Lsi02G025620 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G025620
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDNA-directed RNA polymerases II, IV and V subunit 11-like
Locationchr02: 32104045 .. 32106864 (-)
RNA-Seq ExpressionLsi02G025620
SyntenyLsi02G025620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAGCCAAACCACGTCGCCTATCTTCATATCGCTATTTCAAGAGAGAGTTTCAGTTGCAGAGAGAAAAAGGAGGAGAAGAAGAAAGACGATGAATGTACCCGATCGCTACGAGCGCTTCGTCGTCCCCGAGGGCGCCAAGAAGTTAATTCTTGAAACCCTAACTTTTTCATCATCTCTTTCATTTCACGACCGATTTTGCTTTCTTTCTATATCCACCACAACCATCTCTGTTCTGATCTTCTTTTCTGGACGGACGTCGATTTGTTGAACATTGTAGGGTTTCATACGAGAGAGACACGAAGATCATGAATGCGGCGACCTTTATATTAGAGAGAGAGGATCATACTATTGGAAATATCCTCCGCATGTAAGGGATTTCTGTTAATCGGAGATAGTTTCTGGCGATTTTAAATAATTTATTCGTTCTTGTTTATTAACATGTATTATGGCCTGCATAATCAGGCAACTTCACAGAGATGATAATGTGTTGTTTGCTGCCTACCAGCTTCCTCATCCTCTGAAGTACAGAATACTCGTTCGGGTTAGTACTTTGATTATTTTTCCACGGTATAGTTTTTGCTCTATTGCATGATTGGTTAAATGAATTGTATTGTGAGCATCTTTGCAAAGCAATGTTGCTGTTTGGTAATGGGGGATTGTAATCTGGAGGAGAATAATTGATCAAATTTTATTACGTAATTAGCGTTGATTTGTTCTTATAGTTTTCGGTTATTTGAGATTGACCGTATCGAATGAGAATTTCCTTGACTGGCAGGCAGAGCAGAAATAACTTTTCACGGGAAATTTCAAATTATATCTTCTAAGTACTTTTTATGATGAAATATTTTAAATCTCAACTTTTGTTTGTATAATTTTCTCCGTCTTAGATGAATTAGGTAATTGTCTCTCCTCTATTCTTCATTCGGCTCTTCGACTGGAGTTCAAATGACCAATTTGCCTCGGTGTAAGTGAAACTTTAGTTGAATGCTTAAGCTCCTTATCCAATTTTAGTAACATTTTCGTTTTTCCCCTAGAAAGATCATGAACTCCTGGGGACAAAACCCCGATTTGAAATTCCTTGAGGATAGCAAAGATTTTGCAGATACTGATTGGTTTTAAATACTGCGATTCTATAGAGCTTTATTGTGGTCTGTTATTGATTAAATTACAAAACCCCGATTTGAAATTCCTTGACGATATTATTTTATCATAATTTTTTTTTAAAAAAATTTCGATGTAATTTTTCTGCAGATACAAACTGCTAGCCAGTCTTCACCGATGCAGGCATATAATCAGGCTGTCAATGATTTGGACAAGGAGCTTGATGTTTTGAAATATGAGCTTGAGGTAATGCTTTCCCTCCCCCTTGACTATTTATTTCTGCAATTTAATTGGTGCGGGTATGATTTGCTCTTATTCATTATGAGGGAATCCAGTCCTCTGATTATTTTGCTCTTAAGGTTTACACCTATTTTCTTTTGTTTTTGTTCTTATCTTTTTAAATGATTGATTTGTTTTAGTACTAATATGGAAATTTGAATATCCTACTTTAAGGAAAGGAATACACATGACCACGCTCGCTTTGCTCTAAAGGATTAATTTTGATACTCGATCTTTGAGTAAAAATTTGTGTGGTCTTGTTAAATGTTCATATTACATTTTAAACTCCAACATGGATGGGATACATGTAGTTTATAATACGAGTTCATTTCAGATTATTTTAAGCTTTTGTTAAATGCAGCATAAGAATTTAATAGTCAAAGAGAAAAAGAACTTTTCTCCAAAGTTTAAGGGATAAATATTTACAAGTCTAAATATCTAAAAGGAAAAGAAGAAGAAGAAGAAGAAGAAGAATAAGATAATGTCATTATTATTATTATTATTTACATTTGTGAATTGCTTGACGCAGGGTGAGATTTCAAGGTTCTCGAGGACCTATTAGAGTTTGCCTTTCAAGATAACGAAGGGAAATTTCGAACTCAACAAAGTCGAGTTGGAAATGGAATGTCAAGAATAAAATTATTGGCCACTTTTCAGTTCGGCTCCTTTTGGTGGGAGTCGATTGTATTCTTTCTTTCTTTTAAATGAAAGTTCAAAAATTTATCCCAAAAAAAGCCATTTTAATTTATTATTGATTTGAAATAAAATTTAGGCCAAATTATAGAAAATATTTTGAACCTCATTTATAATAACTCACTCTTAAGTTTCATTGAAATTATTGTCCTTTAAAAAGAAATTTGAAAAATACCCTTTATTGTGAGTAGTGGGACGATAAATGTTCATCTTTTCTTCCCATCTCTCCAAATTCTTCCTACAGGAAAATTAAATTAGAGCTTAATGGCAATCTTCACCACCAGGAGACAATACCAAATGGAAGAGAAACGAAGCCCAAAAAGCGCTGCAAATTGGTGATATATAAGCTATATTCACCACCATGATTACACCAAATGGAAGAGATAGAGCTCAAAACAAGGGCTTTCATCCAACGCCATGAAAATAGCTCAAAGATATTAACAGCAGGGATAGATTTAGTATTTGCTCGGGGACTGAGTCTTCTTCAACTCTATGCTCTTTATATATATATATATATATATAATTGACATAGTATCAATACCATTAGCTACGTTAGTGATAAATCTGTCTTCTGGCCTCAATTAGACTCAAGTTCAAGCTCTGCTCTTTTGCATTTTAGAAATTTTTTTAGTACCACGAGAGTATCATATTTTCTTTTAAATTTATCTTAATAAATATTAGTAGACTCTGTATGAATACAAGCGGATCTATCTCATTTTCAAAGAAAGTTTTCCCATTACCCAG

