Lsi02G028090 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G028090
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionIsochorismatase domain-containing protein
Locationchr02: 34281258 .. 34282938 (-)
RNA-Seq ExpressionLsi02G028090
SyntenyLsi02G028090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGGGTTTGACTCCTCCCTTTTTTTTCTTCTATGTCTTCTTCTTCTCAGGCACTATGACTCCACATAAGCAGTTCCGACACGACCAATTTTGGTGAGGAAAAACGAGAGAGAGAGATGAGAGTGATTGTGAGTGAGGAAAGGAAGAGAGCAAGAGAGAAGAGAGCGAGATTGACTTTTTTGCGTGAACAAGTTTATTTCGGTATACAAACTTGACGTAAACAAATAGTCATCTAACATTTTTTTGAAGCAAATTTCCCTACAAATTTTGGTCCCCTGTACAATTATCCGGTCGTCGTACAGAACTGTTGTGTTGTGATAATTCTTCATCTTACAAGGATTTCATTATCAAGTCATCGCTATTTCCCCACAATTCCATTCCATCATCTTTCTCTTTCTACTGCGTTTTAGCCATGGCGGATCAATGGAAACGCACTGCTCTCCTTGTTATCGACATGCAGGTTTCCCCCCCCCCCCCTTATTTTCTTCCTAATTCAGTCCTCTTCTCTACTCCCACAATCAAACTCTTTCTTCCAATTCTTCATTACAGAAAGATTTCCTTGACGAACAATCCGTCTTCGCTGTACCAGGAGCCTACGCCATACTTCCAAGCCTCTCCAATGCTATCGAAATCGCAAGGAAGCGCGGCATGTTCATCGTTTGGGTCCGTTTAATTTTCTCTTCTACTTGAAAACGATAAAAGCCCTAATCTAATCCTCTGTTTGGATTGGATTTCAATAATGTTTGTTTTGCCCTAGGTTGTTCGAGAGCATGATCCTGAAGGACGAGATGTTGAACGCTTTCGCCGCCATTATTATGGCAGTGGGAAGCCGAATCCTGTTGTGAAGGAAAGCAAAGGAGCAGAATTAGTAGAAGGACTTGAAATCAAAGAAGGAGAATACAAATTGGTGAAAACAAGGTTCAGTGCCTTCTTTAATACCAATTTGCATTCCCTTCTACGAGGACAGGGTATTACTGATTTGGTTGTTTCTGGTAAAATTCCCTCTCTATATTCCAATGTGAATTTGTAGATTAAATGGTTGAATTGATGCATATTTTGTATATTAATGATTGTAGGTGTTCAAACCCCAAACTGTATCAGACAGACTGTTTTTGATGCTGTATCTTTGGATTATCATTCTATTACTGTTCTTTATGATGCATCTGCAGCTGCTACTCCCAAAATTCATCATGGTATTTCAAATTTCCTCCTCTATAGCTTTTGAGTTCTGTAATCAATAGATTGGCTATGATTTTGGTATTGAATCTGAAGACAAATGTTGTTCATGTAATGAATGATGAGGGATTTTTTTGAATTTGTTGTTTATCAGATAATATCAGTGACATGGAGAATGTGGGAGTTGTGGTGAAGAGAGTGGATGAATGGGGGAAGTCTAATGCTTGAATGAACCCAATTCTGCCATTGGAAATTATGAAGTTTCCAATTCTTATACATCTTGGTGTTGCTGGTAATTTTCAGCTCTTCTGCAATGAATTCATGGTTTCATGATCTTTAACCATATGCTTCTTGTGTGTAATTTGTGAAGACGTTCATACTCAAATAATCTCTCTCTCTCTCTTTGAACAACGACTTTATGTAATATAAATAAATACATATATTTAAACTTAGTTTTTATCATCTTAAACTTTTTAAAATAGTAAAATAAATTTATAAGGTTAATT

