Lsi02G027260 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G027260
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	DNA replication complex GINS protein PSF1-like
Location	chr02: 33592350 .. 33595563 (+)
RNA-Seq Expression	Lsi02G027260
Synteny	Lsi02G027260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTCATCACTTATTCCAAGTTTTCAGCTCGAGCTCAACTTTTCACCTTTCTCCTCCAAGCTCCCCGCCGTCGAACGTCTTCTTCGCCGCAGCGCCGTCGAACAGACGGACAACCATCGGATTCTTCCTCTTCTTTCTCTTTTCGGCTTGATTTTTCCTCAGATTCCCGATTCACCGGCCCCAATAAAGCAATAAAAGCTCAAAACTGAGAAGAACCGAACCGATCGGTCTGTTTCCTTAATTGAGATTCGGTTGGATTCTCGATTGGGCTACAAAACCGAACCGAGTATCGCCTTTCCCTGACCCACAACTTGATTTCCCGCCAGTTCGTCTATTAAATTGATATTTACATAAAGCAAAGCGCCCAAATCCCTAATTCAGAACTTCGTTCCTCCAGTTCTTCTACTCTTTTTCTCTGCTCAACGGAACAGGAATTCAATTACCAGCTGATGCTCAGAAGTTTTTCGATAGGTACTTTCAATTTGCAATTGAAACCCTATTTTTCTCTTTCTCCACCATCTATCTCTGGTTTAGATTTGATTTGCGTAATTTATTTCGAGCAGGTCTCGATCTCTGTATATCTTGCTTAGTAACAAGGATTTGGAATGAAATTTTTTCGTCAGGGAGGTGAAATCTGAATATGTATGGAAGAAAAGCATGCCAGTTGGTGAAAGAGTTAGCAAGCGGCGAGAAAGGACAGCTAACACACTTCAATGTAAGTTTACTTGTTGCATATATGTATCAACTGAGGTTGTGCCCGAATATTCTTGTTCTGAGTTGAAATTAATTATAGTTGATGAAATATTTGCACTACTTGACAAGTGTTTTATTTTAATAAACATAGTGCCTGTTTGGATTTTCTTGTAAGTGCTTAAAAAAGTGTTTTTAAGCATTTAGAAAATCAATCTAACTGCAACAAATATATTTGGCTTGTTTTCACTATTACCGGGTTCTTTTGAAGTGACTGTTATACACTTGGAGCTAATTGGCAGAAGCATTGGTCGACTCATAAATGTTCTTTACTTTTCTGTCTCTTCTTTCCCTCCATCTTTAGAAAATTTGTATTATATCTAATTTTTTTCCCTTTTGTTTTTCTACTTGTTTTTCCCTGTGTTTCTTCAGAGCGACCTATTTGAACAGGTTATTTCAGAATGCCAACAGCATCACCTTGAGCTTCAATCCTTGATTAGGTTAAAGATTCTACCTTCTTGTTCACACGTTTCATTAATTGATATGATATTTAGTTTTAGCCGGTAGTTGCAGGCTATCTTTAAATAGTTGTTACTGAACTGTTTGGTAGCAGAAAAAAGAAGGTAGTTAAACTGCTTCTAAGAGCGTGCTCACTTGGTTAATAACAGTTCAGCTGTATATAAGCAAGGAAAGCAGTCTTGAGAAATGGTAGAGGCTCACATTTTCTTCATAGTGGTTTTATCACGGATATTGCTTTTGGTCTCGTATGGCCTTATTGTGAATACCATCAACTGATATTTCTTTTATCCAAACTCTCTCCACTATGGCCTCCTTATATGGCATGAAGGCTTCAATAACAGAAGTCAACTCAAGGCTTCATTTATTTACAGTAGCACTTGGGAACTTTCCTTATCCTATGGACAGGTGTCCTTGGTTGACTTGAGGCTAACTATAAGATAGGAACTTGAGGTAATTTTAACCTTTTAATAAGTTCATCTATTTTCCAACGAAATCCATGTTCTATAGTTGCAATCTGCATAATAGGAACTATCCCCATAAGTTCACTAATCATTATAGTCCTGAACTTACTGCAGTATATTTTCGACTTATTGTGTCCCTCCAAGATTGAGCTGGTACATGTAGAAAGGAATGTACACATGGAGATGACTCAAGACATTTTCTATCTCATTAAAGTACTGAAGTGTGAAAGGAGGATTTTTCATCATAATCCTAAGCATTGTGCTTACATCTGGTTGAAGGTTCATCTAATTAACTTGGAAGGTTAATCCAAATTCTAAATCTTGTCATGTCCCTTAAACAAAAGACCAGATGGTGAAAGTTTACTAGCCATGTAGTCACTGTCGCTCTGGTTGTGAGAGCGCCCAAACCAAGGATTTCCAACTCAGTTATTCTCCATTCTAATTAGTCTTCAATTTTTGCGAGTAAAGAGCAAAAGTGCTTTAGTTATATGCTTTGAAGTTCTGGTTTAGATACATTGGCTATTATTATAAATTAAAAGGTACTATTTGCAGCCTCAATATATAGCTGATATGATAATCTGAAACAGGAAAGTACAGGAAGAAGGATTAGATCTTCAGACAACTAAGAATGAAGATCATTTTGGTGCGCTCATTCACCACCTTGCATTAGTTCGCAATAAACGTTGCCTTATGGCTTATGTGTAAGTGTAATATTCTGAAATAACTTTGAGGAACTTCCTTTTTCCTGGTTTACTGGGTTTGAGATAATTCTATTGTTATTTTCAAATCAAATGTCTTTATACATGAACATGCAGGCATAATCGAGCTGAGATTATAAGAAGTTTGATATGGAAGATAGTAGGATCTATGATTCCACCGGAAATACAAGAGAAGCTCAGCAACTCAGAGGAAGAGTATTTTAAGAAGCATTCTGCACGTCTGAAAGAATATATGTCAAAACTGGAGTTGGATTTGACTGTGGTAATTTCAAACACCAGGCTTTACAATAACACAATCTTTACATTTGGAACTCGAATTCATCGGCCTGAAATTTCCAAATTAATCATTGGCAGGATATGGTGCCACCTAAGGATCCTTATATCCAAGTGAGGGTGCTCGATGATATCGGTGAAGGAATCGTACTGAGTGATGATAAAACAGCAAATTTTGCACTTCATTCCATACATTTTCTTAAACGAACAGACGCTGAACAATACATCTCACGGGTGGGAATCTCTGTAAACTTTGGTAATTCTTGTTATTGCTTGCTTAAATGTCTCCTAAACTTGTAATCTTTCAAAACAGGGATTAATGGAGGAGCTTAGAGGCTAACTGTTCCACTGAAGATGAAGTTTAGTTGGAGCTGTTTGTTGTAAATTAAGAGCCAAACGAGACAAATGTGATCCATCCCATTTATTTGATTTTCATGTTTCAATTACATGTATAGATTTAGTTTTCATGTTTCAATATTTTTATACATTTAGTAGTATTTATGTTTCAATTACTTTTATACATTTAATTTTCATTATTAGTTCCTTGCTGGT

