Lsi02G027620 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G027620
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Acyl-[acyl-carrier-protein] hydrolase
Location	chr02: 33886687 .. 33889710 (-)
RNA-Seq Expression	Lsi02G027620
Synteny	Lsi02G027620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTAGGTTCATAGCTAGCTGTGGTGATCCGGTTTGTAGTTTGTTGAAGATCTTGTTTTGCTCTCTTTTTTCTTTTTCTTTTTCTCCCTTTTATTTGTTTTCTCTTTACCCATTTCTACTTTGCCCAATTCTCTGATATGTATTCCTTCTGCAAATGTGTATAACTTCAGTTCCTAATTGGATTTGGAGGGTCGACATTTTCAGTTGCTCACAATTTTTGTAGATTCTAGACAAGGAAGTTATATGGGTTTTGCTCCAATCCAATATCTGAGGTTATATTTAGGTGAAGAGAGCTAGTTTTCTTTCCCTTTCTCCATATCCTCCATGGTTATACATCATCCTCTGCTACATACACATACTCAACTTAAGATGTACTTTGTCGATTTCACTGATTGCGTTGTTATGTATAAACACCATATATGGAAATTTTTGCGATGGAATTACTGATCTACTTTTATGTATTCGATTAATTTTTTTTATTTATAGAAATATTTGTTGATATTGATATGTTCTTAAAGTTAGAATTTTTGCTATGTACATCAACATATCAGATTTTTCAATTCTTGCCCATAACCAAACTACATATTAGTTACCCAACTCTTGAACTTGAGTAAATGTAGTAAACTAAACTTTAACTAATACATGTATTGGCATTCATGCTGCAAAGATATTTTAATATTTTTTAACCAAAAAAAATTATTATTATTTTGATATTAACTATTAACTTTTGAAATGTTAGTTGGTGTTTTATCTATAAAGTTTTACCCAATTTACAAAATATATTATATATTTATTTATTTATTGAGTTCAACAGTATGTTGGAATAATTAAATTTTTAACCTCTTAGTAAAGTACTAGTTGCATTATGCTTATATTGAGTTACAAATTTCTATGATATCATTAATATTTGATTTTATTCTTTGTTTGAATCAAGTCGATGTTAAAGGATGGTATCTAAATTTGTTGTTAATTAGATGGAGAATGAATTTTTGTAGTAGTTGAAGTTGCTTCACCAAGTTGCCCATGGGTAAATGAAGCTACCAATTGTCCCAAACAATAAATTATTAGATGAAATAATATAAATAAGAATTTTTTTTGGAAGGTGAAACAGATTTTTGACTTCAAAATTAATAGTGCATTCTTTATGTCATTTGAAATGTACTCATTTTGGTAATATATACAAGCTTTTAAAATTCGTGAAATCATTACTTAGGAAGAAAATTATGAGACATCTAAAATAATTAAGATAAACAGAACAATCAATATAATATCTTTCCAATGTCATGCAATAGCAACACATTGGCTGGCAAATTCCAAACCAACAAAAGTTTCTTTAACTTTATATAATAAAAAATCTCAAATGCTTGTCCAACAGAAGAAGAAAATAAAAAATTATAATAGACATCTTTGCTATTCTTGTCTGCATAAATTTTGTCTTTTCTCATACTATATCTAGAATCTTGTTTCAAAAGAATATAGAAACTAAAATTAAACCAACTTTAAAGTATCGAGACAAAAAATACATTTTAACCTTAATTTTACTTAGACTCTGTTTGATAATGATTCCATTTCTAGTTTCCTATTTCTAAAAGTTATGTTATTTTCTCTCAATCTCTTTATTATGGATTTGACTTTCCATAAAAAAAAAAAAAGATTTAAATTTTCAGTCTAAGTTTCAAAAATAAAAAATGGTTTGTTTTTATTATTTTTTAAGTTATAAAAATTTTGTTTAGAATTTGAAAACATTATGAGACTTGTATTTTGTTATTAGAGGTAGTTAATTAGTTGGTTCTAGTTGGCTGCTTGGTCAGAAGTTGCAGCCTATACATATGCAAACTGCTTTGTATTTGATGACACTTTTTTTGTCATTTAAATGTAAGTGCTTCCCTTGTTTACATCAAATTACATAGAGAGTTAGAATTTAAAAGAATAAGAAAATAAAACAAAACAATTCTGAATTGATTGAGATACAAAACTCAACATCAACTTATAAAAACAATGAATGTCTTCTAACATCTTAAAACTGAAAATTCATGAAAACAACATTAATGGTAATGGTAAAGGTTTAAATTAGTAGAACCATATAAAAAGAAAAAAAAACTGAAAAATTAAATTTCACTAAAGAACTCAAAAAAATTCTTATAAATGGCAATTATCTTCTCTCTTCCAATGCTCCATTTTCTATCATCAAAACTTTTTTGCAAAAGTACCCTATGATGGCAGCAATATCTTCATCTTATAGTTGTAATTTTGGTAAGAAAATGAATTCAATTTTGAAGAAAGAACAAAGATTTGGAGATACAAGTAGAGGCAATTTGCAGTTCAAAGGCCATCCCAAAATTTGCTTAGAAAGGCTCAAAACTTCTAACATTATTGCTAAGTCCACCATTGCAAGTAAGATTAAAAATTCTTTCTTTTTTCCAACACTTTTGTTTGTGTTTTTTTGAAAGATATAATTGATATATATTTTTGGCAGGTGAGGTTAGAAAGATTGATAGTTATGAGTCTAGAAGTCTTGATATAACTCATGGGAACTTAACGATGGGAGAATTAGTGAACGATGGTTTGGTTTATCAACGCCATTTCTTGGTCAAATCCTACGAGTCGGGACCAGATAAAAATATGACAATGTTTTTCATCATCAACCAGTTACAGGTTAGAAATTGTAACAGTTGTAGGTTTAATATTTATGTTTTGAGGATTCAAATTTTACCACTATTGTTAGTTTGAGGGTCCAATTTTAACACTTTGTAAGTTTGAGAGTGCAATTTTTACTACCACCATACTTTAGGATTTTTTTTTTTTTTTTTTTGCAATTTATACTTTATTTTTATTTATTAATTTATTTATTGGTATTAATTTAATTTATCCTTTATTTTCATTAGGAGTCTAAACTAAACCACTTGAAAAGTTGTGGATTGGTGACGACAGGATTGGGCACAACACCCGAAATGACCAAAAGGGGCCTCCTATGGGTGGTTCATAAATTGCAGGTGGTGGTGGACCAATATCCTTCTTGGTAA

