Lsi02G026140 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G026140
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description1,3-beta-glucan synthase
Locationchr02: 32580556 .. 32584462 (-)
RNA-Seq ExpressionLsi02G026140
SyntenyLsi02G026140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTTTTTAAGGGATTTTTATTTGTAGAACTTGATATTCTTTAAAGATGGAAAAAGGACTTATTTTATAGTTATTTGAGAAATTTTAGGCTCCATCACAAGCAGAATAACAAGAAGGATTCACTGTTATATCATATGTGATAATTAGCACATAGTATTAAGGTCTGAACTTCATTTTATTATTATCATTATAATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTGTTCTAAATTATACTAATAACTACCACATATTTTGGGTGCCTTAATGCTCAATTTAATGTATCTTATACCTTTGGCTTCTTATTCAGAGTTCGATTCCATTACGGCCATCCAGATGTTTTTGATAGATTGTTTCACCTTACCCGAGGGGGTGTTAGTAAAGCTTCCAAAGTCATCAACTTGAGTGAGGACATATTCGCAGGCATGTTCATAGTCATAAAAGTCCTTTGTTTTGTATTTAAACAAGTAATCTGGTTTAATCATATATTCTATTCTTGTATATGTGATTTTGAATTCCAGGTTTCAACTCTACATTGCGTGAAGGTAATGTTACTCATCATGAATACATACAAGTTGGTAAAGGGAGGGATGTTGGCCTCAACCAGATCTCAATGTTTGAGGCTAAGATAGCCAATGGTAATGGCGAACAGACATTAAGCCGTGATATGTACAGGCTAGGTCATCGATTTGATTTCTTTCGCATGTTGTCTTGTTATTTCACAACCATTGGTTTCTATTTCAGTACTATGGTAAGTATGAGAAGTATGAGAAGTTTGTCTTTATCGCATCTTTATTCATGAGATAAACTTAATTTTTCATTGACAGCTGACAGTGCTCACAGTCTATGTCTTTCTATATGGCCGCCTTTACCTTGTTCTCAGTGGCCTTGAAAAAGCTTTGAGTACTCACCCAGCCATTAAAGACAACAAGTCCCTTCAAACAGCTCTTATTTCACAGTCCGCTGTGCAAATTGGACTTCTGATGGCATTGCCTATGATGGTTGAAATAGGACTGGAAAGGGGATTTCGAGCAGCTTTAAGTGATTTCATACTAATGCAGCTGCAGCTTGCACCTGTGTTTTTCACTTTCTCTTTAGGAACCAAAACACATTATTATGGAAGGACATTGCTTCATGGGGGTGCGCAATACAGAGGTACCGGTCGTGGTTTTGTGGTGTTCCATGCCAAATTTGCTGACAATTATAGGATGTATTCTCGCAGTCATTTTGTCAAAGGAATTGAATTGATGATTCTTCTTCTGGTTTACCACATACTTGGCGTTTCATATAGAGGTGTGGTTGCTCATGTCTTGATCACCATATCAATATGGTTTATGGTAGGAACATGGCTTTTTGCACCTTTCCTCTTCAATCCATTAGGCTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAAAAATGGATTGTCAACCGTGGAGGAATCGGAGTATCTCCTGATAAAAGTTGGGAGTCTTGGTGGGAAAAAGAACATGAACATCTACATTACTCCGGAAAGCGTGGCATCTTCGTTGAGATATTATTGTCACTACGATTTTTCATCTTTCAATATGGACTAGTGTATCACTTGAAAATAATTGACTCCCAAAGTTTTCTGGTACGAATATTGTTCCCACATTCTTACCAATTCCTTGGTTTAACCGAGCATATAAGCATATTTATTCTACTTCAAATTTCAGGTTTATGGCCTTTCTTGGGTTGTGATCATTTCAATATTGCTCCTGATGAAGGTAAGAAATTTGTTATTCCGGATCCTCATCCCGTATTTGGGTTAAACCATAAACTTTGTGTTTAGAAATCTTATTTGGATTCATCTTGTTCACTGATGAATAAATAGTTGGGTCTCCAAGTAGGGAGACCTTTTATAACTGGGGTCATTTACATTATTCTAGAGAATAGAAACGTAAATGTTTCCTATGTGAGGATCCCATTACTACCCCTCTGCCTCTAGGCTTTATTCCTTTGTAATTATTCAATCCACCTTCTCTATTTCTTATTATATATATATATATATATATATATTTAAGATAGCCAGAATGGGACTTATCTATTGTTGCGCATAGATCTTTTTTTCCCAAGGGTTGAATGCGGAAAATTTCTCCAAAAAGGACATTGATGGGACAAAATAGTTTGCCATTTTCCATTTTATATGCAGAAGTTAGAAATATGTAACTTTTGCATGATTATTTTGTTGCTAATTAATTGAAGCAAATTTAGAGGCCTGGGATATTCCCACTCAAAGAATAATATGGAACCACTATTTTGTTGCTAGAAGTTTCCTTACTAGTTCTCCATTAACTCTATTTATCTGAAATGAACAATATGGAACTACTACTTTCCTGTTTCTGCTGCGATTATGTTAATACCATTTAAACTTTGTAGGCTGTATCCGTTGGACGACGTAAATTCAGTGCAAGCTTTCAGTTGTTATTTAGGCTGGCCGAGGGTTTTATTTTCATTATCTGCGTGGCTGGTTTCATAACTTTAGTTGCAATCCCTCATATGACAATCAGGGACATCATTCTTTGCATTCTTGCCTTCTTGCCAACAGGATGGGGCCTGCTTTTGGTGAGTTTACTGAAACATCTCCATGCTAGATACACTTTAGATGTTAGTGTTACATTCCTTTCTGCTCTCTAATTTATCAATTTTCTTCATCTCATGGTTCATCAATACAGATTGCCCAAGCTTGTAGGCCCTTAATTCATCATACTGCATTATGGGGATCTGTTCGCGCTCTGGCTCGGAGTTACGAAATTGTCATGGGGTTGCTTCTGTTCACACCTGTAGCATTTCTAGCCTGGTTTCCATTCGTTTCAGAGTTCCAGACACGAATGCTTTTTAACCAAGCATTCAGTAGAGGTCTGCAAATTTCTCGCATTCTTGGGGGGCCTAGCAAAAAGGAAAAGAGTTCCTTCACAGACAAAGAGTAAATATTCACAACCAACATACAAGATAAGATGACCACAGTTCTGAACTGTAGATATCTCTCAACTCTTTATTACTGTAAATACCGGTATATGCTTTGCTCTCTATATTTATGGGTTGCCTTTTCTCATTGAGATGATTATTAGACTGATGATGGTGGTCATTTGTTCAAATTACCATTTACCATCCGATCATATTTGTAGATTCATTATTTATAGTGAATACATTATGCATATATTTGATTTGAATTTGGTTTCAATTATAAGGATGAAGATTATGACAA

