Lsi02G028990 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G028990
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description5'-phosphoribosylglycinamide transformylase
Locationchr02: 35230689 .. 35231576 (-)
RNA-Seq ExpressionLsi02G028990
SyntenyLsi02G028990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCTCAATGTTCTCTCTATCGGATTTGCCCAAATCTGTCTGTTCCGTTGATCCGATCTCCCAATCATTCGTTCCTTTTGAAGCTTCATTCTCCTCATTATTCTCGCTCCATTGTTCGAACCAATCATTCCACTTCGCATAGTGTTTGTTTCAGGGAGAATATTGCTTGTAGAAAGAACAGTCAAATGATTGTGAATGCCAGTCTCGAAGGGGAAGTTTCAAGAAGTGTGATTAGGAAGAAGAAACTTGCTGTTTTTGTTTCTGGTGGAGGCTCCAATTTTCGTTCAATACACGAGGCATCTCTTGAAGGGTTAATTTATGGAGAAACTGTTGTAGTGGTTACTAACAGAAGTGGTTGTGGTGGTGCAGAATATGCAAGAACTAAGAACATTCCAGTTATTTTGTTTCCTAAAACAAAAGATGAACCCCATGGCTTATCTGCATCTGAGCTTGTAGCTACCCTTAGAAATTTTGAGATTGATTTCATTCTTCTTGCTGGATTCATGAAACTTATACCCCTGGAATTGATACAAGCTTATTCCAAATCTATAGTAAATATACACCCATCACTTCTTCCTGCTTTTGGAGGCAAAGGGTACTATGGAATGAAGGTGCACAAGGCAGTGATTGACTCTGGAGCTCGATTTTCGGGTCCAACAATTCATTTGGTTGATGAGCACTTCGACACTGGACGGATACTTGCTCAAAGAGTGGTTCCTGTGCTTGCAAATGATTCAGCAGAGGATCTAGCAGCTAGGGTTCTTAAGGAGGAGCATCGTCTTTATGTCGAGGTAGTTGCAGCATTGTGTGACGAACGGATCGTTTGGAGAGCAGATGGTGTTCCTCTCATTCGAAGCAAAGAAAATCCTAATGAATTATACTAG

mRNA sequence

ATGGAAGCTCAATGTTCTCTCTATCGGATTTGCCCAAATCTGTCTGTTCCGTTGATCCGATCTCCCAATCATTCGTTCCTTTTGAAGCTTCATTCTCCTCATTATTCTCGCTCCATTGTTCGAACCAATCATTCCACTTCGCATAGTGTTTGTTTCAGGGAGAATATTGCTTGTAGAAAGAACAGTCAAATGATTGTGAATGCCAGTCTCGAAGGGGAAGTTTCAAGAAGTGTGATTAGGAAGAAGAAACTTGCTGTTTTTGTTTCTGGTGGAGGCTCCAATTTTCGTTCAATACACGAGGCATCTCTTGAAGGGTTAATTTATGGAGAAACTGTTGTAGTGGTTACTAACAGAAGTGGTTGTGGTGGTGCAGAATATGCAAGAACTAAGAACATTCCAGTTATTTTGTTTCCTAAAACAAAAGATGAACCCCATGGCTTATCTGCATCTGAGCTTGTAGCTACCCTTAGAAATTTTGAGATTGATTTCATTCTTCTTGCTGGATTCATGAAACTTATACCCCTGGAATTGATACAAGCTTATTCCAAATCTATAGTAAATATACACCCATCACTTCTTCCTGCTTTTGGAGGCAAAGGGTACTATGGAATGAAGGTGCACAAGGCAGTGATTGACTCTGGAGCTCGATTTTCGGGTCCAACAATTCATTTGGTTGATGAGCACTTCGACACTGGACGGATACTTGCTCAAAGAGTGGTTCCTGTGCTTGCAAATGATTCAGCAGAGGATCTAGCAGCTAGGGTTCTTAAGGAGGAGCATCGTCTTTATGTCGAGGTAGTTGCAGCATTGTGTGACGAACGGATCGTTTGGAGAGCAGATGGTGTTCCTCTCATTCGAAGCAAAGAAAATCCTAATGAATTATACTAG

Coding sequence (CDS)

ATGGAAGCTCAATGTTCTCTCTATCGGATTTGCCCAAATCTGTCTGTTCCGTTGATCCGATCTCCCAATCATTCGTTCCTTTTGAAGCTTCATTCTCCTCATTATTCTCGCTCCATTGTTCGAACCAATCATTCCACTTCGCATAGTGTTTGTTTCAGGGAGAATATTGCTTGTAGAAAGAACAGTCAAATGATTGTGAATGCCAGTCTCGAAGGGGAAGTTTCAAGAAGTGTGATTAGGAAGAAGAAACTTGCTGTTTTTGTTTCTGGTGGAGGCTCCAATTTTCGTTCAATACACGAGGCATCTCTTGAAGGGTTAATTTATGGAGAAACTGTTGTAGTGGTTACTAACAGAAGTGGTTGTGGTGGTGCAGAATATGCAAGAACTAAGAACATTCCAGTTATTTTGTTTCCTAAAACAAAAGATGAACCCCATGGCTTATCTGCATCTGAGCTTGTAGCTACCCTTAGAAATTTTGAGATTGATTTCATTCTTCTTGCTGGATTCATGAAACTTATACCCCTGGAATTGATACAAGCTTATTCCAAATCTATAGTAAATATACACCCATCACTTCTTCCTGCTTTTGGAGGCAAAGGGTACTATGGAATGAAGGTGCACAAGGCAGTGATTGACTCTGGAGCTCGATTTTCGGGTCCAACAATTCATTTGGTTGATGAGCACTTCGACACTGGACGGATACTTGCTCAAAGAGTGGTTCCTGTGCTTGCAAATGATTCAGCAGAGGATCTAGCAGCTAGGGTTCTTAAGGAGGAGCATCGTCTTTATGTCGAGGTAGTTGCAGCATTGTGTGACGAACGGATCGTTTGGAGAGCAGATGGTGTTCCTCTCATTCGAAGCAAAGAAAATCCTAATGAATTATACTAG