mRNA sequence

CAGGGTTTGACTCCTCCCTTTTTTTTCTTCTATGTCTTCTTCTTCTCAGGCACTATGACTCCACATAAGCAGTTCCGACACGACCAATTTTGGTGAGGAAAAACGAGAGAGAGAGATGAGAGTGATTGTGAGTGAGGAAAGGAAGAGAGCAAGAGAGAAGAGAGCGAGATTGACTTTTTTGCGTGAACAAGTTTATTTCGGTATACAAACTTGACGTAAACAAATAGTCATCTAACATTTTTTTGAAGCAAATTTCCCTACAAATTTTGGTCCCCTGTACAATTATCCGGTCGTCGTACAGAACTGTTGTGTTGTGATAATTCTTCATCTTACAAGGATTTCATTATCAAGTCATCGCTATTTCCCCACAATTCCATTCCATCATCTTTCTCTTTCTACTGCGTTTTAGCCATGGCGGATCAATGGAAACGCACTGCTCTCCTTGTTATCGACATGCAGAAAGATTTCCTTGACGAACAATCCGTCTTCGCTGTACCAGGAGCCTACGCCATACTTCCAAGCCTCTCCAATGCTATCGAAATCGCAAGGAAGCGCGGCATGTTCATCGTTTGGGTTGTTCGAGAGCATGATCCTGAAGGACGAGATGTTGAACGCTTTCGCCGCCATTATTATGGCAGTGGGAAGCCGAATCCTGTTGTGAAGGAAAGCAAAGGAGCAGAATTAGTAGAAGGACTTGAAATCAAAGAAGGAGAATACAAATTGGTGAAAACAAGGTTCAGTGCCTTCTTTAATACCAATTTGCATTCCCTTCTACGAGGACAGGGTATTACTGATTTGGTTGTTTCTGGTGTTCAAACCCCAAACTGTATCAGACAGACTGTTTTTGATGCTGTATCTTTGGATTATCATTCTATTACTGTTCTTTATGATGCATCTGCAGCTGCTACTCCCAAAATTCATCATGATAATATCAGTGACATGGAGAATGTGGGAGTTGTGGTGAAGAGAGTGGATGAATGGGGGAAGTCTAATGCTTGAATGAACCCAATTCTGCCATTGGAAATTATGAAGTTTCCAATTCTTATACATCTTGGTGTTGCTGGTAATTTTCAGCTCTTCTGCAATGAATTCATGGTTTCATGATCTTTAACCATATGCTTCTTGTGTGTAATTTGTGAAGACGTTCATACTCAAATAATCTCTCTCTCTCTCTTTGAACAACGACTTTATGTAATATAAATAAATACATATATTTAAACTTAGTTTTTATCATCTTAAACTTTTTAAAATAGTAAAATAAATTTATAAGGTTAATT

Coding sequence (CDS)

ATGGCGGATCAATGGAAACGCACTGCTCTCCTTGTTATCGACATGCAGAAAGATTTCCTTGACGAACAATCCGTCTTCGCTGTACCAGGAGCCTACGCCATACTTCCAAGCCTCTCCAATGCTATCGAAATCGCAAGGAAGCGCGGCATGTTCATCGTTTGGGTTGTTCGAGAGCATGATCCTGAAGGACGAGATGTTGAACGCTTTCGCCGCCATTATTATGGCAGTGGGAAGCCGAATCCTGTTGTGAAGGAAAGCAAAGGAGCAGAATTAGTAGAAGGACTTGAAATCAAAGAAGGAGAATACAAATTGGTGAAAACAAGGTTCAGTGCCTTCTTTAATACCAATTTGCATTCCCTTCTACGAGGACAGGGTATTACTGATTTGGTTGTTTCTGGTGTTCAAACCCCAAACTGTATCAGACAGACTGTTTTTGATGCTGTATCTTTGGATTATCATTCTATTACTGTTCTTTATGATGCATCTGCAGCTGCTACTCCCAAAATTCATCATGATAATATCAGTGACATGGAGAATGTGGGAGTTGTGGTGAAGAGAGTGGATGAATGGGGGAAGTCTAATGCTTGA