mRNA sequence

ATGCTAGCAATATCTTCATCTTATAGTTGTAATTTTGGTAAGAAAATGAATTCAATTTTGAAGAAAGAACAAAGATTTGGAGATACAAGTAGAGGCAATTTGCAGTTCAAAGGCCATCCCAAAATTTGCTTAGAAAGGCTCAAAACTTCTAACATTATTGCTAAGTCCACCATTGCAAGTGAGGTTAGAAAGATTGATAGTTATGAGTCTAGAAGTCTTGATATAACTCATGGGAACTTAACGATGGGAGAATTAGTGAACGATGGTTTGGTTTATCAACGCCATTTCTTGGAGTCTAAACTAAACCACTTGAAAAGTTGTGGATTGGTGACGACAGGATTGGGCACAACACCCGAAATGACCAAAAGGGGCCTCCTATGGGTGGTTCATAAATTGCAGGTGGTGGTGGACCAATATCCTTCTTGGTAA

Coding sequence (CDS)

ATGCTAGCAATATCTTCATCTTATAGTTGTAATTTTGGTAAGAAAATGAATTCAATTTTGAAGAAAGAACAAAGATTTGGAGATACAAGTAGAGGCAATTTGCAGTTCAAAGGCCATCCCAAAATTTGCTTAGAAAGGCTCAAAACTTCTAACATTATTGCTAAGTCCACCATTGCAAGTGAGGTTAGAAAGATTGATAGTTATGAGTCTAGAAGTCTTGATATAACTCATGGGAACTTAACGATGGGAGAATTAGTGAACGATGGTTTGGTTTATCAACGCCATTTCTTGGAGTCTAAACTAAACCACTTGAAAAGTTGTGGATTGGTGACGACAGGATTGGGCACAACACCCGAAATGACCAAAAGGGGCCTCCTATGGGTGGTTCATAAATTGCAGGTGGTGGTGGACCAATATCCTTCTTGGTAA

Protein sequence

MLAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIASEVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFLESKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW

Homology

[Close]

BLAST of Lsi02G027620 vs. ExPASy Swiss-Prot
Match: Q9SJE2 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FATB PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.0e-11
Identity = 35/97 (36.08%), Postives = 54/97 (55.67%), Query Frame = 0


Query: 68  YESRSLDITHGNLTMGELVNDGLVYQR----------------------HFLESKLNHLK 127
           ++ R  D+      +G +V DGLV+++                      H  E+ LNH+K
Sbjct: 115 WKPRRSDMLVDPFGIGRIVQDGLVFRQNFSIRSYEIGADRSASIETVMNHLQETALNHVK 174

Query: 128 SCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           + GL+  G G+TPEM K+ L+WVV ++QVVVD+YP+W
Sbjct: 175 TAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTW 211

[Close]

BLAST of Lsi02G027620 vs. ExPASy Swiss-Prot
Match: G3ESV0 (Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea viscosissima OX=857185 GN=FATB2 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.8e-11
Identity = 35/94 (37.23%), Postives = 51/94 (54.26%), Query Frame = 0


Query: 71  RSLDITHGNLTMGELVNDGLVYQR----------------------HFLESKLNHLKSCG 130
           + LD+      +G +V DGLV+++                      H  E+ LNH+K+ G
Sbjct: 114 KRLDMLEDPFGLGRVVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKTAG 173

Query: 131 LVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           L   G G TPEM KR L+WVV K+QV+V++YP+W
Sbjct: 174 LSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTW 207

[Close]

BLAST of Lsi02G027620 vs. ExPASy Swiss-Prot
Match: Q9SQI3 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum OX=3635 GN=FATB1 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.0e-11
Identity = 34/97 (35.05%), Postives = 52/97 (53.61%), Query Frame = 0


Query: 68  YESRSLDITHGNLTMGELVNDGLVYQR----------------------HFLESKLNHLK 127
           ++ R  D+      +G++V DGLV+ +                      H  E+ +NH +
Sbjct: 110 WKPRRPDMVIDPFGIGKIVQDGLVFSQNFSIRSYEIGADQTASIETLMNHLQETAINHCR 169

Query: 128 SCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           S GL+  G G TPEM K+ L+WVV ++QVVVD+YP+W
Sbjct: 170 SAGLLGEGFGATPEMCKKNLIWVVTRMQVVVDRYPTW 206

[Close]

BLAST of Lsi02G027620 vs. ExPASy Swiss-Prot
Match: Q39513 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana OX=36775 GN=FATB1 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.9e-10
Identity = 33/83 (39.76%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 82  MGELVNDGLVYQR----------------------HFLESKLNHLKSCGLVTTGLGTTPE 141
           +G +V DGLV+++                      H  E+ LNH+K  GL   G G TPE
Sbjct: 128 LGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGRTPE 187

Query: 142 MTKRGLLWVVHKLQVVVDQYPSW 143
           M KR L+WVV K+QV+V++YP+W
Sbjct: 188 MYKRDLIWVVAKMQVMVNRYPTW 210

[Close]

BLAST of Lsi02G027620 vs. ExPASy Swiss-Prot
Match: G3ESV1 (Acyl-[acyl-carrier-protein] hydrolase FATB3, chloroplastic OS=Cuphea viscosissima OX=857185 GN=FATB3 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 7.4e-10
Identity = 27/47 (57.45%), Postives = 35/47 (74.47%), Query Frame = 0


Query: 96  FLESKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           F E+ LNH KS GL+  G G TPEM KR L+WVV K+Q+ V++YP+W
Sbjct: 161 FQETSLNHCKSLGLLNDGFGRTPEMCKRDLIWVVTKMQIEVNRYPTW 207

[Close]

BLAST of Lsi02G027620 vs. ExPASy TrEMBL
Match: A0A0A0K767 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis sativus OX=3659 GN=Csa_7G440570 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 5.6e-61
Identity = 123/164 (75.00%), Postives = 133/164 (81.10%), Query Frame = 0


Query: 1   MLAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIAS 60
           M AISSSY CNFG++ NS LKKEQRFGD++RGNLQ K HPK CLE L+TSNIIAKS+IAS
Sbjct: 1   MAAISSSYCCNFGEEFNSNLKKEQRFGDSNRGNLQLKNHPKFCLEGLRTSNIIAKSSIAS 60

Query: 61  EVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFL----------------------E 120
           EVRKI+SYESR+LDITHGNLTMGEL+NDGLVYQRHFL                      E
Sbjct: 61  EVRKINSYESRNLDITHGNLTMGELLNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQE 120

Query: 121 SKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           SKLNHLKSCGLVTTGLGTTP MTKRGL+WVVHKLQVVVDQYPSW
Sbjct: 121 SKLNHLKSCGLVTTGLGTTPGMTKRGLIWVVHKLQVVVDQYPSW 164

[Close]

BLAST of Lsi02G027620 vs. ExPASy TrEMBL
Match: A0A5A7UZ85 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002510 PE=3 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 3.6e-60
Identity = 122/164 (74.39%), Postives = 132/164 (80.49%), Query Frame = 0


Query: 1   MLAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIAS 60
           M A+SSSY CN GKK NS LKKEQRFGD++ GNLQ K HPK+ LE L+TSNIIAKS+IAS
Sbjct: 1   MAAVSSSYCCNLGKKFNSNLKKEQRFGDSNTGNLQLKNHPKLFLEGLRTSNIIAKSSIAS 60

Query: 61  EVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFL----------------------E 120
           EVRKI+SYESR+LDITHGNLTMGEL+NDGLVYQRHFL                      E
Sbjct: 61  EVRKINSYESRNLDITHGNLTMGELLNDGLVYQRHFLVKSYESGPDKKMTMFFIINQLHE 120

Query: 121 SKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           SKLNHLKSCGLVTTGLGTTPEMTKRGL+WVVHKLQVVVDQYPSW
Sbjct: 121 SKLNHLKSCGLVTTGLGTTPEMTKRGLIWVVHKLQVVVDQYPSW 164

[Close]

BLAST of Lsi02G027620 vs. ExPASy TrEMBL
Match: A0A6J1KZF4 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111497736 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 8.5e-25
Identity = 74/152 (48.68%), Postives = 86/152 (56.58%), Query Frame = 0


Query: 18  SILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIASEVRKIDSYESRSLDITH 77
           SI  +E  FG  +  +L  KG PK     LK+S+I     IAS  + +D Y SR  +IT 
Sbjct: 5   SISMREPTFGHATTPSLHLKGRPKSASLTLKSSHI--SQPIASGFKSMDDYVSRKPEITR 64

Query: 78  GNLTMGELVNDGLVYQRHFL----------------------ESKLNHLKSCGLVTTGL- 137
            NL + E +NDGLVYQ HFL                      ESKLNH  SCGLV TGL 
Sbjct: 65  ENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVKTGLG 124

Query: 138 ----GTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
               GTT EMTKR L+WVVHKLQVVVDQ+PSW
Sbjct: 125 KTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSW 154

[Close]

BLAST of Lsi02G027620 vs. ExPASy TrEMBL
Match: A0A6J1H8Q2 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461080 PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 4.6e-23
Identity = 71/152 (46.71%), Postives = 82/152 (53.95%), Query Frame = 0