Protein sequence

MEAQCSLYRICPNLSVPLIRSPNHSFLLKLHSPHYSRSIVRTNHSTSHSVCFRENIACRKNSQMIVNASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSGCGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLELIQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVPLIRSKENPNELY
Homology
BLAST of Lsi02G028990 vs. ExPASy Swiss-Prot
Match: P52423 (Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Vigna unguiculata OX=3917 GN=PUR3 PE=2 SV=2)

HSP 1 Score: 364.0 bits (933), Expect = 1.6e-99
Identity = 192/310 (61.94%), Postives = 227/310 (73.23%), Query Frame = 0

Query: 1   MEAQCSLYRICPNL----SVPLIRSPNHSFLLKLHS----PHYSRSIVRTNHSTSHSVCF 60
           MEAQ  + R CP      S+P+++ P       LHS    P      +       H++ F
Sbjct: 1   MEAQQIISRFCPKSSLAPSIPMVKQPFSLNFPPLHSLSSYPFLQSQNLGFPTGALHAISF 60

Query: 61  RE--------NIACRKNSQMIVNASLEGEVSRSV-IRKKKLAVFVSGGGSNFRSIHEASL 120
                      I C K+S        + EV   V +R+KKLAVFVSGGGSNFRSIHEAS 
Sbjct: 61  VHKEVCSSSWRIWCSKSSSSTAEPEEDHEVRAQVTVRRKKLAVFVSGGGSNFRSIHEASK 120

Query: 121 EGLIYGETVVVVTNRSGCGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDF 180
           +G ++G+  V+VTN+S CGGA+YAR   IPVILFPK KDEP GLS  +LV TLR FE+DF
Sbjct: 121 KGSLHGDVTVLVTNKSECGGAQYARNNGIPVILFPKAKDEPKGLSPCDLVDTLRKFEVDF 180

Query: 181 ILLAGFMKLIPLELIQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIH 240
           +LLAG++KLIP+ELI+A+ +SI NIHPSLLPAFGGKGYYGMKVHKAVI SGARFSGPTIH
Sbjct: 181 VLLAGYLKLIPVELIRAFERSIFNIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIH 240

Query: 241 LVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVP 294
            VDEH+DTGRILAQRVVPVLAND+AE+LAARVL EEH+LYVEVV ALC+ERIVWR DGVP
Sbjct: 241 FVDEHYDTGRILAQRVVPVLANDTAEELAARVLNEEHQLYVEVVEALCEERIVWRKDGVP 300

BLAST of Lsi02G028990 vs. ExPASy Swiss-Prot
Match: P52422 (Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PUR3 PE=2 SV=2)

HSP 1 Score: 309.7 bits (792), Expect = 3.6e-83
Identity = 146/217 (67.28%), Postives = 182/217 (83.87%), Query Frame = 0

Query: 80  RKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSGCGGAEYARTKNIPVILFPK 139
           R+KKLAVFVSGGGSNFR IHE   +G + G+ V++VTN+  CGGAEYAR+  IPV++FPK
Sbjct: 76  RRKKLAVFVSGGGSNFRKIHEGCSDGSVNGDVVLLVTNKKDCGGAEYARSNGIPVLVFPK 135

Query: 140 TKDEP-HGLSASELVATLRNFEIDFILLAGFMKLIPLELIQAYSKSIVNIHPSLLPAFGG 199
            K EP  GLS SELV  LR + +DF+LLAG++KLIP+EL+QA+ K I+NIHP+LLPAFGG
Sbjct: 136 AKREPSDGLSPSELVDVLRKYGVDFVLLAGYLKLIPVELVQAFPKRILNIHPALLPAFGG 195

Query: 200 KGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKE 259
           KG YG+KVHKAV++SGAR+SGPTIH V+E +DTGRILAQ  V V+AND+ E+LA RVL E
Sbjct: 196 KGLYGIKVHKAVLESGARYSGPTIHFVNEEYDTGRILAQSAVRVIANDTPEELAKRVLHE 255

Query: 260 EHRLYVEVVAALCDERIVWRADGVPLIRSKENPNELY 296
           EH+LYVEVV A+C+ERI WR DGVPLI++K+NP+E Y
Sbjct: 256 EHKLYVEVVGAICEERIKWREDGVPLIQNKQNPDEYY 292

BLAST of Lsi02G028990 vs. ExPASy Swiss-Prot
Match: Q42805 (Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Glycine max OX=3847 GN=PUR3 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.6e-73
Identity = 150/245 (61.22%), Postives = 181/245 (73.88%), Query Frame = 0

