Lsi02G026730 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G026730
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	deSI-like protein At4g17486
Location	chr02: 33202273 .. 33204708 (+)
RNA-Seq Expression	Lsi02G026730
Synteny	Lsi02G026730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTATACTATATATACTTAGCTAGCACGAGAAAATTAAGGGAGGCAGCTAAAAAGCCCTCAGTGGCAATTGATGTAATTTGAGCCCAAAACCAAGCCTGATGATATTCCCTCACCAAACTAGAATCATCCACGGTCCAGACCATATCTACGGTGTTCAACAACGAGTCGCCGCCGGTGACGCCCGTTAGAATTGCAAACTAAGAAACGATAGTTTCCATTCATCTCCATAGATTTTGAATTTCAAATTTTCCATATCTCGAGGACTTCACTCTGTGTTTTTCCTGCTCCAACTTTTTGGGACTTCTTGGCCATGAATTGAGGCGGAATGATGTTGTTGCTTAAGAAATCAATCGGAAAAACCACCGGAGGATCCGTGCCGGTGTACCTGAATGTGTACGATCTAACCGCGATTAATGGCTATGCTTACTGGTTTGGCCTTGGCGTTTTCCATTCCGGAGTTCAAGGTCAGTAATGGCAGTAATTGCTTCCGTTTGATCTTTGTTTCCAAAGGCGTTTCGTGAGTTTTTCTGTTTTCTCTTGGCCGAAGCTCTAATGCTGCTTTTGCGTTTTTCTTATTTTTTATTTTCTTTTTTGTGATCTTGCTAATTCGTTGATGCGATGCGATTTGCGACATTTTTTTGTTTGTTTTGATTTGTGTTTGGATAGGAAGAAGGTTTCAGTGACTGTTAATTGTGTGTACTGTACTGTGAATAGAGAGATGCATCGGCTGTGAGAAAAATTGTTCTGATTAGCCAAACGCTTGTTCATGAACTATCAGTTCGTTTTACGGCCCGTTTTTATCTCGATTCAGCAATTTTTCTGTTAATTATACTACTGATTAAGCTATCGAATTGCGAGCGTTTTTTAGGTTTGGCAGATGAGTAAATTTTCTGACAAGCCAAACACTCATTTCTGATTTCGATGCTATTTTCAGCTTTTCGTCTGAATGAACCAGCTCGTTTCCTTTAATAGTATGTTGATTTCGTAGTCGATGCCGGTGGAGTTCTTATGCTCAATTTGGCAGATCCTTCAGTTGAATTTCCGGAACTCGATTTGACATTTTTGCTGTTGATTTCGATTGTGTAGTTCATGGTGTGGAATACGCATTTGGAGCTCACGAGTACTCAACAACGGGAATTTTTGAAGGAGTGCCGAAGCAATGCGACGGTTTTAGATTTAGGAAGACAGTTTTGGTAGGCAAAACAGATATGAAACCAACAGAGGTGAGAGCTTTAATGGAGGAACTTTCTCAAATCTACAAGGGAAATGCTTACAATTTAATCACCAAAAATTGCAACCATTTTTGCAATGATGCTTGTATTAAACTCACAGGGAATTCCATCCCAAATTGGGTTAATCGCCTTGCTAGAATTGGTAAGTAAGAAACTACCTCTTTCTTTTATCCATTTGTGGCTTGTTTAGCATATTAAATTCCTTTTCAGTATTTGCATTTGTACTGATTTCAAGTTCATTTAAGAGGTTAACATAATTTTCTGATGAATATGAACACAACCCAAATTGAGCTTTGGGGTTCATTTCTTTTCTTGCTTGTGAGAATCTTAAGTACTTTAGTAGCAAGATCATATTCATCATCAGCTTGCTTTGAAATTGTTTGAGGAGGCGGCCATAATTAGGCGGGTAGTTTTGCTTTGTTGGATGTATGAGAAGAAACTACAAAAGTACTTTGAATTTGTATCGATATACAAATTTTCAAATCCACATCCAACAGTAGTCACATTATAGCAAACAATTTTTCTTGCTTGGTTTTGTGCCACCCCATTTTGGATCAATGGTTTTTTTTTTCCTTCTCCTTTTTCAGGCTTTCTTTGTAATTGTGTTCTACCAGTGACCTTCAATTCAACCAAAATCCGGCACCACCACAGAATTGAAGACAAGGTTGTAACCATGGAGACAAAGAAGGAACTAACAAGTGAATCAACAAAAACTCCCAGTTCTAATTCCTCTTCATCTGCAACTTCTTCTCCTTGCTTGACGTTTCGCCGAGGAAGAAGTCGGACGAGATGAGCTCCTCCTCCATCTTCTCCATTGTTTTTTCATTCTTCGTCCTCATCTTCATGAGGGACAGACATGATTGGAACCTCAGCTCCTTAGATTTTTTTGTCATTTCCATTGGAAGTGCTCAAAGAAGCTGAAACAGTCACAATTCTCATCTTTTCCTCTGTTATCTTCTTCTTAAAGATCTTGGAAGGTCAATTTCAAATCAGAAGCCATTGATTTGTATGCTTTCGATTCCCTTCTTTTGCCTCTTAATTGTTTTCCTGTATTATTTCTATTTCTTTTCTTTTCTTTTTGCATTCATCACCTTTGAATGAATGCTCCCAAGGAACAATTCCAAGTTGTAAAAGTTGAAATTATTGTGAAAGTTAAAATCACAATTCATATTCTCATAATTTAAATAAAGTGTAGGTTTTTGTT