mRNA sequence

AAAGAGCCAAACCACGTCGCCTATCTTCATATCGCTATTTCAAGAGAGAGTTTCAGTTGCAGAGAGAAAAAGGAGGAGAAGAAGAAAGACGATGAATGTACCCGATCGCTACGAGCGCTTCGTCGTCCCCGAGGGCGCCAAGAAGGTTTCATACGAGAGAGACACGAAGATCATGAATGCGGCGACCTTTATATTAGAGAGAGAGGATCATACTATTGGAAATATCCTCCGCATGCAACTTCACAGAGATGATAATGTGTTGTTTGCTGCCTACCAGCTTCCTCATCCTCTGAAGTACAGAATACTCGTTCGGATACAAACTGCTAGCCAGTCTTCACCGATGCAGGCATATAATCAGGCTGTCAATGATTTGGACAAGGAGCTTGATGTTTTGAAATATGAGCTTGAGGTAATGCTTTCCCTCCCCCTTGACTATTTATTTCTGCAATTTAATTGGTGCGGGGTGAGATTTCAAGGTTCTCGAGGACCTATTAGAGTTTGCCTTTCAAGATAACGAAGGGAAATTTCGAACTCAACAAAGTCGAGTTGGAAATGGAATGTCAAGAATAAAATTATTGGCCACTTTTCAGTTCGGCTCCTTTTGGTGGGAGTCGATTGTATTCTTTCTTTCTTTTAAATGAAAGTTCAAAAATTTATCCCAAAAAAAGCCATTTTAATTTATTATTGATTTGAAATAAAATTTAGGCCAAATTATAGAAAATATTTTGAACCTCATTTATAATAACTCACTCTTAAGTTTCATTGAAATTATTGTCCTTTAAAAAGAAATTTGAAAAATACCCTTTATTGTGAGTAGTGGGACGATAAATGTTCATCTTTTCTTCCCATCTCTCCAAATTCTTCCTACAGGAAAATTAAATTAGAGCTTAATGGCAATCTTCACCACCAGGAGACAATACCAAATGGAAGAGAAACGAAGCCCAAAAAGCGCTGCAAATTGGTGATATATAAGCTATATTCACCACCATGATTACACCAAATGGAAGAGATAGAGCTCAAAACAAGGGCTTTCATCCAACGCCATGAAAATAGCTCAAAGATATTAACAGCAGGGATAGATTTAGTATTTGCTCGGGGACTGAGTCTTCTTCAACTCTATGCTCTTTATATATATATATATATATATAATTGACATAGTATCAATACCATTAGCTACGTTAGTGATAAATCTGTCTTCTGGCCTCAATTAGACTCAAGTTCAAGCTCTGCTCTTTTGCATTTTAGAAATTTTTTTAGTACCACGAGAGTATCATATTTTCTTTTAAATTTATCTTAATAAATATTAGTAGACTCTGTATGAATACAAGCGGATCTATCTCATTTTCAAAGAAAGTTTTCCCATTACCCAG