Query: 54  ENIACRKNSQMIVNASLEGEVSRSV-IRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETV 113
           + I C  +S          EV   V +R+KKL VFVSGGG+NFR+IHEA+  G ++G+ +
Sbjct: 57  KRIWCSSSSSSTAEPKEGHEVRAQVTVRRKKLGVFVSGGGTNFRAIHEATKRGSLHGDVL 116

Query: 114 VVVTNRSGCGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKL 173
           V+VTN+S CGGAEYAR   IPVIL+  +KDE +G   S+LV TLR FE+DFILLAG+   
Sbjct: 117 VLVTNKSDCGGAEYARNNGIPVILYHISKDESNG---SDLVDTLRKFEVDFILLAGY--- 176

Query: 174 IPLELIQAYSKSIVNI---HPSLL-PAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEH 233
             L L Q    S+  I   H S+   +F  +G +GMKVHKAVI SGARFSGPTIH VDEH
Sbjct: 177 --LNLYQWNDPSLQKIYIQHSSITSSSFWRQGIHGMKVHKAVIASGARFSGPTIHFVDEH 236

Query: 234 FDTGRILAQRVVPVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVPLIRSK 293
           +DTGRILAQRVVPV AND+ E+LAARVLKEEH+LYVEVV ALC+ER+VWR DGVPLI+SK
Sbjct: 237 YDTGRILAQRVVPVQANDTVEELAARVLKEEHQLYVEVVEALCEERVVWRQDGVPLIQSK 293

BLAST of Lsi02G028990 vs. ExPASy Swiss-Prot
Match: P00967 (Trifunctional purine biosynthetic protein adenosine-3 OS=Drosophila melanogaster OX=7227 GN=Gart PE=1 SV=2)

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-27
Identity = 73/216 (33.80%), Postives = 118/216 (54.63%), Query Frame = 0

Query: 60   KNSQMIV---NASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGL--IYGETVVV 119
            K SQ++V    ASL         R+K++AV +SG GSN +++ +A+ +    I+ + V+V
Sbjct: 1129 KKSQVVVQNFEASLARTQKMLSQRRKRVAVLISGTGSNLQALIDATRDSAQGIHADVVLV 1188

Query: 120  VTNRSGCGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIP 179
            ++N+ G  G + A    IP ++          +  +EL   L+   +D I LAGFM+++ 
Sbjct: 1189 ISNKPGVLGLQRATQAGIPSLVISHKDFASREVYDAELTRNLKAARVDLICLAGFMRVLS 1248

Query: 180  LELIQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRI 239
               ++ +   +VNIHPSLLP      Y G+ V K  +++G + SG T+H VDE  DTG I
Sbjct: 1249 APFVREWRGRLVNIHPSLLPK-----YPGLHVQKQALEAGEKESGCTVHFVDEGVDTGAI 1308

Query: 240  LAQRVVPVLANDSAEDLAARVLKEEHRLYVEVVAAL 271
            + Q  VP+L +D  + L  R+ K EH  +   +A L
Sbjct: 1309 IVQAAVPILPDDDEDSLTQRIHKAEHWAFPRALAML 1339

BLAST of Lsi02G028990 vs. ExPASy Swiss-Prot
Match: P21872 (Trifunctional purine biosynthetic protein adenosine-3 OS=Gallus gallus OX=9031 GN=GART PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.8e-26
Identity = 67/207 (32.37%), Postives = 112/207 (54.11%), Query Frame = 0

Query: 57  ACRKNSQMIVNASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVT 116
           A + N  + V++ ++G++  +   K K+AV +SG G+N  ++  ++ +   + E V+VV+
Sbjct: 782 ALQANRSLSVHSHIQGKIQTN---KVKVAVLISGTGTNLEALINSTKKPTSFAEIVLVVS 841

Query: 117 NRSGCGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLE 176
           N++G  G   A    IP  +    +        S +   L  F ++ I LAGFM+++   
Sbjct: 842 NKAGVEGLRKAERAGIPTRVIDHKQYGSRTEFDSAVDRVLEEFSVELICLAGFMRILSGP 901

Query: 177 LIQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILA 236
            ++ +   I+NIHPSLLP+F      G   HK V+++G R +G T+H V E  D G I+ 
Sbjct: 902 FVKKWEGKILNIHPSLLPSF-----KGANAHKLVLEAGVRVTGCTVHFVAEEVDAGAIIF 961

Query: 237 QRVVPVLANDSAEDLAARVLKEEHRLY 264
           Q  VPV   D+ E L+ RV + EHR +
Sbjct: 962 QEAVPVKIGDTVETLSERVKEAEHRAF 980

BLAST of Lsi02G028990 vs. ExPASy TrEMBL
Match: A0A6J1JYY4 (phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488685 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 8.4e-136
Identity = 245/295 (83.05%), Postives = 270/295 (91.53%), Query Frame = 0

Query: 1   MEAQCSLYRICPNLSVPLIRSPNHSFLLKLHSPHYSRSIVRTNHSTSHSVCFRENIACRK 60
           MEAQCSL R CPNLS+PL+ SP H  LLK  S     S VRTNHSTS+SV F+ENIACRK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVLSPKHPILLKFPSYSSRPSFVRTNHSTSNSVWFKENIACRK 60