mRNA sequence

TTATACTATATATACTTAGCTAGCACGAGAAAATTAAGGGAGGCAGCTAAAAAGCCCTCAGTGGCAATTGATGTAATTTGAGCCCAAAACCAAGCCTGATGATATTCCCTCACCAAACTAGAATCATCCACGGTCCAGACCATATCTACGGTGTTCAACAACGAGTCGCCGCCGGTGACGCCCGTTAGAATTGCAAACTAAGAAACGATAGTTTCCATTCATCTCCATAGATTTTGAATTTCAAATTTTCCATATCTCGAGGACTTCACTCTGTGTTTTTCCTGCTCCAACTTTTTGGGACTTCTTGGCCATGAATTGAGGCGGAATGATGTTGTTGCTTAAGAAATCAATCGGAAAAACCACCGGAGGATCCGTGCCGGTGTACCTGAATGTGTACGATCTAACCGCGATTAATGGCTATGCTTACTGGTTTGGCCTTGGCGTTTTCCATTCCGGAGTTCAAGTTCATGGTGTGGAATACGCATTTGGAGCTCACGAGTACTCAACAACGGGAATTTTTGAAGGAGTGCCGAAGCAATGCGACGGTTTTAGATTTAGGAAGACAGTTTTGGTAGGCAAAACAGATATGAAACCAACAGAGGGAATTCCATCCCAAATTGGGTTAATCGCCTTGCTAGAATTGGTAAGCTTTCTTTGTAATTGTGTTCTACCAGTGACCTTCAATTCAACCAAAATCCGGCACCACCACAGAATTGAAGACAAGGTTGTAACCATGGAGACAAAGAAGGAACTAACAAGTGAATCAACAAAAACTCCCAGTTCTAATTCCTCTTCATCTGCAACTTCTTCTCCTTGCTTGACGTTTCGCCGAGGAAGAAGTCGGACGAGATGAGCTCCTCCTCCATCTTCTCCATTGTTTTTTCATTCTTCGTCCTCATCTTCATGAGGGACAGACATGATTGGAACCTCAGCTCCTTAGATTTTTTTGTCATTTCCATTGGAAGTGCTCAAAGAAGCTGAAACAGTCACAATTCTCATCTTTTCCTCTGTTATCTTCTTCTTAAAGATCTTGGAAGGTCAATTTCAAATCAGAAGCCATTGATTTGTATGCTTTCGATTCCCTTCTTTTGCCTCTTAATTGTTTTCCTGTATTATTTCTATTTCTTTTCTTTTCTTTTTGCATTCATCACCTTTGAATGAATGCTCCCAAGGAACAATTCCAAGTTGTAAAAGTTGAAATTATTGTGAAAGTTAAAATCACAATTCATATTCTCATAATTTAAATAAAGTGTAGGTTTTTGTT

Coding sequence (CDS)

ATGATGTTGTTGCTTAAGAAATCAATCGGAAAAACCACCGGAGGATCCGTGCCGGTGTACCTGAATGTGTACGATCTAACCGCGATTAATGGCTATGCTTACTGGTTTGGCCTTGGCGTTTTCCATTCCGGAGTTCAAGTTCATGGTGTGGAATACGCATTTGGAGCTCACGAGTACTCAACAACGGGAATTTTTGAAGGAGTGCCGAAGCAATGCGACGGTTTTAGATTTAGGAAGACAGTTTTGGTAGGCAAAACAGATATGAAACCAACAGAGGGAATTCCATCCCAAATTGGGTTAATCGCCTTGCTAGAATTGGTAAGCTTTCTTTGTAATTGTGTTCTACCAGTGACCTTCAATTCAACCAAAATCCGGCACCACCACAGAATTGAAGACAAGGTTGTAACCATGGAGACAAAGAAGGAACTAACAAGTGAATCAACAAAAACTCCCAGTTCTAATTCCTCTTCATCTGCAACTTCTTCTCCTTGCTTGACGTTTCGCCGAGGAAGAAGTCGGACGAGATGA

Protein sequence

MMLLLKKSIGKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYSTTGIFEGVPKQCDGFRFRKTVLVGKTDMKPTEGIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKELTSESTKTPSSNSSSSATSSPCLTFRRGRSRTR

Homology

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BLAST of Lsi02G026730 vs. ExPASy Swiss-Prot
Match: Q93VG8 (DeSI-like protein At4g17486 OS=Arabidopsis thaliana OX=3702 GN=At4g17486 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.5e-23
Identity = 43/75 (57.33%), Postives = 59/75 (78.67%), Query Frame = 0


Query: 18  PVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYSTTGIFEGVPKQCDGFRF 77
           PVYLNVYDLT +N Y YWFG+G+FHSG++ H +EY +GAHEY T+G++E  P+ C GF F
Sbjct: 27  PVYLNVYDLTPVNNYLYWFGIGIFHSGIEAHNLEYCYGAHEYPTSGVYEVEPRNCPGFIF 86

Query: 78  RKTVLVGKTDMKPTE 93
           R++VL+G T M  ++
Sbjct: 87  RRSVLLGTTSMSRSD 101

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BLAST of Lsi02G026730 vs. ExPASy Swiss-Prot
Match: H2KZK4 (Desumoylating isopeptidase 1 homolog OS=Caenorhabditis elegans OX=6239 GN=F36D4.5 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.0e-13
Identity = 33/78 (42.31%), Postives = 54/78 (69.23%), Query Frame = 0


Query: 19  VYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYSTTGIFEGVPKQC----DG 78
           V LNVYD+  +N YA   G+G+FHSG++V GVEYA+G H Y  +G+FE  P+      + 
Sbjct: 32  VRLNVYDMYWLNDYASNIGVGIFHSGIEVFGVEYAYGGHPYQFSGVFENSPQDAEELGET 91

Query: 79  FRFRKTVLVGKTDMKPTE 93
           F+F+++++VG+T+   ++
Sbjct: 92  FKFKESIVVGETERSTSD 109

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BLAST of Lsi02G026730 vs. ExPASy Swiss-Prot
Match: Q5PQ09 (Deubiquitinase DESI2 OS=Xenopus laevis OX=8355 GN=desi2 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.3e-12
Identity = 34/74 (45.95%), Postives = 48/74 (64.86%), Query Frame = 0


Query: 18 PVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYSTTGIFEGVPKQC----D 77
          P+ LNVYD+  IN Y    G+GVFHSG+QV+G E+A+G H Y  +G+FE  P       D
Sbjct: 5  PIILNVYDMYWINEYTSSLGIGVFHSGIQVYGREFAYGGHPYPFSGVFEISPGDSTELGD 64

Query: 78 GFRFRKTVLVGKTD 88
           F+F++ + +G TD
Sbjct: 65 TFKFKEAIALGSTD 78

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BLAST of Lsi02G026730 vs. ExPASy Swiss-Prot
Match: Q6DC39 (Deubiquitinase DESI2 OS=Danio rerio OX=7955 GN=desi2 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 5.7e-12
Identity = 35/88 (39.77%), Postives = 55/88 (62.50%), Query Frame = 0


Query: 18  PVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYSTTGIFEGVPKQC----D 77
           PV LNVYD+  IN +    G+GVFHSG++++G E+A+G H Y  +GIFE  P       +
Sbjct: 5   PVILNVYDMYWINEFTSSLGIGVFHSGIEIYGREFAYGGHPYPFSGIFEITPGDATELGE 64

Query: 78  GFRFRKTVLVGKTDM--KPTEGIPSQIG 100
            F+F++ +++G TD   +  E I  ++G
Sbjct: 65  TFKFKEAIVLGSTDFTEEDVERIVEEMG 92

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BLAST of Lsi02G026730 vs. ExPASy Swiss-Prot
Match: Q9BSY9 (Deubiquitinase DESI2 OS=Homo sapiens OX=9606 GN=DESI2 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.8e-11
Identity = 34/78 (43.59%), Postives = 50/78 (64.10%), Query Frame = 0


Query: 14 GGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYSTTGIFEGVPKQC- 73
          G +  V LNVYD+  +N Y    G+GVFHSG++V+G E+A+G H Y  +GIFE  P    
Sbjct: 2  GANQLVVLNVYDMYWMNEYTSSIGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNAS 61

Query: 74 ---DGFRFRKTVLVGKTD 88
             + F+F++ V++G TD
Sbjct: 62 ELGETFKFKEAVVLGSTD 79

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BLAST of Lsi02G026730 vs. ExPASy TrEMBL
Match: A0A5A7UYX3 (DeSI-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001770 PE=3 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 6.7e-72
Identity = 151/213 (70.89%), Postives = 160/213 (75.12%), Query Frame = 0


Query: 1   MMLLLKKSIGKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYS 60
           M+ LLKK IGKTTGGSVPVYLNVYDLTAINGYAYW GLGVFHSGVQVHGVEYAFGAHEYS
Sbjct: 1   MVFLLKKPIGKTTGGSVPVYLNVYDLTAINGYAYWLGLGVFHSGVQVHGVEYAFGAHEYS 60

Query: 61  TTGIFEGVPKQCDGFRFRKTVLVGKTDMKPTE---------------------------- 120
           TTGIFEGVPKQCDGFRFRKT+LVGKTDMKPTE                            
Sbjct: 61  TTGIFEGVPKQCDGFRFRKTILVGKTDMKPTEVRALMEELAQIYKGNAYNLITKNCNHFC 120

Query: 121 ----------GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKE 176
                      IP+ +  +A    + FLCNCVLPVT NST+IRHH RIEDKVVTMETKKE
Sbjct: 121 NDACIKLTGNSIPNWVNRLA---RIGFLCNCVLPVTLNSTRIRHHQRIEDKVVTMETKKE 180

[Close]

BLAST of Lsi02G026730 vs. ExPASy TrEMBL
Match: A0A1S3CEJ5 (deSI-like protein At4g17486 OS=Cucumis melo OX=3656 GN=LOC103499833 PE=3 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 5.6e-71
Identity = 149/213 (69.95%), Postives = 159/213 (74.65%), Query Frame = 0


Query: 1   MMLLLKKSIGKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYS 60
           M+ LLKK IGKTTGGSVPVYLNVYDLTAINGYAYW GLGVFHSGVQVHGVEYAFGAHEYS
Sbjct: 1   MVFLLKKPIGKTTGGSVPVYLNVYDLTAINGYAYWLGLGVFHSGVQVHGVEYAFGAHEYS 60

Query: 61  TTGIFEGVPKQCDGFRFRKTVLVGKTDMKPTE---------------------------- 120
           TTGIFEG+PKQCDGFRFRKT+LVGKTDMKPTE                            
Sbjct: 61  TTGIFEGMPKQCDGFRFRKTILVGKTDMKPTEVRALMEELAQIYKGNAYNLITKNCNHFC 120

Query: 121 ----------GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKE 176
                      IP+ +  +A    + FLCNCVLPVT NST+IRHH RIEDKVVTMET KE
Sbjct: 121 NDACIKLTGNSIPNWVNRLA---RIGFLCNCVLPVTLNSTRIRHHQRIEDKVVTMETNKE 180

[Close]

BLAST of Lsi02G026730 vs. ExPASy TrEMBL
Match: A0A0A0KBP9 (PPPDE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G432070 PE=3 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 9.6e-71
Identity = 150/213 (70.42%), Postives = 158/213 (74.18%), Query Frame = 0


Query: 1   MMLLLKKSIGKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYS 60
           M+ LLKK IGKTTGGSVPVYLNVYDLTAINGYAYW GLGVFHSGVQVHGVEYAFGAHEYS
Sbjct: 1   MVFLLKKPIGKTTGGSVPVYLNVYDLTAINGYAYWLGLGVFHSGVQVHGVEYAFGAHEYS 60

Query: 61  TTGIFEGVPKQCDGFRFRKTVLVGKTDMKPTE---------------------------- 120
           TTGIFEGVPKQCDGFRFRKT+LVGKTDMKPTE                            
Sbjct: 61  TTGIFEGVPKQCDGFRFRKTILVGKTDMKPTEVRSLMEELAQIYKGNAYNLITKNCNHFC 120

Query: 121 ----------GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKE 176
                      IP+ +  +A    + FLCNCVLPVT NST+IRHHHRIEDKVVTME KKE
Sbjct: 121 NDACIKLTGNSIPNWVNRLA---RIGFLCNCVLPVTLNSTRIRHHHRIEDKVVTMEAKKE 180

[Close]

BLAST of Lsi02G026730 vs. ExPASy TrEMBL
Match: A0A6J1H916 (deSI-like protein At4g17486 OS=Cucurbita moschata OX=3662 GN=LOC111460737 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 8.1e-70
Identity = 146/212 (68.87%), Postives = 158/212 (74.53%), Query Frame = 0


Query: 2   MLLLKKSIGKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYST 61
           MLL KK +GK TGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYST
Sbjct: 1   MLLFKKPVGKQTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYST 60

Query: 62  TGIFEGVPKQCDGFRFRKTVLVGKTDMKPTE----------------------------- 121
           TGIFEGVPKQC GFRFRK+VL+GKTDMKP+E                             
Sbjct: 61  TGIFEGVPKQCHGFRFRKSVLIGKTDMKPSEVRALMEELAQIYKGNAYNLITKNCNHFCN 120

Query: 122 ---------GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKEL 176
                     IP+ +  +A    + FLCNCVLPVTFNST+IRHHHRIEDKVVTMETKK+L
Sbjct: 121 DACVKLTENSIPNWVNRLA---RIGFLCNCVLPVTFNSTRIRHHHRIEDKVVTMETKKDL 180

[Close]

BLAST of Lsi02G026730 vs. ExPASy TrEMBL
Match: A0A6J1KRJ3 (deSI-like protein At4g17486 OS=Cucurbita maxima OX=3661 GN=LOC111498027 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 3.4e-68
Identity = 144/212 (67.92%), Postives = 155/212 (73.11%), Query Frame = 0


Query: 2   MLLLKKSIGKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYST 61
           MLL KK +GK TGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYST
Sbjct: 1   MLLFKKPVGKQTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYST 60

Query: 62  TGIFEGVPKQCDGFRFRKTVLVGKTDMKPTE----------------------------- 121
           TGIFEGVPKQC GFRFRK+VL+GKTDMKP E                             
Sbjct: 61  TGIFEGVPKQCHGFRFRKSVLIGKTDMKPAEVRALMEELAQIYKGNAYNLITKNCNHFCN 120

Query: 122 ---------GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKEL 176
                     IP+ +  +A    + FLCNCVLPVTFNST+IRHHHRIE KVVTMETKK+L
Sbjct: 121 DACVKLTENSIPNWVNRLA---RIGFLCNCVLPVTFNSTRIRHHHRIEHKVVTMETKKDL 180

[Close]

BLAST of Lsi02G026730 vs. NCBI nr
Match: KAA0058719.1 (deSI-like protein [Cucumis melo var. makuwa] >TYK10526.1 deSI-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 280.0 bits (715), Expect = 1.4e-71
Identity = 151/213 (70.89%), Postives = 160/213 (75.12%), Query Frame = 0


Query: 1   MMLLLKKSIGKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYS 60
           M+ LLKK IGKTTGGSVPVYLNVYDLTAINGYAYW GLGVFHSGVQVHGVEYAFGAHEYS
Sbjct: 1   MVFLLKKPIGKTTGGSVPVYLNVYDLTAINGYAYWLGLGVFHSGVQVHGVEYAFGAHEYS 60

Query: 61  TTGIFEGVPKQCDGFRFRKTVLVGKTDMKPTE---------------------------- 120
           TTGIFEGVPKQCDGFRFRKT+LVGKTDMKPTE                            
Sbjct: 61  TTGIFEGVPKQCDGFRFRKTILVGKTDMKPTEVRALMEELAQIYKGNAYNLITKNCNHFC 120

Query: 121 ----------GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKE 176
                      IP+ +  +A    + FLCNCVLPVT NST+IRHH RIEDKVVTMETKKE
Sbjct: 121 NDACIKLTGNSIPNWVNRLA---RIGFLCNCVLPVTLNSTRIRHHQRIEDKVVTMETKKE 180

[Close]

BLAST of Lsi02G026730 vs. NCBI nr
Match: XP_038899104.1 (deSI-like protein At4g17486 [Benincasa hispida])

HSP 1 Score: 277.3 bits (708), Expect = 8.9e-71
Identity = 152/213 (71.36%), Postives = 159/213 (74.65%), Query Frame = 0


Query: 1   MMLLLKKSIGKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYS 60
           MM LLKK IGKTT GSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYS
Sbjct: 1   MMFLLKKPIGKTTRGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYS 60

Query: 61  TTGIFEGVPKQCDGFRFRKTVLVGKTDMKPTE---------------------------- 120
           TTGIFEGVPKQCDGFRFRKTVLVGKTDMKPTE                            
Sbjct: 61  TTGIFEGVPKQCDGFRFRKTVLVGKTDMKPTEVRALMEELAQIYKGNAYNLITKNCNHFC 120

Query: 121 ----------GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKE 176
                      IP+ +  +A    + FLCNCVLPVT NST+IRHH+RIEDKV+TMETKKE
Sbjct: 121 NDACIKLTGNSIPNWVNRLA---RIGFLCNCVLPVTLNSTRIRHHNRIEDKVITMETKKE 180

[Close]

BLAST of Lsi02G026730 vs. NCBI nr
Match: XP_008461171.1 (PREDICTED: deSI-like protein At4g17486 [Cucumis melo])

HSP 1 Score: 276.9 bits (707), Expect = 1.2e-70
Identity = 149/213 (69.95%), Postives = 159/213 (74.65%), Query Frame = 0


Query: 1   MMLLLKKSIGKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYS 60
           M+ LLKK IGKTTGGSVPVYLNVYDLTAINGYAYW GLGVFHSGVQVHGVEYAFGAHEYS
Sbjct: 1   MVFLLKKPIGKTTGGSVPVYLNVYDLTAINGYAYWLGLGVFHSGVQVHGVEYAFGAHEYS 60

Query: 61  TTGIFEGVPKQCDGFRFRKTVLVGKTDMKPTE---------------------------- 120
           TTGIFEG+PKQCDGFRFRKT+LVGKTDMKPTE                            
Sbjct: 61  TTGIFEGMPKQCDGFRFRKTILVGKTDMKPTEVRALMEELAQIYKGNAYNLITKNCNHFC 120

Query: 121 ----------GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKE 176
                      IP+ +  +A    + FLCNCVLPVT NST+IRHH RIEDKVVTMET KE
Sbjct: 121 NDACIKLTGNSIPNWVNRLA---RIGFLCNCVLPVTLNSTRIRHHQRIEDKVVTMETNKE 180

[Close]

BLAST of Lsi02G026730 vs. NCBI nr
Match: XP_011659506.1 (deSI-like protein At4g17486 [Cucumis sativus] >KGN45226.1 hypothetical protein Csa_016563 [Cucumis sativus])

HSP 1 Score: 276.2 bits (705), Expect = 2.0e-70
Identity = 150/213 (70.42%), Postives = 158/213 (74.18%), Query Frame = 0


Query: 1   MMLLLKKSIGKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYS 60
           M+ LLKK IGKTTGGSVPVYLNVYDLTAINGYAYW GLGVFHSGVQVHGVEYAFGAHEYS
Sbjct: 1   MVFLLKKPIGKTTGGSVPVYLNVYDLTAINGYAYWLGLGVFHSGVQVHGVEYAFGAHEYS 60

Query: 61  TTGIFEGVPKQCDGFRFRKTVLVGKTDMKPTE---------------------------- 120
           TTGIFEGVPKQCDGFRFRKT+LVGKTDMKPTE                            
Sbjct: 61  TTGIFEGVPKQCDGFRFRKTILVGKTDMKPTEVRSLMEELAQIYKGNAYNLITKNCNHFC 120

Query: 121 ----------GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKE 176
                      IP+ +  +A    + FLCNCVLPVT NST+IRHHHRIEDKVVTME KKE
Sbjct: 121 NDACIKLTGNSIPNWVNRLA---RIGFLCNCVLPVTLNSTRIRHHHRIEDKVVTMEAKKE 180

[Close]

BLAST of Lsi02G026730 vs. NCBI nr
Match: XP_022959764.1 (deSI-like protein At4g17486 [Cucurbita moschata])

HSP 1 Score: 273.1 bits (697), Expect = 1.7e-69
Identity = 146/212 (68.87%), Postives = 158/212 (74.53%), Query Frame = 0


Query: 2   MLLLKKSIGKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYST 61
           MLL KK +GK TGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYST
Sbjct: 1   MLLFKKPVGKQTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYST 60

Query: 62  TGIFEGVPKQCDGFRFRKTVLVGKTDMKPTE----------------------------- 121
           TGIFEGVPKQC GFRFRK+VL+GKTDMKP+E                             
Sbjct: 61  TGIFEGVPKQCHGFRFRKSVLIGKTDMKPSEVRALMEELAQIYKGNAYNLITKNCNHFCN 120

Query: 122 ---------GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKEL 176
                     IP+ +  +A    + FLCNCVLPVTFNST+IRHHHRIEDKVVTMETKK+L
Sbjct: 121 DACVKLTENSIPNWVNRLA---RIGFLCNCVLPVTFNSTRIRHHHRIEDKVVTMETKKDL 180

[Close]

BLAST of Lsi02G026730 vs. TAIR 10
Match: AT1G80690.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 162.9 bits (411), Expect = 2.3e-40
Identity = 95/212 (44.81%), Postives = 126/212 (59.43%), Query Frame = 0


Query: 3   LLLKKSIGKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYSTT 62
           +L +K+      G+VPVYLNVYDLT INGYAYW GLGV+HSGV+VHG+EYA+GAHEY +T
Sbjct: 1   MLCRKNSSLVDRGNVPVYLNVYDLTPINGYAYWLGLGVYHSGVEVHGIEYAYGAHEYPST 60

Query: 63  GIFEGVPKQCDGFRFRKTVLVGKTDMKPTE------------------------------ 122
           GIFEG PKQC+GF FRK++L+GKTD+ P E                              
Sbjct: 61  GIFEGEPKQCEGFTFRKSILIGKTDLGPLEVRATMEQLADNYKGSSYNLITKNCNHFCDE 120

Query: 123 --------GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMET-KKEL 176
                    IPS +  +A    + F+CNCVLP T N+T+  ++   +DK    E  KK+L
Sbjct: 121 TCIKLTGNPIPSWVNRLA---RIGFMCNCVLPATINATRFGNNRVNQDKSCEAENEKKKL 180

[Close]

BLAST of Lsi02G026730 vs. TAIR 10
Match: AT5G25170.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 150.6 bits (379), Expect = 1.2e-36
Identity = 87/189 (46.03%), Postives = 107/189 (56.61%), Query Frame = 0


Query: 11  KTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYSTTGIFEGVPK 70
           K   GSVPVYLNVYDLT INGYAYW GLG++HSGV+VHGVEY FGAH++STTGIFE  PK
Sbjct: 12  KKKPGSVPVYLNVYDLTPINGYAYWLGLGIYHSGVEVHGVEYGFGAHDHSTTGIFEVEPK 71

Query: 71  QCDGFRFRKTVLVGKTDMKP--------------------------------------TE 130
           QC GF FRK++L+G+TD+ P                                        
Sbjct: 72  QCPGFTFRKSILIGRTDLDPENVRVFMEKLAEEYSGNSYHLITKNCNHFCNDVCVQLTRR 131

Query: 131 GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKELTSESTKTPS 162
            IPS +  +A   L    CNCVLP   N TK+R     E+K+  +E KK+L S S++ P 
Sbjct: 132 SIPSWVNRLARFGL---FCNCVLPAELNETKVRQVRSKEEKIPEVE-KKKLRSRSSRFPP 191

[Close]

BLAST of Lsi02G026730 vs. TAIR 10
Match: AT2G25190.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 137.5 bits (345), Expect = 1.0e-32
Identity = 87/199 (43.72%), Postives = 107/199 (53.77%), Query Frame = 0


Query: 2   MLLLKKSI-GKTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYS 61
           ML  K S+  K   GSVPVYLNVYDLT +N Y YW GLGVFHSGV+VHGVEYAFGAHE S
Sbjct: 1   MLCFKGSVKRKKQSGSVPVYLNVYDLTPMNAYGYWLGLGVFHSGVEVHGVEYAFGAHESS 60

Query: 62  TTGIFEGVPKQCDGFRFRKTVLVGKTDMKPTE---------------------------- 121
           +TGIFE  PK+C GF FRK++LVGKTD+   E                            
Sbjct: 61  STGIFEVEPKKCPGFTFRKSILVGKTDLVAKEVRVFMEKLAEEYQGNKYHLITRNCNHFC 120

Query: 122 ----------GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHRIEDKVVTMETKKE 162
                      IP  +  +A L     LCNCVLP   N  K+R   R+    ++   KK+
Sbjct: 121 NEVCLKLAQKSIPRWVNRLARL---GVLCNCVLPPRLNEAKVR---RVGKGELSESEKKK 180

[Close]

BLAST of Lsi02G026730 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 129.8 bits (325), Expect = 2.1e-30
Identity = 80/205 (39.02%), Postives = 107/205 (52.20%), Query Frame = 0


Query: 11  KTTGGSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYSTTGIFEGVPK 70
           K   G+VPVYLNVYDLT +N Y YW G+G++HSG++VHGVEY +GAHE S++GIFE  PK
Sbjct: 11  KKNSGTVPVYLNVYDLTPMNVYGYWLGIGIYHSGLEVHGVEYGYGAHEKSSSGIFEVEPK 70

Query: 71  QCDGFRFRKTVLVGKTDMKPTE-------------------------------------- 130
           +C GF FRK++LVG+T+MK  E                                      
Sbjct: 71  KCPGFTFRKSILVGETEMKAKEVRSFMEKLSEEYQGNKYHLITRNCNHFCNHVSLKLTHK 130

Query: 131 GIPSQIGLIALLELVSFLCNCVLPVTFNSTKIRHHHR-----IEDKVVTMETKKELTSES 173
            IPS +  +A L    FLCNCVLP   N TK++   +     +E +    + +K+    S
Sbjct: 131 SIPSWVNRLARL---GFLCNCVLPACLNETKVKRVGKDGKLLLEGENTKKKKRKKKIRRS 190

[Close]

BLAST of Lsi02G026730 vs. TAIR 10
Match: AT1G47740.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 128.3 bits (321), Expect = 6.2e-30
Identity = 52/78 (66.67%), Postives = 67/78 (85.90%), Query Frame = 0


Query: 15  GSVPVYLNVYDLTAINGYAYWFGLGVFHSGVQVHGVEYAFGAHEYSTTGIFEGVPKQCDG 74
           G  PVYLNVYDLT INGY YW GLG+FHSGV+VHGVEYAFGAH+Y+T+G+FE  P+QC G
Sbjct: 67  GRAPVYLNVYDLTPINGYIYWAGLGIFHSGVEVHGVEYAFGAHDYATSGVFEVEPRQCPG 126

Query: 75  FRFRKTVLVGKTDMKPTE 93
           F+F+K++ +G T++ PT+
Sbjct: 127 FKFKKSIFIGTTNLNPTQ 144

The following BLAST results are available for this feature:

BLAST of Lsi02G026730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VG8	2.5e-23	57.33	DeSI-like protein At4g17486 OS=Arabidopsis thaliana OX=3702 GN=At4g17486 PE=2 SV...	[more]
H2KZK4	4.0e-13	42.31	Desumoylating isopeptidase 1 homolog OS=Caenorhabditis elegans OX=6239 GN=F36D4....	[more]
Q5PQ09	3.3e-12	45.95	Deubiquitinase DESI2 OS=Xenopus laevis OX=8355 GN=desi2 PE=2 SV=1	[more]
Q6DC39	5.7e-12	39.77	Deubiquitinase DESI2 OS=Danio rerio OX=7955 GN=desi2 PE=2 SV=1	[more]
Q9BSY9	2.8e-11	43.59	Deubiquitinase DESI2 OS=Homo sapiens OX=9606 GN=DESI2 PE=1 SV=1	[more]

BLAST of Lsi02G026730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UYX3	6.7e-72	70.89	DeSI-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00...	[more]
A0A1S3CEJ5	5.6e-71	69.95	deSI-like protein At4g17486 OS=Cucumis melo OX=3656 GN=LOC103499833 PE=3 SV=1	[more]
A0A0A0KBP9	9.6e-71	70.42	PPPDE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G432070 PE=3 ...	[more]
A0A6J1H916	8.1e-70	68.87	deSI-like protein At4g17486 OS=Cucurbita moschata OX=3662 GN=LOC111460737 PE=3 S...	[more]
A0A6J1KRJ3	3.4e-68	67.92	deSI-like protein At4g17486 OS=Cucurbita maxima OX=3661 GN=LOC111498027 PE=3 SV=...	[more]

BLAST of Lsi02G026730 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0058719.1	1.4e-71	70.89	deSI-like protein [Cucumis melo var. makuwa] >TYK10526.1 deSI-like protein [Cucu...	[more]
XP_038899104.1	8.9e-71	71.36	deSI-like protein At4g17486 [Benincasa hispida]	[more]
XP_008461171.1	1.2e-70	69.95	PREDICTED: deSI-like protein At4g17486 [Cucumis melo]	[more]
XP_011659506.1	2.0e-70	70.42	deSI-like protein At4g17486 [Cucumis sativus] >KGN45226.1 hypothetical protein C...	[more]
XP_022959764.1	1.7e-69	68.87	deSI-like protein At4g17486 [Cucurbita moschata]	[more]

BLAST of Lsi02G026730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80690.1	2.3e-40	44.81	PPPDE putative thiol peptidase family protein	[more]
AT5G25170.1	1.2e-36	46.03	PPPDE putative thiol peptidase family protein	[more]
AT2G25190.1	1.0e-32	43.72	PPPDE putative thiol peptidase family protein	[more]
AT4G31980.1	2.1e-30	39.02	unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,...	[more]
AT1G47740.1	6.2e-30	66.67	PPPDE putative thiol peptidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008580	PPPDE peptidase domain	SMART	SM01179	DUF862_2a	coord: 17..139 e-value: 9.4E-12 score: 55.0
IPR008580	PPPDE peptidase domain	PFAM	PF05903	Peptidase_C97	coord: 18..91 e-value: 8.9E-19 score: 67.8
IPR008580	PPPDE peptidase domain	PANTHER	PTHR12378	DESUMOYLATING ISOPEPTIDASE	coord: 5..92 coord: 101..172
IPR008580	PPPDE peptidase domain	PROSITE	PS51858	PPPDE	coord: 17..143 score: 26.904873
IPR042266	PPPDE peptidase domain superfamily	GENE3D	3.90.1720.30	PPPDE domains	coord: 13..95 e-value: 8.4E-17 score: 63.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 139..175
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 146..169
None	No IPR available	PANTHER	PTHR12378:SF52	F23A5.4 PROTEIN	coord: 5..92 coord: 101..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G026730.1	Lsi02G026730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016579	protein deubiquitination
molecular_function	GO:0101005	deubiquitinase activity
molecular_function	GO:0008233	peptidase activity