mRNA sequence

TTTTCATCACTTATTCCAAGTTTTCAGCTCGAGCTCAACTTTTCACCTTTCTCCTCCAAGCTCCCCGCCGTCGAACGTCTTCTTCGCCGCAGCGCCGTCGAACAGACGGACAACCATCGGATTCTTCCTCTTCTTTCTCTTTTCGGCTTGATTTTTCCTCAGATTCCCGATTCACCGGCCCCAATAAAGCAATAAAAGCTCAAAACTGAGAAGAACCGAACCGATCGGTCTGTTTCCTTAATTGAGATTCGGTTGGATTCTCGATTGGGCTACAAAACCGAACCGAGTATCGCCTTTCCCTGACCCACAACTTGATTTCCCGCCAGTTCGTCTATTAAATTGATATTTACATAAAGCAAAGCGCCCAAATCCCTAATTCAGAACTTCGTTCCTCCAGTTCTTCTACTCTTTTTCTCTGCTCAACGGAACAGGAATTCAATTACCAGCTGATGCTCAGAAGTTTTTCGATAGGTCTCGATCTCTGTATATCTTGCTTAGTAACAAGGATTTGGAATGAAATTTTTTCGTCAGGGAGGTGAAATCTGAATATGTATGGAAGAAAAGCATGCCAGTTGGTGAAAGAGTTAGCAAGCGGCGAGAAAGGACAGCTAACACACTTCAATAGCGACCTATTTGAACAGGTTATTTCAGAATGCCAACAGCATCACCTTGAGCTTCAATCCTTGATTAGGAAAGTACAGGAAGAAGGATTAGATCTTCAGACAACTAAGAATGAAGATCATTTTGGTGCGCTCATTCACCACCTTGCATTAGTTCGCAATAAACGTTGCCTTATGGCTTATGTGCATAATCGAGCTGAGATTATAAGAAGTTTGATATGGAAGATAGTAGGATCTATGATTCCACCGGAAATACAAGAGAAGCTCAGCAACTCAGAGGAAGAGTATTTTAAGAAGCATTCTGCACGTCTGAAAGAATATATGTCAAAACTGGAGTTGGATTTGACTGTGGATATGGTGCCACCTAAGGATCCTTATATCCAAGTGAGGGTGCTCGATGATATCGGTGAAGGAATCGTACTGAGTGATGATAAAACAGCAAATTTTGCACTTCATTCCATACATTTTCTTAAACGAACAGACGCTGAACAATACATCTCACGGGGATTAATGGAGGAGCTTAGAGGCTAACTGTTCCACTGAAGATGAAGTTTAGTTGGAGCTGTTTGTTGTAAATTAAGAGCCAAACGAGACAAATGTGATCCATCCCATTTATTTGATTTTCATGTTTCAATTACATGTATAGATTTAGTTTTCATGTTTCAATATTTTTATACATTTAGTAGTATTTATGTTTCAATTACTTTTATACATTTAATTTTCATTATTAGTTCCTTGCTGGT

Coding sequence (CDS)

ATGTATGGAAGAAAAGCATGCCAGTTGGTGAAAGAGTTAGCAAGCGGCGAGAAAGGACAGCTAACACACTTCAATAGCGACCTATTTGAACAGGTTATTTCAGAATGCCAACAGCATCACCTTGAGCTTCAATCCTTGATTAGGAAAGTACAGGAAGAAGGATTAGATCTTCAGACAACTAAGAATGAAGATCATTTTGGTGCGCTCATTCACCACCTTGCATTAGTTCGCAATAAACGTTGCCTTATGGCTTATGTGCATAATCGAGCTGAGATTATAAGAAGTTTGATATGGAAGATAGTAGGATCTATGATTCCACCGGAAATACAAGAGAAGCTCAGCAACTCAGAGGAAGAGTATTTTAAGAAGCATTCTGCACGTCTGAAAGAATATATGTCAAAACTGGAGTTGGATTTGACTGTGGATATGGTGCCACCTAAGGATCCTTATATCCAAGTGAGGGTGCTCGATGATATCGGTGAAGGAATCGTACTGAGTGATGATAAAACAGCAAATTTTGCACTTCATTCCATACATTTTCTTAAACGAACAGACGCTGAACAATACATCTCACGGGGATTAATGGAGGAGCTTAGAGGCTAA

Protein sequence

MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTTKNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEYFKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHFLKRTDAEQYISRGLMEELRG

Homology

[Close]

BLAST of Lsi02G027260 vs. ExPASy Swiss-Prot
Match: Q54HR6 (Probable DNA replication complex GINS protein PSF1 OS=Dictyostelium discoideum OX=44689 GN=gins1 PE=3 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.0e-24
Identity = 66/200 (33.00%), Postives = 115/200 (57.50%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60
           M+ + A +L+KEL   +   + H+N    +  I E    + +L   I + +E+       
Sbjct: 1   MFTKTAIELIKELRGTD--SIPHYNDTSIKATIDEMIALYEDLIKTITEHKEQ------- 60

Query: 61  KNEDHF--GALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEE 120
           K E  +   A+  H ++ R+KRC++AY++ R   I+   W    S++P +++E+LS +E 
Sbjct: 61  KKEPFYLQHAITFHNSINRDKRCILAYLNERLNRIKEYRWSSGQSLLPDQLKERLSQNEI 120

