Lsi02G027600 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G027600
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionD-glycerate 3-kinase, chloroplastic isoform X1
Locationchr02: 33865585 .. 33870323 (+)
RNA-Seq ExpressionLsi02G027600
SyntenyLsi02G027600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGGAGAAAAAACGTTTCTAACAGATTTTTCTCAAGAGAACGAAAGAGAATTGTCATCTCAAATTCTCAATTCACATCCGCACAGCACAGCTTACGTCTCTCTCTCTCCTCTTTTTTTTTCCCTCTCTCTCTCTTCCCCCACCTTCCCACTCTGGCTCGTAAAAGTCGACCAAAAACCATAACCACGAAGACTTGGAAATGATAATTGATAACGAAACTCTCACAGTGAGCTTTAGATAAGAATGGCAACTCAGCCGCTATCTCCGACTTCAACTTTTCCCTCCCTTTTCTGTAGCAACAGTAATGGGGTCGGTTTTTATCAATTCCGTTCCTCCCCTTATTCCCATCATGCCTCTGTTTCTTCTTCTTCATTTCCGATGGCTAAAACCAACCAATCCATTTCTAGACTCTGTTATTTGCAGACCCATTTGCCCAAATCAGGTATTTTCATCTTACCCACTTGGTTTTCGTGGATTACTATCGGTGTTTATCAGGTTTTGTATATTTGGTTCTGGGATATCCTCTGTTTCGCATATTGGAATGGTTTTTGATGATTGTGAAGGGTAATTGGTTGTTGAGTGTTAGGAAGATTATGAGATGTTCTTCAATTTCAACTGCCTTTTTATTACCAAATGATGCTACTTTCTAAGTATGGGTTAACTTCAATGGAAATTATATGCGTTTGGTTCTGCGGTATTCTCTGTTTGAACATCTTGGGGAATAGTGTGGGGCCTTTCTTGATGTCTGAGCTAATATGGAGGGAAATTGTTGGGACTTCTTCTTCAGTTATGGAAATTTGGTTTCGTGATGCAAGAATGAAACGTTGGTTTTGTTTATTTAATTATTCAATTTCATTTGTTTTTAGAAATGGGTTCTGTTCATTCTAGTTGTTTGGAAGTTTTTGGTTTGTAACATATAGAGTAATCCCTTTTTTCTTTGTTCTCACATTTCTCTGTGGTATATTATCTTTTTTTGGTCTAACAAATGTGTGGTAGTTGAAGTTATATGTGGTTTATCACTTGTATCGTTCTACTTCGAGGTGGCAACAACAAAGTTAAGCCAGCTTGGGTCAAGCCTACAAAGAGCTAGTGGCCAAATAGTTTGGGCTACTGTTAAATGGGATGTGGGCTTTACTGTCTCTTATCAACTTTTGGCCTACATAGTTATTATCTATATATATATATATATATTCCCTTTTTCTCAGAGTTCTACAAGATTTTTAAACTTCTGACTTTTTGGGACGTAGATAATGTCTTGATCAATTGAGCTATGCAAATATTTTGTAGTTCTCATCATAGTTTTGTATTCTCATCTTGCCTTTTTCAAGACGAGGTACATAGCAGAAGATGTGATGGAAGGGGTGTTGAAAAGGTTGGTTTGGGCTCTCAAGGGCTAATGGTCAAGCGATGTTCAAGTCTTTCTTAACATGGGTTGAGAATGTGCTACCTTGATCATTAAGCCTTGAGGGGAAGACTTGACATATAATTATTAGTAGTTTCTTGGAAAAGTTTTACTCTCAGTCTACAACTTTGTCCTTTTCCCTTGTACTTCTTGGTTGTCTGCCATCATTTCAGTGTACTTATGGGAACAAAAAGGTTGCATTGGTATGGATAGGTCTTCATAATGGCCAAGGCCATTGGCATAACCCACTGCAAATGTGCAGATTGAGTAGATCATTCTGCTTGCCAATTAAACAATAGGGGTGGGTTAATCCAATAACACTGTTATATTCTTTTGACATTTCAACCTGGGGAAGAGGTCAGAGTGAAGTCATCAATTCAATCATTCACGTGGTCTCTTGACCTTTTATCTAAATGAACTAAACTTTTATACATTTGTCATAGAGATATGACCATTTCTCTAGATTGCAATTTGCACTTTCGTTTTAGATCGTTTTATTCTTACTTGAGACAATGCTTCACTACTTTCAAGGGACATTCTTATATGAGACTTATGCTAAGTAAAAGCGTTATATAAAGTTTGTACTTTGTAGTCAAATCAAAGAGAGCTTCTGAATTCTTGTATTTTATGAATGAACTTTTCAATATTTGTAGTGAAGCTTCTATATGTAGTTTCAGAAAGCTAAAGTTTTATTGTTTGATGGCCAACGCTGTTCTACCATATCTTGTATAGTGCACAATTGCGTTCTTAATGTGTAGAACGCTAACTACTTTAATTGTTGATCTCTTTCTTCTGTCTTCTTTGGGATTTTACTCTAGGCAATGGATGTTCATGGATTCAAGATAACTCCTTGTGCCACAGTGTTTCTTCCGTCGAGTATTATCAAGGCTCCCTTTGTTATGCATTACCCACAAAGCCAGCACAAGTTTCCACTTTGGAGGATCTTTTTGATTTCATTTGCTCAGGTCCCTTGATGGAAAAGTTGGGTTTGAGCCCCGAAAATGTGGCTGATGCCATAGATAAATGGCTAGCTTATGGCTCGTATCTTTGTCGATTGTTCAAAGCTAACGAAATGGAGCTCACAATTCCTCAGAAAGCAAGATTTTATCATTACTATATACCCGTCTTTCTTTGGTGTGAAGATCAGATTTCTCAACACCATTCCTCATTTAAAGAAGGAGAAGAAATTCCTCCCTTAGTGGTAAGCTTTTGGAATTCCCATATCTACTTTATTCTTGTTTCAAACCTGCTTATAAATGTTATTTGATACGTTCTTCCGCGTCAAGGAATTGAGATGTTCTGCATGAGGCTAGGCTTTCGTGGAAATTTTTTTATTTGAGGCCTATTGATTACGCTGGCTATGACTATAAGAGCTAAAAATTTGTTATTCCTCTGACTACTTGTCTTCAAATCACTTTGTTGTGCTTTATCAGATAGGATTTAGTGCTCCGCAAGGTTGTGGAAAGACGACACTCGTGTATGCTCTCAATTATCTTTTTGAAGTAACAGGCAGGTAGGCTGCGATGCTAGTAAATATGCCATAACGAGAGCTGACTATCTGCTGAAGCAGTGATAAGACTAAATAATGGGCAAAATATAAATTCTGACTGAAGTTCTATCTCAGGAAGTCTGCTATGTTATCCATTGACGACTTCTATCTGACAGCCGAGGGGCAGGTTGGTTCTATATATGGTGTTTTTAGCATTTGCTTTCCGTTTGCAGTACTATTAATAAACTAAGTGTTACATGGTAGGCACAACTGAGGGAAAGCAACCCCGGAAATGCACTTCTGGAGGTACTTATTTTCAGTCTCTCCTCCTCTTAAATTGTAGATAAATGAAAGCTGGTTGTAGTCCTCTATATTTCTTCTGTTAATTATTGTCTTCTATTCTTAGTTTCGTGGAAATGCTGGAAGTCATGATCTCGAATTGTCAGTCGAAACACTCACAGCGCTGTCCAAGTTGACCAAGGAAGGTCAGCTTCTCCTTCACTTTTGCTTTTGTTACCCCTATTTTGATTTTTTACTTATTTAGTTCTCTTTTAAGATGAAGGTTTAAAGATGAAAGTCCCCAGATATGATAAAGTAAGTGTAAATGGCCATTATCTTGTTTTGCTTATAGTTATATATAATTCCAGCCACTTATGATTCTCAAGCCCCCATGTCCTCTGTTTAATCCAGTCTGCATTTAGTGGAAGAGGTGACAGAGCTGATCCTTCTACTTGGCCAGAGGTTGAAGGACCACTGACAGTAAGAAATTTTAATTTAACAAAAAAGGAACTTAAATTTTTTTTGCACCTAGCTACATGAACTTTAATTTGGTTACCTCTAGGTCGTCCTATTTGAAGGCTGGATGCTTGGCTTCAAACCGCTCCCGACAGAAGCTGTCACAGCAGTCGATCCAGAGGTTATAGCTTTTCAACTTTCCAGAACTTATTTTCAAGATGATCCTTGTCAATCTATCATTAAGATCTACCATGTCCTTTTTATACTCAACCACTGTATCTTTTCAATCAAGTGAAAAAAGGAAAACAAAACATGTGAAAATCTCACTCAAATGAACTTCCAAATTTTACAGCTCGAGACAGTGAACAACAATATGAAAGCGTACTACGACGCTTGGGATAAGTACATTAAGGCGTGGATTGTGATCAAGATAAACGATCCCAGTTGCGTTTACAATTGGCGTCTACAGGTAAGCTGAGTTAACATTACCATACAGCCACCTGGGTTTGAGAAACCTTAAAAACAATCTGGTGTTATGAATTTGCAGGCAGAGATTGCAATGAGAGAGGCAGGAAAACCCGGGATGAGCGATGAAGAGGTAATGAAAACGAAAGGAAATAGAGTTTCAAAGTTGCTTATAGTAAGATGTTAGTGAAAAAGTTGTTTGTATGGATGAGAAGTTGTGGTAATGTTTGCAGGTTAGAGATTTTGTTTCACGTTATCTGCCAGCCTATAAGGCTTATCTTCCCACGTTGTATTCAGAAGGGCCAAGTGGATCAGATCCAAAGCGTCTGCTTGTGGTTGAAATTGATGAAGAAAGGAATCCAATTCTTGGTTATTAGGTGGTGATATATATGAAGTTAAAGTTGGTTTTTTCAGCTTTCACATGTTCTTTGACCACCTTTTTCTACCTCCTTATGTGGATACCACTAGCCTACTTTAAACAAAAGTAGGAGCTCGCTCTTATTCATTCATTTGTATCAATCTGTTTATGTTGTATGTAATAAATCAAGTCAAAAGTGGGATTTCACTCAAACTACATCAGCGACTAAGTAGTAATTAGGAGCTTTTACTTGTGGATTATACATTACTTTTTCTTTTTTCGATTAAAA