Coding sequence (CDS)

ATGAATGTACCCGATCGCTACGAGCGCTTCGTCGTCCCCGAGGGCGCCAAGAAGGTTTCATACGAGAGAGACACGAAGATCATGAATGCGGCGACCTTTATATTAGAGAGAGAGGATCATACTATTGGAAATATCCTCCGCATGCAACTTCACAGAGATGATAATGTGTTGTTTGCTGCCTACCAGCTTCCTCATCCTCTGAAGTACAGAATACTCGTTCGGATACAAACTGCTAGCCAGTCTTCACCGATGCAGGCATATAATCAGGCTGTCAATGATTTGGACAAGGAGCTTGATGTTTTGAAATATGAGCTTGAGGTAATGCTTTCCCTCCCCCTTGACTATTTATTTCTGCAATTTAATTGGTGCGGGGTGAGATTTCAAGGTTCTCGAGGACCTATTAGAGTTTGCCTTTCAAGATAA

Protein sequence

MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAAYQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELEVMLSLPLDYLFLQFNWCGVRFQGSRGPIRVCLSR
Homology
BLAST of Lsi02G025620 vs. ExPASy Swiss-Prot
Match: Q38859 (DNA-directed RNA polymerases II, IV and V subunit 11 OS=Arabidopsis thaliana OX=3702 GN=NRPB11 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.4e-45
Identity = 86/106 (81.13%), Postives = 97/106 (91.51%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MN P+RYERFVVPEG KKVSY+RDTKI+NAA+F +EREDHTIGNI+RMQLHRD+NVLFA 
Sbjct: 1   MNAPERYERFVVPEGTKKVSYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELE 107
           YQLPHPLKY+I+VRI T SQSSPMQAYNQA+NDLDKELD LK + E
Sbjct: 61  YQLPHPLKYKIIVRIHTTSQSSPMQAYNQAINDLDKELDYLKNQFE 106

BLAST of Lsi02G025620 vs. ExPASy Swiss-Prot
Match: Q86JJ5 (DNA-directed RNA polymerase II subunit rpb11 OS=Dictyostelium discoideum OX=44689 GN=polr2j PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.3e-24
Identity = 49/102 (48.04%), Postives = 77/102 (75.49%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MN PDR+E FV+P+GAKKV+  RDTKI NA+ F + +EDHTIGN++RMQL  D +++FA 
Sbjct: 1   MNAPDRFELFVLPDGAKKVTMVRDTKIPNASMFTILKEDHTIGNLIRMQLIADQDIIFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLK 103
           Y++PHPL++ + +RIQT + ++P++   +++  L +E   L+
Sbjct: 61  YRMPHPLEHNVNIRIQTNNNTNPLECVQKSMECLSREFTSLE 102

BLAST of Lsi02G025620 vs. ExPASy Swiss-Prot
Match: Q32P79 (DNA-directed RNA polymerase II subunit RPB11 OS=Bos taurus OX=9913 GN=POLR2J PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 5.2e-24
Identity = 48/109 (44.04%), Postives = 75/109 (68.81%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MN P  +E F++ EG KK++  +DTK+ NA  F + +EDHT+GNI++ QL +D  VLFA 
Sbjct: 1   MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELEVML 110
           Y++PHPL+++I++R+QT    SP +A+  A+ DL  EL +L+    V +
Sbjct: 61  YKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAI 109