Query: 61  NSQMIVNASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSG 120
           N Q++ ++SLEG+VSR VIRKKKLAVFVSGGGSNFRSIHEAS+EG I+GE  VVVTN+SG
Sbjct: 61  NCQVVASSSLEGDVSRIVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120

Query: 121 CGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLELIQA 180
           CGGAEYAR+KNIPVILFPKTKDEPHGLSAS+LVATLR+FEIDFILLAG++KLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVV 240
           YSKSI+NIHPSLLP+FGGKGYYGMKVHKAVI SGARFSGPTIHLVDEHFDTG+ILAQRVV
Sbjct: 181 YSKSIINIHPSLLPSFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVPLIRSKENPNELY 296
           PVLAND+AEDLAARVLKEEH LYVEVVAALCD+RIVWR DGVPLI+SK+NP+E Y
Sbjct: 241 PVLANDAAEDLAARVLKEEHHLYVEVVAALCDDRIVWREDGVPLIQSKDNPDEFY 295

BLAST of Lsi02G028990 vs. ExPASy TrEMBL
Match: A0A0A0K7R9 (Formyl_trans_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G450470 PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.1e-135
Identity = 248/295 (84.07%), Postives = 274/295 (92.88%), Query Frame = 0

Query: 1   MEAQCSLYRICPNLSVPLIRSPNHSFLLKLHSPHYSRSIVRTNHSTSHSVCFRENIACRK 60
           MEAQCSL R CP LS+ LIRSPN+SFLLKL  P  S S V+TNHST  SV F+ENIA RK
Sbjct: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKL--PSSSSSFVQTNHSTLRSVWFKENIARRK 60

Query: 61  NSQMIVNASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSG 120
           N QM+V+ASL+GEVS++VI+KKKLAVFVSGGGSNFRSIHEASLEG ++GE VVVVTN+SG
Sbjct: 61  NVQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSG 120

Query: 121 CGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLELIQA 180
           CGGAEYAR+KNIPV+LFPKTKDEP+GLSASELVATL++FEIDFILLAG++KLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLKHFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVV 240
           YSKSIVNIHPSLLPAFGGKGYYGMKVHKAV++SGARFSG TIHLVDEHFDTGRILAQRVV
Sbjct: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVPLIRSKENPNELY 296
           PVLANDSAEDLAARVLKEEH LY EVVAALCDERI+WR+DGVPLI+SKE+PNE Y
Sbjct: 241 PVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKEHPNEFY 293

BLAST of Lsi02G028990 vs. ExPASy TrEMBL
Match: A0A1S3CGP8 (phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500692 PE=3 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.4e-135
Identity = 249/298 (83.56%), Postives = 273/298 (91.61%), Query Frame = 0

Query: 1   MEAQCSLYRICPNLSVPLIRSPNHSFLLKLHSPHY---SRSIVRTNHSTSHSVCFRENIA 60
           MEAQCSL R CPN S PLIRSPN+SFLLKL S  +   S S V+TNHST  SV F+ENIA
Sbjct: 1   MEAQCSLCRSCPNFSFPLIRSPNYSFLLKLPSSSFFSSSSSFVQTNHSTLRSVWFKENIA 60

Query: 61  CRKNSQMIVNASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTN 120
           CRK    +V+ASLEGEVS++VI+KKKLAVFVSGGGSNFRSIHEASLEGL++GE VVVVTN
Sbjct: 61  CRK----VVSASLEGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGLVHGEIVVVVTN 120

Query: 121 RSGCGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLEL 180
           +S CGGAEYAR+KNIPV+LFPKTKDEPHGLSASELVATLR+FEIDFILLAG++KLIPLEL
Sbjct: 121 KSSCGGAEYARSKNIPVLLFPKTKDEPHGLSASELVATLRHFEIDFILLAGYLKLIPLEL 180

Query: 181 IQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQ 240
           IQAYSK I+NIHPSLLPAFGGKGYYGMKVHKAV++SGARFSG TIHLVDEHFDTGRILAQ
Sbjct: 181 IQAYSKCILNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQ 240

Query: 241 RVVPVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVPLIRSKENPNELY 296
           RVVPVL+NDSAEDLAARVLKEEHRLY EVVAALCDERI+WR+DGVPLI+SKENPNE Y
Sbjct: 241 RVVPVLSNDSAEDLAARVLKEEHRLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294

BLAST of Lsi02G028990 vs. ExPASy TrEMBL
Match: A0A6J1GQR1 (phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111456613 PE=3 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 1.9e-135
Identity = 245/295 (83.05%), Postives = 269/295 (91.19%), Query Frame = 0

Query: 1   MEAQCSLYRICPNLSVPLIRSPNHSFLLKLHSPHYSRSIVRTNHSTSHSVCFRENIACRK 60
           MEAQCSL R CPNLS+PL+RSP H  LLK  S     S VRTNHSTS SV F+ENIACRK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFPSYSSRSSFVRTNHSTSSSVWFKENIACRK 60

Query: 61  NSQMIVNASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSG 120
           N +++ +ASLEG+VSR+VIRKKKLAVFVSGGGSNFRSIHEAS+EG I+GE  VVVTN+S 
Sbjct: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSS 120