Query: 121 EYFKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSI 180
           ++F ++   L EY SK+ LDLT+D  PPK+ YI+VRV+ ++GE +VL+   T N  L++ 
Sbjct: 121 QFFSEYDKILTEYNSKVGLDLTIDPQPPKELYIEVRVIKELGE-VVLNSGCTVNLNLNTT 180

Query: 181 HFLKRTDAEQYISRGLMEEL 199
           HFLKR+D    +  G +E +
Sbjct: 181 HFLKRSDITNLVKNGSLEHI 190

[Close]

BLAST of Lsi02G027260 vs. ExPASy Swiss-Prot
Match: Q7ZT47 (DNA replication complex GINS protein PSF1 OS=Xenopus laevis OX=8355 GN=gins1 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.8e-23
Identity = 71/203 (34.98%), Postives = 110/203 (54.19%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEG-LDLQT 60
           M+  KA +L++EL     GQL  FN D   Q++ E +  + + Q+ + + + EG  DL  
Sbjct: 1   MFCEKAIELIRELQRASDGQLPAFNEDGIRQILEEMKALYEQNQADVNEAKTEGRSDLIP 60

Query: 61  TKNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEE 120
           T           H  L+RN+RC++AY+++R   IR+L W+  GS++P  ++  +S  E +
Sbjct: 61  T-------IKFRHCCLLRNRRCIVAYLYDRLLRIRALRWE-YGSVLPSALRFHMSTEEMD 120

Query: 121 YFKKHSARLKEYMSKL----ELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFAL 180
           +F ++   L  YM  L     LD+T DM PPK  YI+VR L D GE   + D  T     
Sbjct: 121 WFNQYKRSLATYMRSLGGEEGLDITQDMKPPKSLYIEVRCLRDYGE-FEIDDGTTILLKK 180

Query: 181 HSIHFLKRTDAEQYISRGLMEEL 199
           +S HFL R   EQ I +G++E +
Sbjct: 181 NSQHFLPRWKCEQLIRQGVLEHV 194

[Close]

BLAST of Lsi02G027260 vs. ExPASy Swiss-Prot
Match: Q9CZ15 (DNA replication complex GINS protein PSF1 OS=Mus musculus OX=10090 GN=Gins1 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.8e-22
Identity = 73/203 (35.96%), Postives = 109/203 (53.69%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGL-DLQT 60
           M+  KA +LV+EL    +GQL  FN D   QV+ E +  + + QS + + +  G  DL  
Sbjct: 1   MFCEKAMELVRELHRAPEGQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSAGRGDLIP 60

Query: 61  TKNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEE 120
           T           H AL+RN+RC +AY+++R   IR+L W+  GS++P  ++  +S  E E
Sbjct: 61  T-------VKFRHCALLRNRRCTIAYLYDRLLRIRALRWE-YGSVLPNSLRFHMSAEETE 120

Query: 121 YFKKHSARLKEYMSKL----ELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFAL 180
           +F  +   L  YM  L     LD+T D+ PPK  YI+VR L D GE   + D  +     
Sbjct: 121 WFNHYKKSLATYMRSLGGDEGLDITQDVKPPKSLYIEVRCLKDYGE-FEVDDGTSVLLKK 180

Query: 181 HSIHFLKRTDAEQYISRGLMEEL 199
           +S HFL R   EQ I +G++E +
Sbjct: 181 NSQHFLPRWKCEQLIRQGVLEHV 194

[Close]

BLAST of Lsi02G027260 vs. ExPASy Swiss-Prot
Match: A4IFH4 (DNA replication complex GINS protein PSF1 OS=Bos taurus OX=9913 GN=GINS1 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.3e-22
Identity = 71/203 (34.98%), Postives = 110/203 (54.19%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGL-DLQT 60
           M+  KA +LV+EL    +G+L  FN D   QV+ E +  + + QS + + +  G  DL  
Sbjct: 1   MFCEKAMELVRELHRAAEGRLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSSGRGDLIP 60

Query: 61  TKNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEE 120
           T           H +L+RN+RC +AY+++R   IR+L W+  GS++P  ++  +S  E +
Sbjct: 61  T-------IKFRHCSLLRNQRCTVAYLYDRLLRIRALRWE-YGSVLPSALRFHMSAEEMD 120

