Lsi02G027070 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G027070
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	cucumber peeling cupredoxin-like
Location	chr02: 33444063 .. 33444317 (-)
RNA-Seq Expression	Lsi02G027070
Synteny	Lsi02G027070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGACAAGTCCGGTGACGATGAGACTCAACGACACAGGCATGAATTACTTTGTTTGCACTGTCGGCGCACATTGTTCGTCAGGTCAAAAGTTATCCATCAATGTCGTGGCCGCCACGGCTGGTGGTCCGATGTCGCCGACTTCACCCGGACCACCTTCTTCCTCACTACTACCGCCGCCTCGTTCCTCCTCCAATGCACTTACGGCAACCCTTTATCTCACTTTCTCCGCTCTTCTTATGGCCTTCTTTTAG

mRNA sequence

ATGACGACAAGTCCGGTGACGATGAGACTCAACGACACAGGCATGAATTACTTTGTTTGCACTGTCGGCGCACATTGTTCGTCAGGTCAAAAGTTATCCATCAATGTCGTGGCCGCCACGGCTGGTGGTCCGATGTCGCCGACTTCACCCGGACCACCTTCTTCCTCACTACTACCGCCGCCTCGTTCCTCCTCCAATGCACTTACGGCAACCCTTTATCTCACTTTCTCCGCTCTTCTTATGGCCTTCTTTTAG

Coding sequence (CDS)

ATGACGACAAGTCCGGTGACGATGAGACTCAACGACACAGGCATGAATTACTTTGTTTGCACTGTCGGCGCACATTGTTCGTCAGGTCAAAAGTTATCCATCAATGTCGTGGCCGCCACGGCTGGTGGTCCGATGTCGCCGACTTCACCCGGACCACCTTCTTCCTCACTACTACCGCCGCCTCGTTCCTCCTCCAATGCACTTACGGCAACCCTTTATCTCACTTTCTCCGCTCTTCTTATGGCCTTCTTTTAG

Protein sequence

MTTSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATAGGPMSPTSPGPPSSSLLPPPRSSSNALTATLYLTFSALLMAFF

Homology

[Close]

BLAST of Lsi02G027070 vs. ExPASy Swiss-Prot
Match: P29602 (Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3)

HSP 1 Score: 74.7 bits (182), Expect = 5.5e-13
Identity = 40/59 (67.80%), Postives = 46/59 (77.97%), Query Frame = 0


Query: 3   TSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATAGGPMSPTSPGPPSSSLLPPP 62
           TSPV  RL++ GM+YFVCTVG HCS+GQKLSINVVAA A   M P S  PP SS++PPP
Sbjct: 72  TSPVIERLDELGMHYFVCTVGTHCSNGQKLSINVVAANATVSMPPPSSSPP-SSVMPPP 129

[Close]

BLAST of Lsi02G027070 vs. ExPASy Swiss-Prot
Match: P42849 (Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.2e-07
Identity = 30/48 (62.50%), Postives = 34/48 (70.83%), Query Frame = 0


Query: 1   MTTSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVV-AATAGGPMSP 48
           MTT PV + LN TG  Y++CTVG HC  GQKLSINVV A  AGG  +P
Sbjct: 66  MTTPPVKIMLNTTGPQYYICTVGDHCRVGQKLSINVVGAGGAGGGATP 113

[Close]

BLAST of Lsi02G027070 vs. ExPASy Swiss-Prot
Match: Q07488 (Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 1.6e-07
Identity = 31/59 (52.54%), Postives = 36/59 (61.02%), Query Frame = 0


Query: 1   MTTSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATAGGPMSPTSPGPPSSSLLP 60
           MT  PV + LN TG  YF+CTVG HC  GQKLSI VVAA A G  +P +   P+    P
Sbjct: 88  MTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLSITVVAAGATGGATPGAGATPAPGSTP 146

[Close]

BLAST of Lsi02G027070 vs. ExPASy Swiss-Prot
Match: O80517 (Uclacyanin-2 OS=Arabidopsis thaliana OX=3702 GN=At2g44790 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 5.5e-05
Identity = 28/68 (41.18%), Postives = 39/68 (57.35%), Query Frame = 0


Query: 10  LNDTGMNYFVCTVGAHC--SSGQKLSINVVAATAGGPMSPT----SPGPPSSSLLPPPRS 69
           L   G+NYF+C+   HC  + G KL++NVVA +AG P +PT    +PG P++   PP   
Sbjct: 96  LKTVGINYFICSTPGHCRTNGGMKLAVNVVAGSAGPPATPTPPSSTPGTPTTPESPPSGG 155

Query: 70  SSNALTAT 72
           S    T T
Sbjct: 156 SPTPTTPT 163

[Close]

BLAST of Lsi02G027070 vs. ExPASy TrEMBL
Match: A0A5D3CF84 (Cucumber peeling cupredoxin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001960 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 9.1e-19
Identity = 61/89 (68.54%), Postives = 68/89 (76.40%), Query Frame = 0


Query: 3   TSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATA------GGPMSPTSPGPPSSS 62
           T PV  RL++ GM+YFVCTVG HCSSGQKL+INVV+ATA      GGPMSP+SP PPSS 
Sbjct: 94  TGPVIERLDELGMHYFVCTVGTHCSSGQKLAINVVSATAGGPMSPGGPMSPSSPSPPSSV 153

Query: 63  LLPPPRSSSNALTATLYLTFSALL-MAFF 85
           + PP  SSSNAL ATL LTF ALL M FF
Sbjct: 154 MPPPSPSSSNALMATLPLTFFALLVMTFF 182

[Close]

BLAST of Lsi02G027070 vs. ExPASy TrEMBL
Match: A0A5A7UZ00 (Cucumber peeling cupredoxin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G002210 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 9.1e-19
Identity = 61/89 (68.54%), Postives = 68/89 (76.40%), Query Frame = 0


Query: 3   TSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATA------GGPMSPTSPGPPSSS 62
           T PV  RL++ GM+YFVCTVG HCSSGQKL+INVV+ATA      GGPMSP+SP PPSS 
Sbjct: 391 TGPVIERLDELGMHYFVCTVGTHCSSGQKLAINVVSATAGGPMSPGGPMSPSSPSPPSSV 450

Query: 63  LLPPPRSSSNALTATLYLTFSALL-MAFF 85
           + PP  SSSNAL ATL LTF ALL M FF
Sbjct: 451 MPPPSPSSSNALMATLPLTFFALLVMTFF 479

[Close]

BLAST of Lsi02G027070 vs. ExPASy TrEMBL
Match: A0A1S3CDJ1 (cucumber peeling cupredoxin-like OS=Cucumis melo OX=3656 GN=LOC103499805 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 9.1e-19
Identity = 61/89 (68.54%), Postives = 68/89 (76.40%), Query Frame = 0


Query: 3   TSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATA------GGPMSPTSPGPPSSS 62
           T PV  RL++ GM+YFVCTVG HCSSGQKL+INVV+ATA      GGPMSP+SP PPSS 
Sbjct: 94  TGPVIERLDELGMHYFVCTVGTHCSSGQKLAINVVSATAGGPMSPGGPMSPSSPSPPSSV 153

Query: 63  LLPPPRSSSNALTATLYLTFSALL-MAFF 85
           + PP  SSSNAL ATL LTF ALL M FF
Sbjct: 154 MPPPSPSSSNALMATLPLTFFALLVMTFF 182

[Close]

BLAST of Lsi02G027070 vs. ExPASy TrEMBL
Match: Q96403 (Stellacyanin OS=Cucumis sativus OX=3659 GN=Csa_7G432470 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.0e-17
Identity = 62/88 (70.45%), Postives = 68/88 (77.27%), Query Frame = 0


Query: 3   TSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATAGGPMSPTSPGPPS----SSLL 62
           TSPV  RL++ GM+YFVCTVG HCS+GQKLSINVVAA A   M P S  PPS    SS++
Sbjct: 95  TSPVIERLDELGMHYFVCTVGTHCSNGQKLSINVVAANATVSMPPPSSSPPSSSPPSSVM 154

Query: 63  PPPR-SSSNALTATLYLTFSALL-MAFF 85
           PPP  SSSNAL ATLYLTFSALL MAFF
Sbjct: 155 PPPSPSSSNALMATLYLTFSALLVMAFF 182

[Close]

BLAST of Lsi02G027070 vs. ExPASy TrEMBL
Match: A0A6J1GPD3 (cucumber peeling cupredoxin-like OS=Cucurbita moschata OX=3662 GN=LOC111456235 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.0e-17
Identity = 53/84 (63.10%), Postives = 63/84 (75.00%), Query Frame = 0


Query: 1   MTTSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATAGGPMSPTSPGPPSSSLLPP 60
           MTT P T+ LN TGM+YF+CT+G HCS GQK++INV A  AGGPMSP  P   S    PP
Sbjct: 95  MTTGPATVALNSTGMHYFICTIGTHCSLGQKVAINVGATAAGGPMSPPMPASQSP---PP 154

Query: 61  PRSSSNALTATLYLTFSALLMAFF 85
             SS+NA+TATLYLTFSA++MA F
Sbjct: 155 SPSSANAVTATLYLTFSAVVMALF 175

[Close]

BLAST of Lsi02G027070 vs. NCBI nr
Match: XP_038896386.1 (cucumber peeling cupredoxin-like [Benincasa hispida])

HSP 1 Score: 112.5 bits (280), Expect = 1.8e-21
Identity = 58/80 (72.50%), Postives = 68/80 (85.00%), Query Frame = 0


Query: 5   PVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATAGGPMSPTSPGPPSSSLLPPPRSS 64
           P T+ LNDT M+YFVCTVG HCS GQKLSINVV+A AGGP+SP+SP PPS+ L PP  SS
Sbjct: 96  PATVTLNDTEMHYFVCTVGPHCSLGQKLSINVVSAAAGGPISPSSPAPPSTVLPPPSPSS 155

Query: 65  SNALTATLYLTFSALLMAFF 85
           SN+L+ATLYL+FSA+LM FF
Sbjct: 156 SNSLSATLYLSFSAILMTFF 175

[Close]

BLAST of Lsi02G027070 vs. NCBI nr
Match: XP_008461124.1 (PREDICTED: cucumber peeling cupredoxin-like [Cucumis melo] >TYK10543.1 cucumber peeling cupredoxin-like [Cucumis melo var. makuwa])

HSP 1 Score: 102.4 bits (254), Expect = 1.9e-18
Identity = 61/89 (68.54%), Postives = 68/89 (76.40%), Query Frame = 0


Query: 3   TSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATA------GGPMSPTSPGPPSSS 62
           T PV  RL++ GM+YFVCTVG HCSSGQKL+INVV+ATA      GGPMSP+SP PPSS 
Sbjct: 94  TGPVIERLDELGMHYFVCTVGTHCSSGQKLAINVVSATAGGPMSPGGPMSPSSPSPPSSV 153

Query: 63  LLPPPRSSSNALTATLYLTFSALL-MAFF 85
           + PP  SSSNAL ATL LTF ALL M FF
Sbjct: 154 MPPPSPSSSNALMATLPLTFFALLVMTFF 182

[Close]

BLAST of Lsi02G027070 vs. NCBI nr
Match: KAA0058749.1 (cucumber peeling cupredoxin-like [Cucumis melo var. makuwa])

HSP 1 Score: 102.4 bits (254), Expect = 1.9e-18
Identity = 61/89 (68.54%), Postives = 68/89 (76.40%), Query Frame = 0


Query: 3   TSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATA------GGPMSPTSPGPPSSS 62
           T PV  RL++ GM+YFVCTVG HCSSGQKL+INVV+ATA      GGPMSP+SP PPSS 
Sbjct: 391 TGPVIERLDELGMHYFVCTVGTHCSSGQKLAINVVSATAGGPMSPGGPMSPSSPSPPSSV 450

Query: 63  LLPPPRSSSNALTATLYLTFSALL-MAFF 85
           + PP  SSSNAL ATL LTF ALL M FF
Sbjct: 451 MPPPSPSSSNALMATLPLTFFALLVMTFF 479

[Close]

BLAST of Lsi02G027070 vs. NCBI nr
Match: KAG6575597.1 (hypothetical protein SDJN03_26236, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 100.5 bits (249), Expect = 7.2e-18
Identity = 54/84 (64.29%), Postives = 64/84 (76.19%), Query Frame = 0


Query: 1   MTTSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATAGGPMSPTSPGPPSSSLLPP 60
           MTT PVT+ LN TGM+YF+CT+G HCS GQK++INV A  AGGPMSP  P   S    PP
Sbjct: 95  MTTGPVTVALNSTGMHYFICTIGTHCSLGQKVAINVGATAAGGPMSPPMPASQSP---PP 154

Query: 61  PRSSSNALTATLYLTFSALLMAFF 85
             SS+NA+TATLYLTFSA++MA F
Sbjct: 155 SPSSANAVTATLYLTFSAVVMALF 175

[Close]

BLAST of Lsi02G027070 vs. NCBI nr
Match: XP_023547709.1 (cucumber peeling cupredoxin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 100.1 bits (248), Expect = 9.4e-18
Identity = 54/84 (64.29%), Postives = 64/84 (76.19%), Query Frame = 0


Query: 1   MTTSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATAGGPMSPTSPGPPSSSLLPP 60
           MTT PVT+ LN TGM+YF+CT+G HCS GQK++INV A  AGGPMSP  P   S    PP
Sbjct: 96  MTTGPVTVALNSTGMHYFICTIGTHCSLGQKVAINVGATAAGGPMSPPVPASQSP---PP 155

Query: 61  PRSSSNALTATLYLTFSALLMAFF 85
             SS+NA+TATLYLTFSA++MA F
Sbjct: 156 SPSSANAVTATLYLTFSAVVMALF 176

[Close]

BLAST of Lsi02G027070 vs. TAIR 10
Match: AT5G20230.1 (blue-copper-binding protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.1e-08
Identity = 31/59 (52.54%), Postives = 36/59 (61.02%), Query Frame = 0


Query: 1   MTTSPVTMRLNDTGMNYFVCTVGAHCSSGQKLSINVVAATAGGPMSPTSPGPPSSSLLP 60
           MT  PV + LN TG  YF+CTVG HC  GQKLSI VVAA A G  +P +   P+    P
Sbjct: 88  MTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLSITVVAAGATGGATPGAGATPAPGSTP 146

[Close]

BLAST of Lsi02G027070 vs. TAIR 10
Match: AT2G44790.1 (uclacyanin 2 )

HSP 1 Score: 48.1 bits (113), Expect = 3.9e-06
Identity = 28/68 (41.18%), Postives = 39/68 (57.35%), Query Frame = 0


Query: 10  LNDTGMNYFVCTVGAHC--SSGQKLSINVVAATAGGPMSPT----SPGPPSSSLLPPPRS 69
           L   G+NYF+C+   HC  + G KL++NVVA +AG P +PT    +PG P++   PP   
Sbjct: 96  LKTVGINYFICSTPGHCRTNGGMKLAVNVVAGSAGPPATPTPPSSTPGTPTTPESPPSGG 155

Query: 70  SSNALTAT 72
           S    T T
Sbjct: 156 SPTPTTPT 163

The following BLAST results are available for this feature:

BLAST of Lsi02G027070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P29602	5.5e-13	67.80	Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3	[more]
P42849	1.2e-07	62.50	Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1	[more]
Q07488	1.6e-07	52.54	Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2	[more]
O80517	5.5e-05	41.18	Uclacyanin-2 OS=Arabidopsis thaliana OX=3702 GN=At2g44790 PE=2 SV=1	[more]

BLAST of Lsi02G027070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CF84	9.1e-19	68.54	Cucumber peeling cupredoxin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7UZ00	9.1e-19	68.54	Cucumber peeling cupredoxin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A1S3CDJ1	9.1e-19	68.54	cucumber peeling cupredoxin-like OS=Cucumis melo OX=3656 GN=LOC103499805 PE=4 SV...	[more]
Q96403	1.0e-17	70.45	Stellacyanin OS=Cucumis sativus OX=3659 GN=Csa_7G432470 PE=2 SV=1	[more]
A0A6J1GPD3	1.0e-17	63.10	cucumber peeling cupredoxin-like OS=Cucurbita moschata OX=3662 GN=LOC111456235 P...	[more]

BLAST of Lsi02G027070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896386.1	1.8e-21	72.50	cucumber peeling cupredoxin-like [Benincasa hispida]	[more]
XP_008461124.1	1.9e-18	68.54	PREDICTED: cucumber peeling cupredoxin-like [Cucumis melo] >TYK10543.1 cucumber ...	[more]
KAA0058749.1	1.9e-18	68.54	cucumber peeling cupredoxin-like [Cucumis melo var. makuwa]	[more]
KAG6575597.1	7.2e-18	64.29	hypothetical protein SDJN03_26236, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023547709.1	9.4e-18	64.29	cucumber peeling cupredoxin-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi02G027070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20230.1	1.1e-08	52.54	blue-copper-binding protein	[more]
AT2G44790.1	3.9e-06	41.18	uclacyanin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 1..46 e-value: 5.8E-10 score: 41.2
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 4..38
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 42..64
None	No IPR available	PANTHER	PTHR33021:SF302	BLUE COPPER PROTEIN	coord: 1..72
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 1..72
IPR028871	Blue (type 1) copper protein, binding site	PROSITE	PS00196	COPPER_BLUE	coord: 14..30
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 1..38 score: 16.294031

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G027070.1	Lsi02G027070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0009055	electron transfer activity