Protein sequence

MADQWKRTALLVIDMQKDFLDEQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHDPEGRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSLLRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDASAAATPKIHHDNISDMENVGVVVKRVDEWGKSNA
Homology
BLAST of Lsi02G028090 vs. ExPASy Swiss-Prot
Match: Q93Z51 (Probable inactive nicotinamidase At3g16190 OS=Arabidopsis thaliana OX=3702 GN=At3g16190 PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 9.4e-64
Identity = 117/196 (59.69%), Postives = 155/196 (79.08%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQKDFLDEQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHD 60
           MA++W+ TALLVIDMQ DF++E +V  V G  +I+P++   +E+AR+RG+ ++WVVREHD
Sbjct: 1   MAERWRNTALLVIDMQNDFIEEGAVTQVKGGKSIVPNVIRVVELARQRGILVIWVVREHD 60

Query: 61  PEGRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEI-KEGEYKLVKTRFSAFFNTNLHS 120
            +GRDVE FRRH Y S K  PV+K + GAELV+GL I +E +YK+VKTRFSAFF+TNLHS
Sbjct: 61  RQGRDVELFRRHNYSSEKVGPVIKGTVGAELVDGLMINEEDDYKIVKTRFSAFFSTNLHS 120

Query: 121 LLRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDASAAATPKIHHDNISDMEN 180
            L+  G+T LV++GVQTPNCIRQTVFDAV+LDY ++TV+ DA+AAATP+IH  NI DM+N
Sbjct: 121 FLQTSGVTKLVIAGVQTPNCIRQTVFDAVALDYPNVTVITDATAAATPEIHTANILDMKN 180

Query: 181 VGVVVKRVDEWGKSNA 196
           +GV    + EW +  A
Sbjct: 181 IGVKTPTLHEWSEELA 196

BLAST of Lsi02G028090 vs. ExPASy Swiss-Prot
Match: D5VGV1 (Ureidoacrylate amidohydrolase RutB OS=Caulobacter segnis (strain ATCC 21756 / DSM 7131 / JCM 7823 / NBRC 15250 / LMG 17158 / TK0059) OX=509190 GN=rutB PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 6.8e-14
Identity = 58/187 (31.02%), Postives = 90/187 (48.13%), Query Frame = 0

Query: 6   KRTALLVIDMQKDFLD-----EQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHD 65
           K TA++VIDMQ  +       + + F + GA  ++  +   +E+AR  GM +++     D
Sbjct: 27  KTTAVIVIDMQNAYASPGGYLDLAGFDISGAAKVIHEIKGVLEVARSAGMQVIYFQNGWD 86

Query: 66  PEGRDVERFRRHYYGSGKPN----------PVVKESKGA---------ELVEGLEIKEGE 125
            +  +         G G PN              E +G          ELV+ L+ + G+
Sbjct: 87  DQYVEAG-------GPGSPNWWKSNALKTMRAKPELQGKLLARGQWDYELVDELKPQPGD 146

Query: 126 YKLVKTRFSAFFNTNLHSLLRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDA 169
            +L KTR+S FFN+ L S+LR +GI  LV  G+ T  C+  T+ D   L+Y   TVL DA
Sbjct: 147 IQLHKTRYSGFFNSQLDSVLRARGIRHLVFVGIATNVCVESTLRDGFFLEYFG-TVLEDA 205

BLAST of Lsi02G028090 vs. ExPASy Swiss-Prot
Match: B7NLB5 (Ureidoacrylate amidohydrolase RutB OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) OX=585057 GN=rutB PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.5e-13
Identity = 57/189 (30.16%), Postives = 90/189 (47.62%), Query Frame = 0

Query: 6   KRTALLVIDMQKDFLDEQSVFAVPGAY------------AILPSLSNAIEIARKRGMFIV 65
           ++TAL+V+DM       Q+ +A PG Y             ++ ++  A+  AR  GM I+
Sbjct: 16  QQTALIVVDM-------QNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLII 75

Query: 66  WVVREHDPE--------------GRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKE 125
           W     D +                 ++  R+     GK   + K S   +LV+ L  + 
Sbjct: 76  WFQNGWDAQYVEAGGPGSPNFHKSNALKTMRKQPQLQGK--LLAKGSWDYQLVDELVPQP 135

Query: 126 GEYKLVKTRFSAFFNTNLHSLLRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLY 169
           G+  L K R+S+FFNT L S+LR +GI  LV +G+ T  C+  T+ D   L+Y  + VL 
Sbjct: 136 GDIVLPKPRYSSFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYFGV-VLE 194