mRNA sequence

ATGGGAGTTCGATTCCATTACGGCCATCCAGATGTTTTTGATAGATTGTTTCACCTTACCCGAGGGGGTGTTAGTAAAGCTTCCAAAGTCATCAACTTGAGTTTCAACTCTACATTGCGTGAAGGTAATGTTACTCATCATGAATACATACAAGTTGGTAAAGGGAGGGATGTTGGCCTCAACCAGATCTCAATGTTTGAGGCTAAGATAGCCAATGGTAATGGCGAACAGACATTAAGCCGTGATATGTACAGGCTAGGTCATCGATTTGATTTCTTTCGCATGTTGTCTTGTTATTTCACAACCATTGGTTTCTATTTCAGTACTATGCTGACAGTGCTCACAGTCTATGTCTTTCTATATGGCCGCCTTTACCTTGTTCTCAGTGGCCTTGAAAAAGCTTTGAGTACTCACCCAGCCATTAAAGACAACAAGTCCCTTCAAACAGCTCTTATTTCACAGTCCGCTGTGCAAATTGGACTTCTGATGGCATTGCCTATGATGGTTGAAATAGGACTGGAAAGGGGATTTCGAGCAGCTTTAAGTGATTTCATACTAATGCAGCTGCAGCTTGCACCTGTGTTTTTCACTTTCTCTTTAGGAACCAAAACACATTATTATGGAAGGACATTGCTTCATGGGGGTGCGCAATACAGAGGTACCGGTCGTGGTTTTGTGGTGTTCCATGCCAAATTTGCTGACAATTATAGGATGTATTCTCGCAGTCATTTTGTCAAAGGAATTGAATTGATGATTCTTCTTCTGGTTTACCACATACTTGGCGTTTCATATAGAGGTGTGGTTGCTCATGTCTTGATCACCATATCAATATGGTTTATGGTAGGAACATGGCTTTTTGCACCTTTCCTCTTCAATCCATTAGGCTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAAAAATGGATTGTCAACCGTGGAGGAATCGGAGTATCTCCTGATAAAAGTTGGGAGTCTTGGTGGGAAAAAGAACATGAACATCTACATTACTCCGGAAAGCGTGGCATCTTCGTTGAGATATTATTGTCACTACGATTTTTCATCTTTCAATATGGACTAGTGTATCACTTGAAAATAATTGACTCCCAAAGTTTTCTGGTACGAATATTGTTCCCACATTCTTACCAATTCCTTGGTTTAACCGAGCATATAAGCATATTTATTCTACTTCAAATTTCAGGTTTATGGCCTTTCTTGGGTTGTGATCATTTCAATATTGCTCCTGATGAAGGTAAGAAATTTGTTATTCCGGATCCTCATCCCGCTGTATCCGTTGGACGACGTAAATTCAGTGCAAGCTTTCAGTTGTTATTTAGGCTGGCCGAGGGTTTTATTTTCATTATCTGCGTGGCTGGTTTCATAACTTTAGTTGCAATCCCTCATATGACAATCAGGGACATCATTCTTTGCATTCTTGCCTTCTTGCCAACAGGATGGGGCCTGCTTTTGGTGAGTTTACTGAAACATCTCCATGCTAGATACACTTTAGATATTGCCCAAGCTTGTAGGCCCTTAATTCATCATACTGCATTATGGGGATCTGTTCGCGCTCTGGCTCGGAGTTACGAAATTGTCATGGGGTTGCTTCTGTTCACACCTGTAGCATTTCTAGCCTGGTTTCCATTCGTTTCAGAGTTCCAGACACGAATGCTTTTTAACCAAGCATTCAGTAGAGGTCTGCAAATTTCTCGCATTCTTGGGGGGCCTAGCAAAAAGGAAAAGAGTTCCTTCACAGACAAAGAGTAAATATTCACAACCAACATACAAGATAAGATGACCACAGTTCTGAACTGTAGATATCTCTCAACTCTTTATTACTGTAAATACCGGTATATGCTTTGCTCTCTATATTTATGGGTTGCCTTTTCTCATTGAGATGATTATTAGACTGATGATGGTGGTCATTTGTTCAAATTACCATTTACCATCCGATCATATTTGTAGATTCATTATTTATAGTGAATACATTATGCATATATTTGATTTGAATTTGGTTTCAATTATAAGGATGAAGATTATGACAA

Coding sequence (CDS)

ATGGGAGTTCGATTCCATTACGGCCATCCAGATGTTTTTGATAGATTGTTTCACCTTACCCGAGGGGGTGTTAGTAAAGCTTCCAAAGTCATCAACTTGAGTTTCAACTCTACATTGCGTGAAGGTAATGTTACTCATCATGAATACATACAAGTTGGTAAAGGGAGGGATGTTGGCCTCAACCAGATCTCAATGTTTGAGGCTAAGATAGCCAATGGTAATGGCGAACAGACATTAAGCCGTGATATGTACAGGCTAGGTCATCGATTTGATTTCTTTCGCATGTTGTCTTGTTATTTCACAACCATTGGTTTCTATTTCAGTACTATGCTGACAGTGCTCACAGTCTATGTCTTTCTATATGGCCGCCTTTACCTTGTTCTCAGTGGCCTTGAAAAAGCTTTGAGTACTCACCCAGCCATTAAAGACAACAAGTCCCTTCAAACAGCTCTTATTTCACAGTCCGCTGTGCAAATTGGACTTCTGATGGCATTGCCTATGATGGTTGAAATAGGACTGGAAAGGGGATTTCGAGCAGCTTTAAGTGATTTCATACTAATGCAGCTGCAGCTTGCACCTGTGTTTTTCACTTTCTCTTTAGGAACCAAAACACATTATTATGGAAGGACATTGCTTCATGGGGGTGCGCAATACAGAGGTACCGGTCGTGGTTTTGTGGTGTTCCATGCCAAATTTGCTGACAATTATAGGATGTATTCTCGCAGTCATTTTGTCAAAGGAATTGAATTGATGATTCTTCTTCTGGTTTACCACATACTTGGCGTTTCATATAGAGGTGTGGTTGCTCATGTCTTGATCACCATATCAATATGGTTTATGGTAGGAACATGGCTTTTTGCACCTTTCCTCTTCAATCCATTAGGCTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAAAAATGGATTGTCAACCGTGGAGGAATCGGAGTATCTCCTGATAAAAGTTGGGAGTCTTGGTGGGAAAAAGAACATGAACATCTACATTACTCCGGAAAGCGTGGCATCTTCGTTGAGATATTATTGTCACTACGATTTTTCATCTTTCAATATGGACTAGTGTATCACTTGAAAATAATTGACTCCCAAAGTTTTCTGGTACGAATATTGTTCCCACATTCTTACCAATTCCTTGGTTTAACCGAGCATATAAGCATATTTATTCTACTTCAAATTTCAGGTTTATGGCCTTTCTTGGGTTGTGATCATTTCAATATTGCTCCTGATGAAGGTAAGAAATTTGTTATTCCGGATCCTCATCCCGCTGTATCCGTTGGACGACGTAAATTCAGTGCAAGCTTTCAGTTGTTATTTAGGCTGGCCGAGGGTTTTATTTTCATTATCTGCGTGGCTGGTTTCATAACTTTAGTTGCAATCCCTCATATGACAATCAGGGACATCATTCTTTGCATTCTTGCCTTCTTGCCAACAGGATGGGGCCTGCTTTTGGTGAGTTTACTGAAACATCTCCATGCTAGATACACTTTAGATATTGCCCAAGCTTGTAGGCCCTTAATTCATCATACTGCATTATGGGGATCTGTTCGCGCTCTGGCTCGGAGTTACGAAATTGTCATGGGGTTGCTTCTGTTCACACCTGTAGCATTTCTAGCCTGGTTTCCATTCGTTTCAGAGTTCCAGACACGAATGCTTTTTAACCAAGCATTCAGTAGAGGTCTGCAAATTTCTCGCATTCTTGGGGGGCCTAGCAAAAAGGAAAAGAGTTCCTTCACAGACAAAGAGTAA

Protein sequence

MGVRFHYGHPDVFDRLFHLTRGGVSKASKVINLSFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGFEWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIAPDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDIILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSSFTDKE
Homology
BLAST of Lsi02G026140 vs. ExPASy Swiss-Prot
Match: Q9LXT9 (Callose synthase 3 OS=Arabidopsis thaliana OX=3702 GN=CALS3 PE=3 SV=3)

