Lsi02G025580 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G025580
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptiondisease resistance protein RGA2
Locationchr02: 32060051 .. 32063152 (+)
RNA-Seq ExpressionLsi02G025580
SyntenyLsi02G025580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGAAGCAATTCTCTTCGATGTTGCTGGGGCGATCTTGATGAAGCTAAGCTCTCAAGCTTTCCAGCGGCTTGGGATGCTATTTGGGCTGAAAGATGATCTTAACAAACTCACAGGCACAGTTTCAACCATTAAGGATGTGCTTCTTGATGCGGAGGGGCGTCAAACTAAAAGTCACTTGCTAGAGAATTGGCTCCAGAAATTGGAAGAAGCTCTTCATGATGCGGAGGATGTGCTTGATGAACTCTCTACGGAGGCTCTTCATCGAGAAGTGATGACTAGAGATAAAAATGCAAAACAAGTAAGGATCTTCTTCTCCAAATCTAATCAACTAGCACTTAATTATAGGATGGCTCGTCAAATAAAGAAAATTTGGGAAAGGCTAGATGCAATTGATGCTGAAAAAAAACAATTTCATTTGCATGAAAACTGTGAATCAAAGACTCGATACGGTTCATTTGATCAAATAATGACTGGAAGGGAATCTTGGTCTTTTTCAAATGAAGAGGAAGTGATTGGGAGGGATGATGATAAGAAAAAGGTAAAGGACCTTCTATTGGATGTAAATATGAATGTCACACACAATGTTTCATTCGTTGCCATAGTTGGGATGGGTGGAATAGGCAAGACAACCTTGGCTAAATCTCTCTACAATGATGAAAAATTATCAAAATATTTTGAGTTAAAAATATGGGTTTGGGTTTCTGATCAATTTGAGATAAAAGCGGTAGTGGAGAAAATAATAGAATCTGCAACCAAAAGCAATCCTCATGTAAAAGGAATGGAAGCTTTACAAACAGAGCTTCAAGAAGTGATTGGGGGAAAGAAGTATTTGCTAGTTATGGACGATGTATGGAATGAAAGTGAAGAGAAATGGCATGGGTTGAAACCTTTGTTAATGAGTGGTGCGAAAGGGAGTAAGATTTTGATCACAAAGCGTGATAGCAAAGTAGCTGCAGAAATTGAAAGCATGACTTCTTTATTCACTTTAGAAGGCTTACCGGATGGTAAATCTTGGTCATTATTTAGTAAAGTGGCATTTAAAGAAGGCAAAGAGGTTGAAAATCCAAACTTGATACATTTAGGAAAAGAAATTTTAGTGAAATCTGGGGGTGTTCCTCTTGTAATAAGACATATTGGCCGCTTGCTATACTCAAAAACTTCAGAAGAAGAATGGATGTCTTTCAAGAATAATGAACTTTTAGAGATCACTCAGCAAGACAATGATATGACATCGGTATTAAAATTGAGCTATAACCATCTCCCACCAAATCTGAAGCAGTGTTTTGCCTATTTATCCTTGTTTCCCAAAGGGAAGAGCTTAAAAATAAATGATTTGATTAGACAATGGATGGCTCTAGGTTTTATAGAATCATCAAATGGAACTAAATGCATGGAAGATACGGGAAATGACTATTTCAAGGAATTGTGTTGGAGGTTTTTTTTGGAAAATTCCATCAATGAGTGTAACTTCGACAATGATGTCCACGTGCACGATGTGATGCGTGATCTTGCAACAAATGTAGCAGGAAAGAAATATGTATGTGAAAATCTCAATCGTGATTATGCTTTCAATGAAAAAACTCGCCATGTTTCATTTGATTGTAAAATAAAATCATGGTCAGATGTTCTATCCAAATTACGCACGGCAAAAGGATTAAGAACGTTCCAACTATTCTCATCCAATTTTGAATATGAAAAGAAGAATGAGATCAATGAAGCTATTTTGGATGAAGTATTTTCCAGTTTTCCACGTTTGCGAGTATTGGGTCTTAATAACTCAAATAGTCGCACAGTGCCAAATTCTATAAGAAGGCTTAGACATCTTCGATATCTAGACCTCTCTGAAAATAATATGGAATCACTTCCAAATTCTATCACTGAATTGCAAAACTTGCAAACTCTAAATCTAATGTACTGTAGAGAACTAAAGGAATTGCCCAGGGACACCAGAAATCTTGTTAATCTTAGGCATCTTGATTTCAATACTTCTACCTTAACCCATATGCCAGAAGGGATAGGGAGGTTGAGTTGTCTACAGACACTAAGTTATTTTGTGTTGGACTGCAAAAGGTCTAGTCAGCTGAGTGAATTGAATGTGCTGATCCATTTAAATGGAGATCTAAGAATTATTGGTTTAGAGTATTTGAGGCATAATCCATCTGAAGTCAGCTTAGTAAACCTGAAAGACATAAAAGGCTTGAAGAATCTGCAACTGGAATGGAAACTGAGCCGAGATGATCAAGAATATGAAGGTGAGGATGATGAAACTGCAATAATGGAAGGCCTAGAACCACATCCAAATGTTGAATCCTTGCGCATTGACGGGTACAGTGGAGTAGGATTAACCAATTGGGTGTCCAGCTCCCTCTCAAAGTTAACTAAAATTATAATTTATAAATGTCATAGATTGCAACATCTAACTCAAATGGGCCATCTTCAGGCTCTCACATTTCTATATTTGGATGACATGAGATCTCTCAAGTTCATAGACAAGAATGAACCATCCTCCTCATCCTCTTTCCCATCTCTCGAGCTTCTAATTATCGCAAACATGCCTAATTTGGAAGGATGGTGGGAATTAGGGGAGACTCAAAAAAATTGGTTGCCTCCAACTTTTCCTAAGCTTATTTCCCTGCATATCTCTGGGTGTCCTAAGTTAAGTTTGATGCCCAAGCCAGCTTCCACTGGAGCAGTTGTGTTTTTATGTGATGTGGGTGTTCAATTGGTGAGTGCATTAGGTCCATTGTGGGGTTTGAAGAGTCTAACACTGGAGGAAATCGAGGATCTCAAATATCTAGAAATCATGCAAAACCTCGGTTCTTTGCCTATACAACTTCGACATTTGAAAATAAGGAAATGCTCAAATTTGATGAGTTTACCTGAATGGATTGGCATCCTCACTTCGCTTGAGGACCTGGAGATTATGGAATGTCCGAAATTAAAATCATTGCCGGAGGGAATGGAGAAACTGGAATCTTTGAGAAGCTTTTGCATCGGCGGGTGCCCTGAAGTAGAAGAACGATGCAAGCAGGGAGGGGAGGATTGGCCTAAGATTGCGCACATTCCTGATTTCGAGCACTTTCTTCTCGACAACTAA

mRNA sequence

ATGGCCGAAGCAATTCTCTTCGATGTTGCTGGGGCGATCTTGATGAAGCTAAGCTCTCAAGCTTTCCAGCGGCTTGGGATGCTATTTGGGCTGAAAGATGATCTTAACAAACTCACAGGCACAGTTTCAACCATTAAGGATGTGCTTCTTGATGCGGAGGGGCGTCAAACTAAAAGTCACTTGCTAGAGAATTGGCTCCAGAAATTGGAAGAAGCTCTTCATGATGCGGAGGATGTGCTTGATGAACTCTCTACGGAGGCTCTTCATCGAGAAGTGATGACTAGAGATAAAAATGCAAAACAAGTAAGGATCTTCTTCTCCAAATCTAATCAACTAGCACTTAATTATAGGATGGCTCGTCAAATAAAGAAAATTTGGGAAAGGCTAGATGCAATTGATGCTGAAAAAAAACAATTTCATTTGCATGAAAACTGTGAATCAAAGACTCGATACGGTTCATTTGATCAAATAATGACTGGAAGGGAATCTTGGTCTTTTTCAAATGAAGAGGAAGTGATTGGGAGGGATGATGATAAGAAAAAGGTAAAGGACCTTCTATTGGATGTAAATATGAATGTCACACACAATGTTTCATTCGTTGCCATAGTTGGGATGGGTGGAATAGGCAAGACAACCTTGGCTAAATCTCTCTACAATGATGAAAAATTATCAAAATATTTTGAGTTAAAAATATGGGTTTGGGTTTCTGATCAATTTGAGATAAAAGCGGTAGTGGAGAAAATAATAGAATCTGCAACCAAAAGCAATCCTCATGTAAAAGGAATGGAAGCTTTACAAACAGAGCTTCAAGAAGTGATTGGGGGAAAGAAGTATTTGCTAGTTATGGACGATGTATGGAATGAAAGTGAAGAGAAATGGCATGGGTTGAAACCTTTGTTAATGAGTGGTGCGAAAGGGAGTAAGATTTTGATCACAAAGCGTGATAGCAAAGTAGCTGCAGAAATTGAAAGCATGACTTCTTTATTCACTTTAGAAGGCTTACCGGATGGTAAATCTTGGTCATTATTTAGTAAAGTGGCATTTAAAGAAGGCAAAGAGGTTGAAAATCCAAACTTGATACATTTAGGAAAAGAAATTTTAGTGAAATCTGGGGGTGTTCCTCTTGTAATAAGACATATTGGCCGCTTGCTATACTCAAAAACTTCAGAAGAAGAATGGATGTCTTTCAAGAATAATGAACTTTTAGAGATCACTCAGCAAGACAATGATATGACATCGGTATTAAAATTGAGCTATAACCATCTCCCACCAAATCTGAAGCAGTGTTTTGCCTATTTATCCTTGTTTCCCAAAGGGAAGAGCTTAAAAATAAATGATTTGATTAGACAATGGATGGCTCTAGGTTTTATAGAATCATCAAATGGAACTAAATGCATGGAAGATACGGGAAATGACTATTTCAAGGAATTGTGTTGGAGGTTTTTTTTGGAAAATTCCATCAATGAGTGTAACTTCGACAATGATGTCCACGTGCACGATGTGATGCGTGATCTTGCAACAAATGTAGCAGGAAAGAAATATGTATGTGAAAATCTCAATCGTGATTATGCTTTCAATGAAAAAACTCGCCATGTTTCATTTGATTGTAAAATAAAATCATGGTCAGATGTTCTATCCAAATTACGCACGGCAAAAGGATTAAGAACGTTCCAACTATTCTCATCCAATTTTGAATATGAAAAGAAGAATGAGATCAATGAAGCTATTTTGGATGAAGTATTTTCCAGTTTTCCACGTTTGCGAGTATTGGGTCTTAATAACTCAAATAGTCGCACAGTGCCAAATTCTATAAGAAGGCTTAGACATCTTCGATATCTAGACCTCTCTGAAAATAATATGGAATCACTTCCAAATTCTATCACTGAATTGCAAAACTTGCAAACTCTAAATCTAATGTACTGTAGAGAACTAAAGGAATTGCCCAGGGACACCAGAAATCTTGTTAATCTTAGGCATCTTGATTTCAATACTTCTACCTTAACCCATATGCCAGAAGGGATAGGGAGGTTGAGTTGTCTACAGACACTAAGTTATTTTGTGTTGGACTGCAAAAGGTCTAGTCAGCTGAGTGAATTGAATGTGCTGATCCATTTAAATGGAGATCTAAGAATTATTGGTTTAGAGTATTTGAGGCATAATCCATCTGAAGTCAGCTTAGTAAACCTGAAAGACATAAAAGGCTTGAAGAATCTGCAACTGGAATGGAAACTGAGCCGAGATGATCAAGAATATGAAGGTGAGGATGATGAAACTGCAATAATGGAAGGCCTAGAACCACATCCAAATGTTGAATCCTTGCGCATTGACGGGTACAGTGGAGTAGGATTAACCAATTGGGTGTCCAGCTCCCTCTCAAAGTTAACTAAAATTATAATTTATAAATGTCATAGATTGCAACATCTAACTCAAATGGGCCATCTTCAGGCTCTCACATTTCTATATTTGGATGACATGAGATCTCTCAAGTTCATAGACAAGAATGAACCATCCTCCTCATCCTCTTTCCCATCTCTCGAGCTTCTAATTATCGCAAACATGCCTAATTTGGAAGGATGGTGGGAATTAGGGGAGACTCAAAAAAATTGGTTGCCTCCAACTTTTCCTAAGCTTATTTCCCTGCATATCTCTGGGTGTCCTAAGTTAAGTTTGATGCCCAAGCCAGCTTCCACTGGAGCAGTTGTGTTTTTATGTGATGTGGGTGTTCAATTGGTGAGTGCATTAGGTCCATTGTGGGGTTTGAAGAGTCTAACACTGGAGGAAATCGAGGATCTCAAATATCTAGAAATCATGCAAAACCTCGGTTCTTTGCCTATACAACTTCGACATTTGAAAATAAGGAAATGCTCAAATTTGATGAGTTTACCTGAATGGATTGGCATCCTCACTTCGCTTGAGGACCTGGAGATTATGGAATGTCCGAAATTAAAATCATTGCCGGAGGGAATGGAGAAACTGGAATCTTTGAGAAGCTTTTGCATCGGCGGGTGCCCTGAAGTAGAAGAACGATGCAAGCAGGGAGGGGAGGATTGGCCTAAGATTGCGCACATTCCTGATTTCGAGCACTTTCTTCTCGACAACTAA

Coding sequence (CDS)

ATGGCCGAAGCAATTCTCTTCGATGTTGCTGGGGCGATCTTGATGAAGCTAAGCTCTCAAGCTTTCCAGCGGCTTGGGATGCTATTTGGGCTGAAAGATGATCTTAACAAACTCACAGGCACAGTTTCAACCATTAAGGATGTGCTTCTTGATGCGGAGGGGCGTCAAACTAAAAGTCACTTGCTAGAGAATTGGCTCCAGAAATTGGAAGAAGCTCTTCATGATGCGGAGGATGTGCTTGATGAACTCTCTACGGAGGCTCTTCATCGAGAAGTGATGACTAGAGATAAAAATGCAAAACAAGTAAGGATCTTCTTCTCCAAATCTAATCAACTAGCACTTAATTATAGGATGGCTCGTCAAATAAAGAAAATTTGGGAAAGGCTAGATGCAATTGATGCTGAAAAAAAACAATTTCATTTGCATGAAAACTGTGAATCAAAGACTCGATACGGTTCATTTGATCAAATAATGACTGGAAGGGAATCTTGGTCTTTTTCAAATGAAGAGGAAGTGATTGGGAGGGATGATGATAAGAAAAAGGTAAAGGACCTTCTATTGGATGTAAATATGAATGTCACACACAATGTTTCATTCGTTGCCATAGTTGGGATGGGTGGAATAGGCAAGACAACCTTGGCTAAATCTCTCTACAATGATGAAAAATTATCAAAATATTTTGAGTTAAAAATATGGGTTTGGGTTTCTGATCAATTTGAGATAAAAGCGGTAGTGGAGAAAATAATAGAATCTGCAACCAAAAGCAATCCTCATGTAAAAGGAATGGAAGCTTTACAAACAGAGCTTCAAGAAGTGATTGGGGGAAAGAAGTATTTGCTAGTTATGGACGATGTATGGAATGAAAGTGAAGAGAAATGGCATGGGTTGAAACCTTTGTTAATGAGTGGTGCGAAAGGGAGTAAGATTTTGATCACAAAGCGTGATAGCAAAGTAGCTGCAGAAATTGAAAGCATGACTTCTTTATTCACTTTAGAAGGCTTACCGGATGGTAAATCTTGGTCATTATTTAGTAAAGTGGCATTTAAAGAAGGCAAAGAGGTTGAAAATCCAAACTTGATACATTTAGGAAAAGAAATTTTAGTGAAATCTGGGGGTGTTCCTCTTGTAATAAGACATATTGGCCGCTTGCTATACTCAAAAACTTCAGAAGAAGAATGGATGTCTTTCAAGAATAATGAACTTTTAGAGATCACTCAGCAAGACAATGATATGACATCGGTATTAAAATTGAGCTATAACCATCTCCCACCAAATCTGAAGCAGTGTTTTGCCTATTTATCCTTGTTTCCCAAAGGGAAGAGCTTAAAAATAAATGATTTGATTAGACAATGGATGGCTCTAGGTTTTATAGAATCATCAAATGGAACTAAATGCATGGAAGATACGGGAAATGACTATTTCAAGGAATTGTGTTGGAGGTTTTTTTTGGAAAATTCCATCAATGAGTGTAACTTCGACAATGATGTCCACGTGCACGATGTGATGCGTGATCTTGCAACAAATGTAGCAGGAAAGAAATATGTATGTGAAAATCTCAATCGTGATTATGCTTTCAATGAAAAAACTCGCCATGTTTCATTTGATTGTAAAATAAAATCATGGTCAGATGTTCTATCCAAATTACGCACGGCAAAAGGATTAAGAACGTTCCAACTATTCTCATCCAATTTTGAATATGAAAAGAAGAATGAGATCAATGAAGCTATTTTGGATGAAGTATTTTCCAGTTTTCCACGTTTGCGAGTATTGGGTCTTAATAACTCAAATAGTCGCACAGTGCCAAATTCTATAAGAAGGCTTAGACATCTTCGATATCTAGACCTCTCTGAAAATAATATGGAATCACTTCCAAATTCTATCACTGAATTGCAAAACTTGCAAACTCTAAATCTAATGTACTGTAGAGAACTAAAGGAATTGCCCAGGGACACCAGAAATCTTGTTAATCTTAGGCATCTTGATTTCAATACTTCTACCTTAACCCATATGCCAGAAGGGATAGGGAGGTTGAGTTGTCTACAGACACTAAGTTATTTTGTGTTGGACTGCAAAAGGTCTAGTCAGCTGAGTGAATTGAATGTGCTGATCCATTTAAATGGAGATCTAAGAATTATTGGTTTAGAGTATTTGAGGCATAATCCATCTGAAGTCAGCTTAGTAAACCTGAAAGACATAAAAGGCTTGAAGAATCTGCAACTGGAATGGAAACTGAGCCGAGATGATCAAGAATATGAAGGTGAGGATGATGAAACTGCAATAATGGAAGGCCTAGAACCACATCCAAATGTTGAATCCTTGCGCATTGACGGGTACAGTGGAGTAGGATTAACCAATTGGGTGTCCAGCTCCCTCTCAAAGTTAACTAAAATTATAATTTATAAATGTCATAGATTGCAACATCTAACTCAAATGGGCCATCTTCAGGCTCTCACATTTCTATATTTGGATGACATGAGATCTCTCAAGTTCATAGACAAGAATGAACCATCCTCCTCATCCTCTTTCCCATCTCTCGAGCTTCTAATTATCGCAAACATGCCTAATTTGGAAGGATGGTGGGAATTAGGGGAGACTCAAAAAAATTGGTTGCCTCCAACTTTTCCTAAGCTTATTTCCCTGCATATCTCTGGGTGTCCTAAGTTAAGTTTGATGCCCAAGCCAGCTTCCACTGGAGCAGTTGTGTTTTTATGTGATGTGGGTGTTCAATTGGTGAGTGCATTAGGTCCATTGTGGGGTTTGAAGAGTCTAACACTGGAGGAAATCGAGGATCTCAAATATCTAGAAATCATGCAAAACCTCGGTTCTTTGCCTATACAACTTCGACATTTGAAAATAAGGAAATGCTCAAATTTGATGAGTTTACCTGAATGGATTGGCATCCTCACTTCGCTTGAGGACCTGGAGATTATGGAATGTCCGAAATTAAAATCATTGCCGGAGGGAATGGAGAAACTGGAATCTTTGAGAAGCTTTTGCATCGGCGGGTGCCCTGAAGTAGAAGAACGATGCAAGCAGGGAGGGGAGGATTGGCCTAAGATTGCGCACATTCCTGATTTCGAGCACTTTCTTCTCGACAACTAA

Protein sequence

MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSHLLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMARQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDKNEPSSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMPKPASTGAVVFLCDVGVQLVSALGPLWGLKSLTLEEIEDLKYLEIMQNLGSLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSFCIGGCPEVEERCKQGGEDWPKIAHIPDFEHFLLDN
Homology
BLAST of Lsi02G025580 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.6e-152
Identity = 371/1062 (34.93%), Postives = 564/1062 (53.11%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
            MAEA L      +L  L+S    +L ++FG + +  KL+   STI+ VL DA+ +Q K  
Sbjct: 1    MAEAFL----QVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDK 60

Query: 61   LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
             +ENWLQKL  A ++ +D+L E   EA+  E         Q R+ F     +   +++ R
Sbjct: 61   AIENWLQKLNSAAYEVDDILGECKNEAIRFE---------QSRLGFYHPGIINFRHKIGR 120

Query: 121  QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
            ++K+I E+LDAI  E+++FH  E    +            RE+     E +V GRD ++ 
Sbjct: 121  RMKEIMEKLDAISEERRKFHFLEKITER------QAAAATRETGFVLTEPKVYGRDKEED 180

Query: 181  KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
            ++  +L++ N+NV   +    I+GMGG+GKTTLA+ ++NDE+++K+F  KIWV VSD F+
Sbjct: 181  EIVKILIN-NVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFD 240

Query: 241  IKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
             K +++ II +  +S+PHV+ + + Q +LQE++ GK+YLLV+DDVWN+  EKW  L+ +L
Sbjct: 241  EKRLIKTIIGNIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVL 300

Query: 301  MSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLI 360
              GA+G+ IL T R  KV + I      + L  L    S  LF + AF + KE  NPNL+
Sbjct: 301  TVGARGASILATTRLEKVGS-IMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEA-NPNLV 360

Query: 361  HLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYN 420
             +GKEI+ K GGVPL  + +G LL  K  E EW   ++NE+  + Q ++ +   L+LSY+
Sbjct: 361  AIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESSILPALRLSYH 420

Query: 421  HLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWR 480
            HLP +L+QCFAY ++FPK   +   +LI  WMA GF+  S G   +ED GN+ + EL  R
Sbjct: 421  HLPLDLRQCFAYCAVFPKDTKMIKENLITLWMAHGFL-LSKGNLELEDVGNEVWNELYLR 480

Query: 481  FFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLN----RDYAFNEKTRHVSFDC 540
             F +  I   + +    +HD++ DLAT++      C N+     +DY   + T  + F  
Sbjct: 481  SFFQ-EIEAKSGNTYFKIHDLIHDLATSLFSASASCGNIREINVKDY---KHTVSIGFAA 540

Query: 541  KIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSN 600
             + S+S  L K                                    F  LRVL L+ S 
Sbjct: 541  VVSSYSPSLLK-----------------------------------KFVSLRVLNLSYSK 600

Query: 601  SRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVN 660
               +P+SI  L HLRYLDLS NN  SLP  + +LQNLQTL++  C  L  LP+ T  L +
Sbjct: 601  LEQLPSSIGDLLHLRYLDLSCNNFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSS 660

Query: 661  LRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEY 720
            LRHL  +   LT  P  IG L+CL+TL +F++  K+  QL EL  L +L G + I  LE 
Sbjct: 661  LRHLVVDGCPLTSTPPRIGLLTCLKTLGFFIVGSKKGYQLGELKNL-NLCGSISITHLER 720

Query: 721  LRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRID 780
            ++++    +  NL     L++L + W    +D     E  E  ++E L+PHPN++ L I 
Sbjct: 721  VKNDTD--AEANLSAKANLQSLSMSWD---NDGPNRYESKEVKVLEALKPHPNLKYLEII 780

Query: 781  GYSGVGLTNWVSSS-LSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDD-MRSLKFIDKN 840
             + G    +W++ S L K+  + I  C     L   G L  L  L L +    +++++++
Sbjct: 781  AFGGFRFPSWINHSVLEKVISVRIKSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEED 840

Query: 841  EP----SSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSL 900
            +     S+  SFPSL+ L I    +L+G  +    +K      FP L  + I  CP L +
Sbjct: 841  DVHSRFSTRRSFPSLKKLRIWFFRSLKGLMKEEGEEK------FPMLEEMAILYCP-LFV 900

Query: 901  MPKPASTGAVVFLCDVGVQLVSALGPLWGLKSL-----------------TLEEIEDLKY 960
             P  +S   +    +   + +S++  L  L SL                 +L  +E L +
Sbjct: 901  FPTLSSVKKLEVHGNTNTRGLSSISNLSTLTSLRIGANYRATSLPEEMFTSLTNLEFLSF 960

Query: 961  LEIMQNLGSLPIQ------LRHLKIRKCSNLMSLPE-WIGILTSLEDLEIMECPKLKSLP 1020
             +  +NL  LP        L+ L+I  C +L S PE  +  LTSL  L +  C  LK LP
Sbjct: 961  FD-FKNLKDLPTSLTSLNALKRLQIESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLP 986

Query: 1021 EGMEKLESLRSFCIGGCPEVEERC-KQGGEDWPKIAHIPDFE 1028
            EG++ L +L +  + GCPEVE+RC K+ GEDW KIAHIP+ +
Sbjct: 1021 EGLQHLTALTNLGVSGCPEVEKRCDKEIGEDWHKIAHIPNLD 986

BLAST of Lsi02G025580 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 529.3 bits (1362), Expect = 1.0e-148
Identity = 364/1056 (34.47%), Postives = 566/1056 (53.60%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
            MAEA L      +L  L+      LG++FG + +  KL+   S I+ VL DA+ +Q K  
Sbjct: 1    MAEAFL----QVLLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYK 60

Query: 61   LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
             ++NWLQKL  A ++ +D+LD+  TEA            KQ  +       +   Y++ +
Sbjct: 61   AIKNWLQKLNVAAYEVDDILDDCKTEA---------ARFKQAVLGRYHPRTITFCYKVGK 120

Query: 121  QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
            ++K++ E+LDAI  E++ FHL E           ++    R++     E +V GR+ ++ 
Sbjct: 121  RMKEMMEKLDAIAEERRNFHLDERI--------IERQAARRQTGFVLTEPKVYGREKEED 180

Query: 181  KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
            ++  +L++ N++ +  V  + I+GMGG+GKTTLA+ ++ND++++++F LKIWV VSD F+
Sbjct: 181  EIVKILIN-NVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFD 240

Query: 241  IKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
             K +++ I+ES    +     +  LQ +LQE++ GK+Y LV+DDVWNE +EKW  L+ +L
Sbjct: 241  EKRLIKAIVESIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVL 300

Query: 301  MSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLI 360
              GA G+ ILIT R  K+ + I     L+ L  L     W LF + AF    E  +P L+
Sbjct: 301  KIGASGASILITTRLEKIGS-IMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTET-SPKLM 360

Query: 361  HLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYN 420
             +GKEI+ K GGVPL  + +G LL  K  E EW   +++E+  + Q +N +   L+LSY+
Sbjct: 361  EIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDENSVLPALRLSYH 420

Query: 421  HLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWR 480
            HLP +L+QCFAY ++FPK   ++   LI  WMA  F+  S G   +ED GN+ + EL  R
Sbjct: 421  HLPLDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFL-LSKGNMELEDVGNEVWNELYLR 480

Query: 481  FFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKS 540
             F +  I   +      +HD++ DLAT++             ++ +  +R +     +K 
Sbjct: 481  SFFQ-EIEVKSGKTYFKMHDLIHDLATSM-------------FSASASSRSIR-QINVKD 540

Query: 541  WSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTV 600
              D++  +   K      + S  F     +E+  +    +F  F  LRVL L+NS    +
Sbjct: 541  DEDMMFIVTNYK-----DMMSIGF-----SEVVSSYSPSLFKRFVSLRVLNLSNSEFEQL 600

Query: 601  PNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRHL 660
            P+S+  L HLRYLDLS N + SLP  + +LQNLQTL+L  C+ L  LP+ T  L +LR+L
Sbjct: 601  PSSVGDLVHLRYLDLSGNKICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNL 660

Query: 661  DFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYLRHN 720
              +   LT MP  IG L+CL+TL YFV+  ++  QL EL  L +L G + I  LE ++ N
Sbjct: 661  VLDHCPLTSMPPRIGLLTCLKTLGYFVVGERKGYQLGELRNL-NLRGAISITHLERVK-N 720

Query: 721  PSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDGYSG 780
              E    NL     L +L + W     D+    E +E  ++E L+PHPN++ L I  + G
Sbjct: 721  DMEAKEANLSAKANLHSLSMSW-----DRPNRYESEEVKVLEALKPHPNLKYLEIIDFCG 780

Query: 781  VGLTNWVSSS-LSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDD-MRSLKFIDKNEPSS 840
              L +W++ S L  +  I+I  C     L   G L  L  L L D    +++++ +   +
Sbjct: 781  FCLPDWMNHSVLKNVVSILISGCENCSCLPPFGELPCLESLELQDGSVEVEYVEDSGFLT 840

Query: 841  SSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMPKPASTG 900
               FPSL  L I    NL+G   +   ++      FP L  + IS CP + + P  +S  
Sbjct: 841  RRRFPSLRKLHIGGFCNLKGLQRMKGAEQ------FPVLEEMKISDCP-MFVFPTLSSVK 900

Query: 901  AVVFLCDV---GVQLVSALGPLWGLKSLT-------LEE----IEDLKYLEI--MQNLGS 960
             +    +    G+  +S L  L  LK  +       LEE    +E+L YL +  ++NL  
Sbjct: 901  KLEIWGEADAGGLSSISNLSTLTSLKIFSNHTVTSLLEEMFKNLENLIYLSVSFLENLKE 960

Query: 961  LPI------QLRHLKIRKCSNLMSLP-EWIGILTSLEDLEIMECPKLKSLPEGMEKLESL 1020
            LP        L+ L IR C  L SLP E +  L+SL +L +  C  LK LPEG++ L +L
Sbjct: 961  LPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTL 992

Query: 1021 RSFCIGGCPEVEERCKQG-GEDWPKIAHIPDFEHFL 1031
             S  I GCP++ +RC++G GEDW KI+HIP+   ++
Sbjct: 1021 TSLKIRGCPQLIKRCEKGIGEDWHKISHIPNVNIYI 992

BLAST of Lsi02G025580 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.3e-140
Identity = 367/1046 (35.09%), Postives = 565/1046 (54.02%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
            MAEA +      +L  L+S     L +LFG +D+  +L+   STI+ VL DA+ +Q  + 
Sbjct: 1    MAEAFI----QVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNK 60

Query: 61   LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
             LENWLQKL  A ++ +D+LDE  T+A      TR   ++  R        +   +++ +
Sbjct: 61   PLENWLQKLNAATYEVDDILDEYKTKA------TRFSQSEYGRY---HPKVIPFRHKVGK 120

Query: 121  QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
            ++ ++ ++L AI  E+K FHLHE           ++    RE+ S   E +V GRD +K 
Sbjct: 121  RMDQVMKKLKAIAEERKNFHLHEKI--------VERQAVRRETGSVLTEPQVYGRDKEKD 180

Query: 181  KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
            ++  +L++ N++   ++S + I+GMGG+GKTTLA+ ++ND++++++F  KIW+ VS+ F+
Sbjct: 181  EIVKILIN-NVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFD 240

Query: 241  IKAVVEKIIESATKSNPHVKGME--ALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKP 300
             K +++ I+ES  +  P +  M+   LQ +LQE++ GK+YLLV+DDVWNE ++KW  L+ 
Sbjct: 241  EKRLIKAIVES-IEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRA 300

Query: 301  LLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPN 360
            +L  GA G+ +L T R  KV + I      + L  L     W LF + AF   +E+ NPN
Sbjct: 301  VLKVGASGASVLTTTRLEKVGS-IMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEI-NPN 360

Query: 361  LIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLS 420
            L+ +GKEI+ KSGGVPL  + +G +L  K  E  W   +++ +  + Q ++ +   L+LS
Sbjct: 361  LVAIGKEIVKKSGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESSILPALRLS 420

Query: 421  YNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELC 480
            Y+ LP +LKQCFAY ++FPK   ++   LI  WMA GF+  S G   +ED G++ +KEL 
Sbjct: 421  YHQLPLDLKQCFAYCAVFPKDAKMEKEKLISLWMAHGFL-LSKGNMELEDVGDEVWKELY 480

Query: 481  WRFFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKI 540
             R F +  I   +      +HD++ DLAT++        N+       E  +H       
Sbjct: 481  LRSFFQ-EIEVKDGKTYFKMHDLIHDLATSLFSANTSSSNI------REINKH------- 540

Query: 541  KSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSR 600
             S++ ++S      G      F +    EK               F  LRVL L +S   
Sbjct: 541  -SYTHMMS-----IGFAEVVFFYTLPPLEK---------------FISLRVLNLGDSTFN 600

Query: 601  TVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLR 660
             +P+SI  L HLRYL+L  + M SLP  + +LQNLQTL+L YC +L  LP++T  L +LR
Sbjct: 601  KLPSSIGDLVHLRYLNLYGSGMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLR 660

Query: 661  HLDFNTS-TLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYL 720
            +L  + S +LT MP  IG L+CL+TL  FV+  K+  QL EL  L +L G ++I  LE +
Sbjct: 661  NLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQLGELGNL-NLYGSIKISHLERV 720

Query: 721  RHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDG 780
            + N  +    NL     L +L + W  +     YE E  E  ++E L+PH N+ SL+I G
Sbjct: 721  K-NDKDAKEANLSAKGNLHSLSMSWN-NFGPHIYESE--EVKVLEALKPHSNLTSLKIYG 780

Query: 781  YSGVGLTNWVSSS-LSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLD----DMRSLKFID 840
            + G+ L  W++ S L  +  I+I        L   G L  L  L L     D+  ++ +D
Sbjct: 781  FRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVD 840

Query: 841  KNEPSSSSS---FPSLELLIIANMPNLEGWWEL-GETQKNWLPPTFPKLISLHISGCPKL 900
             +  S   +   FPSL  L I +  +L+G  +  GE Q       FP L  + I  CP L
Sbjct: 841  IDVHSGFPTRIRFPSLRKLDIWDFGSLKGLLKKEGEEQ-------FPVLEEMIIHECPFL 900

Query: 901  SLMPK-PASTGAVVFLCDVGVQLVSAL-GPLWGLKSLTLEEIEDLKYLEIMQNLGSLPIQ 960
            +L     A T   +    V       +   L  LK LT+    +LK  E+  +L SL   
Sbjct: 901  TLSSNLRALTSLRICYNKVATSFPEEMFKNLANLKYLTISRCNNLK--ELPTSLASLN-A 960

Query: 961  LRHLKIRKCSNLMSLP-EWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSFCIGGCPE 1020
            L+ LKI+ C  L SLP E +  L+SL +L +  C  LK LPEG++ L +L S  I GCP+
Sbjct: 961  LKSLKIQLCCALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQ 970

Query: 1021 VEERCKQG-GEDWPKIAHIPDFEHFL 1031
            + +RC++G GEDW KI+HIP+   ++
Sbjct: 1021 LIKRCEKGIGEDWHKISHIPNVNIYI 970

BLAST of Lsi02G025580 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 492.7 bits (1267), Expect = 1.0e-137
Identity = 355/1051 (33.78%), Postives = 551/1051 (52.43%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
            MAEA +      +L  L+S     L +LFG +D+  +L+   STI+ VL DA+ +Q    
Sbjct: 1    MAEAFI----QVVLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDK 60

Query: 61   LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
             LENWLQKL  A ++ +D+LDE  T+A      TR   ++  R        +   +++ +
Sbjct: 61   PLENWLQKLNAATYEVDDILDEYKTKA------TRFLQSEYGRY---HPKVIPFRHKVGK 120

Query: 121  QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
            ++ ++ ++L+AI  E+K+FHL E           ++    RE+ S   E +V GRD +K 
Sbjct: 121  RMDQVMKKLNAIAEERKKFHLQEKI--------IERQAATRETGSVLTEPQVYGRDKEKD 180

Query: 181  KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
            ++  +L++   +    +S + I+GMGG+GKTTL++ ++ND+++++ F  KIW+ +SD F 
Sbjct: 181  EIVKILINTASD-AQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFN 240

Query: 241  IKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
             K +++ I+ES    +     +  LQ +LQE++ GK+Y LV+DDVWNE + KW  L+ +L
Sbjct: 241  EKRLIKAIVESIEGKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVL 300

Query: 301  MSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLI 360
              GA G+ +L T R  KV + I      + L  L     W LF + AF   +E+ NPNL+
Sbjct: 301  KVGASGAFVLTTTRLEKVGS-IMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEI-NPNLM 360

Query: 361  HLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYN 420
             +GKEI+ K GGVPL  + +G +L  K  E EW   +++ +  + Q ++ +   L+LSY+
Sbjct: 361  AIGKEIVKKCGGVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSILPALRLSYH 420

Query: 421  HLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWR 480
            HLP +L+QCF Y ++FPK   +   +LI  WMA GF+  S G   +ED GN+ + EL  R
Sbjct: 421  HLPLDLRQCFVYCAVFPKDTKMAKENLIAFWMAHGFL-LSKGNLELEDVGNEVWNELYLR 480

Query: 481  FFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKS 540
             F +  I   +      +HD++ DLAT++                        F     S
Sbjct: 481  SFFQ-EIEVESGKTYFKMHDLIHDLATSL------------------------FSANTSS 540

Query: 541  WSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTV 600
                 S +R         + S  F      E+  +    +   F  LRVL L NSN   +
Sbjct: 541  -----SNIREINANYDGYMMSIGFA-----EVVSSYSPSLLQKFVSLRVLNLRNSNLNQL 600

Query: 601  PNSIRRLRHLRYLDLSEN-NMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRH 660
            P+SI  L HLRYLDLS N  + +LP  + +LQNLQTL+L YC  L  LP+ T  L +LR+
Sbjct: 601  PSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRN 660

Query: 661  LDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYLRH 720
            L  +  +LT  P  IG L+CL++LS FV+  ++  QL EL  L +L G + I  L+ ++ 
Sbjct: 661  LLLDGCSLTSTPPRIGLLTCLKSLSCFVIGKRKGHQLGELKNL-NLYGSISITKLDRVKK 720

Query: 721  NPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDGYS 780
            + ++    NL     L +L L W L      Y+ E     ++E L+PH N++ L I+G+ 
Sbjct: 721  D-TDAKEANLSAKANLHSLCLSWDLD-GKHRYDSE-----VLEALKPHSNLKYLEINGFG 780

Query: 781  GVGLTNWVSSS-LSKLTKIIIYKCHRLQHLTQMGHLQALTFLYL-DDMRSLKFIDKNEPS 840
            G+ L +W++ S L  +  I I  C     L   G L  L  L L      +++++ N   
Sbjct: 781  GIRLPDWMNQSVLKNVVSIRIRGCENCSCLPPFGELPCLESLELHTGSADVEYVEDN--V 840

Query: 841  SSSSFPSLELLIIANMPNLEGWWEL-GETQKNWLPPTFPKLISLHISGCPKLSLMPKPAS 900
                FPSL  L+I +  NL+G  ++ GE Q       FP L  +    CP + ++P  +S
Sbjct: 841  HPGRFPSLRKLVIWDFSNLKGLLKMEGEKQ-------FPVLEEMTFYWCP-MFVIPTLSS 900

Query: 901  --------TGAVVFLCDVGVQLVSALGPLWGLKSLTLEE-----IEDLKYLEI--MQNLG 960
                    T A V      ++ +++L     +++ +L E     + +LKYL+I   +NL 
Sbjct: 901  VKTLKVIVTDATVLRSISNLRALTSLDISDNVEATSLPEEMFKSLANLKYLKISFFRNLK 960

Query: 961  SLPIQ------LRHLKIRKCSNLMSLP-EWIGILTSLEDLEIMECPKLKSLPEGMEKLES 1020
             LP        L+ LK   C  L SLP E +  LTSL +L +  C  LK LPEG++ L +
Sbjct: 961  ELPTSLASLNALKSLKFEFCDALESLPEEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTA 973

Query: 1021 LRSFCIGGCPEVEERCKQG-GEDWPKIAHIP 1025
            L +  I  CP V +RC++G GEDW KIAHIP
Sbjct: 1021 LTTLTITQCPIVFKRCERGIGEDWHKIAHIP 973

BLAST of Lsi02G025580 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.3e-119
Identity = 313/1029 (30.42%), Postives = 518/1029 (50.34%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDD-LNKLTGTVSTIKDVLLDAEGRQTKS 60
            + E  L     A+   L S+ F+       L ++ L +L+  + TI  VL+DAE +Q  +
Sbjct: 4    IGEMFLAAFLQALFQTLVSEPFRSFFKRRELNENLLERLSTALLTITAVLIDAEEKQITN 63

Query: 61   HLLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNA---KQVRIFFSKSNQLALN- 120
             ++E W+ +L + ++ AED LD+++TEAL   +     ++   +Q+R   S  + L  N 
Sbjct: 64   PVVEKWVNELRDVVYHAEDALDDIATEALRLNIGAESSSSNRLRQLRGRMSLGDFLDGNS 123

Query: 121  YRMARQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGR 180
              +  +++K+  RL+ + +++    L E     T      ++ T     S  +E EV GR
Sbjct: 124  EHLETRLEKVTIRLERLASQRNILGLKE----LTAMIPKQRLPT----TSLVDESEVFGR 183

Query: 181  DDDKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWV 240
            DDDK ++   L+  N    + ++ VAIVG+GG+GKTTL++ LYND+ +  YF  K+W  V
Sbjct: 184  DDDKDEIMRFLIPEN-GKDNGITVVAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHV 243

Query: 241  SDQFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGK--KYLLVMDDVWNESEEKW 300
            S++F++  + +K+ ES T        ++ LQ +L+E + G    +LLV+DD+WNE+   W
Sbjct: 244  SEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLTGTGLPFLLVLDDLWNENFADW 303

Query: 301  HGLKPLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKE 360
              L+   +  A+GS+IL+T R  +VA+ I     +  L+ L DG  WSLF K  F   + 
Sbjct: 304  DLLRQPFIHAAQGSQILVTTRSQRVAS-IMCAVHVHNLQPLSDGDCWSLFMKTVFGNQEP 363

Query: 361  VENPNLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTS 420
              N  +  L + I+ K  G+PL ++ +G +L  +    EW    ++ + ++    +++  
Sbjct: 364  CLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADKSNLLP 423

Query: 421  VLKLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDY 480
            VL++SY +LP +LK+CFAY S+FPKG + + + ++  WMA GF++ +  +K +E+ GN+Y
Sbjct: 424  VLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEY 483

Query: 481  FKELCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVS 540
            F EL  R  L+ +           +HD + +LA   +G+             +E+TR++S
Sbjct: 484  FSELESRSLLQKT------KTRYIMHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLS 543

Query: 541  FDCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLN 600
            +     +       LR  K LRTF L  S     +   +++ + +++  +  RLRVL L+
Sbjct: 544  YLRDNYAEPMEFEALREVKFLRTF-LPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLS 603

Query: 601  NSN-SRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTR 660
            +   +R  P+  + + H R+LDLS   +E LP S+  + NLQTL L YC  LKELP D  
Sbjct: 604  HYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDIS 663

Query: 661  NLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRII 720
            NL+NLR+LD   + L  MP   GRL  LQTL+ F +     S++SEL  L  L+G L+I+
Sbjct: 664  NLINLRYLDLIGTKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIV 723

Query: 721  GLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGE----DDETAIMEGLEPHP 780
             L+ +  + ++ +  NL   K L+ +   W+      E         +E  + E L PH 
Sbjct: 724  ELQRV-VDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHR 783

Query: 781  NVESLRIDGYSGVGLTNWVSS-SLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRS 840
            ++E L I+ Y G    +W+S  S S++  I + +C     L  +G L  L  L++  M  
Sbjct: 784  HIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVG 843

Query: 841  LKFIDKN--------EPSSSSSFPSLELLIIANMPNLEGWWELGETQKNWLP-------- 900
            L+ I +                F SLE L   N+P+ + W ++  T+ +  P        
Sbjct: 844  LQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFIL 903

Query: 901  ---------PTF-PKLISLHISGCPKLSLMP-------KPASTGAVVFLCDVGVQLVSAL 960
                     PTF P LISLHI  C  L   P       +   T ++   CD  V+    L
Sbjct: 904  RCPELTGTLPTFLPSLISLHIYKCGLLDFQPDHHEYSYRNLQTLSIKSSCDTLVKF--PL 963

Query: 961  GPLWGLKSLTLEEIEDLKYLEIMQNLGSLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDL 984
                 L  L +++   L  LE+       P  LR+L+I  C NL  LP+   +  +L+ +
Sbjct: 964  NHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLPKLNALPQNLQ-V 1011

BLAST of Lsi02G025580 vs. ExPASy TrEMBL
Match: A0A6J1D168 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111016078 PE=4 SV=1)

HSP 1 Score: 1351.3 bits (3496), Expect = 0.0e+00
Identity = 720/1053 (68.38%), Postives = 836/1053 (79.39%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGR-QTKS 60
            MAE +LF VAG ILMKL S A QRLGM+ G+++DL KLT TVS IK+VLLDAE   QTK+
Sbjct: 1    MAEGVLFHVAGEILMKLGSIASQRLGMVRGVENDLTKLTSTVSVIKNVLLDAEEHCQTKT 60

Query: 61   -HLLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRM 120
             H L++WLQKLEEAL+DAEDVLDELS+E LHREV+T+ KNAKQVRIFFS SNQ+A NYR+
Sbjct: 61   DHTLQDWLQKLEEALYDAEDVLDELSSETLHREVVTKGKNAKQVRIFFSGSNQIAFNYRV 120

Query: 121  ARQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGR-ESWSFSNEEEVIGRDD 180
            AR IKKIWERLDAIDAE+KQF+L ENC+ +T YGSFDQIM GR E+ SF N EEVIGRD 
Sbjct: 121  ARNIKKIWERLDAIDAERKQFNLTENCQQRTLYGSFDQIMMGRKETCSFPNVEEVIGRDY 180

Query: 181  DKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSD 240
            DKKK+KDLLLD+NMNV HNVSF+ I GMGGIGKTTLAKSLY+D ++S+ F+  IW+W+SD
Sbjct: 181  DKKKLKDLLLDINMNVRHNVSFIVIAGMGGIGKTTLAKSLYHDREVSECFDSTIWIWISD 240

Query: 241  QFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLK 300
            QFE++ VVEKIIESATK  P+V+GMEAL +ELQEVIGGKKYLLVMDDVW E+EEKWH LK
Sbjct: 241  QFEVRVVVEKIIESATKRKPNVEGMEALNSELQEVIGGKKYLLVMDDVWKETEEKWHELK 300

Query: 301  PLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENP 360
            PLLM GA GSKILITKRDSKVA EIESMTSLFTL+GL +  SWSLF KVAFKE K+  N 
Sbjct: 301  PLLMGGASGSKILITKRDSKVATEIESMTSLFTLKGLQESNSWSLFRKVAFKEAKDPANS 360

Query: 361  NLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQ---DNDMTSV 420
            NLI LGKEIL K GGVPLVIRHIGRLLYSKTSEEEWMSFKNNELL++ QQ   ++ +T +
Sbjct: 361  NLIKLGKEILAKCGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLKVIQQTDKNDGLTPI 420

Query: 421  LKLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGT-KCMEDTGNDY 480
            L LSYNHLPPNLKQCFAYLSLFPKG  L+I DLIRQWMA GFIESSNGT   ME+ G  Y
Sbjct: 421  LNLSYNHLPPNLKQCFAYLSLFPKGTRLEIKDLIRQWMAHGFIESSNGTANSMEEVGKSY 480

Query: 481  FKELCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVS 540
            FKELCWRFF ENS +ECNFD+DV +HDVMR++A NVAG+KYV  NLN D+  +EKTRH+ 
Sbjct: 481  FKELCWRFFFENSSDECNFDDDVDMHDVMREVAMNVAGEKYVHGNLNCDFVVSEKTRHIL 540

Query: 541  FDCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEIN-EAILDEVFSSFPRLRVLGL 600
            FD KIK W DVL KL+ AKGLRTF LF S F ++ + E N EA+L+E+ SSFPRLRVLGL
Sbjct: 541  FDSKIKLWQDVLLKLQKAKGLRTF-LFLSYFPFKNRLEDNIEAVLEELCSSFPRLRVLGL 600

Query: 601  NNSNSRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTR 660
            N+S    VPNSI+ LRHLRYL+LS N MESLP SIT+LQNLQ+L+L  C +L ELPRDT 
Sbjct: 601  NHSKIHVVPNSIKNLRHLRYLNLSRNYMESLPTSITKLQNLQSLDLKDCYKLNELPRDTS 660

Query: 661  NLVNLRHLDF--NTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLR 720
            NLVNLRHL    +  T  HM EG+ +LSCL+TL YFVLDCKRS+  SELNV  + NG L+
Sbjct: 661  NLVNLRHLVLPDHLDTPLHMLEGMRKLSCLRTLGYFVLDCKRSNMQSELNVPNYFNGRLK 720

Query: 721  IIGLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLS-RDDQEYEGEDDETAIMEGLEPHPN 780
            I+GLE LR  PS+V LVNLKDI+GL++L+L WKLS RDD E EG+ DE  +MEGL+P  N
Sbjct: 721  IVGLEQLRRTPSKVGLVNLKDIQGLRDLKLVWKLSGRDDDEGEGKGDE-RVMEGLQPQSN 780

Query: 781  VESLRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLK 840
            VESLRI+GYSGVGL NWVS+SLS LTKI I  CHR+QHL Q+ HLQ L  ++L+DMRSLK
Sbjct: 781  VESLRIEGYSGVGLPNWVSTSLSNLTKIAICNCHRVQHLPQLAHLQTLKGIFLEDMRSLK 840

Query: 841  FIDKNEPSSSSSFPSLELLIIANMPNLEGWWE---LGETQKNWLPPTFPKLISLHISGCP 900
            F+DKNEP     FPSL  L + NM NLEGWWE   +GET      P FP+L  L I  CP
Sbjct: 841  FVDKNEP-GLLCFPSLVSLRLKNMGNLEGWWESRVVGETL-----PIFPELQYLRIQHCP 900

Query: 901  KLSLMPKPA-STGAVVFLCDVGVQLVSALGPLWGLKSLTLEEIEDLKYLEI------MQN 960
            KLSLMPK A ST    FLCDVGVQ+V+A+GPLW  + + L+EI+DLK LE+       Q+
Sbjct: 901  KLSLMPKIASSTLDSFFLCDVGVQVVTAIGPLWNFEVVILQEIKDLKDLEMESHQNRNQD 960

Query: 961  LGS--------LPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEK 1020
            LGS         P +LR L I KCSNLMSLPEWIG +TSL  + I++CPKLKSL EGM +
Sbjct: 961  LGSSSLSMSSRSPTRLRFLTIEKCSNLMSLPEWIGTITSLWKVTIIDCPKLKSLLEGMRQ 1020

Query: 1021 LESLRSFCIGGCPEVEERCKQGGEDWPKIAHIP 1025
            L+SL+   I GCPE+EERCK+GGEDWP IAHIP
Sbjct: 1021 LKSLKYLTIKGCPELEERCKEGGEDWPNIAHIP 1045

BLAST of Lsi02G025580 vs. ExPASy TrEMBL
Match: A0A6J1GPK9 (putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111456321 PE=4 SV=1)

HSP 1 Score: 1151.0 bits (2976), Expect = 0.0e+00
Identity = 636/1111 (57.25%), Postives = 782/1111 (70.39%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
            MAE +LF++A  I+ KLSS A Q+LGML+G+KD+L +L  +VS IK V+LDAE RQTKSH
Sbjct: 1    MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEERQTKSH 60

Query: 61   LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
             L++WL+KL+EAL+DAEDVLDE+S EA+      R + AKQVRIFFS+ N +A +Y+M R
Sbjct: 61   FLKDWLEKLKEALYDAEDVLDEISGEAVR-----RGRKAKQVRIFFSRYNPIAFDYKMTR 120

Query: 121  QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
            QIK + ERL  I AE+K FHL E    KT++GSFDQI  G E+ SFSN EEVIGRDD K+
Sbjct: 121  QIKNVRERLGVIAAERKNFHLTEIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKR 180

Query: 181  KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
             +KDLL D  MNV  N+SF+AIVGM GIGKTTLA+SL+ND ++S+ F+LKIWVWVS  FE
Sbjct: 181  VIKDLLQD--MNVKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFE 240

Query: 241  IKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
            +K VVEKIIESAT S P+V GME+LQT+LQ+++ GKKYLLVMDD+WNESE+KW  LK LL
Sbjct: 241  VKIVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLL 300

Query: 301  MSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLI 360
            M GA+GSKI+ITKRDS +A EI +MTSL TL+GL +  SWSLF KVAF +G E ENP LI
Sbjct: 301  MGGARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLI 360

Query: 361  HLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYN 420
             LGKEILVK GGVPLVIRHIGRLLY + SEEEW+SFKNN+LLE+ Q+D+DM S+LKLSYN
Sbjct: 361  QLGKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEVIQRDHDMASILKLSYN 420

Query: 421  HLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWR 480
            HLP NLKQCFAY SL  K + +  ++LIRQW+A GF++S NG K MEDTGNDYF ELCWR
Sbjct: 421  HLPSNLKQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLNGCKSMEDTGNDYFMELCWR 480

Query: 481  FFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKS 540
            FF EN  +E NF++DV++HDVM DLA  VAG +YV  N N D+   E TRH+ FD +I  
Sbjct: 481  FFYENFNDEHNFEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVGESTRHILFDREIGI 540

Query: 541  WSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTV 600
            W  V SKLR AKGLRTF L  SN   ++KN++NE +LDE+F  F RLRVL L  SN + V
Sbjct: 541  WEHVPSKLRKAKGLRTFIL--SNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRV 600

Query: 601  PNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRHL 660
            P+SI+RL HLRYLDLS N M+SLPNS+T L NLQTLNL  C  LK+LPR TRNLVNLRHL
Sbjct: 601  PDSIKRLTHLRYLDLSGNYMKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRHL 660

Query: 661  DFNTST-LTHMPEGIGRLSCLQTLSYFVLDC---KRSSQLSELNVLIHLNGDLRIIGLEY 720
            + + ST LTHMPEG+G+L+CLQTLS FVLD    +  S+L ELN L  L G L IIGLE 
Sbjct: 661  NISDSTHLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLEN 720

Query: 721  LRHNPSEVSLVNLKDIKGLKNLQLEWK-LSRDDQEYEGEDDETAIMEGLEPHPNVESLRI 780
            L+  PSE SL NLK+ KGL++L L WK LS D+  ++ +++   I+ GLEP+P +ES RI
Sbjct: 721  LKFTPSEASLANLKEKKGLRHLTLIWKNLSGDEHRFQADEE---ILAGLEPNPAIESFRI 780

Query: 781  DGYSGVGLTNW--VSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDK 840
             GY GV L NW  +SS L KLT+I+I  C RLQ+L Q+ HL AL  LYL ++ SLKFID 
Sbjct: 781  SGYFGVKLPNWLSLSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKFIDN 840

Query: 841  NEP-SSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMP 900
             +P SS+  FPSLE L + +MPNLE WWE     +    PTFP L  L IS CPKL+ MP
Sbjct: 841  TDPYSSALFFPSLESLCLMDMPNLEAWWERESMVEEEFLPTFPCLTILSISNCPKLATMP 900

Query: 901  -KPASTGAVVFLCDVGVQLVSAL------GPLWGLKSLTLEEIEDLKYL--EIMQNLG-- 960
              P  T   + L  VG+Q++S++       P   LKSL L+ IEDLK L  ++ QNL   
Sbjct: 901  GHPPLTD--ITLDGVGLQMMSSIRSSSNTSPSSSLKSLELKGIEDLKNLSHKLRQNLNSL 960

Query: 961  --------------------------------------------------------SLPI 1020
                                                                    SLPI
Sbjct: 961  KFLYITECESLVNSLFDETRSSSTSTRNYTELGDAMQWKFLHNLKFLRLENLPKLVSLPI 1020

Query: 1021 QLRH------LKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSFC 1031
            +++H      L I KC N  SLPEWIG + SL++LEI+ECPKLKSLPEG+ +L++L    
Sbjct: 1021 EVQHWTALKFLIISKCPNFRSLPEWIGGMVSLQELEILECPKLKSLPEGIRELKALNHLR 1080

BLAST of Lsi02G025580 vs. ExPASy TrEMBL
Match: A0A1S3CEH4 (disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=1)

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 617/1057 (58.37%), Postives = 769/1057 (72.75%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
            MAE IL  +AG IL KL S   +  GML GLKDDL++L  TVS I+  L+DA+   T   
Sbjct: 255  MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNLPTGLD 314

Query: 61   LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
             +  WL+KL   LHDAED++DE   E  HREVMTR+K+AK+VRIFFS SNQLA NY+MAR
Sbjct: 315  SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 374

Query: 121  QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFD---QIMTGRESWSFSNEEEVIGRDD 180
            ++K+I ERLD ID EK     H   + + +        ++   RE+ S  N+E VIGRDD
Sbjct: 375  RVKRIRERLDVIDKEKSYLWSHSFVQGRQQDVPSPIRMRMSLSRETHSSLNKERVIGRDD 434

Query: 181  DKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSD 240
            DK+ +K LLL  +M++  NVS +AIVGMGGIGKTTLAKSLYND  +SK+FE+K+W+WVS 
Sbjct: 435  DKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSKHFEIKMWIWVSK 494

Query: 241  QFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLK 300
            QF  K VV+KI+ESATK  P  + ++ +Q++LQ+VIGGKKYLLVMD++ NESE++W  LK
Sbjct: 495  QFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKKLK 554

Query: 301  PLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEG-KEVEN 360
             LL+ G +GSKI+ITKRD KVA+EIE MT+  TL+GL + +SWSLF +VAF+       N
Sbjct: 555  DLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDRSWSLFREVAFEGNLPNPIN 614

Query: 361  PNLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLK 420
            P L  +GKEI  + GGVPL IRH+GR L SK  EEEWMS  +++LLE+TQQ+ND+ S+L+
Sbjct: 615  PKLELMGKEISSRCGGVPLAIRHMGRFLCSKRCEEEWMSSLSDKLLEVTQQNNDVASILE 674

Query: 421  LSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKE 480
            LSYN LP +LKQCFAY SLFPK   LK+N+LIRQW+A GFIESS G K ME  G +YF E
Sbjct: 675  LSYNDLPLHLKQCFAYSSLFPKVHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYFDE 734

Query: 481  LCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKK-YVCENLNRDYAFNEKTRHVSFD 540
            LCWRFF ENS +E NFD+ V++HDVM +LA  VA KK YVC ++N +Y  +E+T H SFD
Sbjct: 735  LCWRFFYENSSDENNFDDYVYMHDVMYELARKVADKKLYVCGDINNNYVVSEQTLHFSFD 794

Query: 541  CKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNS 600
             KI+SW DVLSKL  AKGLRTF    S   YE+KNE+NEA LDE+FSSFPRLRVL L  S
Sbjct: 795  YKIQSWQDVLSKLCKAKGLRTFMFLHS--PYEEKNEVNEANLDELFSSFPRLRVLLLGGS 854

Query: 601  NSRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLV 660
            N   +PNSI++L+ LRYLDLSENNM+SLPNSITELQ+LQTL L  C  LKE+PRD   LV
Sbjct: 855  NIHVLPNSIKKLKLLRYLDLSENNMKSLPNSITELQDLQTLKLTGCYALKEMPRDINKLV 914

Query: 661  NLRHLDFN-TSTLTHM-PEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIG 720
            NLRHLD    S +THM PEG+ +LSCLQT+S FV DC ++++L ELN L +L G+L+I G
Sbjct: 915  NLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFVFDCTKTNKLRELNELNNLTGELKITG 974

Query: 721  LEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESL 780
            L+ LR  P EVSLVNLKD K L++L+LEWK+     ++E E DE  +M+GLEP+PNVESL
Sbjct: 975  LQQLRSTPYEVSLVNLKDKKCLRSLKLEWKMGL--YKHEDEADE-RVMKGLEPNPNVESL 1034

Query: 781  RIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDK 840
             I GY+GVGL  W+ +   KLT+I I  CHRLQHL Q  HLQ L  L L D+RSLKFIDK
Sbjct: 1035 GIKGYNGVGLPKWMFNCHLKLTEIEIVSCHRLQHLPQFHHLQTLKTLCLRDLRSLKFIDK 1094

Query: 841  --NEPSSSSSFPSLELLIIANMPNLEGWWEL-----GETQKN--WLPPTFPKLISLHISG 900
              N  SSS  FPSL+ L +ANM NLEGWWEL     GET +N  WLPPTFP+L  L I  
Sbjct: 1095 FDNPFSSSMFFPSLKSLCLANMANLEGWWELSRAVAGETSENIQWLPPTFPELEILDIYQ 1154

Query: 901  CPKLSLMPK-PASTGAVVFLCDVGVQLV-SALGP-LWGLKSLTLEEIEDLKYLEIMQNLG 960
            CPKL+ MPK P+ST A V LCDVGVQLV S +GP L  L++L LEEI++L  L   QN+ 
Sbjct: 1155 CPKLNSMPKLPSSTKAYVSLCDVGVQLVISTIGPVLLCLRTLRLEEIKNLNCLPFQQNIN 1214

Query: 961  ---------SLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKL 1020
                     + PI L++L++ KC +L++LPEWI I TSLE L I +CPKLKSLP+GM++L
Sbjct: 1215 HSLVISSTTTSPIPLKYLQMDKCLDLVTLPEWIYIFTSLETLSISKCPKLKSLPKGMQRL 1274

Query: 1021 ESLRSFCIGGCPEVEERCKQGGEDWPKIAHIPDFEHF 1030
            ESL+  CI  CP +EERCK+GGEDWP I+H+P+   F
Sbjct: 1275 ESLKRLCIEDCPRLEERCKEGGEDWPNISHVPNIVFF 1306

BLAST of Lsi02G025580 vs. ExPASy TrEMBL
Match: A0A5A7UWP6 (Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00750 PE=4 SV=1)

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 614/1058 (58.03%), Postives = 765/1058 (72.31%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
            MAE IL  +AG IL KL S   +  GML GLKDDL++L  TVS I+  L+DA+   T   
Sbjct: 1    MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNWPTGLD 60

Query: 61   LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
             +  WL+KL   LHDAED++DE   E  HREVMTR+K+AK+VRIFFS SNQLA NY+MAR
Sbjct: 61   SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 120

Query: 121  QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFD---QIMTGRESWSFSNEEEVIGRDD 180
            ++K+I ERLD ID EK     H   + + +        ++ + RE+ S  N+E VIGRDD
Sbjct: 121  RVKRIRERLDVIDKEKSYLWSHSFVQGRQQDVPSPIRMRMSSSRETHSSLNKERVIGRDD 180

Query: 181  DKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSD 240
            DK+ +K LLL  +M++  NVS +AIVGMGGIGKTTLAKSLYND  +S++FE+K+W+WVS 
Sbjct: 181  DKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSEHFEIKMWIWVSK 240

Query: 241  QFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLK 300
            QF  K VV+KI+ESATK  P  + ++ +Q++LQ+VIGGKKYLLVMD++ NESE++W  LK
Sbjct: 241  QFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKKLK 300

Query: 301  PLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEG-KEVEN 360
             LL+ G +GSKI+ITKRD KVA+EIE MT+  TL+GL +  SWSLF +VAF+E   +  N
Sbjct: 301  DLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDSSWSLFREVAFEENLPKPIN 360

Query: 361  PNLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKN-NELLEITQQDNDMTSVL 420
            P L  +GKEI    GGVPL IRH+GR L SK  EEEWMS  +  + LE+ QQ+ND+ S+L
Sbjct: 361  PKLELMGKEISSICGGVPLAIRHMGRFLCSKRCEEEWMSSLSWKKHLEVAQQNNDVASIL 420

Query: 421  KLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFK 480
            +LSYN LP +LKQCFAY SLFPKG  LK+N+LIRQW+A GFIESS G K ME  G +YF 
Sbjct: 421  ELSYNDLPLHLKQCFAYSSLFPKGHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYFD 480

Query: 481  ELCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKK-YVCENLNRDYAFNEKTRHVSF 540
            ELCWRFF ENS +E NFD+ V++HDVM +LA  VA KK +VC ++N +Y  +E+T H S 
Sbjct: 481  ELCWRFFYENSSDENNFDDYVYMHDVMYELARKVADKKLFVCGDINNNYVVSEQTLHFSL 540

Query: 541  DCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNN 600
            D KI+SW DVLSKL  AKGLRTF    S   YE+KNE+NEA LDE+FSSFPRLRVL L  
Sbjct: 541  DYKIQSWQDVLSKLCKAKGLRTFMFLHS--PYEEKNEVNEANLDELFSSFPRLRVLLLGG 600

Query: 601  SNSRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNL 660
            SN   +PNSI++L+ LRYLDLSENNM+SLPNSITELQ+LQTL L  C  LKE+PRD   L
Sbjct: 601  SNIHVLPNSIKKLKLLRYLDLSENNMKSLPNSITELQDLQTLKLTGCYALKEMPRDINKL 660

Query: 661  VNLRHLDFN-TSTLTHM-PEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRII 720
            VNLRHLD    S +THM PEG+ +LSCLQT+S FV DC ++++L ELN L +L G+L+I 
Sbjct: 661  VNLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFVFDCTKTNKLRELNELNNLTGELKIT 720

Query: 721  GLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVES 780
            GL+ LR  P EVSLVNLK  K L++L+LEWK+     ++E E DE  +M+GLEP+PNVES
Sbjct: 721  GLQQLRSTPYEVSLVNLKGKKCLRSLKLEWKMGL--YKHEDEADE-RVMKGLEPNPNVES 780

Query: 781  LRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFID 840
            L I GY+GVGL  W+ +   KLT+I +  CHRLQHL Q  HLQ L  L L D+RSLKFID
Sbjct: 781  LGIKGYNGVGLPKWMFNCHLKLTEIEMVSCHRLQHLPQFHHLQTLKTLCLRDLRSLKFID 840

Query: 841  K--NEPSSSSSFPSLELLIIANMPNLEGWWEL-----GETQKN--WLPPTFPKLISLHIS 900
            K  N  SSS  FPSL+ L +ANM NLEGWWEL     GET +N  WLPPTFP+L  L I 
Sbjct: 841  KFDNPFSSSMFFPSLKSLCLANMANLEGWWELSRAVAGETSENIQWLPPTFPELEILDIY 900

Query: 901  GCPKLSLMPK-PASTGAVVFLCDVGVQLV-SALGP-LWGLKSLTLEEIEDLKYLEIMQNL 960
             CPKL+ MPK P+ST A V LCDVGVQLV S +GP L  L++L LEEI+DL  L   QN+
Sbjct: 901  QCPKLNSMPKLPSSTKAYVSLCDVGVQLVISTIGPVLLCLRTLRLEEIKDLNCLPFQQNI 960

Query: 961  G---------SLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEK 1020
                      + PI L++L+I KC +L++LPEWI I TSLE L I +CPKLKSLP+GM +
Sbjct: 961  NHSLVISSTTTSPIPLKYLQIDKCHDLVTLPEWIYIFTSLETLSISKCPKLKSLPKGMRR 1020

Query: 1021 LESLRSFCIGGCPEVEERCKQGGEDWPKIAHIPDFEHF 1030
            LESL+  CI  CP +EERCK+GGEDWP I+H+P+   F
Sbjct: 1021 LESLKRLCIEDCPRLEERCKEGGEDWPNISHVPNIVFF 1053

BLAST of Lsi02G025580 vs. ExPASy TrEMBL
Match: A0A6J1JVS7 (putative disease resistance protein RGA3 OS=Cucurbita maxima OX=3661 GN=LOC111488319 PE=4 SV=1)

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 623/1105 (56.38%), Postives = 769/1105 (69.59%), Query Frame = 0

Query: 7    FDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSHLLENWL 66
            +D+    L KLSS A Q+LGML+G+KD+L +L  +VS IK V+LDAE  QTKSH L++WL
Sbjct: 226  YDMLRISLKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAE-EQTKSHFLKDWL 285

Query: 67   QKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMARQIKKIW 126
            +KL+EAL+DAEDVLDE+S EA+      R + AKQVRIFFS+ N +A +Y+M  QIK + 
Sbjct: 286  EKLKEALYDAEDVLDEISCEAVR-----RGRKAKQVRIFFSRYNPIAFDYKMTCQIKNVR 345

Query: 127  ERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKKKVKDLL 186
            ERL+ I AE+K FHL E    KT++GSFDQI  G E+ SFSN EEVIGRDD K+ +KDLL
Sbjct: 346  ERLEVIAAERKNFHLTEIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKRVIKDLL 405

Query: 187  LDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFEIKAVVE 246
             D  MNV  N+SF+AIVGM GIGKTTLA+SL+ND ++S+ F+LKIWVWVS  FE+K VVE
Sbjct: 406  QD--MNVKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFEVKIVVE 465

Query: 247  KIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLLMSGAKG 306
            KIIESAT S P+V GME+LQT+LQ+++ GKKYLLVMDD+WNESE+KW  LK LLM GA+G
Sbjct: 466  KIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLLMGGARG 525

Query: 307  SKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLIHLGKEI 366
            SKI+ITKRDS +A EI +MTSL TL+GL +  SWSLF KVAF +G E ENP LI LGKEI
Sbjct: 526  SKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLIQLGKEI 585

Query: 367  LVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYNHLPPNL 426
            LVK GGVPLVIRHIGRLLY + SEEEW+SFKNN+LLE+ Q+D+DM S+LKLSYNHL  NL
Sbjct: 586  LVKCGGVPLVIRHIGRLLYIQKSEEEWISFKNNQLLEVIQRDHDMASILKLSYNHLSSNL 645

Query: 427  KQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWRFFLENS 486
            KQCFAY SL  K + +  ++LIRQW+A GF++S +G K MEDT NDYF ELCWRFF EN 
Sbjct: 646  KQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLSGCKSMEDTANDYFMELCWRFFYENF 705

Query: 487  INECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKSWSDVLS 546
             +E NF++DV++HDVM DLA  VAG +YV  N N D+   E TRH+ FD +I  W  V S
Sbjct: 706  NDEHNFEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVAESTRHILFDREIGIWEQVPS 765

Query: 547  KLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTVPNSIRR 606
            KL  AKGLRTF L  SN   ++KN++NE +LDE+F  F RLRVL L  SN + VP+SI+R
Sbjct: 766  KLLKAKGLRTFIL--SNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRVPDSIKR 825

Query: 607  LRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRHLDFNTST 666
            L HLRYLDLS N M+SLPNS+T L NLQTLNL  C  LK+LPR TRNLVNLRHL+ + ST
Sbjct: 826  LTHLRYLDLSGNYMKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRHLNISDST 885

Query: 667  -LTHMPEGIGRLSCLQTLSYFVLDC---KRSSQLSELNVLIHLNGDLRIIGLEYLRHNPS 726
             LTHMPEG+G+L+CLQTLS FVLD    +  S+L ELN L  L G L IIGLE L+  PS
Sbjct: 886  HLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLENLKFTPS 945

Query: 727  EVSLVNLKDIKGLKNLQLEWK-LSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDGYSGV 786
            E SL NLK+ KGL++L+L WK LS D+  ++ +++   I+ GLEP+P +ES RI GY GV
Sbjct: 946  EASLANLKEKKGLRHLKLIWKNLSGDEHRFQADEE---ILAGLEPNPAIESFRISGYFGV 1005

Query: 787  GLTNW--VSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDKNEP-SS 846
             L NW  +SS L KLT+I+I  C RLQ+L Q+ HL AL  LYL ++ SLKFID  +P SS
Sbjct: 1006 KLPNWLSLSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKFIDNTDPYSS 1065

Query: 847  SSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMP-KPAST 906
            +  FPSLE L + +M NLE WWE     +    PTFP L  L IS CPKL+ MP  P  T
Sbjct: 1066 ALFFPSLESLCLMDMLNLEAWWERESMVEEESLPTFPCLTILSISNCPKLATMPGHPPLT 1125

Query: 907  GAVVFLCDVGVQLVSA------LGPLWGLKSLTLEEIEDLKYL--EIMQNLG-------- 966
               + L  VG+Q++S+        P   LKSL L+ IEDLK L  ++ QNL         
Sbjct: 1126 D--ITLDGVGLQMMSSRRSSSNTSPSSSLKSLELKGIEDLKNLSHKLRQNLNSLKFLYIT 1185

Query: 967  --------------------------------------------------SLPIQLRH-- 1026
                                                              SLPI+++H  
Sbjct: 1186 ECENLVNSLFDDTRNSSTSTRNYTELGDAMQWKFLLNLKFLRLENLPKLVSLPIEVQHWT 1245

Query: 1027 ----LKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSFCIGGCPE 1031
                L I KC N  SLPEWIG + SL++LEI+ECPKLKSLPEG+ +L++L    I GC E
Sbjct: 1246 ALKFLIISKCPNFSSLPEWIGGMVSLQELEILECPKLKSLPEGIRELKALNHLRIAGCRE 1305

BLAST of Lsi02G025580 vs. NCBI nr
Match: XP_038897566.1 (putative disease resistance protein RGA4 [Benincasa hispida])

HSP 1 Score: 1854.7 bits (4803), Expect = 0.0e+00
Identity = 932/1037 (89.87%), Postives = 977/1037 (94.21%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
            MAEAILFD+AG ILMKLSSQAFQRLGMLFGLKD+LNKLT TVSTIKDVLLDAEGRQTK H
Sbjct: 1    MAEAILFDIAGEILMKLSSQAFQRLGMLFGLKDNLNKLTATVSTIKDVLLDAEGRQTKGH 60

Query: 61   LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
            LLENWLQKLEEAL+DAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQ+A NY MAR
Sbjct: 61   LLENWLQKLEEALYDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQIAFNYSMAR 120

Query: 121  QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
            QIKKIWERLDAIDAEKKQFHLHENCESK RYGSFDQIMTGRESWSFSN EEVIGRDDDKK
Sbjct: 121  QIKKIWERLDAIDAEKKQFHLHENCESKARYGSFDQIMTGRESWSFSNGEEVIGRDDDKK 180

Query: 181  KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
            K+KDLLLDVNMNVTHNVS VAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIW+WVSDQFE
Sbjct: 181  KIKDLLLDVNMNVTHNVSIVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWIWVSDQFE 240

Query: 241  IKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
            IKAVV+KIIESATKSNPHV GMEALQ ELQ+VIGGKKYLLVMDDVWNESEEKWHGLKPLL
Sbjct: 241  IKAVVKKIIESATKSNPHVMGMEALQAELQKVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300

Query: 301  MSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLI 360
            MSGAKGSKILITKRD KVAAEIESMTSLF+LEGLP+ KSWSLFSKVAFKEGKE+ENPNLI
Sbjct: 301  MSGAKGSKILITKRDRKVAAEIESMTSLFSLEGLPESKSWSLFSKVAFKEGKELENPNLI 360

Query: 361  HLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYN 420
              GKEIL+K GGVPLVIRHIGRLLYS+T+EEEWMSFK+NELLEI QQDNDMTSVLKLSYN
Sbjct: 361  QTGKEILMKCGGVPLVIRHIGRLLYSRTTEEEWMSFKDNELLEIIQQDNDMTSVLKLSYN 420

Query: 421  HLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWR 480
            HLPPNLKQCFAYLSLFPK KSLKINDLIRQWMALGFIE+SNG+K ME+TG  YFKELCWR
Sbjct: 421  HLPPNLKQCFAYLSLFPKRKSLKINDLIRQWMALGFIETSNGSKSMEETGKGYFKELCWR 480

Query: 481  FFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKS 540
            FFLENS+NECNFDNDVH+HDVMRDLATNVAGKKYV EN+N DYAF+EKTRHVSFDCKIKS
Sbjct: 481  FFLENSMNECNFDNDVHMHDVMRDLATNVAGKKYVRENINCDYAFSEKTRHVSFDCKIKS 540

Query: 541  WSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTV 600
            WSDVLSKL  AKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNS  V
Sbjct: 541  WSDVLSKLHKAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSPMV 600

Query: 601  PNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRHL 660
            PNSIRRLRHLRYLDLSENNMESLPNSITEL+NLQTLNLMYC +LKELPRDT NLVNLRHL
Sbjct: 601  PNSIRRLRHLRYLDLSENNMESLPNSITELRNLQTLNLMYCGDLKELPRDTSNLVNLRHL 660

Query: 661  DFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYLRHN 720
            DFNTS LTHMPEG+G+LSCLQTLSYFVLDCKRS+QLSEL+VLIHLNG LRIIGLE LR +
Sbjct: 661  DFNTSILTHMPEGMGKLSCLQTLSYFVLDCKRSNQLSELDVLIHLNGYLRIIGLEQLR-S 720

Query: 721  PSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDGYSG 780
             SEVSLVNLKDIKGLK L+LEWKLS DDQEYEGE+DETAIMEGLEPHPN+ESL IDGYSG
Sbjct: 721  ESEVSLVNLKDIKGLKKLELEWKLSGDDQEYEGENDETAIMEGLEPHPNIESLHIDGYSG 780

Query: 781  VGLTNW-VSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDKNEPSSS 840
            VGL NW VS+SLSKLT I+I+KCHRLQHL+QMGHLQALTFLYLDDMRSLKFIDKNEPS S
Sbjct: 781  VGLPNWVVSTSLSKLTNIVIHKCHRLQHLSQMGHLQALTFLYLDDMRSLKFIDKNEPSFS 840

Query: 841  SSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMPKPASTGA 900
            SSFPSL+LLII NMPNLEGWWELG TQKNWLP TFPKLISLHISGCP  S MPKPASTGA
Sbjct: 841  SSFPSLKLLIIENMPNLEGWWELGNTQKNWLPLTFPKLISLHISGCPMFSFMPKPASTGA 900

Query: 901  VVFLCDVGVQLVSALGPLWGLKSLTLEEIEDLKYLEIM---QNLGSLPIQLRHLKIRKCS 960
            VVFLCD  VQL++ LGPL  L+SLTLEEI+DLKYLEIM   QNL SLPI+LRHL+IRKC 
Sbjct: 901  VVFLCDASVQLITILGPLCSLESLTLEEIKDLKYLEIMESQQNLDSLPIRLRHLRIRKCL 960

Query: 961  NLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSFCIGGCPEVEERCKQGGED 1020
            NLMSLPEWIGILTSLE+LEIMECPKLKSLPEGM+KLESLRSF IGGCPEVEERCKQGGED
Sbjct: 961  NLMSLPEWIGILTSLEELEIMECPKLKSLPEGMQKLESLRSFLIGGCPEVEERCKQGGED 1020

Query: 1021 WPKIAHIPDFEHFLLDN 1034
            W KIAHIP FE FLLDN
Sbjct: 1021 WAKIAHIPHFEQFLLDN 1036

BLAST of Lsi02G025580 vs. NCBI nr
Match: XP_031745107.1 (putative disease resistance protein RGA4 [Cucumis sativus])

HSP 1 Score: 1401.0 bits (3625), Expect = 0.0e+00
Identity = 729/1050 (69.43%), Postives = 846/1050 (80.57%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
            MAEAILF VAG ILMKLSSQAFQRLGMLFGLK DLNKLT TVSTIKDVLLDAEGRQTKSH
Sbjct: 1    MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60

Query: 61   LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRD-KNAKQVRIFFSKSNQLALNYRMA 120
            LL+NWL KLEEAL+DAEDVLDELSTEAL RE+MTRD KNAKQVRIFFSKSNQ+A NYRMA
Sbjct: 61   LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMA 120

Query: 121  RQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDK 180
            RQIK IWERLDAIDAEK QFHL ENCES+T+YGSFD+IM GRE+WS SN+EEVIGRDDD 
Sbjct: 121  RQIKNIWERLDAIDAEKTQFHLRENCESRTQYGSFDRIMMGRETWSSSNDEEVIGRDDDI 180

Query: 181  KKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQF 240
            K+VK+ LLD+NMNVTHNVSF+AI GMGGIGKTTLAKSLYNDE++S +F+LKIWVWVSDQF
Sbjct: 181  KEVKERLLDMNMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQF 240

Query: 241  EIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPL 300
            E++ V EK+IESATK+NP VKGMEALQ +LQ+VIG +KYLLVMDDVWNESEEKWHGLK L
Sbjct: 241  EVQVVAEKMIESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKSL 300

Query: 301  LMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNL 360
            LM GA+GSK+LITKRD KVA EI+SMTSLFTLEGL +  SW LFSKVAFKEGKE  +P+ 
Sbjct: 301  LMGGARGSKVLITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPST 360

Query: 361  IHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSY 420
            IHLGKEILV+ GGVPLVIRH+GR+LYSKTS+EEWMSFK+NELLE+ QQDNDMTS+LKLSY
Sbjct: 361  IHLGKEILVRCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEVIQQDNDMTSILKLSY 420

Query: 421  NHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCW 480
            NHLPPNLK+CFAY SLFPKG  ++I DLIRQW+A GFIE SNG K +EDTG DYF ELCW
Sbjct: 421  NHLPPNLKRCFAYSSLFPKGYKIEIKDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCW 480

Query: 481  RFFLENSINECNFDNDVHVHDVMRDLATNVAGKK-YVCENLNRDYAFNEKTRHVSFDCKI 540
            RFF  NS +ECN ++ V +HDVM +    VAG K YV  N N DY  +E+T H+SFD  I
Sbjct: 481  RFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGI 540

Query: 541  KSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSR 600
            +SW DVLSKL  AKGLRT  L      YEK N+I++AILDE+FSSFPRLRVL L+ S   
Sbjct: 541  QSWQDVLSKLCKAKGLRTILLLFR--PYEKMNKIDKAILDELFSSFPRLRVLDLHFSQIS 600

Query: 601  TVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLR 660
             VP SI++LRHLRYLDLSEN+ME +P+SI ELQNLQTLNL  C ELKELPRD  NLVNLR
Sbjct: 601  VVPKSIKKLRHLRYLDLSENDMELIPHSIIELQNLQTLNLTECYELKELPRDIDNLVNLR 660

Query: 661  HLDFN-TSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYL 720
            HL F     +T   EG+ +L+CLQT+S FV DCK++++L ELN L +L G+L+IIGLE L
Sbjct: 661  HLTFEPCMEVTPTSEGMEKLTCLQTISLFVFDCKKTNKLWELNDLSYLTGELKIIGLEKL 720

Query: 721  RHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDG 780
            R +PSE++L+NLKD KG + L LEWKL +D  EYEGE DET IMEGLEPHPNVESL I+G
Sbjct: 721  RSSPSEITLINLKDKKGWQGLNLEWKLGKD--EYEGEADET-IMEGLEPHPNVESLSING 780

Query: 781  YSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDKNEP- 840
            Y+G  L NWV +SL KLT+I I  C R+QHL Q   LQ L  L+L  +RSL+FIDK++P 
Sbjct: 781  YTGGALPNWVFNSLMKLTEIEIENCPRVQHLPQFNQLQDLRALHLVGLRSLEFIDKSDPY 840

Query: 841  SSSSSFPSLELLIIANMPNLEGWWELGET---------QKNWLPPTFPKLISLHISGCPK 900
            SSS  FPSL+ L + +MPNLEGWWELGE+         +  WLPPTFP++  L I GCPK
Sbjct: 841  SSSVFFPSLKFLRLEDMPNLEGWWELGESKVVARETSGKAKWLPPTFPQVNFLRIYGCPK 900

Query: 901  LSLMPKPASTGAVVFLCDVGVQLVSALGPLWGLKSLTLEEIEDLKYL--EIMQNL----- 960
            LS MPK AS GA V L D+GVQ+VS +GP+     L++  + +LKYL  E  Q+L     
Sbjct: 901  LSSMPKLASIGADVILHDIGVQMVSTIGPVSSFMFLSMHGMTNLKYLWEEFQQDLVSSST 960

Query: 961  --GSLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSF 1020
               S PI LR+L I  C  LMSLPEWIG+LTSLE L I ECPKLKSLPEGM++L+SL+  
Sbjct: 961  STMSSPISLRYLTISGCPYLMSLPEWIGVLTSLETLHIKECPKLKSLPEGMQQLKSLKEL 1020

Query: 1021 CIGGCPEVEERCKQGGEDWPKIAHIPDFEH 1029
             I  CPE+E+RCKQGGEDWP I+H+P+F +
Sbjct: 1021 HIEDCPELEDRCKQGGEDWPNISHVPNFTY 1045

BLAST of Lsi02G025580 vs. NCBI nr
Match: KAE8646395.1 (hypothetical protein Csa_016171 [Cucumis sativus])

HSP 1 Score: 1401.0 bits (3625), Expect = 0.0e+00
Identity = 729/1050 (69.43%), Postives = 846/1050 (80.57%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
            MAEAILF VAG ILMKLSSQAFQRLGMLFGLK DLNKLT TVSTIKDVLLDAEGRQTKSH
Sbjct: 1    MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60

Query: 61   LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRD-KNAKQVRIFFSKSNQLALNYRMA 120
            LL+NWL KLEEAL+DAEDVLDELSTEAL RE+MTRD KNAKQVRIFFSKSNQ+A NYRMA
Sbjct: 61   LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMA 120

Query: 121  RQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDK 180
            RQIK IWERLDAIDAEK QFHL ENCES+T+YGSFD+IM GRE+WS SN+EEVIGRDDD 
Sbjct: 121  RQIKNIWERLDAIDAEKTQFHLRENCESRTQYGSFDRIMMGRETWSSSNDEEVIGRDDDI 180

Query: 181  KKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQF 240
            K+VK+ LLD+NMNVTHNVSF+AI GMGGIGKTTLAKSLYNDE++S +F+LKIWVWVSDQF
Sbjct: 181  KEVKERLLDMNMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQF 240

Query: 241  EIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPL 300
            E++ V EK+IESATK+NP VKGMEALQ +LQ+VIG +KYLLVMDDVWNESEEKWHGLK L
Sbjct: 241  EVQVVAEKMIESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKSL 300

Query: 301  LMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNL 360
            LM GA+GSK+LITKRD KVA EI+SMTSLFTLEGL +  SW LFSKVAFKEGKE  +P+ 
Sbjct: 301  LMGGARGSKVLITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPST 360

Query: 361  IHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSY 420
            IHLGKEILV+ GGVPLVIRH+GR+LYSKTS+EEWMSFK+NELLE+ QQDNDMTS+LKLSY
Sbjct: 361  IHLGKEILVRCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEVIQQDNDMTSILKLSY 420

Query: 421  NHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCW 480
            NHLPPNLK+CFAY SLFPKG  ++I DLIRQW+A GFIE SNG K +EDTG DYF ELCW
Sbjct: 421  NHLPPNLKRCFAYSSLFPKGYKIEIKDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCW 480

Query: 481  RFFLENSINECNFDNDVHVHDVMRDLATNVAGKK-YVCENLNRDYAFNEKTRHVSFDCKI 540
            RFF  NS +ECN ++ V +HDVM +    VAG K YV  N N DY  +E+T H+SFD  I
Sbjct: 481  RFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGI 540

Query: 541  KSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSR 600
            +SW DVLSKL  AKGLRT  L      YEK N+I++AILDE+FSSFPRLRVL L+ S   
Sbjct: 541  QSWQDVLSKLCKAKGLRTILLLFR--PYEKMNKIDKAILDELFSSFPRLRVLDLHFSQIS 600

Query: 601  TVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLR 660
             VP SI++LRHLRYLDLSEN+ME +P+SI ELQNLQTLNL  C ELKELPRD  NLVNLR
Sbjct: 601  VVPKSIKKLRHLRYLDLSENDMELIPHSIIELQNLQTLNLTECYELKELPRDIDNLVNLR 660

Query: 661  HLDFN-TSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYL 720
            HL F     +T   EG+ +L+CLQT+S FV DCK++++L ELN L +L G+L+IIGLE L
Sbjct: 661  HLTFEPCMEVTPTSEGMEKLTCLQTISLFVFDCKKTNKLWELNDLSYLTGELKIIGLEKL 720

Query: 721  RHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDG 780
            R +PSE++L+NLKD KG + L LEWKL +D  EYEGE DET IMEGLEPHPNVESL I+G
Sbjct: 721  RSSPSEITLINLKDKKGWQGLNLEWKLGKD--EYEGEADET-IMEGLEPHPNVESLSING 780

Query: 781  YSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDKNEP- 840
            Y+G  L NWV +SL KLT+I I  C R+QHL Q   LQ L  L+L  +RSL+FIDK++P 
Sbjct: 781  YTGGALPNWVFNSLMKLTEIEIENCPRVQHLPQFNQLQDLRALHLVGLRSLEFIDKSDPY 840

Query: 841  SSSSSFPSLELLIIANMPNLEGWWELGET---------QKNWLPPTFPKLISLHISGCPK 900
            SSS  FPSL+ L + +MPNLEGWWELGE+         +  WLPPTFP++  L I GCPK
Sbjct: 841  SSSVFFPSLKFLRLEDMPNLEGWWELGESKVVARETSGKAKWLPPTFPQVNFLRIYGCPK 900

Query: 901  LSLMPKPASTGAVVFLCDVGVQLVSALGPLWGLKSLTLEEIEDLKYL--EIMQNL----- 960
            LS MPK AS GA V L D+GVQ+VS +GP+     L++  + +LKYL  E  Q+L     
Sbjct: 901  LSSMPKLASIGADVILHDIGVQMVSTIGPVSSFMFLSMHGMTNLKYLWEEFQQDLVSSST 960

Query: 961  --GSLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSF 1020
               S PI LR+L I  C  LMSLPEWIG+LTSLE L I ECPKLKSLPEGM++L+SL+  
Sbjct: 961  STMSSPISLRYLTISGCPYLMSLPEWIGVLTSLETLHIKECPKLKSLPEGMQQLKSLKEL 1020

Query: 1021 CIGGCPEVEERCKQGGEDWPKIAHIPDFEH 1029
             I  CPE+E+RCKQGGEDWP I+H+P+F +
Sbjct: 1021 HIEDCPELEDRCKQGGEDWPNISHVPNFTY 1045

BLAST of Lsi02G025580 vs. NCBI nr
Match: XP_022147051.1 (putative disease resistance protein RGA4 [Momordica charantia])

HSP 1 Score: 1351.3 bits (3496), Expect = 0.0e+00
Identity = 720/1053 (68.38%), Postives = 836/1053 (79.39%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGR-QTKS 60
            MAE +LF VAG ILMKL S A QRLGM+ G+++DL KLT TVS IK+VLLDAE   QTK+
Sbjct: 1    MAEGVLFHVAGEILMKLGSIASQRLGMVRGVENDLTKLTSTVSVIKNVLLDAEEHCQTKT 60

Query: 61   -HLLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRM 120
             H L++WLQKLEEAL+DAEDVLDELS+E LHREV+T+ KNAKQVRIFFS SNQ+A NYR+
Sbjct: 61   DHTLQDWLQKLEEALYDAEDVLDELSSETLHREVVTKGKNAKQVRIFFSGSNQIAFNYRV 120

Query: 121  ARQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGR-ESWSFSNEEEVIGRDD 180
            AR IKKIWERLDAIDAE+KQF+L ENC+ +T YGSFDQIM GR E+ SF N EEVIGRD 
Sbjct: 121  ARNIKKIWERLDAIDAERKQFNLTENCQQRTLYGSFDQIMMGRKETCSFPNVEEVIGRDY 180

Query: 181  DKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSD 240
            DKKK+KDLLLD+NMNV HNVSF+ I GMGGIGKTTLAKSLY+D ++S+ F+  IW+W+SD
Sbjct: 181  DKKKLKDLLLDINMNVRHNVSFIVIAGMGGIGKTTLAKSLYHDREVSECFDSTIWIWISD 240

Query: 241  QFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLK 300
            QFE++ VVEKIIESATK  P+V+GMEAL +ELQEVIGGKKYLLVMDDVW E+EEKWH LK
Sbjct: 241  QFEVRVVVEKIIESATKRKPNVEGMEALNSELQEVIGGKKYLLVMDDVWKETEEKWHELK 300

Query: 301  PLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENP 360
            PLLM GA GSKILITKRDSKVA EIESMTSLFTL+GL +  SWSLF KVAFKE K+  N 
Sbjct: 301  PLLMGGASGSKILITKRDSKVATEIESMTSLFTLKGLQESNSWSLFRKVAFKEAKDPANS 360

Query: 361  NLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQ---DNDMTSV 420
            NLI LGKEIL K GGVPLVIRHIGRLLYSKTSEEEWMSFKNNELL++ QQ   ++ +T +
Sbjct: 361  NLIKLGKEILAKCGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLKVIQQTDKNDGLTPI 420

Query: 421  LKLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGT-KCMEDTGNDY 480
            L LSYNHLPPNLKQCFAYLSLFPKG  L+I DLIRQWMA GFIESSNGT   ME+ G  Y
Sbjct: 421  LNLSYNHLPPNLKQCFAYLSLFPKGTRLEIKDLIRQWMAHGFIESSNGTANSMEEVGKSY 480

Query: 481  FKELCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVS 540
            FKELCWRFF ENS +ECNFD+DV +HDVMR++A NVAG+KYV  NLN D+  +EKTRH+ 
Sbjct: 481  FKELCWRFFFENSSDECNFDDDVDMHDVMREVAMNVAGEKYVHGNLNCDFVVSEKTRHIL 540

Query: 541  FDCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEIN-EAILDEVFSSFPRLRVLGL 600
            FD KIK W DVL KL+ AKGLRTF LF S F ++ + E N EA+L+E+ SSFPRLRVLGL
Sbjct: 541  FDSKIKLWQDVLLKLQKAKGLRTF-LFLSYFPFKNRLEDNIEAVLEELCSSFPRLRVLGL 600

Query: 601  NNSNSRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTR 660
            N+S    VPNSI+ LRHLRYL+LS N MESLP SIT+LQNLQ+L+L  C +L ELPRDT 
Sbjct: 601  NHSKIHVVPNSIKNLRHLRYLNLSRNYMESLPTSITKLQNLQSLDLKDCYKLNELPRDTS 660

Query: 661  NLVNLRHLDF--NTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLR 720
            NLVNLRHL    +  T  HM EG+ +LSCL+TL YFVLDCKRS+  SELNV  + NG L+
Sbjct: 661  NLVNLRHLVLPDHLDTPLHMLEGMRKLSCLRTLGYFVLDCKRSNMQSELNVPNYFNGRLK 720

Query: 721  IIGLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLS-RDDQEYEGEDDETAIMEGLEPHPN 780
            I+GLE LR  PS+V LVNLKDI+GL++L+L WKLS RDD E EG+ DE  +MEGL+P  N
Sbjct: 721  IVGLEQLRRTPSKVGLVNLKDIQGLRDLKLVWKLSGRDDDEGEGKGDE-RVMEGLQPQSN 780

Query: 781  VESLRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLK 840
            VESLRI+GYSGVGL NWVS+SLS LTKI I  CHR+QHL Q+ HLQ L  ++L+DMRSLK
Sbjct: 781  VESLRIEGYSGVGLPNWVSTSLSNLTKIAICNCHRVQHLPQLAHLQTLKGIFLEDMRSLK 840

Query: 841  FIDKNEPSSSSSFPSLELLIIANMPNLEGWWE---LGETQKNWLPPTFPKLISLHISGCP 900
            F+DKNEP     FPSL  L + NM NLEGWWE   +GET      P FP+L  L I  CP
Sbjct: 841  FVDKNEP-GLLCFPSLVSLRLKNMGNLEGWWESRVVGETL-----PIFPELQYLRIQHCP 900

Query: 901  KLSLMPKPA-STGAVVFLCDVGVQLVSALGPLWGLKSLTLEEIEDLKYLEI------MQN 960
            KLSLMPK A ST    FLCDVGVQ+V+A+GPLW  + + L+EI+DLK LE+       Q+
Sbjct: 901  KLSLMPKIASSTLDSFFLCDVGVQVVTAIGPLWNFEVVILQEIKDLKDLEMESHQNRNQD 960

Query: 961  LGS--------LPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEK 1020
            LGS         P +LR L I KCSNLMSLPEWIG +TSL  + I++CPKLKSL EGM +
Sbjct: 961  LGSSSLSMSSRSPTRLRFLTIEKCSNLMSLPEWIGTITSLWKVTIIDCPKLKSLLEGMRQ 1020

Query: 1021 LESLRSFCIGGCPEVEERCKQGGEDWPKIAHIP 1025
            L+SL+   I GCPE+EERCK+GGEDWP IAHIP
Sbjct: 1021 LKSLKYLTIKGCPELEERCKEGGEDWPNIAHIP 1045

BLAST of Lsi02G025580 vs. NCBI nr
Match: XP_022953917.1 (putative disease resistance protein RGA4 [Cucurbita moschata])

HSP 1 Score: 1151.0 bits (2976), Expect = 0.0e+00
Identity = 636/1111 (57.25%), Postives = 782/1111 (70.39%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
            MAE +LF++A  I+ KLSS A Q+LGML+G+KD+L +L  +VS IK V+LDAE RQTKSH
Sbjct: 1    MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEERQTKSH 60

Query: 61   LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
             L++WL+KL+EAL+DAEDVLDE+S EA+      R + AKQVRIFFS+ N +A +Y+M R
Sbjct: 61   FLKDWLEKLKEALYDAEDVLDEISGEAVR-----RGRKAKQVRIFFSRYNPIAFDYKMTR 120

Query: 121  QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
            QIK + ERL  I AE+K FHL E    KT++GSFDQI  G E+ SFSN EEVIGRDD K+
Sbjct: 121  QIKNVRERLGVIAAERKNFHLTEIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKR 180

Query: 181  KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
             +KDLL D  MNV  N+SF+AIVGM GIGKTTLA+SL+ND ++S+ F+LKIWVWVS  FE
Sbjct: 181  VIKDLLQD--MNVKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFE 240

Query: 241  IKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
            +K VVEKIIESAT S P+V GME+LQT+LQ+++ GKKYLLVMDD+WNESE+KW  LK LL
Sbjct: 241  VKIVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLL 300

Query: 301  MSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLI 360
            M GA+GSKI+ITKRDS +A EI +MTSL TL+GL +  SWSLF KVAF +G E ENP LI
Sbjct: 301  MGGARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLI 360

Query: 361  HLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYN 420
             LGKEILVK GGVPLVIRHIGRLLY + SEEEW+SFKNN+LLE+ Q+D+DM S+LKLSYN
Sbjct: 361  QLGKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEVIQRDHDMASILKLSYN 420

Query: 421  HLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWR 480
            HLP NLKQCFAY SL  K + +  ++LIRQW+A GF++S NG K MEDTGNDYF ELCWR
Sbjct: 421  HLPSNLKQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLNGCKSMEDTGNDYFMELCWR 480

Query: 481  FFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKS 540
            FF EN  +E NF++DV++HDVM DLA  VAG +YV  N N D+   E TRH+ FD +I  
Sbjct: 481  FFYENFNDEHNFEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVGESTRHILFDREIGI 540

Query: 541  WSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTV 600
            W  V SKLR AKGLRTF L  SN   ++KN++NE +LDE+F  F RLRVL L  SN + V
Sbjct: 541  WEHVPSKLRKAKGLRTFIL--SNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRV 600

Query: 601  PNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRHL 660
            P+SI+RL HLRYLDLS N M+SLPNS+T L NLQTLNL  C  LK+LPR TRNLVNLRHL
Sbjct: 601  PDSIKRLTHLRYLDLSGNYMKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRHL 660

Query: 661  DFNTST-LTHMPEGIGRLSCLQTLSYFVLDC---KRSSQLSELNVLIHLNGDLRIIGLEY 720
            + + ST LTHMPEG+G+L+CLQTLS FVLD    +  S+L ELN L  L G L IIGLE 
Sbjct: 661  NISDSTHLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLEN 720

Query: 721  LRHNPSEVSLVNLKDIKGLKNLQLEWK-LSRDDQEYEGEDDETAIMEGLEPHPNVESLRI 780
            L+  PSE SL NLK+ KGL++L L WK LS D+  ++ +++   I+ GLEP+P +ES RI
Sbjct: 721  LKFTPSEASLANLKEKKGLRHLTLIWKNLSGDEHRFQADEE---ILAGLEPNPAIESFRI 780

Query: 781  DGYSGVGLTNW--VSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDK 840
             GY GV L NW  +SS L KLT+I+I  C RLQ+L Q+ HL AL  LYL ++ SLKFID 
Sbjct: 781  SGYFGVKLPNWLSLSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKFIDN 840

Query: 841  NEP-SSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMP 900
             +P SS+  FPSLE L + +MPNLE WWE     +    PTFP L  L IS CPKL+ MP
Sbjct: 841  TDPYSSALFFPSLESLCLMDMPNLEAWWERESMVEEEFLPTFPCLTILSISNCPKLATMP 900

Query: 901  -KPASTGAVVFLCDVGVQLVSAL------GPLWGLKSLTLEEIEDLKYL--EIMQNLG-- 960
              P  T   + L  VG+Q++S++       P   LKSL L+ IEDLK L  ++ QNL   
Sbjct: 901  GHPPLTD--ITLDGVGLQMMSSIRSSSNTSPSSSLKSLELKGIEDLKNLSHKLRQNLNSL 960

Query: 961  --------------------------------------------------------SLPI 1020
                                                                    SLPI
Sbjct: 961  KFLYITECESLVNSLFDETRSSSTSTRNYTELGDAMQWKFLHNLKFLRLENLPKLVSLPI 1020

Query: 1021 QLRH------LKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSFC 1031
            +++H      L I KC N  SLPEWIG + SL++LEI+ECPKLKSLPEG+ +L++L    
Sbjct: 1021 EVQHWTALKFLIISKCPNFRSLPEWIGGMVSLQELEILECPKLKSLPEGIRELKALNHLR 1080

BLAST of Lsi02G025580 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 432.6 bits (1111), Expect = 9.1e-121
Identity = 313/1029 (30.42%), Postives = 518/1029 (50.34%), Query Frame = 0

Query: 1    MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDD-LNKLTGTVSTIKDVLLDAEGRQTKS 60
            + E  L     A+   L S+ F+       L ++ L +L+  + TI  VL+DAE +Q  +
Sbjct: 4    IGEMFLAAFLQALFQTLVSEPFRSFFKRRELNENLLERLSTALLTITAVLIDAEEKQITN 63

Query: 61   HLLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNA---KQVRIFFSKSNQLALN- 120
             ++E W+ +L + ++ AED LD+++TEAL   +     ++   +Q+R   S  + L  N 
Sbjct: 64   PVVEKWVNELRDVVYHAEDALDDIATEALRLNIGAESSSSNRLRQLRGRMSLGDFLDGNS 123

Query: 121  YRMARQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGR 180
              +  +++K+  RL+ + +++    L E     T      ++ T     S  +E EV GR
Sbjct: 124  EHLETRLEKVTIRLERLASQRNILGLKE----LTAMIPKQRLPT----TSLVDESEVFGR 183

Query: 181  DDDKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWV 240
            DDDK ++   L+  N    + ++ VAIVG+GG+GKTTL++ LYND+ +  YF  K+W  V
Sbjct: 184  DDDKDEIMRFLIPEN-GKDNGITVVAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHV 243

Query: 241  SDQFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGK--KYLLVMDDVWNESEEKW 300
            S++F++  + +K+ ES T        ++ LQ +L+E + G    +LLV+DD+WNE+   W
Sbjct: 244  SEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLTGTGLPFLLVLDDLWNENFADW 303

Query: 301  HGLKPLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKE 360
              L+   +  A+GS+IL+T R  +VA+ I     +  L+ L DG  WSLF K  F   + 
Sbjct: 304  DLLRQPFIHAAQGSQILVTTRSQRVAS-IMCAVHVHNLQPLSDGDCWSLFMKTVFGNQEP 363

Query: 361  VENPNLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTS 420
              N  +  L + I+ K  G+PL ++ +G +L  +    EW    ++ + ++    +++  
Sbjct: 364  CLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADKSNLLP 423

Query: 421  VLKLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDY 480
            VL++SY +LP +LK+CFAY S+FPKG + + + ++  WMA GF++ +  +K +E+ GN+Y
Sbjct: 424  VLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEY 483

Query: 481  FKELCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVS 540
            F EL  R  L+ +           +HD + +LA   +G+             +E+TR++S
Sbjct: 484  FSELESRSLLQKT------KTRYIMHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLS 543

Query: 541  FDCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLN 600
            +     +       LR  K LRTF L  S     +   +++ + +++  +  RLRVL L+
Sbjct: 544  YLRDNYAEPMEFEALREVKFLRTF-LPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLS 603

Query: 601  NSN-SRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTR 660
            +   +R  P+  + + H R+LDLS   +E LP S+  + NLQTL L YC  LKELP D  
Sbjct: 604  HYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDIS 663

Query: 661  NLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRII 720
            NL+NLR+LD   + L  MP   GRL  LQTL+ F +     S++SEL  L  L+G L+I+
Sbjct: 664  NLINLRYLDLIGTKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIV 723

Query: 721  GLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGE----DDETAIMEGLEPHP 780
             L+ +  + ++ +  NL   K L+ +   W+      E         +E  + E L PH 
Sbjct: 724  ELQRV-VDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHR 783

Query: 781  NVESLRIDGYSGVGLTNWVSS-SLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRS 840
            ++E L I+ Y G    +W+S  S S++  I + +C     L  +G L  L  L++  M  
Sbjct: 784  HIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVG 843

Query: 841  LKFIDKN--------EPSSSSSFPSLELLIIANMPNLEGWWELGETQKNWLP-------- 900
            L+ I +                F SLE L   N+P+ + W ++  T+ +  P        
Sbjct: 844  LQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFIL 903

Query: 901  ---------PTF-PKLISLHISGCPKLSLMP-------KPASTGAVVFLCDVGVQLVSAL 960
                     PTF P LISLHI  C  L   P       +   T ++   CD  V+    L
Sbjct: 904  RCPELTGTLPTFLPSLISLHIYKCGLLDFQPDHHEYSYRNLQTLSIKSSCDTLVKF--PL 963

Query: 961  GPLWGLKSLTLEEIEDLKYLEIMQNLGSLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDL 984
                 L  L +++   L  LE+       P  LR+L+I  C NL  LP+   +  +L+ +
Sbjct: 964  NHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLPKLNALPQNLQ-V 1011

BLAST of Lsi02G025580 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 364.8 bits (935), Expect = 2.3e-100
Identity = 273/897 (30.43%), Postives = 439/897 (48.94%), Query Frame = 0

Query: 35  LNKLTGTVSTIKDVLLDAEGRQTKSHLLENWLQKLEEALHDAEDVLDELSTEALHREVMT 94
           L +L   + T   VL DA+ R      +++WL  +++A   AED+LDEL TEAL R V+ 
Sbjct: 36  LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95

Query: 95  RDKNAKQVRIFFSK--SNQLALNYRMARQIKKIWERLDAIDAEKKQFHLHENCESKTRYG 154
               A  +   F    + + A+  ++  +++K+   L+      +   L E   S+TR  
Sbjct: 96  ---EAGGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEHHVKHIEVIGLKE--YSETREP 155

Query: 155 SFDQIMTGRESWSFSNEEEVIGRDDDKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTT 214
            + Q    R       +  ++GR +DK  + +LLL  +       + +++VGM G+GKTT
Sbjct: 156 QWRQASRSRP--DDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTT 215

Query: 215 LAKSLYNDEKLSKYFELKIWVWVSDQFEIKAVVEKIIESATKSNPHVKGMEALQTELQEV 274
           L + ++ND +++++FE+K+W+     F +  V + +++  T S  + + + +LQ +L++ 
Sbjct: 216 LTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKT 275

Query: 275 IGGKKYLLVMDDVWNESEEKWHGLKPLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLE 334
           + GK++LLV+DD W+ES+ +W   +       +GSKI++T R S++ + +     ++ ++
Sbjct: 276 LSGKRFLLVLDDFWSESDSEWESFQVAFTDAEEGSKIVLTTR-SEIVSTVAKAEKIYQMK 335

Query: 335 GLPDGKSWSLFSKVAFKE-GKEVENPNLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEE 394
            + + + W L S+ AF        N  L  +GK I  +  G+PL  R I   L SK + +
Sbjct: 336 LMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPD 395

Query: 395 EWMSFKNNELLEITQQDNDMTSVLKLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQW 454
           +W +   N     +   N +  VLKLSY+ LPP LK+CFA  S+FPKG      +L+  W
Sbjct: 396 DWYAVSKN----FSSYTNSILPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLW 455

Query: 455 MALGFIESSNGTKCMEDTGNDYFKEL-CWRFFLENSINECNFDNDVHVHDVMRDLATNVA 514
           MA+  +     ++ +ED GNDY  +L    FF    I   +F     +HD+M DLA  V+
Sbjct: 456 MAIDLLYQPRSSRRLEDIGNDYLGDLVAQSFFQRLDITMTSF----VMHDLMNDLAKAVS 515

Query: 515 GKKYVCENLNRDY--AFNEKTRHVSFDCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEK 574
           G    C  L  D        TRH SF       S     +  A+ LRT   F+S    E 
Sbjct: 516 GD--FCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLES 575

Query: 575 KNEINEAILDEVFSSFPRLRVLGLNNSNSRTVPNSIRRLRHLRYLDLSENNMESLPNSIT 634
             ++ E +L+ + ++   LR+L L++     +P S++ L+ LRYLDLS   ++ LP  + 
Sbjct: 576 L-QLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSSTKIKELPEFVC 635

Query: 635 ELQNLQTLNLMYCRELKELPRDTRNLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVL 694
            L NLQTL L  CR+L  LP+    L+NLR LD   + L  MP GI +L  LQ LS FV+
Sbjct: 636 TLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGIKKLRSLQKLSNFVI 695

Query: 695 DCKRSSQLSELNVLIHLNGDLRIIGLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDD 754
                + L EL  L HL G LRI  L+ +    SE     LK    L  L L+W +    
Sbjct: 696 GRLSGAGLHELKELSHLRGTLRISELQNVAF-ASEAKDAGLKRKPFLDGLILKWTVKGSG 755

Query: 755 QEYEGED----DETAIMEGLEPHPNVESLRIDGYSGVGLTNWV-SSSLSKLTKIIIYKCH 814
                 +    D+  ++  LEPHP++++  I+ Y G     W+  SS   +T + +  C+
Sbjct: 756 FVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFFGITSVTLSSCN 815

Query: 815 RLQHLTQMGHLQALTFLYLDDMR-----SLKFIDKNEPSSSSSFPSLELLIIANMPNLEG 874
               L  +G L +L +L ++         L F      S    F SL++L    MP  + 
Sbjct: 816 LCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVPFQSLQILKFYGMPRWDE 875

Query: 875 WW--ELGETQKNWLPPTFPKLISLHISGCPKL-SLMPKPASTGAVVFLCDVGVQLVS 913
           W   EL +         FP L  L I  CP L    P+   +   V + D  ++ VS
Sbjct: 876 WICPELED-------GIFPCLQKLIIQRCPSLRKKFPEGLPSSTEVTISDCPLRAVS 905


HSP 2 Score: 66.6 bits (161), Expect = 1.3e-10
Identity = 67/212 (31.60%), Postives = 98/212 (46.23%), Query Frame = 0

Query: 808  LTQMGHL----QALTFLYLDDMRSLKFIDKNEPSSSSSFPSLELLIIANMPNLEGWWELG 867
            +T + HL    Q L  L++D    L  + +N    + S+P+L  L+I    +LE +    
Sbjct: 1080 VTDISHLMELPQNLQSLHIDSCDGLTSLPEN---LTESYPNLHELLIIACHSLESF---- 1139

Query: 868  ETQKNWLPPTFPKLISLHISGCPKL----SLMP-KPASTGAVVFL---CDVGVQLVSALG 927
                   PPT   L +L+I  C KL    SL P +  S    +F+   C   V    +L 
Sbjct: 1140 ---PGSHPPT--TLKTLYIRDCKKLNFTESLQPTRSYSQLEYLFIGSSCSNLVNFPLSLF 1199

Query: 928  PLWGLKSLTLEEIEDLKYLEIMQNLGSLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLE 987
            P   L+SL++ + E  K   I   LG   I L  L+IR C NL + P+       L  + 
Sbjct: 1200 P--KLRSLSIRDCESFKTFSIHAGLGDDRIALESLEIRDCPNLETFPQGGLPTPKLSSML 1259

Query: 988  IMECPKLKSLPEGMEKLESLRSFCIGGCPEVE 1008
            +  C KL++LPE +  L SL S  I  CPE+E
Sbjct: 1260 LSNCKKLQALPEKLFGLTSLLSLFIIKCPEIE 1277

BLAST of Lsi02G025580 vs. TAIR 10
Match: AT1G58848.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 245.7 bits (626), Expect = 1.6e-64
Identity = 274/1085 (25.25%), Postives = 466/1085 (42.95%), Query Frame = 0

Query: 23   QRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSHLLENWLQKLEEALHDAEDVLDE 82
            Q   +  G++D + +L   ++ +   L DA+ ++  S +++N +++++E ++D ED ++ 
Sbjct: 19   QECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSAVVKNCVEEIKEIIYDGEDTIET 78

Query: 83   LSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMARQIKKIWERLDAIDAEKKQFHLH 142
               E    +     K+ +++        + AL       I  +  R+  +  + + F + 
Sbjct: 79   FVLEQNLGKTSGIKKSIRRLACIIPDRRRYALG------IGGLSNRISKVIRDMQSFGVQ 138

Query: 143  EN-CESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKKKVKDLLLDVNMNVTHNVSFVA 202
            +   +   +    D+    R  +S  ++ + +G + + KK+   L+D       NV  V+
Sbjct: 139  QAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVGYLVD-----EANVQVVS 198

Query: 203  IVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFEIKAVVEKIIESATKSNPHVKG 262
            I GMGG+GKTTLAK ++N E +   F+   WV VS  F    V +KI+          K 
Sbjct: 199  ITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQKILRDLKPKEEEKKI 258

Query: 263  ME----ALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLLMSGAKGSKILITKRDSK 322
            ME     LQ EL  ++   K L+V+DD+W   +E W  +KP +    KG K+L+T R+  
Sbjct: 259  MEMTQDTLQGELIRLLETSKSLIVLDDIW--EKEDWELIKP-IFPPTKGWKVLLTSRNES 318

Query: 323  VAAEIESMTSLFTLEGLPDGKSWSLFSKVA--FKEGKEVE-NPNLIHLGKEILVKSGGVP 382
            VA    +    F  E L    SW+LF ++A   K+  E + +     LGK ++   GG+P
Sbjct: 319  VAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDEEKEELGKLMIKHCGGLP 378

Query: 383  LVIRHIGRLLYSKTSEEEWMSFKNNELLEIT------QQDNDMTS--VLKLSYNHLPPNL 442
            L IR +G +L  K +  +W     N    +         DN+ T   VL LS+  LP  L
Sbjct: 379  LAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNTCNYVLSLSFEELPSYL 438

Query: 443  KQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSN-GTKCMEDTGNDYFKELCWRFFL-- 502
            K CF YL+ FP    + + +L   W A G  +  +   + + D G+ Y +EL  R  +  
Sbjct: 439  KHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEIIRDVGDVYIEELVRRNMVIS 498

Query: 503  ENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKSWSD 562
            E  +    F+   H+HD+MR++    A ++   +  +   +       V+    +  +  
Sbjct: 499  ERDVKTSRFET-CHLHDMMREVCLLKAKEENFLQITSSRTSTGNSLSIVTSRRLVYQYPI 558

Query: 563  VLS--------KLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNS 622
             L         KLR+   +    +F   + +         +L   F     LRVL ++ +
Sbjct: 559  TLDVEKDINDPKLRSLVVVANTYMFWGGWSW--------MLLGSSFIRLELLRVLDIHRA 618

Query: 623  NSR--TVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKEL-PRDTR 682
              +   + +SI +L HLRYL+L    +  +P S+  L+ L  LNL+       L P   +
Sbjct: 619  KLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYSLGNLKLLIYLNLVILVSGSTLVPNVLK 678

Query: 683  NLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRII 742
             +  LR+L             +  L  L+TL  F     ++  L +L  ++     LR +
Sbjct: 679  EMQQLRYLALPKDMGRKTKLELSNLVKLETLKNF---STKNCSLEDLRGMVR----LRTL 738

Query: 743  GLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVES 802
             +E  +    E    ++  +K L++L +       D   E    E  I+        +++
Sbjct: 739  TIELRKETSLETLAASIGGLKYLESLTI------TDLGSEMRTKEAGIVFDF---VYLKT 798

Query: 803  LRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFID 862
            L +  Y  +   +      S LT + +  C RL+    M  L+ L  L   ++R   F  
Sbjct: 799  LTLKLY--MPRLSKEQHFPSHLTTLYLQHC-RLEE-DPMPILEKLHQLKELELRRKSFSG 858

Query: 863  KNEPSSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMP 922
            K    SS  FP L+ L I  +   E  W++ E+       + P L +L I  C KL  +P
Sbjct: 859  KEMVCSSGGFPQLQKLSIKGLEEWED-WKVEES-------SMPVLHTLDIRDCRKLKQLP 918

Query: 923  K---PAS-TGAVVFLCDVGVQLVSALGPLWGLKSLTL-----------------EEIEDL 982
                P+  T   +F C +    +  L  L  LK L L                  ++  L
Sbjct: 919  DEHLPSHLTSISLFFCCLEEDPMPTLERLVHLKELQLLFRSFSGRIMVCAGSGFPQLHKL 978

Query: 983  KYLEI------MQNLGSLPIQLRHLKIRKCSNLMSLP------------------EWI-- 1030
            K  E+      +   GS+P QL  L+IR+C  L  LP                  EWI  
Sbjct: 979  KLSELDGLEEWIVEDGSMP-QLHTLEIRRCPKLKKLPNGFPQLQNLELNELEEWEEWIVE 1038

BLAST of Lsi02G025580 vs. TAIR 10
Match: AT1G59218.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 245.7 bits (626), Expect = 1.6e-64
Identity = 274/1085 (25.25%), Postives = 466/1085 (42.95%), Query Frame = 0

Query: 23   QRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSHLLENWLQKLEEALHDAEDVLDE 82
            Q   +  G++D + +L   ++ +   L DA+ ++  S +++N +++++E ++D ED ++ 
Sbjct: 19   QECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSAVVKNCVEEIKEIIYDGEDTIET 78

Query: 83   LSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMARQIKKIWERLDAIDAEKKQFHLH 142
               E    +     K+ +++        + AL       I  +  R+  +  + + F + 
Sbjct: 79   FVLEQNLGKTSGIKKSIRRLACIIPDRRRYALG------IGGLSNRISKVIRDMQSFGVQ 138

Query: 143  EN-CESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKKKVKDLLLDVNMNVTHNVSFVA 202
            +   +   +    D+    R  +S  ++ + +G + + KK+   L+D       NV  V+
Sbjct: 139  QAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVGYLVD-----EANVQVVS 198

Query: 203  IVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFEIKAVVEKIIESATKSNPHVKG 262
            I GMGG+GKTTLAK ++N E +   F+   WV VS  F    V +KI+          K 
Sbjct: 199  ITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQKILRDLKPKEEEKKI 258

Query: 263  ME----ALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLLMSGAKGSKILITKRDSK 322
            ME     LQ EL  ++   K L+V+DD+W   +E W  +KP +    KG K+L+T R+  
Sbjct: 259  MEMTQDTLQGELIRLLETSKSLIVLDDIW--EKEDWELIKP-IFPPTKGWKVLLTSRNES 318

Query: 323  VAAEIESMTSLFTLEGLPDGKSWSLFSKVA--FKEGKEVE-NPNLIHLGKEILVKSGGVP 382
            VA    +    F  E L    SW+LF ++A   K+  E + +     LGK ++   GG+P
Sbjct: 319  VAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDEEKEELGKLMIKHCGGLP 378

Query: 383  LVIRHIGRLLYSKTSEEEWMSFKNNELLEIT------QQDNDMTS--VLKLSYNHLPPNL 442
            L IR +G +L  K +  +W     N    +         DN+ T   VL LS+  LP  L
Sbjct: 379  LAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNTCNYVLSLSFEELPSYL 438

Query: 443  KQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSN-GTKCMEDTGNDYFKELCWRFFL-- 502
            K CF YL+ FP    + + +L   W A G  +  +   + + D G+ Y +EL  R  +  
Sbjct: 439  KHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEIIRDVGDVYIEELVRRNMVIS 498

Query: 503  ENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKSWSD 562
            E  +    F+   H+HD+MR++    A ++   +  +   +       V+    +  +  
Sbjct: 499  ERDVKTSRFET-CHLHDMMREVCLLKAKEENFLQITSSRTSTGNSLSIVTSRRLVYQYPI 558

Query: 563  VLS--------KLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNS 622
             L         KLR+   +    +F   + +         +L   F     LRVL ++ +
Sbjct: 559  TLDVEKDINDPKLRSLVVVANTYMFWGGWSW--------MLLGSSFIRLELLRVLDIHRA 618

Query: 623  NSR--TVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKEL-PRDTR 682
              +   + +SI +L HLRYL+L    +  +P S+  L+ L  LNL+       L P   +
Sbjct: 619  KLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYSLGNLKLLIYLNLVILVSGSTLVPNVLK 678

Query: 683  NLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRII 742
             +  LR+L             +  L  L+TL  F     ++  L +L  ++     LR +
Sbjct: 679  EMQQLRYLALPKDMGRKTKLELSNLVKLETLKNF---STKNCSLEDLRGMVR----LRTL 738

Query: 743  GLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVES 802
             +E  +    E    ++  +K L++L +       D   E    E  I+        +++
Sbjct: 739  TIELRKETSLETLAASIGGLKYLESLTI------TDLGSEMRTKEAGIVFDF---VYLKT 798

Query: 803  LRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFID 862
            L +  Y  +   +      S LT + +  C RL+    M  L+ L  L   ++R   F  
Sbjct: 799  LTLKLY--MPRLSKEQHFPSHLTTLYLQHC-RLEE-DPMPILEKLHQLKELELRRKSFSG 858

Query: 863  KNEPSSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMP 922
            K    SS  FP L+ L I  +   E  W++ E+       + P L +L I  C KL  +P
Sbjct: 859  KEMVCSSGGFPQLQKLSIKGLEEWED-WKVEES-------SMPVLHTLDIRDCRKLKQLP 918

Query: 923  K---PAS-TGAVVFLCDVGVQLVSALGPLWGLKSLTL-----------------EEIEDL 982
                P+  T   +F C +    +  L  L  LK L L                  ++  L
Sbjct: 919  DEHLPSHLTSISLFFCCLEEDPMPTLERLVHLKELQLLFRSFSGRIMVCAGSGFPQLHKL 978

Query: 983  KYLEI------MQNLGSLPIQLRHLKIRKCSNLMSLP------------------EWI-- 1030
            K  E+      +   GS+P QL  L+IR+C  L  LP                  EWI  
Sbjct: 979  KLSELDGLEEWIVEDGSMP-QLHTLEIRRCPKLKKLPNGFPQLQNLELNELEEWEEWIVE 1038

BLAST of Lsi02G025580 vs. TAIR 10
Match: AT1G58848.2 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 245.7 bits (626), Expect = 1.6e-64
Identity = 274/1085 (25.25%), Postives = 466/1085 (42.95%), Query Frame = 0

Query: 23   QRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSHLLENWLQKLEEALHDAEDVLDE 82
            Q   +  G++D + +L   ++ +   L DA+ ++  S +++N +++++E ++D ED ++ 
Sbjct: 19   QECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSAVVKNCVEEIKEIIYDGEDTIET 78

Query: 83   LSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMARQIKKIWERLDAIDAEKKQFHLH 142
               E    +     K+ +++        + AL       I  +  R+  +  + + F + 
Sbjct: 79   FVLEQNLGKTSGIKKSIRRLACIIPDRRRYALG------IGGLSNRISKVIRDMQSFGVQ 138

Query: 143  EN-CESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKKKVKDLLLDVNMNVTHNVSFVA 202
            +   +   +    D+    R  +S  ++ + +G + + KK+   L+D       NV  V+
Sbjct: 139  QAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVGYLVD-----EANVQVVS 198

Query: 203  IVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFEIKAVVEKIIESATKSNPHVKG 262
            I GMGG+GKTTLAK ++N E +   F+   WV VS  F    V +KI+          K 
Sbjct: 199  ITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQKILRDLKPKEEEKKI 258

Query: 263  ME----ALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLLMSGAKGSKILITKRDSK 322
            ME     LQ EL  ++   K L+V+DD+W   +E W  +KP +    KG K+L+T R+  
Sbjct: 259  MEMTQDTLQGELIRLLETSKSLIVLDDIW--EKEDWELIKP-IFPPTKGWKVLLTSRNES 318

Query: 323  VAAEIESMTSLFTLEGLPDGKSWSLFSKVA--FKEGKEVE-NPNLIHLGKEILVKSGGVP 382
            VA    +    F  E L    SW+LF ++A   K+  E + +     LGK ++   GG+P
Sbjct: 319  VAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDEEKEELGKLMIKHCGGLP 378

Query: 383  LVIRHIGRLLYSKTSEEEWMSFKNNELLEIT------QQDNDMTS--VLKLSYNHLPPNL 442
            L IR +G +L  K +  +W     N    +         DN+ T   VL LS+  LP  L
Sbjct: 379  LAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNTCNYVLSLSFEELPSYL 438

Query: 443  KQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSN-GTKCMEDTGNDYFKELCWRFFL-- 502
            K CF YL+ FP    + + +L   W A G  +  +   + + D G+ Y +EL  R  +  
Sbjct: 439  KHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEIIRDVGDVYIEELVRRNMVIS 498

Query: 503  ENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKSWSD 562
            E  +    F+   H+HD+MR++    A ++   +  +   +       V+    +  +  
Sbjct: 499  ERDVKTSRFET-CHLHDMMREVCLLKAKEENFLQITSSRTSTGNSLSIVTSRRLVYQYPI 558

Query: 563  VLS--------KLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNS 622
             L         KLR+   +    +F   + +         +L   F     LRVL ++ +
Sbjct: 559  TLDVEKDINDPKLRSLVVVANTYMFWGGWSW--------MLLGSSFIRLELLRVLDIHRA 618

Query: 623  NSR--TVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKEL-PRDTR 682
              +   + +SI +L HLRYL+L    +  +P S+  L+ L  LNL+       L P   +
Sbjct: 619  KLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYSLGNLKLLIYLNLVILVSGSTLVPNVLK 678

Query: 683  NLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRII 742
             +  LR+L             +  L  L+TL  F     ++  L +L  ++     LR +
Sbjct: 679  EMQQLRYLALPKDMGRKTKLELSNLVKLETLKNF---STKNCSLEDLRGMVR----LRTL 738

Query: 743  GLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVES 802
             +E  +    E    ++  +K L++L +       D   E    E  I+        +++
Sbjct: 739  TIELRKETSLETLAASIGGLKYLESLTI------TDLGSEMRTKEAGIVFDF---VYLKT 798

Query: 803  LRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFID 862
            L +  Y  +   +      S LT + +  C RL+    M  L+ L  L   ++R   F  
Sbjct: 799  LTLKLY--MPRLSKEQHFPSHLTTLYLQHC-RLEE-DPMPILEKLHQLKELELRRKSFSG 858

Query: 863  KNEPSSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMP 922
            K    SS  FP L+ L I  +   E  W++ E+       + P L +L I  C KL  +P
Sbjct: 859  KEMVCSSGGFPQLQKLSIKGLEEWED-WKVEES-------SMPVLHTLDIRDCRKLKQLP 918

Query: 923  K---PAS-TGAVVFLCDVGVQLVSALGPLWGLKSLTL-----------------EEIEDL 982
                P+  T   +F C +    +  L  L  LK L L                  ++  L
Sbjct: 919  DEHLPSHLTSISLFFCCLEEDPMPTLERLVHLKELQLLFRSFSGRIMVCAGSGFPQLHKL 978

Query: 983  KYLEI------MQNLGSLPIQLRHLKIRKCSNLMSLP------------------EWI-- 1030
            K  E+      +   GS+P QL  L+IR+C  L  LP                  EWI  
Sbjct: 979  KLSELDGLEEWIVEDGSMP-QLHTLEIRRCPKLKKLPNGFPQLQNLELNELEEWEEWIVE 1038

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XA392.6e-15234.93Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA401.0e-14834.47Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XBQ91.3e-14035.09Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Q7XA421.0e-13733.78Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q9LRR41.3e-11930.42Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A6J1D1680.0e+0068.38putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1GPK90.0e+0057.25putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A1S3CEH40.0e+0058.37disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=... [more]
A0A5A7UWP60.0e+0058.03Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A6J1JVS70.0e+0056.38putative disease resistance protein RGA3 OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
Match NameE-valueIdentityDescription
XP_038897566.10.0e+0089.87putative disease resistance protein RGA4 [Benincasa hispida][more]
XP_031745107.10.0e+0069.43putative disease resistance protein RGA4 [Cucumis sativus][more]
KAE8646395.10.0e+0069.43hypothetical protein Csa_016171 [Cucumis sativus][more]
XP_022147051.10.0e+0068.38putative disease resistance protein RGA4 [Momordica charantia][more]
XP_022953917.10.0e+0057.25putative disease resistance protein RGA4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G14470.19.1e-12130.42NB-ARC domain-containing disease resistance protein [more]
AT3G14460.12.3e-10030.43LRR and NB-ARC domains-containing disease resistance protein [more]
AT1G58848.11.6e-6425.25Disease resistance protein (CC-NBS-LRR class) family [more]
AT1G59218.11.6e-6425.25Disease resistance protein (CC-NBS-LRR class) family [more]
AT1G58848.21.6e-6425.25Disease resistance protein (CC-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 62..86
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 370..384
score: 35.17
coord: 628..644
score: 32.79
coord: 199..214
score: 60.47
coord: 272..286
score: 42.83
NoneNo IPR availableGENE3D1.20.5.4130coord: 10..123
e-value: 1.5E-18
score: 68.9
NoneNo IPR availablePANTHERPTHR23155:SF1055OS05G0414200 PROTEINcoord: 31..988
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 835..1005
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 498..892
IPR003591Leucine-rich repeat, typical subtypeSMARTSM00369LRR_typ_2coord: 607..630
e-value: 0.18
score: 20.9
coord: 654..677
e-value: 5.7
score: 15.3
IPR041118Rx, N-terminalPFAMPF18052Rx_Ncoord: 11..97
e-value: 8.7E-19
score: 67.6
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 419..509
e-value: 2.1E-12
score: 49.0
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 334..418
e-value: 2.3E-12
score: 48.3
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 177..420
e-value: 2.1E-45
score: 154.9
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 535..899
e-value: 2.5E-39
score: 136.6
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 903..1013
e-value: 1.7E-17
score: 64.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 159..329
e-value: 1.2E-37
score: 131.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 163..433
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 585..641
e-value: 7.5E-8
score: 32.0
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 609..630
score: 8.073888
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 31..988

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G025580.1Lsi02G025580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding
molecular_function GO:0005515 protein binding