Lsi02G026480 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G026480
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptiontubby-like protein 8
Locationchr02: 32900210 .. 32903475 (-)
RNA-Seq ExpressionLsi02G026480
SyntenyLsi02G026480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGAGACTAGACCACAAAATACCATCCCCATTCTTCCATTTTCTTAACTCTTCCCCTGTTCTTGTCTCAACACTTCAACAAACAAAAGGATGATTGGCGACAAGAAAGCGATTATACCTCGCCAATCTTCCTATAATTCTTTGTATTTGAATCCCCTTACTGACCTCCGGCACAACCGTAGCTGCAGCGACGGCGGCGGATTCCGGCAAGGTAAAGAAAACTTGATCCCTAGTACGAAGGATAAACAGGGAGATGAAAATGCAGTGTTTTGCGACAAAGAAAATGCGATTCCCATTAATGGGTTTTGTGCTAATTCGAAGCAGCAATTTGGGGATTTTAAGTCATTTTCGACTGGAAAGGCTTTGAAGCCTTCGTCACTTCGTCTGTGTATGCAGATGAACGAACCCGATAAGGTTTTTGCCTCTAGATTGTGGGATGCCACTGATTCCGAACATTCAAGTTCTTTGAAGATTTGGGACTTTTCGGATTCGGAAGCTGCTCCAGCTTCCTCATGGTCCACTTTGCCAAACAGGTGGATTTTAGAACTTCAACACCGTTGATAGACTTGTTGATGATAAATTTTGGCCTCAGTTTCATAACCATTTGAGTTTCAGCTTTTGATAATAGAGCAGAACACTACTTTTTACTTATGAGTTTATGAGTTTCTTTGTTATGTTATCAATTATTTTAGGTGTTTTCAAAATCAAAACAAGTTTTGAAAACATTCTCTTTGATAACCATTTGATTTTTAGTTTTTGAAAATTGAGCTTATAAATACTAGTTTTTTACTTATGAGTTTCTTTGTTATGTTATCTACCTTTTAGGTGTTTCCAAAATCAAAACAAGTTTTGAAAACGTTAGGTTTGATGACCATTTGATTTTAGTTTTTGAAAATTAAGCTTATAAACTCTATTTCTGCTCATTGATTTCTTTGTTTTGCTACCTATATTTTAAGAGTGGTTTAAAAAATCAAGCCAAAATTTCTTTAAAAAGATGAAAACCATTGTAAAGAAATGATGAGAATACAAGCAGAATTTTAACAAACCAAATGGTTATCCAAATGTGCCTAAGTTTTAAGTTTTTTGGTTTAGTAGAAGGGGGACATTGTTTCCATCCATGTCCTCTTTTCTCTGATTAATGTTATTTCGAGCTTTTTGAGGATGTTGGGAATGATTGTGTAAATGGTATTTTTTCTTTTCAGGTCATTGTTGTGTAGGCCATTGCCTGTGGATGTGGGAAGATGTACTTGTGTCATTGTGAAGGAAAAATCACCAGATGGGCTTCATGGTGGAGCCTTCTATTCTCTATACACCAATGTAAGTTTGTGGCTTTCAGATTCTTTTTAATAACAAAACATATAACTTATCAACTGGGGTTCTGTTCTTAATGATTCACGTAGCTTGCTTATGTTAAATCTTAACTGATCAGCTTTAGATTTTTTAGGGATTGATGTATTTACATTTCTCTGACCTTCATATTTTGATTAACTTTCTTTAAATCAAGTGTGTCTATTGAGAAATTTAGGCCTTTTCAATTCAACTCTTCAAGAAAAATAGAACACAAGAAACTCCCAAATCCTGCAACTTAGTAGAGGATTCCCTTGTTAATCTTCAATCTATGTTTTTTGCATTTGAAAATTTATCAGGAAGGGAAGGGACGGCAGGACCGGAAGTTGGCGGTGGCTCGCCATCGACGACGGAACGGGAGGTCAGAGTATAAAATAGCTCAGAGTGCAAGGGGGATATTGTGCAATGCAGATGATAGTTTTGTTGGATCCATGACTACAAACCTTGTTGGTTCAAAATATCATATATGGGATCAGGTTCTAATGGAATGTTAAATTGTGTTAAACTTCAAAGAAGTTTGCAGAGTTTCTATGATTGTGACATGGTTTTCTCTTCATGTTTGTTCTCAGGGGAGCCGTCTGAAACCAATCCCAATCCAGTCGAAATCGCTTTTGGCTGTTGTAACGTAAGCCACGGTGTTATTTATCTCTTTTCTCCCTCTCTATCTGTCTTTTAGAGTCATTGATTTGGCTTTGTTTTCTACAGATTCATGCCTACAATAACCACTTGGACAGGCAGCCATAGGAGCATGAAGGCTTTCATCCCCAAGCATCAATCAATGCAGCTAAAGAACACCAATCAGGTGCTTTAGAACATTTGTGATATTCTTTCTAATTTATACATGTGAAAGTTCGCACCACCCGCAATGCTTTTTGATACTTCCACATTTGATGAAAATGTTGATAGAAACATCGACAAAACATTGACAAAACATCAATGCTGATAGATAATTTGAGAAAAAGTCGTCAAGTACATAAGTGCATCGAAGTAGTTTGATGTTAAATCTCAATATATCTCGACATTTGTCTTACTTTTTCCGAACCCTTTAATTTTACTGCTTATTTACTTCAGCAGGTGCAGCACATAGCAGGGCTGCCAAGGGAGTGGGAGGAAGAAAAGGCCAAAGTCCACCAGCTTGTTTCTAAGGTTCCCCAATACAACAAGGTGAATTAGAAAAATTGTCATTCAACTTTTCAATGAAATCTTGGAATTGCTTTGGATTCCTCATTGTTTTATTGCTTTTGACTTCTTCAGATTTCTAAGCAATATGAGCTGGACTTTAGAGACAGAGGAAGGGCAGTTTCAGGTCTTATGATCCAAACCTCAGTGAAGAATTTTCAGTTAACTTTAGAGGTACAAATCCAAATTTTTTAGAGTACTCACTTTGGGATCAAATAGGATAGAAAGAAAGTGATTTTCAATTATAAGAAGAGGCTGAGTTTTTTTGAGTCGGTTTTTTTGGCACAGGAAAATGGCAAACAAACAATTCTCCAACTTGGAAGGATTGGGAAGTCTAAGTATGTGATGGATTATAGGTAAGCGGATCATGTTTTGTATTCTCTTGAAATTTAATCCGACCAAACCAACCAAACCGAGCAAAAATTAGCTAAAAATCATATCAAAGTTCAACAAAATCGTAATACATCGACCGAAACCGAACAAAACTGAATGTATTTTGGTTTGAAACTGTAGTGATTGTATTTGGCTACTGGTTTTCCCTTTCAATTTTATAATTAGGATCTTGATGTGAATTATGATAGAGTGAGAGTTTTGGCCTTCTTGCAGATATCCATTGACAGGTTATCAAGCATTTTGCATATGCCTGGCTTCCATAGATTCGAAGCTGTGTTGCTCAGTATGATCATATTTGGCTGCTTTTCAGCCCTCTTCTGCAGTTCAATCTCTGAACTAGCTTCCTGA

mRNA sequence

TAGAGACTAGACCACAAAATACCATCCCCATTCTTCCATTTTCTTAACTCTTCCCCTGTTCTTGTCTCAACACTTCAACAAACAAAAGGATGATTGGCGACAAGAAAGCGATTATACCTCGCCAATCTTCCTATAATTCTTTGTATTTGAATCCCCTTACTGACCTCCGGCACAACCGTAGCTGCAGCGACGGCGGCGGATTCCGGCAAGGTAAAGAAAACTTGATCCCTAGTACGAAGGATAAACAGGGAGATGAAAATGCAGTGTTTTGCGACAAAGAAAATGCGATTCCCATTAATGGGTTTTGTGCTAATTCGAAGCAGCAATTTGGGGATTTTAAGTCATTTTCGACTGGAAAGGCTTTGAAGCCTTCGTCACTTCGTCTGTGTATGCAGATGAACGAACCCGATAAGGTTTTTGCCTCTAGATTGTGGGATGCCACTGATTCCGAACATTCAAGTTCTTTGAAGATTTGGGACTTTTCGGATTCGGAAGCTGCTCCAGCTTCCTCATGGTCCACTTTGCCAAACAGGTCATTGTTGTGTAGGCCATTGCCTGTGGATGTGGGAAGATGTACTTGTGTCATTGTGAAGGAAAAATCACCAGATGGGCTTCATGGTGGAGCCTTCTATTCTCTATACACCAATGAAGGGAAGGGACGGCAGGACCGGAAGTTGGCGGTGGCTCGCCATCGACGACGGAACGGGAGGTCAGAGTATAAAATAGCTCAGAGTGCAAGGGGGATATTGTGCAATGCAGATGATAGTTTTGTTGGATCCATGACTACAAACCTTGTTGGTTCAAAATATCATATATGGGATCAGGGGAGCCGTCTGAAACCAATCCCAATCCAGTCGAAATCGCTTTTGGCTGTTGTAACATTCATGCCTACAATAACCACTTGGACAGGCAGCCATAGGAGCATGAAGGCTTTCATCCCCAAGCATCAATCAATGCAGCTAAAGAACACCAATCAGGTGCAGCACATAGCAGGGCTGCCAAGGGAGTGGGAGGAAGAAAAGGCCAAAGTCCACCAGCTTGTTTCTAAGGTTCCCCAATACAACAAGATTTCTAAGCAATATGAGCTGGACTTTAGAGACAGAGGAAGGGCAGTTTCAGGTCTTATGATCCAAACCTCAGTGAAGAATTTTCAGTTAACTTTAGAGGAAAATGGCAAACAAACAATTCTCCAACTTGGAAGGATTGGGAAGTCTAAGTATGTGATGGATTATAGAGTGAGAGTTTTGGCCTTCTTGCAGATATCCATTGACAGGTTATCAAGCATTTTGCATATGCCTGGCTTCCATAGATTCGAAGCTGTGTTGCTCAGTATGATCATATTTGGCTGCTTTTCAGCCCTCTTCTGCAGTTCAATCTCTGAACTAGCTTCCTGA

Coding sequence (CDS)

ATGATTGGCGACAAGAAAGCGATTATACCTCGCCAATCTTCCTATAATTCTTTGTATTTGAATCCCCTTACTGACCTCCGGCACAACCGTAGCTGCAGCGACGGCGGCGGATTCCGGCAAGGTAAAGAAAACTTGATCCCTAGTACGAAGGATAAACAGGGAGATGAAAATGCAGTGTTTTGCGACAAAGAAAATGCGATTCCCATTAATGGGTTTTGTGCTAATTCGAAGCAGCAATTTGGGGATTTTAAGTCATTTTCGACTGGAAAGGCTTTGAAGCCTTCGTCACTTCGTCTGTGTATGCAGATGAACGAACCCGATAAGGTTTTTGCCTCTAGATTGTGGGATGCCACTGATTCCGAACATTCAAGTTCTTTGAAGATTTGGGACTTTTCGGATTCGGAAGCTGCTCCAGCTTCCTCATGGTCCACTTTGCCAAACAGGTCATTGTTGTGTAGGCCATTGCCTGTGGATGTGGGAAGATGTACTTGTGTCATTGTGAAGGAAAAATCACCAGATGGGCTTCATGGTGGAGCCTTCTATTCTCTATACACCAATGAAGGGAAGGGACGGCAGGACCGGAAGTTGGCGGTGGCTCGCCATCGACGACGGAACGGGAGGTCAGAGTATAAAATAGCTCAGAGTGCAAGGGGGATATTGTGCAATGCAGATGATAGTTTTGTTGGATCCATGACTACAAACCTTGTTGGTTCAAAATATCATATATGGGATCAGGGGAGCCGTCTGAAACCAATCCCAATCCAGTCGAAATCGCTTTTGGCTGTTGTAACATTCATGCCTACAATAACCACTTGGACAGGCAGCCATAGGAGCATGAAGGCTTTCATCCCCAAGCATCAATCAATGCAGCTAAAGAACACCAATCAGGTGCAGCACATAGCAGGGCTGCCAAGGGAGTGGGAGGAAGAAAAGGCCAAAGTCCACCAGCTTGTTTCTAAGGTTCCCCAATACAACAAGATTTCTAAGCAATATGAGCTGGACTTTAGAGACAGAGGAAGGGCAGTTTCAGGTCTTATGATCCAAACCTCAGTGAAGAATTTTCAGTTAACTTTAGAGGAAAATGGCAAACAAACAATTCTCCAACTTGGAAGGATTGGGAAGTCTAAGTATGTGATGGATTATAGAGTGAGAGTTTTGGCCTTCTTGCAGATATCCATTGACAGGTTATCAAGCATTTTGCATATGCCTGGCTTCCATAGATTCGAAGCTGTGTTGCTCAGTATGATCATATTTGGCTGCTTTTCAGCCCTCTTCTGCAGTTCAATCTCTGAACTAGCTTCCTGA

Protein sequence

MIGDKKAIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLIPSTKDKQGDENAVFCDKENAIPINGFCANSKQQFGDFKSFSTGKALKPSSLRLCMQMNEPDKVFASRLWDATDSEHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAFYSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGSKYHIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHIAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEENGKQTILQLGRIGKSKYVMDYRVRVLAFLQISIDRLSSILHMPGFHRFEAVLLSMIIFGCFSALFCSSISELAS
Homology
BLAST of Lsi02G026480 vs. ExPASy Swiss-Prot
Match: Q9S9M8 (Tubby-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=TULP8 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 4.0e-107
Identity = 193/305 (63.28%), Postives = 245/305 (80.33%), Query Frame = 0

Query: 82  DFKSFSTG--KALKPSSLRLCMQMN-EPDK-VFASRLWDATDSEHSSSLKIWDFSDSEAA 141
           +FKSFSTG   ALK SSL+ CMQ N E DK  F  + W + DSEHSSSLK+W+FSDSEAA
Sbjct: 71  NFKSFSTGGRTALKQSSLQACMQKNSEVDKSSFGMKTWTSVDSEHSSSLKVWEFSDSEAA 130

Query: 142 PASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAFYSLYTNEGKGRQDRKLA 201
           PASSWSTLPNR+LLC+ LP+DVGRCTC+IVKE+SP+GL  G+ YSLYT+EG+GR+DRKLA
Sbjct: 131 PASSWSTLPNRALLCKTLPLDVGRCTCLIVKEQSPEGLSHGSVYSLYTHEGRGRKDRKLA 190

Query: 202 VARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGSKYHIWDQGSRLKPIPIQSK 261
           VA H RRNG+S +++AQ+ +G+LC++D+S+VGSMT NL+GSKY+IWD+G R+  +    K
Sbjct: 191 VAYHSRRNGKSIFRVAQNVKGLLCSSDESYVGSMTANLLGSKYYIWDKGVRVGSVGKMVK 250

Query: 262 SLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHIAGLPREWEEEKAKVHQL 321
            LL+VV F PTITTWTGS+R M+  +PK Q MQ  N  QVQ  + LP +W E K K+ +L
Sbjct: 251 PLLSVVIFTPTITTWTGSYRRMRTLLPKQQPMQKNNNKQVQQASKLPLDWLENKEKIQKL 310

Query: 322 VSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEENGKQTILQLGRIGKSKY 381
            S++P YNKISKQ+ELDFRDRGR  +GL IQ+SVKNFQLTL E  +QTILQ+GR+ K++Y
Sbjct: 311 CSRIPHYNKISKQHELDFRDRGR--TGLRIQSSVKNFQLTLTETPRQTILQMGRVDKARY 370

Query: 382 VMDYR 383
           V+D+R
Sbjct: 371 VIDFR 373

BLAST of Lsi02G026480 vs. ExPASy Swiss-Prot
Match: Q6ETL2 (Tubby-like protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=TULP4 PE=3 SV=2)

HSP 1 Score: 345.9 bits (886), Expect = 6.6e-94
Identity = 171/276 (61.96%), Postives = 218/276 (78.99%), Query Frame = 0

Query: 123 SSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKE---KSPDGLHGGA 182
           SSS + WD SD+EAAPASSW+TLPNR+LLCRPLP+DVGRCTC+I KE    +  G  G A
Sbjct: 132 SSSYEAWDLSDNEAAPASSWATLPNRALLCRPLPLDVGRCTCIIAKETLAAAAAGARGVA 191

Query: 183 FYSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGSK 242
            YSLYTNEG+GRQDRKLAVARHRRR GRSE+ +AQ+  GI C +D +F+G++++NLVGS+
Sbjct: 192 LYSLYTNEGQGRQDRKLAVARHRRRRGRSEFVVAQNLDGIFCTSDKNFLGTLSSNLVGSR 251

Query: 243 YHIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTN--QV 302
           Y IW QG+R+  I  QSK LL VV F PT+TT TGS RSM+A+IPK+QS+ LKN+N  Q+
Sbjct: 252 YRIWGQGNRVDEIKSQSKRLLGVVAFAPTVTTLTGSFRSMRAWIPKNQSIHLKNSNSAQI 311

Query: 303 QHIAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLT 362
           QHI+GLP++W+E+K K  QL S+ P YN ++K+YELDFR+R   + G  +Q SVKNFQ+T
Sbjct: 312 QHISGLPKDWQEKKIKADQLCSRSPFYNNMTKRYELDFRERAGRM-GYKVQPSVKNFQMT 371

Query: 363 LEENGKQTILQLGRIGKSKYVMDYRVRVLAFLQISI 394
           LEE G+QTILQLGRIGKSKY+MD+R  +  +  + I
Sbjct: 372 LEEKGRQTILQLGRIGKSKYIMDFRYPLTGYQALCI 406

BLAST of Lsi02G026480 vs. ExPASy Swiss-Prot
Match: B0XFQ9 (Protein king tubby 1 OS=Culex quinquefasciatus OX=7176 GN=king-tubby1 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 3.7e-12
Identity = 73/290 (25.17%), Postives = 124/290 (42.76%), Query Frame = 0

Query: 119 DSEHSSSLKIWDFSDSEAA-----------PASSWSTLP-NRSLLCRPLPVDVGRCTCVI 178
           DS  S +LK  + S  +A+           P   W T P  + +L +          C I
Sbjct: 148 DSGSSGNLKSMEHSSPQASGHNDAEGDVAGPIEQWVTQPAAQGVLYK----------CRI 207

Query: 179 VKEKSPDGLHGGAFYSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDS 238
            +++   G+  G F   Y +  +    R   +A  +R+  ++   I       L    D 
Sbjct: 208 TRDRK--GMDRGLFPIYYLHLERDYGKRLFCLAGRKRKKSKTSNYIISCDPTDLSRQADG 267

Query: 239 FVGSMTTNLVGSKYHIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIP-- 298
           FVG + +N+ G+ + ++D G   K  P+  +  LAVV +   I  + G  R+M   +P  
Sbjct: 268 FVGKLRSNVFGTTFFVYDNGK--KDDPVSPRLDLAVVIYDTNILGFKGP-RNMTVLLPGM 327

Query: 299 KHQSMQLKNTNQVQHIAGLPREWEEEKA-KVHQLVSKVPQYNKISKQYELDFRDRGRAVS 358
                ++K  +   H  GL   W+ +    V +L +K P +N  ++ Y L+F        
Sbjct: 328 TEDDQRVKINSSDSHGQGLLDSWKSKHMDNVVELHNKTPIWNDETQSYVLNFH------- 387

Query: 359 GLMIQTSVKNFQLTLEENGKQTILQLGRIGKSKYVMDYRVRVLAFLQISI 394
           G + Q SVKNFQL  + +    ++Q GR     + MD+R  + AF   +I
Sbjct: 388 GRVTQASVKNFQLVHDSDPDYIVMQFGRTSDDIFTMDFRYPLCAFQAFAI 415

BLAST of Lsi02G026480 vs. ExPASy Swiss-Prot
Match: B0WI30 (Protein king tubby 2 OS=Culex quinquefasciatus OX=7176 GN=king-tubby2 PE=3 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 3.7e-12
Identity = 73/290 (25.17%), Postives = 124/290 (42.76%), Query Frame = 0

Query: 119 DSEHSSSLKIWDFSDSEAA-----------PASSWSTLP-NRSLLCRPLPVDVGRCTCVI 178
           DS  S +LK  + S  +A+           P   W T P  + +L +          C I
Sbjct: 143 DSGSSGNLKSMEHSSPQASGHNDAEGDVAGPIEQWVTQPAAQGVLYK----------CRI 202

Query: 179 VKEKSPDGLHGGAFYSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDS 238
            +++   G+  G F   Y +  +    R   +A  +R+  ++   I       L    D 
Sbjct: 203 TRDRK--GMDRGLFPIYYLHLERDYGKRLFCLAGRKRKKSKTSNYIISCDPTDLSRQADG 262

Query: 239 FVGSMTTNLVGSKYHIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIP-- 298
           FVG + +N+ G+ + ++D G   K  P+  +  LAVV +   I  + G  R+M   +P  
Sbjct: 263 FVGKLRSNVFGTTFFVYDNGK--KDDPVSPRLDLAVVIYDTNILGFKGP-RNMTVLLPGM 322

Query: 299 KHQSMQLKNTNQVQHIAGLPREWEEEKA-KVHQLVSKVPQYNKISKQYELDFRDRGRAVS 358
                ++K  +   H  GL   W+ +    V +L +K P +N  ++ Y L+F        
Sbjct: 323 TEDDQRVKINSSDSHGQGLLDSWKSKHMDNVVELHNKTPIWNDETQSYVLNFH------- 382

Query: 359 GLMIQTSVKNFQLTLEENGKQTILQLGRIGKSKYVMDYRVRVLAFLQISI 394
           G + Q SVKNFQL  + +    ++Q GR     + MD+R  + AF   +I
Sbjct: 383 GRVTQASVKNFQLVHDSDPDYIVMQFGRTSDDIFTMDFRYPLCAFQAFAI 410

BLAST of Lsi02G026480 vs. ExPASy Swiss-Prot
Match: P50607 (Tubby protein homolog OS=Homo sapiens OX=9606 GN=TUB PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 3.2e-11
Identity = 65/253 (25.69%), Postives = 118/253 (46.64%), Query Frame = 0

Query: 153 RPLPVDVGRCTCVIVKEKSPDGLHGGAF--YSLYTNEGKGRQDRKLAVARHRRRNGRSEY 212
           RP P  +    C I ++K   G+  G +  Y L+ +   G++   LA  R R+++  S Y
Sbjct: 257 RPAPQGI-TIKCRITRDKK--GMDRGMYPTYFLHLDREDGKKVFLLA-GRKRKKSKTSNY 316

Query: 213 KIAQSARGILCNADDSFVGSMTTNLVGSKYHIWDQG---SRLKPIPIQSKSL---LAVVT 272
            I+      L    DS++G + +NL+G+K+ ++D G    +     ++S +L   LA V 
Sbjct: 317 LISVDPTD-LSRGGDSYIGKLRSNLMGTKFTVYDNGVNPQKASSSTLESGTLRQELAAVC 376

Query: 273 FMPTITTWTGSHRSMKAFIP----KHQSMQLKNTNQVQHIAGLPREWEEEKAKVHQLVSK 332
           +   +  + G  R M   +P     H+ + ++  N  +H   L R   +    + +L +K
Sbjct: 377 YETNVLGFKGP-RKMSVIVPGMNMVHERVSIRPRN--EHETLLARWQNKNTESIIELQNK 436

Query: 333 VPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEENGKQTILQLGRIGKSKYVMD 392
            P +N  ++ Y L+F        G + Q SVKNFQ+    +    ++Q GR+ +  + MD
Sbjct: 437 TPVWNDDTQSYVLNFH-------GRVTQASVKNFQIIHGNDPDYIVMQFGRVAEDVFTMD 494

Query: 393 YRVRVLAFLQISI 394
           Y   + A    +I
Sbjct: 497 YNYPLCALQAFAI 494

BLAST of Lsi02G026480 vs. ExPASy TrEMBL
Match: A0A5D3CJY1 (Tubby-like protein 8 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001490 PE=3 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 3.5e-207
Identity = 364/400 (91.00%), Postives = 372/400 (93.00%), Query Frame = 0

Query: 1   MIGDKKAIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLIPSTKDKQGDENAVF 60
           MIGDKK+IIPRQSSYNSLYLNPLTDL HNRSCSDGGGF+QGKENL P+TKDKQ DE+AV 
Sbjct: 1   MIGDKKSIIPRQSSYNSLYLNPLTDLSHNRSCSDGGGFKQGKENLTPNTKDKQRDESAVL 60

Query: 61  CDKENAIPINGFCANSKQQFGDFKSFSTGKALKPSSLRLCMQMNEPDKVFASRLWDATDS 120
           CDKENAIPINGFCANSKQQFGD KSFSTGKALKPSSL LCMQMNEPDKVFA+RLWD TDS
Sbjct: 61  CDKENAIPINGFCANSKQQFGDLKSFSTGKALKPSSLHLCMQMNEPDKVFATRLWDGTDS 120

Query: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180
           EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF
Sbjct: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180

Query: 181 YSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGSKY 240
           YSLYTNEGKGRQDRKLAVARHRRR GRSEYKIAQS RG+LCNADDSFVGSMTTNLVGSKY
Sbjct: 181 YSLYTNEGKGRQDRKLAVARHRRRKGRSEYKIAQSTRGVLCNADDSFVGSMTTNLVGSKY 240

Query: 241 HIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHI 300
           HIWDQG+RLKP     +SLL  VTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHI
Sbjct: 241 HIWDQGNRLKP-----RSLLGAVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHI 300

Query: 301 AGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEE 360
           AGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEE
Sbjct: 301 AGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEE 360

Query: 361 NGKQTILQLGRIGKSKYVMDY-----------RVRVLAFL 390
           NGKQTILQLGRIGKSKYVMDY           RVRVL FL
Sbjct: 361 NGKQTILQLGRIGKSKYVMDYSANRDVDVNNDRVRVLGFL 395

BLAST of Lsi02G026480 vs. ExPASy TrEMBL
Match: A0A0A0KBM7 (Tub domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G431340 PE=3 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 2.3e-206
Identity = 362/385 (94.03%), Postives = 367/385 (95.32%), Query Frame = 0

Query: 1   MIGDKKAIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLIPSTKDKQGDE--NA 60
           MIGDKK+IIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENL P+TKDKQ DE   A
Sbjct: 1   MIGDKKSIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLTPNTKDKQRDEPAPA 60

Query: 61  VFCDKENAIPINGFCANSKQQFGDFKSFSTGKALKPSSLRLCMQMNEPDKVFASRLWDAT 120
           V CDKEN IP NGFCANSKQQFGDFKSFS GKALKPSSL LCMQMNEPDKVFASRLWD T
Sbjct: 61  VLCDKENVIPANGFCANSKQQFGDFKSFSNGKALKPSSLHLCMQMNEPDKVFASRLWDGT 120

Query: 121 DSEHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGG 180
           DSEHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGG
Sbjct: 121 DSEHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGG 180

Query: 181 AFYSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGS 240
           AFYSLYTNEGKGRQDRKLAVARH RR GRSEYKIAQSARGILCNADDSFVGSMTTNLVGS
Sbjct: 181 AFYSLYTNEGKGRQDRKLAVARHGRRKGRSEYKIAQSARGILCNADDSFVGSMTTNLVGS 240

Query: 241 KYHIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTN-QV 300
           KY+IWDQGSRLKPIP QS+SLL  VTF PTITTWTGSHRSMKAFIPKHQSMQLKNTN QV
Sbjct: 241 KYYIWDQGSRLKPIPTQSRSLLGAVTFTPTITTWTGSHRSMKAFIPKHQSMQLKNTNQQV 300

Query: 301 QHIAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLT 360
           QHIAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGL+IQTSVKNFQLT
Sbjct: 301 QHIAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLVIQTSVKNFQLT 360

Query: 361 LEENGKQTILQLGRIGKSKYVMDYR 383
           LEENGKQTILQLGRIGKSKYVMDYR
Sbjct: 361 LEENGKQTILQLGRIGKSKYVMDYR 385

BLAST of Lsi02G026480 vs. ExPASy TrEMBL
Match: A0A1S3CEL2 (tubby-like protein 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499857 PE=3 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 3.0e-206
Identity = 357/382 (93.46%), Postives = 365/382 (95.55%), Query Frame = 0

Query: 1   MIGDKKAIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLIPSTKDKQGDENAVF 60
           MIGDKK+IIPRQSSYNSLYLNPLTDL HNRSCSDGGGF+QGKENL P+TKDKQ DE+AV 
Sbjct: 1   MIGDKKSIIPRQSSYNSLYLNPLTDLSHNRSCSDGGGFKQGKENLTPNTKDKQRDESAVL 60

Query: 61  CDKENAIPINGFCANSKQQFGDFKSFSTGKALKPSSLRLCMQMNEPDKVFASRLWDATDS 120
           CDKENAIPINGFCANSKQQFGD KSFSTGKALKPSSL LCMQMNEPDKVFA+RLWD TDS
Sbjct: 61  CDKENAIPINGFCANSKQQFGDLKSFSTGKALKPSSLHLCMQMNEPDKVFATRLWDGTDS 120

Query: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180
           EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF
Sbjct: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180

Query: 181 YSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGSKY 240
           YSLYTNEGKGRQDRKLAVARHRRR GRSEYKIAQS RG+LCNADDSFVGSMTTNLVGSKY
Sbjct: 181 YSLYTNEGKGRQDRKLAVARHRRRKGRSEYKIAQSTRGVLCNADDSFVGSMTTNLVGSKY 240

Query: 241 HIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHI 300
           HIWDQG+RLKP     +SLL  VTFMPTITTWTGSHR MKAFIPKHQSMQLKNTNQVQHI
Sbjct: 241 HIWDQGNRLKP-----RSLLGAVTFMPTITTWTGSHRCMKAFIPKHQSMQLKNTNQVQHI 300

Query: 301 AGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEE 360
           AGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEE
Sbjct: 301 AGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEE 360

Query: 361 NGKQTILQLGRIGKSKYVMDYR 383
           NGKQTILQLGRIGKSKYVMDYR
Sbjct: 361 NGKQTILQLGRIGKSKYVMDYR 377

BLAST of Lsi02G026480 vs. ExPASy TrEMBL
Match: A0A5A7US16 (Tubby-like protein 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001630 PE=3 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 8.7e-206
Identity = 364/401 (90.77%), Postives = 372/401 (92.77%), Query Frame = 0

Query: 1   MIGDKKAIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLIPSTKDKQGDENAVF 60
           MIGDKK+IIPRQSSYNSLYLNPLTDL HNRSCSDGGGF+QGKENL P+TKDKQ DE+AV 
Sbjct: 1   MIGDKKSIIPRQSSYNSLYLNPLTDLSHNRSCSDGGGFKQGKENLTPNTKDKQRDESAVL 60

Query: 61  CDKENAIPINGFCANSKQQFGDFKSFSTGKALKPSSLRLCMQMNEPDKVFASRLWDATDS 120
           CDKENAIPINGFCANSKQQFGD KSFSTGKALKPSSL LCMQMNEPDKVFA+RLWD TDS
Sbjct: 61  CDKENAIPINGFCANSKQQFGDLKSFSTGKALKPSSLHLCMQMNEPDKVFATRLWDGTDS 120

Query: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180
           EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF
Sbjct: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180

Query: 181 YSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGSKY 240
           YSLYTNEGKGRQDRKLAVARHRRR GRSEYKIAQS RG+LCNADDSFVGSMTTNLVGSKY
Sbjct: 181 YSLYTNEGKGRQDRKLAVARHRRRKGRSEYKIAQSTRGVLCNADDSFVGSMTTNLVGSKY 240

Query: 241 HIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTN-QVQH 300
           HIWDQG+RLKP     +SLL  VTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTN QVQH
Sbjct: 241 HIWDQGNRLKP-----RSLLGAVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQQVQH 300

Query: 301 IAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLE 360
           IAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLE
Sbjct: 301 IAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLE 360

Query: 361 ENGKQTILQLGRIGKSKYVMDY-----------RVRVLAFL 390
           ENGKQTILQLGRIGKSKYVMDY           RVRVL FL
Sbjct: 361 ENGKQTILQLGRIGKSKYVMDYSANRDVDVNNDRVRVLGFL 396

BLAST of Lsi02G026480 vs. ExPASy TrEMBL
Match: A0A1S3CE68 (tubby-like protein 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499857 PE=3 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 7.4e-205
Identity = 357/383 (93.21%), Postives = 365/383 (95.30%), Query Frame = 0

Query: 1   MIGDKKAIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLIPSTKDKQGDENAVF 60
           MIGDKK+IIPRQSSYNSLYLNPLTDL HNRSCSDGGGF+QGKENL P+TKDKQ DE+AV 
Sbjct: 1   MIGDKKSIIPRQSSYNSLYLNPLTDLSHNRSCSDGGGFKQGKENLTPNTKDKQRDESAVL 60

Query: 61  CDKENAIPINGFCANSKQQFGDFKSFSTGKALKPSSLRLCMQMNEPDKVFASRLWDATDS 120
           CDKENAIPINGFCANSKQQFGD KSFSTGKALKPSSL LCMQMNEPDKVFA+RLWD TDS
Sbjct: 61  CDKENAIPINGFCANSKQQFGDLKSFSTGKALKPSSLHLCMQMNEPDKVFATRLWDGTDS 120

Query: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180
           EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF
Sbjct: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180

Query: 181 YSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGSKY 240
           YSLYTNEGKGRQDRKLAVARHRRR GRSEYKIAQS RG+LCNADDSFVGSMTTNLVGSKY
Sbjct: 181 YSLYTNEGKGRQDRKLAVARHRRRKGRSEYKIAQSTRGVLCNADDSFVGSMTTNLVGSKY 240

Query: 241 HIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTN-QVQH 300
           HIWDQG+RLKP     +SLL  VTFMPTITTWTGSHR MKAFIPKHQSMQLKNTN QVQH
Sbjct: 241 HIWDQGNRLKP-----RSLLGAVTFMPTITTWTGSHRCMKAFIPKHQSMQLKNTNQQVQH 300

Query: 301 IAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLE 360
           IAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLE
Sbjct: 301 IAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLE 360

Query: 361 ENGKQTILQLGRIGKSKYVMDYR 383
           ENGKQTILQLGRIGKSKYVMDYR
Sbjct: 361 ENGKQTILQLGRIGKSKYVMDYR 378

BLAST of Lsi02G026480 vs. NCBI nr
Match: XP_038899421.1 (tubby-like protein 8 isoform X2 [Benincasa hispida])

HSP 1 Score: 748.0 bits (1930), Expect = 4.4e-212
Identity = 368/382 (96.34%), Postives = 372/382 (97.38%), Query Frame = 0

Query: 1   MIGDKKAIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLIPSTKDKQGDENAVF 60
           MIGDKKAIIPRQSSYNSLYLNPL DLRHNRSCSDGGGFRQGKENLIP+TKDKQ DENA  
Sbjct: 1   MIGDKKAIIPRQSSYNSLYLNPLIDLRHNRSCSDGGGFRQGKENLIPNTKDKQRDENAEC 60

Query: 61  CDKENAIPINGFCANSKQQFGDFKSFSTGKALKPSSLRLCMQMNEPDKVFASRLWDATDS 120
           CDKENAIPIN FCANSKQ+FGDFKSFSTGKALKPSSL LCMQMNEPDKVFASRLWDATDS
Sbjct: 61  CDKENAIPINEFCANSKQKFGDFKSFSTGKALKPSSLHLCMQMNEPDKVFASRLWDATDS 120

Query: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180
           EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF
Sbjct: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180

Query: 181 YSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGSKY 240
           YSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQS RGILCNADDSFVGSMTTNLVGSKY
Sbjct: 181 YSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSTRGILCNADDSFVGSMTTNLVGSKY 240

Query: 241 HIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHI 300
           HIWDQGSRLKPIP + +SLLA VTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHI
Sbjct: 241 HIWDQGSRLKPIPSKPRSLLAAVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHI 300

Query: 301 AGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEE 360
           AGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEE
Sbjct: 301 AGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEE 360

Query: 361 NGKQTILQLGRIGKSKYVMDYR 383
           NGKQTILQLGRIGKSKYVMDYR
Sbjct: 361 NGKQTILQLGRIGKSKYVMDYR 382

BLAST of Lsi02G026480 vs. NCBI nr
Match: XP_038899420.1 (tubby-like protein 8 isoform X1 [Benincasa hispida])

HSP 1 Score: 743.4 bits (1918), Expect = 1.1e-210
Identity = 368/383 (96.08%), Postives = 372/383 (97.13%), Query Frame = 0

Query: 1   MIGDKKAIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLIPSTKDKQGDENAVF 60
           MIGDKKAIIPRQSSYNSLYLNPL DLRHNRSCSDGGGFRQGKENLIP+TKDKQ DENA  
Sbjct: 1   MIGDKKAIIPRQSSYNSLYLNPLIDLRHNRSCSDGGGFRQGKENLIPNTKDKQRDENAEC 60

Query: 61  CDKENAIPINGFCANSKQQFGDFKSFSTGKALKPSSLRLCMQMNEPDKVFASRLWDATDS 120
           CDKENAIPIN FCANSKQ+FGDFKSFSTGKALKPSSL LCMQMNEPDKVFASRLWDATDS
Sbjct: 61  CDKENAIPINEFCANSKQKFGDFKSFSTGKALKPSSLHLCMQMNEPDKVFASRLWDATDS 120

Query: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180
           EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF
Sbjct: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180

Query: 181 YSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGSKY 240
           YSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQS RGILCNADDSFVGSMTTNLVGSKY
Sbjct: 181 YSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSTRGILCNADDSFVGSMTTNLVGSKY 240

Query: 241 HIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTN-QVQH 300
           HIWDQGSRLKPIP + +SLLA VTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTN QVQH
Sbjct: 241 HIWDQGSRLKPIPSKPRSLLAAVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQQVQH 300

Query: 301 IAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLE 360
           IAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLE
Sbjct: 301 IAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLE 360

Query: 361 ENGKQTILQLGRIGKSKYVMDYR 383
           ENGKQTILQLGRIGKSKYVMDYR
Sbjct: 361 ENGKQTILQLGRIGKSKYVMDYR 383

BLAST of Lsi02G026480 vs. NCBI nr
Match: XP_004135888.1 (tubby-like protein 8 isoform X2 [Cucumis sativus])

HSP 1 Score: 732.6 bits (1890), Expect = 1.9e-207
Identity = 362/384 (94.27%), Postives = 367/384 (95.57%), Query Frame = 0

Query: 1   MIGDKKAIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLIPSTKDKQGDE--NA 60
           MIGDKK+IIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENL P+TKDKQ DE   A
Sbjct: 1   MIGDKKSIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLTPNTKDKQRDEPAPA 60

Query: 61  VFCDKENAIPINGFCANSKQQFGDFKSFSTGKALKPSSLRLCMQMNEPDKVFASRLWDAT 120
           V CDKEN IP NGFCANSKQQFGDFKSFS GKALKPSSL LCMQMNEPDKVFASRLWD T
Sbjct: 61  VLCDKENVIPANGFCANSKQQFGDFKSFSNGKALKPSSLHLCMQMNEPDKVFASRLWDGT 120

Query: 121 DSEHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGG 180
           DSEHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGG
Sbjct: 121 DSEHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGG 180

Query: 181 AFYSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGS 240
           AFYSLYTNEGKGRQDRKLAVARH RR GRSEYKIAQSARGILCNADDSFVGSMTTNLVGS
Sbjct: 181 AFYSLYTNEGKGRQDRKLAVARHGRRKGRSEYKIAQSARGILCNADDSFVGSMTTNLVGS 240

Query: 241 KYHIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQ 300
           KY+IWDQGSRLKPIP QS+SLL  VTF PTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQ
Sbjct: 241 KYYIWDQGSRLKPIPTQSRSLLGAVTFTPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQ 300

Query: 301 HIAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTL 360
           HIAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGL+IQTSVKNFQLTL
Sbjct: 301 HIAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLVIQTSVKNFQLTL 360

Query: 361 EENGKQTILQLGRIGKSKYVMDYR 383
           EENGKQTILQLGRIGKSKYVMDYR
Sbjct: 361 EENGKQTILQLGRIGKSKYVMDYR 384

BLAST of Lsi02G026480 vs. NCBI nr
Match: TYK10499.1 (tubby-like protein 8 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 730.7 bits (1885), Expect = 7.3e-207
Identity = 364/400 (91.00%), Postives = 372/400 (93.00%), Query Frame = 0

Query: 1   MIGDKKAIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLIPSTKDKQGDENAVF 60
           MIGDKK+IIPRQSSYNSLYLNPLTDL HNRSCSDGGGF+QGKENL P+TKDKQ DE+AV 
Sbjct: 1   MIGDKKSIIPRQSSYNSLYLNPLTDLSHNRSCSDGGGFKQGKENLTPNTKDKQRDESAVL 60

Query: 61  CDKENAIPINGFCANSKQQFGDFKSFSTGKALKPSSLRLCMQMNEPDKVFASRLWDATDS 120
           CDKENAIPINGFCANSKQQFGD KSFSTGKALKPSSL LCMQMNEPDKVFA+RLWD TDS
Sbjct: 61  CDKENAIPINGFCANSKQQFGDLKSFSTGKALKPSSLHLCMQMNEPDKVFATRLWDGTDS 120

Query: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180
           EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF
Sbjct: 121 EHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAF 180

Query: 181 YSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGSKY 240
           YSLYTNEGKGRQDRKLAVARHRRR GRSEYKIAQS RG+LCNADDSFVGSMTTNLVGSKY
Sbjct: 181 YSLYTNEGKGRQDRKLAVARHRRRKGRSEYKIAQSTRGVLCNADDSFVGSMTTNLVGSKY 240

Query: 241 HIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHI 300
           HIWDQG+RLKP     +SLL  VTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHI
Sbjct: 241 HIWDQGNRLKP-----RSLLGAVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHI 300

Query: 301 AGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEE 360
           AGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEE
Sbjct: 301 AGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEE 360

Query: 361 NGKQTILQLGRIGKSKYVMDY-----------RVRVLAFL 390
           NGKQTILQLGRIGKSKYVMDY           RVRVL FL
Sbjct: 361 NGKQTILQLGRIGKSKYVMDYSANRDVDVNNDRVRVLGFL 395

BLAST of Lsi02G026480 vs. NCBI nr
Match: XP_011659490.1 (tubby-like protein 8 isoform X1 [Cucumis sativus] >KGN45201.1 hypothetical protein Csa_016680 [Cucumis sativus])

HSP 1 Score: 728.0 bits (1878), Expect = 4.7e-206
Identity = 362/385 (94.03%), Postives = 367/385 (95.32%), Query Frame = 0

Query: 1   MIGDKKAIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLIPSTKDKQGDE--NA 60
           MIGDKK+IIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENL P+TKDKQ DE   A
Sbjct: 1   MIGDKKSIIPRQSSYNSLYLNPLTDLRHNRSCSDGGGFRQGKENLTPNTKDKQRDEPAPA 60

Query: 61  VFCDKENAIPINGFCANSKQQFGDFKSFSTGKALKPSSLRLCMQMNEPDKVFASRLWDAT 120
           V CDKEN IP NGFCANSKQQFGDFKSFS GKALKPSSL LCMQMNEPDKVFASRLWD T
Sbjct: 61  VLCDKENVIPANGFCANSKQQFGDFKSFSNGKALKPSSLHLCMQMNEPDKVFASRLWDGT 120

Query: 121 DSEHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGG 180
           DSEHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGG
Sbjct: 121 DSEHSSSLKIWDFSDSEAAPASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGG 180

Query: 181 AFYSLYTNEGKGRQDRKLAVARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGS 240
           AFYSLYTNEGKGRQDRKLAVARH RR GRSEYKIAQSARGILCNADDSFVGSMTTNLVGS
Sbjct: 181 AFYSLYTNEGKGRQDRKLAVARHGRRKGRSEYKIAQSARGILCNADDSFVGSMTTNLVGS 240

Query: 241 KYHIWDQGSRLKPIPIQSKSLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTN-QV 300
           KY+IWDQGSRLKPIP QS+SLL  VTF PTITTWTGSHRSMKAFIPKHQSMQLKNTN QV
Sbjct: 241 KYYIWDQGSRLKPIPTQSRSLLGAVTFTPTITTWTGSHRSMKAFIPKHQSMQLKNTNQQV 300

Query: 301 QHIAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLT 360
           QHIAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGL+IQTSVKNFQLT
Sbjct: 301 QHIAGLPREWEEEKAKVHQLVSKVPQYNKISKQYELDFRDRGRAVSGLVIQTSVKNFQLT 360

Query: 361 LEENGKQTILQLGRIGKSKYVMDYR 383
           LEENGKQTILQLGRIGKSKYVMDYR
Sbjct: 361 LEENGKQTILQLGRIGKSKYVMDYR 385

BLAST of Lsi02G026480 vs. TAIR 10
Match: AT1G16070.1 (tubby like protein 8 )

HSP 1 Score: 389.8 bits (1000), Expect = 2.8e-108
Identity = 193/305 (63.28%), Postives = 245/305 (80.33%), Query Frame = 0

Query: 82  DFKSFSTG--KALKPSSLRLCMQMN-EPDK-VFASRLWDATDSEHSSSLKIWDFSDSEAA 141
           +FKSFSTG   ALK SSL+ CMQ N E DK  F  + W + DSEHSSSLK+W+FSDSEAA
Sbjct: 71  NFKSFSTGGRTALKQSSLQACMQKNSEVDKSSFGMKTWTSVDSEHSSSLKVWEFSDSEAA 130

Query: 142 PASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAFYSLYTNEGKGRQDRKLA 201
           PASSWSTLPNR+LLC+ LP+DVGRCTC+IVKE+SP+GL  G+ YSLYT+EG+GR+DRKLA
Sbjct: 131 PASSWSTLPNRALLCKTLPLDVGRCTCLIVKEQSPEGLSHGSVYSLYTHEGRGRKDRKLA 190

Query: 202 VARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGSKYHIWDQGSRLKPIPIQSK 261
           VA H RRNG+S +++AQ+ +G+LC++D+S+VGSMT NL+GSKY+IWD+G R+  +    K
Sbjct: 191 VAYHSRRNGKSIFRVAQNVKGLLCSSDESYVGSMTANLLGSKYYIWDKGVRVGSVGKMVK 250

Query: 262 SLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHIAGLPREWEEEKAKVHQL 321
            LL+VV F PTITTWTGS+R M+  +PK Q MQ  N  QVQ  + LP +W E K K+ +L
Sbjct: 251 PLLSVVIFTPTITTWTGSYRRMRTLLPKQQPMQKNNNKQVQQASKLPLDWLENKEKIQKL 310

Query: 322 VSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTLEENGKQTILQLGRIGKSKY 381
            S++P YNKISKQ+ELDFRDRGR  +GL IQ+SVKNFQLTL E  +QTILQ+GR+ K++Y
Sbjct: 311 CSRIPHYNKISKQHELDFRDRGR--TGLRIQSSVKNFQLTLTETPRQTILQMGRVDKARY 370

Query: 382 VMDYR 383
           V+D+R
Sbjct: 371 VIDFR 373

BLAST of Lsi02G026480 vs. TAIR 10
Match: AT1G16070.2 (tubby like protein 8 )

HSP 1 Score: 386.3 bits (991), Expect = 3.1e-107
Identity = 193/306 (63.07%), Postives = 246/306 (80.39%), Query Frame = 0

Query: 82  DFKSFSTG--KALKPSSLRLCMQMN-EPDK-VFASRLWDATDSEHSSSLKIWDFSDSEAA 141
           +FKSFSTG   ALK SSL+ CMQ N E DK  F  + W + DSEHSSSLK+W+FSDSEAA
Sbjct: 71  NFKSFSTGGRTALKQSSLQACMQKNSEVDKSSFGMKTWTSVDSEHSSSLKVWEFSDSEAA 130

Query: 142 PASSWSTLPNRSLLCRPLPVDVGRCTCVIVKEKSPDGLHGGAFYSLYTNEGKGRQDRKLA 201
           PASSWSTLPNR+LLC+ LP+DVGRCTC+IVKE+SP+GL  G+ YSLYT+EG+GR+DRKLA
Sbjct: 131 PASSWSTLPNRALLCKTLPLDVGRCTCLIVKEQSPEGLSHGSVYSLYTHEGRGRKDRKLA 190

Query: 202 VARHRRRNGRSEYKIAQSARGILCNADDSFVGSMTTNLVGSKYHIWDQGSRLKPIPIQSK 261
           VA H RRNG+S +++AQ+ +G+LC++D+S+VGSMT NL+GSKY+IWD+G R+  +    K
Sbjct: 191 VAYHSRRNGKSIFRVAQNVKGLLCSSDESYVGSMTANLLGSKYYIWDKGVRVGSVGKMVK 250

Query: 262 SLLAVVTFMPTITTWTGSHRSMKAFIPKHQSMQLKNTNQVQHIAGLPREWEEEKAKVHQL 321
            LL+VV F PTITTWTGS+R M+  +PK Q MQ  N  QVQ  + LP +W E K K+ +L
Sbjct: 251 PLLSVVIFTPTITTWTGSYRRMRTLLPKQQPMQKNNNKQVQQASKLPLDWLENKEKIQKL 310

Query: 322 VSKVPQYNKISKQYELDFRDRGRAVSGLMIQTSVKNFQLTL-EENGKQTILQLGRIGKSK 381
            S++P YNKISKQ+ELDFRDRGR  +GL IQ+SVKNFQLTL +E  +QTILQ+GR+ K++
Sbjct: 311 CSRIPHYNKISKQHELDFRDRGR--TGLRIQSSVKNFQLTLTQETPRQTILQMGRVDKAR 370

Query: 382 YVMDYR 383
           YV+D+R
Sbjct: 371 YVIDFR 374

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9S9M84.0e-10763.28Tubby-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=TULP8 PE=2 SV=1[more]
Q6ETL26.6e-9461.96Tubby-like protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=TULP4 PE=3 SV=2[more]
B0XFQ93.7e-1225.17Protein king tubby 1 OS=Culex quinquefasciatus OX=7176 GN=king-tubby1 PE=3 SV=1[more]
B0WI303.7e-1225.17Protein king tubby 2 OS=Culex quinquefasciatus OX=7176 GN=king-tubby2 PE=3 SV=2[more]
P506073.2e-1125.69Tubby protein homolog OS=Homo sapiens OX=9606 GN=TUB PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CJY13.5e-20791.00Tubby-like protein 8 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0KBM72.3e-20694.03Tub domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G431340 PE=3 SV... [more]
A0A1S3CEL23.0e-20693.46tubby-like protein 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499857 PE=3 SV=... [more]
A0A5A7US168.7e-20690.77Tubby-like protein 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3CE687.4e-20593.21tubby-like protein 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499857 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_038899421.14.4e-21296.34tubby-like protein 8 isoform X2 [Benincasa hispida][more]
XP_038899420.11.1e-21096.08tubby-like protein 8 isoform X1 [Benincasa hispida][more]
XP_004135888.11.9e-20794.27tubby-like protein 8 isoform X2 [Cucumis sativus][more]
TYK10499.17.3e-20791.00tubby-like protein 8 isoform X2 [Cucumis melo var. makuwa][more]
XP_011659490.14.7e-20694.03tubby-like protein 8 isoform X1 [Cucumis sativus] >KGN45201.1 hypothetical prote... [more]
Match NameE-valueIdentityDescription
AT1G16070.12.8e-10863.28tubby like protein 8 [more]
AT1G16070.23.1e-10763.07tubby like protein 8 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000007Tubby, C-terminalPRINTSPR01573SUPERTUBBYcoord: 342..358
score: 47.06
coord: 362..381
score: 35.45
coord: 227..251
score: 24.55
IPR000007Tubby, C-terminalPFAMPF01167Tubcoord: 154..384
e-value: 4.5E-34
score: 118.3
IPR025659Tubby-like, C-terminalGENE3D3.20.90.10Tubby Protein; Chain Acoord: 147..393
e-value: 1.5E-41
score: 144.6
IPR025659Tubby-like, C-terminalSUPERFAMILY54518Tubby C-terminal domain-likecoord: 139..385
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..53
NoneNo IPR availablePANTHERPTHR16517:SF131TUBBY-LIKE PROTEIN 8coord: 1..388
NoneNo IPR availablePANTHERPTHR16517TUBBY-RELATEDcoord: 1..388

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G026480.1Lsi02G026480.1mRNA