HSP 1 Score: 820.5 bits (2118), Expect = 1.2e-236
Identity = 428/589 (72.67%), Postives = 477/589 (80.98%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1415 VRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1474

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRD+YRLGHRFDFFRM+SCYFTT+GFYFST++TVLTV
Sbjct: 1475 DVGLNQISMFEAKIANGNGEQTLSRDIYRLGHRFDFFRMMSCYFTTVGFYFSTLITVLTV 1534

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            Y+FLYGRLYLVLSGLE+ LST   I+DN  LQ AL SQS VQIG LMALPM++EIGLERG
Sbjct: 1535 YIFLYGRLYLVLSGLEQGLSTQKGIRDNTPLQIALASQSFVQIGFLMALPMLMEIGLERG 1594

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            FR ALS+F+LMQLQLAPVFFTFSLGTKTHYYGRTLLHGGA+YR TGRGFVVFHAKFADNY
Sbjct: 1595 FRTALSEFVLMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAKYRSTGRGFVVFHAKFADNY 1654

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R+YSRSHFVKG+E+M+LL+VY I G +YRGV+A++LITIS+WFMVGTWLFAPFLFNP GF
Sbjct: 1655 RLYSRSHFVKGLEMMLLLVVYQIFGSAYRGVLAYLLITISMWFMVGTWLFAPFLFNPSGF 1714

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDW KWI N GGIGV  +KSWESWWE+E EHL YSGKRGI VEILL+LRFF
Sbjct: 1715 EWQKIVDDWTDWNKWINNIGGIGVPAEKSWESWWEEEQEHLRYSGKRGIVVEILLALRFF 1774

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            I+QYGLVYHL I                     TE    F++  +S L  FL        
Sbjct: 1775 IYQYGLVYHLTI---------------------TEKTKNFLVYGVSWLVIFL-------- 1834

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                  FV+      VSVGRR+FSASFQL+FRL +G IF+  +A  + L+ + HMTI+DI
Sbjct: 1835 ----ILFVM----KTVSVGRRRFSASFQLMFRLIKGLIFMTFIAIIVILITLAHMTIQDI 1894

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
            I+CILAF+PTGWG+LL              IAQAC+P++H    WGSVR LAR YEIVMG
Sbjct: 1895 IVCILAFMPTGWGMLL--------------IAQACKPVVHRAGFWGSVRTLARGYEIVMG 1951

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSS 586
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG  +K++SS
Sbjct: 1955 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG-HRKDRSS 1951

BLAST of Lsi02G026140 vs. ExPASy Swiss-Prot
Match: Q9AUE0 (Callose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=CALS1 PE=1 SV=2)

HSP 1 Score: 807.0 bits (2083), Expect = 1.4e-232
Identity = 420/589 (71.31%), Postives = 467/589 (79.29%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPD+FDRLFHLTRGG+ KASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1409 VRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1468

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRD+YRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV
Sbjct: 1469 DVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 1528

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLE+ LS+  A ++NK L+ AL SQS VQIG LMALPMM+EIGLERG
Sbjct: 1529 YVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMALPMMMEIGLERG 1588

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            F  AL +F+LMQLQLA VFFTF LGTKTHYYGRTL HGGA+YRGTGRGFVVFHAKFA+NY
Sbjct: 1589 FHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENY 1648

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R YSRSHFVKGIELMILLLVY I G SYRGVV ++LIT+SIWFMV TWLFAPFLFNP GF
Sbjct: 1649 RFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGF 1708

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDW KWI NRGGIGV P+KSWESWWEKE EHL +SG RGI +EI L+LRFF
Sbjct: 1709 EWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRGITLEIFLALRFF 1768

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVYHL     ++         S+   G +  + +FILL + GL            
Sbjct: 1769 IFQYGLVYHLSTFKGKN--------QSFWVYGASWFVILFILLIVKGL------------ 1828

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                             VGRR+FS +FQLLFR+ +G +F+  VA  IT +A+P +TI+D+
Sbjct: 1829 ----------------GVGRRRFSTNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDL 1888

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
             +C+LAF+PTGWG+LL              IAQAC+PLI    +W SVR LAR YEIVMG
Sbjct: 1889 FICMLAFMPTGWGMLL--------------IAQACKPLIQQLGIWSSVRTLARGYEIVMG 1946

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSS 586
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG  +K++SS
Sbjct: 1949 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG-QRKDRSS 1946

BLAST of Lsi02G026140 vs. ExPASy Swiss-Prot
Match: Q9SL03 (Callose synthase 2 OS=Arabidopsis thaliana OX=3702 GN=CALS2 PE=2 SV=3)

HSP 1 Score: 796.2 bits (2055), Expect = 2.5e-229
Identity = 418/589 (70.97%), Postives = 460/589 (78.10%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGV KASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1409 VRFHYGHPDVFDRLFHLTRGGVCKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1468

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRD+YRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV
Sbjct: 1469 DVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 1528

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLE+ LS   A + N  LQ AL SQS VQIG LMALPMM+EIGLERG
Sbjct: 1529 YVFLYGRLYLVLSGLEEGLSNQKAFRSNMPLQAALASQSFVQIGFLMALPMMMEIGLERG 1588

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            F  AL DF+LMQLQLA VFFTF LGTKTHYYGRTL HGGA+YRGTGRGFVVFHAKFA+NY
Sbjct: 1589 FHNALIDFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENY 1648

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R YSRSHFVKGIELMILLLVY I G +YRGVV ++LIT+SIWFMV TWLFAPFLFNP GF
Sbjct: 1649 RFYSRSHFVKGIELMILLLVYQIFGHAYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGF 1708

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDW KWI NRGGIGV P+KSWESWWEKE  HL +SGKRGI +EI+L+LRFF
Sbjct: 1709 EWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKEIGHLRHSGKRGIILEIVLALRFF 1768

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVY L     ++         S    G +  + +FILL + GL            
Sbjct: 1769 IFQYGLVYQLSTFKQEN--------QSLWIYGASWFVILFILLIVKGL------------ 1828

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                             VGR++FS +FQLLFR+ +GF+F+  +   IT +A+  +T +DI
Sbjct: 1829 ----------------GVGRQRFSTNFQLLFRIIKGFVFLTFLGLLITFLALRFLTPKDI 1888

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
             LC+LAF+PTGWG+LL              IAQAC+PLI     W SVR LAR YEI+MG
Sbjct: 1889 FLCMLAFMPTGWGMLL--------------IAQACKPLIQRLGFWSSVRTLARGYEILMG 1946

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSS 586
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG  +K++SS
Sbjct: 1949 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG-QRKDRSS 1946

BLAST of Lsi02G026140 vs. ExPASy Swiss-Prot
Match: Q3B724 (Callose synthase 5 OS=Arabidopsis thaliana OX=3702 GN=CALS5 PE=1 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 2.2e-196
Identity = 359/586 (61.26%), Postives = 428/586 (73.04%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDR+FH+TRGG+SKAS+ INLS      FNSTLR GNVTHHEYIQVGKGR
Sbjct: 1384 VRFHYGHPDVFDRIFHITRGGISKASRGINLSEDIFAGFNSTLRRGNVTHHEYIQVGKGR 1443

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQIS+FEAK+A GNGEQTLSRD+YRLGHRFDFFRM+SCYFTT+GFY S+M+ VLTV
Sbjct: 1444 DVGLNQISLFEAKVACGNGEQTLSRDLYRLGHRFDFFRMMSCYFTTVGFYISSMIVVLTV 1503

