Lsi02G025690 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G025690
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	carotenoid 9,10(9',10')-cleavage dioxygenase 1
Location	chr02: 32158962 .. 32159270 (+)
RNA-Seq Expression	Lsi02G025690
Synteny	Lsi02G025690

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGAGGAGAAGCAGAAGCTCAACGGCGGAGTCGCCGGACGATCCTTGGTGGAGGTGAATCCGAAGCCGAGTAAAGGGCTCACATCGAAGGCCACCGATTTGTTGGAGAAGCTGTTTGTCAAGCTTATGTATGATTCTTCAAGGCCTCAGCATTATCTTTCCGGTAATTTCGCTCCGGTTCACGATGAGACTCCTCCCATTACCGATCTTCCTGTTAAAGGTTATCTTCCGGTGAGTTGTAATCTGCTTTCTGATCTGGTGTTTGTTTATTCAGAACTGTTGCTTAATGAATTCAACTGGGTTTAG

mRNA sequence

ATGGCGGAGGAGAAGCAGAAGCTCAACGGCGGAGTCGCCGGACGATCCTTGGTGGAGGTGAATCCGAAGCCGAGTAAAGGGCTCACATCGAAGGCCACCGATTTGTTGGAGAAGCTGTTTGTCAAGCTTATGTATGATTCTTCAAGGCCTCAGCATTATCTTTCCGGTAATTTCGCTCCGGTTCACGATGAGACTCCTCCCATTACCGATCTTCCTGTTAAAGGTTATCTTCCGGTGAGTTGTAATCTGCTTTCTGATCTGGTGTTTGTTTATTCAGAACTGTTGCTTAATGAATTCAACTGGGTTTAG

Coding sequence (CDS)

ATGGCGGAGGAGAAGCAGAAGCTCAACGGCGGAGTCGCCGGACGATCCTTGGTGGAGGTGAATCCGAAGCCGAGTAAAGGGCTCACATCGAAGGCCACCGATTTGTTGGAGAAGCTGTTTGTCAAGCTTATGTATGATTCTTCAAGGCCTCAGCATTATCTTTCCGGTAATTTCGCTCCGGTTCACGATGAGACTCCTCCCATTACCGATCTTCCTGTTAAAGGTTATCTTCCGGTGAGTTGTAATCTGCTTTCTGATCTGGTGTTTGTTTATTCAGAACTGTTGCTTAATGAATTCAACTGGGTTTAG

Protein sequence

MAEEKQKLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAPVHDETPPITDLPVKGYLPVSCNLLSDLVFVYSELLLNEFNWV

Homology

[Close]

BLAST of Lsi02G025690 vs. ExPASy Swiss-Prot
Match: O65572 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=CCD1 PE=1 SV=2)

HSP 1 Score: 99.4 bits (246), Expect = 2.5e-20
Identity = 44/69 (63.77%), Postives = 55/69 (79.71%), Query Frame = 0


Query: 14 GRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAPVHDETPPITDLPV 73
          G S++ V+P+PSKG +SK  DLLE+L VKLM+D+S P HYLSGNFAP+ DETPP+ DLPV
Sbjct: 8  GSSIISVHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPV 67

Query: 74 KGYLPVSCN 83
           G+LP   N
Sbjct: 68 HGFLPECLN 76

[Close]

BLAST of Lsi02G025690 vs. ExPASy Swiss-Prot
Match: Q8LP17 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Pisum sativum OX=3888 GN=CCD1 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 7.4e-20
Identity = 45/66 (68.18%), Postives = 52/66 (78.79%), Query Frame = 0


Query: 17 LVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAPVHDETPPITDLPVKGY 76
          ++EV PKPS G TSKA DLLEK+ VKL YDSS P H+LSGNFAPV DETPP+ DL V+G+
Sbjct: 11 ILEVEPKPSNGFTSKAVDLLEKIIVKLFYDSSLPHHWLSGNFAPVKDETPPVKDLTVQGH 70

Query: 77 LPVSCN 83
          LP   N
Sbjct: 71 LPDCLN 76

[Close]

BLAST of Lsi02G025690 vs. ExPASy Swiss-Prot
Match: Q84KG5 (Carotenoid 9,10(9',10')-cleavage dioxygenase OS=Crocus sativus OX=82528 GN=CCD PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 2.8e-19
Identity = 45/68 (66.18%), Postives = 52/68 (76.47%), Query Frame = 0