Query: 121 CGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLELIQA 180
           CGGAEYAR+KNIPVILFPKTKDEPHGLSAS+LVATLR+FEIDFILLAG++KLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVV 240
           YSKSI+NIHPSLLPAFGGKGYYGMKVHKAVI SGARFSGPTIHLVDE+FDTG+ILAQRVV
Sbjct: 181 YSKSIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDENFDTGKILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVPLIRSKENPNELY 296
           PVLAND+AEDLAARVLKEEHRLYVEVVAALCD+RIVWR DGVPLI+SK+ P E Y
Sbjct: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKPGEFY 295

BLAST of Lsi02G028990 vs. ExPASy TrEMBL
Match: A0A6J1DNE2 (phosphoribosylglycinamide formyltransferase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111022206 PE=3 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.4e-122
Identity = 231/293 (78.84%), Postives = 254/293 (86.69%), Query Frame = 0

Query: 1   MEAQCSLYRICPNLSVPLIRSPNHSFLLKLHSPHYSRSIVRTNHSTSHSVCFRENIACRK 60
           MEAQCS  R CPNLSV   RSP  SFLLKL S   S S  R++  TS SV  ++N+  +K
Sbjct: 1   MEAQCSFSRFCPNLSVSFFRSPKLSFLLKLPSAS-SCSFARSSVPTSPSVWLKKNVGVKK 60

Query: 61  NSQMIVNASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSG 120
           NSQM+V  SLEGEVSR+VIR+KKLAVFVSGGGSNFRSIHEASLEG I G+ VV+VTN+  
Sbjct: 61  NSQMVVRVSLEGEVSRTVIRRKKLAVFVSGGGSNFRSIHEASLEGSINGDIVVLVTNKID 120

Query: 121 CGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLELIQA 180
           CGGAEYAR+KNIPVILFPKTKDEP  LS S+LVA LR  EIDFILLAG++KLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPDRLSPSDLVANLRRLEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVV 240
           YSKSI+NIHPSLLPAFGGKGYYGMKVHK+VI SGAR+SGPTIH VDEHFDTGRILAQRVV
Sbjct: 181 YSKSILNIHPSLLPAFGGKGYYGMKVHKSVIASGARYSGPTIHFVDEHFDTGRILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVPLIRSKENPNE 294
           PVLAND+A+DLAARVLKEEH LYVEVVAALC+ERIVWR DGVPLI+SKENPNE
Sbjct: 241 PVLANDAAQDLAARVLKEEHCLYVEVVAALCEERIVWREDGVPLIQSKENPNE 292

BLAST of Lsi02G028990 vs. NCBI nr
Match: XP_038896301.1 (phosphoribosylglycinamide formyltransferase, chloroplastic [Benincasa hispida])

HSP 1 Score: 516.5 bits (1329), Expect = 1.5e-142
Identity = 259/295 (87.80%), Postives = 277/295 (93.90%), Query Frame = 0

Query: 1   MEAQCSLYRICPNLSVPLIRSPNHSFLLKLHSPHYSRSIVRTNHSTSHSVCFRENIACRK 60
           ME Q SLYR+CP  SV LIR PNHSFL KL SP YSRS V+T+HST HSVCFR+NI CRK
Sbjct: 1   MEVQYSLYRVCPTPSVSLIRFPNHSFLSKLPSPCYSRSFVQTHHSTLHSVCFRKNITCRK 60

Query: 61  NSQMIVNASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSG 120
           N QMIVNASLEGEVSR+VIRKKKLAVFVSGGGSNFRSI+EASLEG I+GE VVVVTN+SG
Sbjct: 61  NGQMIVNASLEGEVSRTVIRKKKLAVFVSGGGSNFRSINEASLEGSIHGEIVVVVTNKSG 120

Query: 121 CGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLELIQA 180
           CGGAEYAR+K+IPVILFPKTKDEPHGLSAS+LVATL +FEIDF+LLAG++KLIP ELIQA
Sbjct: 121 CGGAEYARSKSIPVILFPKTKDEPHGLSASDLVATLGSFEIDFVLLAGYLKLIPPELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVV 240
           YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSG TIHLVDEHFDTGRILAQRVV
Sbjct: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGATIHLVDEHFDTGRILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVPLIRSKENPNELY 296
           PVLANDSAEDLAAR+LKEEHRLYVEVVAALCDERI+WRADGVPLI+SKENPNELY
Sbjct: 241 PVLANDSAEDLAARILKEEHRLYVEVVAALCDERIIWRADGVPLIQSKENPNELY 295

BLAST of Lsi02G028990 vs. NCBI nr
Match: XP_023547957.1 (phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 494.2 bits (1271), Expect = 7.8e-136
Identity = 246/295 (83.39%), Postives = 269/295 (91.19%), Query Frame = 0

Query: 1   MEAQCSLYRICPNLSVPLIRSPNHSFLLKLHSPHYSRSIVRTNHSTSHSVCFRENIACRK 60
           MEAQCSL R CPNLS+PL+RSP H  LLK  S     S VRTNHSTS SV F+E IACRK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKCPSYSSRSSFVRTNHSTSSSVWFKETIACRK 60

Query: 61  NSQMIVNASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSG 120
           N +++ +ASLEG+VSR+VIRKKKLAVFVSGGGSNFRSIHEAS+EG I+GE  VVVTN+SG
Sbjct: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120

Query: 121 CGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLELIQA 180
           CGGAEYAR+KNIPVILFPKTKDEPHGLSAS+LVATLR+FEIDFILLAG++KLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVV 240
           YSKSI+NIHPSLLPAFGGKGYYGMKVHKAVI SGARFSGPTIHLVDEHFDTG+ILAQRVV
Sbjct: 181 YSKSIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVPLIRSKENPNELY 296
           PVLAND+AEDLAARVLKEEHRLYVEVVAALCD+RIVWR DGVPLI+SK+ P E Y
Sbjct: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKPGEFY 295

BLAST of Lsi02G028990 vs. NCBI nr
Match: XP_022992348.1 (phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 493.0 bits (1268), Expect = 1.7e-135
Identity = 245/295 (83.05%), Postives = 270/295 (91.53%), Query Frame = 0

Query: 1   MEAQCSLYRICPNLSVPLIRSPNHSFLLKLHSPHYSRSIVRTNHSTSHSVCFRENIACRK 60
           MEAQCSL R CPNLS+PL+ SP H  LLK  S     S VRTNHSTS+SV F+ENIACRK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVLSPKHPILLKFPSYSSRPSFVRTNHSTSNSVWFKENIACRK 60

Query: 61  NSQMIVNASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSG 120
           N Q++ ++SLEG+VSR VIRKKKLAVFVSGGGSNFRSIHEAS+EG I+GE  VVVTN+SG
Sbjct: 61  NCQVVASSSLEGDVSRIVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120

Query: 121 CGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLELIQA 180
           CGGAEYAR+KNIPVILFPKTKDEPHGLSAS+LVATLR+FEIDFILLAG++KLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVV 240
           YSKSI+NIHPSLLP+FGGKGYYGMKVHKAVI SGARFSGPTIHLVDEHFDTG+ILAQRVV
Sbjct: 181 YSKSIINIHPSLLPSFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVPLIRSKENPNELY 296
           PVLAND+AEDLAARVLKEEH LYVEVVAALCD+RIVWR DGVPLI+SK+NP+E Y
Sbjct: 241 PVLANDAAEDLAARVLKEEHHLYVEVVAALCDDRIVWREDGVPLIQSKDNPDEFY 295

BLAST of Lsi02G028990 vs. NCBI nr
Match: XP_004141701.1 (phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis sativus])

HSP 1 Score: 492.7 bits (1267), Expect = 2.3e-135
Identity = 248/295 (84.07%), Postives = 274/295 (92.88%), Query Frame = 0

Query: 1   MEAQCSLYRICPNLSVPLIRSPNHSFLLKLHSPHYSRSIVRTNHSTSHSVCFRENIACRK 60
           MEAQCSL R CP LS+ LIRSPN+SFLLKL  P  S S V+TNHST  SV F+ENIA RK
Sbjct: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKL--PSSSSSFVQTNHSTLRSVWFKENIARRK 60

Query: 61  NSQMIVNASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSG 120
           N QM+V+ASL+GEVS++VI+KKKLAVFVSGGGSNFRSIHEASLEG ++GE VVVVTN+SG
Sbjct: 61  NVQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSG 120

Query: 121 CGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLELIQA 180
           CGGAEYAR+KNIPV+LFPKTKDEP+GLSASELVATL++FEIDFILLAG++KLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLKHFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVV 240
           YSKSIVNIHPSLLPAFGGKGYYGMKVHKAV++SGARFSG TIHLVDEHFDTGRILAQRVV
Sbjct: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVPLIRSKENPNELY 296
           PVLANDSAEDLAARVLKEEH LY EVVAALCDERI+WR+DGVPLI+SKE+PNE Y
Sbjct: 241 PVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKEHPNEFY 293

BLAST of Lsi02G028990 vs. NCBI nr
Match: XP_008462305.1 (PREDICTED: phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis melo] >XP_008462306.1 PREDICTED: phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis melo] >XP_008462307.1 PREDICTED: phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis melo] >XP_008462308.1 PREDICTED: phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis melo])

HSP 1 Score: 492.3 bits (1266), Expect = 3.0e-135
Identity = 249/298 (83.56%), Postives = 273/298 (91.61%), Query Frame = 0

Query: 1   MEAQCSLYRICPNLSVPLIRSPNHSFLLKLHSPHY---SRSIVRTNHSTSHSVCFRENIA 60
           MEAQCSL R CPN S PLIRSPN+SFLLKL S  +   S S V+TNHST  SV F+ENIA
Sbjct: 1   MEAQCSLCRSCPNFSFPLIRSPNYSFLLKLPSSSFFSSSSSFVQTNHSTLRSVWFKENIA 60

Query: 61  CRKNSQMIVNASLEGEVSRSVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTN 120
           CRK    +V+ASLEGEVS++VI+KKKLAVFVSGGGSNFRSIHEASLEGL++GE VVVVTN
Sbjct: 61  CRK----VVSASLEGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGLVHGEIVVVVTN 120

Query: 121 RSGCGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLEL 180
           +S CGGAEYAR+KNIPV+LFPKTKDEPHGLSASELVATLR+FEIDFILLAG++KLIPLEL
Sbjct: 121 KSSCGGAEYARSKNIPVLLFPKTKDEPHGLSASELVATLRHFEIDFILLAGYLKLIPLEL 180

Query: 181 IQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQ 240
           IQAYSK I+NIHPSLLPAFGGKGYYGMKVHKAV++SGARFSG TIHLVDEHFDTGRILAQ
Sbjct: 181 IQAYSKCILNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQ 240

Query: 241 RVVPVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIVWRADGVPLIRSKENPNELY 296
           RVVPVL+NDSAEDLAARVLKEEHRLY EVVAALCDERI+WR+DGVPLI+SKENPNE Y
Sbjct: 241 RVVPVLSNDSAEDLAARVLKEEHRLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294

BLAST of Lsi02G028990 vs. TAIR 10
Match: AT1G31220.1 (Formyl transferase )

HSP 1 Score: 309.7 bits (792), Expect = 2.5e-84
Identity = 146/217 (67.28%), Postives = 182/217 (83.87%), Query Frame = 0

Query: 80  RKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSGCGGAEYARTKNIPVILFPK 139
           R+KKLAVFVSGGGSNFR IHE   +G + G+ V++VTN+  CGGAEYAR+  IPV++FPK
Sbjct: 76  RRKKLAVFVSGGGSNFRKIHEGCSDGSVNGDVVLLVTNKKDCGGAEYARSNGIPVLVFPK 135

Query: 140 TKDEP-HGLSASELVATLRNFEIDFILLAGFMKLIPLELIQAYSKSIVNIHPSLLPAFGG 199
            K EP  GLS SELV  LR + +DF+LLAG++KLIP+EL+QA+ K I+NIHP+LLPAFGG
Sbjct: 136 AKREPSDGLSPSELVDVLRKYGVDFVLLAGYLKLIPVELVQAFPKRILNIHPALLPAFGG 195

Query: 200 KGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKE 259
           KG YG+KVHKAV++SGAR+SGPTIH V+E +DTGRILAQ  V V+AND+ E+LA RVL E
Sbjct: 196 KGLYGIKVHKAVLESGARYSGPTIHFVNEEYDTGRILAQSAVRVIANDTPEELAKRVLHE 255

Query: 260 EHRLYVEVVAALCDERIVWRADGVPLIRSKENPNELY 296
           EH+LYVEVV A+C+ERI WR DGVPLI++K+NP+E Y
Sbjct: 256 EHKLYVEVVGAICEERIKWREDGVPLIQNKQNPDEYY 292

BLAST of Lsi02G028990 vs. TAIR 10
Match: AT4G17360.1 (Formyl transferase )

HSP 1 Score: 68.6 bits (166), Expect = 9.9e-12
Identity = 46/166 (27.71%), Postives = 78/166 (46.99%), Query Frame = 0

Query: 121 CGGAEYARTKNIPVILFPKTKDEPHGLSASELVATLRN------FEI----DFILLAGFM 180
           C  + + R  N  V+ F     + HG+S   L  T +N       E+    DF++LA +M
Sbjct: 162 CVISNHERAPNTHVMRFL----QRHGISYHYLPTTDQNKIEEEILELVKGTDFLVLARYM 221

Query: 181 KLIPLELIQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFD 240
           +L+    ++ Y K ++NIH  LLP+F G+        K   D+G +  G T H V E  D
Sbjct: 222 QLLSGNFLKGYGKDVINIHHGLLPSFKGRNPV-----KQAFDAGVKLIGATTHFVTEELD 281

Query: 241 TGRILAQRVVPVLANDSAEDLAARVLKEEHRLYVEVVAALCDERIV 277
           +G I+ Q V  V   D+      +    E +  ++ + + C+ R++
Sbjct: 282 SGPIIEQMVERVSHRDNLRSFVQKSEDLEKKCLMKAIKSYCELRVL 318

BLAST of Lsi02G028990 vs. TAIR 10
Match: AT5G47435.2 (formyltetrahydrofolate deformylase, putative )

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-11
Identity = 51/203 (25.12%), Postives = 86/203 (42.36%), Query Frame = 0

Query: 77  SVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSGCGGAEYAR---TKNIP 136
           S+  K K+A+ +S        +     +G +  +   V++N          R      IP
Sbjct: 121 SIDPKYKIALLLSKQDHCLVEMLHKWQDGKLPVDITCVISNHERASNTHVMRFLERHGIP 180

Query: 137 VILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLELIQAYSKSIVNIHPSLL 196
                 TK+        ELV      + DF++LA +M+++    ++ Y K ++NIH  LL
Sbjct: 181 YHYVSTTKENKREDDILELVK-----DTDFLVLARYMQILSGNFLKGYGKDVINIHHGLL 240

Query: 197 PAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVVPVLANDSAEDLAA 256
           P+F G GY      K   D+G +  G T H V E  D+G I+ Q V  V   D+      
Sbjct: 241 PSFKG-GYPA----KQAFDAGVKLIGATSHFVTEELDSGPIIEQMVESVSHRDNLRSFVQ 300

Query: 257 RVLKEEHRLYVEVVAALCDERIV 277
           +    E +     + + C+ R++
Sbjct: 301 KSEDLEKKCLTRAIKSYCELRVL 313

BLAST of Lsi02G028990 vs. TAIR 10
Match: AT5G47435.1 (formyltetrahydrofolate deformylase, putative )

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-11
Identity = 51/203 (25.12%), Postives = 86/203 (42.36%), Query Frame = 0

Query: 77  SVIRKKKLAVFVSGGGSNFRSIHEASLEGLIYGETVVVVTNRSGCGGAEYAR---TKNIP 136
           S+  K K+A+ +S        +     +G +  +   V++N          R      IP
Sbjct: 121 SIDPKYKIALLLSKQDHCLVEMLHKWQDGKLPVDITCVISNHERASNTHVMRFLERHGIP 180

Query: 137 VILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLELIQAYSKSIVNIHPSLL 196
                 TK+        ELV      + DF++LA +M+++    ++ Y K ++NIH  LL
Sbjct: 181 YHYVSTTKENKREDDILELVK-----DTDFLVLARYMQILSGNFLKGYGKDVINIHHGLL 240

Query: 197 PAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVVPVLANDSAEDLAA 256
           P+F G GY      K   D+G +  G T H V E  D+G I+ Q V  V   D+      
Sbjct: 241 PSFKG-GYPA----KQAFDAGVKLIGATSHFVTEELDSGPIIEQMVESVSHRDNLRSFVQ 300

Query: 257 RVLKEEHRLYVEVVAALCDERIV 277
           +    E +     + + C+ R++
Sbjct: 301 KSEDLEKKCLTRAIKSYCELRVL 313

BLAST of Lsi02G028990 vs. TAIR 10
Match: AT1G66520.1 (formyltransferase, putative )

HSP 1 Score: 46.2 bits (108), Expect = 5.2e-05
Identity = 38/151 (25.17%), Postives = 68/151 (45.03%), Query Frame = 0

Query: 124 AEYARTKNIP--VILFPKTKDEPHGLSASELVATLRNFEIDFILLAGFMKLIPLELIQAY 183
           A+YA  K +P  +I  P+   +   LSA      LR  + +  + A +  ++P + ++  
Sbjct: 79  AQYALDKGLPSDLIFSPEKAGDEAFLSA------LRELQPELCITAAYGNILPTKFLKIP 138

Query: 184 SKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGPTIHLVDEHFDTGRILAQRVVP 243
               VNIHPSLLP + G       V +A+ D G   +G ++       D G ++A +   
Sbjct: 139 VHGTVNIHPSLLPLYRG----AAPVQRALQD-GVPETGVSLAFTVRKLDAGPVIASKRFQ 198

Query: 244 VLANDSAEDLAARVLKEEHRLYVEVVAALCD 273
           V     A +L + +  E   L +  + ++ D
Sbjct: 199 VDDLIKAPELLSFLFSEGSNLLIRELPSIFD 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P524231.6e-9961.94Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Vigna unguiculata ... [more]
P524223.6e-8367.28Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Arabidopsis thalia... [more]
Q428052.6e-7361.22Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Glycine max OX=384... [more]
P009671.3e-2733.80Trifunctional purine biosynthetic protein adenosine-3 OS=Drosophila melanogaster... [more]
P218721.8e-2632.37Trifunctional purine biosynthetic protein adenosine-3 OS=Gallus gallus OX=9031 G... [more]
Match NameE-valueIdentityDescription
A0A6J1JYY48.4e-13683.05phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucurbita maxima O... [more]
A0A0A0K7R91.1e-13584.07Formyl_trans_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G450... [more]
A0A1S3CGP81.4e-13583.56phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucumis melo OX=36... [more]
A0A6J1GQR11.9e-13583.05phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucurbita moschata... [more]
A0A6J1DNE22.4e-12278.84phosphoribosylglycinamide formyltransferase, chloroplastic OS=Momordica charanti... [more]
Match NameE-valueIdentityDescription
XP_038896301.11.5e-14287.80phosphoribosylglycinamide formyltransferase, chloroplastic [Benincasa hispida][more]
XP_023547957.17.8e-13683.39phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita pepo subsp... [more]
XP_022992348.11.7e-13583.05phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita maxima][more]
XP_004141701.12.3e-13584.07phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis sativus][more]
XP_008462305.13.0e-13583.56PREDICTED: phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis m... [more]
Match NameE-valueIdentityDescription
AT1G31220.12.5e-8467.28Formyl transferase [more]
AT4G17360.19.9e-1227.71Formyl transferase [more]
AT5G47435.21.7e-1125.12formyltetrahydrofolate deformylase, putative [more]
AT5G47435.11.7e-1125.12formyltetrahydrofolate deformylase, putative [more]
AT1G66520.15.2e-0525.17formyltransferase, putative [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002376Formyl transferase, N-terminalPFAMPF00551Formyl_trans_Ncoord: 82..267
e-value: 1.4E-53
score: 181.3
NoneNo IPR availableGENE3D3.40.50.170coord: 66..293
e-value: 8.1E-65
score: 220.1
NoneNo IPR availablePANTHERPTHR43369PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASEcoord: 68..287
IPR004607Phosphoribosylglycinamide formyltransferaseTIGRFAMTIGR00639TIGR00639coord: 82..274
e-value: 2.5E-47
score: 158.9
IPR004607Phosphoribosylglycinamide formyltransferaseHAMAPMF_01930PurNcoord: 83..273
score: 26.496096
IPR004607Phosphoribosylglycinamide formyltransferaseCDDcd08645FMT_core_GARTcoord: 83..270
e-value: 1.42608E-83
score: 247.3
IPR036477Formyl transferase, N-terminal domain superfamilySUPERFAMILY53328Formyltransferasecoord: 82..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G028990.1Lsi02G028990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006189 'de novo' IMP biosynthetic process
biological_process GO:0009058 biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004644 phosphoribosylglycinamide formyltransferase activity
molecular_function GO:0016742 hydroxymethyl-, formyl- and related transferase activity