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            Y FLYGRLYL LSG+E+A+    A K + SL+ A+ SQS VQ+GLLM LPM++EIGLERG
Sbjct: 1504 YAFLYGRLYLSLSGVEEAIVKFAAAKGDSSLKAAMASQSVVQLGLLMTLPMVMEIGLERG 1563

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            FR ALSD I+MQLQLAPVFFTFSLGTK HYYGRT+LHGG++YR TGRGFVV H KFA+NY
Sbjct: 1564 FRTALSDLIIMQLQLAPVFFTFSLGTKVHYYGRTILHGGSKYRATGRGFVVKHEKFAENY 1623

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            RMYSRSHFVKG+ELM+LL+ Y I G +    V + L+  S WF+VG+WLFAPF FNP GF
Sbjct: 1624 RMYSRSHFVKGMELMVLLICYRIYGKAAEDSVGYALVMGSTWFLVGSWLFAPFFFNPSGF 1683

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDW DW KWI +RGGIGV  +KSWESWWE+E EHL +SG  G F EI LSLR+F
Sbjct: 1684 EWQKIVDDWDDWNKWISSRGGIGVPANKSWESWWEEEQEHLLHSGFFGKFWEIFLSLRYF 1743

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            I+QYG+VY L +        R+   HS    GL+  + + +++ +               
Sbjct: 1744 IYQYGIVYQLNLTKES----RMGKQHSIIVYGLSWLVIVAVMIVLK-------------- 1803

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                           VS+GR+KFSA FQL+FRL + F+FI  V     L     +T+ DI
Sbjct: 1804 --------------IVSMGRKKFSADFQLMFRLLKLFLFIGSVVIVGMLFHFLKLTVGDI 1863

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
            +  +LAFLPTGW L              L I+Q  RPL+    +WGSV+ALAR YE +MG
Sbjct: 1864 MQSLLAFLPTGWAL--------------LQISQVARPLMKTVGMWGSVKALARGYEYIMG 1923

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKE 583
            +++F PV  LAWFPFVSEFQTR+LFNQAFSRGLQI RIL G  K++
Sbjct: 1924 VVIFMPVTVLAWFPFVSEFQTRLLFNQAFSRGLQIQRILAGGKKQK 1923

BLAST of Lsi02G026140 vs. ExPASy Swiss-Prot
Match: Q9LTG5 (Callose synthase 4 OS=Arabidopsis thaliana OX=3702 GN=CALS4 PE=3 SV=2)

HSP 1 Score: 684.5 bits (1765), Expect = 1.1e-195
Identity = 370/593 (62.39%), Postives = 425/593 (71.67%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDR+FHLTRGGVSKASKVINLS      FNSTLREG V+HHEYIQVGKGR
Sbjct: 1339 VRFHYGHPDVFDRVFHLTRGGVSKASKVINLSEDIFAGFNSTLREGTVSHHEYIQVGKGR 1398

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANG+GEQTLSRD+YRLGH+FDFFRMLSCYFTT+GFYF +MLTVLTV
Sbjct: 1399 DVGLNQISMFEAKIANGSGEQTLSRDLYRLGHQFDFFRMLSCYFTTVGFYFCSMLTVLTV 1458

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSG+EK L   P +     ++  L SQS VQI  LMA+PM++EIGLERG
Sbjct: 1459 YVFLYGRLYLVLSGVEKELGNKPMM-----MEIILASQSFVQIVFLMAMPMIMEIGLERG 1518

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            F  AL DF+LMQLQLA VFFTF LGTK HYY +TLLHGGA+YRGTGRGFVVFHAKFA+NY
Sbjct: 1519 FYDALFDFVLMQLQLASVFFTFQLGTKFHYYCKTLLHGGAEYRGTGRGFVVFHAKFAENY 1578

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R YSRSHFVK  EL ILLLVYHI G +Y G     L TISIWFMVGTWLFAPFLFNP GF
Sbjct: 1579 RFYSRSHFVKATELGILLLVYHIFGPTYIG-----LFTISIWFMVGTWLFAPFLFNPSGF 1638

Query: 303  EWQKIVDDWTDWKKWI-VNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRF 362
            EW +IV+DW DWKKWI  + GGIGV P+KSWESWWEK+ EHL +SGK GI VEI  +LRF
Sbjct: 1639 EWHEIVEDWADWKKWIEYDNGGIGVPPEKSWESWWEKDIEHLQHSGKWGIVVEIFFALRF 1698

Query: 363  FIFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNI 422
            FIFQYGLVY L    ++          S    G +  + + +LL ++             
Sbjct: 1699 FIFQYGLVYQLSAFKNKY--------SSLWVFGASWLLILILLLTVT------------- 1758

Query: 423  APDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRD 482
                            +   RR+    FQLLFR+ +  +F+  +A FITL+    +  +D
Sbjct: 1759 ---------------VLDYARRRLGTEFQLLFRIIKVSLFLAFMAIFITLMTCRLILPQD 1818

Query: 483  IILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVM 542
            + LC+LA +PTGWGLLL              IAQ+C+PLI    +W  V  LA  Y++VM
Sbjct: 1819 VFLCMLALIPTGWGLLL--------------IAQSCKPLIQQPGIWSWVMTLAWVYDLVM 1871

Query: 543  GLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSSFTD 589
            G LLF P+AF+AWFPF+SEFQTRMLFNQAFSRGL ISRIL G  K   S   D
Sbjct: 1879 GSLLFIPIAFMAWFPFISEFQTRMLFNQAFSRGLHISRILSGQRKHRSSKNKD 1871

BLAST of Lsi02G026140 vs. ExPASy TrEMBL
Match: A0A6J1JU28 (1,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111488369 PE=3 SV=1)

HSP 1 Score: 971.8 bits (2511), Expect = 1.2e-279
Identity = 511/594 (86.03%), Postives = 524/594 (88.22%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1394 VRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1453

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTV
Sbjct: 1454 DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTV 1513

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG
Sbjct: 1514 YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 1573

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHA+FADNY
Sbjct: 1574 FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHARFADNY 1633

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R+YSRSHFVKGIELMILLLVYHILGVSYRGVV HVLITISIWFMVGTWLFAPFLFNP GF
Sbjct: 1634 RLYSRSHFVKGIELMILLLVYHILGVSYRGVVVHVLITISIWFMVGTWLFAPFLFNPSGF 1693

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDWKKWIVNRGGIGVSPD+SWESWWEKEHEHL YSGKRGIFVEILLSLRFF
Sbjct: 1694 EWQKIVDDWTDWKKWIVNRGGIGVSPDRSWESWWEKEHEHLQYSGKRGIFVEILLSLRFF 1753

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVYHL+II+SQSFLV           GL+  + I ILL +               
Sbjct: 1754 IFQYGLVYHLEIINSQSFLV----------YGLSWVVIISILLLMK-------------- 1813

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                          AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVA+PHMTIRDI
Sbjct: 1814 --------------AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDI 1873

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
            ILC+LAFLPTGWGLLL              IAQACRPLIH+TA+WGSVRALARSYEIVMG
Sbjct: 1874 ILCLLAFLPTGWGLLL--------------IAQACRPLIHNTAIWGSVRALARSYEIVMG 1933

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSSFTDKE 591
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGP KKEKSS TDKE
Sbjct: 1934 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1935

BLAST of Lsi02G026140 vs. ExPASy TrEMBL
Match: A0A6J1GN91 (1,3-beta-glucan synthase OS=Cucurbita moschata OX=3662 GN=LOC111455968 PE=3 SV=1)

HSP 1 Score: 971.8 bits (2511), Expect = 1.2e-279
Identity = 511/594 (86.03%), Postives = 524/594 (88.22%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1394 VRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1453

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTV
Sbjct: 1454 DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTV 1513

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG
Sbjct: 1514 YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 1573

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHA+FADNY
Sbjct: 1574 FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHARFADNY 1633

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R+YSRSHFVKGIELMILLLVYHILGVSYRGVV HVLITISIWFMVGTWLFAPFLFNP GF
Sbjct: 1634 RLYSRSHFVKGIELMILLLVYHILGVSYRGVVVHVLITISIWFMVGTWLFAPFLFNPSGF 1693

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDWKKWIVNRGGIGVSPD+SWESWWEKEHEHL YSGKRGIFVEILLSLRFF
Sbjct: 1694 EWQKIVDDWTDWKKWIVNRGGIGVSPDRSWESWWEKEHEHLQYSGKRGIFVEILLSLRFF 1753

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVYHL+II+SQSFLV           GL+  + I ILL +               
Sbjct: 1754 IFQYGLVYHLEIINSQSFLV----------YGLSWVVIISILLLMK-------------- 1813

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                          AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVA+PHMTIRDI
Sbjct: 1814 --------------AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDI 1873

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
            ILC+LAFLPTGWGLLL              IAQACRPLIH+TA+WGSVRALARSYEIVMG
Sbjct: 1874 ILCLLAFLPTGWGLLL--------------IAQACRPLIHNTAIWGSVRALARSYEIVMG 1933

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSSFTDKE 591
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGP KKEKSS TDKE
Sbjct: 1934 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1935

BLAST of Lsi02G026140 vs. ExPASy TrEMBL
Match: A0A0A0K8F7 (1,3-beta-glucan synthase OS=Cucumis sativus OX=3659 GN=Csa_7G429550 PE=3 SV=1)

HSP 1 Score: 969.1 bits (2504), Expect = 8.0e-279
Identity = 510/594 (85.86%), Postives = 523/594 (88.05%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1406 VRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1465

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTV
Sbjct: 1466 DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTV 1525

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLE+ALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG
Sbjct: 1526 YVFLYGRLYLVLSGLERALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 1585

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGA+YRGTGRGFVVFHAKFA+NY
Sbjct: 1586 FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAEYRGTGRGFVVFHAKFAENY 1645

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNP GF
Sbjct: 1646 RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGF 1705

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEH+HL +SGKRGIFVEILLS+RFF
Sbjct: 1706 EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHDHLKFSGKRGIFVEILLSIRFF 1765

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVYHLKII+SQSFLV           GL+  + I ILL +               
Sbjct: 1766 IFQYGLVYHLKIIESQSFLV----------YGLSWVVIISILLLMK-------------- 1825

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                          AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI
Sbjct: 1826 --------------AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 1885

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
            ILCILAFLPTGWGLLL              IAQAC+PLIH T LWGSVRALARSYEIVMG
Sbjct: 1886 ILCILAFLPTGWGLLL--------------IAQACKPLIHQTPLWGSVRALARSYEIVMG 1945

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSSFTDKE 591
            LLLFTP+AFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGP KKEKSS TDKE
Sbjct: 1946 LLLFTPIAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSTTDKE 1947

BLAST of Lsi02G026140 vs. ExPASy TrEMBL
Match: A0A5A7UU13 (1,3-beta-glucan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001200 PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.3e-278
Identity = 511/603 (84.74%), Postives = 523/603 (86.73%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS---------------FNSTLREGNVTHH 62
            VRFHYGHPDVFDRLFHLTRGG+SKASKVINLS               FNSTLREGNVTHH
Sbjct: 1433 VRFHYGHPDVFDRLFHLTRGGISKASKVINLSEDIFAGMLIVVKALCFNSTLREGNVTHH 1492

Query: 63   EYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYF 122
            EYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTT+GFYF
Sbjct: 1493 EYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTVGFYF 1552

Query: 123  STMLTVLTVYVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPM 182
            STMLTVLTVYVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPM
Sbjct: 1553 STMLTVLTVYVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPM 1612

Query: 183  MVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVV 242
            MVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGA+YRGTGRGFVV
Sbjct: 1613 MVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAEYRGTGRGFVV 1672

Query: 243  FHAKFADNYRMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFA 302
            FHAKFA+NYRMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFA
Sbjct: 1673 FHAKFAENYRMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFA 1732

Query: 303  PFLFNPLGFEWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFV 362
            PFLFNP GFEWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHL +SGKRGIFV
Sbjct: 1733 PFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLKFSGKRGIFV 1792

Query: 363  EILLSLRFFIFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPF 422
            EILLS+RFFIFQYGLVYHLKII+SQSFLV           GL+  + I ILL +      
Sbjct: 1793 EILLSIRFFIFQYGLVYHLKIIESQSFLV----------YGLSWVVIISILLLMK----- 1852

Query: 423  LGCDHFNIAPDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVA 482
                                   AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVA
Sbjct: 1853 -----------------------AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVA 1912

Query: 483  IPHMTIRDIILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRAL 542
            IPHMTIRDIILCILAFLPTGWGLLL              IAQAC+PLIH T LWGSVRAL
Sbjct: 1913 IPHMTIRDIILCILAFLPTGWGLLL--------------IAQACKPLIHQTPLWGSVRAL 1972

Query: 543  ARSYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSSFT 591
            ARSYEIVMGLLLFTP+AFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGP KKEKSS T
Sbjct: 1973 ARSYEIVMGLLLFTPIAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSTT 1983

BLAST of Lsi02G026140 vs. ExPASy TrEMBL
Match: A0A1S3CEN5 (1,3-beta-glucan synthase OS=Cucumis melo OX=3656 GN=LOC103499883 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.1e-275
Identity = 502/587 (85.52%), Postives = 517/587 (88.07%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGG+SKASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1406 VRFHYGHPDVFDRLFHLTRGGISKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1465

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTV
Sbjct: 1466 DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTV 1525

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG
Sbjct: 1526 YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 1585

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGA+YRGTGRGFVVFHAKFA+NY
Sbjct: 1586 FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAEYRGTGRGFVVFHAKFAENY 1645

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNP GF
Sbjct: 1646 RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGF 1705

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHL +SGKRGIFVEILLS+RFF
Sbjct: 1706 EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLKFSGKRGIFVEILLSIRFF 1765

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVYHLKII+SQSFLV           GL+  + I ILL +               
Sbjct: 1766 IFQYGLVYHLKIIESQSFLV----------YGLSWVVIISILLLMK-------------- 1825

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                          AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI
Sbjct: 1826 --------------AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 1885

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
            ILCILAFLPTGWGLLL              IAQAC+PLIH T LWGSVRALARSYEIVMG
Sbjct: 1886 ILCILAFLPTGWGLLL--------------IAQACKPLIHQTPLWGSVRALARSYEIVMG 1940

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEK 584
            LLLFTP+AFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGP K+++
Sbjct: 1946 LLLFTPIAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPRKRKR 1940

BLAST of Lsi02G026140 vs. NCBI nr
Match: XP_038898164.1 (callose synthase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 977.6 bits (2526), Expect = 4.7e-281
Identity = 515/594 (86.70%), Postives = 524/594 (88.22%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1114 VRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1173

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTT+GFYFST+LTVLTV
Sbjct: 1174 DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTVGFYFSTLLTVLTV 1233

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG
Sbjct: 1234 YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 1293

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGA+YRGTGRGFVVFHAKFADNY
Sbjct: 1294 FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAEYRGTGRGFVVFHAKFADNY 1353

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNP GF
Sbjct: 1354 RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGF 1413

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEH+HL YSGKRGIF EILLSLRFF
Sbjct: 1414 EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHDHLKYSGKRGIFAEILLSLRFF 1473

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVYHLKIIDSQSFLV           GL+  + I ILL +               
Sbjct: 1474 IFQYGLVYHLKIIDSQSFLV----------YGLSWVVIISILLLMK-------------- 1533

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                          AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI
Sbjct: 1534 --------------AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 1593

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
            ILC+LAFLPTGWGLLL              IAQAC+PLIHHTALWGSVRALARSYEIVMG
Sbjct: 1594 ILCLLAFLPTGWGLLL--------------IAQACKPLIHHTALWGSVRALARSYEIVMG 1653

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSSFTDKE 591
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGP KKEKSS TDKE
Sbjct: 1654 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1655

BLAST of Lsi02G026140 vs. NCBI nr
Match: XP_038898161.1 (callose synthase 1-like isoform X1 [Benincasa hispida] >XP_038898162.1 callose synthase 1-like isoform X1 [Benincasa hispida] >XP_038898163.1 callose synthase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 977.6 bits (2526), Expect = 4.7e-281
Identity = 515/594 (86.70%), Postives = 524/594 (88.22%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1396 VRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1455

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTT+GFYFST+LTVLTV
Sbjct: 1456 DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTVGFYFSTLLTVLTV 1515

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG
Sbjct: 1516 YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 1575

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGA+YRGTGRGFVVFHAKFADNY
Sbjct: 1576 FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAEYRGTGRGFVVFHAKFADNY 1635

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNP GF
Sbjct: 1636 RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGF 1695

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEH+HL YSGKRGIF EILLSLRFF
Sbjct: 1696 EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHDHLKYSGKRGIFAEILLSLRFF 1755

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVYHLKIIDSQSFLV           GL+  + I ILL +               
Sbjct: 1756 IFQYGLVYHLKIIDSQSFLV----------YGLSWVVIISILLLMK-------------- 1815

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                          AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI
Sbjct: 1816 --------------AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 1875

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
            ILC+LAFLPTGWGLLL              IAQAC+PLIHHTALWGSVRALARSYEIVMG
Sbjct: 1876 ILCLLAFLPTGWGLLL--------------IAQACKPLIHHTALWGSVRALARSYEIVMG 1935

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSSFTDKE 591
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGP KKEKSS TDKE
Sbjct: 1936 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1937

BLAST of Lsi02G026140 vs. NCBI nr
Match: XP_022953403.1 (callose synthase 1 [Cucurbita moschata] >XP_022953404.1 callose synthase 1 [Cucurbita moschata] >XP_022953405.1 callose synthase 1 [Cucurbita moschata])

HSP 1 Score: 971.8 bits (2511), Expect = 2.6e-279
Identity = 511/594 (86.03%), Postives = 524/594 (88.22%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1394 VRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1453

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTV
Sbjct: 1454 DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTV 1513

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG
Sbjct: 1514 YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 1573

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHA+FADNY
Sbjct: 1574 FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHARFADNY 1633

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R+YSRSHFVKGIELMILLLVYHILGVSYRGVV HVLITISIWFMVGTWLFAPFLFNP GF
Sbjct: 1634 RLYSRSHFVKGIELMILLLVYHILGVSYRGVVVHVLITISIWFMVGTWLFAPFLFNPSGF 1693

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDWKKWIVNRGGIGVSPD+SWESWWEKEHEHL YSGKRGIFVEILLSLRFF
Sbjct: 1694 EWQKIVDDWTDWKKWIVNRGGIGVSPDRSWESWWEKEHEHLQYSGKRGIFVEILLSLRFF 1753

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVYHL+II+SQSFLV           GL+  + I ILL +               
Sbjct: 1754 IFQYGLVYHLEIINSQSFLV----------YGLSWVVIISILLLMK-------------- 1813

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                          AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVA+PHMTIRDI
Sbjct: 1814 --------------AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDI 1873

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
            ILC+LAFLPTGWGLLL              IAQACRPLIH+TA+WGSVRALARSYEIVMG
Sbjct: 1874 ILCLLAFLPTGWGLLL--------------IAQACRPLIHNTAIWGSVRALARSYEIVMG 1933

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSSFTDKE 591
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGP KKEKSS TDKE
Sbjct: 1934 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1935

BLAST of Lsi02G026140 vs. NCBI nr
Match: KAG6575662.1 (Callose synthase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 971.8 bits (2511), Expect = 2.6e-279
Identity = 511/594 (86.03%), Postives = 524/594 (88.22%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1424 VRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1483

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTV
Sbjct: 1484 DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTV 1543

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG
Sbjct: 1544 YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 1603

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHA+FADNY
Sbjct: 1604 FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHARFADNY 1663

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R+YSRSHFVKGIELMILLLVYHILGVSYRGVV HVLITISIWFMVGTWLFAPFLFNP GF
Sbjct: 1664 RLYSRSHFVKGIELMILLLVYHILGVSYRGVVVHVLITISIWFMVGTWLFAPFLFNPSGF 1723

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDWKKWIVNRGGIGVSPD+SWESWWEKEHEHL YSGKRGIFVEILLSLRFF
Sbjct: 1724 EWQKIVDDWTDWKKWIVNRGGIGVSPDRSWESWWEKEHEHLQYSGKRGIFVEILLSLRFF 1783

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVYHL+II+SQSFLV           GL+  + I ILL +               
Sbjct: 1784 IFQYGLVYHLEIINSQSFLV----------YGLSWVVIISILLLMK-------------- 1843

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                          AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVA+PHMTIRDI
Sbjct: 1844 --------------AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDI 1903

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
            ILC+LAFLPTGWGLLL              IAQACRPLIH+TA+WGSVRALARSYEIVMG
Sbjct: 1904 ILCLLAFLPTGWGLLL--------------IAQACRPLIHNTAIWGSVRALARSYEIVMG 1963

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSSFTDKE 591
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGP KKEKSS TDKE
Sbjct: 1964 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1965

BLAST of Lsi02G026140 vs. NCBI nr
Match: XP_022991842.1 (callose synthase 1 [Cucurbita maxima] >XP_022991843.1 callose synthase 1 [Cucurbita maxima] >XP_022991845.1 callose synthase 1 [Cucurbita maxima])

HSP 1 Score: 971.8 bits (2511), Expect = 2.6e-279
Identity = 511/594 (86.03%), Postives = 524/594 (88.22%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1394 VRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1453

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTV
Sbjct: 1454 DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTV 1513

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG
Sbjct: 1514 YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 1573

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHA+FADNY
Sbjct: 1574 FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHARFADNY 1633

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R+YSRSHFVKGIELMILLLVYHILGVSYRGVV HVLITISIWFMVGTWLFAPFLFNP GF
Sbjct: 1634 RLYSRSHFVKGIELMILLLVYHILGVSYRGVVVHVLITISIWFMVGTWLFAPFLFNPSGF 1693

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDWKKWIVNRGGIGVSPD+SWESWWEKEHEHL YSGKRGIFVEILLSLRFF
Sbjct: 1694 EWQKIVDDWTDWKKWIVNRGGIGVSPDRSWESWWEKEHEHLQYSGKRGIFVEILLSLRFF 1753

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVYHL+II+SQSFLV           GL+  + I ILL +               
Sbjct: 1754 IFQYGLVYHLEIINSQSFLV----------YGLSWVVIISILLLMK-------------- 1813

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                          AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVA+PHMTIRDI
Sbjct: 1814 --------------AVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDI 1873

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
            ILC+LAFLPTGWGLLL              IAQACRPLIH+TA+WGSVRALARSYEIVMG
Sbjct: 1874 ILCLLAFLPTGWGLLL--------------IAQACRPLIHNTAIWGSVRALARSYEIVMG 1933

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSSFTDKE 591
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGP KKEKSS TDKE
Sbjct: 1934 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1935

BLAST of Lsi02G026140 vs. TAIR 10
Match: AT5G13000.1 (glucan synthase-like 12 )

HSP 1 Score: 820.5 bits (2118), Expect = 8.9e-238
Identity = 428/589 (72.67%), Postives = 477/589 (80.98%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1415 VRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1474

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRD+YRLGHRFDFFRM+SCYFTT+GFYFST++TVLTV
Sbjct: 1475 DVGLNQISMFEAKIANGNGEQTLSRDIYRLGHRFDFFRMMSCYFTTVGFYFSTLITVLTV 1534

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            Y+FLYGRLYLVLSGLE+ LST   I+DN  LQ AL SQS VQIG LMALPM++EIGLERG
Sbjct: 1535 YIFLYGRLYLVLSGLEQGLSTQKGIRDNTPLQIALASQSFVQIGFLMALPMLMEIGLERG 1594

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            FR ALS+F+LMQLQLAPVFFTFSLGTKTHYYGRTLLHGGA+YR TGRGFVVFHAKFADNY
Sbjct: 1595 FRTALSEFVLMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAKYRSTGRGFVVFHAKFADNY 1654

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R+YSRSHFVKG+E+M+LL+VY I G +YRGV+A++LITIS+WFMVGTWLFAPFLFNP GF
Sbjct: 1655 RLYSRSHFVKGLEMMLLLVVYQIFGSAYRGVLAYLLITISMWFMVGTWLFAPFLFNPSGF 1714

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDW KWI N GGIGV  +KSWESWWE+E EHL YSGKRGI VEILL+LRFF
Sbjct: 1715 EWQKIVDDWTDWNKWINNIGGIGVPAEKSWESWWEEEQEHLRYSGKRGIVVEILLALRFF 1774

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            I+QYGLVYHL I                     TE    F++  +S L  FL        
Sbjct: 1775 IYQYGLVYHLTI---------------------TEKTKNFLVYGVSWLVIFL-------- 1834

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                  FV+      VSVGRR+FSASFQL+FRL +G IF+  +A  + L+ + HMTI+DI
Sbjct: 1835 ----ILFVM----KTVSVGRRRFSASFQLMFRLIKGLIFMTFIAIIVILITLAHMTIQDI 1894

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
            I+CILAF+PTGWG+LL              IAQAC+P++H    WGSVR LAR YEIVMG
Sbjct: 1895 IVCILAFMPTGWGMLL--------------IAQACKPVVHRAGFWGSVRTLARGYEIVMG 1951

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSS 586
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG  +K++SS
Sbjct: 1955 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG-HRKDRSS 1951

BLAST of Lsi02G026140 vs. TAIR 10
Match: AT1G05570.1 (callose synthase 1 )

HSP 1 Score: 807.0 bits (2083), Expect = 1.0e-233
Identity = 420/589 (71.31%), Postives = 467/589 (79.29%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPD+FDRLFHLTRGG+ KASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1409 VRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1468

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRD+YRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV
Sbjct: 1469 DVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 1528

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLE+ LS+  A ++NK L+ AL SQS VQIG LMALPMM+EIGLERG
Sbjct: 1529 YVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMALPMMMEIGLERG 1588

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            F  AL +F+LMQLQLA VFFTF LGTKTHYYGRTL HGGA+YRGTGRGFVVFHAKFA+NY
Sbjct: 1589 FHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENY 1648

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R YSRSHFVKGIELMILLLVY I G SYRGVV ++LIT+SIWFMV TWLFAPFLFNP GF
Sbjct: 1649 RFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGF 1708

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDW KWI NRGGIGV P+KSWESWWEKE EHL +SG RGI +EI L+LRFF
Sbjct: 1709 EWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRGITLEIFLALRFF 1768

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVYHL     ++         S+   G +  + +FILL + GL            
Sbjct: 1769 IFQYGLVYHLSTFKGKN--------QSFWVYGASWFVILFILLIVKGL------------ 1828

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                             VGRR+FS +FQLLFR+ +G +F+  VA  IT +A+P +TI+D+
Sbjct: 1829 ----------------GVGRRRFSTNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDL 1888

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
             +C+LAF+PTGWG+LL              IAQAC+PLI    +W SVR LAR YEIVMG
Sbjct: 1889 FICMLAFMPTGWGMLL--------------IAQACKPLIQQLGIWSSVRTLARGYEIVMG 1946

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSS 586
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG  +K++SS
Sbjct: 1949 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG-QRKDRSS 1946

BLAST of Lsi02G026140 vs. TAIR 10
Match: AT1G05570.2 (callose synthase 1 )

HSP 1 Score: 807.0 bits (2083), Expect = 1.0e-233
Identity = 420/589 (71.31%), Postives = 467/589 (79.29%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPD+FDRLFHLTRGG+ KASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1368 VRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1427

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRD+YRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV
Sbjct: 1428 DVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 1487

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLE+ LS+  A ++NK L+ AL SQS VQIG LMALPMM+EIGLERG
Sbjct: 1488 YVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMALPMMMEIGLERG 1547

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            F  AL +F+LMQLQLA VFFTF LGTKTHYYGRTL HGGA+YRGTGRGFVVFHAKFA+NY
Sbjct: 1548 FHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENY 1607

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R YSRSHFVKGIELMILLLVY I G SYRGVV ++LIT+SIWFMV TWLFAPFLFNP GF
Sbjct: 1608 RFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGF 1667

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDW KWI NRGGIGV P+KSWESWWEKE EHL +SG RGI +EI L+LRFF
Sbjct: 1668 EWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRGITLEIFLALRFF 1727

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVYHL     ++         S+   G +  + +FILL + GL            
Sbjct: 1728 IFQYGLVYHLSTFKGKN--------QSFWVYGASWFVILFILLIVKGL------------ 1787

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                             VGRR+FS +FQLLFR+ +G +F+  VA  IT +A+P +TI+D+
Sbjct: 1788 ----------------GVGRRRFSTNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDL 1847

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
             +C+LAF+PTGWG+LL              IAQAC+PLI    +W SVR LAR YEIVMG
Sbjct: 1848 FICMLAFMPTGWGMLL--------------IAQACKPLIQQLGIWSSVRTLARGYEIVMG 1905

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSS 586
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG  +K++SS
Sbjct: 1908 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG-QRKDRSS 1905

BLAST of Lsi02G026140 vs. TAIR 10
Match: AT2G31960.1 (glucan synthase-like 3 )

HSP 1 Score: 796.2 bits (2055), Expect = 1.8e-230
Identity = 418/589 (70.97%), Postives = 460/589 (78.10%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGV KASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1409 VRFHYGHPDVFDRLFHLTRGGVCKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1468

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRD+YRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV
Sbjct: 1469 DVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 1528

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLE+ LS   A + N  LQ AL SQS VQIG LMALPMM+EIGLERG
Sbjct: 1529 YVFLYGRLYLVLSGLEEGLSNQKAFRSNMPLQAALASQSFVQIGFLMALPMMMEIGLERG 1588

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            F  AL DF+LMQLQLA VFFTF LGTKTHYYGRTL HGGA+YRGTGRGFVVFHAKFA+NY
Sbjct: 1589 FHNALIDFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENY 1648

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R YSRSHFVKGIELMILLLVY I G +YRGVV ++LIT+SIWFMV TWLFAPFLFNP GF
Sbjct: 1649 RFYSRSHFVKGIELMILLLVYQIFGHAYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGF 1708

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDW KWI NRGGIGV P+KSWESWWEKE  HL +SGKRGI +EI+L+LRFF
Sbjct: 1709 EWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKEIGHLRHSGKRGIILEIVLALRFF 1768

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVY L     ++         S    G +  + +FILL + GL            
Sbjct: 1769 IFQYGLVYQLSTFKQEN--------QSLWIYGASWFVILFILLIVKGL------------ 1828

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                             VGR++FS +FQLLFR+ +GF+F+  +   IT +A+  +T +DI
Sbjct: 1829 ----------------GVGRQRFSTNFQLLFRIIKGFVFLTFLGLLITFLALRFLTPKDI 1888

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
             LC+LAF+PTGWG+LL              IAQAC+PLI     W SVR LAR YEI+MG
Sbjct: 1889 FLCMLAFMPTGWGMLL--------------IAQACKPLIQRLGFWSSVRTLARGYEILMG 1946

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSS 586
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG  +K++SS
Sbjct: 1949 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG-QRKDRSS 1946

BLAST of Lsi02G026140 vs. TAIR 10
Match: AT2G31960.2 (glucan synthase-like 3 )

HSP 1 Score: 796.2 bits (2055), Expect = 1.8e-230
Identity = 418/589 (70.97%), Postives = 460/589 (78.10%), Query Frame = 0

Query: 3    VRFHYGHPDVFDRLFHLTRGGVSKASKVINLS------FNSTLREGNVTHHEYIQVGKGR 62
            VRFHYGHPDVFDRLFHLTRGGV KASKVINLS      FNSTLREGNVTHHEYIQVGKGR
Sbjct: 1409 VRFHYGHPDVFDRLFHLTRGGVCKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1468

Query: 63   DVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 122
            DVGLNQISMFEAKIANGNGEQTLSRD+YRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV
Sbjct: 1469 DVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 1528

Query: 123  YVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERG 182
            YVFLYGRLYLVLSGLE+ LS   A + N  LQ AL SQS VQIG LMALPMM+EIGLERG
Sbjct: 1529 YVFLYGRLYLVLSGLEEGLSNQKAFRSNMPLQAALASQSFVQIGFLMALPMMMEIGLERG 1588

Query: 183  FRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNY 242
            F  AL DF+LMQLQLA VFFTF LGTKTHYYGRTL HGGA+YRGTGRGFVVFHAKFA+NY
Sbjct: 1589 FHNALIDFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENY 1648

Query: 243  RMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPLGF 302
            R YSRSHFVKGIELMILLLVY I G +YRGVV ++LIT+SIWFMV TWLFAPFLFNP GF
Sbjct: 1649 RFYSRSHFVKGIELMILLLVYQIFGHAYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGF 1708

Query: 303  EWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLHYSGKRGIFVEILLSLRFF 362
            EWQKIVDDWTDW KWI NRGGIGV P+KSWESWWEKE  HL +SGKRGI +EI+L+LRFF
Sbjct: 1709 EWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKEIGHLRHSGKRGIILEIVLALRFF 1768

Query: 363  IFQYGLVYHLKIIDSQSFLVRILFPHSYQFLGLTEHISIFILLQISGLWPFLGCDHFNIA 422
            IFQYGLVY L     ++         S    G +  + +FILL + GL            
Sbjct: 1769 IFQYGLVYQLSTFKQEN--------QSLWIYGASWFVILFILLIVKGL------------ 1828

Query: 423  PDEGKKFVIPDPHPAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDI 482
                             VGR++FS +FQLLFR+ +GF+F+  +   IT +A+  +T +DI
Sbjct: 1829 ----------------GVGRQRFSTNFQLLFRIIKGFVFLTFLGLLITFLALRFLTPKDI 1888

Query: 483  ILCILAFLPTGWGLLLVSLLKHLHARYTLDIAQACRPLIHHTALWGSVRALARSYEIVMG 542
             LC+LAF+PTGWG+LL              IAQAC+PLI     W SVR LAR YEI+MG
Sbjct: 1889 FLCMLAFMPTGWGMLL--------------IAQACKPLIQRLGFWSSVRTLARGYEILMG 1946

Query: 543  LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPSKKEKSS 586
            LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG  +K++SS
Sbjct: 1949 LLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG-QRKDRSS 1946

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LXT91.2e-23672.67Callose synthase 3 OS=Arabidopsis thaliana OX=3702 GN=CALS3 PE=3 SV=3[more]
Q9AUE01.4e-23271.31Callose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=CALS1 PE=1 SV=2[more]
Q9SL032.5e-22970.97Callose synthase 2 OS=Arabidopsis thaliana OX=3702 GN=CALS2 PE=2 SV=3[more]
Q3B7242.2e-19661.26Callose synthase 5 OS=Arabidopsis thaliana OX=3702 GN=CALS5 PE=1 SV=1[more]
Q9LTG51.1e-19562.39Callose synthase 4 OS=Arabidopsis thaliana OX=3702 GN=CALS4 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1JU281.2e-27986.031,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111488369 PE=3 SV=1[more]
A0A6J1GN911.2e-27986.031,3-beta-glucan synthase OS=Cucurbita moschata OX=3662 GN=LOC111455968 PE=3 SV=1[more]
A0A0A0K8F78.0e-27985.861,3-beta-glucan synthase OS=Cucumis sativus OX=3659 GN=Csa_7G429550 PE=3 SV=1[more]
A0A5A7UU132.3e-27884.741,3-beta-glucan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3CEN54.1e-27585.521,3-beta-glucan synthase OS=Cucumis melo OX=3656 GN=LOC103499883 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038898164.14.7e-28186.70callose synthase 1-like isoform X2 [Benincasa hispida][more]
XP_038898161.14.7e-28186.70callose synthase 1-like isoform X1 [Benincasa hispida] >XP_038898162.1 callose s... [more]
XP_022953403.12.6e-27986.03callose synthase 1 [Cucurbita moschata] >XP_022953404.1 callose synthase 1 [Cucu... [more]
KAG6575662.12.6e-27986.03Callose synthase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022991842.12.6e-27986.03callose synthase 1 [Cucurbita maxima] >XP_022991843.1 callose synthase 1 [Cucurb... [more]
Match NameE-valueIdentityDescription
AT5G13000.18.9e-23872.67glucan synthase-like 12 [more]
AT1G05570.11.0e-23371.31callose synthase 1 [more]
AT1G05570.21.0e-23371.31callose synthase 1 [more]
AT2G31960.11.8e-23070.97glucan synthase-like 3 [more]
AT2G31960.21.8e-23070.97glucan synthase-like 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003440Glycosyl transferase, family 48PFAMPF02364Glucan_synthasecoord: 1..345
e-value: 5.8E-112
score: 375.4
NoneNo IPR availablePANTHERPTHR12741LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1coord: 3..376
NoneNo IPR availablePANTHERPTHR12741LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1coord: 431..585
IPR026953Callose synthasePANTHERPTHR12741:SF70CALLOSE SYNTHASE 2coord: 3..376
IPR026953Callose synthasePANTHERPTHR12741:SF70CALLOSE SYNTHASE 2coord: 431..585

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G026140.1Lsi02G026140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006075 (1->3)-beta-D-glucan biosynthetic process
biological_process GO:0071555 cell wall organization
biological_process GO:0008360 regulation of cell shape
cellular_component GO:0000148 1,3-beta-D-glucan synthase complex
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0003843 1,3-beta-D-glucan synthase activity