Query: 15 RSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAPVHDETPPITDLPVK 74
          RS+V VNP+PSKGL S A DL+EK  V L +D S+P HYLSGNFAPV DETPP  DLPV+
Sbjct: 13 RSIVAVNPQPSKGLVSSAVDLIEKAVVYLFHDKSKPCHYLSGNFAPVVDETPPCPDLPVR 72

Query: 75 GYLPVSCN 83
          G+LP   N
Sbjct: 73 GHLPECLN 80

[Close]

BLAST of Lsi02G025690 vs. ExPASy Swiss-Prot
Match: Q94IR2 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Phaseolus vulgaris OX=3885 GN=CCD1 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 6.9e-18
Identity = 47/76 (61.84%), Postives = 54/76 (71.05%), Query Frame = 0


Query: 7  KLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAPVHDETP 66
          K NG   G  LV+V+PKP+ G +SK  DLLEKL VK +YDSS P HYL+GNF PV  ETP
Sbjct: 6  KKNGAEGG--LVKVDPKPTNGFSSKVIDLLEKLLVKFLYDSSLPHHYLTGNFGPV-TETP 65

Query: 67 PITDLPVKGYLPVSCN 83
          P  DLPVKG+LP   N
Sbjct: 66 PTKDLPVKGHLPDCLN 78

[Close]

BLAST of Lsi02G025690 vs. ExPASy Swiss-Prot
Match: C3VEQ4 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Oncidium hybrid cultivar OX=141207 GN=CCD1 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.0e-16
Identity = 42/79 (53.16%), Postives = 53/79 (67.09%), Query Frame = 0


Query: 4  EKQKLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAPVHD 63
          E++  +G  + RS+V V PKP KG+ S   DL+EK+ V   +DSS+P HYL+GNFAPV D
Sbjct: 19 EEEAADGKKSHRSVVAVQPKPRKGILSSTIDLIEKVIVYFAHDSSKPLHYLTGNFAPVRD 78

Query: 64 ETPPITDLPVKGYLPVSCN 83
          ETPP   L V G LPV  N
Sbjct: 79 ETPPFAALTVHGSLPVCLN 97

[Close]

BLAST of Lsi02G025690 vs. ExPASy TrEMBL
Match: A0A5A7UYJ0 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00940 PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 9.3e-34
Identity = 73/82 (89.02%), Postives = 77/82 (93.90%), Query Frame = 0


Query: 1  MAEEKQKLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAP 60
          MA+EKQKLNGG+ GRSLVEV+PKPSKGL SKATDLLEKLFVKLMYDSS+PQHYLSGNFAP
Sbjct: 1  MADEKQKLNGGLPGRSLVEVSPKPSKGLVSKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61 VHDETPPITDLPVKGYLPVSCN 83
          VHDETPPITDLPVKGYLP   N
Sbjct: 61 VHDETPPITDLPVKGYLPECLN 82

[Close]

BLAST of Lsi02G025690 vs. ExPASy TrEMBL
Match: A0A5D3CEY9 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00800 PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 9.3e-34
Identity = 73/82 (89.02%), Postives = 77/82 (93.90%), Query Frame = 0


Query: 1  MAEEKQKLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAP 60
          MA+EKQKLNGG+ GRSLVEV+PKPSKGL SKATDLLEKLFVKLMYDSS+PQHYLSGNFAP
Sbjct: 1  MADEKQKLNGGLPGRSLVEVSPKPSKGLVSKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61 VHDETPPITDLPVKGYLPVSCN 83
          VHDETPPITDLPVKGYLP   N
Sbjct: 61 VHDETPPITDLPVKGYLPECLN 82

[Close]

BLAST of Lsi02G025690 vs. ExPASy TrEMBL
Match: A0A1S3CFM3 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103499930 PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 9.3e-34
Identity = 73/82 (89.02%), Postives = 77/82 (93.90%), Query Frame = 0


Query: 1  MAEEKQKLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAP 60
          MA+EKQKLNGG+ GRSLVEV+PKPSKGL SKATDLLEKLFVKLMYDSS+PQHYLSGNFAP
Sbjct: 1  MADEKQKLNGGLPGRSLVEVSPKPSKGLVSKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61 VHDETPPITDLPVKGYLPVSCN 83
          VHDETPPITDLPVKGYLP   N
Sbjct: 61 VHDETPPITDLPVKGYLPECLN 82