Query: 121 YFKKHSARLKEYMSKL----ELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFAL 180
           +F ++   L  YM  L     LD+T DM PPK  YI+VR L D GE   + D  +     
Sbjct: 121 WFNRYKKSLATYMRSLGGDEGLDITQDMKPPKSLYIEVRCLKDYGE-FEVEDGTSVLLKK 180

Query: 181 HSIHFLKRTDAEQYISRGLMEEL 199
           +S HFL R   EQ I +G++E +
Sbjct: 181 NSQHFLPRWKCEQLIRQGILEHV 194

[Close]

BLAST of Lsi02G027260 vs. ExPASy Swiss-Prot
Match: Q14691 (DNA replication complex GINS protein PSF1 OS=Homo sapiens OX=9606 GN=GINS1 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 7.0e-22
Identity = 71/203 (34.98%), Postives = 109/203 (53.69%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEG-LDLQT 60
           M+  KA +L++EL    +GQL  FN D   QV+ E +  + + QS + + +  G  DL  
Sbjct: 1   MFCEKAMELIRELHRAPEGQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSGGRSDLIP 60

Query: 61  TKNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEE 120
           T           H +L+RN+RC +AY+++R   IR+L W+  GS++P  ++  ++  E E
Sbjct: 61  T-------IKFRHCSLLRNRRCTVAYLYDRLLRIRALRWE-YGSVLPNALRFHMAAEEME 120

Query: 121 YFKKHSARLKEYMSKL----ELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFAL 180
           +F  +   L  YM  L     LD+T DM PPK  YI+VR L D GE   + D  +     
Sbjct: 121 WFNNYKRSLATYMRSLGGDEGLDITQDMKPPKSLYIEVRCLKDYGE-FEVDDGTSVLLKK 180

Query: 181 HSIHFLKRTDAEQYISRGLMEEL 199
           +S HFL R   EQ I +G++E +
Sbjct: 181 NSQHFLPRWKCEQLIRQGVLEHI 194

[Close]

BLAST of Lsi02G027260 vs. ExPASy TrEMBL
Match: A0A0A0K8U8 (Sld5 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G433190 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.2e-106
Identity = 197/200 (98.50%), Postives = 199/200 (99.50%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60
           MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT
Sbjct: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY 120
           KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSL+WK+VGSMIPPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLMWKVVGSMIPPEIQEKLSNSEEEY 120

Query: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180
           FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF
Sbjct: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQYISRGLMEELRG 201
           LKRTDAEQYISRGLMEEL G
Sbjct: 181 LKRTDAEQYISRGLMEELTG 200

[Close]

BLAST of Lsi02G027260 vs. ExPASy TrEMBL
Match: A0A1S3CDY2 (probable DNA replication complex GINS protein PSF1 OS=Cucumis melo OX=3656 GN=LOC103499784 PE=3 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 6.2e-106
Identity = 196/200 (98.00%), Postives = 198/200 (99.00%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60
           MYGRKACQLV ELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT
Sbjct: 1   MYGRKACQLVTELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY 120
           KNEDHFGALIHHLALVRNKRCLMAYVHNRAE IRSLIWK++GSMIPPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAETIRSLIWKLLGSMIPPEIQEKLSNSEEEY 120

Query: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180
           FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF
Sbjct: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQYISRGLMEELRG 201
           LKRTDAEQYISRGLMEELRG
Sbjct: 181 LKRTDAEQYISRGLMEELRG 200

[Close]

BLAST of Lsi02G027260 vs. ExPASy TrEMBL
Match: A0A5A7UU94 (Putative DNA replication complex GINS protein PSF1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002160 PE=3 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 6.2e-106
Identity = 196/200 (98.00%), Postives = 198/200 (99.00%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60
           MYGRKACQLV ELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT
Sbjct: 1   MYGRKACQLVTELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY 120
           KNEDHFGALIHHLALVRNKRCLMAYVHNRAE IRSLIWK++GSMIPPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAETIRSLIWKLLGSMIPPEIQEKLSNSEEEY 120

Query: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180
           FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF
Sbjct: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQYISRGLMEELRG 201
           LKRTDAEQYISRGLMEELRG
Sbjct: 181 LKRTDAEQYISRGLMEELRG 200

[Close]

BLAST of Lsi02G027260 vs. ExPASy TrEMBL
Match: A0A6J1KI79 (probable DNA replication complex GINS protein PSF1 OS=Cucurbita maxima OX=3661 GN=LOC111494653 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 9.8e-104
Identity = 190/200 (95.00%), Postives = 197/200 (98.50%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60
           MYGRKACQLVKELASGEKGQL  FNSDLFEQVISECQQHHLELQSL+RK+Q+EGLDLQTT
Sbjct: 1   MYGRKACQLVKELASGEKGQLAQFNSDLFEQVISECQQHHLELQSLLRKIQDEGLDLQTT 60

Query: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY 120
           KNEDHFGAL+HHLALVRNKRCLMAYV+NRAE IRSLIWKIVGSM+PPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALVHHLALVRNKRCLMAYVYNRAETIRSLIWKIVGSMLPPEIQEKLSNSEEEY 120

Query: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180
           FKKHSARLKEYMSK+ELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF
Sbjct: 121 FKKHSARLKEYMSKMELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQYISRGLMEELRG 201
           LKRTDAEQYISRGLMEELRG
Sbjct: 181 LKRTDAEQYISRGLMEELRG 200

[Close]

BLAST of Lsi02G027260 vs. ExPASy TrEMBL
Match: A0A6J1HHQ1 (probable DNA replication complex GINS protein PSF1 OS=Cucurbita moschata OX=3662 GN=LOC111464174 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 9.8e-104
Identity = 190/200 (95.00%), Postives = 197/200 (98.50%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60
           MYGRKACQLVKELASGEKGQL  FNSDLFEQVISECQQHHLELQSL+RK+Q+EGLDLQTT
Sbjct: 1   MYGRKACQLVKELASGEKGQLAQFNSDLFEQVISECQQHHLELQSLLRKIQDEGLDLQTT 60

Query: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY 120
           KNEDHFGAL+HHLALVRNKRCLMAYV+NRAE IRSLIWKIVGSM+PPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALVHHLALVRNKRCLMAYVYNRAETIRSLIWKIVGSMLPPEIQEKLSNSEEEY 120

Query: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180
           FKKHSARLKEYMSK+ELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF
Sbjct: 121 FKKHSARLKEYMSKMELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQYISRGLMEELRG 201
           LKRTDAEQYISRGLMEELRG
Sbjct: 181 LKRTDAEQYISRGLMEELRG 200

[Close]

BLAST of Lsi02G027260 vs. NCBI nr
Match: XP_038897311.1 (probable DNA replication complex GINS protein PSF1 [Benincasa hispida])

HSP 1 Score: 396.7 bits (1018), Expect = 1.2e-106
Identity = 198/200 (99.00%), Postives = 199/200 (99.50%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60
           MYGRKACQLVKEL SGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT
Sbjct: 1   MYGRKACQLVKELTSGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY 120
           KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY 120

Query: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180
           FKKHSAR+KEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF
Sbjct: 121 FKKHSARVKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQYISRGLMEELRG 201
           LKRTDAEQYISRGLMEELRG
Sbjct: 181 LKRTDAEQYISRGLMEELRG 200

[Close]

BLAST of Lsi02G027260 vs. NCBI nr
Match: XP_004135842.1 (probable DNA replication complex GINS protein PSF1 [Cucumis sativus] >KGN45289.1 hypothetical protein Csa_016815 [Cucumis sativus])

HSP 1 Score: 395.6 bits (1015), Expect = 2.6e-106
Identity = 197/200 (98.50%), Postives = 199/200 (99.50%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60
           MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT
Sbjct: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY 120
           KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSL+WK+VGSMIPPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLMWKVVGSMIPPEIQEKLSNSEEEY 120

Query: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180
           FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF
Sbjct: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQYISRGLMEELRG 201
           LKRTDAEQYISRGLMEEL G
Sbjct: 181 LKRTDAEQYISRGLMEELTG 200

[Close]