BLAST of Lsi02G028090 vs. ExPASy Swiss-Prot
Match: B7MTF4 (Ureidoacrylate amidohydrolase RutB OS=Escherichia coli O81 (strain ED1a) OX=585397 GN=rutB PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.0e-13
Identity = 57/189 (30.16%), Postives = 89/189 (47.09%), Query Frame = 0

Query: 6   KRTALLVIDMQKDFLDEQSVFAVPGAY------------AILPSLSNAIEIARKRGMFIV 65
           ++TAL+V+DM       Q+ +A PG Y             ++ ++  A+  AR  GM I+
Sbjct: 16  QQTALIVVDM-------QNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLII 75

Query: 66  WVVREHDPE--------------GRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKE 125
           W     D +                 ++  R+     GK   + K S   +LV+ L  + 
Sbjct: 76  WFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGK--LLAKGSWDYQLVDELVPQP 135

Query: 126 GEYKLVKTRFSAFFNTNLHSLLRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLY 169
           G+  L K R+S FFNT L S+LR +GI  LV +G+ T  C+  T+ D   L+Y  + VL 
Sbjct: 136 GDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYFGV-VLE 194

BLAST of Lsi02G028090 vs. ExPASy Swiss-Prot
Match: C4ZQD9 (Ureidoacrylate amidohydrolase RutB OS=Escherichia coli (strain K12 / MC4100 / BW2952) OX=595496 GN=rutB PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.0e-13
Identity = 57/189 (30.16%), Postives = 89/189 (47.09%), Query Frame = 0

Query: 6   KRTALLVIDMQKDFLDEQSVFAVPGAY------------AILPSLSNAIEIARKRGMFIV 65
           +++AL+V+DM       Q+ +A PG Y             ++ ++  A+  AR  GM I+
Sbjct: 16  QQSALIVVDM-------QNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLII 75

Query: 66  WVVREHDPE--------------GRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKE 125
           W     D +                 ++  R+     GK   + K S   +LV+ L  + 
Sbjct: 76  WFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGK--LLAKGSWDYQLVDELVPQP 135

Query: 126 GEYKLVKTRFSAFFNTNLHSLLRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLY 169
           G+  L K R+S FFNT L S+LR +GI  LV +G+ T  C+  T+ D   L+Y  + VL 
Sbjct: 136 GDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYFGV-VLE 194

BLAST of Lsi02G028090 vs. ExPASy TrEMBL
Match: A0A0A0K7E5 (Isochorismatase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447030 PE=3 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 2.5e-91
Identity = 166/192 (86.46%), Postives = 178/192 (92.71%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQKDFLDEQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHD 60
           M DQWKRTALLVIDMQ DF DE S FAVPGA  I+PS+SNAIEIAR RG+FI+WVVREHD
Sbjct: 1   MTDQWKRTALLVIDMQTDFFDEHSAFAVPGASTIIPSVSNAIEIARNRGLFIIWVVREHD 60

Query: 61  PEGRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
            EGRDVERFRRHYYG+GKPNP VK SKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPSVKGSKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120

Query: 121 LRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDASAAATPKIHHDNISDMENV 180
           L+G GITDLVV GVQTPNCIRQTVFDAV+LDYHSIT+LYDA+AAATPKIHHDN +DMENV
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVFDAVALDYHSITLLYDATAAATPKIHHDNTTDMENV 180

Query: 181 GVVVKRVDEWGK 193
           GVVVKRVD+WG+
Sbjct: 181 GVVVKRVDQWGE 192

BLAST of Lsi02G028090 vs. ExPASy TrEMBL
Match: A0A1S3CP26 (probable inactive nicotinamidase At3g16190 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503139 PE=3 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.2e-91
Identity = 164/195 (84.10%), Postives = 183/195 (93.85%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQKDFLDEQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHD 60
           MADQWKRTALLVIDMQ+DF+DE SVFAVPGA  I+PS+S A+EIAR RG+FI+WVVREHD
Sbjct: 1   MADQWKRTALLVIDMQRDFIDEHSVFAVPGASTIIPSVSTAVEIARNRGLFIIWVVREHD 60