Query: 18  SILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIASEVRKIDSYESRSLDITH 77
           SI  +E  FG  +  +L  KG PK     LK+S+I      A     +D Y SR  +IT 
Sbjct: 5   SISMREPTFGHATTPSLHLKGRPKSASLTLKSSHI--SQPTAGGFESMDDYMSRKPEITR 64

Query: 78  GNLTMGELVNDGLVYQRHFL----------------------ESKLNHLKSCGLV----- 137
            NL + E +NDGLVYQ HFL                      ESKLNH  SCGLV     
Sbjct: 65  ENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERGLG 124

Query: 138 TTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
            TG GTT EMTKR L+WVVHKLQVVVDQ+PSW
Sbjct: 125 KTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSW 154

[Close]

BLAST of Lsi02G027620 vs. ExPASy TrEMBL
Match: A0A6J1CMR0 (Acyl-[acyl-carrier-protein] hydrolase OS=Momordica charantia OX=3673 GN=LOC111012889 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 5.3e-11
Identity = 34/45 (75.56%), Postives = 37/45 (82.22%), Query Frame = 0


Query: 98  ESKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           ES +NH KSCGLV TG  +T EM KRGL+WVVHKLQVVVD YPSW
Sbjct: 10  ESAINHFKSCGLVETGYASTREMDKRGLIWVVHKLQVVVDLYPSW 54

[Close]

BLAST of Lsi02G027620 vs. NCBI nr
Match: KGN45333.1 (hypothetical protein Csa_015751 [Cucumis sativus])

HSP 1 Score: 243.4 bits (620), Expect = 1.2e-60
Identity = 123/164 (75.00%), Postives = 133/164 (81.10%), Query Frame = 0


Query: 1   MLAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIAS 60
           M AISSSY CNFG++ NS LKKEQRFGD++RGNLQ K HPK CLE L+TSNIIAKS+IAS
Sbjct: 1   MAAISSSYCCNFGEEFNSNLKKEQRFGDSNRGNLQLKNHPKFCLEGLRTSNIIAKSSIAS 60

Query: 61  EVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFL----------------------E 120
           EVRKI+SYESR+LDITHGNLTMGEL+NDGLVYQRHFL                      E
Sbjct: 61  EVRKINSYESRNLDITHGNLTMGELLNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQE 120

Query: 121 SKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           SKLNHLKSCGLVTTGLGTTP MTKRGL+WVVHKLQVVVDQYPSW
Sbjct: 121 SKLNHLKSCGLVTTGLGTTPGMTKRGLIWVVHKLQVVVDQYPSW 164

[Close]

BLAST of Lsi02G027620 vs. NCBI nr
Match: KAA0058801.1 (palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa] >TYK10596.1 palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa])

HSP 1 Score: 240.7 bits (613), Expect = 7.5e-60
Identity = 122/164 (74.39%), Postives = 132/164 (80.49%), Query Frame = 0


Query: 1   MLAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIAS 60
           M A+SSSY CN GKK NS LKKEQRFGD++ GNLQ K HPK+ LE L+TSNIIAKS+IAS
Sbjct: 1   MAAVSSSYCCNLGKKFNSNLKKEQRFGDSNTGNLQLKNHPKLFLEGLRTSNIIAKSSIAS 60

Query: 61  EVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFL----------------------E 120
           EVRKI+SYESR+LDITHGNLTMGEL+NDGLVYQRHFL                      E
Sbjct: 61  EVRKINSYESRNLDITHGNLTMGELLNDGLVYQRHFLVKSYESGPDKKMTMFFIINQLHE 120

Query: 121 SKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           SKLNHLKSCGLVTTGLGTTPEMTKRGL+WVVHKLQVVVDQYPSW
Sbjct: 121 SKLNHLKSCGLVTTGLGTTPEMTKRGLIWVVHKLQVVVDQYPSW 164

[Close]

BLAST of Lsi02G027620 vs. NCBI nr
Match: KAG6593401.1 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 129.0 bits (323), Expect = 3.2e-26
Identity = 79/169 (46.75%), Postives = 93/169 (55.03%), Query Frame = 0


Query: 1   MLAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIAS 60
           M  I SS   +FG ++ SI  +E  FG  +  +L  KG PK     LK+S+I     IA 
Sbjct: 10  MALICSSNGSHFGMELVSISTREPTFGHATTPSLHLKGRPKSASLTLKSSHI--SQPIAG 69

Query: 61  EVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFL----------------------E 120
             + ID Y SR  +IT  NL + E +NDGLVYQ HFL                      E
Sbjct: 70  GFKSIDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQE 129

Query: 121 SKLNHLKSCGLV-----TTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           SKLNH  SCGLV      TG GTT EMTKR L+WVVHKLQVVVDQ+PSW
Sbjct: 130 SKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSW 176

[Close]

BLAST of Lsi02G027620 vs. NCBI nr
Match: XP_023514527.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 127.1 bits (318), Expect = 1.2e-25
Identity = 78/169 (46.15%), Postives = 92/169 (54.44%), Query Frame = 0


Query: 1   MLAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIAS 60
           M  I SS   +FG ++ SI  +E  FG  +  +L  K  PK     LK+S+I     IA 
Sbjct: 1   MALICSSNGSHFGMELVSISMREPTFGHATTPSLHLKARPKTASLTLKSSHI--SQPIAG 60

Query: 61  EVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFL----------------------E 120
             + ID Y SR  +IT  NL + E +NDGLVYQ HFL                      E
Sbjct: 61  GFKSIDDYVSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQE 120

Query: 121 SKLNHLKSCGLV-----TTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           SKLNH  SCGLV      TG GTT EMTKR L+WVVHKLQVVVDQ+PSW
Sbjct: 121 SKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSW 167

[Close]

BLAST of Lsi02G027620 vs. NCBI nr
Match: XP_023004418.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 123.2 bits (308), Expect = 1.7e-24
Identity = 74/152 (48.68%), Postives = 86/152 (56.58%), Query Frame = 0


Query: 18  SILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIASEVRKIDSYESRSLDITH 77
           SI  +E  FG  +  +L  KG PK     LK+S+I     IAS  + +D Y SR  +IT 
Sbjct: 5   SISMREPTFGHATTPSLHLKGRPKSASLTLKSSHI--SQPIASGFKSMDDYVSRKPEITR 64

Query: 78  GNLTMGELVNDGLVYQRHFL----------------------ESKLNHLKSCGLVTTGL- 137
            NL + E +NDGLVYQ HFL                      ESKLNH  SCGLV TGL 
Sbjct: 65  ENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVKTGLG 124

Query: 138 ----GTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
               GTT EMTKR L+WVVHKLQVVVDQ+PSW
Sbjct: 125 KTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSW 154

[Close]

BLAST of Lsi02G027620 vs. TAIR 10
Match: AT1G08510.1 (fatty acyl-ACP thioesterases B )

HSP 1 Score: 71.2 bits (173), Expect = 7.3e-13
Identity = 35/97 (36.08%), Postives = 54/97 (55.67%), Query Frame = 0


Query: 68  YESRSLDITHGNLTMGELVNDGLVYQR----------------------HFLESKLNHLK 127
           ++ R  D+      +G +V DGLV+++                      H  E+ LNH+K
Sbjct: 115 WKPRRSDMLVDPFGIGRIVQDGLVFRQNFSIRSYEIGADRSASIETVMNHLQETALNHVK 174

Query: 128 SCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           + GL+  G G+TPEM K+ L+WVV ++QVVVD+YP+W
Sbjct: 175 TAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTW 211

[Close]

BLAST of Lsi02G027620 vs. TAIR 10
Match: AT3G25110.1 (fatA acyl-ACP thioesterase )

HSP 1 Score: 47.4 bits (111), Expect = 1.1e-05
Identity = 29/122 (23.77%), Postives = 50/122 (40.98%), Query Frame = 0