[Close]

BLAST of Lsi02G025690 vs. ExPASy TrEMBL
Match: A0A6J1D5G1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Momordica charantia OX=3673 GN=LOC111017209 PE=3 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 7.9e-33
Identity = 73/82 (89.02%), Postives = 74/82 (90.24%), Query Frame = 0


Query: 1  MAEEKQKLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAP 60
          MAEEKQKLNGGV  RSLVEVNPKPSKGL SKATDLLEKLFVKLMYD+S PQHYLSGNFAP
Sbjct: 1  MAEEKQKLNGGVVDRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDASNPQHYLSGNFAP 60

Query: 61 VHDETPPITDLPVKGYLPVSCN 83
          V DETPPITDLPVKGYLP   N
Sbjct: 61 VRDETPPITDLPVKGYLPECLN 82

[Close]

BLAST of Lsi02G025690 vs. ExPASy TrEMBL
Match: K4K0F3 (Carotenoid cleavage dioxygenase 1 OS=Momordica charantia OX=3673 GN=CCD1 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 7.9e-33
Identity = 73/82 (89.02%), Postives = 74/82 (90.24%), Query Frame = 0


Query: 1  MAEEKQKLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAP 60
          MAEEKQKLNGGV  RSLVEVNPKPSKGL SKATDLLEKLFVKLMYD+S PQHYLSGNFAP
Sbjct: 1  MAEEKQKLNGGVVDRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDASNPQHYLSGNFAP 60

Query: 61 VHDETPPITDLPVKGYLPVSCN 83
          V DETPPITDLPVKGYLP   N
Sbjct: 61 VRDETPPITDLPVKGYLPECLN 82

[Close]

BLAST of Lsi02G025690 vs. NCBI nr
Match: XP_038899772.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Benincasa hispida])

HSP 1 Score: 152.9 bits (385), Expect = 1.5e-33
Identity = 74/82 (90.24%), Postives = 76/82 (92.68%), Query Frame = 0


Query: 1  MAEEKQKLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAP 60
          MAEEKQ L GGVAGRSLVEVNPKPSKGL SKATDLLE+LFVKLMYDSS+PQHYLSGNFAP
Sbjct: 1  MAEEKQNLKGGVAGRSLVEVNPKPSKGLASKATDLLEQLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61 VHDETPPITDLPVKGYLPVSCN 83
          VHDETPPITDLPVKGYLP   N
Sbjct: 61 VHDETPPITDLPVKGYLPECLN 82

[Close]

BLAST of Lsi02G025690 vs. NCBI nr
Match: KAA0058625.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 152.5 bits (384), Expect = 1.9e-33
Identity = 73/82 (89.02%), Postives = 77/82 (93.90%), Query Frame = 0


Query: 1  MAEEKQKLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAP 60
          MA+EKQKLNGG+ GRSLVEV+PKPSKGL SKATDLLEKLFVKLMYDSS+PQHYLSGNFAP
Sbjct: 1  MADEKQKLNGGLPGRSLVEVSPKPSKGLVSKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61 VHDETPPITDLPVKGYLPVSCN 83
          VHDETPPITDLPVKGYLP   N
Sbjct: 61 VHDETPPITDLPVKGYLPECLN 82

[Close]

BLAST of Lsi02G025690 vs. NCBI nr
Match: XP_008461300.1 (PREDICTED: carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Cucumis melo])

HSP 1 Score: 152.5 bits (384), Expect = 1.9e-33
Identity = 73/82 (89.02%), Postives = 77/82 (93.90%), Query Frame = 0


Query: 1  MAEEKQKLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAP 60
          MA+EKQKLNGG+ GRSLVEV+PKPSKGL SKATDLLEKLFVKLMYDSS+PQHYLSGNFAP
Sbjct: 1  MADEKQKLNGGLPGRSLVEVSPKPSKGLVSKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61 VHDETPPITDLPVKGYLPVSCN 83
          VHDETPPITDLPVKGYLP   N
Sbjct: 61 VHDETPPITDLPVKGYLPECLN 82

[Close]

BLAST of Lsi02G025690 vs. NCBI nr
Match: TYK10433.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 152.5 bits (384), Expect = 1.9e-33
Identity = 73/82 (89.02%), Postives = 77/82 (93.90%), Query Frame = 0


Query: 1  MAEEKQKLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAP 60
          MA+EKQKLNGG+ GRSLVEV+PKPSKGL SKATDLLEKLFVKLMYDSS+PQHYLSGNFAP
Sbjct: 1  MADEKQKLNGGLPGRSLVEVSPKPSKGLVSKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61 VHDETPPITDLPVKGYLPVSCN 83
          VHDETPPITDLPVKGYLP   N
Sbjct: 61 VHDETPPITDLPVKGYLPECLN 82

[Close]

BLAST of Lsi02G025690 vs. NCBI nr
Match: XP_022148582.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Momordica charantia])

HSP 1 Score: 149.4 bits (376), Expect = 1.6e-32
Identity = 73/82 (89.02%), Postives = 74/82 (90.24%), Query Frame = 0


Query: 1  MAEEKQKLNGGVAGRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAP 60
          MAEEKQKLNGGV  RSLVEVNPKPSKGL SKATDLLEKLFVKLMYD+S PQHYLSGNFAP
Sbjct: 1  MAEEKQKLNGGVVDRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDASNPQHYLSGNFAP 60

Query: 61 VHDETPPITDLPVKGYLPVSCN 83
          V DETPPITDLPVKGYLP   N
Sbjct: 61 VRDETPPITDLPVKGYLPECLN 82

[Close]

BLAST of Lsi02G025690 vs. TAIR 10
Match: AT3G63520.1 (carotenoid cleavage dioxygenase 1 )

HSP 1 Score: 99.4 bits (246), Expect = 1.8e-21
Identity = 44/69 (63.77%), Postives = 55/69 (79.71%), Query Frame = 0


Query: 14 GRSLVEVNPKPSKGLTSKATDLLEKLFVKLMYDSSRPQHYLSGNFAPVHDETPPITDLPV 73
          G S++ V+P+PSKG +SK  DLLE+L VKLM+D+S P HYLSGNFAP+ DETPP+ DLPV
Sbjct: 8  GSSIISVHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPV 67

Query: 74 KGYLPVSCN 83
           G+LP   N
Sbjct: 68 HGFLPECLN 76

The following BLAST results are available for this feature:

BLAST of Lsi02G025690 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65572	2.5e-20	63.77	Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q8LP17	7.4e-20	68.18	Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Pisum sativum OX=3888 GN=CCD1 ...	[more]
Q84KG5	2.8e-19	66.18	Carotenoid 9,10(9',10')-cleavage dioxygenase OS=Crocus sativus OX=82528 GN=CCD P...	[more]
Q94IR2	6.9e-18	61.84	Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Phaseolus vulgaris OX=3885 GN=...	[more]
C3VEQ4	1.0e-16	53.16	Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Oncidium hybrid cultivar OX=14...	[more]

BLAST of Lsi02G025690 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UYJ0	9.3e-34	89.02	Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A5D3CEY9	9.3e-34	89.02	Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3CFM3	9.3e-34	89.02	carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6J1D5G1	7.9e-33	89.02	carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Momordica charantia OX=3673 GN...	[more]
K4K0F3	7.9e-33	89.02	Carotenoid cleavage dioxygenase 1 OS=Momordica charantia OX=3673 GN=CCD1 PE=2 SV...	[more]

BLAST of Lsi02G025690 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899772.1	1.5e-33	90.24	carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Benincasa hispida]	[more]
KAA0058625.1	1.9e-33	89.02	carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Cucumis melo var. makuwa]	[more]
XP_008461300.1	1.9e-33	89.02	PREDICTED: carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Cucumis melo]	[more]
TYK10433.1	1.9e-33	89.02	carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Cucumis melo var. makuwa]	[more]
XP_022148582.1	1.6e-32	89.02	carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Momordica charantia]	[more]

BLAST of Lsi02G025690 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63520.1	1.8e-21	63.77	carotenoid cleavage dioxygenase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004294	Carotenoid oxygenase	PANTHER	PTHR10543	BETA-CAROTENE DIOXYGENASE	coord: 14..82
None	No IPR available	PANTHER	PTHR10543:SF124	CAROTENOID CLEAVAGE DIOXYGENASE 1-3	coord: 14..82

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G025690.1	Lsi02G025690.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016121	carotene catabolic process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0010436	carotenoid dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen