCAGCTTACACCGGCTGGGAAGATGCGAGGAACGATCCTATAAAAGCGGTAACGTTTGGGGACGGTACTCCATTGCCAGCTGACATCATCCATGGCTGCTTGAGAATTCTGGAAGAGGAGTCTGTTGCCATTCCTTGGCAGAAGGGTGATGTTCTGCTGATAGACAATTGGGCCGTCCTTCACTCTCGTAGGCCATTCCTCCCTCCTCGCCGTATACTAGCTTCACTTTGCAGGTAAATTCATCACAAAGTCTTGAAACTTAGGGAAAATCATATAGGACCAATATTATGACAATTCTGAACCAGATGATTGATTTATTGACATCAAGTGAAATCAAATCAATGCTGCTCTTCAGCTGTCTATTTATTGAAGGTTCCCATGCTCTCTCTTTCACTCCAAATCCCTTCTCTCTCATGTAAACTAAACAATGTAATTAATGGAACTGTGTAGTGTTAATAATGTAACTGCCTCTGGCTTTCTTCAGTTAATTGTATAGTGTTTTATTGTAAGGGGATTTATCTCCAGCATTCATGGACGGGTTGCGGTTTGTGCTCGTTCAAAAACAGTAAAACATAAAATACAATATCATTCTCTA

Coding sequence (CDS)

ATGGCGGTAGAGAATTTCAAGGAAATTCAAATTCCGCATCAGAAACACTATCATGGAAGTTATTTCCCATTAGTTTTATCGCCGTCTTCGGCTTCTTTCAGCATTTCGTCCTTCACTCGAGCCATCAAGGCCCAGAAACCAATAATCGATTCCCTGATTCTCAAGTCCGGAGCAATACTCTTCAGAGGCTTTCCAGTAGAAGCGGCATCCGACTTCAACGACGTCGTTGAGGCCTTTGGTTACGAGGAGTTTCCGTACGTCGGAGGTGCAGCTCCTCGAACTAAGGTCGTTGGTCGGGTTTTCACGGCCAACGAGTCACCGCCGGACCAGAAGATCCCTTTTCATCACGAATTGGCCCAGGTGCCTGAATTCCCTGCCAAGCTGTTTTTCTATTGTGAAGTAAAGCCTAGAAGTGGTGGAGAAACGCCCATTGTTCTCAGTCATCTTGTTTATGAGAGAGTGAAGGAGAAATATCCAGAATTTGTTGAGAGGGCAGCTAAGTTGGGAATGAAGTTGGAGTGGTTAAAAGATGGAGTGAAAACAGTTATGGGTCCAATCCCAGCCATCAAGTATGATGAAGTACGGCAGCGCAAGATTTGGTTTAACAGCATGGTTGCAGCTTACACCGGCTGGGAAGATGCGAGGAACGATCCTATAAAAGCGGTAACGTTTGGGGACGGTACTCCATTGCCAGCTGACATCATCCATGGCTGCTTGAGAATTCTGGAAGAGGAGTCTGTTGCCATTCCTTGGCAGAAGGGTGATGTTCTGCTGATAGACAATTGGGCCGTCCTTCACTCTCGTAGGCCATTCCTCCCTCCTCGCCGTATACTAGCTTCACTTTGCAGGTAA

Protein sequence

MAVENFKEIQIPHQKHYHGSYFPLVLSPSSASFSISSFTRAIKAQKPIIDSLILKSGAILFRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQVPEFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFVERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKIWFNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLIDNWAVLHSRRPFLPPRRILASLCR

Homology

[Close]

BLAST of Lsi02G029840 vs. ExPASy Swiss-Prot
Match: Q9LIG0 (Clavaminate synthase-like protein At3g21360 OS=Arabidopsis thaliana OX=3702 GN=At3g21360 PE=1 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 5.4e-121
Identity = 214/323 (66.25%), Postives = 241/323 (74.61%), Query Frame = 0


Query: 8   EIQIPHQKHYHGSYFPLVLSPSSAS-----FSISSFTRAIKAQKPIIDSLILKSGAILFR 67
           E  IP QKHY    FP V+SP SAS      S+  FT+ IK QK  +DSL+ +SGA+LFR
Sbjct: 8   ETPIPQQKHYESKPFPAVISPPSASIPIPALSLPLFTQTIKTQKHYLDSLLHESGAVLFR 67

Query: 68  GFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQVP 127
           GFPV +A DFNDVVEAFG++E PYVGGAAPRT VVGRVFTANESPPDQKIPFHHE+AQV 
Sbjct: 68  GFPVNSADDFNDVVEAFGFDELPYVGGAAPRTSVVGRVFTANESPPDQKIPFHHEMAQVR 127

Query: 128 EFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFV-------------------- 187
           EFP+KLFFYCE++P+ GGETPIVLSH+VYER+K+K+PEFV                    
Sbjct: 128 EFPSKLFFYCEIEPKCGGETPIVLSHVVYERMKDKHPEFVQRLEEHGLLYVRVLGEDDDP 187

Query: 188 ---------------------ERAAKLGMKLEWLKD-GVKTVMGPIPAIKYDEVRQRKIW 247
                                +RA  LGMKLEW +D G KTVMGPIPAIKYDE R RK+W
Sbjct: 188 SSPIGRGWKSTFLTHDKNLAEQRAVDLGMKLEWTEDGGAKTVMGPIPAIKYDESRNRKVW 247

Query: 248 FNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLID 284
           FNSMVAAYTGWED RNDP KAVTFGDG PLPADI+H CLRILEEE VA+PWQ+GDVLLID
Sbjct: 248 FNSMVAAYTGWEDKRNDPRKAVTFGDGKPLPADIVHDCLRILEEECVAVPWQRGDVLLID 307

[Close]

BLAST of Lsi02G029840 vs. ExPASy Swiss-Prot
Match: E2JA28 (Dapdiamide synthesis protein DdaC OS=Enterobacter agglomerans OX=549 GN=ddaC PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 2.4e-20
Identity = 74/276 (26.81%), Postives = 108/276 (39.13%), Query Frame = 0


Query: 49  IDSLILKSGAILFRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPP 108
           ID ++ + GA+LFRGF +     F+ +V     EE  Y   +  R K    V+T+ E P 
Sbjct: 38  IDEILSEKGALLFRGFSINEDQQFSQLVSILAKEELTYQERSTQRKKTAQGVYTSTEYPA 97

Query: 109 DQKIPFHHELAQVPEFPAKLFFYCEVKPRSGGETPI-----VLSHLVYERV--------- 168
            + I  H E A     P K+ FY       GGETPI     VLS +  E V         
Sbjct: 98  AKTIANHSENAFQQVVPGKILFYAHQAALKGGETPIADNSRVLSLIDEEIVAEFRQKGIR 157

Query: 169 ------------------KEKYPEFVERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQ 228
                              EK  E      K  +  EWL D          A +   + +
Sbjct: 158 YLRNFDGGFDLSWQEAFQTEKKREVETYCVKNAIDCEWLSDSHLRTSQLRSATRRHPLNR 217

Query: 229 RKIWFNSM-VAAYTGWEDARNDPIKA----------VTFGDGTPLPADIIHGCLRILEEE 282
           +++WFN + +   T  E      + A            +G G  +P +++      L + 
Sbjct: 218 KEMWFNQLHLFHITNLELPVRQALLASLGHDLLPRHAVYGTGEEIPDEVVDHIRAALVKA 277

[Close]

BLAST of Lsi02G029840 vs. ExPASy TrEMBL
Match: A0A5A7V0W2 (Clavaminate synthase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00420 PE=4 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 9.8e-150
Identity = 267/324 (82.41%), Postives = 276/324 (85.19%), Query Frame = 0


Query: 1   MAVENFKEIQIPHQKHYHGSYFPLVLSPSSASFSISSFTRAIKAQKPIIDSLILKSGAIL 60
           MAVEN  EIQIPHQKHY GSYFPLVLSPSSASFSISSF +AIKAQKP IDSLIL SGAIL
Sbjct: 1   MAVENLMEIQIPHQKHYRGSYFPLVLSPSSASFSISSFAQAIKAQKPTIDSLILNSGAIL 60

Query: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQ 120
           FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPR+KVVGRVFTANESPPDQKIPFHHELAQ
Sbjct: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRSKVVGRVFTANESPPDQKIPFHHELAQ 120

Query: 121 VPEFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFV------------------ 180
           V EFPAKLFFYCEV+PRSGGETPIVLSH+VYERVKEKYPEFV                  
Sbjct: 121 VSEFPAKLFFYCEVEPRSGGETPIVLSHVVYERVKEKYPEFVERLEEQGLIYTRVLGEDD 180

Query: 181 -----------------------ERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKI 240
                                  ERAAKLGMKLEWLKDGVKTVMGPIPAIKYD+VRQRKI
Sbjct: 181 DPSSPIGRGWKSTFLTHDKSIAEERAAKLGMKLEWLKDGVKTVMGPIPAIKYDKVRQRKI 240

Query: 241 WFNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLI 284
           WFNSMVAAYTGWEDARNDP+KAVTFGDGTPLPADI++GCLRILEEESVAIPWQKGD+LLI
Sbjct: 241 WFNSMVAAYTGWEDARNDPVKAVTFGDGTPLPADIVYGCLRILEEESVAIPWQKGDILLI 300

[Close]

BLAST of Lsi02G029840 vs. ExPASy TrEMBL
Match: A0A1S3CGV9 (clavaminate synthase-like protein At3g21360 OS=Cucumis melo OX=3656 GN=LOC103500776 PE=4 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 9.8e-150
Identity = 267/324 (82.41%), Postives = 276/324 (85.19%), Query Frame = 0


Query: 1   MAVENFKEIQIPHQKHYHGSYFPLVLSPSSASFSISSFTRAIKAQKPIIDSLILKSGAIL 60
           MAVEN  EIQIPHQKHY GSYFPLVLSPSSASFSISSF +AIKAQKP IDSLIL SGAIL
Sbjct: 1   MAVENLMEIQIPHQKHYRGSYFPLVLSPSSASFSISSFAQAIKAQKPTIDSLILNSGAIL 60

Query: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQ 120
           FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPR+KVVGRVFTANESPPDQKIPFHHELAQ
Sbjct: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRSKVVGRVFTANESPPDQKIPFHHELAQ 120

Query: 121 VPEFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFV------------------ 180
           V EFPAKLFFYCEV+PRSGGETPIVLSH+VYERVKEKYPEFV                  
Sbjct: 121 VSEFPAKLFFYCEVEPRSGGETPIVLSHVVYERVKEKYPEFVERLEEQGLIYTRVLGEDD 180

Query: 181 -----------------------ERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKI 240
                                  ERAAKLGMKLEWLKDGVKTVMGPIPAIKYD+VRQRKI
Sbjct: 181 DPSSPIGRGWKSTFLTHDKSIAEERAAKLGMKLEWLKDGVKTVMGPIPAIKYDKVRQRKI 240

Query: 241 WFNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLI 284
           WFNSMVAAYTGWEDARNDP+KAVTFGDGTPLPADI++GCLRILEEESVAIPWQKGD+LLI
Sbjct: 241 WFNSMVAAYTGWEDARNDPVKAVTFGDGTPLPADIVYGCLRILEEESVAIPWQKGDILLI 300

[Close]

BLAST of Lsi02G029840 vs. ExPASy TrEMBL
Match: A0A6J1HN97 (clavaminate synthase-like protein At3g21360 OS=Cucurbita moschata OX=3662 GN=LOC111464478 PE=4 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.1e-148
Identity = 261/324 (80.56%), Postives = 278/324 (85.80%), Query Frame = 0


Query: 1   MAVENFKEIQIPHQKHYHGSYFPLVLSPSSASFSISSFTRAIKAQKPIIDSLILKSGAIL 60
           MAV+NF EIQIPHQKHYHGSYFPLVLSPSSASF+ISSFTRAI+AQKP++DSLILKSGA+L
Sbjct: 1   MAVDNFMEIQIPHQKHYHGSYFPLVLSPSSASFTISSFTRAIEAQKPLLDSLILKSGAVL 60

Query: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQ 120
           FRGFPV+AASDFNDVVE+FG+EEFPYVGGAAPRT+VVGRVFTANESPPDQKIPFHHELAQ
Sbjct: 61  FRGFPVDAASDFNDVVESFGFEEFPYVGGAAPRTRVVGRVFTANESPPDQKIPFHHELAQ 120

Query: 121 VPEFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFV------------------ 180
           VPEFPAKLFFYCEV+P SGGETPIVLSH+VYE+VK KYPEFV                  
Sbjct: 121 VPEFPAKLFFYCEVEPTSGGETPIVLSHVVYEKVKVKYPEFVQRLEEQGLIYTRILGEDD 180

Query: 181 -----------------------ERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKI 240
                                  ERAAKLGMK+EWL DGVKTVMGPIPAIKYDEVRQRKI
Sbjct: 181 DPSSPIGRGWKSTFLTDDKRIAEERAAKLGMKVEWLNDGVKTVMGPIPAIKYDEVRQRKI 240

Query: 241 WFNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLI 284
           WFNSMVAAYTGWEDARNDP+KAVTFGDGTPLPADII+ CLRILEEESVAIPWQKGDVLLI
Sbjct: 241 WFNSMVAAYTGWEDARNDPVKAVTFGDGTPLPADIIYDCLRILEEESVAIPWQKGDVLLI 300

[Close]

BLAST of Lsi02G029840 vs. ExPASy TrEMBL
Match: A0A6J1KDG9 (clavaminate synthase-like protein At3g21360 OS=Cucurbita maxima OX=3661 GN=LOC111494627 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.2e-148
Identity = 261/324 (80.56%), Postives = 276/324 (85.19%), Query Frame = 0


Query: 1   MAVENFKEIQIPHQKHYHGSYFPLVLSPSSASFSISSFTRAIKAQKPIIDSLILKSGAIL 60
           MAV+NF EIQIPHQKHYHGSYFPLVLSPSSASF+ISSFTRAIKAQKP++DSLILKSGA+L
Sbjct: 1   MAVDNFMEIQIPHQKHYHGSYFPLVLSPSSASFTISSFTRAIKAQKPLLDSLILKSGAVL 60

Query: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQ 120
           FRGFPV+AASDFNDVVE+FG+EEFPYVGGAAPRT+VVGRVFTANESPPDQKIPFHHELAQ
Sbjct: 61  FRGFPVDAASDFNDVVESFGFEEFPYVGGAAPRTRVVGRVFTANESPPDQKIPFHHELAQ 120

Query: 121 VPEFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFV------------------ 180
           VPEFPAKLFFYCEV+P SGGETPIVLSH+VYE+VK  YPEFV                  
Sbjct: 121 VPEFPAKLFFYCEVEPISGGETPIVLSHVVYEKVKAIYPEFVQRLEEQGLIYTRILGEDD 180

Query: 181 -----------------------ERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKI 240
                                  ERAAKLGMK+EWL DGVKTVMGPIPAIKYDEVRQ KI
Sbjct: 181 DPSSPIGRGWKSTFLTDDKRIAEERAAKLGMKVEWLNDGVKTVMGPIPAIKYDEVRQHKI 240

Query: 241 WFNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLI 284
           WFNSMVAAYTGWEDARNDP+KAVTFGDGTPLPADIIH CLRILEEESVAIPWQKGDVLLI
Sbjct: 241 WFNSMVAAYTGWEDARNDPVKAVTFGDGTPLPADIIHDCLRILEEESVAIPWQKGDVLLI 300

[Close]

BLAST of Lsi02G029840 vs. ExPASy TrEMBL
Match: A0A0A0K9R6 (TauD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G452340 PE=4 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 7.1e-148
Identity = 264/324 (81.48%), Postives = 275/324 (84.88%), Query Frame = 0


Query: 1   MAVENFKEIQIPHQKHYHGSYFPLVLSPSSASFSISSFTRAIKAQKPIIDSLILKSGAIL 60
           MAVEN  EIQIPHQKHY  SYFPLVLSPSSASF++SSF +AIKAQK  IDSLIL SGAIL
Sbjct: 1   MAVENLMEIQIPHQKHYPASYFPLVLSPSSASFAVSSFAQAIKAQKSTIDSLILNSGAIL 60

Query: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQ 120
           FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPR+KVVGRVFTANESPPDQKIPFHHELAQ
Sbjct: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRSKVVGRVFTANESPPDQKIPFHHELAQ 120

Query: 121 VPEFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFV------------------ 180
           VPEFPAKLFFYCEV+PRSGGETPIVLSH+VYERVKEKYPEFV                  
Sbjct: 121 VPEFPAKLFFYCEVEPRSGGETPIVLSHVVYERVKEKYPEFVERLEEQGLIYTRVLGEDD 180

Query: 181 -----------------------ERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKI 240
                                  ERAAKLGMKLEWLKDGVKTVMGPIPAIK+D+VRQRKI
Sbjct: 181 DPSSPIGRGWKSTFLTHDKSIAEERAAKLGMKLEWLKDGVKTVMGPIPAIKHDKVRQRKI 240

Query: 241 WFNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLI 284
           WFNSMVAAYTGWEDARNDP+KAVTFGDGTPLPADII+GCLRILEEESVAIPWQKGD+LLI
Sbjct: 241 WFNSMVAAYTGWEDARNDPVKAVTFGDGTPLPADIIYGCLRILEEESVAIPWQKGDILLI 300

[Close]

BLAST of Lsi02G029840 vs. NCBI nr
Match: XP_038899975.1 (clavaminate synthase-like protein At3g21360 [Benincasa hispida])

HSP 1 Score: 548.9 bits (1413), Expect = 2.6e-152
Identity = 270/324 (83.33%), Postives = 280/324 (86.42%), Query Frame = 0


Query: 1   MAVENFKEIQIPHQKHYHGSYFPLVLSPSSASFSISSFTRAIKAQKPIIDSLILKSGAIL 60
           MA+ENF EIQIPHQKHYHGS+FPLVLSPSSASFSISSFTRAIKAQKP+IDSLILKSGA+L
Sbjct: 1   MAIENFVEIQIPHQKHYHGSFFPLVLSPSSASFSISSFTRAIKAQKPVIDSLILKSGAVL 60

Query: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQ 120
           FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQ
Sbjct: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQ 120

Query: 121 VPEFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFV------------------ 180
           VPEFPAKLFFYCEV+  SGGETPIVLSH+VYERV+EKYPEFV                  
Sbjct: 121 VPEFPAKLFFYCEVEASSGGETPIVLSHVVYERVREKYPEFVERLEEQGLIYTRVLGEGD 180

Query: 181 -----------------------ERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKI 240
                                  ERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKI
Sbjct: 181 DPSSPIGRGWKSTFLTDDKGIAEERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKI 240

Query: 241 WFNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLI 284
           WFNSMVAAYTGWEDARNDP+KAVTFGDGTPLPADII+GCLRILEEESVAIPWQKGD+LLI
Sbjct: 241 WFNSMVAAYTGWEDARNDPVKAVTFGDGTPLPADIIYGCLRILEEESVAIPWQKGDILLI 300

[Close]

BLAST of Lsi02G029840 vs. NCBI nr
Match: XP_008462414.1 (PREDICTED: clavaminate synthase-like protein At3g21360 [Cucumis melo] >KAA0059475.1 clavaminate synthase-like protein [Cucumis melo var. makuwa] >TYK03848.1 clavaminate synthase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 539.3 bits (1388), Expect = 2.0e-149
Identity = 267/324 (82.41%), Postives = 276/324 (85.19%), Query Frame = 0


Query: 1   MAVENFKEIQIPHQKHYHGSYFPLVLSPSSASFSISSFTRAIKAQKPIIDSLILKSGAIL 60
           MAVEN  EIQIPHQKHY GSYFPLVLSPSSASFSISSF +AIKAQKP IDSLIL SGAIL
Sbjct: 1   MAVENLMEIQIPHQKHYRGSYFPLVLSPSSASFSISSFAQAIKAQKPTIDSLILNSGAIL 60

Query: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQ 120
           FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPR+KVVGRVFTANESPPDQKIPFHHELAQ
Sbjct: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRSKVVGRVFTANESPPDQKIPFHHELAQ 120

Query: 121 VPEFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFV------------------ 180
           V EFPAKLFFYCEV+PRSGGETPIVLSH+VYERVKEKYPEFV                  
Sbjct: 121 VSEFPAKLFFYCEVEPRSGGETPIVLSHVVYERVKEKYPEFVERLEEQGLIYTRVLGEDD 180

Query: 181 -----------------------ERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKI 240
                                  ERAAKLGMKLEWLKDGVKTVMGPIPAIKYD+VRQRKI
Sbjct: 181 DPSSPIGRGWKSTFLTHDKSIAEERAAKLGMKLEWLKDGVKTVMGPIPAIKYDKVRQRKI 240

Query: 241 WFNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLI 284
           WFNSMVAAYTGWEDARNDP+KAVTFGDGTPLPADI++GCLRILEEESVAIPWQKGD+LLI
Sbjct: 241 WFNSMVAAYTGWEDARNDPVKAVTFGDGTPLPADIVYGCLRILEEESVAIPWQKGDILLI 300

[Close]

BLAST of Lsi02G029840 vs. NCBI nr
Match: XP_022964464.1 (clavaminate synthase-like protein At3g21360 [Cucurbita moschata])

HSP 1 Score: 535.8 bits (1379), Expect = 2.2e-148
Identity = 261/324 (80.56%), Postives = 278/324 (85.80%), Query Frame = 0


Query: 1   MAVENFKEIQIPHQKHYHGSYFPLVLSPSSASFSISSFTRAIKAQKPIIDSLILKSGAIL 60
           MAV+NF EIQIPHQKHYHGSYFPLVLSPSSASF+ISSFTRAI+AQKP++DSLILKSGA+L
Sbjct: 1   MAVDNFMEIQIPHQKHYHGSYFPLVLSPSSASFTISSFTRAIEAQKPLLDSLILKSGAVL 60

Query: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQ 120
           FRGFPV+AASDFNDVVE+FG+EEFPYVGGAAPRT+VVGRVFTANESPPDQKIPFHHELAQ
Sbjct: 61  FRGFPVDAASDFNDVVESFGFEEFPYVGGAAPRTRVVGRVFTANESPPDQKIPFHHELAQ 120

Query: 121 VPEFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFV------------------ 180
           VPEFPAKLFFYCEV+P SGGETPIVLSH+VYE+VK KYPEFV                  
Sbjct: 121 VPEFPAKLFFYCEVEPTSGGETPIVLSHVVYEKVKVKYPEFVQRLEEQGLIYTRILGEDD 180

Query: 181 -----------------------ERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKI 240
                                  ERAAKLGMK+EWL DGVKTVMGPIPAIKYDEVRQRKI
Sbjct: 181 DPSSPIGRGWKSTFLTDDKRIAEERAAKLGMKVEWLNDGVKTVMGPIPAIKYDEVRQRKI 240

Query: 241 WFNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLI 284
           WFNSMVAAYTGWEDARNDP+KAVTFGDGTPLPADII+ CLRILEEESVAIPWQKGDVLLI
Sbjct: 241 WFNSMVAAYTGWEDARNDPVKAVTFGDGTPLPADIIYDCLRILEEESVAIPWQKGDVLLI 300

[Close]

BLAST of Lsi02G029840 vs. NCBI nr
Match: XP_023515356.1 (clavaminate synthase-like protein At3g21360 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535.0 bits (1377), Expect = 3.8e-148
Identity = 260/324 (80.25%), Postives = 278/324 (85.80%), Query Frame = 0


Query: 1   MAVENFKEIQIPHQKHYHGSYFPLVLSPSSASFSISSFTRAIKAQKPIIDSLILKSGAIL 60
           MAV+NF EIQIPHQKHYHGSYFPLVLSPSSASF+ISSFTRAIKAQKP++DSLILKSGA+L
Sbjct: 1   MAVDNFMEIQIPHQKHYHGSYFPLVLSPSSASFTISSFTRAIKAQKPLLDSLILKSGAVL 60

Query: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQ 120
           FRGFPV+++SDFNDVVE+FG+EEFPYVGGAAPRT+VVGRVFTANESPPDQKIPFHHELAQ
Sbjct: 61  FRGFPVDSSSDFNDVVESFGFEEFPYVGGAAPRTRVVGRVFTANESPPDQKIPFHHELAQ 120

Query: 121 VPEFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFV------------------ 180
           VPEFPAKLFFYCEV+P SGGETPIVLSH+VYE+VK KYPEFV                  
Sbjct: 121 VPEFPAKLFFYCEVEPTSGGETPIVLSHVVYEKVKVKYPEFVQRLEEQGLIYTRILGEDD 180

Query: 181 -----------------------ERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKI 240
                                  ERAAKLGMK+EWL DGVKTVMGPIPAIKYDEVRQRKI
Sbjct: 181 DPSSPIGRGWKSTFLTDDKRIAEERAAKLGMKVEWLNDGVKTVMGPIPAIKYDEVRQRKI 240

Query: 241 WFNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLI 284
           WFNSMVAAYTGWEDARNDP+KAVTFGDGTPLPADII+ CLRILEEESVAIPWQKGDVLLI
Sbjct: 241 WFNSMVAAYTGWEDARNDPVKAVTFGDGTPLPADIIYDCLRILEEESVAIPWQKGDVLLI 300

[Close]

BLAST of Lsi02G029840 vs. NCBI nr
Match: KAG7025838.1 (Clavaminate synthase-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 534.6 bits (1376), Expect = 5.0e-148
Identity = 261/324 (80.56%), Postives = 277/324 (85.49%), Query Frame = 0


Query: 1   MAVENFKEIQIPHQKHYHGSYFPLVLSPSSASFSISSFTRAIKAQKPIIDSLILKSGAIL 60
           MAV+NF EIQIPHQKHYHGSYFPLVLSPSSASF+ SSFTRAIKAQKP++DSLILKSGA+L
Sbjct: 1   MAVDNFMEIQIPHQKHYHGSYFPLVLSPSSASFTNSSFTRAIKAQKPLLDSLILKSGAVL 60

Query: 61  FRGFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQ 120
           FRGFPV+AASDFNDVVE+FG+EEFPYVGGAAPRT+VVGRVFTANESPPDQKIPFHHELAQ
Sbjct: 61  FRGFPVDAASDFNDVVESFGFEEFPYVGGAAPRTRVVGRVFTANESPPDQKIPFHHELAQ 120

Query: 121 VPEFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFV------------------ 180
           VPEFPAKLFFYCEV+P SGGETPIVLSH+VYE+VK KYPEFV                  
Sbjct: 121 VPEFPAKLFFYCEVEPTSGGETPIVLSHVVYEKVKVKYPEFVQRLEEQGLIYTRILGEDD 180

Query: 181 -----------------------ERAAKLGMKLEWLKDGVKTVMGPIPAIKYDEVRQRKI 240
                                  ERAAKLGMK+EWL DGVKTVMGPIPAIKYDEVRQRKI
Sbjct: 181 DPSSPIGRGWKSTFLTDDKRIAEERAAKLGMKVEWLNDGVKTVMGPIPAIKYDEVRQRKI 240

Query: 241 WFNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLI 284
           WFNSMVAAYTGWEDARNDP+KAVTFGDGTPLPADII+ CLRILEEESVAIPWQKGDVLLI
Sbjct: 241 WFNSMVAAYTGWEDARNDPVKAVTFGDGTPLPADIIYDCLRILEEESVAIPWQKGDVLLI 300

[Close]

BLAST of Lsi02G029840 vs. TAIR 10
Match: AT3G21360.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 435.3 bits (1118), Expect = 3.9e-122
Identity = 214/323 (66.25%), Postives = 241/323 (74.61%), Query Frame = 0


Query: 8   EIQIPHQKHYHGSYFPLVLSPSSAS-----FSISSFTRAIKAQKPIIDSLILKSGAILFR 67
           E  IP QKHY    FP V+SP SAS      S+  FT+ IK QK  +DSL+ +SGA+LFR
Sbjct: 8   ETPIPQQKHYESKPFPAVISPPSASIPIPALSLPLFTQTIKTQKHYLDSLLHESGAVLFR 67

Query: 68  GFPVEAASDFNDVVEAFGYEEFPYVGGAAPRTKVVGRVFTANESPPDQKIPFHHELAQVP 127
           GFPV +A DFNDVVEAFG++E PYVGGAAPRT VVGRVFTANESPPDQKIPFHHE+AQV 
Sbjct: 68  GFPVNSADDFNDVVEAFGFDELPYVGGAAPRTSVVGRVFTANESPPDQKIPFHHEMAQVR 127

Query: 128 EFPAKLFFYCEVKPRSGGETPIVLSHLVYERVKEKYPEFV-------------------- 187
           EFP+KLFFYCE++P+ GGETPIVLSH+VYER+K+K+PEFV                    
Sbjct: 128 EFPSKLFFYCEIEPKCGGETPIVLSHVVYERMKDKHPEFVQRLEEHGLLYVRVLGEDDDP 187

Query: 188 ---------------------ERAAKLGMKLEWLKD-GVKTVMGPIPAIKYDEVRQRKIW 247
                                +RA  LGMKLEW +D G KTVMGPIPAIKYDE R RK+W
Sbjct: 188 SSPIGRGWKSTFLTHDKNLAEQRAVDLGMKLEWTEDGGAKTVMGPIPAIKYDESRNRKVW 247

Query: 248 FNSMVAAYTGWEDARNDPIKAVTFGDGTPLPADIIHGCLRILEEESVAIPWQKGDVLLID 284
           FNSMVAAYTGWED RNDP KAVTFGDG PLPADI+H CLRILEEE VA+PWQ+GDVLLID
Sbjct: 248 FNSMVAAYTGWEDKRNDPRKAVTFGDGKPLPADIVHDCLRILEEECVAVPWQRGDVLLID 307

The following BLAST results are available for this feature:

BLAST of Lsi02G029840 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LIG0	5.4e-121	66.25	Clavaminate synthase-like protein At3g21360 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
E2JA28	2.4e-20	26.81	Dapdiamide synthesis protein DdaC OS=Enterobacter agglomerans OX=549 GN=ddaC PE=...	[more]

BLAST of Lsi02G029840 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7V0W2	9.8e-150	82.41	Clavaminate synthase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3CGV9	9.8e-150	82.41	clavaminate synthase-like protein At3g21360 OS=Cucumis melo OX=3656 GN=LOC103500...	[more]
A0A6J1HN97	1.1e-148	80.56	clavaminate synthase-like protein At3g21360 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1KDG9	3.2e-148	80.56	clavaminate synthase-like protein At3g21360 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A0A0K9R6	7.1e-148	81.48	TauD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G452340 PE=4 S...	[more]

BLAST of Lsi02G029840 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899975.1	2.6e-152	83.33	clavaminate synthase-like protein At3g21360 [Benincasa hispida]	[more]
XP_008462414.1	2.0e-149	82.41	PREDICTED: clavaminate synthase-like protein At3g21360 [Cucumis melo] >KAA005947...	[more]
XP_022964464.1	2.2e-148	80.56	clavaminate synthase-like protein At3g21360 [Cucurbita moschata]	[more]
XP_023515356.1	3.8e-148	80.25	clavaminate synthase-like protein At3g21360 [Cucurbita pepo subsp. pepo]	[more]
KAG7025838.1	5.0e-148	80.56	Clavaminate synthase-like protein, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Lsi02G029840 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G21360.1	3.9e-122	66.25	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003819	TauD/TfdA-like domain	PFAM	PF02668	TauD	coord: 31..278 e-value: 1.3E-35 score: 123.5
IPR042098	Taurine dioxygenase TauD-like superfamily	GENE3D	3.60.130.10		coord: 168..283 e-value: 9.9E-44 score: 151.6 coord: 1..167 e-value: 2.4E-54 score: 186.4
None	No IPR available	PANTHER	PTHR10696:SF43	TAUD/TFDA-LIKE DOMAIN-CONTAINING PROTEIN-RELATED	coord: 163..283 coord: 6..164
None	No IPR available	PANTHER	PTHR10696	GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED	coord: 163..283 coord: 6..164
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 12..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G029840.1	Lsi02G029840.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0016491	oxidoreductase activity