mRNA sequence

AAAAGGAGAAAAAACGTTTCTAACAGATTTTTCTCAAGAGAACGAAAGAGAATTGTCATCTCAAATTCTCAATTCACATCCGCACAGCACAGCTTACGTCTCTCTCTCTCCTCTTTTTTTTTCCCTCTCTCTCTCTTCCCCCACCTTCCCACTCTGGCTCGTAAAAGTCGACCAAAAACCATAACCACGAAGACTTGGAAATGATAATTGATAACGAAACTCTCACAGTGAGCTTTAGATAAGAATGGCAACTCAGCCGCTATCTCCGACTTCAACTTTTCCCTCCCTTTTCTGTAGCAACAGTAATGGGGTCGGTTTTTATCAATTCCGTTCCTCCCCTTATTCCCATCATGCCTCTGTTTCTTCTTCTTCATTTCCGATGGCTAAAACCAACCAATCCATTTCTAGACTCTGTTATTTGCAGACCCATTTGCCCAAATCAGGCAATGGATGTTCATGGATTCAAGATAACTCCTTGTGCCACAGTGTTTCTTCCGTCGAGTATTATCAAGGCTCCCTTTGTTATGCATTACCCACAAAGCCAGCACAAGTTTCCACTTTGGAGGATCTTTTTGATTTCATTTGCTCAGGTCCCTTGATGGAAAAGTTGGGTTTGAGCCCCGAAAATGTGGCTGATGCCATAGATAAATGGCTAGCTTATGGCTCGTATCTTTGTCGATTGTTCAAAGCTAACGAAATGGAGCTCACAATTCCTCAGAAAGCAAGATTTTATCATTACTATATACCCGTCTTTCTTTGGTGTGAAGATCAGATTTCTCAACACCATTCCTCATTTAAAGAAGGAGAAGAAATTCCTCCCTTAGTGATAGGATTTAGTGCTCCGCAAGGTTGTGGAAAGACGACACTCGTGTATGCTCTCAATTATCTTTTTGAAGTAACAGGCAGGAAGTCTGCTATGTTATCCATTGACGACTTCTATCTGACAGCCGAGGGGCAGGCACAACTGAGGGAAAGCAACCCCGGAAATGCACTTCTGGAGTTTCGTGGAAATGCTGGAAGTCATGATCTCGAATTGTCAGTCGAAACACTCACAGCGCTGTCCAAGTTGACCAAGGAAGATGAAGGTTTAAAGATGAAAGTCCCCAGATATGATAAATCTGCATTTAGTGGAAGAGGTGACAGAGCTGATCCTTCTACTTGGCCAGAGGTTGAAGGACCACTGACAGTCGTCCTATTTGAAGGCTGGATGCTTGGCTTCAAACCGCTCCCGACAGAAGCTGTCACAGCAGTCGATCCAGAGCTCGAGACAGTGAACAACAATATGAAAGCGTACTACGACGCTTGGGATAAGTACATTAAGGCGTGGATTGTGATCAAGATAAACGATCCCAGTTGCGTTTACAATTGGCGTCTACAGGCAGAGATTGCAATGAGAGAGGCAGGAAAACCCGGGATGAGCGATGAAGAGGTTAGAGATTTTGTTTCACGTTATCTGCCAGCCTATAAGGCTTATCTTCCCACGTTGTATTCAGAAGGGCCAAGTGGATCAGATCCAAAGCGTCTGCTTGTGGTTGAAATTGATGAAGAAAGGAATCCAATTCTTGGTTATTAGGTGGTGATATATATGAAGTTAAAGTTGGTTTTTTCAGCTTTCACATGTTCTTTGACCACCTTTTTCTACCTCCTTATGTGGATACCACTAGCCTACTTTAAACAAAAGTAGGAGCTCGCTCTTATTCATTCATTTGTATCAATCTGTTTATGTTGTATGTAATAAATCAAGTCAAAAGTGGGATTTCACTCAAACTACATCAGCGACTAAGTAGTAATTAGGAGCTTTTACTTGTGGATTATACATTACTTTTTCTTTTTTCGATTAAAA

Coding sequence (CDS)

ATGGCAACTCAGCCGCTATCTCCGACTTCAACTTTTCCCTCCCTTTTCTGTAGCAACAGTAATGGGGTCGGTTTTTATCAATTCCGTTCCTCCCCTTATTCCCATCATGCCTCTGTTTCTTCTTCTTCATTTCCGATGGCTAAAACCAACCAATCCATTTCTAGACTCTGTTATTTGCAGACCCATTTGCCCAAATCAGGCAATGGATGTTCATGGATTCAAGATAACTCCTTGTGCCACAGTGTTTCTTCCGTCGAGTATTATCAAGGCTCCCTTTGTTATGCATTACCCACAAAGCCAGCACAAGTTTCCACTTTGGAGGATCTTTTTGATTTCATTTGCTCAGGTCCCTTGATGGAAAAGTTGGGTTTGAGCCCCGAAAATGTGGCTGATGCCATAGATAAATGGCTAGCTTATGGCTCGTATCTTTGTCGATTGTTCAAAGCTAACGAAATGGAGCTCACAATTCCTCAGAAAGCAAGATTTTATCATTACTATATACCCGTCTTTCTTTGGTGTGAAGATCAGATTTCTCAACACCATTCCTCATTTAAAGAAGGAGAAGAAATTCCTCCCTTAGTGATAGGATTTAGTGCTCCGCAAGGTTGTGGAAAGACGACACTCGTGTATGCTCTCAATTATCTTTTTGAAGTAACAGGCAGGAAGTCTGCTATGTTATCCATTGACGACTTCTATCTGACAGCCGAGGGGCAGGCACAACTGAGGGAAAGCAACCCCGGAAATGCACTTCTGGAGTTTCGTGGAAATGCTGGAAGTCATGATCTCGAATTGTCAGTCGAAACACTCACAGCGCTGTCCAAGTTGACCAAGGAAGATGAAGGTTTAAAGATGAAAGTCCCCAGATATGATAAATCTGCATTTAGTGGAAGAGGTGACAGAGCTGATCCTTCTACTTGGCCAGAGGTTGAAGGACCACTGACAGTCGTCCTATTTGAAGGCTGGATGCTTGGCTTCAAACCGCTCCCGACAGAAGCTGTCACAGCAGTCGATCCAGAGCTCGAGACAGTGAACAACAATATGAAAGCGTACTACGACGCTTGGGATAAGTACATTAAGGCGTGGATTGTGATCAAGATAAACGATCCCAGTTGCGTTTACAATTGGCGTCTACAGGCAGAGATTGCAATGAGAGAGGCAGGAAAACCCGGGATGAGCGATGAAGAGGTTAGAGATTTTGTTTCACGTTATCTGCCAGCCTATAAGGCTTATCTTCCCACGTTGTATTCAGAAGGGCCAAGTGGATCAGATCCAAAGCGTCTGCTTGTGGTTGAAATTGATGAAGAAAGGAATCCAATTCTTGGTTATTAG

Protein sequence

MATQPLSPTSTFPSLFCSNSNGVGFYQFRSSPYSHHASVSSSSFPMAKTNQSISRLCYLQTHLPKSGNGCSWIQDNSLCHSVSSVEYYQGSLCYALPTKPAQVSTLEDLFDFICSGPLMEKLGLSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQHHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQAQLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGDRADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIKAWIVIKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGPSGSDPKRLLVVEIDEERNPILGY
Homology
BLAST of Lsi02G027600 vs. ExPASy Swiss-Prot
Match: Q944I4 (D-glycerate 3-kinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GLYK PE=1 SV=2)

HSP 1 Score: 604.4 bits (1557), Expect = 1.1e-171
Identity = 285/426 (66.90%), Postives = 345/426 (80.99%), Query Frame = 0

Query: 17  CSNSNGV-GFYQFRSSPYSHHASVSSSSFPMAKTNQSISRLCYLQTHLPKSGNGCSWIQD 76
           C +SN +   + F+   +S  +   +       ++   S+L  ++TH   +  GCSWIQD
Sbjct: 31  CHSSNPICNSFNFKRRSFSPSSPKFNDHVVNPSSSYLSSKLSPIRTHSSFAACGCSWIQD 90

Query: 77  NSLCHSVSSVEYYQGSLCYALP-TKPAQVSTLEDLFDFICSGPLMEKLGLSPENVADAID 136
           NS+ H  ++        C ALP T    VS++ DLF+FICSGPL+ K+G++P+ V  +ID
Sbjct: 91  NSMVHDYATTTNGTSKRCSALPTTNTVDVSSVSDLFEFICSGPLVNKIGITPQRVGQSID 150

Query: 137 KWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQHHSSFKEGEEIPPLV 196
           KWL YGS LCRLF+ NE++LTIPQKAR YHYYIPVF+WCEDQI+ H+S FK+G+++PPLV
Sbjct: 151 KWLLYGSQLCRLFQLNELKLTIPQKARLYHYYIPVFIWCEDQIALHNSKFKDGDDVPPLV 210

Query: 197 IGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQAQLRESNPGNALLEFR 256
           IGFSAPQGCGKTTLV+AL+YLF+ T +KSA +S+DDFYLTAEGQA+LR+ NPGNALLE+R
Sbjct: 211 IGFSAPQGCGKTTLVFALDYLFKTTKKKSATISVDDFYLTAEGQAELRKKNPGNALLEYR 270

Query: 257 GNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGDRADPSTWPEVEGPLT 316
           GNAGSHDL+LSVETL ALSKLTK  EGLKMKVPRY+KSA+SGRGDRAD STWPEVEGPL+
Sbjct: 271 GNAGSHDLKLSVETLEALSKLTK--EGLKMKVPRYNKSAYSGRGDRADSSTWPEVEGPLS 330

Query: 317 VVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIKAWIVIKINDPSCVYN 376
           V+LFEGWMLGFKPLP + V AVDP+LE VN N++AYYDAWDKYI AW+VIKI DPS VY 
Sbjct: 331 VILFEGWMLGFKPLPADVVKAVDPQLEVVNKNLEAYYDAWDKYIDAWVVIKIQDPSYVYR 390

Query: 377 WRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGPSGSDPKRLLVVEIDE 436
           WRLQAEIAMR+ G+ GMSDEEV DFVSRYLPAYKAYLPTLY+EGPSGSDP R+L ++IDE
Sbjct: 391 WRLQAEIAMRQDGQAGMSDEEVNDFVSRYLPAYKAYLPTLYAEGPSGSDPDRVLAIDIDE 450

Query: 437 ERNPIL 441
           ERNPIL
Sbjct: 451 ERNPIL 454

BLAST of Lsi02G027600 vs. ExPASy Swiss-Prot
Match: Q9UUH3 (Uncharacterized kinase mug58 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mug58 PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 8.1e-39
Identity = 95/259 (36.68%), Postives = 141/259 (54.44%), Query Frame = 0

Query: 192 PLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQAQLRESNPGNALL 251
           P ++G S PQG GK+TL  AL+             S+DDFYLT   Q +L ++NP N L+
Sbjct: 25  PFILGISGPQGSGKSTLASALDTELTRKNESVVKFSLDDFYLTHAEQVELAKNNPNNPLV 84

Query: 252 EFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGDRADPSTWPEVEG 311
           + RG AG+HD+      L A  K + E+    + +P YDKS F G GDR D S W +   
Sbjct: 85  QHRGLAGTHDVTFLNNVLNAFVKGSDEE----VSIPFYDKSKFGGYGDRGDESQWKKANP 144

Query: 312 PLTV-VLFEGWMLGFKPLPTEAVTA---------VDPELETVNNNMKAYYDAWDKYIKAW 371
             T  V+FEGWM+GF+PL +  ++          ++  L  VN  +  Y   + K I + 
Sbjct: 145 KTTTYVIFEGWMVGFEPLDSCMLSVRARSTRWQNIEGSLLWVNRKLADYQPIFQK-IDSL 204

Query: 372 IVIKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGPSG 431
           + ++  + + VY WRLQ E A++     GMSDEEV +FV+ Y+P Y  YL TL ++    
Sbjct: 205 VELEAQEINYVYRWRLQQEHALKARIHKGMSDEEVIEFVNHYMPQYVFYLGTLSNK--VH 264

Query: 432 SDPKRLLVVEIDEERNPIL 441
            +P  L ++ +DE R P++
Sbjct: 265 LNPHCLEII-LDENRYPVV 275

BLAST of Lsi02G027600 vs. ExPASy Swiss-Prot
Match: P42938 (Probable ATP-dependent kinase TDA10 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TDA10 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 3.9e-33
Identity = 95/254 (37.40%), Postives = 134/254 (52.76%), Query Frame = 0

Query: 192 PLVIGFSAPQGCGKT-TLVYALNYLFEVTGRKSAM--LSIDDFYLTAEGQAQLRESNPGN 251
           PL I FS PQG GK+ T +   N+L E  G + ++   SIDDFYLT E Q +L E    N
Sbjct: 31  PLFIFFSGPQGSGKSFTSIQIYNHLMEKYGGEKSIGYASIDDFYLTHEDQLKLNEQFKNN 90

Query: 252 ALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGDRADPSTWPE 311
            LL+ RG  G+HD++L  E L  +    +  +   + +P+YDKS F G GDR    T  +
Sbjct: 91  KLLQGRGLPGTHDMKLLQEVLNTIFNNNEHPDQDTVVLPKYDKSQFKGEGDRC--PTGQK 150

Query: 312 VEGPLTVVLFEGWMLGFKPL--PTEAVTAVDPELETVNNNMKAYYD-AW-DKYIKA-WIV 371
           ++ P+ + + EGW LGF P+    E    +  ++  VN  +  Y D  W +  IK+  IV
Sbjct: 151 IKLPVDIFILEGWFLGFNPILQGIENNDLLTGDMVDVNAKLFFYSDLLWRNPEIKSLGIV 210

Query: 372 IKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGPSGSD 431
              ++ + VY WRLQ E  +      GM+DE+V  FV RY+P+YK YL         GS 
Sbjct: 211 FTTDNINNVYGWRLQQEHELISKVGKGMTDEQVHAFVDRYMPSYKLYLNDFVRSESLGS- 270

Query: 432 PKRLLVVEIDEERN 438
               L + ID  RN
Sbjct: 271 -IATLTLGIDSNRN 280

BLAST of Lsi02G027600 vs. ExPASy TrEMBL
Match: A0A1S3CDU9 (D-glycerate 3-kinase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499753 PE=4 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.2e-247
Identity = 417/443 (94.13%), Postives = 429/443 (96.84%), Query Frame = 0

Query: 1   MATQPLSPTSTFPSLFCSNSNGVGFYQFRSSPYSHHASVSSSSFPMAKTNQSISRLCYLQ 60
           MATQPLS TSTFPSLFCSNSNGVGFY+FRSS YSHH SVSSSS PMAKTNQSISRLCYLQ
Sbjct: 1   MATQPLSSTSTFPSLFCSNSNGVGFYKFRSSLYSHHVSVSSSSLPMAKTNQSISRLCYLQ 60

Query: 61  THLPKSGNGCSWIQDNSLCHSVS-SVEYYQGSLCYALPTKPAQVSTLEDLFDFICSGPLM 120
           THLPKSGNGCSWIQDNSLCHSVS SVE Y+GS+CY+LPTKPAQVST+ DLF+FICSGPL+
Sbjct: 61  THLPKSGNGCSWIQDNSLCHSVSASVEGYRGSVCYSLPTKPAQVSTVADLFEFICSGPLI 120

Query: 121 EKLGLSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQ 180
           EKLGLSPE+VADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVF WCEDQISQ
Sbjct: 121 EKLGLSPESVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFFWCEDQISQ 180

Query: 181 HHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240
           H SSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA
Sbjct: 181 HQSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240

Query: 241 QLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGD 300
           QLRE NPGNALLE RGNAGSHDLELSVETLTALSKLTKED+GLKMK+PRYDKSAFSGRGD
Sbjct: 241 QLRERNPGNALLELRGNAGSHDLELSVETLTALSKLTKEDKGLKMKLPRYDKSAFSGRGD 300

Query: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIK 360
           RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDP+LETVNNNMK YYDAWDKYIK
Sbjct: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPQLETVNNNMKVYYDAWDKYIK 360

Query: 361 AWIVIKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGP 420
           AWIVIKINDPSCVY WRLQAEIAMREAGKPGM+DEEVRDFVSRYLPAYKAYLP LYSEGP
Sbjct: 361 AWIVIKINDPSCVYYWRLQAEIAMREAGKPGMTDEEVRDFVSRYLPAYKAYLPVLYSEGP 420

Query: 421 SGSDPKRLLVVEIDEERNPILGY 443
           SGSDPKRLLVVEIDEERNPILGY
Sbjct: 421 SGSDPKRLLVVEIDEERNPILGY 443

BLAST of Lsi02G027600 vs. ExPASy TrEMBL
Match: A0A1S3CDU8 (D-glycerate 3-kinase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499753 PE=4 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 2.0e-245
Identity = 416/443 (93.91%), Postives = 427/443 (96.39%), Query Frame = 0

Query: 1   MATQPLSPTSTFPSLFCSNSNGVGFYQFRSSPYSHHASVSSSSFPMAKTNQSISRLCYLQ 60
           MATQPLS TSTFPSLFCSNSNGVGFY+FRSS YSHH SVSSSS PMAKTNQSISRLCYLQ
Sbjct: 1   MATQPLSSTSTFPSLFCSNSNGVGFYKFRSSLYSHHVSVSSSSLPMAKTNQSISRLCYLQ 60

Query: 61  THLPKSGNGCSWIQDNSLCHSVS-SVEYYQGSLCYALPTKPAQVSTLEDLFDFICSGPLM 120
           THLPKSGNGCSWIQDNSLCHSVS SVE Y+GS+CY+LPTKPAQVST+ DLF+FICSGPL+
Sbjct: 61  THLPKSGNGCSWIQDNSLCHSVSASVEGYRGSVCYSLPTKPAQVSTVADLFEFICSGPLI 120

Query: 121 EKLGLSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQ 180
           EKLGLSPE+VADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVF WCEDQISQ
Sbjct: 121 EKLGLSPESVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFFWCEDQISQ 180

Query: 181 HHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240
           H SSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA
Sbjct: 181 HQSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240

Query: 241 QLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGD 300
           QLRE NPGNALLE RGNAGSHDLELSVETLTALSKLTK  EGLKMK+PRYDKSAFSGRGD
Sbjct: 241 QLRERNPGNALLELRGNAGSHDLELSVETLTALSKLTK--EGLKMKLPRYDKSAFSGRGD 300

Query: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIK 360
           RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDP+LETVNNNMK YYDAWDKYIK
Sbjct: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPQLETVNNNMKVYYDAWDKYIK 360

Query: 361 AWIVIKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGP 420
           AWIVIKINDPSCVY WRLQAEIAMREAGKPGM+DEEVRDFVSRYLPAYKAYLP LYSEGP
Sbjct: 361 AWIVIKINDPSCVYYWRLQAEIAMREAGKPGMTDEEVRDFVSRYLPAYKAYLPVLYSEGP 420

Query: 421 SGSDPKRLLVVEIDEERNPILGY 443
           SGSDPKRLLVVEIDEERNPILGY
Sbjct: 421 SGSDPKRLLVVEIDEERNPILGY 441

BLAST of Lsi02G027600 vs. ExPASy TrEMBL
Match: A0A0A0K6H4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G435550 PE=4 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 9.1e-243
Identity = 410/443 (92.55%), Postives = 425/443 (95.94%), Query Frame = 0

Query: 1   MATQPLSPTSTFPSLFCSNSNGVGFYQFRSSPYSHHASV-SSSSFPMAKTNQSISRLCYL 60
           MATQ LSPT TFPSLFCSNSNGVGFY+F SSPYSHH SV SSSSFPMAKTNQS SRLCYL
Sbjct: 1   MATQLLSPTITFPSLFCSNSNGVGFYKFHSSPYSHHVSVSSSSSFPMAKTNQSTSRLCYL 60

Query: 61  QTHLPKSGNGCSWIQDNSLCHSVSSVEYYQGSLCYALPTKPAQVSTLEDLFDFICSGPLM 120
           QTHLPKSGNGCSWIQDNSL H+VS+ E Y+GS+CY+LPTKPAQVST+EDLF+FICSGPL+
Sbjct: 61  QTHLPKSGNGCSWIQDNSLFHNVSASECYRGSICYSLPTKPAQVSTVEDLFEFICSGPLI 120

Query: 121 EKLGLSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQ 180
           EKLGLSPE+VADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVF WCEDQISQ
Sbjct: 121 EKLGLSPESVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFFWCEDQISQ 180

Query: 181 HHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240
           H SSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA
Sbjct: 181 HQSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240

Query: 241 QLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGD 300
           QLRE NPGNALLEFRGNAGSHDLELSVETLTA+SKLTK  EGLKMK+PRYDKSA+SGRGD
Sbjct: 241 QLRERNPGNALLEFRGNAGSHDLELSVETLTAVSKLTK--EGLKMKLPRYDKSAYSGRGD 300

Query: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIK 360
           RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDP+LE VNNNMK YYDAWDKYIK
Sbjct: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPQLEIVNNNMKVYYDAWDKYIK 360

Query: 361 AWIVIKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGP 420
           AWIVIKINDPSCVY WRLQAEIAMREAGKPGM+DEEVRDFVSRYLPAYKAYLP LYSEGP
Sbjct: 361 AWIVIKINDPSCVYYWRLQAEIAMREAGKPGMTDEEVRDFVSRYLPAYKAYLPVLYSEGP 420

Query: 421 SGSDPKRLLVVEIDEERNPILGY 443
           SGSDPKRLLVVEIDEERNPILGY
Sbjct: 421 SGSDPKRLLVVEIDEERNPILGY 441

BLAST of Lsi02G027600 vs. ExPASy TrEMBL
Match: A0A6J1HBB0 (D-glycerate 3-kinase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461248 PE=4 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.2e-240
Identity = 406/444 (91.44%), Postives = 422/444 (95.05%), Query Frame = 0

Query: 1   MATQPLSPTSTFPSLFCSNSNGVGFYQFRSSPYSHHASV-SSSSFPMAKTNQSISRLCYL 60
           MA QPLSPTSTFPSLFC+N+NGVGFY+F +  +SHH SV SSSSF MAK NQSISRLCY 
Sbjct: 1   MAAQPLSPTSTFPSLFCTNTNGVGFYKFGAFLHSHHVSVSSSSSFSMAKPNQSISRLCYS 60

Query: 61  QTHLPKSGNGCSWIQDNSLCHSV-SSVEYYQGSLCYALPTKPAQVSTLEDLFDFICSGPL 120
            TH  KSGNGCSWIQDNSLCHSV +SVE YQGS+C+ALPTKPAQVS LEDLF+FICSGPL
Sbjct: 61  PTHFAKSGNGCSWIQDNSLCHSVAASVERYQGSVCHALPTKPAQVSILEDLFEFICSGPL 120

Query: 121 MEKLGLSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQIS 180
           +EKLGL+P NVADAIDKWLAYGSYLCRLFK NEMELTIPQKARFYHYYIPVF WCEDQIS
Sbjct: 121 IEKLGLTPTNVADAIDKWLAYGSYLCRLFKVNEMELTIPQKARFYHYYIPVFFWCEDQIS 180

Query: 181 QHHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQ 240
           QHHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAM+SIDDFYLTAEGQ
Sbjct: 181 QHHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMVSIDDFYLTAEGQ 240

Query: 241 AQLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRG 300
           AQLRESNPGNALLE+RGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSA+SGRG
Sbjct: 241 AQLRESNPGNALLEYRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAYSGRG 300

Query: 301 DRADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYI 360
           DRADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDP+LE VNNNMKAYYDAWDKYI
Sbjct: 301 DRADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPQLEIVNNNMKAYYDAWDKYI 360

Query: 361 KAWIVIKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEG 420
            AWIVIKINDPSCVY WRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLY EG
Sbjct: 361 GAWIVIKINDPSCVYYWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYKEG 420

Query: 421 PSGSDPKRLLVVEIDEERNPILGY 443
           PSGSDPKRLLVVEIDE+RNPILGY
Sbjct: 421 PSGSDPKRLLVVEIDEQRNPILGY 444

BLAST of Lsi02G027600 vs. ExPASy TrEMBL
Match: A0A6J1H9C8 (D-glycerate 3-kinase, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461248 PE=4 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.5e-237
Identity = 404/444 (90.99%), Postives = 420/444 (94.59%), Query Frame = 0

Query: 1   MATQPLSPTSTFPSLFCSNSNGVGFYQFRSSPYSHHASV-SSSSFPMAKTNQSISRLCYL 60
           MA QPLSPTSTFPSLFC+N+NGVGFY+F +  +SHH SV SSSSF MAK NQSISRLCY 
Sbjct: 1   MAAQPLSPTSTFPSLFCTNTNGVGFYKFGAFLHSHHVSVSSSSSFSMAKPNQSISRLCYS 60

Query: 61  QTHLPKSGNGCSWIQDNSLCHSV-SSVEYYQGSLCYALPTKPAQVSTLEDLFDFICSGPL 120
            TH  KSGNGCSWIQDNSLCHSV +SVE YQGS+C+ALPTKPAQVS LEDLF+FICSGPL
Sbjct: 61  PTHFAKSGNGCSWIQDNSLCHSVAASVERYQGSVCHALPTKPAQVSILEDLFEFICSGPL 120

Query: 121 MEKLGLSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQIS 180
           +EKLGL+P NVADAIDKWLAYGSYLCRLFK NEMELTIPQKARFYHYYIPVF WCEDQIS
Sbjct: 121 IEKLGLTPTNVADAIDKWLAYGSYLCRLFKVNEMELTIPQKARFYHYYIPVFFWCEDQIS 180

Query: 181 QHHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQ 240
           QHHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAM+SIDDFYLTAEGQ
Sbjct: 181 QHHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMVSIDDFYLTAEGQ 240

Query: 241 AQLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRG 300
           AQLRESNPGNALLE+RGNAGSHDLELSVETLTALSKLTK  EGLKMKVPRYDKSA+SGRG
Sbjct: 241 AQLRESNPGNALLEYRGNAGSHDLELSVETLTALSKLTK--EGLKMKVPRYDKSAYSGRG 300

Query: 301 DRADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYI 360
           DRADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDP+LE VNNNMKAYYDAWDKYI
Sbjct: 301 DRADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPQLEIVNNNMKAYYDAWDKYI 360

Query: 361 KAWIVIKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEG 420
            AWIVIKINDPSCVY WRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLY EG
Sbjct: 361 GAWIVIKINDPSCVYYWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYKEG 420

Query: 421 PSGSDPKRLLVVEIDEERNPILGY 443
           PSGSDPKRLLVVEIDE+RNPILGY
Sbjct: 421 PSGSDPKRLLVVEIDEQRNPILGY 442

BLAST of Lsi02G027600 vs. NCBI nr
Match: XP_038899961.1 (D-glycerate 3-kinase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 885.2 bits (2286), Expect = 2.4e-253
Identity = 426/443 (96.16%), Postives = 435/443 (98.19%), Query Frame = 0

Query: 1   MATQPLSPTSTFPSLFCSNSNGVGFYQFRSSPYSHHASVSSSSFPMAKTNQSISRLCYLQ 60
           MATQPLSPTSTFPSLFCSNSNGVGFY+FRSS YSHHASVSSSSFPMAKTNQSISRLCYLQ
Sbjct: 1   MATQPLSPTSTFPSLFCSNSNGVGFYKFRSSSYSHHASVSSSSFPMAKTNQSISRLCYLQ 60

Query: 61  THLPKSGNGCSWIQDNSLCHSVS-SVEYYQGSLCYALPTKPAQVSTLEDLFDFICSGPLM 120
           THLPKSGNGCSWIQDNSLCHSVS S+E +QGS+CYALPTKPAQVST+EDLF+FICSGPLM
Sbjct: 61  THLPKSGNGCSWIQDNSLCHSVSASIECFQGSICYALPTKPAQVSTVEDLFEFICSGPLM 120

Query: 121 EKLGLSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQ 180
           EKLG+SPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVF WCEDQISQ
Sbjct: 121 EKLGMSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFFWCEDQISQ 180

Query: 181 HHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240
           H SSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA
Sbjct: 181 HQSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240

Query: 241 QLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGD 300
           QLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGD
Sbjct: 241 QLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGD 300

Query: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIK 360
           RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAV P+LETVNNNMKAYYDAWDKYIK
Sbjct: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVHPQLETVNNNMKAYYDAWDKYIK 360

Query: 361 AWIVIKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGP 420
           AWIVIKINDPSCVY WRLQAEIAMREAGKPGMSDEEV+DFVSRYLPAYKAYLPTLYSEGP
Sbjct: 361 AWIVIKINDPSCVYYWRLQAEIAMREAGKPGMSDEEVKDFVSRYLPAYKAYLPTLYSEGP 420

Query: 421 SGSDPKRLLVVEIDEERNPILGY 443
           SGSDPK LLVVEIDEERNPILGY
Sbjct: 421 SGSDPKHLLVVEIDEERNPILGY 443

BLAST of Lsi02G027600 vs. NCBI nr
Match: XP_038899962.1 (D-glycerate 3-kinase, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 876.3 bits (2263), Expect = 1.1e-250
Identity = 424/443 (95.71%), Postives = 433/443 (97.74%), Query Frame = 0

Query: 1   MATQPLSPTSTFPSLFCSNSNGVGFYQFRSSPYSHHASVSSSSFPMAKTNQSISRLCYLQ 60
           MATQPLSPTSTFPSLFCSNSNGVGFY+FRSS YSHHASVSSSSFPMAKTNQSISRLCYLQ
Sbjct: 1   MATQPLSPTSTFPSLFCSNSNGVGFYKFRSSSYSHHASVSSSSFPMAKTNQSISRLCYLQ 60

Query: 61  THLPKSGNGCSWIQDNSLCHSVS-SVEYYQGSLCYALPTKPAQVSTLEDLFDFICSGPLM 120
           THLPKSGNGCSWIQDNSLCHSVS S+E +QGS+CYALPTKPAQVST+EDLF+FICSGPLM
Sbjct: 61  THLPKSGNGCSWIQDNSLCHSVSASIECFQGSICYALPTKPAQVSTVEDLFEFICSGPLM 120

Query: 121 EKLGLSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQ 180
           EKLG+SPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVF WCEDQISQ
Sbjct: 121 EKLGMSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFFWCEDQISQ 180

Query: 181 HHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240
           H SSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA
Sbjct: 181 HQSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240

Query: 241 QLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGD 300
           QLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTK  EGLKMKVPRYDKSAFSGRGD
Sbjct: 241 QLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTK--EGLKMKVPRYDKSAFSGRGD 300

Query: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIK 360
           RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAV P+LETVNNNMKAYYDAWDKYIK
Sbjct: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVHPQLETVNNNMKAYYDAWDKYIK 360

Query: 361 AWIVIKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGP 420
           AWIVIKINDPSCVY WRLQAEIAMREAGKPGMSDEEV+DFVSRYLPAYKAYLPTLYSEGP
Sbjct: 361 AWIVIKINDPSCVYYWRLQAEIAMREAGKPGMSDEEVKDFVSRYLPAYKAYLPTLYSEGP 420

Query: 421 SGSDPKRLLVVEIDEERNPILGY 443
           SGSDPK LLVVEIDEERNPILGY
Sbjct: 421 SGSDPKHLLVVEIDEERNPILGY 441

BLAST of Lsi02G027600 vs. NCBI nr
Match: XP_008461051.1 (PREDICTED: D-glycerate 3-kinase, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 865.1 bits (2234), Expect = 2.5e-247
Identity = 417/443 (94.13%), Postives = 429/443 (96.84%), Query Frame = 0

Query: 1   MATQPLSPTSTFPSLFCSNSNGVGFYQFRSSPYSHHASVSSSSFPMAKTNQSISRLCYLQ 60
           MATQPLS TSTFPSLFCSNSNGVGFY+FRSS YSHH SVSSSS PMAKTNQSISRLCYLQ
Sbjct: 1   MATQPLSSTSTFPSLFCSNSNGVGFYKFRSSLYSHHVSVSSSSLPMAKTNQSISRLCYLQ 60

Query: 61  THLPKSGNGCSWIQDNSLCHSVS-SVEYYQGSLCYALPTKPAQVSTLEDLFDFICSGPLM 120
           THLPKSGNGCSWIQDNSLCHSVS SVE Y+GS+CY+LPTKPAQVST+ DLF+FICSGPL+
Sbjct: 61  THLPKSGNGCSWIQDNSLCHSVSASVEGYRGSVCYSLPTKPAQVSTVADLFEFICSGPLI 120

Query: 121 EKLGLSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQ 180
           EKLGLSPE+VADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVF WCEDQISQ
Sbjct: 121 EKLGLSPESVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFFWCEDQISQ 180

Query: 181 HHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240
           H SSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA
Sbjct: 181 HQSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240

Query: 241 QLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGD 300
           QLRE NPGNALLE RGNAGSHDLELSVETLTALSKLTKED+GLKMK+PRYDKSAFSGRGD
Sbjct: 241 QLRERNPGNALLELRGNAGSHDLELSVETLTALSKLTKEDKGLKMKLPRYDKSAFSGRGD 300

Query: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIK 360
           RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDP+LETVNNNMK YYDAWDKYIK
Sbjct: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPQLETVNNNMKVYYDAWDKYIK 360

Query: 361 AWIVIKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGP 420
           AWIVIKINDPSCVY WRLQAEIAMREAGKPGM+DEEVRDFVSRYLPAYKAYLP LYSEGP
Sbjct: 361 AWIVIKINDPSCVYYWRLQAEIAMREAGKPGMTDEEVRDFVSRYLPAYKAYLPVLYSEGP 420

Query: 421 SGSDPKRLLVVEIDEERNPILGY 443
           SGSDPKRLLVVEIDEERNPILGY
Sbjct: 421 SGSDPKRLLVVEIDEERNPILGY 443

BLAST of Lsi02G027600 vs. NCBI nr
Match: XP_008461052.1 (PREDICTED: D-glycerate 3-kinase, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 857.8 bits (2215), Expect = 4.0e-245
Identity = 416/443 (93.91%), Postives = 427/443 (96.39%), Query Frame = 0

Query: 1   MATQPLSPTSTFPSLFCSNSNGVGFYQFRSSPYSHHASVSSSSFPMAKTNQSISRLCYLQ 60
           MATQPLS TSTFPSLFCSNSNGVGFY+FRSS YSHH SVSSSS PMAKTNQSISRLCYLQ
Sbjct: 1   MATQPLSSTSTFPSLFCSNSNGVGFYKFRSSLYSHHVSVSSSSLPMAKTNQSISRLCYLQ 60

Query: 61  THLPKSGNGCSWIQDNSLCHSVS-SVEYYQGSLCYALPTKPAQVSTLEDLFDFICSGPLM 120
           THLPKSGNGCSWIQDNSLCHSVS SVE Y+GS+CY+LPTKPAQVST+ DLF+FICSGPL+
Sbjct: 61  THLPKSGNGCSWIQDNSLCHSVSASVEGYRGSVCYSLPTKPAQVSTVADLFEFICSGPLI 120

Query: 121 EKLGLSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQ 180
           EKLGLSPE+VADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVF WCEDQISQ
Sbjct: 121 EKLGLSPESVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFFWCEDQISQ 180

Query: 181 HHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240
           H SSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA
Sbjct: 181 HQSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240

Query: 241 QLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGD 300
           QLRE NPGNALLE RGNAGSHDLELSVETLTALSKLTK  EGLKMK+PRYDKSAFSGRGD
Sbjct: 241 QLRERNPGNALLELRGNAGSHDLELSVETLTALSKLTK--EGLKMKLPRYDKSAFSGRGD 300

Query: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIK 360
           RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDP+LETVNNNMK YYDAWDKYIK
Sbjct: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPQLETVNNNMKVYYDAWDKYIK 360

Query: 361 AWIVIKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGP 420
           AWIVIKINDPSCVY WRLQAEIAMREAGKPGM+DEEVRDFVSRYLPAYKAYLP LYSEGP
Sbjct: 361 AWIVIKINDPSCVYYWRLQAEIAMREAGKPGMTDEEVRDFVSRYLPAYKAYLPVLYSEGP 420

Query: 421 SGSDPKRLLVVEIDEERNPILGY 443
           SGSDPKRLLVVEIDEERNPILGY
Sbjct: 421 SGSDPKRLLVVEIDEERNPILGY 441

BLAST of Lsi02G027600 vs. NCBI nr
Match: XP_011659550.1 (D-glycerate 3-kinase, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 856.3 bits (2211), Expect = 1.2e-244
Identity = 411/443 (92.78%), Postives = 427/443 (96.39%), Query Frame = 0

Query: 1   MATQPLSPTSTFPSLFCSNSNGVGFYQFRSSPYSHHASV-SSSSFPMAKTNQSISRLCYL 60
           MATQ LSPT TFPSLFCSNSNGVGFY+F SSPYSHH SV SSSSFPMAKTNQS SRLCYL
Sbjct: 1   MATQLLSPTITFPSLFCSNSNGVGFYKFHSSPYSHHVSVSSSSSFPMAKTNQSTSRLCYL 60