Query: 43  CLERLKTSNIIAKSTIASEVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFL----- 102
           C +  KT  +  ++ ++++   +     + L      L +G L  DGL Y+  F+     
Sbjct: 39  CSQTKKTGLVPLRAVVSADQGSV----VQGLATLADQLRLGSLTEDGLSYKEKFVVRSYE 98

Query: 103 -----------------ESKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYP 143
                            E   NH +S G  T G  TT  M K  L+WV  ++ + + +YP
Sbjct: 99  VGSNKTATVETIANLLQEVGCNHAQSVGFSTDGFATTTTMRKLHLIWVTARMHIEIYKYP 156

[Close]

BLAST of Lsi02G027620 vs. TAIR 10
Match: AT4G13050.1 (Acyl-ACP thioesterase )

HSP 1 Score: 44.7 bits (104), Expect = 7.4e-05
Identity = 28/105 (26.67%), Postives = 42/105 (40.00%), Query Frame = 0


Query: 60  SEVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFL---------------------- 119
           S VR  D+    S       L  G L+ DG  Y+  F+                      
Sbjct: 54  SSVRVADTVSGTS---PADRLRFGRLMEDGFSYKEKFIVRSYEVGINKTATIETIANLLQ 113

Query: 120 ESKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 143
           E   NH+++ G  T G  TT  M K  L+WV  ++ + + +YP+W
Sbjct: 114 EVACNHVQNVGFSTDGFATTLTMRKLHLIWVTARMHIEIYKYPAW 155

The following BLAST results are available for this feature:

BLAST of Lsi02G027620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SJE2	1.0e-11	36.08	Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thalia...	[more]
G3ESV0	1.8e-11	37.23	Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea ...	[more]
Q9SQI3	3.0e-11	35.05	Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum...	[more]
Q39513	1.9e-10	39.76	Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana ...	[more]
G3ESV1	7.4e-10	57.45	Acyl-[acyl-carrier-protein] hydrolase FATB3, chloroplastic OS=Cuphea viscosissim...	[more]

BLAST of Lsi02G027620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K767	5.6e-61	75.00	Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis sativus OX=3659 GN=Csa_7G440570...	[more]
A0A5A7UZ85	3.6e-60	74.39	Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A6J1KZF4	8.5e-25	48.68	Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC11149773...	[more]
A0A6J1H8Q2	4.6e-23	46.71	Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461...	[more]
A0A6J1CMR0	5.3e-11	75.56	Acyl-[acyl-carrier-protein] hydrolase OS=Momordica charantia OX=3673 GN=LOC11101...	[more]

BLAST of Lsi02G027620 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN45333.1	1.2e-60	75.00	hypothetical protein Csa_015751 [Cucumis sativus]	[more]
KAA0058801.1	7.5e-60	74.39	palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa] >TYK10596...	[more]
KAG6593401.1	3.2e-26	46.75	Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita a...	[more]
XP_023514527.1	1.2e-25	46.15	palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita pepo ...	[more]
XP_023004418.1	1.7e-24	48.68	palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita maxim...	[more]

BLAST of Lsi02G027620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08510.1	7.3e-13	36.08	fatty acyl-ACP thioesterases B	[more]
AT3G25110.1	1.1e-05	23.77	fatA acyl-ACP thioesterase	[more]
AT4G13050.1	7.4e-05	26.67	Acyl-ACP thioesterase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.129.10	Hotdog Thioesterase	coord: 89..142 e-value: 6.8E-7 score: 30.8
None	No IPR available	PANTHER	PTHR31727:SF2	PALMITOYL-ACYL CARRIER PROTEIN THIOESTERASE, CHLOROPLASTIC	coord: 95..142
IPR002864	Acyl-ACP thioesterase	PFAM	PF01643	Acyl-ACP_TE	coord: 94..142 e-value: 1.0E-4 score: 21.7
IPR045023	Acyl-[acyl-carrier-protein] hydrolase FATA/B	PANTHER	PTHR31727	OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTIC	coord: 95..142

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G027620.1	Lsi02G027620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0016297	acyl-[acyl-carrier-protein] hydrolase activity
molecular_function	GO:0016790	thiolester hydrolase activity