Lsi02G028920 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G028920 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 987.6 bits (2552), Expect = 7.7e-287
Identity = 500/771 (64.85%), Postives = 599/771 (77.69%), Query Frame = 0

Query: 8   SSLFFFF--ILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPH 67
           SS+FF F  +LCF     S S D  E++IVHV +S KPSLFSSHN+WH S+L SL  SP 
Sbjct: 7   SSIFFVFPLLLCFFSP-SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQ 66

Query: 68  PTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG 127
           P  LLYSY RAV+GFSA ++  Q A LR  P VISVIPD+ R++HTT TP FLG + N G
Sbjct: 67  PATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG 126

Query: 128 LWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIG 187
           LW+++NY EDVI+GVLDTGIWPE PSFSD GL P+P++WKG CE G  F AS+CNRK+IG
Sbjct: 127 LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIG 186

Query: 188 ARAYFYGY--ESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARG 247
           ARA++ GY  + N  +   + + +S RDT+GHGTHTASTAAGS V NAS +QYARG A G
Sbjct: 187 ARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATG 246

Query: 248 MASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGA 307
           MAS+ARIAAYKICW  GC+DSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DSIAIGA
Sbjct: 247 MASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGA 306

Query: 308 FGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSL 367
           FGA +HG+VVSCSAGNSGP   TA NIAPWILTVGAST+DREF A+ I GDG+VF+G SL
Sbjct: 307 FGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSL 366

Query: 368 YSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGG 427
           Y+GE L DS+L LVY GDCGSR CY G L+SS V GKIV+CDRGGNARV KGSAVK AGG
Sbjct: 367 YAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGG 426

Query: 428 LGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSP 487
            GM+LANT E+GEEL ADSHL+P TMVG  AG+++R+Y  +  +PTA I F GT+IG SP
Sbjct: 427 AGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSP 486

Query: 488 SAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGT 547
            +PRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L IDPRRV+FNIISGT
Sbjct: 487 PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGT 546

Query: 548 SMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGA 607
           SMSCPHVSG+AALLRKA P WSPAAIKSAL+TT+Y +++SG PI+DL+T K SN F+HGA
Sbjct: 547 SMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGA 606

Query: 608 GHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-HKLSNPGNL 667
           GH+DPNKALNPGL+YD+  ++YV+FLC++GY+   I VF++D +    CE  KL   G+L
Sbjct: 607 GHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDL 666

Query: 668 NYPSFAVVF-DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTT 727
           NYPSF+VVF    EVVKY R V NVG   DAVYEV V+SP  VEI V P+KL F+++K+ 
Sbjct: 667 NYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSV 726

Query: 728 QSYEITFTKI-----SGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIAS 768
             YE+TF  +      G      FGSI+W+DG H V+SP+AV +  GS+ S
Sbjct: 727 LEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of Lsi02G028920 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 3.6e-228
Identity = 426/764 (55.76%), Postives = 532/764 (69.63%), Query Frame = 0

Query: 8   SSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPT 67
           SS  FF +LC      S S  +Q T+IVH++KS+ PS F  H++W+ S L S+S S    
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---A 66

Query: 68  KLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG-L 127
           +LLY+YE A++GFS  +T  +A  L   PGVISV+P+   +LHTTRTP FLGL ++   L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 128 WADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGA 187
           + +     DV++GVLDTG+WPE  S+SDEG  P+P+SWKG CE G  F+AS CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 188 RAYFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMAS 247
           R +  GYES +G    S + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGMA 
Sbjct: 187 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 246

Query: 248 RARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGA 307
           RAR+A YK+CW  GCF SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF A
Sbjct: 247 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFAA 306

Query: 308 MQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSG 367
           M+ G++VSCSAGN+GP S +  N+APWI TVGA T+DR+F A  ILG+G+ F+GVSL+ G
Sbjct: 307 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 366

Query: 368 EPLGDSKLPLVYGGDCGS----RYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAG 427
           E L D  LP +Y G+  +      C +G+L   KV GKIV+CDRG NARV KG  VK+AG
Sbjct: 367 EALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAG 426

Query: 428 GLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNS 487
           G+GM+LANT  NGEEL+AD+HL+P T VGE AG+ +R+Y  +DPNPTA+I   GTV+G  
Sbjct: 427 GVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK 486

Query: 488 PSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISG 547
           PS P VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G + PT L+ D RRVEFNIISG
Sbjct: 487 PS-PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISG 546

Query: 548 TSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHG 607
           TSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D++T K S PF HG
Sbjct: 547 TSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHG 606

Query: 608 AGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-HKLSNPGN 667
           AGH+ P  A NPGLIYDLT +DY+ FLC++ Y S QI      S  +  C+  K  +  +
Sbjct: 607 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR---SVSRRNYTCDPSKSYSVAD 666

Query: 668 LNYPSFAVVFDDEEVVKYTRTVTNVGDETDAVYEVKVES-PQGVEISVVPNKLEFNEKKT 727
           LNYPSFAV  D     KYTRTVT+VG      Y VKV S   GV+ISV P  L F E   
Sbjct: 667 LNYPSFAVNVDGVGAYKYTRTVTSVGGA--GTYSVKVTSETTGVKISVEPAVLNFKEANE 726

Query: 728 TQSYEITFT----KISGFKELASFGSIQWSDGSHNVRSPIAVSF 761
            +SY +TFT    K SG     SFGSI+WSDG H V SP+A+S+
Sbjct: 727 KKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAISW 756

BLAST of Lsi02G028920 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 6.0e-215
Identity = 395/738 (53.52%), Postives = 505/738 (68.43%), Query Frame = 0

Query: 30  QETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPTKLLYSYERAVNGFSASITAAQA 89
           ++T+I+ V+ S+KP  F +H+ W++S L S S       LLY+Y  + +GFSA + + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  -AELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYAEDVIIGVLDTGIWPE 149
            + L     ++ +  D +  LHTTRTP FLGL    G+    + +  VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGARAYFYGYESNLGRSLRSS-DFK 209
             SF D  +  +P+ WKG CE+G  F +  CN+K+IGAR++  G++   G    S  +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEYGCFDSDIL 269
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA+RAR+A YK+CW  GCF SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGSYTA 329
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLG-GGSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEPLGDSKLPLVY--GGDCGSR 389
            N+APW++TVGA T+DR+F A   LG+G+  +GVSLYSG  +G   L LVY  G    S 
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGGLGMVLANTEENGEELLADSHLI 449
            C  GSLDSS V GKIVVCDRG NARV KG+ V+ AGGLGM++ANT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILK 509
           P   VG+  G+ LR Y  SD  PTA +VF+GTV+   PS P VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS-PVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWS 569
           PDVI PGVNILAGWS    PT L  D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDY 629
           P+AIKSAL+TT+Y LD++ +P+ D + +  SNP+ HG+GH+DP KAL+PGL+YD++ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNPGNLNYPSFAVVFDDEEVVKYTRTVTN 689
           + FLCS+ Y    I   VK  S +  C  K S+PG LNYPSF+V+F  + VV+YTR VTN
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTN 686

Query: 690 VGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTTQSYEITFTKISG--FKELASFGSI 749
           VG    +VY+V V     V ISV P+KL F      + Y +TF    G      A FGSI
Sbjct: 687 VG-AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSI 746

Query: 750 QWSDGSHNVRSPIAVSFN 762
            WS+  H VRSP+A S+N
Sbjct: 747 TWSNPQHEVRSPVAFSWN 752

BLAST of Lsi02G028920 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 1.1e-211
Identity = 391/774 (50.52%), Postives = 519/774 (67.05%), Query Frame = 0

Query: 11  FFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPTKLL 70
           +FFF+L       S S  N  T+IVHV    KPS+F +H HW++S L SL+ S  P  ++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPSII 65

Query: 71  YSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL--ADNLGLWA 130
           ++Y+   +GFSA +T+  A++L   P VISVIP+++R LHTTR+P FLGL   D  GL  
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 DTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGARA 190
           ++++  D++IGV+DTG+WPERPSF D GL PVP  WKG C   + F  SACNRK++GAR 
Sbjct: 126 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 185

Query: 191 YFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRA 250
           +  GYE+  G+   +++F+S RD+DGHGTHTAS +AG +V  AS   YA G A GMA +A
Sbjct: 186 FCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKA 245

Query: 251 RIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQ 310
           R+AAYK+CW  GC+DSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFGA+ 
Sbjct: 246 RLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGAID 305

Query: 311 HGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEP 370
            G+ VS SAGN GPG+ T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G  
Sbjct: 306 RGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPG 365

Query: 371 LGDSKL-PLVYGGDC------GSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSA 430
           L   ++ PLVYGG         S  C  GSLD + V GKIV+CDRG N+R  KG  V+  
Sbjct: 366 LDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKN 425

Query: 431 GGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNY------THSDPNPTATIVFR 490
           GGLGM++AN   +GE L+AD H++P T VG   G+++R Y      + S  +PTATIVF+
Sbjct: 426 GGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFK 485

Query: 491 GTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRV 550
           GT +G  P AP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+ ++ D RR 
Sbjct: 486 GTRLGIRP-APVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 545

Query: 551 EFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKE 610
           EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y++D+SG P+ D ST   
Sbjct: 546 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 605

Query: 611 SNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-- 670
           S+   +G+GH+ P KA++PGL+YD+T  DY++FLC+  Y    I    +  +    C+  
Sbjct: 606 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD---CDGA 665

Query: 671 HKLSNPGNLNYPSFAVVF----DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVV 730
            +  + GNLNYPSF+VVF    + +    + RTVTNVGD +D+VYE+K+  P+G  ++V 
Sbjct: 666 RRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVTVE 725

Query: 731 PNKLEFNEKKTTQSYEI----TFTKISGFKELASFGSIQWSDGSHNVRSPIAVS 760
           P KL F       S+ +    T  K+S        G I WSDG  NV SP+ V+
Sbjct: 726 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Lsi02G028920 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 7.4e-205
Identity = 380/773 (49.16%), Postives = 512/773 (66.24%), Query Frame = 0

Query: 12  FFFILCFVPVIFSRSP-----DNQETFIVHVSKSEKPSLFSSHNHWHSSILESLS----- 71
           F FI+  + +IF ++        ++T+++H+ KS  P  +++H  W+SS + S++     
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 72  -PSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL 131
               +  ++LY+Y+ A +G +A +T  +A  L    GV++VIP+   +LHTTR+P FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 132 --ADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASA 191
              ++  +WA+     DV++GVLDTGIWPE  SF+D G+SPVPA+W+G CETG+ F    
Sbjct: 131 ERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRN 190

Query: 192 CNRKIIGARAYFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 251
           CNRKI+GAR ++ GYE+  G+     ++KS RD DGHGTHTA+T AGS VK A+ F +A 
Sbjct: 191 CNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAY 250

Query: 252 GEARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 311
           G ARGMA +AR+AAYK+CW  GCF SDIL+A+DQA+ADGV ++S+S+G  G    Y RDS
Sbjct: 251 GTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLG--GGVSTYSRDS 310

Query: 312 IAIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 371
           ++I  FGAM+ GV VSCSAGN GP   +  N++PWI TVGAST+DR+F A V +G  R F
Sbjct: 311 LSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTF 370

Query: 372 SGVSLYSGEPL--GDSKLPLVYGGDCGS-----RYCYSGSLDSSKVAGKIVVCDRGGNAR 431
            GVSLY G  +   + + PLVY G   S      +C  G+LD   VAGKIV+CDRG   R
Sbjct: 371 KGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPR 430

Query: 432 VAKGSAVKSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTAT 491
           V KG  VK AGG+GMVL NT  NGEEL+ADSH++P   VGE  G  ++ Y  +    TA+
Sbjct: 431 VQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATAS 490

Query: 492 IVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSID 551
           +   GT IG  PS P VAAFSSRGPN+ + EILKPD++APGVNILA W+G  +P+SLS D
Sbjct: 491 LEILGTRIGIKPS-PVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSD 550

Query: 552 PRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLS 611
           PRRV+FNI+SGTSMSCPHVSGVAAL++   P WSPAAIKSAL+TT+Y  D+   P+ D S
Sbjct: 551 PRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDAS 610

Query: 612 TSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQL 671
            +  S+P+ HGAGHIDP +A +PGL+YD+ PQ+Y  FLC+      Q+ VF K S  ++ 
Sbjct: 611 GAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHS--NRT 670

Query: 672 CEHKLS-NPGNLNYPSFAVVFDDEEVVK---YTRTVTNVGDETDAVYEVKVESPQGVEIS 731
           C+H L+ NPGNLNYP+ + +F +   VK     RTVTNVG    + Y+V V   +G  ++
Sbjct: 671 CKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISS-YKVSVSPFKGASVT 730

Query: 732 VVPNKLEFNEKKTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSF 761
           V P  L F  K    SY +TF      K    FG + W   +H VRSP+ +++
Sbjct: 731 VQPKTLNFTSKHQKLSYTVTFRTRFRMKR-PEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Lsi02G028920 vs. ExPASy TrEMBL
Match: A0A6J1JUV1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111488562 PE=3 SV=1)

HSP 1 Score: 1394.4 bits (3608), Expect = 0.0e+00
Identity = 698/771 (90.53%), Postives = 732/771 (94.94%), Query Frame = 0

Query: 1   MANFHLCSSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESL 60
           MANFH CSS+FFFF LCF+PVIFSRSPD QETFIVHVSKSEKPSLFSSH  WHSSIL+SL
Sbjct: 1   MANFHRCSSVFFFFFLCFLPVIFSRSPDKQETFIVHVSKSEKPSLFSSHYRWHSSILDSL 60

Query: 61  SPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL 120
           SPS  P KLLY+YERAVNGF+A ITA QAAELR VPGV+ VIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSSRPRKLLYNYERAVNGFAARITADQAAELRRVPGVVLVIPDRMRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACN 180
           ADNLGLW DTNYA+DVIIGVLDTGIWPE PSFSDEGLSPVPASWKGTCE GEGFSA+ CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPEHPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180

Query: 181 RKIIGARAYFYGYESNLGRSLRS-SDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARA+F+GYESN+GR L   SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAFFHGYESNIGRRLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWE+GC+DSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHG+VVSCSAGNSGPG YTAVNIAPWILTVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSG+PLGDSKLPLVYGGDCG+RYCYSGSLD SKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVI 480
           SAGGLG++LANTEENGEELLADSHLIPGTM+GEIAGNKLR+Y HSDPNP+ATIVFRGTVI
Sbjct: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480

Query: 481 GNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           G+SP APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSALITTSYSLD+SGSPIKDLSTS +SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGSPIKDLSTSNDSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNP 660
           VHG GHIDPNKALNPGLIYDL P DYVSFLCSIGYDSRQIAVFVKDSSYS LCEHKLSNP
Sbjct: 601 VHGGGHIDPNKALNPGLIYDLNPHDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660

Query: 661 GNLNYPSFAVVF--DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNE 720
           GNLNYPSFAVVF  + EEVVKYTRTVTNVGDE DAVYEVKVE+PQGVEISV+PNKLEF+ 
Sbjct: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
           KKTT+SYEITFTKISGFK  ASFGSIQWSDGSH VRSPIAVSFNT SIASM
Sbjct: 721 KKTTESYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771

BLAST of Lsi02G028920 vs. ExPASy TrEMBL
Match: A0A1S3CGN8 (LOW QUALITY PROTEIN: subtilisin-like protease SBT1.4 OS=Cucumis melo OX=3656 GN=LOC103500685 PE=3 SV=1)

HSP 1 Score: 1393.3 bits (3605), Expect = 0.0e+00
Identity = 699/770 (90.78%), Postives = 736/770 (95.58%), Query Frame = 0

Query: 1   MANFHLCSSLFFFFILCFV-PVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILES 60
           MANFHL SS FFFF + FV P++FSRS + QET+IVHVSKSEKPSLFSSH+HWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLY+YERA NGFSA IT AQA ELR VPG+ISVIPDRIRQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASAC 180
           LADNLGLWADTNYA+DVIIGVLDTGIWPERPSFSDEGL+PVPA WKGTC+TGEG SA AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLR-SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F GYESNL  SL+ SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFXGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWE+GC+DSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPG YTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360

Query: 361 SGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSG+PLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAG+IVVCDRGGNARVAKG AV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLR+Y H+DPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IG+SP AP+VA+FSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+IDPRRVEFN
Sbjct: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDL+TS+ESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSN 660
           FVHGAGHIDPN+ALNPGLIYDLTPQDYVSFLCSIGYDS+QIAVFVK SSYSQLCEHKLSN
Sbjct: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEK 720
           PGNLNYPSF+VVFDD EVVKYTRTVTNVGDET+ VY VKVE+PQGVEISVVPNKLEFN++
Sbjct: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
           KTT SYEITFTKI+GFKE ASFGSIQWSDG HNVRSPIAVSF TGSIASM
Sbjct: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 770

BLAST of Lsi02G028920 vs. ExPASy TrEMBL
Match: A0A6J1HMN7 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111464338 PE=3 SV=1)

HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 698/771 (90.53%), Postives = 732/771 (94.94%), Query Frame = 0

Query: 1   MANFHLCSSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESL 60
           MAN HLCSS FFF ILCFVPVIFSRS  NQETFIVHVSKSEKP+LFSSH+HWHSSILESL
Sbjct: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL 120
           SPSPHPTKLLYSYE A NGFSA ITAAQAAELR +PGVISVIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACN 180
           AD+ GLWA+TNYA+DVIIGVLDTGIWPERPSFSDEGLSPVPA+WKGTC+TG GFSASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFYGYESNLGRSLR-SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYF GYES + RSL+ SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWE GC+DSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPG YTAVNIAPWI+TVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSG+PLGDSKLPLVY GDCG RYCYSG+LDSSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVI 480
           +AGGLGM+LANTEENGEELLADSHLIPGTM+GEIAGN L++Y HSDP+PTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           G+SP APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSAL+TTSYSLDSSG PI DLSTSK+SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNP 660
           VHGAGHIDPNKALNPGLIYDL PQDYVSFLCSIGYDSRQIAVFVKDS Y+QLCEHKL++P
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFDD--EEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNE 720
           GNLNYPSFAVVFD   +EVVKYTRTVTNVGDE DAVYEVKVESPQGVEI+V+PNKLEF+ 
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
           K  TQSYEITFTKI+G K  ASFGSIQWSDGSH VRSP+AVSFN+G IASM
Sbjct: 721 KDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Lsi02G028920 vs. ExPASy TrEMBL
Match: A0A6J1GQK5 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111456188 PE=3 SV=1)

HSP 1 Score: 1389.0 bits (3594), Expect = 0.0e+00
Identity = 697/772 (90.28%), Postives = 732/772 (94.82%), Query Frame = 0

Query: 1   MANFHLCSSL-FFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILES 60
           MANFH CSS+ FFFF LCF+PVIFSRSPD QETFIVHVSK EKPSLFSSH  WHSSIL+S
Sbjct: 1   MANFHRCSSVFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILDS 60

Query: 61  LSPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPS  P KLLY+YERAVNGF+A IT AQAAELR VPGV+SVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASAC 180
           LADNLGLW DTNYA+DVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCE GEGFSA+ C
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLRS-SDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F+GYESN+GR L   SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWE+GC+DSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPG YTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSG+PLGDSKLPLVYGGDCG+RYCYSGSLD SKVAGKIVVCDRGGNARVAKGSAV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTV 480
           KSAGGLG++LANTEENGEELLADSHLIPGTM+GEIAGNKLR+Y HSDPNP+ATIVFRGT+
Sbjct: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTI 480

Query: 481 IGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           I +SP +PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEFN
Sbjct: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSALITTSYSLD+SG PIKDLSTS +SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSN 660
           FVHG GHIDPNKALNPGLIYDL PQDYVSFLCSIGYDSRQIAVFVKDSSYS LCEHKLSN
Sbjct: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660

Query: 661 PGNLNYPSFAVVF--DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFN 720
           PGNLNYPSFAVVF  + EEVVKYTRTVTNVGDE DAVYEVKVE+PQGVEISV+PNKLEF+
Sbjct: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFS 720

Query: 721 EKKTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
            KKTTQSYEITFTKISGFK  ASFGSIQWSDGSH VRSPIAVSFNT SIASM
Sbjct: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 772

BLAST of Lsi02G028920 vs. ExPASy TrEMBL
Match: A0A0A0K6Z1 (Subtilase family protein OS=Cucumis sativus OX=3659 GN=Csa_7G448910 PE=3 SV=1)

HSP 1 Score: 1388.2 bits (3592), Expect = 0.0e+00
Identity = 699/771 (90.66%), Postives = 733/771 (95.07%), Query Frame = 0

Query: 1   MANFHL-CSSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILES 60
           MANFHL  SS FFFF+ CF P+IFSRS +NQET+IVHVSKSEKPSLFSSH+HWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           LS SPHPTKLLY+YERA NGFSA IT  QA ELR VPG+ISVIPD+IRQLHTTRTPHFLG
Sbjct: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASAC 180
           LADNLGLWADTNYA+DVIIGVLDTGIWPERPSFSDEGLSPVPA WKGTC+TGEG SA AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLR-SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAYFYGYESNL  SL+ SSDFKSARDT+GHGTHTASTAAGSFV NASFFQYAR
Sbjct: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWE+GC+DSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPG YTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSG+PLGDSKL LVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKG AV
Sbjct: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVG IAGNKLR+Y H+DPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IG+SP APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+IDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSG PIKDLSTS+ESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSN 660
           FVHGAGHI+PN+ALNPGLIYDLTPQDYVSFLCSIGYDS+QIAVFVK SSY QLCEHKL+N
Sbjct: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660

Query: 661 PGNLNYPSFAVVFDDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEK 720
           PGNLNYPSF+VVFD+EEVVKYTRTVTNVGDET+ VYEVKVE+PQGV ISVVPNKLEFN++
Sbjct: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNT-GSIASM 769
           KTTQSYEITFTKI+GFKE ASFGSIQW DG H+VRSPIAVSF T GSIASM
Sbjct: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771

BLAST of Lsi02G028920 vs. NCBI nr
Match: XP_038897980.1 (subtilisin-like protease SBT1.4 [Benincasa hispida])

HSP 1 Score: 1435.6 bits (3715), Expect = 0.0e+00
Identity = 718/769 (93.37%), Postives = 742/769 (96.49%), Query Frame = 0

Query: 1   MANFHLCSSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESL 60
           MA  HLCSSLFF FILCF PVIFSRSP+NQETFIVHVSKSEKPSLFSSH+HWHSSILESL
Sbjct: 1   MAKSHLCSSLFFLFILCFAPVIFSRSPENQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60

Query: 61  SPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL 120
           SPSPHPTKLLY+YERAVNGFSA +TAAQAA+LRHVPGVISVIPDRIRQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYNYERAVNGFSARLTAAQAAQLRHVPGVISVIPDRIRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACN 180
           ADNLGLW DTNYA+DVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSAS CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASFCN 180

Query: 181 RKIIGARAYFYGYESNLGRSLR-SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYFYGY SN GRS   SSDFKSARDT+GHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFYGYASNFGRSFNGSSDFKSARDTEGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWE+GCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHG+VVSCSAGNSGPG YTAVNIAPWILTVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSG+PLGDSK+PLVYGGDCGSRYCYSGSL+ SKVAGKIVVCDRGGNARVAKG AVK
Sbjct: 361 GVSLYSGDPLGDSKVPLVYGGDCGSRYCYSGSLNPSKVAGKIVVCDRGGNARVAKGGAVK 420

Query: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVI 480
           SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLR+Y HSDPNPTA IVFRGTVI
Sbjct: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHSDPNPTAIIVFRGTVI 480

Query: 481 GNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           G+SP APRVAAFSSRGPNYRTAEILKPDVIAPGVNILA WSGYSSPT L+IDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILASWSGYSSPTGLNIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTS+ESNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSEESNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNP 660
           VHGAGHIDPNKALNPGLIYD++PQDYVSFLCSIGYDS+QIAVFVKDSSYSQLCEHKLSNP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDISPQDYVSFLCSIGYDSKQIAVFVKDSSYSQLCEHKLSNP 660

Query: 661 GNLNYPSFAVVFDDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEKK 720
           GNLNYPSFAVVF+ E+VVKYTRTVTNVGDE+D VYEVKVE+PQGVEISVVPNKL FN +K
Sbjct: 661 GNLNYPSFAVVFNGEDVVKYTRTVTNVGDESDVVYEVKVEAPQGVEISVVPNKLSFNAEK 720

Query: 721 TTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
           TT SYEITFTKISGF E ASFGSIQWSDGSHNVRSPIAVSFNTGSIASM
Sbjct: 721 TTDSYEITFTKISGFVEAASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769

BLAST of Lsi02G028920 vs. NCBI nr
Match: XP_022992130.1 (subtilisin-like protease SBT1.4 [Cucurbita maxima])

HSP 1 Score: 1394.4 bits (3608), Expect = 0.0e+00
Identity = 698/771 (90.53%), Postives = 732/771 (94.94%), Query Frame = 0

Query: 1   MANFHLCSSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESL 60
           MANFH CSS+FFFF LCF+PVIFSRSPD QETFIVHVSKSEKPSLFSSH  WHSSIL+SL
Sbjct: 1   MANFHRCSSVFFFFFLCFLPVIFSRSPDKQETFIVHVSKSEKPSLFSSHYRWHSSILDSL 60

Query: 61  SPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL 120
           SPS  P KLLY+YERAVNGF+A ITA QAAELR VPGV+ VIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSSRPRKLLYNYERAVNGFAARITADQAAELRRVPGVVLVIPDRMRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACN 180
           ADNLGLW DTNYA+DVIIGVLDTGIWPE PSFSDEGLSPVPASWKGTCE GEGFSA+ CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPEHPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180

Query: 181 RKIIGARAYFYGYESNLGRSLRS-SDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARA+F+GYESN+GR L   SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAFFHGYESNIGRRLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWE+GC+DSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHG+VVSCSAGNSGPG YTAVNIAPWILTVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSG+PLGDSKLPLVYGGDCG+RYCYSGSLD SKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVI 480
           SAGGLG++LANTEENGEELLADSHLIPGTM+GEIAGNKLR+Y HSDPNP+ATIVFRGTVI
Sbjct: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480

Query: 481 GNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           G+SP APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSALITTSYSLD+SGSPIKDLSTS +SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGSPIKDLSTSNDSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNP 660
           VHG GHIDPNKALNPGLIYDL P DYVSFLCSIGYDSRQIAVFVKDSSYS LCEHKLSNP
Sbjct: 601 VHGGGHIDPNKALNPGLIYDLNPHDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660

Query: 661 GNLNYPSFAVVF--DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNE 720
           GNLNYPSFAVVF  + EEVVKYTRTVTNVGDE DAVYEVKVE+PQGVEISV+PNKLEF+ 
Sbjct: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
           KKTT+SYEITFTKISGFK  ASFGSIQWSDGSH VRSPIAVSFNT SIASM
Sbjct: 721 KKTTESYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771

BLAST of Lsi02G028920 vs. NCBI nr
Match: XP_008462294.1 (PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT1.4 [Cucumis melo])

HSP 1 Score: 1393.3 bits (3605), Expect = 0.0e+00
Identity = 699/770 (90.78%), Postives = 736/770 (95.58%), Query Frame = 0

Query: 1   MANFHLCSSLFFFFILCFV-PVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILES 60
           MANFHL SS FFFF + FV P++FSRS + QET+IVHVSKSEKPSLFSSH+HWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLY+YERA NGFSA IT AQA ELR VPG+ISVIPDRIRQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASAC 180
           LADNLGLWADTNYA+DVIIGVLDTGIWPERPSFSDEGL+PVPA WKGTC+TGEG SA AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLR-SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F GYESNL  SL+ SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFXGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWE+GC+DSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPG YTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360

Query: 361 SGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSG+PLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAG+IVVCDRGGNARVAKG AV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLR+Y H+DPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IG+SP AP+VA+FSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+IDPRRVEFN
Sbjct: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDL+TS+ESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSN 660
           FVHGAGHIDPN+ALNPGLIYDLTPQDYVSFLCSIGYDS+QIAVFVK SSYSQLCEHKLSN
Sbjct: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEK 720
           PGNLNYPSF+VVFDD EVVKYTRTVTNVGDET+ VY VKVE+PQGVEISVVPNKLEFN++
Sbjct: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
           KTT SYEITFTKI+GFKE ASFGSIQWSDG HNVRSPIAVSF TGSIASM
Sbjct: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 770

BLAST of Lsi02G028920 vs. NCBI nr
Match: KAG6575439.1 (Subtilisin-like protease 1.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1390.2 bits (3597), Expect = 0.0e+00
Identity = 698/773 (90.30%), Postives = 732/773 (94.70%), Query Frame = 0

Query: 1   MANFHLCSSL--FFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILE 60
           MANFH CSS+  FFFF LCF+PVIFSRSPD QETFIVHVSK EKPSLFSSH  WHSSIL+
Sbjct: 1   MANFHRCSSVFFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILD 60

Query: 61  SLSPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFL 120
           SLSPS  P KLLY+YERAVNGF+A IT AQAAELR VPGV+SVIPDR+RQLHTTRTPHFL
Sbjct: 61  SLSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFL 120

Query: 121 GLADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASA 180
           GLADNLGLW DTNYA+DVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCE GEGFSA+ 
Sbjct: 121 GLADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATT 180

Query: 181 CNRKIIGARAYFYGYESNLGRSLRS-SDFKSARDTDGHGTHTASTAAGSFVKNASFFQYA 240
           CNRKIIGARA+F+GYESN+GR L   SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYA
Sbjct: 181 CNRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYA 240

Query: 241 RGEARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRD 300
           RGEARGMASRARIAAYKICWE+GC+DSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRD
Sbjct: 241 RGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRD 300

Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
           SIAIGAFGAMQHG+VVSCSAGNSGPG YTAVNIAPWILTVGASTIDREFLADVILGDGRV
Sbjct: 301 SIAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360

Query: 361 FSGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSA 420
           FSGVSLYSG+PLGDSKLPLVYGGDCG+RYCYSGSLD SKVAGKIVVCDRGGNARVAKGSA
Sbjct: 361 FSGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSA 420

Query: 421 VKSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGT 480
           VKSAGGLG++LANTEENGEELLADSHLIPGTM+GEIAGNKLR+Y HSDPNP+ATIVFRGT
Sbjct: 421 VKSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGT 480

Query: 481 VIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEF 540
           VI +SP +PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEF
Sbjct: 481 VISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEF 540

Query: 541 NIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESN 600
           NIISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSALITTSYSLD+SG PIKDLSTS +SN
Sbjct: 541 NIISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSN 600

Query: 601 PFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLS 660
           PFVHG GHIDPNKALNPGLIYDL PQDYVSFLCSIGYDSRQIAVFVKDSSYS LCEHKLS
Sbjct: 601 PFVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLS 660

Query: 661 NPGNLNYPSFAVVF--DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEF 720
           NPGNLNYPSFAVVF  + EEVVKYTRTVTNVGDE DAVYEVKVE+PQGVEISV+PNKLEF
Sbjct: 661 NPGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEF 720

Query: 721 NEKKTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
           + KKTTQSYEITFTKISGFK  ASFGSIQWSDGSH VRSPIAVSFNT SIASM
Sbjct: 721 SAKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHTVRSPIAVSFNTASIASM 773

BLAST of Lsi02G028920 vs. NCBI nr
Match: XP_022964269.1 (subtilisin-like protease SBT1.4 [Cucurbita moschata])

HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 698/771 (90.53%), Postives = 732/771 (94.94%), Query Frame = 0

Query: 1   MANFHLCSSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESL 60
           MAN HLCSS FFF ILCFVPVIFSRS  NQETFIVHVSKSEKP+LFSSH+HWHSSILESL
Sbjct: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL 120
           SPSPHPTKLLYSYE A NGFSA ITAAQAAELR +PGVISVIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACN 180
           AD+ GLWA+TNYA+DVIIGVLDTGIWPERPSFSDEGLSPVPA+WKGTC+TG GFSASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFYGYESNLGRSLR-SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYF GYES + RSL+ SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWE GC+DSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPG YTAVNIAPWI+TVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSG+PLGDSKLPLVY GDCG RYCYSG+LDSSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVI 480
           +AGGLGM+LANTEENGEELLADSHLIPGTM+GEIAGN L++Y HSDP+PTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           G+SP APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSAL+TTSYSLDSSG PI DLSTSK+SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNP 660
           VHGAGHIDPNKALNPGLIYDL PQDYVSFLCSIGYDSRQIAVFVKDS Y+QLCEHKL++P
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFDD--EEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNE 720
           GNLNYPSFAVVFD   +EVVKYTRTVTNVGDE DAVYEVKVESPQGVEI+V+PNKLEF+ 
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
           K  TQSYEITFTKI+G K  ASFGSIQWSDGSH VRSP+AVSFN+G IASM
Sbjct: 721 KDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Lsi02G028920 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 987.6 bits (2552), Expect = 5.5e-288
Identity = 500/771 (64.85%), Postives = 599/771 (77.69%), Query Frame = 0

Query: 8   SSLFFFF--ILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPH 67
           SS+FF F  +LCF     S S D  E++IVHV +S KPSLFSSHN+WH S+L SL  SP 
Sbjct: 7   SSIFFVFPLLLCFFSP-SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQ 66

Query: 68  PTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG 127
           P  LLYSY RAV+GFSA ++  Q A LR  P VISVIPD+ R++HTT TP FLG + N G
Sbjct: 67  PATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG 126

Query: 128 LWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIG 187
           LW+++NY EDVI+GVLDTGIWPE PSFSD GL P+P++WKG CE G  F AS+CNRK+IG
Sbjct: 127 LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIG 186

Query: 188 ARAYFYGY--ESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARG 247
           ARA++ GY  + N  +   + + +S RDT+GHGTHTASTAAGS V NAS +QYARG A G
Sbjct: 187 ARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATG 246

Query: 248 MASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGA 307
           MAS+ARIAAYKICW  GC+DSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DSIAIGA
Sbjct: 247 MASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGA 306

Query: 308 FGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSL 367
           FGA +HG+VVSCSAGNSGP   TA NIAPWILTVGAST+DREF A+ I GDG+VF+G SL
Sbjct: 307 FGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSL 366

Query: 368 YSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGG 427
           Y+GE L DS+L LVY GDCGSR CY G L+SS V GKIV+CDRGGNARV KGSAVK AGG
Sbjct: 367 YAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGG 426

Query: 428 LGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSP 487
            GM+LANT E+GEEL ADSHL+P TMVG  AG+++R+Y  +  +PTA I F GT+IG SP
Sbjct: 427 AGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSP 486

Query: 488 SAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGT 547
            +PRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L IDPRRV+FNIISGT
Sbjct: 487 PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGT 546

Query: 548 SMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGA 607
           SMSCPHVSG+AALLRKA P WSPAAIKSAL+TT+Y +++SG PI+DL+T K SN F+HGA
Sbjct: 547 SMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGA 606

Query: 608 GHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-HKLSNPGNL 667
           GH+DPNKALNPGL+YD+  ++YV+FLC++GY+   I VF++D +    CE  KL   G+L
Sbjct: 607 GHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDL 666

Query: 668 NYPSFAVVF-DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTT 727
           NYPSF+VVF    EVVKY R V NVG   DAVYEV V+SP  VEI V P+KL F+++K+ 
Sbjct: 667 NYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSV 726

Query: 728 QSYEITFTKI-----SGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIAS 768
             YE+TF  +      G      FGSI+W+DG H V+SP+AV +  GS+ S
Sbjct: 727 LEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of Lsi02G028920 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 792.7 bits (2046), Expect = 2.6e-229
Identity = 426/764 (55.76%), Postives = 532/764 (69.63%), Query Frame = 0

Query: 8   SSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPT 67
           SS  FF +LC      S S  +Q T+IVH++KS+ PS F  H++W+ S L S+S S    
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---A 66

Query: 68  KLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG-L 127
           +LLY+YE A++GFS  +T  +A  L   PGVISV+P+   +LHTTRTP FLGL ++   L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 128 WADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGA 187
           + +     DV++GVLDTG+WPE  S+SDEG  P+P+SWKG CE G  F+AS CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 188 RAYFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMAS 247
           R +  GYES +G    S + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGMA 
Sbjct: 187 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 246

Query: 248 RARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGA 307
           RAR+A YK+CW  GCF SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF A
Sbjct: 247 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFAA 306

Query: 308 MQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSG 367
           M+ G++VSCSAGN+GP S +  N+APWI TVGA T+DR+F A  ILG+G+ F+GVSL+ G
Sbjct: 307 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 366

Query: 368 EPLGDSKLPLVYGGDCGS----RYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAG 427
           E L D  LP +Y G+  +      C +G+L   KV GKIV+CDRG NARV KG  VK+AG
Sbjct: 367 EALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAG 426

Query: 428 GLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNS 487
           G+GM+LANT  NGEEL+AD+HL+P T VGE AG+ +R+Y  +DPNPTA+I   GTV+G  
Sbjct: 427 GVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK 486

Query: 488 PSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISG 547
           PS P VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G + PT L+ D RRVEFNIISG
Sbjct: 487 PS-PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISG 546

Query: 548 TSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHG 607
           TSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D++T K S PF HG
Sbjct: 547 TSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHG 606

Query: 608 AGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-HKLSNPGN 667
           AGH+ P  A NPGLIYDLT +DY+ FLC++ Y S QI      S  +  C+  K  +  +
Sbjct: 607 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR---SVSRRNYTCDPSKSYSVAD 666

Query: 668 LNYPSFAVVFDDEEVVKYTRTVTNVGDETDAVYEVKVES-PQGVEISVVPNKLEFNEKKT 727
           LNYPSFAV  D     KYTRTVT+VG      Y VKV S   GV+ISV P  L F E   
Sbjct: 667 LNYPSFAVNVDGVGAYKYTRTVTSVGGA--GTYSVKVTSETTGVKISVEPAVLNFKEANE 726

Query: 728 TQSYEITFT----KISGFKELASFGSIQWSDGSHNVRSPIAVSF 761
            +SY +TFT    K SG     SFGSI+WSDG H V SP+A+S+
Sbjct: 727 KKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAISW 756

BLAST of Lsi02G028920 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 748.8 bits (1932), Expect = 4.3e-216
Identity = 395/738 (53.52%), Postives = 505/738 (68.43%), Query Frame = 0

Query: 30  QETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPTKLLYSYERAVNGFSASITAAQA 89
           ++T+I+ V+ S+KP  F +H+ W++S L S S       LLY+Y  + +GFSA + + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  -AELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYAEDVIIGVLDTGIWPE 149
            + L     ++ +  D +  LHTTRTP FLGL    G+    + +  VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGARAYFYGYESNLGRSLRSS-DFK 209
             SF D  +  +P+ WKG CE+G  F +  CN+K+IGAR++  G++   G    S  +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEYGCFDSDIL 269
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA+RAR+A YK+CW  GCF SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGSYTA 329
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLG-GGSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEPLGDSKLPLVY--GGDCGSR 389
            N+APW++TVGA T+DR+F A   LG+G+  +GVSLYSG  +G   L LVY  G    S 
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGGLGMVLANTEENGEELLADSHLI 449
            C  GSLDSS V GKIVVCDRG NARV KG+ V+ AGGLGM++ANT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILK 509
           P   VG+  G+ LR Y  SD  PTA +VF+GTV+   PS P VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS-PVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWS 569
           PDVI PGVNILAGWS    PT L  D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDY 629
           P+AIKSAL+TT+Y LD++ +P+ D + +  SNP+ HG+GH+DP KAL+PGL+YD++ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNPGNLNYPSFAVVFDDEEVVKYTRTVTN 689
           + FLCS+ Y    I   VK  S +  C  K S+PG LNYPSF+V+F  + VV+YTR VTN
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTN 686

Query: 690 VGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTTQSYEITFTKISG--FKELASFGSI 749
           VG    +VY+V V     V ISV P+KL F      + Y +TF    G      A FGSI
Sbjct: 687 VG-AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSI 746

Query: 750 QWSDGSHNVRSPIAVSFN 762
            WS+  H VRSP+A S+N
Sbjct: 747 TWSNPQHEVRSPVAFSWN 752

BLAST of Lsi02G028920 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 738.0 bits (1904), Expect = 7.5e-213
Identity = 391/774 (50.52%), Postives = 519/774 (67.05%), Query Frame = 0

Query: 11  FFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPTKLL 70
           +FFF+L       S S  N  T+IVHV    KPS+F +H HW++S L SL+ S  P  ++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPSII 65

Query: 71  YSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL--ADNLGLWA 130
           ++Y+   +GFSA +T+  A++L   P VISVIP+++R LHTTR+P FLGL   D  GL  
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 DTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGARA 190
           ++++  D++IGV+DTG+WPERPSF D GL PVP  WKG C   + F  SACNRK++GAR 
Sbjct: 126 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 185

Query: 191 YFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRA 250
           +  GYE+  G+   +++F+S RD+DGHGTHTAS +AG +V  AS   YA G A GMA +A
Sbjct: 186 FCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKA 245

Query: 251 RIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQ 310
           R+AAYK+CW  GC+DSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFGA+ 
Sbjct: 246 RLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGAID 305

Query: 311 HGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEP 370
            G+ VS SAGN GPG+ T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G  
Sbjct: 306 RGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPG 365

Query: 371 LGDSKL-PLVYGGDC------GSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSA 430
           L   ++ PLVYGG         S  C  GSLD + V GKIV+CDRG N+R  KG  V+  
Sbjct: 366 LDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKN 425

Query: 431 GGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNY------THSDPNPTATIVFR 490
           GGLGM++AN   +GE L+AD H++P T VG   G+++R Y      + S  +PTATIVF+
Sbjct: 426 GGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFK 485

Query: 491 GTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRV 550
           GT +G  P AP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+ ++ D RR 
Sbjct: 486 GTRLGIRP-APVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 545

Query: 551 EFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKE 610
           EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y++D+SG P+ D ST   
Sbjct: 546 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 605

Query: 611 SNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-- 670
           S+   +G+GH+ P KA++PGL+YD+T  DY++FLC+  Y    I    +  +    C+  
Sbjct: 606 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD---CDGA 665

Query: 671 HKLSNPGNLNYPSFAVVF----DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVV 730
            +  + GNLNYPSF+VVF    + +    + RTVTNVGD +D+VYE+K+  P+G  ++V 
Sbjct: 666 RRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVTVE 725

Query: 731 PNKLEFNEKKTTQSYEI----TFTKISGFKELASFGSIQWSDGSHNVRSPIAVS 760
           P KL F       S+ +    T  K+S        G I WSDG  NV SP+ V+
Sbjct: 726 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Lsi02G028920 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 715.3 bits (1845), Expect = 5.2e-206
Identity = 380/773 (49.16%), Postives = 512/773 (66.24%), Query Frame = 0

Query: 12  FFFILCFVPVIFSRSP-----DNQETFIVHVSKSEKPSLFSSHNHWHSSILESLS----- 71
           F FI+  + +IF ++        ++T+++H+ KS  P  +++H  W+SS + S++     
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 72  -PSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL 131
               +  ++LY+Y+ A +G +A +T  +A  L    GV++VIP+   +LHTTR+P FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 132 --ADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASA 191
              ++  +WA+     DV++GVLDTGIWPE  SF+D G+SPVPA+W+G CETG+ F    
Sbjct: 131 ERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRN 190

Query: 192 CNRKIIGARAYFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 251
           CNRKI+GAR ++ GYE+  G+     ++KS RD DGHGTHTA+T AGS VK A+ F +A 
Sbjct: 191 CNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAY 250

Query: 252 GEARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 311
           G ARGMA +AR+AAYK+CW  GCF SDIL+A+DQA+ADGV ++S+S+G  G    Y RDS
Sbjct: 251 GTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLG--GGVSTYSRDS 310

Query: 312 IAIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 371
           ++I  FGAM+ GV VSCSAGN GP   +  N++PWI TVGAST+DR+F A V +G  R F
Sbjct: 311 LSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTF 370

Query: 372 SGVSLYSGEPL--GDSKLPLVYGGDCGS-----RYCYSGSLDSSKVAGKIVVCDRGGNAR 431
            GVSLY G  +   + + PLVY G   S      +C  G+LD   VAGKIV+CDRG   R
Sbjct: 371 KGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPR 430

Query: 432 VAKGSAVKSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTAT 491
           V KG  VK AGG+GMVL NT  NGEEL+ADSH++P   VGE  G  ++ Y  +    TA+
Sbjct: 431 VQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATAS 490

Query: 492 IVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSID 551
           +   GT IG  PS P VAAFSSRGPN+ + EILKPD++APGVNILA W+G  +P+SLS D
Sbjct: 491 LEILGTRIGIKPS-PVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSD 550

Query: 552 PRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLS 611
           PRRV+FNI+SGTSMSCPHVSGVAAL++   P WSPAAIKSAL+TT+Y  D+   P+ D S
Sbjct: 551 PRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDAS 610

Query: 612 TSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQL 671
            +  S+P+ HGAGHIDP +A +PGL+YD+ PQ+Y  FLC+      Q+ VF K S  ++ 
Sbjct: 611 GAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHS--NRT 670

Query: 672 CEHKLS-NPGNLNYPSFAVVFDDEEVVK---YTRTVTNVGDETDAVYEVKVESPQGVEIS 731
           C+H L+ NPGNLNYP+ + +F +   VK     RTVTNVG    + Y+V V   +G  ++
Sbjct: 671 CKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISS-YKVSVSPFKGASVT 730

Query: 732 VVPNKLEFNEKKTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSF 761
           V P  L F  K    SY +TF      K    FG + W   +H VRSP+ +++
Sbjct: 731 VQPKTLNFTSKHQKLSYTVTFRTRFRMKR-PEFGGLVWKSTTHKVRSPVIITW 776