BLAST of Lsi02G027260 vs. NCBI nr
Match: XP_008461089.1 (PREDICTED: probable DNA replication complex GINS protein PSF1 [Cucumis melo] >XP_008461091.1 PREDICTED: probable DNA replication complex GINS protein PSF1 [Cucumis melo] >XP_008461092.1 PREDICTED: probable DNA replication complex GINS protein PSF1 [Cucumis melo] >XP_008461093.1 PREDICTED: probable DNA replication complex GINS protein PSF1 [Cucumis melo] >KAA0058768.1 putative DNA replication complex GINS protein PSF1 [Cucumis melo var. makuwa] >TYK10562.1 putative DNA replication complex GINS protein PSF1 [Cucumis melo var. makuwa])

HSP 1 Score: 393.3 bits (1009), Expect = 1.3e-105
Identity = 196/200 (98.00%), Postives = 198/200 (99.00%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60
           MYGRKACQLV ELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT
Sbjct: 1   MYGRKACQLVTELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY 120
           KNEDHFGALIHHLALVRNKRCLMAYVHNRAE IRSLIWK++GSMIPPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAETIRSLIWKLLGSMIPPEIQEKLSNSEEEY 120

Query: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180
           FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF
Sbjct: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQYISRGLMEELRG 201
           LKRTDAEQYISRGLMEELRG
Sbjct: 181 LKRTDAEQYISRGLMEELRG 200

[Close]

BLAST of Lsi02G027260 vs. NCBI nr
Match: XP_022964026.1 (probable DNA replication complex GINS protein PSF1 [Cucurbita moschata] >XP_023000395.1 probable DNA replication complex GINS protein PSF1 [Cucurbita maxima] >KAG6593434.1 DNA replication complex GINS protein PSF1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 386.0 bits (990), Expect = 2.0e-103
Identity = 190/200 (95.00%), Postives = 197/200 (98.50%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60
           MYGRKACQLVKELASGEKGQL  FNSDLFEQVISECQQHHLELQSL+RK+Q+EGLDLQTT
Sbjct: 1   MYGRKACQLVKELASGEKGQLAQFNSDLFEQVISECQQHHLELQSLLRKIQDEGLDLQTT 60

Query: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY 120
           KNEDHFGAL+HHLALVRNKRCLMAYV+NRAE IRSLIWKIVGSM+PPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALVHHLALVRNKRCLMAYVYNRAETIRSLIWKIVGSMLPPEIQEKLSNSEEEY 120

Query: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180
           FKKHSARLKEYMSK+ELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF
Sbjct: 121 FKKHSARLKEYMSKMELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQYISRGLMEELRG 201
           LKRTDAEQYISRGLMEELRG
Sbjct: 181 LKRTDAEQYISRGLMEELRG 200

[Close]

BLAST of Lsi02G027260 vs. NCBI nr
Match: XP_023514648.1 (probable DNA replication complex GINS protein PSF1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 383.3 bits (983), Expect = 1.3e-102
Identity = 189/200 (94.50%), Postives = 196/200 (98.00%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60
           MYGRKACQLVK LASGEKGQL  FNSDLFEQVISECQQHHLELQSL+RK+Q+EGLDLQTT
Sbjct: 1   MYGRKACQLVKVLASGEKGQLAQFNSDLFEQVISECQQHHLELQSLLRKIQDEGLDLQTT 60

Query: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY 120
           KNEDHFGAL+HHLALVRNKRCLMAYV+NRAE IRSLIWKIVGSM+PPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALVHHLALVRNKRCLMAYVYNRAETIRSLIWKIVGSMLPPEIQEKLSNSEEEY 120

Query: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180
           FKKHSARLKEYMSK+ELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF
Sbjct: 121 FKKHSARLKEYMSKMELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQYISRGLMEELRG 201
           LKRTDAEQYISRGLMEELRG
Sbjct: 181 LKRTDAEQYISRGLMEELRG 200

[Close]

BLAST of Lsi02G027260 vs. TAIR 10
Match: AT1G80190.1 (partner of SLD five 1 )

HSP 1 Score: 276.6 bits (706), Expect = 1.6e-74
Identity = 136/202 (67.33%), Postives = 164/202 (81.19%), Query Frame = 0


Query: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60
           MYGRK  QL+K+ A+GEKGQL  FNS LF++ I EC Q+H  +QSLIRK+Q+EGLD+Q  
Sbjct: 1   MYGRKGYQLIKDFATGEKGQLKPFNSKLFDETIEECDQNHHLIQSLIRKMQQEGLDVQNN 60

Query: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSM--IPPEIQEKLSNSEE 120
           +N DH+GALIHHLAL+RNKRCLMAYV+NRAEI+R L W++   +  +P EIQEKL+  E+
Sbjct: 61  RNADHYGALIHHLALIRNKRCLMAYVYNRAEIVRDLAWRVGLELLDLPSEIQEKLTTLEK 120

Query: 121 EYFKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSI 180
           EYFK HS  LK YM K+ ++L VDMVPPKDPYI+VR+LDDI EGIVLS DKT NFA HS+
Sbjct: 121 EYFKNHSVALKSYMGKVGIELNVDMVPPKDPYIKVRILDDIDEGIVLS-DKTTNFARHSM 180

Query: 181 HFLKRTDAEQYISRGLMEELRG 201
           HFLKRTDAE YI+RG MEEL G
Sbjct: 181 HFLKRTDAEPYIARGQMEELTG 201

The following BLAST results are available for this feature:

BLAST of Lsi02G027260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54HR6	2.0e-24	33.00	Probable DNA replication complex GINS protein PSF1 OS=Dictyostelium discoideum O...	[more]
Q7ZT47	4.8e-23	34.98	DNA replication complex GINS protein PSF1 OS=Xenopus laevis OX=8355 GN=gins1 PE=...	[more]
Q9CZ15	1.8e-22	35.96	DNA replication complex GINS protein PSF1 OS=Mus musculus OX=10090 GN=Gins1 PE=1...	[more]
A4IFH4	5.3e-22	34.98	DNA replication complex GINS protein PSF1 OS=Bos taurus OX=9913 GN=GINS1 PE=2 SV...	[more]
Q14691	7.0e-22	34.98	DNA replication complex GINS protein PSF1 OS=Homo sapiens OX=9606 GN=GINS1 PE=1 ...	[more]

BLAST of Lsi02G027260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K8U8	1.2e-106	98.50	Sld5 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G433190 PE=3 S...	[more]
A0A1S3CDY2	6.2e-106	98.00	probable DNA replication complex GINS protein PSF1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A5A7UU94	6.2e-106	98.00	Putative DNA replication complex GINS protein PSF1 OS=Cucumis melo var. makuwa O...	[more]
A0A6J1KI79	9.8e-104	95.00	probable DNA replication complex GINS protein PSF1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1HHQ1	9.8e-104	95.00	probable DNA replication complex GINS protein PSF1 OS=Cucurbita moschata OX=3662...	[more]

BLAST of Lsi02G027260 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897311.1	1.2e-106	99.00	probable DNA replication complex GINS protein PSF1 [Benincasa hispida]	[more]
XP_004135842.1	2.6e-106	98.50	probable DNA replication complex GINS protein PSF1 [Cucumis sativus] >KGN45289.1...	[more]
XP_008461089.1	1.3e-105	98.00	PREDICTED: probable DNA replication complex GINS protein PSF1 [Cucumis melo] >XP...	[more]
XP_022964026.1	2.0e-103	95.00	probable DNA replication complex GINS protein PSF1 [Cucurbita moschata] >XP_0230...	[more]
XP_023514648.1	1.3e-102	94.50	probable DNA replication complex GINS protein PSF1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi02G027260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80190.1	1.6e-74	67.33	partner of SLD five 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021151	GINS subunit, domain A	PFAM	PF05916	Sld5	coord: 48..117 e-value: 1.3E-6 score: 28.8
None	No IPR available	GENE3D	1.20.58.1030		coord: 1..135 e-value: 2.1E-39 score: 136.7
IPR005339	GINS complex, subunit Psf1	PANTHER	PTHR12914	PARTNER OF SLD5	coord: 1..196
IPR005339	GINS complex, subunit Psf1	CDD	cd11710	GINS_A_psf1	coord: 3..137 e-value: 8.37904E-47 score: 148.557
IPR036224	GINS, helical bundle-like domain superfamily	SUPERFAMILY	158573	GINS helical bundle-like	coord: 1..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G027260.1	Lsi02G027260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1902983	DNA strand elongation involved in mitotic DNA replication
biological_process	GO:0006260	DNA replication
cellular_component	GO:0000811	GINS complex