Query: 61  QTHLPKSGNGCSWIQDNSLCHSVSSVEYYQGSLCYALPTKPAQVSTLEDLFDFICSGPLM 120
           QTHLPKSGNGCSWIQDNSL H+VS+ E Y+GS+CY+LPTKPAQVST+EDLF+FICSGPL+
Sbjct: 61  QTHLPKSGNGCSWIQDNSLFHNVSASECYRGSICYSLPTKPAQVSTVEDLFEFICSGPLI 120

Query: 121 EKLGLSPENVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQ 180
           EKLGLSPE+VADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVF WCEDQISQ
Sbjct: 121 EKLGLSPESVADAIDKWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFFWCEDQISQ 180

Query: 181 HHSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240
           H SSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA
Sbjct: 181 HQSSFKEGEEIPPLVIGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQA 240

Query: 241 QLRESNPGNALLEFRGNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGD 300
           QLRE NPGNALLEFRGNAGSHDLELSVETLTA+SKLTKED+GLKMK+PRYDKSA+SGRGD
Sbjct: 241 QLRERNPGNALLEFRGNAGSHDLELSVETLTAVSKLTKEDKGLKMKLPRYDKSAYSGRGD 300

Query: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIK 360
           RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDP+LE VNNNMK YYDAWDKYIK
Sbjct: 301 RADPSTWPEVEGPLTVVLFEGWMLGFKPLPTEAVTAVDPQLEIVNNNMKVYYDAWDKYIK 360

Query: 361 AWIVIKINDPSCVYNWRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGP 420
           AWIVIKINDPSCVY WRLQAEIAMREAGKPGM+DEEVRDFVSRYLPAYKAYLP LYSEGP
Sbjct: 361 AWIVIKINDPSCVYYWRLQAEIAMREAGKPGMTDEEVRDFVSRYLPAYKAYLPVLYSEGP 420

Query: 421 SGSDPKRLLVVEIDEERNPILGY 443
           SGSDPKRLLVVEIDEERNPILGY
Sbjct: 421 SGSDPKRLLVVEIDEERNPILGY 443

BLAST of Lsi02G027600 vs. TAIR 10
Match: AT1G80380.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 604.4 bits (1557), Expect = 7.5e-173
Identity = 285/426 (66.90%), Postives = 345/426 (80.99%), Query Frame = 0

Query: 17  CSNSNGV-GFYQFRSSPYSHHASVSSSSFPMAKTNQSISRLCYLQTHLPKSGNGCSWIQD 76
           C +SN +   + F+   +S  +   +       ++   S+L  ++TH   +  GCSWIQD
Sbjct: 31  CHSSNPICNSFNFKRRSFSPSSPKFNDHVVNPSSSYLSSKLSPIRTHSSFAACGCSWIQD 90

Query: 77  NSLCHSVSSVEYYQGSLCYALP-TKPAQVSTLEDLFDFICSGPLMEKLGLSPENVADAID 136
           NS+ H  ++        C ALP T    VS++ DLF+FICSGPL+ K+G++P+ V  +ID
Sbjct: 91  NSMVHDYATTTNGTSKRCSALPTTNTVDVSSVSDLFEFICSGPLVNKIGITPQRVGQSID 150

Query: 137 KWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQHHSSFKEGEEIPPLV 196
           KWL YGS LCRLF+ NE++LTIPQKAR YHYYIPVF+WCEDQI+ H+S FK+G+++PPLV
Sbjct: 151 KWLLYGSQLCRLFQLNELKLTIPQKARLYHYYIPVFIWCEDQIALHNSKFKDGDDVPPLV 210

Query: 197 IGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQAQLRESNPGNALLEFR 256
           IGFSAPQGCGKTTLV+AL+YLF+ T +KSA +S+DDFYLTAEGQA+LR+ NPGNALLE+R
Sbjct: 211 IGFSAPQGCGKTTLVFALDYLFKTTKKKSATISVDDFYLTAEGQAELRKKNPGNALLEYR 270

Query: 257 GNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGDRADPSTWPEVEGPLT 316
           GNAGSHDL+LSVETL ALSKLTK  EGLKMKVPRY+KSA+SGRGDRAD STWPEVEGPL+
Sbjct: 271 GNAGSHDLKLSVETLEALSKLTK--EGLKMKVPRYNKSAYSGRGDRADSSTWPEVEGPLS 330

Query: 317 VVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIKAWIVIKINDPSCVYN 376
           V+LFEGWMLGFKPLP + V AVDP+LE VN N++AYYDAWDKYI AW+VIKI DPS VY 
Sbjct: 331 VILFEGWMLGFKPLPADVVKAVDPQLEVVNKNLEAYYDAWDKYIDAWVVIKIQDPSYVYR 390

Query: 377 WRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGPSGSDPKRLLVVEIDE 436
           WRLQAEIAMR+ G+ GMSDEEV DFVSRYLPAYKAYLPTLY+EGPSGSDP R+L ++IDE
Sbjct: 391 WRLQAEIAMRQDGQAGMSDEEVNDFVSRYLPAYKAYLPTLYAEGPSGSDPDRVLAIDIDE 450

Query: 437 ERNPIL 441
           ERNPIL
Sbjct: 451 ERNPIL 454

BLAST of Lsi02G027600 vs. TAIR 10
Match: AT1G80380.4 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 586.6 bits (1511), Expect = 1.6e-167
Identity = 280/426 (65.73%), Postives = 339/426 (79.58%), Query Frame = 0

Query: 17  CSNSNGV-GFYQFRSSPYSHHASVSSSSFPMAKTNQSISRLCYLQTHLPKSGNGCSWIQD 76
           C +SN +   + F+   +S  +   +       ++   S+L  ++TH   +  GCSWIQD
Sbjct: 31  CHSSNPICNSFNFKRRSFSPSSPKFNDHVVNPSSSYLSSKLSPIRTHSSFAACGCSWIQD 90

Query: 77  NSLCHSVSSVEYYQGSLCYALP-TKPAQVSTLEDLFDFICSGPLMEKLGLSPENVADAID 136
           NS+ H  ++        C ALP T    VS++ DLF+FICSGPL+ K+G++P+ V  +ID
Sbjct: 91  NSMVHDYATTTNGTSKRCSALPTTNTVDVSSVSDLFEFICSGPLVNKIGITPQRVGQSID 150

Query: 137 KWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQHHSSFKEGEEIPPLV 196
           KWL YGS LCRLF+ NE++LTIPQKAR YHYYIPVF+WCEDQI+ H+S FK+G+++PPLV
Sbjct: 151 KWLLYGSQLCRLFQLNELKLTIPQKARLYHYYIPVFIWCEDQIALHNSKFKDGDDVPPLV 210

Query: 197 IGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQAQLRESNPGNALLEFR 256
           IGFSAPQGCGKTTLV+AL+YLF+ T +KSA +S+DDFYLTAEGQA+LR+ NPGNALLE+R
Sbjct: 211 IGFSAPQGCGKTTLVFALDYLFKTTKKKSATISVDDFYLTAEGQAELRKKNPGNALLEYR 270

Query: 257 GNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGDRADPSTWPEVEGPLT 316
           GNAGSHDL+LSVETL ALSKLTK  EGLKM      KSA+SGRGDRAD STWPEVEGPL+
Sbjct: 271 GNAGSHDLKLSVETLEALSKLTK--EGLKM------KSAYSGRGDRADSSTWPEVEGPLS 330

Query: 317 VVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIKAWIVIKINDPSCVYN 376
           V+LFEGWMLGFKPLP + V AVDP+LE VN N++AYYDAWDKYI AW+VIKI DPS VY 
Sbjct: 331 VILFEGWMLGFKPLPADVVKAVDPQLEVVNKNLEAYYDAWDKYIDAWVVIKIQDPSYVYR 390

Query: 377 WRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGPSGSDPKRLLVVEIDE 436
           WRLQAEIAMR+ G+ GMSDEEV DFVSRYLPAYKAYLPTLY+EGPSGSDP R+L ++IDE
Sbjct: 391 WRLQAEIAMRQDGQAGMSDEEVNDFVSRYLPAYKAYLPTLYAEGPSGSDPDRVLAIDIDE 448

Query: 437 ERNPIL 441
           ERNPIL
Sbjct: 451 ERNPIL 448

BLAST of Lsi02G027600 vs. TAIR 10
Match: AT1G80380.3 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 578.9 bits (1491), Expect = 3.4e-165
Identity = 267/364 (73.35%), Postives = 314/364 (86.26%), Query Frame = 0

Query: 78  LCHSVSSVEYYQGSLCYALP-TKPAQVSTLEDLFDFICSGPLMEKLGLSPENVADAIDKW 137
           + H  ++        C ALP T    VS++ DLF+FICSGPL+ K+G++P+ V  +IDKW
Sbjct: 1   MVHDYATTTNGTSKRCSALPTTNTVDVSSVSDLFEFICSGPLVNKIGITPQRVGQSIDKW 60

Query: 138 LAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQHHSSFKEGEEIPPLVIG 197
           L YGS LCRLF+ NE++LTIPQKAR YHYYIPVF+WCEDQI+ H+S FK+G+++PPLVIG
Sbjct: 61  LLYGSQLCRLFQLNELKLTIPQKARLYHYYIPVFIWCEDQIALHNSKFKDGDDVPPLVIG 120

Query: 198 FSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQAQLRESNPGNALLEFRGN 257
           FSAPQGCGKTTLV+AL+YLF+ T +KSA +S+DDFYLTAEGQA+LR+ NPGNALLE+RGN
Sbjct: 121 FSAPQGCGKTTLVFALDYLFKTTKKKSATISVDDFYLTAEGQAELRKKNPGNALLEYRGN 180

Query: 258 AGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGDRADPSTWPEVEGPLTVV 317
           AGSHDL+LSVETL ALSKLTK  EGLKMKVPRY+KSA+SGRGDRAD STWPEVEGPL+V+
Sbjct: 181 AGSHDLKLSVETLEALSKLTK--EGLKMKVPRYNKSAYSGRGDRADSSTWPEVEGPLSVI 240

Query: 318 LFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIKAWIVIKINDPSCVYNWR 377
           LFEGWMLGFKPLP + V AVDP+LE VN N++AYYDAWDKYI AW+VIKI DPS VY WR
Sbjct: 241 LFEGWMLGFKPLPADVVKAVDPQLEVVNKNLEAYYDAWDKYIDAWVVIKIQDPSYVYRWR 300

Query: 378 LQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGPSGSDPKRLLVVEIDEER 437
           LQAEIAMR+ G+ GMSDEEV DFVSRYLPAYKAYLPTLY+EGPSGSDP R+L ++IDEER
Sbjct: 301 LQAEIAMRQDGQAGMSDEEVNDFVSRYLPAYKAYLPTLYAEGPSGSDPDRVLAIDIDEER 360

Query: 438 NPIL 441
           NPIL
Sbjct: 361 NPIL 362

BLAST of Lsi02G027600 vs. TAIR 10
Match: AT1G80380.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 408.3 bits (1048), Expect = 7.9e-114
Identity = 207/426 (48.59%), Postives = 256/426 (60.09%), Query Frame = 0

Query: 17  CSNSNGV-GFYQFRSSPYSHHASVSSSSFPMAKTNQSISRLCYLQTHLPKSGNGCSWIQD 76
           C +SN +   + F+   +S  +   +       ++   S+L  ++TH   +  GCSWIQD
Sbjct: 31  CHSSNPICNSFNFKRRSFSPSSPKFNDHVVNPSSSYLSSKLSPIRTHSSFAACGCSWIQD 90

Query: 77  NSLCHSVSSVEYYQGSLCYALP-TKPAQVSTLEDLFDFICSGPLMEKLGLSPENVADAID 136
           NS+ H  ++        C ALP T    VS++ DLF+FICSGPL+ K+G++P+ V  +ID
Sbjct: 91  NSMVHDYATTTNGTSKRCSALPTTNTVDVSSVSDLFEFICSGPLVNKIGITPQRVGQSID 150

Query: 137 KWLAYGSYLCRLFKANEMELTIPQKARFYHYYIPVFLWCEDQISQHHSSFKEGEEIPPLV 196
           KWL YGS LCRLF+ NE++LTIPQKAR YHYYIPVF+WCEDQI+ H+S FK+G++     
Sbjct: 151 KWLLYGSQLCRLFQLNELKLTIPQKARLYHYYIPVFIWCEDQIALHNSKFKDGDD----- 210

Query: 197 IGFSAPQGCGKTTLVYALNYLFEVTGRKSAMLSIDDFYLTAEGQAQLRESNPGNALLEFR 256
                                                                       
Sbjct: 211 ------------------------------------------------------------ 270

Query: 257 GNAGSHDLELSVETLTALSKLTKEDEGLKMKVPRYDKSAFSGRGDRADPSTWPEVEGPLT 316
                                          VPRY+KSA+SGRGDRAD STWPEVEGPL+
Sbjct: 271 -------------------------------VPRYNKSAYSGRGDRADSSTWPEVEGPLS 330

Query: 317 VVLFEGWMLGFKPLPTEAVTAVDPELETVNNNMKAYYDAWDKYIKAWIVIKINDPSCVYN 376
           V+LFEGWMLGFKPLP + V AVDP+LE VN N++AYYDAWDKYI AW+VIKI DPS VY 
Sbjct: 331 VILFEGWMLGFKPLPADVVKAVDPQLEVVNKNLEAYYDAWDKYIDAWVVIKIQDPSYVYR 360

Query: 377 WRLQAEIAMREAGKPGMSDEEVRDFVSRYLPAYKAYLPTLYSEGPSGSDPKRLLVVEIDE 436
           WRLQAEIAMR+ G+ GMSDEEV DFVSRYLPAYKAYLPTLY+EGPSGSDP R+L ++IDE
Sbjct: 391 WRLQAEIAMRQDGQAGMSDEEVNDFVSRYLPAYKAYLPTLYAEGPSGSDPDRVLAIDIDE 360

Query: 437 ERNPIL 441
           ERNPIL
Sbjct: 451 ERNPIL 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q944I41.1e-17166.90D-glycerate 3-kinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GLYK PE=1... [more]
Q9UUH38.1e-3936.68Uncharacterized kinase mug58 OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
P429383.9e-3337.40Probable ATP-dependent kinase TDA10 OS=Saccharomyces cerevisiae (strain ATCC 204... [more]
Match NameE-valueIdentityDescription
A0A1S3CDU91.2e-24794.13D-glycerate 3-kinase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A1S3CDU82.0e-24593.91D-glycerate 3-kinase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0K6H49.1e-24392.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G435550 PE=4 SV=1[more]
A0A6J1HBB03.2e-24091.44D-glycerate 3-kinase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=366... [more]
A0A6J1H9C81.5e-23790.99D-glycerate 3-kinase, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=366... [more]
Match NameE-valueIdentityDescription
XP_038899961.12.4e-25396.16D-glycerate 3-kinase, chloroplastic isoform X1 [Benincasa hispida][more]
XP_038899962.11.1e-25095.71D-glycerate 3-kinase, chloroplastic isoform X2 [Benincasa hispida][more]
XP_008461051.12.5e-24794.13PREDICTED: D-glycerate 3-kinase, chloroplastic isoform X1 [Cucumis melo][more]
XP_008461052.14.0e-24593.91PREDICTED: D-glycerate 3-kinase, chloroplastic isoform X2 [Cucumis melo][more]
XP_011659550.11.2e-24492.78D-glycerate 3-kinase, chloroplastic isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G80380.27.5e-17366.90P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G80380.41.6e-16765.73P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G80380.33.4e-16573.35P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G80380.17.9e-11448.59P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 168..440
e-value: 4.8E-81
score: 273.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 192..420
NoneNo IPR availablePANTHERPTHR10285:SF178BNAC06G40610D PROTEINcoord: 77..440
NoneNo IPR availablePANTHERPTHR10285URIDINE KINASEcoord: 77..440

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G027600.1Lsi02G027600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0009853 photorespiration
cellular_component GO:0009507 chloroplast
molecular_function GO:0008887 glycerate kinase activity