BLAST of Lsi02G025620 vs. ExPASy Swiss-Prot
Match: P52435 (DNA-directed RNA polymerase II subunit RPB11-a OS=Homo sapiens OX=9606 GN=POLR2J PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 5.2e-24
Identity = 48/109 (44.04%), Postives = 75/109 (68.81%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MN P  +E F++ EG KK++  +DTK+ NA  F + +EDHT+GNI++ QL +D  VLFA 
Sbjct: 1   MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELEVML 110
           Y++PHPL+++I++R+QT    SP +A+  A+ DL  EL +L+    V +
Sbjct: 61  YKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAI 109

BLAST of Lsi02G025620 vs. ExPASy Swiss-Prot
Match: O08740 (DNA-directed RNA polymerase II subunit RPB11 OS=Mus musculus OX=10090 GN=Polr2j PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.2e-23
Identity = 47/102 (46.08%), Postives = 73/102 (71.57%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MN P  +E F++ EG KK++  +DTK+ NA  F + +EDHT+GNI++ QL +D  VLFA 
Sbjct: 1   MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLK 103
           Y++PHPL+++I++R+QT    SP +A+  A+ DL  EL +L+
Sbjct: 61  YKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLE 102

BLAST of Lsi02G025620 vs. ExPASy TrEMBL
Match: A0A5D3CJ64 (DNA-directed RNA polymerases II, IV and V subunit 11-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00720 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.2e-61
Identity = 124/135 (91.85%), Postives = 126/135 (93.33%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA 
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELEVMLSLPLDYLF-LQ 120
           YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDVLKYELEVMLSLPLD+ F L 
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELEVMLSLPLDFFFSLS 120

Query: 121 FNWCGVRFQGSRGPI 135
                VRFQGSRGPI
Sbjct: 121 AIGVRVRFQGSRGPI 135

BLAST of Lsi02G025620 vs. ExPASy TrEMBL
Match: A0A5A7UV57 (DNA-directed RNA polymerases II, IV and V subunit 11-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00860 PE=3 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 2.6e-58
Identity = 116/125 (92.80%), Postives = 118/125 (94.40%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA 
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELEVMLSLPLDYLFLQF 120
           YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDVLKYELEVMLSLPLD+ F   
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELEVMLSLPLDFFFSLC 120

Query: 121 NWCGV 126
           NW  V
Sbjct: 121 NWSEV 125

BLAST of Lsi02G025620 vs. ExPASy TrEMBL
Match: A0A1S3CFN3 (DNA-directed RNA polymerases II, IV and V subunit 11-like OS=Cucumis melo OX=3656 GN=LOC103499938 PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.0e-51
Identity = 105/110 (95.45%), Postives = 107/110 (97.27%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA 
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELEVMLS 111
           YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDVLKYELE  +S
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELESEIS 110

BLAST of Lsi02G025620 vs. ExPASy TrEMBL
Match: A0A6J1KX24 (DNA-directed RNA polymerases II, IV and V subunit 11-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497917 PE=3 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.3e-51
Identity = 103/106 (97.17%), Postives = 106/106 (100.00%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFI+EREDHTIGNI+RMQLHRDDNVLFAA
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFIVEREDHTIGNIIRMQLHRDDNVLFAA 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELE 107
           YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDVLKYELE
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELE 106

BLAST of Lsi02G025620 vs. ExPASy TrEMBL
Match: A0A6J1H8G6 (DNA-directed RNA polymerases II, IV and V subunit 11-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461018 PE=3 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.3e-51
Identity = 103/106 (97.17%), Postives = 106/106 (100.00%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFI+EREDHTIGNI+RMQLHRDDNVLFAA
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFIVEREDHTIGNIIRMQLHRDDNVLFAA 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELE 107
           YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDVLKYELE
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELE 106