Query: 61  PEGRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
            EGRDVERFRRHYYG+GKPNP+VK SKGAELV+GLEIKEGEYKLVKTRFSAFFNTNL SL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLRSL 120

Query: 121 LRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDASAAATPKIHHDNISDMENV 180
           L+G GITDLVV GVQTPNCIRQTVFDAV+LDYHSIT+LYDA+AAATPK+HHDNI+DM NV
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVFDAVALDYHSITLLYDATAAATPKVHHDNITDMVNV 180

Query: 181 GVVVKRVDEWGKSNA 196
           GV VKRVDEWG+S++
Sbjct: 181 GVEVKRVDEWGESDS 195

BLAST of Lsi02G028090 vs. ExPASy TrEMBL
Match: A0A5A7UTR3 (Putative inactive nicotinamidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G00370 PE=3 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 9.3e-91
Identity = 163/195 (83.59%), Postives = 183/195 (93.85%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQKDFLDEQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHD 60
           MADQWKRTALLVIDMQ+DF+DE SVFAVPGA  I+PS+S A+EIAR RG+FI+WVVREHD
Sbjct: 1   MADQWKRTALLVIDMQRDFIDEHSVFAVPGASTIIPSVSTAVEIARNRGLFIIWVVREHD 60

Query: 61  PEGRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
            EGRDVERFRRHYYG+GKPNP+VK SKGAELV+GLEIKEGEYKLVKTRFSAFFNTNL SL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLRSL 120

Query: 121 LRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDASAAATPKIHHDNISDMENV 180
           L+G GITDLVV GVQTPNCIRQTV+DAV+LDYHSIT+LYDA+AAATPK+HHDNI+DM NV
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVYDAVALDYHSITLLYDATAAATPKVHHDNITDMVNV 180

Query: 181 GVVVKRVDEWGKSNA 196
           GV VKRVDEWG+S++
Sbjct: 181 GVEVKRVDEWGESDS 195

BLAST of Lsi02G028090 vs. ExPASy TrEMBL
Match: A0A6J1DJ62 (probable inactive nicotinamidase At3g16190 OS=Momordica charantia OX=3673 GN=LOC111021389 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.7e-87
Identity = 157/194 (80.93%), Postives = 180/194 (92.78%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQKDFLDEQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHD 60
           MA+QW  TALLVIDMQ+DF DE+SV A+PGAYAI+PS+ +A+EIARKR M IVWVVREHD
Sbjct: 1   MANQWNHTALLVIDMQRDFFDERSVIAIPGAYAIVPSVVDAVEIARKRRMLIVWVVREHD 60

Query: 61  PEGRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
           PEGRDVERFRRH+YGSGK NPV K S+GAELV+GLEIKEGEYKLVKTRFSAFFNTNLHSL
Sbjct: 61  PEGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEIKEGEYKLVKTRFSAFFNTNLHSL 120

Query: 121 LRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDASAAATPKIHHDNISDMENV 180
           L+ +GI +LVV+GVQTPNCIRQTVFDAV+LDYHSIT+L+DA+AAATPK HHDNI+DMENV
Sbjct: 121 LQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITLLHDATAAATPKTHHDNIADMENV 180

Query: 181 GVVVKRVDEWGKSN 195
           GVV KRVD+WG+S+
Sbjct: 181 GVVAKRVDQWGESD 194

BLAST of Lsi02G028090 vs. ExPASy TrEMBL
Match: A0A5D3BXX3 (Putative inactive nicotinamidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002130 PE=3 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.4e-86
Identity = 163/222 (73.42%), Postives = 183/222 (82.43%), Query Frame = 0

Query: 1   MADQWKRTALLVIDM---------------------------QKDFLDEQSVFAVPGAYA 60
           MADQWKRTALLVIDM                           Q+DF+DE SVFAVPGA  
Sbjct: 1   MADQWKRTALLVIDMQVXPFYFLPNSIPFSTPTIILFNFFQLQRDFIDEHSVFAVPGAST 60

Query: 61  ILPSLSNAIEIARKRGMFIVWVVREHDPEGRDVERFRRHYYGSGKPNPVVKESKGAELVE 120
           I+PS+S A+EIAR RG+FI+WVVREHD EGRDVERFRRHYYG+GKPNP+VK SKGAELV+
Sbjct: 61  IIPSVSTAVEIARNRGLFIIWVVREHDGEGRDVERFRRHYYGNGKPNPLVKGSKGAELVQ 120

Query: 121 GLEIKEGEYKLVKTRFSAFFNTNLHSLLRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYH 180
           GLEIKEGEYKLVKTRFSAFFNTNL SLL+G GITDLVV GVQTPNCIRQTV+DAV+LDYH
Sbjct: 121 GLEIKEGEYKLVKTRFSAFFNTNLRSLLQGAGITDLVVCGVQTPNCIRQTVYDAVALDYH 180

Query: 181 SITVLYDASAAATPKIHHDNISDMENVGVVVKRVDEWGKSNA 196
           SIT+LYDA+AAATPK+HHDNI+DM NVGV VKRVDEWG+S++
Sbjct: 181 SITLLYDATAAATPKVHHDNITDMVNVGVEVKRVDEWGESDS 222

BLAST of Lsi02G028090 vs. NCBI nr
Match: XP_004141764.1 (probable inactive nicotinamidase At3g16190 [Cucumis sativus] >KGN45388.1 hypothetical protein Csa_015610 [Cucumis sativus])

HSP 1 Score: 344.7 bits (883), Expect = 5.1e-91
Identity = 166/192 (86.46%), Postives = 178/192 (92.71%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQKDFLDEQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHD 60
           M DQWKRTALLVIDMQ DF DE S FAVPGA  I+PS+SNAIEIAR RG+FI+WVVREHD
Sbjct: 1   MTDQWKRTALLVIDMQTDFFDEHSAFAVPGASTIIPSVSNAIEIARNRGLFIIWVVREHD 60

Query: 61  PEGRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
            EGRDVERFRRHYYG+GKPNP VK SKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPSVKGSKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120

Query: 121 LRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDASAAATPKIHHDNISDMENV 180
           L+G GITDLVV GVQTPNCIRQTVFDAV+LDYHSIT+LYDA+AAATPKIHHDN +DMENV
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVFDAVALDYHSITLLYDATAAATPKIHHDNTTDMENV 180

Query: 181 GVVVKRVDEWGK 193
           GVVVKRVD+WG+
Sbjct: 181 GVVVKRVDQWGE 192

BLAST of Lsi02G028090 vs. NCBI nr
Match: XP_008465520.1 (PREDICTED: probable inactive nicotinamidase At3g16190 isoform X1 [Cucumis melo])

HSP 1 Score: 344.0 bits (881), Expect = 8.6e-91
Identity = 164/195 (84.10%), Postives = 183/195 (93.85%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQKDFLDEQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHD 60
           MADQWKRTALLVIDMQ+DF+DE SVFAVPGA  I+PS+S A+EIAR RG+FI+WVVREHD
Sbjct: 1   MADQWKRTALLVIDMQRDFIDEHSVFAVPGASTIIPSVSTAVEIARNRGLFIIWVVREHD 60

Query: 61  PEGRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
            EGRDVERFRRHYYG+GKPNP+VK SKGAELV+GLEIKEGEYKLVKTRFSAFFNTNL SL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLRSL 120

Query: 121 LRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDASAAATPKIHHDNISDMENV 180
           L+G GITDLVV GVQTPNCIRQTVFDAV+LDYHSIT+LYDA+AAATPK+HHDNI+DM NV
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVFDAVALDYHSITLLYDATAAATPKVHHDNITDMVNV 180

Query: 181 GVVVKRVDEWGKSNA 196
           GV VKRVDEWG+S++
Sbjct: 181 GVEVKRVDEWGESDS 195

BLAST of Lsi02G028090 vs. NCBI nr
Match: KAA0059313.1 (putative inactive nicotinamidase [Cucumis melo var. makuwa])

HSP 1 Score: 342.8 bits (878), Expect = 1.9e-90
Identity = 163/195 (83.59%), Postives = 183/195 (93.85%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQKDFLDEQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHD 60
           MADQWKRTALLVIDMQ+DF+DE SVFAVPGA  I+PS+S A+EIAR RG+FI+WVVREHD
Sbjct: 1   MADQWKRTALLVIDMQRDFIDEHSVFAVPGASTIIPSVSTAVEIARNRGLFIIWVVREHD 60

Query: 61  PEGRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
            EGRDVERFRRHYYG+GKPNP+VK SKGAELV+GLEIKEGEYKLVKTRFSAFFNTNL SL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLRSL 120

Query: 121 LRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDASAAATPKIHHDNISDMENV 180
           L+G GITDLVV GVQTPNCIRQTV+DAV+LDYHSIT+LYDA+AAATPK+HHDNI+DM NV
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVYDAVALDYHSITLLYDATAAATPKVHHDNITDMVNV 180

Query: 181 GVVVKRVDEWGKSNA 196
           GV VKRVDEWG+S++
Sbjct: 181 GVEVKRVDEWGESDS 195

BLAST of Lsi02G028090 vs. NCBI nr
Match: XP_038898995.1 (probable inactive nicotinamidase At3g16190 [Benincasa hispida])

HSP 1 Score: 342.4 bits (877), Expect = 2.5e-90
Identity = 168/191 (87.96%), Postives = 178/191 (93.19%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQKDFLDEQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHD 60
           MADQ K TALLVIDMQKDFLDEQSVFAVPGA  ILPS+ N +EIAR RG+FIVWVVREHD
Sbjct: 1   MADQLKHTALLVIDMQKDFLDEQSVFAVPGATTILPSVINTVEIARNRGLFIVWVVREHD 60

Query: 61  PEGRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
           P+GRDVERFRRHYYGSGKPNPV K SKGAELVEGLE+KEGEYKLVKTRFSAFFNTNLHSL
Sbjct: 61  PQGRDVERFRRHYYGSGKPNPVSKGSKGAELVEGLEMKEGEYKLVKTRFSAFFNTNLHSL 120

Query: 121 LRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDASAAATPKIHHDNISDMENV 180
           L+G GITDLVVSGVQTPNCIRQTVFDAV+LDYHSIT+L DA+AAATPKIHH NI+DMENV
Sbjct: 121 LQGMGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLSDATAAATPKIHHGNITDMENV 180

Query: 181 GVVVKRVDEWG 192
           GVVV RVDEWG
Sbjct: 181 GVVVMRVDEWG 191

BLAST of Lsi02G028090 vs. NCBI nr
Match: XP_022154038.1 (probable inactive nicotinamidase At3g16190 [Momordica charantia])

HSP 1 Score: 330.9 bits (847), Expect = 7.6e-87
Identity = 157/194 (80.93%), Postives = 180/194 (92.78%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQKDFLDEQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHD 60
           MA+QW  TALLVIDMQ+DF DE+SV A+PGAYAI+PS+ +A+EIARKR M IVWVVREHD
Sbjct: 1   MANQWNHTALLVIDMQRDFFDERSVIAIPGAYAIVPSVVDAVEIARKRRMLIVWVVREHD 60

Query: 61  PEGRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
           PEGRDVERFRRH+YGSGK NPV K S+GAELV+GLEIKEGEYKLVKTRFSAFFNTNLHSL
Sbjct: 61  PEGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEIKEGEYKLVKTRFSAFFNTNLHSL 120

Query: 121 LRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDASAAATPKIHHDNISDMENV 180
           L+ +GI +LVV+GVQTPNCIRQTVFDAV+LDYHSIT+L+DA+AAATPK HHDNI+DMENV
Sbjct: 121 LQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITLLHDATAAATPKTHHDNIADMENV 180

Query: 181 GVVVKRVDEWGKSN 195
           GVV KRVD+WG+S+
Sbjct: 181 GVVAKRVDQWGESD 194

BLAST of Lsi02G028090 vs. TAIR 10
Match: AT3G16190.1 (Isochorismatase family protein )

HSP 1 Score: 244.6 bits (623), Expect = 6.6e-65
Identity = 117/196 (59.69%), Postives = 155/196 (79.08%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQKDFLDEQSVFAVPGAYAILPSLSNAIEIARKRGMFIVWVVREHD 60
           MA++W+ TALLVIDMQ DF++E +V  V G  +I+P++   +E+AR+RG+ ++WVVREHD
Sbjct: 1   MAERWRNTALLVIDMQNDFIEEGAVTQVKGGKSIVPNVIRVVELARQRGILVIWVVREHD 60

Query: 61  PEGRDVERFRRHYYGSGKPNPVVKESKGAELVEGLEI-KEGEYKLVKTRFSAFFNTNLHS 120
            +GRDVE FRRH Y S K  PV+K + GAELV+GL I +E +YK+VKTRFSAFF+TNLHS
Sbjct: 61  RQGRDVELFRRHNYSSEKVGPVIKGTVGAELVDGLMINEEDDYKIVKTRFSAFFSTNLHS 120

Query: 121 LLRGQGITDLVVSGVQTPNCIRQTVFDAVSLDYHSITVLYDASAAATPKIHHDNISDMEN 180
            L+  G+T LV++GVQTPNCIRQTVFDAV+LDY ++TV+ DA+AAATP+IH  NI DM+N
Sbjct: 121 FLQTSGVTKLVIAGVQTPNCIRQTVFDAVALDYPNVTVITDATAAATPEIHTANILDMKN 180

Query: 181 VGVVVKRVDEWGKSNA 196
           +GV    + EW +  A
Sbjct: 181 IGVKTPTLHEWSEELA 196

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z519.4e-6459.69Probable inactive nicotinamidase At3g16190 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
D5VGV16.8e-1431.02Ureidoacrylate amidohydrolase RutB OS=Caulobacter segnis (strain ATCC 21756 / DS... [more]
B7NLB51.5e-1330.16Ureidoacrylate amidohydrolase RutB OS=Escherichia coli O7:K1 (strain IAI39 / ExP... [more]
B7MTF42.0e-1330.16Ureidoacrylate amidohydrolase RutB OS=Escherichia coli O81 (strain ED1a) OX=5853... [more]
C4ZQD92.0e-1330.16Ureidoacrylate amidohydrolase RutB OS=Escherichia coli (strain K12 / MC4100 / BW... [more]
Match NameE-valueIdentityDescription
A0A0A0K7E52.5e-9186.46Isochorismatase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G44... [more]
A0A1S3CP264.2e-9184.10probable inactive nicotinamidase At3g16190 isoform X1 OS=Cucumis melo OX=3656 GN... [more]
A0A5A7UTR39.3e-9183.59Putative inactive nicotinamidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1DJ623.7e-8780.93probable inactive nicotinamidase At3g16190 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A5D3BXX32.4e-8673.42Putative inactive nicotinamidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
XP_004141764.15.1e-9186.46probable inactive nicotinamidase At3g16190 [Cucumis sativus] >KGN45388.1 hypothe... [more]
XP_008465520.18.6e-9184.10PREDICTED: probable inactive nicotinamidase At3g16190 isoform X1 [Cucumis melo][more]
KAA0059313.11.9e-9083.59putative inactive nicotinamidase [Cucumis melo var. makuwa][more]
XP_038898995.12.5e-9087.96probable inactive nicotinamidase At3g16190 [Benincasa hispida][more]
XP_022154038.17.6e-8780.93probable inactive nicotinamidase At3g16190 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G16190.16.6e-6559.69Isochorismatase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036380Isochorismatase-like superfamilyGENE3D3.40.50.850coord: 2..194
e-value: 4.1E-53
score: 182.2
IPR036380Isochorismatase-like superfamilySUPERFAMILY52499Isochorismatase-like hydrolasescoord: 5..190
IPR000868Isochorismatase-likePFAMPF00857Isochorismatasecoord: 8..184
e-value: 4.9E-37
score: 127.8
NoneNo IPR availablePANTHERPTHR47044OS02G0276400 PROTEINcoord: 1..193
NoneNo IPR availableCDDcd00431cysteine_hydrolasescoord: 9..178
e-value: 2.44313E-44
score: 143.178

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G028090.1Lsi02G028090.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane