Lsi02G026150 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G026150 vs. ExPASy Swiss-Prot
Match: Q9AUE0 (Callose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=CALS1 PE=1 SV=2)

HSP 1 Score: 912.9 bits (2358), Expect = 2.1e-264
Identity = 466/680 (68.53%), Postives = 545/680 (80.15%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRF S+PGAFN CL+P + +D T+ K   A   RKFD++   +D +AA+FAQMWN+II S
Sbjct: 763  SRFQSIPGAFNDCLVPQDNSDDTKKKRFRATFSRKFDQLPSSKDKEAARFAQMWNKIISS 822

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDRELKKRL 122
            FREEDLI++REM+LLLVP W DP+L LI+WPPFLLASKIPIALDMAKDSNG+DRELKKRL
Sbjct: 823  FREEDLISDREMELLLVPYWSDPDLDLIRWPPFLLASKIPIALDMAKDSNGKDRELKKRL 882

Query: 123  TTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRALP 182
              D YM CAVRECYASFKN+IN+LV+GE E  VI +IF+ ID+HI++E LITEL++ ALP
Sbjct: 883  AVDSYMTCAVRECYASFKNLINYLVVGEREGQVINDIFSKIDEHIEKETLITELNLSALP 942

Query: 183  SLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGKDG 242
             LY QFVRLIEY+L N+EEDKDQ+VIVLL+MLE+VTRDI+EEE+ SL+E++H GS+ K  
Sbjct: 943  DLYGQFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSYVKYD 1002

Query: 243  KPRSLD---RLFDKLNFPV-PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFS 302
                L    + F +L FPV  +TEAWKEKI+RLHLLLTVKESAMDVPS+LEARRR++FFS
Sbjct: 1003 VMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFS 1062

Query: 303  NSLFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWT 362
            NSLFM+MPPAPK++NMLSFS+LTPY+SEDVLFS+  LE+ NEDGVSILFYLQKIFP++WT
Sbjct: 1063 NSLFMDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFPDEWT 1122

Query: 363  NFLERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDTAEH 422
            NFLERV+CG+EEEL+  E+LEE+LRLWASYRGQTLTKTVRGMMY+RKALELQAFLD A+ 
Sbjct: 1123 NFLERVKCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKD 1182

Query: 423  QDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYK---------DAVDSPLWA 482
            +                                  ELLKGYK               LWA
Sbjct: 1183 E----------------------------------ELLKGYKALELTSEEASKSGGSLWA 1242

Query: 483  QCQAAVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSR 542
            QCQA  D+KF++VVSCQQY IHKRSGD RAKDILRLM  YPS+RVAYI+E+E+  K+  +
Sbjct: 1243 QCQALADMKFTFVVSCQQYSIHKRSGDQRAKDILRLMTTYPSIRVAYIDEVEQTHKESYK 1302

Query: 543  KTNQKSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFT 602
             T +K YYS L +AA  TK  DS E +Q+LDQ IYR+KLPGPAILGEGKPENQNHAIIFT
Sbjct: 1303 GTEEKIYYSALVKAAPQTKPMDSSESVQTLDQLIYRIKLPGPAILGEGKPENQNHAIIFT 1362

Query: 603  RGECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFM 662
            RGE LQTIDMNQDNYMEEAFKMRNLL+EFL  H GVR PTILGLREHIFTGSVSSLAWFM
Sbjct: 1363 RGEGLQTIDMNQDNYMEEAFKMRNLLQEFLEKHGGVRCPTILGLREHIFTGSVSSLAWFM 1408

Query: 663  SNQENSFVTIGQRLLANPLK 670
            SNQENSFVTIGQR+LA+PLK
Sbjct: 1423 SNQENSFVTIGQRVLASPLK 1408

BLAST of Lsi02G026150 vs. ExPASy Swiss-Prot
Match: Q9SL03 (Callose synthase 2 OS=Arabidopsis thaliana OX=3702 GN=CALS2 PE=2 SV=3)

HSP 1 Score: 911.0 bits (2353), Expect = 8.0e-264
Identity = 469/680 (68.97%), Postives = 548/680 (80.59%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRF SLP AFNACL+P EK++  + KG+MA   RKFD++   +D +AA+FAQMWN+II S
Sbjct: 763  SRFQSLPEAFNACLVPNEKSETPKKKGIMATFTRKFDQVPSSKDKEAARFAQMWNKIISS 822

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDRELKKRL 122
            FREEDLI++REM+LLLVP W D +L LI+WPPFLLASKIPIALDMAKDSNG+DREL KRL
Sbjct: 823  FREEDLISDREMELLLVPYWADRDLDLIRWPPFLLASKIPIALDMAKDSNGKDRELTKRL 882

Query: 123  TTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRALP 182
            + D YM CAVRECYASFKN+INFLV+GE E  VI EIF+ ID+HI++E LI +L++ ALP
Sbjct: 883  SVDSYMTCAVRECYASFKNLINFLVVGEREGQVINEIFSRIDEHIEKETLIKDLNLSALP 942

Query: 183  SLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGKDG 242
             LY QFVRLIEY++ N+EEDKDQ+VIVLL+MLEVVTRDI++EE+ S++ES+H G++ K  
Sbjct: 943  DLYGQFVRLIEYLMENREEDKDQIVIVLLNMLEVVTRDIMDEEVPSMLESTHNGTYVKYD 1002

Query: 243  KPRSLD---RLFDKLNFPV-PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFS 302
                L    + F +L FPV  +TEAWKEKI+RLHLLLTVKESAMDVPS+LEARRR++FFS
Sbjct: 1003 VMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFS 1062

Query: 303  NSLFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWT 362
            NSLFMEMP APK++NMLSFS+LTPYYSEDVLFS+  LEK NEDGVSILFYLQKIFP++WT
Sbjct: 1063 NSLFMEMPDAPKIRNMLSFSVLTPYYSEDVLFSIFGLEKQNEDGVSILFYLQKIFPDEWT 1122

Query: 363  NFLERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDTAEH 422
            NFLERV+CGSEEEL+  EELEE+LRLWASYRGQTLTKTVRGMMY+RKALELQAFLD A+ 
Sbjct: 1123 NFLERVKCGSEEELRAREELEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKD 1182

Query: 423  QDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYK-------DAVDS--PLWA 482
            +                                  EL+KGYK       DA  S   LWA
Sbjct: 1183 E----------------------------------ELMKGYKALELTSEDASKSGTSLWA 1242

Query: 483  QCQAAVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSR 542
            QCQA  D+KF++VVSCQQY + KRSGD RAKDILRLM  YPSLRVAYI+E+E+  K+  +
Sbjct: 1243 QCQALADMKFTFVVSCQQYSVQKRSGDQRAKDILRLMTTYPSLRVAYIDEVEQTHKESYK 1302

Query: 543  KTNQKSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFT 602
              ++K YYS L +AA  TKS DS E +Q+LDQ IYR+KLPGPAILGEGKPENQNH+IIFT
Sbjct: 1303 GADEKIYYSALVKAAPQTKSMDSSESVQTLDQVIYRIKLPGPAILGEGKPENQNHSIIFT 1362

Query: 603  RGECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFM 662
            RGE LQTIDMNQDNYMEEAFKMRNLL+EFL  H GVR PTILGLREHIFTGSVSSLAWFM
Sbjct: 1363 RGEGLQTIDMNQDNYMEEAFKMRNLLQEFLVKHGGVRTPTILGLREHIFTGSVSSLAWFM 1408

Query: 663  SNQENSFVTIGQRLLANPLK 670
            SNQENSFVTIGQR+LA+PLK
Sbjct: 1423 SNQENSFVTIGQRVLASPLK 1408

BLAST of Lsi02G026150 vs. ExPASy Swiss-Prot
Match: Q9LXT9 (Callose synthase 3 OS=Arabidopsis thaliana OX=3702 GN=CALS3 PE=3 SV=3)

HSP 1 Score: 864.0 bits (2231), Expect = 1.1e-249
Identity = 460/676 (68.05%), Postives = 533/676 (78.85%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKF--DEITPDRDDQAAKFAQMWNEII 62
            SRF SLPGAFN  L+P  K +  + KG+ A L   F  D++  +++ +AA+FAQ+WN II
Sbjct: 768  SRFESLPGAFNDRLIPDGK-NQQKKKGIRATLSHNFTEDKVPVNKEKEAARFAQLWNTII 827

Query: 63   ISFREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDRELKK 122
             SFREEDLI++REMDLLLVP W D +L LIQWPPFLLASKIPIALDMAKDSNG+DRELKK
Sbjct: 828  SSFREEDLISDREMDLLLVPYWADRDLDLIQWPPFLLASKIPIALDMAKDSNGKDRELKK 887

Query: 123  RLTTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 182
            R+ +D YM CAVRECYASFKNII F+V G  EK VI+ IF  +D HI   +LI E  M A
Sbjct: 888  RIESDTYMKCAVRECYASFKNIIKFVVQGNREKEVIEIIFAEVDKHIDTGDLIQEYKMSA 947

Query: 183  LPSLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEE--ISSLVESSHGGSF 242
            LPSLYD FV+LI+Y+L NKEED+D VVI+  DMLEVVTRDI+ E+  ISSLV+SSHGG++
Sbjct: 948  LPSLYDHFVKLIKYLLDNKEEDRDHVVILFQDMLEVVTRDIMMEDYNISSLVDSSHGGTW 1007

Query: 243  GKDGKPRSLD-RLF---DKLNFPV-PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRR 302
                 P     +LF     + FP+ P TEAWKEKI+R++LLLT KESAMDVPS+LEARRR
Sbjct: 1008 HGGMIPLEQQYQLFASSGAIRFPIEPVTEAWKEKIKRIYLLLTTKESAMDVPSNLEARRR 1067

Query: 303  ISFFSNSLFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIF 362
            ISFFSNSLFM+MP APKV+NMLSFS+LTPYY+E+VLFS+  LE PNEDGVSILFYLQKIF
Sbjct: 1068 ISFFSNSLFMDMPMAPKVRNMLSFSVLTPYYTEEVLFSLRDLETPNEDGVSILFYLQKIF 1127

Query: 363  PEQWTNFLERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFL 422
            P++W NFLERV+C SEEELK ++ELEE+LRLWASYRGQTLT+TVRGMMY+RKALELQAFL
Sbjct: 1128 PDEWNNFLERVKCLSEEELKESDELEEELRLWASYRGQTLTRTVRGMMYYRKALELQAFL 1187

Query: 423  DTAEHQDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDAVDSPLWAQCQA 482
            D A H+D  L+  Y+                         EL        +  LWAQCQA
Sbjct: 1188 DMAMHED--LMEGYKA-----------------------VELNSENNSRGERSLWAQCQA 1247

Query: 483  AVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSRKTNQ 542
              D+KF+YVVSCQQYGIHKRSGD RA+DILRLM +YPSLRVAYI+E+EEP KDKS+K NQ
Sbjct: 1248 VADMKFTYVVSCQQYGIHKRSGDPRAQDILRLMTRYPSLRVAYIDEVEEPVKDKSKKGNQ 1307

Query: 543  KSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGEC 602
            K YYSVL +     KS D     Q+LDQ IYR++LPGPAILGEGKPENQNHAIIF+RGE 
Sbjct: 1308 KVYYSVLVKV---PKSTDHSTLAQNLDQVIYRIRLPGPAILGEGKPENQNHAIIFSRGEG 1367

Query: 603  LQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQE 662
            LQTIDMNQDNYMEEA KMRNLL+EFLT HDGVR+P+ILGLREHIFTGSVSSLAWFMSNQE
Sbjct: 1368 LQTIDMNQDNYMEEALKMRNLLQEFLTKHDGVRHPSILGLREHIFTGSVSSLAWFMSNQE 1414

Query: 663  NSFVTIGQRLLANPLK 670
             SFVTIGQRLLANPL+
Sbjct: 1428 TSFVTIGQRLLANPLR 1414

BLAST of Lsi02G026150 vs. ExPASy Swiss-Prot
Match: Q9LTG5 (Callose synthase 4 OS=Arabidopsis thaliana OX=3702 GN=CALS4 PE=3 SV=2)

HSP 1 Score: 733.4 bits (1892), Expect = 2.3e-210
Identity = 397/671 (59.17%), Postives = 477/671 (71.09%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRF SLPGAFNACL+P    ++T+ KG+     RK  +I      +A +F+QMWN II S
Sbjct: 749  SRFQSLPGAFNACLIP---NENTKEKGIKLAFSRKCHKIPNTNGKEAKQFSQMWNTIINS 808

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDRELKKRL 122
            FREEDLI+NRE++LLL+  W  P+L  I+WP FLLASKIPIA+D+AK  NG+ RELK  L
Sbjct: 809  FREEDLISNRELELLLMSCWAYPDLDFIRWPIFLLASKIPIAVDIAKKRNGKHRELKNIL 868

Query: 123  TTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRALP 182
              D  M CAVRECYAS K ++N LV G  + ++I  +FTIID HI+++ L+TEL++  LP
Sbjct: 869  AEDNCMSCAVRECYASIKKLLNTLVTGNSDLMLITTVFTIIDTHIEKDTLLTELNLSVLP 928

Query: 183  SLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGKDG 242
             L+  FV+L EY+L NK++DK Q+V VLL +LE+VT+DI+                    
Sbjct: 929  DLHGHFVKLTEYVLQNKDKDKIQIVNVLLKILEMVTKDIL-------------------- 988

Query: 243  KPRSLDRLFDKLNFPVPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNSLF 302
                                  KE+I+RLHLLLTVKESAMDVPS+LEARRR++FFSNSLF
Sbjct: 989  ----------------------KEEIKRLHLLLTVKESAMDVPSNLEARRRLTFFSNSLF 1048

Query: 303  MEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNFLE 362
            MEMP APK+QNMLSFS LTPYYSEDVLFS   LEK N DGVSILFYLQKIFP++W NFLE
Sbjct: 1049 MEMPGAPKIQNMLSFSALTPYYSEDVLFSTFDLEKEN-DGVSILFYLQKIFPDEWKNFLE 1108

Query: 363  RVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDTAEHQDPY 422
            RV+CG+EEEL   + L+E++RLWASYRGQTLTKTVRGMMY++KALELQAF D A  +   
Sbjct: 1109 RVKCGTEEELDAIDYLKEEIRLWASYRGQTLTKTVRGMMYYQKALELQAFFDLANER--- 1168

Query: 423  LLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDA----VDSPLWAQCQAAVDLK 482
                                           EL+KGYK A      S LWA+CQA  D+K
Sbjct: 1169 -------------------------------ELMKGYKSAEASSSGSSLWAECQALADIK 1228

Query: 483  FSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSRKTNQKSYYS 542
            F+YVV+CQQY IHKRSGD RAKDIL LM  YPSLRVAYI+E+E+ +   S+ T++  YYS
Sbjct: 1229 FTYVVACQQYSIHKRSGDQRAKDILTLMTTYPSLRVAYIDEVEQ-THIYSKGTSENFYYS 1288

Query: 543  VLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGECLQTID 602
             L +AA  T S DS +    LDQ IY++KLPGP I+GEGKPENQN+AIIFTRGE LQTID
Sbjct: 1289 ALVKAAPQTYSTDSSDSGHMLDQVIYQIKLPGPPIIGEGKPENQNNAIIFTRGEALQTID 1338

Query: 603  MNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVT 662
            MNQD Y+EEAFKMRNLL+EFL  + GVRYPTILGLREHIFT SVS LAWFMSNQE+SFVT
Sbjct: 1349 MNQDYYIEEAFKMRNLLQEFLEKNGGVRYPTILGLREHIFTRSVSCLAWFMSNQEHSFVT 1338

Query: 663  IGQRLLANPLK 670
            IGQR+LANPLK
Sbjct: 1409 IGQRVLANPLK 1338

BLAST of Lsi02G026150 vs. ExPASy Swiss-Prot
Match: Q3B724 (Callose synthase 5 OS=Arabidopsis thaliana OX=3702 GN=CALS5 PE=1 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 1.1e-193
Identity = 378/684 (55.26%), Postives = 461/684 (67.40%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRF SLPGAFN  L+P   +D TR +G   +L ++F E+T  R  +AAKF+Q+WNEII S
Sbjct: 765  SRFQSLPGAFNTYLVP---SDKTRRRGF--SLSKRFAEVTAARRTEAAKFSQLWNEIISS 824

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDRELKKRL 122
            FREEDLI++REMDLLLVP   DP L LIQWPPFLLASKIPIALDMA     RD +L KR+
Sbjct: 825  FREEDLISDREMDLLLVPYTSDPSLKLIQWPPFLLASKIPIALDMAAQFRTRDSDLWKRI 884

Query: 123  TTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRALP 182
              D+YM CAV ECY SFK++++ LV+GE+EK +I  I   ++ +I + + ++   M  LP
Sbjct: 885  CADEYMKCAVIECYESFKHVLHTLVIGENEKRIIGIIIKEVESNISKNSFLSNFRMAPLP 944

Query: 183  SLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGS----- 242
            +L  +FV L+  +       +D VV++L DMLEVVTRD+++ E   LVE  H        
Sbjct: 945  ALCSKFVELVGILKNADPAKRDTVVLLLQDMLEVVTRDMMQNENRELVELGHTNKESGRQ 1004

Query: 243  --FGKDGKPRSLDRLFDKLNFPVPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRIS 302
               G D KP  L        FP   T  W E+I RLHLLLTVKESAMDVP++LEA+RRI+
Sbjct: 1005 LFAGTDAKPAIL--------FPPVATAQWHEQISRLHLLLTVKESAMDVPTNLEAQRRIA 1064

Query: 303  FFSNSLFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPE 362
            FF+NSLFM+MP AP+V+NMLSFS+LTPYYSE+ ++S N LE  NEDGVS+++YLQKIFP+
Sbjct: 1065 FFTNSLFMDMPRAPRVRNMLSFSVLTPYYSEETVYSKNDLEMENEDGVSVVYYLQKIFPD 1124

Query: 363  QWTNFLERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDT 422
            +WTNFLER+ C  E  +  +EE   QLR W S RGQTL +TVRGMMY+R+AL+LQAFLD 
Sbjct: 1125 EWTNFLERLDCKDETSVLESEENILQLRHWVSLRGQTLFRTVRGMMYYRRALKLQAFLDM 1184

Query: 423  AEHQDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDAVDSP--------- 482
            A                                     E+L GYK A+  P         
Sbjct: 1185 ANE----------------------------------TEILAGYK-AISEPTEEDKKSQR 1244

Query: 483  -LWAQCQAAVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSK 542
             L+ Q +A  DLKF+YV +CQ YG  KRSGD RA DIL LM   PSLRVAYI+E+EE   
Sbjct: 1245 SLYTQLEAVADLKFTYVATCQNYGNQKRSGDRRATDILNLMVNNPSLRVAYIDEVEEREG 1304

Query: 543  DKSRKTNQKSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHA 602
             K     QK +YSVL +A            + +LDQ IYR+KLPGPA +GEGKPENQNHA
Sbjct: 1305 GKV----QKVFYSVLIKA------------VDNLDQEIYRIKLPGPAKIGEGKPENQNHA 1364

Query: 603  IIFTRGECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSL 662
            +IFTRGE LQ IDMNQD+Y+EEA KMRNLLEEF   H GVR PTILG REHIFTGSVSSL
Sbjct: 1365 LIFTRGEALQAIDMNQDHYLEEALKMRNLLEEFNEDH-GVRAPTILGFREHIFTGSVSSL 1383

Query: 663  AWFMSNQENSFVTIGQRLLANPLK 670
            AWFMSNQE SFVTIGQR+LA+PLK
Sbjct: 1425 AWFMSNQETSFVTIGQRVLASPLK 1383

BLAST of Lsi02G026150 vs. ExPASy TrEMBL
Match: A0A0A0K8F7 (1,3-beta-glucan synthase OS=Cucumis sativus OX=3659 GN=Csa_7G429550 PE=3 SV=1)

HSP 1 Score: 1182.2 bits (3057), Expect = 0.0e+00
Identity = 605/679 (89.10%), Postives = 621/679 (91.46%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRFLSLPGAFNACL+PVEK DHTR KGLMAN GRKFDEITPDRDDQAAKFAQMWNEIIIS
Sbjct: 761  SRFLSLPGAFNACLIPVEKKDHTRKKGLMANFGRKFDEITPDRDDQAAKFAQMWNEIIIS 820

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDR--ELKK 122
            FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDS GRDR  ELKK
Sbjct: 821  FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSYGRDREGELKK 880

Query: 123  RLTTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 182
            RL+TDKYMLCAV+ECYASFKNIINFLVLGEHEKLVIKEIFTIID+HIK ENLI ELDMRA
Sbjct: 881  RLSTDKYMLCAVQECYASFKNIINFLVLGEHEKLVIKEIFTIIDEHIKAENLIVELDMRA 940

Query: 183  LPSLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGK 242
            LPSLY+QFVRLIEYMLTNK EDKDQVVIVLLDMLEVVTRDII+EEISSLVESSHGGSFGK
Sbjct: 941  LPSLYEQFVRLIEYMLTNKVEDKDQVVIVLLDMLEVVTRDIIDEEISSLVESSHGGSFGK 1000

Query: 243  DGKPRSLDRLFDKLNFPVPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 302
            DGKPRSLDRLFDKLNFP+PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS
Sbjct: 1001 DGKPRSLDRLFDKLNFPIPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 1060

Query: 303  LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 362
            LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF
Sbjct: 1061 LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 1120

Query: 363  LERVQCGSEEELKN----------NEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQ 422
            LERVQC +EEEL+N           +EL+E+LRLWASYRGQTLTKTVRGMMY+RKALELQ
Sbjct: 1121 LERVQCANEEELRNKLELEEIQQKTDELKEELRLWASYRGQTLTKTVRGMMYYRKALELQ 1180

Query: 423  AFLDTAEHQDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDAVDSPLWAQ 482
            AFLDTAEHQD                                  LLKGYKDAVDSPLWAQ
Sbjct: 1181 AFLDTAEHQD----------------------------------LLKGYKDAVDSPLWAQ 1240

Query: 483  CQAAVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSRK 542
            CQAAVD+KFSYVVSCQQYGIHKRSGDARAKDIL+LM KYPSLRVAYIEELEEPSKDKSRK
Sbjct: 1241 CQAAVDMKFSYVVSCQQYGIHKRSGDARAKDILKLMTKYPSLRVAYIEELEEPSKDKSRK 1300

Query: 543  TNQKSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTR 602
            TNQKSYYSVLARAALPTKSKDS E +QSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTR
Sbjct: 1301 TNQKSYYSVLARAALPTKSKDSTESVQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTR 1360

Query: 603  GECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMS 662
            GECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMS
Sbjct: 1361 GECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMS 1405

Query: 663  NQENSFVTIGQRLLANPLK 670
            NQENSFVTIGQRLLANPLK
Sbjct: 1421 NQENSFVTIGQRLLANPLK 1405

BLAST of Lsi02G026150 vs. ExPASy TrEMBL
Match: A0A1S3CEN5 (1,3-beta-glucan synthase OS=Cucumis melo OX=3656 GN=LOC103499883 PE=3 SV=1)

HSP 1 Score: 1179.9 bits (3051), Expect = 0.0e+00
Identity = 603/679 (88.81%), Postives = 620/679 (91.31%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRFLSLPGAFNACL+PVEK DHTR KGLMAN GRKFDEITPDRDDQAAKFAQMWNEIIIS
Sbjct: 761  SRFLSLPGAFNACLIPVEKKDHTRKKGLMANFGRKFDEITPDRDDQAAKFAQMWNEIIIS 820

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDR--ELKK 122
            FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDS GRDR  ELKK
Sbjct: 821  FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSYGRDREGELKK 880

Query: 123  RLTTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 182
            RL+TDKYMLCAV+ECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIK ENLI ELDMRA
Sbjct: 881  RLSTDKYMLCAVQECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKAENLIVELDMRA 940

Query: 183  LPSLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGK 242
            LPSLY+QFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDII+EEISSLVESSHGGSFGK
Sbjct: 941  LPSLYEQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIDEEISSLVESSHGGSFGK 1000

Query: 243  DGKPRSLDRLFDKLNFPVPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 302
            DGKPRSLDRLFDKLNFP+PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS
Sbjct: 1001 DGKPRSLDRLFDKLNFPIPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 1060

Query: 303  LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 362
            LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKI+PEQWTNF
Sbjct: 1061 LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIYPEQWTNF 1120

Query: 363  LERVQCGSEEELKN----------NEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQ 422
            LERVQC +EEEL+N           +EL+E+LRLWASYRGQTLTKTVRGMMY+RKALELQ
Sbjct: 1121 LERVQCANEEELRNKLELEDIHNKTDELKEELRLWASYRGQTLTKTVRGMMYYRKALELQ 1180

Query: 423  AFLDTAEHQDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDAVDSPLWAQ 482
            AFLDTAEHQD                                  LLKGYKDAVDSPLWAQ
Sbjct: 1181 AFLDTAEHQD----------------------------------LLKGYKDAVDSPLWAQ 1240

Query: 483  CQAAVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSRK 542
            CQAAVD+KFSYVVSCQQYGIHKRSGDARAKDIL+LM KYPSLRVAYIEELEEPSKDKSRK
Sbjct: 1241 CQAAVDMKFSYVVSCQQYGIHKRSGDARAKDILKLMTKYPSLRVAYIEELEEPSKDKSRK 1300

Query: 543  TNQKSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTR 602
            +NQKSYYSVLARAA PTKSKDS E +QSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTR
Sbjct: 1301 SNQKSYYSVLARAAFPTKSKDSTESVQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTR 1360

Query: 603  GECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMS 662
            GECLQTIDMNQDNYMEEAFKMRNLLEEFLT HDGVRYPTILGLREHIFTGSVSSLAWFMS
Sbjct: 1361 GECLQTIDMNQDNYMEEAFKMRNLLEEFLTKHDGVRYPTILGLREHIFTGSVSSLAWFMS 1405

Query: 663  NQENSFVTIGQRLLANPLK 670
            NQENSFVTIGQRLLANPLK
Sbjct: 1421 NQENSFVTIGQRLLANPLK 1405

BLAST of Lsi02G026150 vs. ExPASy TrEMBL
Match: A0A6J1GN91 (1,3-beta-glucan synthase OS=Cucurbita moschata OX=3662 GN=LOC111455968 PE=3 SV=1)

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 592/667 (88.76%), Postives = 614/667 (92.05%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRFLSLPGAFNACL+PVEK DHTR KGLMANL RKFDEITPDRDDQAAKFAQMWNEIIIS
Sbjct: 761  SRFLSLPGAFNACLIPVEKKDHTRKKGLMANLSRKFDEITPDRDDQAAKFAQMWNEIIIS 820

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDRELKKRL 122
            FREEDLI+NREMDLLLVPNWIDP+L+LIQWPPFLLASKIPIALDMAKDSN RDRELKKRL
Sbjct: 821  FREEDLISNREMDLLLVPNWIDPDLALIQWPPFLLASKIPIALDMAKDSNRRDRELKKRL 880

Query: 123  TTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRALP 182
            +TDKYMLCAVRECYASFK+IINFLVLGEHEKLVIKEIFTIIDDHIKE+NLI ELDM+ALP
Sbjct: 881  STDKYMLCAVRECYASFKSIINFLVLGEHEKLVIKEIFTIIDDHIKEDNLIVELDMKALP 940

Query: 183  SLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGKDG 242
            +LY QFVRLIEYM  NKEEDKDQVVIVLLDMLEV TRDII+EEISSLVESSHGGS GKDG
Sbjct: 941  NLYYQFVRLIEYMQNNKEEDKDQVVIVLLDMLEVGTRDIIDEEISSLVESSHGGSSGKDG 1000

Query: 243  KPRSLDRLFDKLNFPVPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNSLF 302
            K RSLDRLFDKLNFP+PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNSLF
Sbjct: 1001 KSRSLDRLFDKLNFPIPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNSLF 1060

Query: 303  MEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNFLE 362
            MEMPPAPKVQNMLSFS+LTPYY EDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNFLE
Sbjct: 1061 MEMPPAPKVQNMLSFSVLTPYYDEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNFLE 1120

Query: 363  RVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDTAEHQDPY 422
            RVQC SEEEL+NNEELEEQLRLWASYRGQTLTKTVRGMMY+RKALELQAFLDTAEH+   
Sbjct: 1121 RVQCNSEEELRNNEELEEQLRLWASYRGQTLTKTVRGMMYYRKALELQAFLDTAEHR--- 1180

Query: 423  LLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDAVDSPLWAQCQAAVDLKFSYV 482
                                           ELLKGYKD+VDSPLWAQCQA+VD+KFSYV
Sbjct: 1181 -------------------------------ELLKGYKDSVDSPLWAQCQASVDMKFSYV 1240

Query: 483  VSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSRKTNQKSYYSVLAR 542
            VSCQ+YGIHKRSGD RAKDILRLMAKYPSLRVAYIEELEEP K+KSRKTNQKSYYSVLAR
Sbjct: 1241 VSCQKYGIHKRSGDPRAKDILRLMAKYPSLRVAYIEELEEPCKNKSRKTNQKSYYSVLAR 1300

Query: 543  AALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMNQD 602
            AALPTKSKD IE +Q+LDQ IYRVKLPGPAILGEGKPENQNHAIIFTRGE LQTIDMNQD
Sbjct: 1301 AALPTKSKDPIEQVQNLDQIIYRVKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQD 1360

Query: 603  NYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQR 662
            NYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQR
Sbjct: 1361 NYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQR 1393

Query: 663  LLANPLK 670
            LLANPLK
Sbjct: 1421 LLANPLK 1393

BLAST of Lsi02G026150 vs. ExPASy TrEMBL
Match: A0A6J1JU28 (1,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111488369 PE=3 SV=1)

HSP 1 Score: 1166.0 bits (3015), Expect = 0.0e+00
Identity = 592/667 (88.76%), Postives = 613/667 (91.90%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRFLSLPGAFNACL+PVEK DHTR KGLMANL RKFDEITPDRDDQAAKFAQMWNEIIIS
Sbjct: 761  SRFLSLPGAFNACLIPVEKKDHTRKKGLMANLSRKFDEITPDRDDQAAKFAQMWNEIIIS 820

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDRELKKRL 122
            FREEDLI+NREMDLLLVPNWIDP+L LIQWPPFLLASKIPIALDMAKDSN RDRELKKRL
Sbjct: 821  FREEDLISNREMDLLLVPNWIDPDLVLIQWPPFLLASKIPIALDMAKDSNRRDRELKKRL 880

Query: 123  TTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRALP 182
            +TDKYMLCAVRECYASFK+IINFLVLGEHEKLVIKEIFTIIDDHIKE+NLI ELDM+ALP
Sbjct: 881  STDKYMLCAVRECYASFKSIINFLVLGEHEKLVIKEIFTIIDDHIKEDNLIVELDMKALP 940

Query: 183  SLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGKDG 242
            +LY QFVRLIEYM  NKEEDKDQVVIVLLDMLEV TRDII+EEISSLVESSHGGS GKDG
Sbjct: 941  NLYHQFVRLIEYMQNNKEEDKDQVVIVLLDMLEVGTRDIIDEEISSLVESSHGGSSGKDG 1000

Query: 243  KPRSLDRLFDKLNFPVPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNSLF 302
            K RSLDRLFDKLNFP+PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNSLF
Sbjct: 1001 KSRSLDRLFDKLNFPIPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNSLF 1060

Query: 303  MEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNFLE 362
            MEMPPAPKVQNMLSFS+LTPYY EDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNFLE
Sbjct: 1061 MEMPPAPKVQNMLSFSVLTPYYDEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNFLE 1120

Query: 363  RVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDTAEHQDPY 422
            RVQC SEEEL+NNEELEEQLRLWASYRGQTLTKTVRGMMY+RKALELQAFLDTAEH+   
Sbjct: 1121 RVQCNSEEELRNNEELEEQLRLWASYRGQTLTKTVRGMMYYRKALELQAFLDTAEHR--- 1180

Query: 423  LLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDAVDSPLWAQCQAAVDLKFSYV 482
                                           ELLKGYKD+VDSPLWAQCQA+VD+KFSYV
Sbjct: 1181 -------------------------------ELLKGYKDSVDSPLWAQCQASVDMKFSYV 1240

Query: 483  VSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSRKTNQKSYYSVLAR 542
            VSCQ+YGIHKRSGD RAKDILRLMAKYPSLRVAYIEELEEP K+KSRKTNQKSYYSVLAR
Sbjct: 1241 VSCQKYGIHKRSGDPRAKDILRLMAKYPSLRVAYIEELEEPCKNKSRKTNQKSYYSVLAR 1300

Query: 543  AALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMNQD 602
            AALPTKSKD IE +Q+LDQ IYRVKLPGPAILGEGKPENQNHAIIFTRGE LQTIDMNQD
Sbjct: 1301 AALPTKSKDPIEQVQNLDQIIYRVKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQD 1360

Query: 603  NYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQR 662
            NYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQR
Sbjct: 1361 NYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQR 1393

Query: 663  LLANPLK 670
            LLANPLK
Sbjct: 1421 LLANPLK 1393

BLAST of Lsi02G026150 vs. ExPASy TrEMBL
Match: A0A5A7UU13 (1,3-beta-glucan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001200 PE=3 SV=1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 603/705 (85.53%), Postives = 620/705 (87.94%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRFLSLPGAFNACL+PVEK DHTR KGLMAN GRKFDEITPDRDDQAAKFAQMWNEIIIS
Sbjct: 762  SRFLSLPGAFNACLIPVEKKDHTRKKGLMANFGRKFDEITPDRDDQAAKFAQMWNEIIIS 821

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDR--ELKK 122
            FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDS GRDR  ELKK
Sbjct: 822  FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSYGRDREGELKK 881

Query: 123  RLTTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 182
            RL+TDKYMLCAV+ECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIK ENLI ELDMRA
Sbjct: 882  RLSTDKYMLCAVQECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKAENLIVELDMRA 941

Query: 183  LPSLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGK 242
            LPSLY+QFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDII+EEISSLVESSHGGSFGK
Sbjct: 942  LPSLYEQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIDEEISSLVESSHGGSFGK 1001

Query: 243  DGKPRSLDRLFDKLNFPVPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 302
            DGKPRSLDRLFDKLNFP+PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS
Sbjct: 1002 DGKPRSLDRLFDKLNFPIPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 1061

Query: 303  LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFP------ 362
            LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKI+P      
Sbjct: 1062 LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIYPGFFFLT 1121

Query: 363  --------------------EQWTNFLERVQCGSEEELKN----------NEELEEQLRL 422
                                EQWTNFLERVQC +EEEL+N           +EL+E+LRL
Sbjct: 1122 FSWIYYVLLKFHSIRLIIPIEQWTNFLERVQCANEEELRNKLELEDIHNKTDELKEELRL 1181

Query: 423  WASYRGQTLTKTVRGMMYHRKALELQAFLDTAEHQDPYLLYLYEKKYVWRVRGRYKYKKS 482
            WASYRGQTLTKTVRGMMY+RKALELQAFLDTAEHQD                        
Sbjct: 1182 WASYRGQTLTKTVRGMMYYRKALELQAFLDTAEHQD------------------------ 1241

Query: 483  FYKFKCGYPELLKGYKDAVDSPLWAQCQAAVDLKFSYVVSCQQYGIHKRSGDARAKDILR 542
                      LLKGYKDAVDSPLWAQCQAAVD+KFSYVVSCQQYGIHKRSGDARAKDIL+
Sbjct: 1242 ----------LLKGYKDAVDSPLWAQCQAAVDMKFSYVVSCQQYGIHKRSGDARAKDILK 1301

Query: 543  LMAKYPSLRVAYIEELEEPSKDKSRKTNQKSYYSVLARAALPTKSKDSIEPIQSLDQTIY 602
            LM KYPSLRVAYIEELEEPSKDKSRK+NQKSYYSVLARAA PTKSKDS E +QSLDQTIY
Sbjct: 1302 LMTKYPSLRVAYIEELEEPSKDKSRKSNQKSYYSVLARAAFPTKSKDSTESVQSLDQTIY 1361

Query: 603  RVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDG 662
            RVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMNQDNYMEEAFKMRNLLEEFLT HDG
Sbjct: 1362 RVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMNQDNYMEEAFKMRNLLEEFLTKHDG 1421

Query: 663  VRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQRLLANPLK 670
            VRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQRLLANPLK
Sbjct: 1422 VRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQRLLANPLK 1432

BLAST of Lsi02G026150 vs. NCBI nr
Match: XP_038898164.1 (callose synthase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 615/669 (91.93%), Postives = 625/669 (93.42%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRFLSLPGAFNACL+PVEK DHTR KGLMAN GRKFDEITPDRDDQAAKFAQMWNEIIIS
Sbjct: 479  SRFLSLPGAFNACLIPVEKKDHTRKKGLMANFGRKFDEITPDRDDQAAKFAQMWNEIIIS 538

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDR--ELKK 122
            FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDR  ELKK
Sbjct: 539  FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDREGELKK 598

Query: 123  RLTTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 182
            RL+TDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA
Sbjct: 599  RLSTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 658

Query: 183  LPSLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGK 242
            LPSLY+QFVRLIEYML NKEEDKDQVVIVLLDMLEVVTRDII+EEISSLVESSHGGSFGK
Sbjct: 659  LPSLYEQFVRLIEYMLANKEEDKDQVVIVLLDMLEVVTRDIIDEEISSLVESSHGGSFGK 718

Query: 243  DGKPRSLDRLFDKLNFPVPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 302
            DGKPR+LDRLFDKLNFP+PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS
Sbjct: 719  DGKPRALDRLFDKLNFPIPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 778

Query: 303  LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 362
            LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF
Sbjct: 779  LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 838

Query: 363  LERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDTAEHQD 422
            LERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMY+RKALELQAFLDTAEHQ 
Sbjct: 839  LERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYYRKALELQAFLDTAEHQ- 898

Query: 423  PYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDAVDSPLWAQCQAAVDLKFS 482
                                             ELLKGYKDAVDSPLWAQCQAAVD+KFS
Sbjct: 899  ---------------------------------ELLKGYKDAVDSPLWAQCQAAVDMKFS 958

Query: 483  YVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSRKTNQKSYYSVL 542
            YVVSCQQYGIHKRSG A AKDILRLM KYPSLRVAYIEELEEPSKDKSRKTNQKSYYSVL
Sbjct: 959  YVVSCQQYGIHKRSGAAPAKDILRLMTKYPSLRVAYIEELEEPSKDKSRKTNQKSYYSVL 1018

Query: 543  ARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMN 602
            ARAA+PTKSKDS EP+QSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMN
Sbjct: 1019 ARAAMPTKSKDSSEPVQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMN 1078

Query: 603  QDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIG 662
            QDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIG
Sbjct: 1079 QDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIG 1113

Query: 663  QRLLANPLK 670
            QRLLANPLK
Sbjct: 1139 QRLLANPLK 1113

BLAST of Lsi02G026150 vs. NCBI nr
Match: XP_038898161.1 (callose synthase 1-like isoform X1 [Benincasa hispida] >XP_038898162.1 callose synthase 1-like isoform X1 [Benincasa hispida] >XP_038898163.1 callose synthase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 615/669 (91.93%), Postives = 625/669 (93.42%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRFLSLPGAFNACL+PVEK DHTR KGLMAN GRKFDEITPDRDDQAAKFAQMWNEIIIS
Sbjct: 761  SRFLSLPGAFNACLIPVEKKDHTRKKGLMANFGRKFDEITPDRDDQAAKFAQMWNEIIIS 820

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDR--ELKK 122
            FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDR  ELKK
Sbjct: 821  FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDREGELKK 880

Query: 123  RLTTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 182
            RL+TDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA
Sbjct: 881  RLSTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 940

Query: 183  LPSLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGK 242
            LPSLY+QFVRLIEYML NKEEDKDQVVIVLLDMLEVVTRDII+EEISSLVESSHGGSFGK
Sbjct: 941  LPSLYEQFVRLIEYMLANKEEDKDQVVIVLLDMLEVVTRDIIDEEISSLVESSHGGSFGK 1000

Query: 243  DGKPRSLDRLFDKLNFPVPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 302
            DGKPR+LDRLFDKLNFP+PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS
Sbjct: 1001 DGKPRALDRLFDKLNFPIPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 1060

Query: 303  LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 362
            LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF
Sbjct: 1061 LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 1120

Query: 363  LERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDTAEHQD 422
            LERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMY+RKALELQAFLDTAEHQ 
Sbjct: 1121 LERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYYRKALELQAFLDTAEHQ- 1180

Query: 423  PYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDAVDSPLWAQCQAAVDLKFS 482
                                             ELLKGYKDAVDSPLWAQCQAAVD+KFS
Sbjct: 1181 ---------------------------------ELLKGYKDAVDSPLWAQCQAAVDMKFS 1240

Query: 483  YVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSRKTNQKSYYSVL 542
            YVVSCQQYGIHKRSG A AKDILRLM KYPSLRVAYIEELEEPSKDKSRKTNQKSYYSVL
Sbjct: 1241 YVVSCQQYGIHKRSGAAPAKDILRLMTKYPSLRVAYIEELEEPSKDKSRKTNQKSYYSVL 1300

Query: 543  ARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMN 602
            ARAA+PTKSKDS EP+QSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMN
Sbjct: 1301 ARAAMPTKSKDSSEPVQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMN 1360

Query: 603  QDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIG 662
            QDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIG
Sbjct: 1361 QDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIG 1395

Query: 663  QRLLANPLK 670
            QRLLANPLK
Sbjct: 1421 QRLLANPLK 1395

BLAST of Lsi02G026150 vs. NCBI nr
Match: XP_038898165.1 (callose synthase 1-like isoform X3 [Benincasa hispida])

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 615/669 (91.93%), Postives = 625/669 (93.42%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRFLSLPGAFNACL+PVEK DHTR KGLMAN GRKFDEITPDRDDQAAKFAQMWNEIIIS
Sbjct: 761  SRFLSLPGAFNACLIPVEKKDHTRKKGLMANFGRKFDEITPDRDDQAAKFAQMWNEIIIS 820

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDR--ELKK 122
            FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDR  ELKK
Sbjct: 821  FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDREGELKK 880

Query: 123  RLTTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 182
            RL+TDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA
Sbjct: 881  RLSTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 940

Query: 183  LPSLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGK 242
            LPSLY+QFVRLIEYML NKEEDKDQVVIVLLDMLEVVTRDII+EEISSLVESSHGGSFGK
Sbjct: 941  LPSLYEQFVRLIEYMLANKEEDKDQVVIVLLDMLEVVTRDIIDEEISSLVESSHGGSFGK 1000

Query: 243  DGKPRSLDRLFDKLNFPVPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 302
            DGKPR+LDRLFDKLNFP+PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS
Sbjct: 1001 DGKPRALDRLFDKLNFPIPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 1060

Query: 303  LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 362
            LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF
Sbjct: 1061 LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 1120

Query: 363  LERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDTAEHQD 422
            LERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMY+RKALELQAFLDTAEHQ 
Sbjct: 1121 LERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYYRKALELQAFLDTAEHQ- 1180

Query: 423  PYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDAVDSPLWAQCQAAVDLKFS 482
                                             ELLKGYKDAVDSPLWAQCQAAVD+KFS
Sbjct: 1181 ---------------------------------ELLKGYKDAVDSPLWAQCQAAVDMKFS 1240

Query: 483  YVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSRKTNQKSYYSVL 542
            YVVSCQQYGIHKRSG A AKDILRLM KYPSLRVAYIEELEEPSKDKSRKTNQKSYYSVL
Sbjct: 1241 YVVSCQQYGIHKRSGAAPAKDILRLMTKYPSLRVAYIEELEEPSKDKSRKTNQKSYYSVL 1300

Query: 543  ARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMN 602
            ARAA+PTKSKDS EP+QSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMN
Sbjct: 1301 ARAAMPTKSKDSSEPVQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGECLQTIDMN 1360

Query: 603  QDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIG 662
            QDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIG
Sbjct: 1361 QDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQENSFVTIG 1395

Query: 663  QRLLANPLK 670
            QRLLANPLK
Sbjct: 1421 QRLLANPLK 1395

BLAST of Lsi02G026150 vs. NCBI nr
Match: KAE8646412.1 (hypothetical protein Csa_016046 [Cucumis sativus])

HSP 1 Score: 1182.2 bits (3057), Expect = 0.0e+00
Identity = 605/679 (89.10%), Postives = 621/679 (91.46%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRFLSLPGAFNACL+PVEK DHTR KGLMAN GRKFDEITPDRDDQAAKFAQMWNEIIIS
Sbjct: 745  SRFLSLPGAFNACLIPVEKKDHTRKKGLMANFGRKFDEITPDRDDQAAKFAQMWNEIIIS 804

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDR--ELKK 122
            FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDS GRDR  ELKK
Sbjct: 805  FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSYGRDREGELKK 864

Query: 123  RLTTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 182
            RL+TDKYMLCAV+ECYASFKNIINFLVLGEHEKLVIKEIFTIID+HIK ENLI ELDMRA
Sbjct: 865  RLSTDKYMLCAVQECYASFKNIINFLVLGEHEKLVIKEIFTIIDEHIKAENLIVELDMRA 924

Query: 183  LPSLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGK 242
            LPSLY+QFVRLIEYMLTNK EDKDQVVIVLLDMLEVVTRDII+EEISSLVESSHGGSFGK
Sbjct: 925  LPSLYEQFVRLIEYMLTNKVEDKDQVVIVLLDMLEVVTRDIIDEEISSLVESSHGGSFGK 984

Query: 243  DGKPRSLDRLFDKLNFPVPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 302
            DGKPRSLDRLFDKLNFP+PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS
Sbjct: 985  DGKPRSLDRLFDKLNFPIPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 1044

Query: 303  LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 362
            LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF
Sbjct: 1045 LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 1104

Query: 363  LERVQCGSEEELKN----------NEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQ 422
            LERVQC +EEEL+N           +EL+E+LRLWASYRGQTLTKTVRGMMY+RKALELQ
Sbjct: 1105 LERVQCANEEELRNKLELEEIQQKTDELKEELRLWASYRGQTLTKTVRGMMYYRKALELQ 1164

Query: 423  AFLDTAEHQDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDAVDSPLWAQ 482
            AFLDTAEHQD                                  LLKGYKDAVDSPLWAQ
Sbjct: 1165 AFLDTAEHQD----------------------------------LLKGYKDAVDSPLWAQ 1224

Query: 483  CQAAVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSRK 542
            CQAAVD+KFSYVVSCQQYGIHKRSGDARAKDIL+LM KYPSLRVAYIEELEEPSKDKSRK
Sbjct: 1225 CQAAVDMKFSYVVSCQQYGIHKRSGDARAKDILKLMTKYPSLRVAYIEELEEPSKDKSRK 1284

Query: 543  TNQKSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTR 602
            TNQKSYYSVLARAALPTKSKDS E +QSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTR
Sbjct: 1285 TNQKSYYSVLARAALPTKSKDSTESVQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTR 1344

Query: 603  GECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMS 662
            GECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMS
Sbjct: 1345 GECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMS 1389

Query: 663  NQENSFVTIGQRLLANPLK 670
            NQENSFVTIGQRLLANPLK
Sbjct: 1405 NQENSFVTIGQRLLANPLK 1389

BLAST of Lsi02G026150 vs. NCBI nr
Match: XP_031744397.1 (callose synthase 1 [Cucumis sativus] >XP_031744398.1 callose synthase 1 [Cucumis sativus])

HSP 1 Score: 1182.2 bits (3057), Expect = 0.0e+00
Identity = 605/679 (89.10%), Postives = 621/679 (91.46%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRFLSLPGAFNACL+PVEK DHTR KGLMAN GRKFDEITPDRDDQAAKFAQMWNEIIIS
Sbjct: 761  SRFLSLPGAFNACLIPVEKKDHTRKKGLMANFGRKFDEITPDRDDQAAKFAQMWNEIIIS 820

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDR--ELKK 122
            FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDS GRDR  ELKK
Sbjct: 821  FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSYGRDREGELKK 880

Query: 123  RLTTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 182
            RL+TDKYMLCAV+ECYASFKNIINFLVLGEHEKLVIKEIFTIID+HIK ENLI ELDMRA
Sbjct: 881  RLSTDKYMLCAVQECYASFKNIINFLVLGEHEKLVIKEIFTIIDEHIKAENLIVELDMRA 940

Query: 183  LPSLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGK 242
            LPSLY+QFVRLIEYMLTNK EDKDQVVIVLLDMLEVVTRDII+EEISSLVESSHGGSFGK
Sbjct: 941  LPSLYEQFVRLIEYMLTNKVEDKDQVVIVLLDMLEVVTRDIIDEEISSLVESSHGGSFGK 1000

Query: 243  DGKPRSLDRLFDKLNFPVPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 302
            DGKPRSLDRLFDKLNFP+PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS
Sbjct: 1001 DGKPRSLDRLFDKLNFPIPETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFSNS 1060

Query: 303  LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 362
            LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF
Sbjct: 1061 LFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWTNF 1120

Query: 363  LERVQCGSEEELKN----------NEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQ 422
            LERVQC +EEEL+N           +EL+E+LRLWASYRGQTLTKTVRGMMY+RKALELQ
Sbjct: 1121 LERVQCANEEELRNKLELEEIQQKTDELKEELRLWASYRGQTLTKTVRGMMYYRKALELQ 1180

Query: 423  AFLDTAEHQDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDAVDSPLWAQ 482
            AFLDTAEHQD                                  LLKGYKDAVDSPLWAQ
Sbjct: 1181 AFLDTAEHQD----------------------------------LLKGYKDAVDSPLWAQ 1240

Query: 483  CQAAVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSRK 542
            CQAAVD+KFSYVVSCQQYGIHKRSGDARAKDIL+LM KYPSLRVAYIEELEEPSKDKSRK
Sbjct: 1241 CQAAVDMKFSYVVSCQQYGIHKRSGDARAKDILKLMTKYPSLRVAYIEELEEPSKDKSRK 1300

Query: 543  TNQKSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTR 602
            TNQKSYYSVLARAALPTKSKDS E +QSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTR
Sbjct: 1301 TNQKSYYSVLARAALPTKSKDSTESVQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTR 1360

Query: 603  GECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMS 662
            GECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMS
Sbjct: 1361 GECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMS 1405

Query: 663  NQENSFVTIGQRLLANPLK 670
            NQENSFVTIGQRLLANPLK
Sbjct: 1421 NQENSFVTIGQRLLANPLK 1405

BLAST of Lsi02G026150 vs. TAIR 10
Match: AT1G05570.1 (callose synthase 1 )

HSP 1 Score: 912.9 bits (2358), Expect = 1.5e-265
Identity = 466/680 (68.53%), Postives = 545/680 (80.15%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRF S+PGAFN CL+P + +D T+ K   A   RKFD++   +D +AA+FAQMWN+II S
Sbjct: 763  SRFQSIPGAFNDCLVPQDNSDDTKKKRFRATFSRKFDQLPSSKDKEAARFAQMWNKIISS 822

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDRELKKRL 122
            FREEDLI++REM+LLLVP W DP+L LI+WPPFLLASKIPIALDMAKDSNG+DRELKKRL
Sbjct: 823  FREEDLISDREMELLLVPYWSDPDLDLIRWPPFLLASKIPIALDMAKDSNGKDRELKKRL 882

Query: 123  TTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRALP 182
              D YM CAVRECYASFKN+IN+LV+GE E  VI +IF+ ID+HI++E LITEL++ ALP
Sbjct: 883  AVDSYMTCAVRECYASFKNLINYLVVGEREGQVINDIFSKIDEHIEKETLITELNLSALP 942

Query: 183  SLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGKDG 242
             LY QFVRLIEY+L N+EEDKDQ+VIVLL+MLE+VTRDI+EEE+ SL+E++H GS+ K  
Sbjct: 943  DLYGQFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSYVKYD 1002

Query: 243  KPRSLD---RLFDKLNFPV-PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFS 302
                L    + F +L FPV  +TEAWKEKI+RLHLLLTVKESAMDVPS+LEARRR++FFS
Sbjct: 1003 VMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFS 1062

Query: 303  NSLFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWT 362
            NSLFM+MPPAPK++NMLSFS+LTPY+SEDVLFS+  LE+ NEDGVSILFYLQKIFP++WT
Sbjct: 1063 NSLFMDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFPDEWT 1122

Query: 363  NFLERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDTAEH 422
            NFLERV+CG+EEEL+  E+LEE+LRLWASYRGQTLTKTVRGMMY+RKALELQAFLD A+ 
Sbjct: 1123 NFLERVKCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKD 1182

Query: 423  QDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYK---------DAVDSPLWA 482
            +                                  ELLKGYK               LWA
Sbjct: 1183 E----------------------------------ELLKGYKALELTSEEASKSGGSLWA 1242

Query: 483  QCQAAVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSR 542
            QCQA  D+KF++VVSCQQY IHKRSGD RAKDILRLM  YPS+RVAYI+E+E+  K+  +
Sbjct: 1243 QCQALADMKFTFVVSCQQYSIHKRSGDQRAKDILRLMTTYPSIRVAYIDEVEQTHKESYK 1302

Query: 543  KTNQKSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFT 602
             T +K YYS L +AA  TK  DS E +Q+LDQ IYR+KLPGPAILGEGKPENQNHAIIFT
Sbjct: 1303 GTEEKIYYSALVKAAPQTKPMDSSESVQTLDQLIYRIKLPGPAILGEGKPENQNHAIIFT 1362

Query: 603  RGECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFM 662
            RGE LQTIDMNQDNYMEEAFKMRNLL+EFL  H GVR PTILGLREHIFTGSVSSLAWFM
Sbjct: 1363 RGEGLQTIDMNQDNYMEEAFKMRNLLQEFLEKHGGVRCPTILGLREHIFTGSVSSLAWFM 1408

Query: 663  SNQENSFVTIGQRLLANPLK 670
            SNQENSFVTIGQR+LA+PLK
Sbjct: 1423 SNQENSFVTIGQRVLASPLK 1408

BLAST of Lsi02G026150 vs. TAIR 10
Match: AT1G05570.2 (callose synthase 1 )

HSP 1 Score: 912.9 bits (2358), Expect = 1.5e-265
Identity = 466/680 (68.53%), Postives = 545/680 (80.15%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRF S+PGAFN CL+P + +D T+ K   A   RKFD++   +D +AA+FAQMWN+II S
Sbjct: 722  SRFQSIPGAFNDCLVPQDNSDDTKKKRFRATFSRKFDQLPSSKDKEAARFAQMWNKIISS 781

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDRELKKRL 122
            FREEDLI++REM+LLLVP W DP+L LI+WPPFLLASKIPIALDMAKDSNG+DRELKKRL
Sbjct: 782  FREEDLISDREMELLLVPYWSDPDLDLIRWPPFLLASKIPIALDMAKDSNGKDRELKKRL 841

Query: 123  TTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRALP 182
              D YM CAVRECYASFKN+IN+LV+GE E  VI +IF+ ID+HI++E LITEL++ ALP
Sbjct: 842  AVDSYMTCAVRECYASFKNLINYLVVGEREGQVINDIFSKIDEHIEKETLITELNLSALP 901

Query: 183  SLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGKDG 242
             LY QFVRLIEY+L N+EEDKDQ+VIVLL+MLE+VTRDI+EEE+ SL+E++H GS+ K  
Sbjct: 902  DLYGQFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSYVKYD 961

Query: 243  KPRSLD---RLFDKLNFPV-PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFS 302
                L    + F +L FPV  +TEAWKEKI+RLHLLLTVKESAMDVPS+LEARRR++FFS
Sbjct: 962  VMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFS 1021

Query: 303  NSLFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWT 362
            NSLFM+MPPAPK++NMLSFS+LTPY+SEDVLFS+  LE+ NEDGVSILFYLQKIFP++WT
Sbjct: 1022 NSLFMDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFPDEWT 1081

Query: 363  NFLERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDTAEH 422
            NFLERV+CG+EEEL+  E+LEE+LRLWASYRGQTLTKTVRGMMY+RKALELQAFLD A+ 
Sbjct: 1082 NFLERVKCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKD 1141

Query: 423  QDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYK---------DAVDSPLWA 482
            +                                  ELLKGYK               LWA
Sbjct: 1142 E----------------------------------ELLKGYKALELTSEEASKSGGSLWA 1201

Query: 483  QCQAAVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSR 542
            QCQA  D+KF++VVSCQQY IHKRSGD RAKDILRLM  YPS+RVAYI+E+E+  K+  +
Sbjct: 1202 QCQALADMKFTFVVSCQQYSIHKRSGDQRAKDILRLMTTYPSIRVAYIDEVEQTHKESYK 1261

Query: 543  KTNQKSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFT 602
             T +K YYS L +AA  TK  DS E +Q+LDQ IYR+KLPGPAILGEGKPENQNHAIIFT
Sbjct: 1262 GTEEKIYYSALVKAAPQTKPMDSSESVQTLDQLIYRIKLPGPAILGEGKPENQNHAIIFT 1321

Query: 603  RGECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFM 662
            RGE LQTIDMNQDNYMEEAFKMRNLL+EFL  H GVR PTILGLREHIFTGSVSSLAWFM
Sbjct: 1322 RGEGLQTIDMNQDNYMEEAFKMRNLLQEFLEKHGGVRCPTILGLREHIFTGSVSSLAWFM 1367

Query: 663  SNQENSFVTIGQRLLANPLK 670
            SNQENSFVTIGQR+LA+PLK
Sbjct: 1382 SNQENSFVTIGQRVLASPLK 1367

BLAST of Lsi02G026150 vs. TAIR 10
Match: AT2G31960.1 (glucan synthase-like 3 )

HSP 1 Score: 911.0 bits (2353), Expect = 5.7e-265
Identity = 469/680 (68.97%), Postives = 548/680 (80.59%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRF SLP AFNACL+P EK++  + KG+MA   RKFD++   +D +AA+FAQMWN+II S
Sbjct: 763  SRFQSLPEAFNACLVPNEKSETPKKKGIMATFTRKFDQVPSSKDKEAARFAQMWNKIISS 822

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDRELKKRL 122
            FREEDLI++REM+LLLVP W D +L LI+WPPFLLASKIPIALDMAKDSNG+DREL KRL
Sbjct: 823  FREEDLISDREMELLLVPYWADRDLDLIRWPPFLLASKIPIALDMAKDSNGKDRELTKRL 882

Query: 123  TTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRALP 182
            + D YM CAVRECYASFKN+INFLV+GE E  VI EIF+ ID+HI++E LI +L++ ALP
Sbjct: 883  SVDSYMTCAVRECYASFKNLINFLVVGEREGQVINEIFSRIDEHIEKETLIKDLNLSALP 942

Query: 183  SLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGKDG 242
             LY QFVRLIEY++ N+EEDKDQ+VIVLL+MLEVVTRDI++EE+ S++ES+H G++ K  
Sbjct: 943  DLYGQFVRLIEYLMENREEDKDQIVIVLLNMLEVVTRDIMDEEVPSMLESTHNGTYVKYD 1002

Query: 243  KPRSLD---RLFDKLNFPV-PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFS 302
                L    + F +L FPV  +TEAWKEKI+RLHLLLTVKESAMDVPS+LEARRR++FFS
Sbjct: 1003 VMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFS 1062

Query: 303  NSLFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWT 362
            NSLFMEMP APK++NMLSFS+LTPYYSEDVLFS+  LEK NEDGVSILFYLQKIFP++WT
Sbjct: 1063 NSLFMEMPDAPKIRNMLSFSVLTPYYSEDVLFSIFGLEKQNEDGVSILFYLQKIFPDEWT 1122

Query: 363  NFLERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDTAEH 422
            NFLERV+CGSEEEL+  EELEE+LRLWASYRGQTLTKTVRGMMY+RKALELQAFLD A+ 
Sbjct: 1123 NFLERVKCGSEEELRAREELEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKD 1182

Query: 423  QDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYK-------DAVDS--PLWA 482
            +                                  EL+KGYK       DA  S   LWA
Sbjct: 1183 E----------------------------------ELMKGYKALELTSEDASKSGTSLWA 1242

Query: 483  QCQAAVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSR 542
            QCQA  D+KF++VVSCQQY + KRSGD RAKDILRLM  YPSLRVAYI+E+E+  K+  +
Sbjct: 1243 QCQALADMKFTFVVSCQQYSVQKRSGDQRAKDILRLMTTYPSLRVAYIDEVEQTHKESYK 1302

Query: 543  KTNQKSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFT 602
              ++K YYS L +AA  TKS DS E +Q+LDQ IYR+KLPGPAILGEGKPENQNH+IIFT
Sbjct: 1303 GADEKIYYSALVKAAPQTKSMDSSESVQTLDQVIYRIKLPGPAILGEGKPENQNHSIIFT 1362

Query: 603  RGECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFM 662
            RGE LQTIDMNQDNYMEEAFKMRNLL+EFL  H GVR PTILGLREHIFTGSVSSLAWFM
Sbjct: 1363 RGEGLQTIDMNQDNYMEEAFKMRNLLQEFLVKHGGVRTPTILGLREHIFTGSVSSLAWFM 1408

Query: 663  SNQENSFVTIGQRLLANPLK 670
            SNQENSFVTIGQR+LA+PLK
Sbjct: 1423 SNQENSFVTIGQRVLASPLK 1408

BLAST of Lsi02G026150 vs. TAIR 10
Match: AT2G31960.2 (glucan synthase-like 3 )

HSP 1 Score: 911.0 bits (2353), Expect = 5.7e-265
Identity = 469/680 (68.97%), Postives = 548/680 (80.59%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKFDEITPDRDDQAAKFAQMWNEIIIS 62
            SRF SLP AFNACL+P EK++  + KG+MA   RKFD++   +D +AA+FAQMWN+II S
Sbjct: 763  SRFQSLPEAFNACLVPNEKSETPKKKGIMATFTRKFDQVPSSKDKEAARFAQMWNKIISS 822

Query: 63   FREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDRELKKRL 122
            FREEDLI++REM+LLLVP W D +L LI+WPPFLLASKIPIALDMAKDSNG+DREL KRL
Sbjct: 823  FREEDLISDREMELLLVPYWADRDLDLIRWPPFLLASKIPIALDMAKDSNGKDRELTKRL 882

Query: 123  TTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRALP 182
            + D YM CAVRECYASFKN+INFLV+GE E  VI EIF+ ID+HI++E LI +L++ ALP
Sbjct: 883  SVDSYMTCAVRECYASFKNLINFLVVGEREGQVINEIFSRIDEHIEKETLIKDLNLSALP 942

Query: 183  SLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEEISSLVESSHGGSFGKDG 242
             LY QFVRLIEY++ N+EEDKDQ+VIVLL+MLEVVTRDI++EE+ S++ES+H G++ K  
Sbjct: 943  DLYGQFVRLIEYLMENREEDKDQIVIVLLNMLEVVTRDIMDEEVPSMLESTHNGTYVKYD 1002

Query: 243  KPRSLD---RLFDKLNFPV-PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRRISFFS 302
                L    + F +L FPV  +TEAWKEKI+RLHLLLTVKESAMDVPS+LEARRR++FFS
Sbjct: 1003 VMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFS 1062

Query: 303  NSLFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIFPEQWT 362
            NSLFMEMP APK++NMLSFS+LTPYYSEDVLFS+  LEK NEDGVSILFYLQKIFP++WT
Sbjct: 1063 NSLFMEMPDAPKIRNMLSFSVLTPYYSEDVLFSIFGLEKQNEDGVSILFYLQKIFPDEWT 1122

Query: 363  NFLERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFLDTAEH 422
            NFLERV+CGSEEEL+  EELEE+LRLWASYRGQTLTKTVRGMMY+RKALELQAFLD A+ 
Sbjct: 1123 NFLERVKCGSEEELRAREELEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKD 1182

Query: 423  QDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYK-------DAVDS--PLWA 482
            +                                  EL+KGYK       DA  S   LWA
Sbjct: 1183 E----------------------------------ELMKGYKALELTSEDASKSGTSLWA 1242

Query: 483  QCQAAVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSR 542
            QCQA  D+KF++VVSCQQY + KRSGD RAKDILRLM  YPSLRVAYI+E+E+  K+  +
Sbjct: 1243 QCQALADMKFTFVVSCQQYSVQKRSGDQRAKDILRLMTTYPSLRVAYIDEVEQTHKESYK 1302

Query: 543  KTNQKSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFT 602
              ++K YYS L +AA  TKS DS E +Q+LDQ IYR+KLPGPAILGEGKPENQNH+IIFT
Sbjct: 1303 GADEKIYYSALVKAAPQTKSMDSSESVQTLDQVIYRIKLPGPAILGEGKPENQNHSIIFT 1362

Query: 603  RGECLQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFM 662
            RGE LQTIDMNQDNYMEEAFKMRNLL+EFL  H GVR PTILGLREHIFTGSVSSLAWFM
Sbjct: 1363 RGEGLQTIDMNQDNYMEEAFKMRNLLQEFLVKHGGVRTPTILGLREHIFTGSVSSLAWFM 1408

Query: 663  SNQENSFVTIGQRLLANPLK 670
            SNQENSFVTIGQR+LA+PLK
Sbjct: 1423 SNQENSFVTIGQRVLASPLK 1408

BLAST of Lsi02G026150 vs. TAIR 10
Match: AT5G13000.1 (glucan synthase-like 12 )

HSP 1 Score: 864.0 bits (2231), Expect = 7.9e-251
Identity = 460/676 (68.05%), Postives = 533/676 (78.85%), Query Frame = 0

Query: 3    SRFLSLPGAFNACLMPVEKTDHTRNKGLMANLGRKF--DEITPDRDDQAAKFAQMWNEII 62
            SRF SLPGAFN  L+P  K +  + KG+ A L   F  D++  +++ +AA+FAQ+WN II
Sbjct: 768  SRFESLPGAFNDRLIPDGK-NQQKKKGIRATLSHNFTEDKVPVNKEKEAARFAQLWNTII 827

Query: 63   ISFREEDLINNREMDLLLVPNWIDPELSLIQWPPFLLASKIPIALDMAKDSNGRDRELKK 122
             SFREEDLI++REMDLLLVP W D +L LIQWPPFLLASKIPIALDMAKDSNG+DRELKK
Sbjct: 828  SSFREEDLISDREMDLLLVPYWADRDLDLIQWPPFLLASKIPIALDMAKDSNGKDRELKK 887

Query: 123  RLTTDKYMLCAVRECYASFKNIINFLVLGEHEKLVIKEIFTIIDDHIKEENLITELDMRA 182
            R+ +D YM CAVRECYASFKNII F+V G  EK VI+ IF  +D HI   +LI E  M A
Sbjct: 888  RIESDTYMKCAVRECYASFKNIIKFVVQGNREKEVIEIIFAEVDKHIDTGDLIQEYKMSA 947

Query: 183  LPSLYDQFVRLIEYMLTNKEEDKDQVVIVLLDMLEVVTRDIIEEE--ISSLVESSHGGSF 242
            LPSLYD FV+LI+Y+L NKEED+D VVI+  DMLEVVTRDI+ E+  ISSLV+SSHGG++
Sbjct: 948  LPSLYDHFVKLIKYLLDNKEEDRDHVVILFQDMLEVVTRDIMMEDYNISSLVDSSHGGTW 1007

Query: 243  GKDGKPRSLD-RLF---DKLNFPV-PETEAWKEKIRRLHLLLTVKESAMDVPSDLEARRR 302
                 P     +LF     + FP+ P TEAWKEKI+R++LLLT KESAMDVPS+LEARRR
Sbjct: 1008 HGGMIPLEQQYQLFASSGAIRFPIEPVTEAWKEKIKRIYLLLTTKESAMDVPSNLEARRR 1067

Query: 303  ISFFSNSLFMEMPPAPKVQNMLSFSILTPYYSEDVLFSMNLLEKPNEDGVSILFYLQKIF 362
            ISFFSNSLFM+MP APKV+NMLSFS+LTPYY+E+VLFS+  LE PNEDGVSILFYLQKIF
Sbjct: 1068 ISFFSNSLFMDMPMAPKVRNMLSFSVLTPYYTEEVLFSLRDLETPNEDGVSILFYLQKIF 1127

Query: 363  PEQWTNFLERVQCGSEEELKNNEELEEQLRLWASYRGQTLTKTVRGMMYHRKALELQAFL 422
            P++W NFLERV+C SEEELK ++ELEE+LRLWASYRGQTLT+TVRGMMY+RKALELQAFL
Sbjct: 1128 PDEWNNFLERVKCLSEEELKESDELEEELRLWASYRGQTLTRTVRGMMYYRKALELQAFL 1187

Query: 423  DTAEHQDPYLLYLYEKKYVWRVRGRYKYKKSFYKFKCGYPELLKGYKDAVDSPLWAQCQA 482
            D A H+D  L+  Y+                         EL        +  LWAQCQA
Sbjct: 1188 DMAMHED--LMEGYKA-----------------------VELNSENNSRGERSLWAQCQA 1247

Query: 483  AVDLKFSYVVSCQQYGIHKRSGDARAKDILRLMAKYPSLRVAYIEELEEPSKDKSRKTNQ 542
              D+KF+YVVSCQQYGIHKRSGD RA+DILRLM +YPSLRVAYI+E+EEP KDKS+K NQ
Sbjct: 1248 VADMKFTYVVSCQQYGIHKRSGDPRAQDILRLMTRYPSLRVAYIDEVEEPVKDKSKKGNQ 1307

Query: 543  KSYYSVLARAALPTKSKDSIEPIQSLDQTIYRVKLPGPAILGEGKPENQNHAIIFTRGEC 602
            K YYSVL +     KS D     Q+LDQ IYR++LPGPAILGEGKPENQNHAIIF+RGE 
Sbjct: 1308 KVYYSVLVKV---PKSTDHSTLAQNLDQVIYRIRLPGPAILGEGKPENQNHAIIFSRGEG 1367

Query: 603  LQTIDMNQDNYMEEAFKMRNLLEEFLTMHDGVRYPTILGLREHIFTGSVSSLAWFMSNQE 662
            LQTIDMNQDNYMEEA KMRNLL+EFLT HDGVR+P+ILGLREHIFTGSVSSLAWFMSNQE
Sbjct: 1368 LQTIDMNQDNYMEEALKMRNLLQEFLTKHDGVRHPSILGLREHIFTGSVSSLAWFMSNQE 1414

Query: 663  NSFVTIGQRLLANPLK 670
             SFVTIGQRLLANPL+
Sbjct: 1428 TSFVTIGQRLLANPLR 1414