BLAST of Lsi02G025620 vs. NCBI nr
Match: TYK10426.1 (DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis melo var. makuwa])

HSP 1 Score: 244.2 bits (622), Expect = 6.7e-61
Identity = 124/135 (91.85%), Postives = 126/135 (93.33%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA 
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELEVMLSLPLDYLF-LQ 120
           YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDVLKYELEVMLSLPLD+ F L 
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELEVMLSLPLDFFFSLS 120

Query: 121 FNWCGVRFQGSRGPI 135
                VRFQGSRGPI
Sbjct: 121 AIGVRVRFQGSRGPI 135

BLAST of Lsi02G025620 vs. NCBI nr
Match: KAA0058617.1 (DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis melo var. makuwa])

HSP 1 Score: 234.6 bits (597), Expect = 5.3e-58
Identity = 116/125 (92.80%), Postives = 118/125 (94.40%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA 
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELEVMLSLPLDYLFLQF 120
           YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDVLKYELEVMLSLPLD+ F   
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELEVMLSLPLDFFFSLC 120

Query: 121 NWCGV 126
           NW  V
Sbjct: 121 NWSEV 125

BLAST of Lsi02G025620 vs. NCBI nr
Match: XP_008461312.1 (PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis melo])

HSP 1 Score: 212.6 bits (540), Expect = 2.2e-51
Identity = 105/110 (95.45%), Postives = 107/110 (97.27%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA 
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELEVMLS 111
           YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDVLKYELE  +S
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELESEIS 110

BLAST of Lsi02G025620 vs. NCBI nr
Match: XP_004135939.1 (DNA-directed RNA polymerases II, IV and V subunit 11 isoform X3 [Cucumis sativus] >KAE8646398.1 hypothetical protein Csa_016830 [Cucumis sativus])

HSP 1 Score: 212.2 bits (539), Expect = 2.8e-51
Identity = 105/110 (95.45%), Postives = 107/110 (97.27%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFA 
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELEVMLS 111
           YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDVLKYELE  +S
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELEGEIS 110

BLAST of Lsi02G025620 vs. NCBI nr
Match: XP_023004699.1 (DNA-directed RNA polymerases II, IV and V subunit 11-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 211.5 bits (537), Expect = 4.8e-51
Identity = 103/106 (97.17%), Postives = 106/106 (100.00%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFI+EREDHTIGNI+RMQLHRDDNVLFAA
Sbjct: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFIVEREDHTIGNIIRMQLHRDDNVLFAA 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELE 107
           YQLPHPLKYRILVRIQTASQSSPMQAYNQA+NDLDKELDVLKYELE
Sbjct: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAINDLDKELDVLKYELE 106

BLAST of Lsi02G025620 vs. TAIR 10
Match: AT3G52090.1 (DNA-directed RNA polymerase, RBP11-like )

HSP 1 Score: 183.7 bits (465), Expect = 1.0e-46
Identity = 86/106 (81.13%), Postives = 97/106 (91.51%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MN P+RYERFVVPEG KKVSY+RDTKI+NAA+F +EREDHTIGNI+RMQLHRD+NVLFA 
Sbjct: 1   MNAPERYERFVVPEGTKKVSYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAG 60

Query: 61  YQLPHPLKYRILVRIQTASQSSPMQAYNQAVNDLDKELDVLKYELE 107
           YQLPHPLKY+I+VRI T SQSSPMQAYNQA+NDLDKELD LK + E
Sbjct: 61  YQLPHPLKYKIIVRIHTTSQSSPMQAYNQAINDLDKELDYLKNQFE 106

BLAST of Lsi02G025620 vs. TAIR 10
Match: AT3G52090.2 (DNA-directed RNA polymerase, RBP11-like )

HSP 1 Score: 167.9 bits (424), Expect = 5.7e-42
Identity = 86/136 (63.24%), Postives = 97/136 (71.32%), Query Frame = 0

Query: 1   MNVPDRYERFVVPEGAKKVSYERDTKIMNAATFILEREDHTIGNILRMQLHRDDNVLFAA 60
           MN P+RYERFVVPEG KKVSY+RDTKI+NAA+F +EREDHTIGNI+RMQLHRD+NVLFA 
Sbjct: 1   MNAPERYERFVVPEGTKKVSYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAG 60

Query: 61  YQLPHPLKYRILVR------------------------------IQTASQSSPMQAYNQA 107
           YQLPHPLKY+I+VR                              I T SQSSPMQAYNQA
Sbjct: 61  YQLPHPLKYKIIVRDPKDWFRNLKVVYGCLETVNISWFKLLVLTIHTTSQSSPMQAYNQA 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q388591.4e-4581.13DNA-directed RNA polymerases II, IV and V subunit 11 OS=Arabidopsis thaliana OX=... [more]
Q86JJ52.3e-2448.04DNA-directed RNA polymerase II subunit rpb11 OS=Dictyostelium discoideum OX=4468... [more]
Q32P795.2e-2444.04DNA-directed RNA polymerase II subunit RPB11 OS=Bos taurus OX=9913 GN=POLR2J PE=... [more]
P524355.2e-2444.04DNA-directed RNA polymerase II subunit RPB11-a OS=Homo sapiens OX=9606 GN=POLR2J... [more]
O087401.2e-2346.08DNA-directed RNA polymerase II subunit RPB11 OS=Mus musculus OX=10090 GN=Polr2j ... [more]
Match NameE-valueIdentityDescription
A0A5D3CJ643.2e-6191.85DNA-directed RNA polymerases II, IV and V subunit 11-like OS=Cucumis melo var. m... [more]
A0A5A7UV572.6e-5892.80DNA-directed RNA polymerases II, IV and V subunit 11-like OS=Cucumis melo var. m... [more]
A0A1S3CFN31.0e-5195.45DNA-directed RNA polymerases II, IV and V subunit 11-like OS=Cucumis melo OX=365... [more]
A0A6J1KX242.3e-5197.17DNA-directed RNA polymerases II, IV and V subunit 11-like isoform X1 OS=Cucurbit... [more]
A0A6J1H8G62.3e-5197.17DNA-directed RNA polymerases II, IV and V subunit 11-like isoform X1 OS=Cucurbit... [more]
Match NameE-valueIdentityDescription
TYK10426.16.7e-6191.85DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis melo var. mak... [more]
KAA0058617.15.3e-5892.80DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis melo var. mak... [more]
XP_008461312.12.2e-5195.45PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 11-like [Cucumis me... [more]
XP_004135939.12.8e-5195.45DNA-directed RNA polymerases II, IV and V subunit 11 isoform X3 [Cucumis sativus... [more]
XP_023004699.14.8e-5197.17DNA-directed RNA polymerases II, IV and V subunit 11-like isoform X1 [Cucurbita ... [more]
Match NameE-valueIdentityDescription
AT3G52090.11.0e-4681.13DNA-directed RNA polymerase, RBP11-like [more]
AT3G52090.25.7e-4263.24DNA-directed RNA polymerase, RBP11-like [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 84..104
NoneNo IPR availablePANTHERPTHR13946:SF16DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11coord: 1..107
NoneNo IPR availablePANTHERPTHR13946DNA-DIRECTED RNA POLYMERASE I,II,IIIcoord: 1..107
IPR009025DNA-directed RNA polymerase, RBP11-like dimerisation domainPFAMPF13656RNA_pol_L_2coord: 31..102
e-value: 1.0E-23
score: 82.9
IPR036603RNA polymerase, RBP11-like subunitGENE3D3.30.1360.10coord: 1..111
e-value: 5.5E-47
score: 160.3
IPR036603RNA polymerase, RBP11-like subunitSUPERFAMILY55257RBP11-like subunits of RNA polymerasecoord: 1..106
IPR008193DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved sitePROSITEPS01154RNA_POL_L_13KDcoord: 35..66
IPR037685RNA polymerase RBP11CDDcd06926RNAP_II_RPB11coord: 13..102
e-value: 9.01324E-48
score: 147.354

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G025620.1Lsi02G025620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006366 transcription by RNA polymerase II
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005665 RNA polymerase II, core complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0001055 RNA polymerase II activity
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity