Homology
BLAST of Lsi02G025580 vs. ExPASy Swiss-Prot
Match:
Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)
HSP 1 Score: 541.2 bits (1393), Expect = 2.6e-152
Identity = 371/1062 (34.93%), Postives = 564/1062 (53.11%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
MAEA L +L L+S +L ++FG + + KL+ STI+ VL DA+ +Q K
Sbjct: 1 MAEAFL----QVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDK 60
Query: 61 LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
+ENWLQKL A ++ +D+L E EA+ E Q R+ F + +++ R
Sbjct: 61 AIENWLQKLNSAAYEVDDILGECKNEAIRFE---------QSRLGFYHPGIINFRHKIGR 120
Query: 121 QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
++K+I E+LDAI E+++FH E + RE+ E +V GRD ++
Sbjct: 121 RMKEIMEKLDAISEERRKFHFLEKITER------QAAAATRETGFVLTEPKVYGRDKEED 180
Query: 181 KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
++ +L++ N+NV + I+GMGG+GKTTLA+ ++NDE+++K+F KIWV VSD F+
Sbjct: 181 EIVKILIN-NVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFD 240
Query: 241 IKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
K +++ II + +S+PHV+ + + Q +LQE++ GK+YLLV+DDVWN+ EKW L+ +L
Sbjct: 241 EKRLIKTIIGNIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVL 300
Query: 301 MSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLI 360
GA+G+ IL T R KV + I + L L S LF + AF + KE NPNL+
Sbjct: 301 TVGARGASILATTRLEKVGS-IMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEA-NPNLV 360
Query: 361 HLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYN 420
+GKEI+ K GGVPL + +G LL K E EW ++NE+ + Q ++ + L+LSY+
Sbjct: 361 AIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESSILPALRLSYH 420
Query: 421 HLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWR 480
HLP +L+QCFAY ++FPK + +LI WMA GF+ S G +ED GN+ + EL R
Sbjct: 421 HLPLDLRQCFAYCAVFPKDTKMIKENLITLWMAHGFL-LSKGNLELEDVGNEVWNELYLR 480
Query: 481 FFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLN----RDYAFNEKTRHVSFDC 540
F + I + + +HD++ DLAT++ C N+ +DY + T + F
Sbjct: 481 SFFQ-EIEAKSGNTYFKIHDLIHDLATSLFSASASCGNIREINVKDY---KHTVSIGFAA 540
Query: 541 KIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSN 600
+ S+S L K F LRVL L+ S
Sbjct: 541 VVSSYSPSLLK-----------------------------------KFVSLRVLNLSYSK 600
Query: 601 SRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVN 660
+P+SI L HLRYLDLS NN SLP + +LQNLQTL++ C L LP+ T L +
Sbjct: 601 LEQLPSSIGDLLHLRYLDLSCNNFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSS 660
Query: 661 LRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEY 720
LRHL + LT P IG L+CL+TL +F++ K+ QL EL L +L G + I LE
Sbjct: 661 LRHLVVDGCPLTSTPPRIGLLTCLKTLGFFIVGSKKGYQLGELKNL-NLCGSISITHLER 720
Query: 721 LRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRID 780
++++ + NL L++L + W +D E E ++E L+PHPN++ L I
Sbjct: 721 VKNDTD--AEANLSAKANLQSLSMSWD---NDGPNRYESKEVKVLEALKPHPNLKYLEII 780
Query: 781 GYSGVGLTNWVSSS-LSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDD-MRSLKFIDKN 840
+ G +W++ S L K+ + I C L G L L L L + +++++++
Sbjct: 781 AFGGFRFPSWINHSVLEKVISVRIKSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEED 840
Query: 841 EP----SSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSL 900
+ S+ SFPSL+ L I +L+G + +K FP L + I CP L +
Sbjct: 841 DVHSRFSTRRSFPSLKKLRIWFFRSLKGLMKEEGEEK------FPMLEEMAILYCP-LFV 900
Query: 901 MPKPASTGAVVFLCDVGVQLVSALGPLWGLKSL-----------------TLEEIEDLKY 960
P +S + + + +S++ L L SL +L +E L +
Sbjct: 901 FPTLSSVKKLEVHGNTNTRGLSSISNLSTLTSLRIGANYRATSLPEEMFTSLTNLEFLSF 960
Query: 961 LEIMQNLGSLPIQ------LRHLKIRKCSNLMSLPE-WIGILTSLEDLEIMECPKLKSLP 1020
+ +NL LP L+ L+I C +L S PE + LTSL L + C LK LP
Sbjct: 961 FD-FKNLKDLPTSLTSLNALKRLQIESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLP 986
Query: 1021 EGMEKLESLRSFCIGGCPEVEERC-KQGGEDWPKIAHIPDFE 1028
EG++ L +L + + GCPEVE+RC K+ GEDW KIAHIP+ +
Sbjct: 1021 EGLQHLTALTNLGVSGCPEVEKRCDKEIGEDWHKIAHIPNLD 986
BLAST of Lsi02G025580 vs. ExPASy Swiss-Prot
Match:
Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)
HSP 1 Score: 529.3 bits (1362), Expect = 1.0e-148
Identity = 364/1056 (34.47%), Postives = 566/1056 (53.60%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
MAEA L +L L+ LG++FG + + KL+ S I+ VL DA+ +Q K
Sbjct: 1 MAEAFL----QVLLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYK 60
Query: 61 LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
++NWLQKL A ++ +D+LD+ TEA KQ + + Y++ +
Sbjct: 61 AIKNWLQKLNVAAYEVDDILDDCKTEA---------ARFKQAVLGRYHPRTITFCYKVGK 120
Query: 121 QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
++K++ E+LDAI E++ FHL E ++ R++ E +V GR+ ++
Sbjct: 121 RMKEMMEKLDAIAEERRNFHLDERI--------IERQAARRQTGFVLTEPKVYGREKEED 180
Query: 181 KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
++ +L++ N++ + V + I+GMGG+GKTTLA+ ++ND++++++F LKIWV VSD F+
Sbjct: 181 EIVKILIN-NVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFD 240
Query: 241 IKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
K +++ I+ES + + LQ +LQE++ GK+Y LV+DDVWNE +EKW L+ +L
Sbjct: 241 EKRLIKAIVESIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVL 300
Query: 301 MSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLI 360
GA G+ ILIT R K+ + I L+ L L W LF + AF E +P L+
Sbjct: 301 KIGASGASILITTRLEKIGS-IMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTET-SPKLM 360
Query: 361 HLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYN 420
+GKEI+ K GGVPL + +G LL K E EW +++E+ + Q +N + L+LSY+
Sbjct: 361 EIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDENSVLPALRLSYH 420
Query: 421 HLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWR 480
HLP +L+QCFAY ++FPK ++ LI WMA F+ S G +ED GN+ + EL R
Sbjct: 421 HLPLDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFL-LSKGNMELEDVGNEVWNELYLR 480
Query: 481 FFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKS 540
F + I + +HD++ DLAT++ ++ + +R + +K
Sbjct: 481 SFFQ-EIEVKSGKTYFKMHDLIHDLATSM-------------FSASASSRSIR-QINVKD 540
Query: 541 WSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTV 600
D++ + K + S F +E+ + +F F LRVL L+NS +
Sbjct: 541 DEDMMFIVTNYK-----DMMSIGF-----SEVVSSYSPSLFKRFVSLRVLNLSNSEFEQL 600
Query: 601 PNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRHL 660
P+S+ L HLRYLDLS N + SLP + +LQNLQTL+L C+ L LP+ T L +LR+L
Sbjct: 601 PSSVGDLVHLRYLDLSGNKICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNL 660
Query: 661 DFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYLRHN 720
+ LT MP IG L+CL+TL YFV+ ++ QL EL L +L G + I LE ++ N
Sbjct: 661 VLDHCPLTSMPPRIGLLTCLKTLGYFVVGERKGYQLGELRNL-NLRGAISITHLERVK-N 720
Query: 721 PSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDGYSG 780
E NL L +L + W D+ E +E ++E L+PHPN++ L I + G
Sbjct: 721 DMEAKEANLSAKANLHSLSMSW-----DRPNRYESEEVKVLEALKPHPNLKYLEIIDFCG 780
Query: 781 VGLTNWVSSS-LSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDD-MRSLKFIDKNEPSS 840
L +W++ S L + I+I C L G L L L L D +++++ + +
Sbjct: 781 FCLPDWMNHSVLKNVVSILISGCENCSCLPPFGELPCLESLELQDGSVEVEYVEDSGFLT 840
Query: 841 SSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMPKPASTG 900
FPSL L I NL+G + ++ FP L + IS CP + + P +S
Sbjct: 841 RRRFPSLRKLHIGGFCNLKGLQRMKGAEQ------FPVLEEMKISDCP-MFVFPTLSSVK 900
Query: 901 AVVFLCDV---GVQLVSALGPLWGLKSLT-------LEE----IEDLKYLEI--MQNLGS 960
+ + G+ +S L L LK + LEE +E+L YL + ++NL
Sbjct: 901 KLEIWGEADAGGLSSISNLSTLTSLKIFSNHTVTSLLEEMFKNLENLIYLSVSFLENLKE 960
Query: 961 LPI------QLRHLKIRKCSNLMSLP-EWIGILTSLEDLEIMECPKLKSLPEGMEKLESL 1020
LP L+ L IR C L SLP E + L+SL +L + C LK LPEG++ L +L
Sbjct: 961 LPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTL 992
Query: 1021 RSFCIGGCPEVEERCKQG-GEDWPKIAHIPDFEHFL 1031
S I GCP++ +RC++G GEDW KI+HIP+ ++
Sbjct: 1021 TSLKIRGCPQLIKRCEKGIGEDWHKISHIPNVNIYI 992
BLAST of Lsi02G025580 vs. ExPASy Swiss-Prot
Match:
Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)
HSP 1 Score: 502.3 bits (1292), Expect = 1.3e-140
Identity = 367/1046 (35.09%), Postives = 565/1046 (54.02%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
MAEA + +L L+S L +LFG +D+ +L+ STI+ VL DA+ +Q +
Sbjct: 1 MAEAFI----QVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNK 60
Query: 61 LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
LENWLQKL A ++ +D+LDE T+A TR ++ R + +++ +
Sbjct: 61 PLENWLQKLNAATYEVDDILDEYKTKA------TRFSQSEYGRY---HPKVIPFRHKVGK 120
Query: 121 QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
++ ++ ++L AI E+K FHLHE ++ RE+ S E +V GRD +K
Sbjct: 121 RMDQVMKKLKAIAEERKNFHLHEKI--------VERQAVRRETGSVLTEPQVYGRDKEKD 180
Query: 181 KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
++ +L++ N++ ++S + I+GMGG+GKTTLA+ ++ND++++++F KIW+ VS+ F+
Sbjct: 181 EIVKILIN-NVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFD 240
Query: 241 IKAVVEKIIESATKSNPHVKGME--ALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKP 300
K +++ I+ES + P + M+ LQ +LQE++ GK+YLLV+DDVWNE ++KW L+
Sbjct: 241 EKRLIKAIVES-IEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRA 300
Query: 301 LLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPN 360
+L GA G+ +L T R KV + I + L L W LF + AF +E+ NPN
Sbjct: 301 VLKVGASGASVLTTTRLEKVGS-IMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEI-NPN 360
Query: 361 LIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLS 420
L+ +GKEI+ KSGGVPL + +G +L K E W +++ + + Q ++ + L+LS
Sbjct: 361 LVAIGKEIVKKSGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESSILPALRLS 420
Query: 421 YNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELC 480
Y+ LP +LKQCFAY ++FPK ++ LI WMA GF+ S G +ED G++ +KEL
Sbjct: 421 YHQLPLDLKQCFAYCAVFPKDAKMEKEKLISLWMAHGFL-LSKGNMELEDVGDEVWKELY 480
Query: 481 WRFFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKI 540
R F + I + +HD++ DLAT++ N+ E +H
Sbjct: 481 LRSFFQ-EIEVKDGKTYFKMHDLIHDLATSLFSANTSSSNI------REINKH------- 540
Query: 541 KSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSR 600
S++ ++S G F + EK F LRVL L +S
Sbjct: 541 -SYTHMMS-----IGFAEVVFFYTLPPLEK---------------FISLRVLNLGDSTFN 600
Query: 601 TVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLR 660
+P+SI L HLRYL+L + M SLP + +LQNLQTL+L YC +L LP++T L +LR
Sbjct: 601 KLPSSIGDLVHLRYLNLYGSGMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLR 660
Query: 661 HLDFNTS-TLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYL 720
+L + S +LT MP IG L+CL+TL FV+ K+ QL EL L +L G ++I LE +
Sbjct: 661 NLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQLGELGNL-NLYGSIKISHLERV 720
Query: 721 RHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDG 780
+ N + NL L +L + W + YE E E ++E L+PH N+ SL+I G
Sbjct: 721 K-NDKDAKEANLSAKGNLHSLSMSWN-NFGPHIYESE--EVKVLEALKPHSNLTSLKIYG 780
Query: 781 YSGVGLTNWVSSS-LSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLD----DMRSLKFID 840
+ G+ L W++ S L + I+I L G L L L L D+ ++ +D
Sbjct: 781 FRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVD 840
Query: 841 KNEPSSSSS---FPSLELLIIANMPNLEGWWEL-GETQKNWLPPTFPKLISLHISGCPKL 900
+ S + FPSL L I + +L+G + GE Q FP L + I CP L
Sbjct: 841 IDVHSGFPTRIRFPSLRKLDIWDFGSLKGLLKKEGEEQ-------FPVLEEMIIHECPFL 900
Query: 901 SLMPK-PASTGAVVFLCDVGVQLVSAL-GPLWGLKSLTLEEIEDLKYLEIMQNLGSLPIQ 960
+L A T + V + L LK LT+ +LK E+ +L SL
Sbjct: 901 TLSSNLRALTSLRICYNKVATSFPEEMFKNLANLKYLTISRCNNLK--ELPTSLASLN-A 960
Query: 961 LRHLKIRKCSNLMSLP-EWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSFCIGGCPE 1020
L+ LKI+ C L SLP E + L+SL +L + C LK LPEG++ L +L S I GCP+
Sbjct: 961 LKSLKIQLCCALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQ 970
Query: 1021 VEERCKQG-GEDWPKIAHIPDFEHFL 1031
+ +RC++G GEDW KI+HIP+ ++
Sbjct: 1021 LIKRCEKGIGEDWHKISHIPNVNIYI 970
BLAST of Lsi02G025580 vs. ExPASy Swiss-Prot
Match:
Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)
HSP 1 Score: 492.7 bits (1267), Expect = 1.0e-137
Identity = 355/1051 (33.78%), Postives = 551/1051 (52.43%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
MAEA + +L L+S L +LFG +D+ +L+ STI+ VL DA+ +Q
Sbjct: 1 MAEAFI----QVVLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDK 60
Query: 61 LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
LENWLQKL A ++ +D+LDE T+A TR ++ R + +++ +
Sbjct: 61 PLENWLQKLNAATYEVDDILDEYKTKA------TRFLQSEYGRY---HPKVIPFRHKVGK 120
Query: 121 QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
++ ++ ++L+AI E+K+FHL E ++ RE+ S E +V GRD +K
Sbjct: 121 RMDQVMKKLNAIAEERKKFHLQEKI--------IERQAATRETGSVLTEPQVYGRDKEKD 180
Query: 181 KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
++ +L++ + +S + I+GMGG+GKTTL++ ++ND+++++ F KIW+ +SD F
Sbjct: 181 EIVKILINTASD-AQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFN 240
Query: 241 IKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
K +++ I+ES + + LQ +LQE++ GK+Y LV+DDVWNE + KW L+ +L
Sbjct: 241 EKRLIKAIVESIEGKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVL 300
Query: 301 MSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLI 360
GA G+ +L T R KV + I + L L W LF + AF +E+ NPNL+
Sbjct: 301 KVGASGAFVLTTTRLEKVGS-IMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEI-NPNLM 360
Query: 361 HLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYN 420
+GKEI+ K GGVPL + +G +L K E EW +++ + + Q ++ + L+LSY+
Sbjct: 361 AIGKEIVKKCGGVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSILPALRLSYH 420
Query: 421 HLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWR 480
HLP +L+QCF Y ++FPK + +LI WMA GF+ S G +ED GN+ + EL R
Sbjct: 421 HLPLDLRQCFVYCAVFPKDTKMAKENLIAFWMAHGFL-LSKGNLELEDVGNEVWNELYLR 480
Query: 481 FFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKS 540
F + I + +HD++ DLAT++ F S
Sbjct: 481 SFFQ-EIEVESGKTYFKMHDLIHDLATSL------------------------FSANTSS 540
Query: 541 WSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTV 600
S +R + S F E+ + + F LRVL L NSN +
Sbjct: 541 -----SNIREINANYDGYMMSIGFA-----EVVSSYSPSLLQKFVSLRVLNLRNSNLNQL 600
Query: 601 PNSIRRLRHLRYLDLSEN-NMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRH 660
P+SI L HLRYLDLS N + +LP + +LQNLQTL+L YC L LP+ T L +LR+
Sbjct: 601 PSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRN 660
Query: 661 LDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYLRH 720
L + +LT P IG L+CL++LS FV+ ++ QL EL L +L G + I L+ ++
Sbjct: 661 LLLDGCSLTSTPPRIGLLTCLKSLSCFVIGKRKGHQLGELKNL-NLYGSISITKLDRVKK 720
Query: 721 NPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDGYS 780
+ ++ NL L +L L W L Y+ E ++E L+PH N++ L I+G+
Sbjct: 721 D-TDAKEANLSAKANLHSLCLSWDLD-GKHRYDSE-----VLEALKPHSNLKYLEINGFG 780
Query: 781 GVGLTNWVSSS-LSKLTKIIIYKCHRLQHLTQMGHLQALTFLYL-DDMRSLKFIDKNEPS 840
G+ L +W++ S L + I I C L G L L L L +++++ N
Sbjct: 781 GIRLPDWMNQSVLKNVVSIRIRGCENCSCLPPFGELPCLESLELHTGSADVEYVEDN--V 840
Query: 841 SSSSFPSLELLIIANMPNLEGWWEL-GETQKNWLPPTFPKLISLHISGCPKLSLMPKPAS 900
FPSL L+I + NL+G ++ GE Q FP L + CP + ++P +S
Sbjct: 841 HPGRFPSLRKLVIWDFSNLKGLLKMEGEKQ-------FPVLEEMTFYWCP-MFVIPTLSS 900
Query: 901 --------TGAVVFLCDVGVQLVSALGPLWGLKSLTLEE-----IEDLKYLEI--MQNLG 960
T A V ++ +++L +++ +L E + +LKYL+I +NL
Sbjct: 901 VKTLKVIVTDATVLRSISNLRALTSLDISDNVEATSLPEEMFKSLANLKYLKISFFRNLK 960
Query: 961 SLPIQ------LRHLKIRKCSNLMSLP-EWIGILTSLEDLEIMECPKLKSLPEGMEKLES 1020
LP L+ LK C L SLP E + LTSL +L + C LK LPEG++ L +
Sbjct: 961 ELPTSLASLNALKSLKFEFCDALESLPEEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTA 973
Query: 1021 LRSFCIGGCPEVEERCKQG-GEDWPKIAHIP 1025
L + I CP V +RC++G GEDW KIAHIP
Sbjct: 1021 LTTLTITQCPIVFKRCERGIGEDWHKIAHIP 973
BLAST of Lsi02G025580 vs. ExPASy Swiss-Prot
Match:
Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 432.6 bits (1111), Expect = 1.3e-119
Identity = 313/1029 (30.42%), Postives = 518/1029 (50.34%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDD-LNKLTGTVSTIKDVLLDAEGRQTKS 60
+ E L A+ L S+ F+ L ++ L +L+ + TI VL+DAE +Q +
Sbjct: 4 IGEMFLAAFLQALFQTLVSEPFRSFFKRRELNENLLERLSTALLTITAVLIDAEEKQITN 63
Query: 61 HLLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNA---KQVRIFFSKSNQLALN- 120
++E W+ +L + ++ AED LD+++TEAL + ++ +Q+R S + L N
Sbjct: 64 PVVEKWVNELRDVVYHAEDALDDIATEALRLNIGAESSSSNRLRQLRGRMSLGDFLDGNS 123
Query: 121 YRMARQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGR 180
+ +++K+ RL+ + +++ L E T ++ T S +E EV GR
Sbjct: 124 EHLETRLEKVTIRLERLASQRNILGLKE----LTAMIPKQRLPT----TSLVDESEVFGR 183
Query: 181 DDDKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWV 240
DDDK ++ L+ N + ++ VAIVG+GG+GKTTL++ LYND+ + YF K+W V
Sbjct: 184 DDDKDEIMRFLIPEN-GKDNGITVVAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHV 243
Query: 241 SDQFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGK--KYLLVMDDVWNESEEKW 300
S++F++ + +K+ ES T ++ LQ +L+E + G +LLV+DD+WNE+ W
Sbjct: 244 SEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLTGTGLPFLLVLDDLWNENFADW 303
Query: 301 HGLKPLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKE 360
L+ + A+GS+IL+T R +VA+ I + L+ L DG WSLF K F +
Sbjct: 304 DLLRQPFIHAAQGSQILVTTRSQRVAS-IMCAVHVHNLQPLSDGDCWSLFMKTVFGNQEP 363
Query: 361 VENPNLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTS 420
N + L + I+ K G+PL ++ +G +L + EW ++ + ++ +++
Sbjct: 364 CLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADKSNLLP 423
Query: 421 VLKLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDY 480
VL++SY +LP +LK+CFAY S+FPKG + + + ++ WMA GF++ + +K +E+ GN+Y
Sbjct: 424 VLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEY 483
Query: 481 FKELCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVS 540
F EL R L+ + +HD + +LA +G+ +E+TR++S
Sbjct: 484 FSELESRSLLQKT------KTRYIMHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLS 543
Query: 541 FDCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLN 600
+ + LR K LRTF L S + +++ + +++ + RLRVL L+
Sbjct: 544 YLRDNYAEPMEFEALREVKFLRTF-LPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLS 603
Query: 601 NSN-SRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTR 660
+ +R P+ + + H R+LDLS +E LP S+ + NLQTL L YC LKELP D
Sbjct: 604 HYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDIS 663
Query: 661 NLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRII 720
NL+NLR+LD + L MP GRL LQTL+ F + S++SEL L L+G L+I+
Sbjct: 664 NLINLRYLDLIGTKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIV 723
Query: 721 GLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGE----DDETAIMEGLEPHP 780
L+ + + ++ + NL K L+ + W+ E +E + E L PH
Sbjct: 724 ELQRV-VDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHR 783
Query: 781 NVESLRIDGYSGVGLTNWVSS-SLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRS 840
++E L I+ Y G +W+S S S++ I + +C L +G L L L++ M
Sbjct: 784 HIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVG 843
Query: 841 LKFIDKN--------EPSSSSSFPSLELLIIANMPNLEGWWELGETQKNWLP-------- 900
L+ I + F SLE L N+P+ + W ++ T+ + P
Sbjct: 844 LQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFIL 903
Query: 901 ---------PTF-PKLISLHISGCPKLSLMP-------KPASTGAVVFLCDVGVQLVSAL 960
PTF P LISLHI C L P + T ++ CD V+ L
Sbjct: 904 RCPELTGTLPTFLPSLISLHIYKCGLLDFQPDHHEYSYRNLQTLSIKSSCDTLVKF--PL 963
Query: 961 GPLWGLKSLTLEEIEDLKYLEIMQNLGSLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDL 984
L L +++ L LE+ P LR+L+I C NL LP+ + +L+ +
Sbjct: 964 NHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLPKLNALPQNLQ-V 1011
BLAST of Lsi02G025580 vs. ExPASy TrEMBL
Match:
A0A6J1D168 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111016078 PE=4 SV=1)
HSP 1 Score: 1351.3 bits (3496), Expect = 0.0e+00
Identity = 720/1053 (68.38%), Postives = 836/1053 (79.39%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGR-QTKS 60
MAE +LF VAG ILMKL S A QRLGM+ G+++DL KLT TVS IK+VLLDAE QTK+
Sbjct: 1 MAEGVLFHVAGEILMKLGSIASQRLGMVRGVENDLTKLTSTVSVIKNVLLDAEEHCQTKT 60
Query: 61 -HLLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRM 120
H L++WLQKLEEAL+DAEDVLDELS+E LHREV+T+ KNAKQVRIFFS SNQ+A NYR+
Sbjct: 61 DHTLQDWLQKLEEALYDAEDVLDELSSETLHREVVTKGKNAKQVRIFFSGSNQIAFNYRV 120
Query: 121 ARQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGR-ESWSFSNEEEVIGRDD 180
AR IKKIWERLDAIDAE+KQF+L ENC+ +T YGSFDQIM GR E+ SF N EEVIGRD
Sbjct: 121 ARNIKKIWERLDAIDAERKQFNLTENCQQRTLYGSFDQIMMGRKETCSFPNVEEVIGRDY 180
Query: 181 DKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSD 240
DKKK+KDLLLD+NMNV HNVSF+ I GMGGIGKTTLAKSLY+D ++S+ F+ IW+W+SD
Sbjct: 181 DKKKLKDLLLDINMNVRHNVSFIVIAGMGGIGKTTLAKSLYHDREVSECFDSTIWIWISD 240
Query: 241 QFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLK 300
QFE++ VVEKIIESATK P+V+GMEAL +ELQEVIGGKKYLLVMDDVW E+EEKWH LK
Sbjct: 241 QFEVRVVVEKIIESATKRKPNVEGMEALNSELQEVIGGKKYLLVMDDVWKETEEKWHELK 300
Query: 301 PLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENP 360
PLLM GA GSKILITKRDSKVA EIESMTSLFTL+GL + SWSLF KVAFKE K+ N
Sbjct: 301 PLLMGGASGSKILITKRDSKVATEIESMTSLFTLKGLQESNSWSLFRKVAFKEAKDPANS 360
Query: 361 NLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQ---DNDMTSV 420
NLI LGKEIL K GGVPLVIRHIGRLLYSKTSEEEWMSFKNNELL++ QQ ++ +T +
Sbjct: 361 NLIKLGKEILAKCGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLKVIQQTDKNDGLTPI 420
Query: 421 LKLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGT-KCMEDTGNDY 480
L LSYNHLPPNLKQCFAYLSLFPKG L+I DLIRQWMA GFIESSNGT ME+ G Y
Sbjct: 421 LNLSYNHLPPNLKQCFAYLSLFPKGTRLEIKDLIRQWMAHGFIESSNGTANSMEEVGKSY 480
Query: 481 FKELCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVS 540
FKELCWRFF ENS +ECNFD+DV +HDVMR++A NVAG+KYV NLN D+ +EKTRH+
Sbjct: 481 FKELCWRFFFENSSDECNFDDDVDMHDVMREVAMNVAGEKYVHGNLNCDFVVSEKTRHIL 540
Query: 541 FDCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEIN-EAILDEVFSSFPRLRVLGL 600
FD KIK W DVL KL+ AKGLRTF LF S F ++ + E N EA+L+E+ SSFPRLRVLGL
Sbjct: 541 FDSKIKLWQDVLLKLQKAKGLRTF-LFLSYFPFKNRLEDNIEAVLEELCSSFPRLRVLGL 600
Query: 601 NNSNSRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTR 660
N+S VPNSI+ LRHLRYL+LS N MESLP SIT+LQNLQ+L+L C +L ELPRDT
Sbjct: 601 NHSKIHVVPNSIKNLRHLRYLNLSRNYMESLPTSITKLQNLQSLDLKDCYKLNELPRDTS 660
Query: 661 NLVNLRHLDF--NTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLR 720
NLVNLRHL + T HM EG+ +LSCL+TL YFVLDCKRS+ SELNV + NG L+
Sbjct: 661 NLVNLRHLVLPDHLDTPLHMLEGMRKLSCLRTLGYFVLDCKRSNMQSELNVPNYFNGRLK 720
Query: 721 IIGLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLS-RDDQEYEGEDDETAIMEGLEPHPN 780
I+GLE LR PS+V LVNLKDI+GL++L+L WKLS RDD E EG+ DE +MEGL+P N
Sbjct: 721 IVGLEQLRRTPSKVGLVNLKDIQGLRDLKLVWKLSGRDDDEGEGKGDE-RVMEGLQPQSN 780
Query: 781 VESLRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLK 840
VESLRI+GYSGVGL NWVS+SLS LTKI I CHR+QHL Q+ HLQ L ++L+DMRSLK
Sbjct: 781 VESLRIEGYSGVGLPNWVSTSLSNLTKIAICNCHRVQHLPQLAHLQTLKGIFLEDMRSLK 840
Query: 841 FIDKNEPSSSSSFPSLELLIIANMPNLEGWWE---LGETQKNWLPPTFPKLISLHISGCP 900
F+DKNEP FPSL L + NM NLEGWWE +GET P FP+L L I CP
Sbjct: 841 FVDKNEP-GLLCFPSLVSLRLKNMGNLEGWWESRVVGETL-----PIFPELQYLRIQHCP 900
Query: 901 KLSLMPKPA-STGAVVFLCDVGVQLVSALGPLWGLKSLTLEEIEDLKYLEI------MQN 960
KLSLMPK A ST FLCDVGVQ+V+A+GPLW + + L+EI+DLK LE+ Q+
Sbjct: 901 KLSLMPKIASSTLDSFFLCDVGVQVVTAIGPLWNFEVVILQEIKDLKDLEMESHQNRNQD 960
Query: 961 LGS--------LPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEK 1020
LGS P +LR L I KCSNLMSLPEWIG +TSL + I++CPKLKSL EGM +
Sbjct: 961 LGSSSLSMSSRSPTRLRFLTIEKCSNLMSLPEWIGTITSLWKVTIIDCPKLKSLLEGMRQ 1020
Query: 1021 LESLRSFCIGGCPEVEERCKQGGEDWPKIAHIP 1025
L+SL+ I GCPE+EERCK+GGEDWP IAHIP
Sbjct: 1021 LKSLKYLTIKGCPELEERCKEGGEDWPNIAHIP 1045
BLAST of Lsi02G025580 vs. ExPASy TrEMBL
Match:
A0A6J1GPK9 (putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111456321 PE=4 SV=1)
HSP 1 Score: 1151.0 bits (2976), Expect = 0.0e+00
Identity = 636/1111 (57.25%), Postives = 782/1111 (70.39%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
MAE +LF++A I+ KLSS A Q+LGML+G+KD+L +L +VS IK V+LDAE RQTKSH
Sbjct: 1 MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEERQTKSH 60
Query: 61 LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
L++WL+KL+EAL+DAEDVLDE+S EA+ R + AKQVRIFFS+ N +A +Y+M R
Sbjct: 61 FLKDWLEKLKEALYDAEDVLDEISGEAVR-----RGRKAKQVRIFFSRYNPIAFDYKMTR 120
Query: 121 QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
QIK + ERL I AE+K FHL E KT++GSFDQI G E+ SFSN EEVIGRDD K+
Sbjct: 121 QIKNVRERLGVIAAERKNFHLTEIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKR 180
Query: 181 KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
+KDLL D MNV N+SF+AIVGM GIGKTTLA+SL+ND ++S+ F+LKIWVWVS FE
Sbjct: 181 VIKDLLQD--MNVKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFE 240
Query: 241 IKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
+K VVEKIIESAT S P+V GME+LQT+LQ+++ GKKYLLVMDD+WNESE+KW LK LL
Sbjct: 241 VKIVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLL 300
Query: 301 MSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLI 360
M GA+GSKI+ITKRDS +A EI +MTSL TL+GL + SWSLF KVAF +G E ENP LI
Sbjct: 301 MGGARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLI 360
Query: 361 HLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYN 420
LGKEILVK GGVPLVIRHIGRLLY + SEEEW+SFKNN+LLE+ Q+D+DM S+LKLSYN
Sbjct: 361 QLGKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEVIQRDHDMASILKLSYN 420
Query: 421 HLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWR 480
HLP NLKQCFAY SL K + + ++LIRQW+A GF++S NG K MEDTGNDYF ELCWR
Sbjct: 421 HLPSNLKQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLNGCKSMEDTGNDYFMELCWR 480
Query: 481 FFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKS 540
FF EN +E NF++DV++HDVM DLA VAG +YV N N D+ E TRH+ FD +I
Sbjct: 481 FFYENFNDEHNFEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVGESTRHILFDREIGI 540
Query: 541 WSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTV 600
W V SKLR AKGLRTF L SN ++KN++NE +LDE+F F RLRVL L SN + V
Sbjct: 541 WEHVPSKLRKAKGLRTFIL--SNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRV 600
Query: 601 PNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRHL 660
P+SI+RL HLRYLDLS N M+SLPNS+T L NLQTLNL C LK+LPR TRNLVNLRHL
Sbjct: 601 PDSIKRLTHLRYLDLSGNYMKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRHL 660
Query: 661 DFNTST-LTHMPEGIGRLSCLQTLSYFVLDC---KRSSQLSELNVLIHLNGDLRIIGLEY 720
+ + ST LTHMPEG+G+L+CLQTLS FVLD + S+L ELN L L G L IIGLE
Sbjct: 661 NISDSTHLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLEN 720
Query: 721 LRHNPSEVSLVNLKDIKGLKNLQLEWK-LSRDDQEYEGEDDETAIMEGLEPHPNVESLRI 780
L+ PSE SL NLK+ KGL++L L WK LS D+ ++ +++ I+ GLEP+P +ES RI
Sbjct: 721 LKFTPSEASLANLKEKKGLRHLTLIWKNLSGDEHRFQADEE---ILAGLEPNPAIESFRI 780
Query: 781 DGYSGVGLTNW--VSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDK 840
GY GV L NW +SS L KLT+I+I C RLQ+L Q+ HL AL LYL ++ SLKFID
Sbjct: 781 SGYFGVKLPNWLSLSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKFIDN 840
Query: 841 NEP-SSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMP 900
+P SS+ FPSLE L + +MPNLE WWE + PTFP L L IS CPKL+ MP
Sbjct: 841 TDPYSSALFFPSLESLCLMDMPNLEAWWERESMVEEEFLPTFPCLTILSISNCPKLATMP 900
Query: 901 -KPASTGAVVFLCDVGVQLVSAL------GPLWGLKSLTLEEIEDLKYL--EIMQNLG-- 960
P T + L VG+Q++S++ P LKSL L+ IEDLK L ++ QNL
Sbjct: 901 GHPPLTD--ITLDGVGLQMMSSIRSSSNTSPSSSLKSLELKGIEDLKNLSHKLRQNLNSL 960
Query: 961 --------------------------------------------------------SLPI 1020
SLPI
Sbjct: 961 KFLYITECESLVNSLFDETRSSSTSTRNYTELGDAMQWKFLHNLKFLRLENLPKLVSLPI 1020
Query: 1021 QLRH------LKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSFC 1031
+++H L I KC N SLPEWIG + SL++LEI+ECPKLKSLPEG+ +L++L
Sbjct: 1021 EVQHWTALKFLIISKCPNFRSLPEWIGGMVSLQELEILECPKLKSLPEGIRELKALNHLR 1080
BLAST of Lsi02G025580 vs. ExPASy TrEMBL
Match:
A0A1S3CEH4 (disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=1)
HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 617/1057 (58.37%), Postives = 769/1057 (72.75%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
MAE IL +AG IL KL S + GML GLKDDL++L TVS I+ L+DA+ T
Sbjct: 255 MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNLPTGLD 314
Query: 61 LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
+ WL+KL LHDAED++DE E HREVMTR+K+AK+VRIFFS SNQLA NY+MAR
Sbjct: 315 SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 374
Query: 121 QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFD---QIMTGRESWSFSNEEEVIGRDD 180
++K+I ERLD ID EK H + + + ++ RE+ S N+E VIGRDD
Sbjct: 375 RVKRIRERLDVIDKEKSYLWSHSFVQGRQQDVPSPIRMRMSLSRETHSSLNKERVIGRDD 434
Query: 181 DKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSD 240
DK+ +K LLL +M++ NVS +AIVGMGGIGKTTLAKSLYND +SK+FE+K+W+WVS
Sbjct: 435 DKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSKHFEIKMWIWVSK 494
Query: 241 QFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLK 300
QF K VV+KI+ESATK P + ++ +Q++LQ+VIGGKKYLLVMD++ NESE++W LK
Sbjct: 495 QFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKKLK 554
Query: 301 PLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEG-KEVEN 360
LL+ G +GSKI+ITKRD KVA+EIE MT+ TL+GL + +SWSLF +VAF+ N
Sbjct: 555 DLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDRSWSLFREVAFEGNLPNPIN 614
Query: 361 PNLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLK 420
P L +GKEI + GGVPL IRH+GR L SK EEEWMS +++LLE+TQQ+ND+ S+L+
Sbjct: 615 PKLELMGKEISSRCGGVPLAIRHMGRFLCSKRCEEEWMSSLSDKLLEVTQQNNDVASILE 674
Query: 421 LSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKE 480
LSYN LP +LKQCFAY SLFPK LK+N+LIRQW+A GFIESS G K ME G +YF E
Sbjct: 675 LSYNDLPLHLKQCFAYSSLFPKVHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYFDE 734
Query: 481 LCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKK-YVCENLNRDYAFNEKTRHVSFD 540
LCWRFF ENS +E NFD+ V++HDVM +LA VA KK YVC ++N +Y +E+T H SFD
Sbjct: 735 LCWRFFYENSSDENNFDDYVYMHDVMYELARKVADKKLYVCGDINNNYVVSEQTLHFSFD 794
Query: 541 CKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNS 600
KI+SW DVLSKL AKGLRTF S YE+KNE+NEA LDE+FSSFPRLRVL L S
Sbjct: 795 YKIQSWQDVLSKLCKAKGLRTFMFLHS--PYEEKNEVNEANLDELFSSFPRLRVLLLGGS 854
Query: 601 NSRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLV 660
N +PNSI++L+ LRYLDLSENNM+SLPNSITELQ+LQTL L C LKE+PRD LV
Sbjct: 855 NIHVLPNSIKKLKLLRYLDLSENNMKSLPNSITELQDLQTLKLTGCYALKEMPRDINKLV 914
Query: 661 NLRHLDFN-TSTLTHM-PEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIG 720
NLRHLD S +THM PEG+ +LSCLQT+S FV DC ++++L ELN L +L G+L+I G
Sbjct: 915 NLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFVFDCTKTNKLRELNELNNLTGELKITG 974
Query: 721 LEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESL 780
L+ LR P EVSLVNLKD K L++L+LEWK+ ++E E DE +M+GLEP+PNVESL
Sbjct: 975 LQQLRSTPYEVSLVNLKDKKCLRSLKLEWKMGL--YKHEDEADE-RVMKGLEPNPNVESL 1034
Query: 781 RIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDK 840
I GY+GVGL W+ + KLT+I I CHRLQHL Q HLQ L L L D+RSLKFIDK
Sbjct: 1035 GIKGYNGVGLPKWMFNCHLKLTEIEIVSCHRLQHLPQFHHLQTLKTLCLRDLRSLKFIDK 1094
Query: 841 --NEPSSSSSFPSLELLIIANMPNLEGWWEL-----GETQKN--WLPPTFPKLISLHISG 900
N SSS FPSL+ L +ANM NLEGWWEL GET +N WLPPTFP+L L I
Sbjct: 1095 FDNPFSSSMFFPSLKSLCLANMANLEGWWELSRAVAGETSENIQWLPPTFPELEILDIYQ 1154
Query: 901 CPKLSLMPK-PASTGAVVFLCDVGVQLV-SALGP-LWGLKSLTLEEIEDLKYLEIMQNLG 960
CPKL+ MPK P+ST A V LCDVGVQLV S +GP L L++L LEEI++L L QN+
Sbjct: 1155 CPKLNSMPKLPSSTKAYVSLCDVGVQLVISTIGPVLLCLRTLRLEEIKNLNCLPFQQNIN 1214
Query: 961 ---------SLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKL 1020
+ PI L++L++ KC +L++LPEWI I TSLE L I +CPKLKSLP+GM++L
Sbjct: 1215 HSLVISSTTTSPIPLKYLQMDKCLDLVTLPEWIYIFTSLETLSISKCPKLKSLPKGMQRL 1274
Query: 1021 ESLRSFCIGGCPEVEERCKQGGEDWPKIAHIPDFEHF 1030
ESL+ CI CP +EERCK+GGEDWP I+H+P+ F
Sbjct: 1275 ESLKRLCIEDCPRLEERCKEGGEDWPNISHVPNIVFF 1306
BLAST of Lsi02G025580 vs. ExPASy TrEMBL
Match:
A0A5A7UWP6 (Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00750 PE=4 SV=1)
HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 614/1058 (58.03%), Postives = 765/1058 (72.31%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
MAE IL +AG IL KL S + GML GLKDDL++L TVS I+ L+DA+ T
Sbjct: 1 MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNWPTGLD 60
Query: 61 LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
+ WL+KL LHDAED++DE E HREVMTR+K+AK+VRIFFS SNQLA NY+MAR
Sbjct: 61 SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 120
Query: 121 QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFD---QIMTGRESWSFSNEEEVIGRDD 180
++K+I ERLD ID EK H + + + ++ + RE+ S N+E VIGRDD
Sbjct: 121 RVKRIRERLDVIDKEKSYLWSHSFVQGRQQDVPSPIRMRMSSSRETHSSLNKERVIGRDD 180
Query: 181 DKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSD 240
DK+ +K LLL +M++ NVS +AIVGMGGIGKTTLAKSLYND +S++FE+K+W+WVS
Sbjct: 181 DKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSEHFEIKMWIWVSK 240
Query: 241 QFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLK 300
QF K VV+KI+ESATK P + ++ +Q++LQ+VIGGKKYLLVMD++ NESE++W LK
Sbjct: 241 QFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKKLK 300
Query: 301 PLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEG-KEVEN 360
LL+ G +GSKI+ITKRD KVA+EIE MT+ TL+GL + SWSLF +VAF+E + N
Sbjct: 301 DLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDSSWSLFREVAFEENLPKPIN 360
Query: 361 PNLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKN-NELLEITQQDNDMTSVL 420
P L +GKEI GGVPL IRH+GR L SK EEEWMS + + LE+ QQ+ND+ S+L
Sbjct: 361 PKLELMGKEISSICGGVPLAIRHMGRFLCSKRCEEEWMSSLSWKKHLEVAQQNNDVASIL 420
Query: 421 KLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFK 480
+LSYN LP +LKQCFAY SLFPKG LK+N+LIRQW+A GFIESS G K ME G +YF
Sbjct: 421 ELSYNDLPLHLKQCFAYSSLFPKGHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYFD 480
Query: 481 ELCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKK-YVCENLNRDYAFNEKTRHVSF 540
ELCWRFF ENS +E NFD+ V++HDVM +LA VA KK +VC ++N +Y +E+T H S
Sbjct: 481 ELCWRFFYENSSDENNFDDYVYMHDVMYELARKVADKKLFVCGDINNNYVVSEQTLHFSL 540
Query: 541 DCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNN 600
D KI+SW DVLSKL AKGLRTF S YE+KNE+NEA LDE+FSSFPRLRVL L
Sbjct: 541 DYKIQSWQDVLSKLCKAKGLRTFMFLHS--PYEEKNEVNEANLDELFSSFPRLRVLLLGG 600
Query: 601 SNSRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNL 660
SN +PNSI++L+ LRYLDLSENNM+SLPNSITELQ+LQTL L C LKE+PRD L
Sbjct: 601 SNIHVLPNSIKKLKLLRYLDLSENNMKSLPNSITELQDLQTLKLTGCYALKEMPRDINKL 660
Query: 661 VNLRHLDFN-TSTLTHM-PEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRII 720
VNLRHLD S +THM PEG+ +LSCLQT+S FV DC ++++L ELN L +L G+L+I
Sbjct: 661 VNLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFVFDCTKTNKLRELNELNNLTGELKIT 720
Query: 721 GLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVES 780
GL+ LR P EVSLVNLK K L++L+LEWK+ ++E E DE +M+GLEP+PNVES
Sbjct: 721 GLQQLRSTPYEVSLVNLKGKKCLRSLKLEWKMGL--YKHEDEADE-RVMKGLEPNPNVES 780
Query: 781 LRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFID 840
L I GY+GVGL W+ + KLT+I + CHRLQHL Q HLQ L L L D+RSLKFID
Sbjct: 781 LGIKGYNGVGLPKWMFNCHLKLTEIEMVSCHRLQHLPQFHHLQTLKTLCLRDLRSLKFID 840
Query: 841 K--NEPSSSSSFPSLELLIIANMPNLEGWWEL-----GETQKN--WLPPTFPKLISLHIS 900
K N SSS FPSL+ L +ANM NLEGWWEL GET +N WLPPTFP+L L I
Sbjct: 841 KFDNPFSSSMFFPSLKSLCLANMANLEGWWELSRAVAGETSENIQWLPPTFPELEILDIY 900
Query: 901 GCPKLSLMPK-PASTGAVVFLCDVGVQLV-SALGP-LWGLKSLTLEEIEDLKYLEIMQNL 960
CPKL+ MPK P+ST A V LCDVGVQLV S +GP L L++L LEEI+DL L QN+
Sbjct: 901 QCPKLNSMPKLPSSTKAYVSLCDVGVQLVISTIGPVLLCLRTLRLEEIKDLNCLPFQQNI 960
Query: 961 G---------SLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEK 1020
+ PI L++L+I KC +L++LPEWI I TSLE L I +CPKLKSLP+GM +
Sbjct: 961 NHSLVISSTTTSPIPLKYLQIDKCHDLVTLPEWIYIFTSLETLSISKCPKLKSLPKGMRR 1020
Query: 1021 LESLRSFCIGGCPEVEERCKQGGEDWPKIAHIPDFEHF 1030
LESL+ CI CP +EERCK+GGEDWP I+H+P+ F
Sbjct: 1021 LESLKRLCIEDCPRLEERCKEGGEDWPNISHVPNIVFF 1053
BLAST of Lsi02G025580 vs. ExPASy TrEMBL
Match:
A0A6J1JVS7 (putative disease resistance protein RGA3 OS=Cucurbita maxima OX=3661 GN=LOC111488319 PE=4 SV=1)
HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 623/1105 (56.38%), Postives = 769/1105 (69.59%), Query Frame = 0
Query: 7 FDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSHLLENWL 66
+D+ L KLSS A Q+LGML+G+KD+L +L +VS IK V+LDAE QTKSH L++WL
Sbjct: 226 YDMLRISLKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAE-EQTKSHFLKDWL 285
Query: 67 QKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMARQIKKIW 126
+KL+EAL+DAEDVLDE+S EA+ R + AKQVRIFFS+ N +A +Y+M QIK +
Sbjct: 286 EKLKEALYDAEDVLDEISCEAVR-----RGRKAKQVRIFFSRYNPIAFDYKMTCQIKNVR 345
Query: 127 ERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKKKVKDLL 186
ERL+ I AE+K FHL E KT++GSFDQI G E+ SFSN EEVIGRDD K+ +KDLL
Sbjct: 346 ERLEVIAAERKNFHLTEIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKRVIKDLL 405
Query: 187 LDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFEIKAVVE 246
D MNV N+SF+AIVGM GIGKTTLA+SL+ND ++S+ F+LKIWVWVS FE+K VVE
Sbjct: 406 QD--MNVKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFEVKIVVE 465
Query: 247 KIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLLMSGAKG 306
KIIESAT S P+V GME+LQT+LQ+++ GKKYLLVMDD+WNESE+KW LK LLM GA+G
Sbjct: 466 KIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLLMGGARG 525
Query: 307 SKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLIHLGKEI 366
SKI+ITKRDS +A EI +MTSL TL+GL + SWSLF KVAF +G E ENP LI LGKEI
Sbjct: 526 SKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLIQLGKEI 585
Query: 367 LVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYNHLPPNL 426
LVK GGVPLVIRHIGRLLY + SEEEW+SFKNN+LLE+ Q+D+DM S+LKLSYNHL NL
Sbjct: 586 LVKCGGVPLVIRHIGRLLYIQKSEEEWISFKNNQLLEVIQRDHDMASILKLSYNHLSSNL 645
Query: 427 KQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWRFFLENS 486
KQCFAY SL K + + ++LIRQW+A GF++S +G K MEDT NDYF ELCWRFF EN
Sbjct: 646 KQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLSGCKSMEDTANDYFMELCWRFFYENF 705
Query: 487 INECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKSWSDVLS 546
+E NF++DV++HDVM DLA VAG +YV N N D+ E TRH+ FD +I W V S
Sbjct: 706 NDEHNFEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVAESTRHILFDREIGIWEQVPS 765
Query: 547 KLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTVPNSIRR 606
KL AKGLRTF L SN ++KN++NE +LDE+F F RLRVL L SN + VP+SI+R
Sbjct: 766 KLLKAKGLRTFIL--SNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRVPDSIKR 825
Query: 607 LRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRHLDFNTST 666
L HLRYLDLS N M+SLPNS+T L NLQTLNL C LK+LPR TRNLVNLRHL+ + ST
Sbjct: 826 LTHLRYLDLSGNYMKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRHLNISDST 885
Query: 667 -LTHMPEGIGRLSCLQTLSYFVLDC---KRSSQLSELNVLIHLNGDLRIIGLEYLRHNPS 726
LTHMPEG+G+L+CLQTLS FVLD + S+L ELN L L G L IIGLE L+ PS
Sbjct: 886 HLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLENLKFTPS 945
Query: 727 EVSLVNLKDIKGLKNLQLEWK-LSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDGYSGV 786
E SL NLK+ KGL++L+L WK LS D+ ++ +++ I+ GLEP+P +ES RI GY GV
Sbjct: 946 EASLANLKEKKGLRHLKLIWKNLSGDEHRFQADEE---ILAGLEPNPAIESFRISGYFGV 1005
Query: 787 GLTNW--VSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDKNEP-SS 846
L NW +SS L KLT+I+I C RLQ+L Q+ HL AL LYL ++ SLKFID +P SS
Sbjct: 1006 KLPNWLSLSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKFIDNTDPYSS 1065
Query: 847 SSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMP-KPAST 906
+ FPSLE L + +M NLE WWE + PTFP L L IS CPKL+ MP P T
Sbjct: 1066 ALFFPSLESLCLMDMLNLEAWWERESMVEEESLPTFPCLTILSISNCPKLATMPGHPPLT 1125
Query: 907 GAVVFLCDVGVQLVSA------LGPLWGLKSLTLEEIEDLKYL--EIMQNLG-------- 966
+ L VG+Q++S+ P LKSL L+ IEDLK L ++ QNL
Sbjct: 1126 D--ITLDGVGLQMMSSRRSSSNTSPSSSLKSLELKGIEDLKNLSHKLRQNLNSLKFLYIT 1185
Query: 967 --------------------------------------------------SLPIQLRH-- 1026
SLPI+++H
Sbjct: 1186 ECENLVNSLFDDTRNSSTSTRNYTELGDAMQWKFLLNLKFLRLENLPKLVSLPIEVQHWT 1245
Query: 1027 ----LKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSFCIGGCPE 1031
L I KC N SLPEWIG + SL++LEI+ECPKLKSLPEG+ +L++L I GC E
Sbjct: 1246 ALKFLIISKCPNFSSLPEWIGGMVSLQELEILECPKLKSLPEGIRELKALNHLRIAGCRE 1305
BLAST of Lsi02G025580 vs. NCBI nr
Match:
XP_038897566.1 (putative disease resistance protein RGA4 [Benincasa hispida])
HSP 1 Score: 1854.7 bits (4803), Expect = 0.0e+00
Identity = 932/1037 (89.87%), Postives = 977/1037 (94.21%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
MAEAILFD+AG ILMKLSSQAFQRLGMLFGLKD+LNKLT TVSTIKDVLLDAEGRQTK H
Sbjct: 1 MAEAILFDIAGEILMKLSSQAFQRLGMLFGLKDNLNKLTATVSTIKDVLLDAEGRQTKGH 60
Query: 61 LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
LLENWLQKLEEAL+DAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQ+A NY MAR
Sbjct: 61 LLENWLQKLEEALYDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQIAFNYSMAR 120
Query: 121 QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
QIKKIWERLDAIDAEKKQFHLHENCESK RYGSFDQIMTGRESWSFSN EEVIGRDDDKK
Sbjct: 121 QIKKIWERLDAIDAEKKQFHLHENCESKARYGSFDQIMTGRESWSFSNGEEVIGRDDDKK 180
Query: 181 KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
K+KDLLLDVNMNVTHNVS VAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIW+WVSDQFE
Sbjct: 181 KIKDLLLDVNMNVTHNVSIVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWIWVSDQFE 240
Query: 241 IKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
IKAVV+KIIESATKSNPHV GMEALQ ELQ+VIGGKKYLLVMDDVWNESEEKWHGLKPLL
Sbjct: 241 IKAVVKKIIESATKSNPHVMGMEALQAELQKVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
Query: 301 MSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLI 360
MSGAKGSKILITKRD KVAAEIESMTSLF+LEGLP+ KSWSLFSKVAFKEGKE+ENPNLI
Sbjct: 301 MSGAKGSKILITKRDRKVAAEIESMTSLFSLEGLPESKSWSLFSKVAFKEGKELENPNLI 360
Query: 361 HLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYN 420
GKEIL+K GGVPLVIRHIGRLLYS+T+EEEWMSFK+NELLEI QQDNDMTSVLKLSYN
Sbjct: 361 QTGKEILMKCGGVPLVIRHIGRLLYSRTTEEEWMSFKDNELLEIIQQDNDMTSVLKLSYN 420
Query: 421 HLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWR 480
HLPPNLKQCFAYLSLFPK KSLKINDLIRQWMALGFIE+SNG+K ME+TG YFKELCWR
Sbjct: 421 HLPPNLKQCFAYLSLFPKRKSLKINDLIRQWMALGFIETSNGSKSMEETGKGYFKELCWR 480
Query: 481 FFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKS 540
FFLENS+NECNFDNDVH+HDVMRDLATNVAGKKYV EN+N DYAF+EKTRHVSFDCKIKS
Sbjct: 481 FFLENSMNECNFDNDVHMHDVMRDLATNVAGKKYVRENINCDYAFSEKTRHVSFDCKIKS 540
Query: 541 WSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTV 600
WSDVLSKL AKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNS V
Sbjct: 541 WSDVLSKLHKAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSPMV 600
Query: 601 PNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRHL 660
PNSIRRLRHLRYLDLSENNMESLPNSITEL+NLQTLNLMYC +LKELPRDT NLVNLRHL
Sbjct: 601 PNSIRRLRHLRYLDLSENNMESLPNSITELRNLQTLNLMYCGDLKELPRDTSNLVNLRHL 660
Query: 661 DFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYLRHN 720
DFNTS LTHMPEG+G+LSCLQTLSYFVLDCKRS+QLSEL+VLIHLNG LRIIGLE LR +
Sbjct: 661 DFNTSILTHMPEGMGKLSCLQTLSYFVLDCKRSNQLSELDVLIHLNGYLRIIGLEQLR-S 720
Query: 721 PSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDGYSG 780
SEVSLVNLKDIKGLK L+LEWKLS DDQEYEGE+DETAIMEGLEPHPN+ESL IDGYSG
Sbjct: 721 ESEVSLVNLKDIKGLKKLELEWKLSGDDQEYEGENDETAIMEGLEPHPNIESLHIDGYSG 780
Query: 781 VGLTNW-VSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDKNEPSSS 840
VGL NW VS+SLSKLT I+I+KCHRLQHL+QMGHLQALTFLYLDDMRSLKFIDKNEPS S
Sbjct: 781 VGLPNWVVSTSLSKLTNIVIHKCHRLQHLSQMGHLQALTFLYLDDMRSLKFIDKNEPSFS 840
Query: 841 SSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMPKPASTGA 900
SSFPSL+LLII NMPNLEGWWELG TQKNWLP TFPKLISLHISGCP S MPKPASTGA
Sbjct: 841 SSFPSLKLLIIENMPNLEGWWELGNTQKNWLPLTFPKLISLHISGCPMFSFMPKPASTGA 900
Query: 901 VVFLCDVGVQLVSALGPLWGLKSLTLEEIEDLKYLEIM---QNLGSLPIQLRHLKIRKCS 960
VVFLCD VQL++ LGPL L+SLTLEEI+DLKYLEIM QNL SLPI+LRHL+IRKC
Sbjct: 901 VVFLCDASVQLITILGPLCSLESLTLEEIKDLKYLEIMESQQNLDSLPIRLRHLRIRKCL 960
Query: 961 NLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSFCIGGCPEVEERCKQGGED 1020
NLMSLPEWIGILTSLE+LEIMECPKLKSLPEGM+KLESLRSF IGGCPEVEERCKQGGED
Sbjct: 961 NLMSLPEWIGILTSLEELEIMECPKLKSLPEGMQKLESLRSFLIGGCPEVEERCKQGGED 1020
Query: 1021 WPKIAHIPDFEHFLLDN 1034
W KIAHIP FE FLLDN
Sbjct: 1021 WAKIAHIPHFEQFLLDN 1036
BLAST of Lsi02G025580 vs. NCBI nr
Match:
XP_031745107.1 (putative disease resistance protein RGA4 [Cucumis sativus])
HSP 1 Score: 1401.0 bits (3625), Expect = 0.0e+00
Identity = 729/1050 (69.43%), Postives = 846/1050 (80.57%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
MAEAILF VAG ILMKLSSQAFQRLGMLFGLK DLNKLT TVSTIKDVLLDAEGRQTKSH
Sbjct: 1 MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60
Query: 61 LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRD-KNAKQVRIFFSKSNQLALNYRMA 120
LL+NWL KLEEAL+DAEDVLDELSTEAL RE+MTRD KNAKQVRIFFSKSNQ+A NYRMA
Sbjct: 61 LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMA 120
Query: 121 RQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDK 180
RQIK IWERLDAIDAEK QFHL ENCES+T+YGSFD+IM GRE+WS SN+EEVIGRDDD
Sbjct: 121 RQIKNIWERLDAIDAEKTQFHLRENCESRTQYGSFDRIMMGRETWSSSNDEEVIGRDDDI 180
Query: 181 KKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQF 240
K+VK+ LLD+NMNVTHNVSF+AI GMGGIGKTTLAKSLYNDE++S +F+LKIWVWVSDQF
Sbjct: 181 KEVKERLLDMNMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQF 240
Query: 241 EIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPL 300
E++ V EK+IESATK+NP VKGMEALQ +LQ+VIG +KYLLVMDDVWNESEEKWHGLK L
Sbjct: 241 EVQVVAEKMIESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKSL 300
Query: 301 LMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNL 360
LM GA+GSK+LITKRD KVA EI+SMTSLFTLEGL + SW LFSKVAFKEGKE +P+
Sbjct: 301 LMGGARGSKVLITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPST 360
Query: 361 IHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSY 420
IHLGKEILV+ GGVPLVIRH+GR+LYSKTS+EEWMSFK+NELLE+ QQDNDMTS+LKLSY
Sbjct: 361 IHLGKEILVRCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEVIQQDNDMTSILKLSY 420
Query: 421 NHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCW 480
NHLPPNLK+CFAY SLFPKG ++I DLIRQW+A GFIE SNG K +EDTG DYF ELCW
Sbjct: 421 NHLPPNLKRCFAYSSLFPKGYKIEIKDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCW 480
Query: 481 RFFLENSINECNFDNDVHVHDVMRDLATNVAGKK-YVCENLNRDYAFNEKTRHVSFDCKI 540
RFF NS +ECN ++ V +HDVM + VAG K YV N N DY +E+T H+SFD I
Sbjct: 481 RFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGI 540
Query: 541 KSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSR 600
+SW DVLSKL AKGLRT L YEK N+I++AILDE+FSSFPRLRVL L+ S
Sbjct: 541 QSWQDVLSKLCKAKGLRTILLLFR--PYEKMNKIDKAILDELFSSFPRLRVLDLHFSQIS 600
Query: 601 TVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLR 660
VP SI++LRHLRYLDLSEN+ME +P+SI ELQNLQTLNL C ELKELPRD NLVNLR
Sbjct: 601 VVPKSIKKLRHLRYLDLSENDMELIPHSIIELQNLQTLNLTECYELKELPRDIDNLVNLR 660
Query: 661 HLDFN-TSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYL 720
HL F +T EG+ +L+CLQT+S FV DCK++++L ELN L +L G+L+IIGLE L
Sbjct: 661 HLTFEPCMEVTPTSEGMEKLTCLQTISLFVFDCKKTNKLWELNDLSYLTGELKIIGLEKL 720
Query: 721 RHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDG 780
R +PSE++L+NLKD KG + L LEWKL +D EYEGE DET IMEGLEPHPNVESL I+G
Sbjct: 721 RSSPSEITLINLKDKKGWQGLNLEWKLGKD--EYEGEADET-IMEGLEPHPNVESLSING 780
Query: 781 YSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDKNEP- 840
Y+G L NWV +SL KLT+I I C R+QHL Q LQ L L+L +RSL+FIDK++P
Sbjct: 781 YTGGALPNWVFNSLMKLTEIEIENCPRVQHLPQFNQLQDLRALHLVGLRSLEFIDKSDPY 840
Query: 841 SSSSSFPSLELLIIANMPNLEGWWELGET---------QKNWLPPTFPKLISLHISGCPK 900
SSS FPSL+ L + +MPNLEGWWELGE+ + WLPPTFP++ L I GCPK
Sbjct: 841 SSSVFFPSLKFLRLEDMPNLEGWWELGESKVVARETSGKAKWLPPTFPQVNFLRIYGCPK 900
Query: 901 LSLMPKPASTGAVVFLCDVGVQLVSALGPLWGLKSLTLEEIEDLKYL--EIMQNL----- 960
LS MPK AS GA V L D+GVQ+VS +GP+ L++ + +LKYL E Q+L
Sbjct: 901 LSSMPKLASIGADVILHDIGVQMVSTIGPVSSFMFLSMHGMTNLKYLWEEFQQDLVSSST 960
Query: 961 --GSLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSF 1020
S PI LR+L I C LMSLPEWIG+LTSLE L I ECPKLKSLPEGM++L+SL+
Sbjct: 961 STMSSPISLRYLTISGCPYLMSLPEWIGVLTSLETLHIKECPKLKSLPEGMQQLKSLKEL 1020
Query: 1021 CIGGCPEVEERCKQGGEDWPKIAHIPDFEH 1029
I CPE+E+RCKQGGEDWP I+H+P+F +
Sbjct: 1021 HIEDCPELEDRCKQGGEDWPNISHVPNFTY 1045
BLAST of Lsi02G025580 vs. NCBI nr
Match:
KAE8646395.1 (hypothetical protein Csa_016171 [Cucumis sativus])
HSP 1 Score: 1401.0 bits (3625), Expect = 0.0e+00
Identity = 729/1050 (69.43%), Postives = 846/1050 (80.57%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
MAEAILF VAG ILMKLSSQAFQRLGMLFGLK DLNKLT TVSTIKDVLLDAEGRQTKSH
Sbjct: 1 MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60
Query: 61 LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRD-KNAKQVRIFFSKSNQLALNYRMA 120
LL+NWL KLEEAL+DAEDVLDELSTEAL RE+MTRD KNAKQVRIFFSKSNQ+A NYRMA
Sbjct: 61 LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMA 120
Query: 121 RQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDK 180
RQIK IWERLDAIDAEK QFHL ENCES+T+YGSFD+IM GRE+WS SN+EEVIGRDDD
Sbjct: 121 RQIKNIWERLDAIDAEKTQFHLRENCESRTQYGSFDRIMMGRETWSSSNDEEVIGRDDDI 180
Query: 181 KKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQF 240
K+VK+ LLD+NMNVTHNVSF+AI GMGGIGKTTLAKSLYNDE++S +F+LKIWVWVSDQF
Sbjct: 181 KEVKERLLDMNMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQF 240
Query: 241 EIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPL 300
E++ V EK+IESATK+NP VKGMEALQ +LQ+VIG +KYLLVMDDVWNESEEKWHGLK L
Sbjct: 241 EVQVVAEKMIESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKSL 300
Query: 301 LMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNL 360
LM GA+GSK+LITKRD KVA EI+SMTSLFTLEGL + SW LFSKVAFKEGKE +P+
Sbjct: 301 LMGGARGSKVLITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPST 360
Query: 361 IHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSY 420
IHLGKEILV+ GGVPLVIRH+GR+LYSKTS+EEWMSFK+NELLE+ QQDNDMTS+LKLSY
Sbjct: 361 IHLGKEILVRCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEVIQQDNDMTSILKLSY 420
Query: 421 NHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCW 480
NHLPPNLK+CFAY SLFPKG ++I DLIRQW+A GFIE SNG K +EDTG DYF ELCW
Sbjct: 421 NHLPPNLKRCFAYSSLFPKGYKIEIKDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCW 480
Query: 481 RFFLENSINECNFDNDVHVHDVMRDLATNVAGKK-YVCENLNRDYAFNEKTRHVSFDCKI 540
RFF NS +ECN ++ V +HDVM + VAG K YV N N DY +E+T H+SFD I
Sbjct: 481 RFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGI 540
Query: 541 KSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSR 600
+SW DVLSKL AKGLRT L YEK N+I++AILDE+FSSFPRLRVL L+ S
Sbjct: 541 QSWQDVLSKLCKAKGLRTILLLFR--PYEKMNKIDKAILDELFSSFPRLRVLDLHFSQIS 600
Query: 601 TVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLR 660
VP SI++LRHLRYLDLSEN+ME +P+SI ELQNLQTLNL C ELKELPRD NLVNLR
Sbjct: 601 VVPKSIKKLRHLRYLDLSENDMELIPHSIIELQNLQTLNLTECYELKELPRDIDNLVNLR 660
Query: 661 HLDFN-TSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRIIGLEYL 720
HL F +T EG+ +L+CLQT+S FV DCK++++L ELN L +L G+L+IIGLE L
Sbjct: 661 HLTFEPCMEVTPTSEGMEKLTCLQTISLFVFDCKKTNKLWELNDLSYLTGELKIIGLEKL 720
Query: 721 RHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVESLRIDG 780
R +PSE++L+NLKD KG + L LEWKL +D EYEGE DET IMEGLEPHPNVESL I+G
Sbjct: 721 RSSPSEITLINLKDKKGWQGLNLEWKLGKD--EYEGEADET-IMEGLEPHPNVESLSING 780
Query: 781 YSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDKNEP- 840
Y+G L NWV +SL KLT+I I C R+QHL Q LQ L L+L +RSL+FIDK++P
Sbjct: 781 YTGGALPNWVFNSLMKLTEIEIENCPRVQHLPQFNQLQDLRALHLVGLRSLEFIDKSDPY 840
Query: 841 SSSSSFPSLELLIIANMPNLEGWWELGET---------QKNWLPPTFPKLISLHISGCPK 900
SSS FPSL+ L + +MPNLEGWWELGE+ + WLPPTFP++ L I GCPK
Sbjct: 841 SSSVFFPSLKFLRLEDMPNLEGWWELGESKVVARETSGKAKWLPPTFPQVNFLRIYGCPK 900
Query: 901 LSLMPKPASTGAVVFLCDVGVQLVSALGPLWGLKSLTLEEIEDLKYL--EIMQNL----- 960
LS MPK AS GA V L D+GVQ+VS +GP+ L++ + +LKYL E Q+L
Sbjct: 901 LSSMPKLASIGADVILHDIGVQMVSTIGPVSSFMFLSMHGMTNLKYLWEEFQQDLVSSST 960
Query: 961 --GSLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSF 1020
S PI LR+L I C LMSLPEWIG+LTSLE L I ECPKLKSLPEGM++L+SL+
Sbjct: 961 STMSSPISLRYLTISGCPYLMSLPEWIGVLTSLETLHIKECPKLKSLPEGMQQLKSLKEL 1020
Query: 1021 CIGGCPEVEERCKQGGEDWPKIAHIPDFEH 1029
I CPE+E+RCKQGGEDWP I+H+P+F +
Sbjct: 1021 HIEDCPELEDRCKQGGEDWPNISHVPNFTY 1045
BLAST of Lsi02G025580 vs. NCBI nr
Match:
XP_022147051.1 (putative disease resistance protein RGA4 [Momordica charantia])
HSP 1 Score: 1351.3 bits (3496), Expect = 0.0e+00
Identity = 720/1053 (68.38%), Postives = 836/1053 (79.39%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGR-QTKS 60
MAE +LF VAG ILMKL S A QRLGM+ G+++DL KLT TVS IK+VLLDAE QTK+
Sbjct: 1 MAEGVLFHVAGEILMKLGSIASQRLGMVRGVENDLTKLTSTVSVIKNVLLDAEEHCQTKT 60
Query: 61 -HLLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRM 120
H L++WLQKLEEAL+DAEDVLDELS+E LHREV+T+ KNAKQVRIFFS SNQ+A NYR+
Sbjct: 61 DHTLQDWLQKLEEALYDAEDVLDELSSETLHREVVTKGKNAKQVRIFFSGSNQIAFNYRV 120
Query: 121 ARQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGR-ESWSFSNEEEVIGRDD 180
AR IKKIWERLDAIDAE+KQF+L ENC+ +T YGSFDQIM GR E+ SF N EEVIGRD
Sbjct: 121 ARNIKKIWERLDAIDAERKQFNLTENCQQRTLYGSFDQIMMGRKETCSFPNVEEVIGRDY 180
Query: 181 DKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSD 240
DKKK+KDLLLD+NMNV HNVSF+ I GMGGIGKTTLAKSLY+D ++S+ F+ IW+W+SD
Sbjct: 181 DKKKLKDLLLDINMNVRHNVSFIVIAGMGGIGKTTLAKSLYHDREVSECFDSTIWIWISD 240
Query: 241 QFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLK 300
QFE++ VVEKIIESATK P+V+GMEAL +ELQEVIGGKKYLLVMDDVW E+EEKWH LK
Sbjct: 241 QFEVRVVVEKIIESATKRKPNVEGMEALNSELQEVIGGKKYLLVMDDVWKETEEKWHELK 300
Query: 301 PLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENP 360
PLLM GA GSKILITKRDSKVA EIESMTSLFTL+GL + SWSLF KVAFKE K+ N
Sbjct: 301 PLLMGGASGSKILITKRDSKVATEIESMTSLFTLKGLQESNSWSLFRKVAFKEAKDPANS 360
Query: 361 NLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQ---DNDMTSV 420
NLI LGKEIL K GGVPLVIRHIGRLLYSKTSEEEWMSFKNNELL++ QQ ++ +T +
Sbjct: 361 NLIKLGKEILAKCGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLKVIQQTDKNDGLTPI 420
Query: 421 LKLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGT-KCMEDTGNDY 480
L LSYNHLPPNLKQCFAYLSLFPKG L+I DLIRQWMA GFIESSNGT ME+ G Y
Sbjct: 421 LNLSYNHLPPNLKQCFAYLSLFPKGTRLEIKDLIRQWMAHGFIESSNGTANSMEEVGKSY 480
Query: 481 FKELCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVS 540
FKELCWRFF ENS +ECNFD+DV +HDVMR++A NVAG+KYV NLN D+ +EKTRH+
Sbjct: 481 FKELCWRFFFENSSDECNFDDDVDMHDVMREVAMNVAGEKYVHGNLNCDFVVSEKTRHIL 540
Query: 541 FDCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEIN-EAILDEVFSSFPRLRVLGL 600
FD KIK W DVL KL+ AKGLRTF LF S F ++ + E N EA+L+E+ SSFPRLRVLGL
Sbjct: 541 FDSKIKLWQDVLLKLQKAKGLRTF-LFLSYFPFKNRLEDNIEAVLEELCSSFPRLRVLGL 600
Query: 601 NNSNSRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTR 660
N+S VPNSI+ LRHLRYL+LS N MESLP SIT+LQNLQ+L+L C +L ELPRDT
Sbjct: 601 NHSKIHVVPNSIKNLRHLRYLNLSRNYMESLPTSITKLQNLQSLDLKDCYKLNELPRDTS 660
Query: 661 NLVNLRHLDF--NTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLR 720
NLVNLRHL + T HM EG+ +LSCL+TL YFVLDCKRS+ SELNV + NG L+
Sbjct: 661 NLVNLRHLVLPDHLDTPLHMLEGMRKLSCLRTLGYFVLDCKRSNMQSELNVPNYFNGRLK 720
Query: 721 IIGLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLS-RDDQEYEGEDDETAIMEGLEPHPN 780
I+GLE LR PS+V LVNLKDI+GL++L+L WKLS RDD E EG+ DE +MEGL+P N
Sbjct: 721 IVGLEQLRRTPSKVGLVNLKDIQGLRDLKLVWKLSGRDDDEGEGKGDE-RVMEGLQPQSN 780
Query: 781 VESLRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLK 840
VESLRI+GYSGVGL NWVS+SLS LTKI I CHR+QHL Q+ HLQ L ++L+DMRSLK
Sbjct: 781 VESLRIEGYSGVGLPNWVSTSLSNLTKIAICNCHRVQHLPQLAHLQTLKGIFLEDMRSLK 840
Query: 841 FIDKNEPSSSSSFPSLELLIIANMPNLEGWWE---LGETQKNWLPPTFPKLISLHISGCP 900
F+DKNEP FPSL L + NM NLEGWWE +GET P FP+L L I CP
Sbjct: 841 FVDKNEP-GLLCFPSLVSLRLKNMGNLEGWWESRVVGETL-----PIFPELQYLRIQHCP 900
Query: 901 KLSLMPKPA-STGAVVFLCDVGVQLVSALGPLWGLKSLTLEEIEDLKYLEI------MQN 960
KLSLMPK A ST FLCDVGVQ+V+A+GPLW + + L+EI+DLK LE+ Q+
Sbjct: 901 KLSLMPKIASSTLDSFFLCDVGVQVVTAIGPLWNFEVVILQEIKDLKDLEMESHQNRNQD 960
Query: 961 LGS--------LPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEK 1020
LGS P +LR L I KCSNLMSLPEWIG +TSL + I++CPKLKSL EGM +
Sbjct: 961 LGSSSLSMSSRSPTRLRFLTIEKCSNLMSLPEWIGTITSLWKVTIIDCPKLKSLLEGMRQ 1020
Query: 1021 LESLRSFCIGGCPEVEERCKQGGEDWPKIAHIP 1025
L+SL+ I GCPE+EERCK+GGEDWP IAHIP
Sbjct: 1021 LKSLKYLTIKGCPELEERCKEGGEDWPNIAHIP 1045
BLAST of Lsi02G025580 vs. NCBI nr
Match:
XP_022953917.1 (putative disease resistance protein RGA4 [Cucurbita moschata])
HSP 1 Score: 1151.0 bits (2976), Expect = 0.0e+00
Identity = 636/1111 (57.25%), Postives = 782/1111 (70.39%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSH 60
MAE +LF++A I+ KLSS A Q+LGML+G+KD+L +L +VS IK V+LDAE RQTKSH
Sbjct: 1 MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEERQTKSH 60
Query: 61 LLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMAR 120
L++WL+KL+EAL+DAEDVLDE+S EA+ R + AKQVRIFFS+ N +A +Y+M R
Sbjct: 61 FLKDWLEKLKEALYDAEDVLDEISGEAVR-----RGRKAKQVRIFFSRYNPIAFDYKMTR 120
Query: 121 QIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKK 180
QIK + ERL I AE+K FHL E KT++GSFDQI G E+ SFSN EEVIGRDD K+
Sbjct: 121 QIKNVRERLGVIAAERKNFHLTEIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKR 180
Query: 181 KVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFE 240
+KDLL D MNV N+SF+AIVGM GIGKTTLA+SL+ND ++S+ F+LKIWVWVS FE
Sbjct: 181 VIKDLLQD--MNVKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFE 240
Query: 241 IKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLL 300
+K VVEKIIESAT S P+V GME+LQT+LQ+++ GKKYLLVMDD+WNESE+KW LK LL
Sbjct: 241 VKIVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLL 300
Query: 301 MSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKEVENPNLI 360
M GA+GSKI+ITKRDS +A EI +MTSL TL+GL + SWSLF KVAF +G E ENP LI
Sbjct: 301 MGGARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLI 360
Query: 361 HLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTSVLKLSYN 420
LGKEILVK GGVPLVIRHIGRLLY + SEEEW+SFKNN+LLE+ Q+D+DM S+LKLSYN
Sbjct: 361 QLGKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEVIQRDHDMASILKLSYN 420
Query: 421 HLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDYFKELCWR 480
HLP NLKQCFAY SL K + + ++LIRQW+A GF++S NG K MEDTGNDYF ELCWR
Sbjct: 421 HLPSNLKQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLNGCKSMEDTGNDYFMELCWR 480
Query: 481 FFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKS 540
FF EN +E NF++DV++HDVM DLA VAG +YV N N D+ E TRH+ FD +I
Sbjct: 481 FFYENFNDEHNFEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVGESTRHILFDREIGI 540
Query: 541 WSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNSNSRTV 600
W V SKLR AKGLRTF L SN ++KN++NE +LDE+F F RLRVL L SN + V
Sbjct: 541 WEHVPSKLRKAKGLRTFIL--SNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRV 600
Query: 601 PNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTRNLVNLRHL 660
P+SI+RL HLRYLDLS N M+SLPNS+T L NLQTLNL C LK+LPR TRNLVNLRHL
Sbjct: 601 PDSIKRLTHLRYLDLSGNYMKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRHL 660
Query: 661 DFNTST-LTHMPEGIGRLSCLQTLSYFVLDC---KRSSQLSELNVLIHLNGDLRIIGLEY 720
+ + ST LTHMPEG+G+L+CLQTLS FVLD + S+L ELN L L G L IIGLE
Sbjct: 661 NISDSTHLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLEN 720
Query: 721 LRHNPSEVSLVNLKDIKGLKNLQLEWK-LSRDDQEYEGEDDETAIMEGLEPHPNVESLRI 780
L+ PSE SL NLK+ KGL++L L WK LS D+ ++ +++ I+ GLEP+P +ES RI
Sbjct: 721 LKFTPSEASLANLKEKKGLRHLTLIWKNLSGDEHRFQADEE---ILAGLEPNPAIESFRI 780
Query: 781 DGYSGVGLTNW--VSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFIDK 840
GY GV L NW +SS L KLT+I+I C RLQ+L Q+ HL AL LYL ++ SLKFID
Sbjct: 781 SGYFGVKLPNWLSLSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKFIDN 840
Query: 841 NEP-SSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMP 900
+P SS+ FPSLE L + +MPNLE WWE + PTFP L L IS CPKL+ MP
Sbjct: 841 TDPYSSALFFPSLESLCLMDMPNLEAWWERESMVEEEFLPTFPCLTILSISNCPKLATMP 900
Query: 901 -KPASTGAVVFLCDVGVQLVSAL------GPLWGLKSLTLEEIEDLKYL--EIMQNLG-- 960
P T + L VG+Q++S++ P LKSL L+ IEDLK L ++ QNL
Sbjct: 901 GHPPLTD--ITLDGVGLQMMSSIRSSSNTSPSSSLKSLELKGIEDLKNLSHKLRQNLNSL 960
Query: 961 --------------------------------------------------------SLPI 1020
SLPI
Sbjct: 961 KFLYITECESLVNSLFDETRSSSTSTRNYTELGDAMQWKFLHNLKFLRLENLPKLVSLPI 1020
Query: 1021 QLRH------LKIRKCSNLMSLPEWIGILTSLEDLEIMECPKLKSLPEGMEKLESLRSFC 1031
+++H L I KC N SLPEWIG + SL++LEI+ECPKLKSLPEG+ +L++L
Sbjct: 1021 EVQHWTALKFLIISKCPNFRSLPEWIGGMVSLQELEILECPKLKSLPEGIRELKALNHLR 1080
BLAST of Lsi02G025580 vs. TAIR 10
Match:
AT3G14470.1 (NB-ARC domain-containing disease resistance protein )
HSP 1 Score: 432.6 bits (1111), Expect = 9.1e-121
Identity = 313/1029 (30.42%), Postives = 518/1029 (50.34%), Query Frame = 0
Query: 1 MAEAILFDVAGAILMKLSSQAFQRLGMLFGLKDD-LNKLTGTVSTIKDVLLDAEGRQTKS 60
+ E L A+ L S+ F+ L ++ L +L+ + TI VL+DAE +Q +
Sbjct: 4 IGEMFLAAFLQALFQTLVSEPFRSFFKRRELNENLLERLSTALLTITAVLIDAEEKQITN 63
Query: 61 HLLENWLQKLEEALHDAEDVLDELSTEALHREVMTRDKNA---KQVRIFFSKSNQLALN- 120
++E W+ +L + ++ AED LD+++TEAL + ++ +Q+R S + L N
Sbjct: 64 PVVEKWVNELRDVVYHAEDALDDIATEALRLNIGAESSSSNRLRQLRGRMSLGDFLDGNS 123
Query: 121 YRMARQIKKIWERLDAIDAEKKQFHLHENCESKTRYGSFDQIMTGRESWSFSNEEEVIGR 180
+ +++K+ RL+ + +++ L E T ++ T S +E EV GR
Sbjct: 124 EHLETRLEKVTIRLERLASQRNILGLKE----LTAMIPKQRLPT----TSLVDESEVFGR 183
Query: 181 DDDKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWV 240
DDDK ++ L+ N + ++ VAIVG+GG+GKTTL++ LYND+ + YF K+W V
Sbjct: 184 DDDKDEIMRFLIPEN-GKDNGITVVAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHV 243
Query: 241 SDQFEIKAVVEKIIESATKSNPHVKGMEALQTELQEVIGGK--KYLLVMDDVWNESEEKW 300
S++F++ + +K+ ES T ++ LQ +L+E + G +LLV+DD+WNE+ W
Sbjct: 244 SEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLTGTGLPFLLVLDDLWNENFADW 303
Query: 301 HGLKPLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLEGLPDGKSWSLFSKVAFKEGKE 360
L+ + A+GS+IL+T R +VA+ I + L+ L DG WSLF K F +
Sbjct: 304 DLLRQPFIHAAQGSQILVTTRSQRVAS-IMCAVHVHNLQPLSDGDCWSLFMKTVFGNQEP 363
Query: 361 VENPNLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLEITQQDNDMTS 420
N + L + I+ K G+PL ++ +G +L + EW ++ + ++ +++
Sbjct: 364 CLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADKSNLLP 423
Query: 421 VLKLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSNGTKCMEDTGNDY 480
VL++SY +LP +LK+CFAY S+FPKG + + + ++ WMA GF++ + +K +E+ GN+Y
Sbjct: 424 VLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEY 483
Query: 481 FKELCWRFFLENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVS 540
F EL R L+ + +HD + +LA +G+ +E+TR++S
Sbjct: 484 FSELESRSLLQKT------KTRYIMHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLS 543
Query: 541 FDCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLN 600
+ + LR K LRTF L S + +++ + +++ + RLRVL L+
Sbjct: 544 YLRDNYAEPMEFEALREVKFLRTF-LPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLS 603
Query: 601 NSN-SRTVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKELPRDTR 660
+ +R P+ + + H R+LDLS +E LP S+ + NLQTL L YC LKELP D
Sbjct: 604 HYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDIS 663
Query: 661 NLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRII 720
NL+NLR+LD + L MP GRL LQTL+ F + S++SEL L L+G L+I+
Sbjct: 664 NLINLRYLDLIGTKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIV 723
Query: 721 GLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGE----DDETAIMEGLEPHP 780
L+ + + ++ + NL K L+ + W+ E +E + E L PH
Sbjct: 724 ELQRV-VDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHR 783
Query: 781 NVESLRIDGYSGVGLTNWVSS-SLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRS 840
++E L I+ Y G +W+S S S++ I + +C L +G L L L++ M
Sbjct: 784 HIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVG 843
Query: 841 LKFIDKN--------EPSSSSSFPSLELLIIANMPNLEGWWELGETQKNWLP-------- 900
L+ I + F SLE L N+P+ + W ++ T+ + P
Sbjct: 844 LQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFIL 903
Query: 901 ---------PTF-PKLISLHISGCPKLSLMP-------KPASTGAVVFLCDVGVQLVSAL 960
PTF P LISLHI C L P + T ++ CD V+ L
Sbjct: 904 RCPELTGTLPTFLPSLISLHIYKCGLLDFQPDHHEYSYRNLQTLSIKSSCDTLVKF--PL 963
Query: 961 GPLWGLKSLTLEEIEDLKYLEIMQNLGSLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDL 984
L L +++ L LE+ P LR+L+I C NL LP+ + +L+ +
Sbjct: 964 NHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLPKLNALPQNLQ-V 1011
BLAST of Lsi02G025580 vs. TAIR 10
Match:
AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )
HSP 1 Score: 364.8 bits (935), Expect = 2.3e-100
Identity = 273/897 (30.43%), Postives = 439/897 (48.94%), Query Frame = 0
Query: 35 LNKLTGTVSTIKDVLLDAEGRQTKSHLLENWLQKLEEALHDAEDVLDELSTEALHREVMT 94
L +L + T VL DA+ R +++WL +++A AED+LDEL TEAL R V+
Sbjct: 36 LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95
Query: 95 RDKNAKQVRIFFSK--SNQLALNYRMARQIKKIWERLDAIDAEKKQFHLHENCESKTRYG 154
A + F + + A+ ++ +++K+ L+ + L E S+TR
Sbjct: 96 ---EAGGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEHHVKHIEVIGLKE--YSETREP 155
Query: 155 SFDQIMTGRESWSFSNEEEVIGRDDDKKKVKDLLLDVNMNVTHNVSFVAIVGMGGIGKTT 214
+ Q R + ++GR +DK + +LLL + + +++VGM G+GKTT
Sbjct: 156 QWRQASRSRP--DDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTT 215
Query: 215 LAKSLYNDEKLSKYFELKIWVWVSDQFEIKAVVEKIIESATKSNPHVKGMEALQTELQEV 274
L + ++ND +++++FE+K+W+ F + V + +++ T S + + + +LQ +L++
Sbjct: 216 LTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKT 275
Query: 275 IGGKKYLLVMDDVWNESEEKWHGLKPLLMSGAKGSKILITKRDSKVAAEIESMTSLFTLE 334
+ GK++LLV+DD W+ES+ +W + +GSKI++T R S++ + + ++ ++
Sbjct: 276 LSGKRFLLVLDDFWSESDSEWESFQVAFTDAEEGSKIVLTTR-SEIVSTVAKAEKIYQMK 335
Query: 335 GLPDGKSWSLFSKVAFKE-GKEVENPNLIHLGKEILVKSGGVPLVIRHIGRLLYSKTSEE 394
+ + + W L S+ AF N L +GK I + G+PL R I L SK + +
Sbjct: 336 LMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPD 395
Query: 395 EWMSFKNNELLEITQQDNDMTSVLKLSYNHLPPNLKQCFAYLSLFPKGKSLKINDLIRQW 454
+W + N + N + VLKLSY+ LPP LK+CFA S+FPKG +L+ W
Sbjct: 396 DWYAVSKN----FSSYTNSILPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLW 455
Query: 455 MALGFIESSNGTKCMEDTGNDYFKEL-CWRFFLENSINECNFDNDVHVHDVMRDLATNVA 514
MA+ + ++ +ED GNDY +L FF I +F +HD+M DLA V+
Sbjct: 456 MAIDLLYQPRSSRRLEDIGNDYLGDLVAQSFFQRLDITMTSF----VMHDLMNDLAKAVS 515
Query: 515 GKKYVCENLNRDY--AFNEKTRHVSFDCKIKSWSDVLSKLRTAKGLRTFQLFSSNFEYEK 574
G C L D TRH SF S + A+ LRT F+S E
Sbjct: 516 GD--FCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLES 575
Query: 575 KNEINEAILDEVFSSFPRLRVLGLNNSNSRTVPNSIRRLRHLRYLDLSENNMESLPNSIT 634
++ E +L+ + ++ LR+L L++ +P S++ L+ LRYLDLS ++ LP +
Sbjct: 576 L-QLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSSTKIKELPEFVC 635
Query: 635 ELQNLQTLNLMYCRELKELPRDTRNLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVL 694
L NLQTL L CR+L LP+ L+NLR LD + L MP GI +L LQ LS FV+
Sbjct: 636 TLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGIKKLRSLQKLSNFVI 695
Query: 695 DCKRSSQLSELNVLIHLNGDLRIIGLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDD 754
+ L EL L HL G LRI L+ + SE LK L L L+W +
Sbjct: 696 GRLSGAGLHELKELSHLRGTLRISELQNVAF-ASEAKDAGLKRKPFLDGLILKWTVKGSG 755
Query: 755 QEYEGED----DETAIMEGLEPHPNVESLRIDGYSGVGLTNWV-SSSLSKLTKIIIYKCH 814
+ D+ ++ LEPHP++++ I+ Y G W+ SS +T + + C+
Sbjct: 756 FVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFFGITSVTLSSCN 815
Query: 815 RLQHLTQMGHLQALTFLYLDDMR-----SLKFIDKNEPSSSSSFPSLELLIIANMPNLEG 874
L +G L +L +L ++ L F S F SL++L MP +
Sbjct: 816 LCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVPFQSLQILKFYGMPRWDE 875
Query: 875 WW--ELGETQKNWLPPTFPKLISLHISGCPKL-SLMPKPASTGAVVFLCDVGVQLVS 913
W EL + FP L L I CP L P+ + V + D ++ VS
Sbjct: 876 WICPELED-------GIFPCLQKLIIQRCPSLRKKFPEGLPSSTEVTISDCPLRAVS 905
HSP 2 Score: 66.6 bits (161), Expect = 1.3e-10
Identity = 67/212 (31.60%), Postives = 98/212 (46.23%), Query Frame = 0
Query: 808 LTQMGHL----QALTFLYLDDMRSLKFIDKNEPSSSSSFPSLELLIIANMPNLEGWWELG 867
+T + HL Q L L++D L + +N + S+P+L L+I +LE +
Sbjct: 1080 VTDISHLMELPQNLQSLHIDSCDGLTSLPEN---LTESYPNLHELLIIACHSLESF---- 1139
Query: 868 ETQKNWLPPTFPKLISLHISGCPKL----SLMP-KPASTGAVVFL---CDVGVQLVSALG 927
PPT L +L+I C KL SL P + S +F+ C V +L
Sbjct: 1140 ---PGSHPPT--TLKTLYIRDCKKLNFTESLQPTRSYSQLEYLFIGSSCSNLVNFPLSLF 1199
Query: 928 PLWGLKSLTLEEIEDLKYLEIMQNLGSLPIQLRHLKIRKCSNLMSLPEWIGILTSLEDLE 987
P L+SL++ + E K I LG I L L+IR C NL + P+ L +
Sbjct: 1200 P--KLRSLSIRDCESFKTFSIHAGLGDDRIALESLEIRDCPNLETFPQGGLPTPKLSSML 1259
Query: 988 IMECPKLKSLPEGMEKLESLRSFCIGGCPEVE 1008
+ C KL++LPE + L SL S I CPE+E
Sbjct: 1260 LSNCKKLQALPEKLFGLTSLLSLFIIKCPEIE 1277
BLAST of Lsi02G025580 vs. TAIR 10
Match:
AT1G58848.1 (Disease resistance protein (CC-NBS-LRR class) family )
HSP 1 Score: 245.7 bits (626), Expect = 1.6e-64
Identity = 274/1085 (25.25%), Postives = 466/1085 (42.95%), Query Frame = 0
Query: 23 QRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSHLLENWLQKLEEALHDAEDVLDE 82
Q + G++D + +L ++ + L DA+ ++ S +++N +++++E ++D ED ++
Sbjct: 19 QECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSAVVKNCVEEIKEIIYDGEDTIET 78
Query: 83 LSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMARQIKKIWERLDAIDAEKKQFHLH 142
E + K+ +++ + AL I + R+ + + + F +
Sbjct: 79 FVLEQNLGKTSGIKKSIRRLACIIPDRRRYALG------IGGLSNRISKVIRDMQSFGVQ 138
Query: 143 EN-CESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKKKVKDLLLDVNMNVTHNVSFVA 202
+ + + D+ R +S ++ + +G + + KK+ L+D NV V+
Sbjct: 139 QAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVGYLVD-----EANVQVVS 198
Query: 203 IVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFEIKAVVEKIIESATKSNPHVKG 262
I GMGG+GKTTLAK ++N E + F+ WV VS F V +KI+ K
Sbjct: 199 ITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQKILRDLKPKEEEKKI 258
Query: 263 ME----ALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLLMSGAKGSKILITKRDSK 322
ME LQ EL ++ K L+V+DD+W +E W +KP + KG K+L+T R+
Sbjct: 259 MEMTQDTLQGELIRLLETSKSLIVLDDIW--EKEDWELIKP-IFPPTKGWKVLLTSRNES 318
Query: 323 VAAEIESMTSLFTLEGLPDGKSWSLFSKVA--FKEGKEVE-NPNLIHLGKEILVKSGGVP 382
VA + F E L SW+LF ++A K+ E + + LGK ++ GG+P
Sbjct: 319 VAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDEEKEELGKLMIKHCGGLP 378
Query: 383 LVIRHIGRLLYSKTSEEEWMSFKNNELLEIT------QQDNDMTS--VLKLSYNHLPPNL 442
L IR +G +L K + +W N + DN+ T VL LS+ LP L
Sbjct: 379 LAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNTCNYVLSLSFEELPSYL 438
Query: 443 KQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSN-GTKCMEDTGNDYFKELCWRFFL-- 502
K CF YL+ FP + + +L W A G + + + + D G+ Y +EL R +
Sbjct: 439 KHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEIIRDVGDVYIEELVRRNMVIS 498
Query: 503 ENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKSWSD 562
E + F+ H+HD+MR++ A ++ + + + V+ + +
Sbjct: 499 ERDVKTSRFET-CHLHDMMREVCLLKAKEENFLQITSSRTSTGNSLSIVTSRRLVYQYPI 558
Query: 563 VLS--------KLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNS 622
L KLR+ + +F + + +L F LRVL ++ +
Sbjct: 559 TLDVEKDINDPKLRSLVVVANTYMFWGGWSW--------MLLGSSFIRLELLRVLDIHRA 618
Query: 623 NSR--TVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKEL-PRDTR 682
+ + +SI +L HLRYL+L + +P S+ L+ L LNL+ L P +
Sbjct: 619 KLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYSLGNLKLLIYLNLVILVSGSTLVPNVLK 678
Query: 683 NLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRII 742
+ LR+L + L L+TL F ++ L +L ++ LR +
Sbjct: 679 EMQQLRYLALPKDMGRKTKLELSNLVKLETLKNF---STKNCSLEDLRGMVR----LRTL 738
Query: 743 GLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVES 802
+E + E ++ +K L++L + D E E I+ +++
Sbjct: 739 TIELRKETSLETLAASIGGLKYLESLTI------TDLGSEMRTKEAGIVFDF---VYLKT 798
Query: 803 LRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFID 862
L + Y + + S LT + + C RL+ M L+ L L ++R F
Sbjct: 799 LTLKLY--MPRLSKEQHFPSHLTTLYLQHC-RLEE-DPMPILEKLHQLKELELRRKSFSG 858
Query: 863 KNEPSSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMP 922
K SS FP L+ L I + E W++ E+ + P L +L I C KL +P
Sbjct: 859 KEMVCSSGGFPQLQKLSIKGLEEWED-WKVEES-------SMPVLHTLDIRDCRKLKQLP 918
Query: 923 K---PAS-TGAVVFLCDVGVQLVSALGPLWGLKSLTL-----------------EEIEDL 982
P+ T +F C + + L L LK L L ++ L
Sbjct: 919 DEHLPSHLTSISLFFCCLEEDPMPTLERLVHLKELQLLFRSFSGRIMVCAGSGFPQLHKL 978
Query: 983 KYLEI------MQNLGSLPIQLRHLKIRKCSNLMSLP------------------EWI-- 1030
K E+ + GS+P QL L+IR+C L LP EWI
Sbjct: 979 KLSELDGLEEWIVEDGSMP-QLHTLEIRRCPKLKKLPNGFPQLQNLELNELEEWEEWIVE 1038
BLAST of Lsi02G025580 vs. TAIR 10
Match:
AT1G59218.1 (Disease resistance protein (CC-NBS-LRR class) family )
HSP 1 Score: 245.7 bits (626), Expect = 1.6e-64
Identity = 274/1085 (25.25%), Postives = 466/1085 (42.95%), Query Frame = 0
Query: 23 QRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSHLLENWLQKLEEALHDAEDVLDE 82
Q + G++D + +L ++ + L DA+ ++ S +++N +++++E ++D ED ++
Sbjct: 19 QECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSAVVKNCVEEIKEIIYDGEDTIET 78
Query: 83 LSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMARQIKKIWERLDAIDAEKKQFHLH 142
E + K+ +++ + AL I + R+ + + + F +
Sbjct: 79 FVLEQNLGKTSGIKKSIRRLACIIPDRRRYALG------IGGLSNRISKVIRDMQSFGVQ 138
Query: 143 EN-CESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKKKVKDLLLDVNMNVTHNVSFVA 202
+ + + D+ R +S ++ + +G + + KK+ L+D NV V+
Sbjct: 139 QAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVGYLVD-----EANVQVVS 198
Query: 203 IVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFEIKAVVEKIIESATKSNPHVKG 262
I GMGG+GKTTLAK ++N E + F+ WV VS F V +KI+ K
Sbjct: 199 ITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQKILRDLKPKEEEKKI 258
Query: 263 ME----ALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLLMSGAKGSKILITKRDSK 322
ME LQ EL ++ K L+V+DD+W +E W +KP + KG K+L+T R+
Sbjct: 259 MEMTQDTLQGELIRLLETSKSLIVLDDIW--EKEDWELIKP-IFPPTKGWKVLLTSRNES 318
Query: 323 VAAEIESMTSLFTLEGLPDGKSWSLFSKVA--FKEGKEVE-NPNLIHLGKEILVKSGGVP 382
VA + F E L SW+LF ++A K+ E + + LGK ++ GG+P
Sbjct: 319 VAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDEEKEELGKLMIKHCGGLP 378
Query: 383 LVIRHIGRLLYSKTSEEEWMSFKNNELLEIT------QQDNDMTS--VLKLSYNHLPPNL 442
L IR +G +L K + +W N + DN+ T VL LS+ LP L
Sbjct: 379 LAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNTCNYVLSLSFEELPSYL 438
Query: 443 KQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSN-GTKCMEDTGNDYFKELCWRFFL-- 502
K CF YL+ FP + + +L W A G + + + + D G+ Y +EL R +
Sbjct: 439 KHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEIIRDVGDVYIEELVRRNMVIS 498
Query: 503 ENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKSWSD 562
E + F+ H+HD+MR++ A ++ + + + V+ + +
Sbjct: 499 ERDVKTSRFET-CHLHDMMREVCLLKAKEENFLQITSSRTSTGNSLSIVTSRRLVYQYPI 558
Query: 563 VLS--------KLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNS 622
L KLR+ + +F + + +L F LRVL ++ +
Sbjct: 559 TLDVEKDINDPKLRSLVVVANTYMFWGGWSW--------MLLGSSFIRLELLRVLDIHRA 618
Query: 623 NSR--TVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKEL-PRDTR 682
+ + +SI +L HLRYL+L + +P S+ L+ L LNL+ L P +
Sbjct: 619 KLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYSLGNLKLLIYLNLVILVSGSTLVPNVLK 678
Query: 683 NLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRII 742
+ LR+L + L L+TL F ++ L +L ++ LR +
Sbjct: 679 EMQQLRYLALPKDMGRKTKLELSNLVKLETLKNF---STKNCSLEDLRGMVR----LRTL 738
Query: 743 GLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVES 802
+E + E ++ +K L++L + D E E I+ +++
Sbjct: 739 TIELRKETSLETLAASIGGLKYLESLTI------TDLGSEMRTKEAGIVFDF---VYLKT 798
Query: 803 LRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFID 862
L + Y + + S LT + + C RL+ M L+ L L ++R F
Sbjct: 799 LTLKLY--MPRLSKEQHFPSHLTTLYLQHC-RLEE-DPMPILEKLHQLKELELRRKSFSG 858
Query: 863 KNEPSSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMP 922
K SS FP L+ L I + E W++ E+ + P L +L I C KL +P
Sbjct: 859 KEMVCSSGGFPQLQKLSIKGLEEWED-WKVEES-------SMPVLHTLDIRDCRKLKQLP 918
Query: 923 K---PAS-TGAVVFLCDVGVQLVSALGPLWGLKSLTL-----------------EEIEDL 982
P+ T +F C + + L L LK L L ++ L
Sbjct: 919 DEHLPSHLTSISLFFCCLEEDPMPTLERLVHLKELQLLFRSFSGRIMVCAGSGFPQLHKL 978
Query: 983 KYLEI------MQNLGSLPIQLRHLKIRKCSNLMSLP------------------EWI-- 1030
K E+ + GS+P QL L+IR+C L LP EWI
Sbjct: 979 KLSELDGLEEWIVEDGSMP-QLHTLEIRRCPKLKKLPNGFPQLQNLELNELEEWEEWIVE 1038
BLAST of Lsi02G025580 vs. TAIR 10
Match:
AT1G58848.2 (Disease resistance protein (CC-NBS-LRR class) family )
HSP 1 Score: 245.7 bits (626), Expect = 1.6e-64
Identity = 274/1085 (25.25%), Postives = 466/1085 (42.95%), Query Frame = 0
Query: 23 QRLGMLFGLKDDLNKLTGTVSTIKDVLLDAEGRQTKSHLLENWLQKLEEALHDAEDVLDE 82
Q + G++D + +L ++ + L DA+ ++ S +++N +++++E ++D ED ++
Sbjct: 19 QECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSAVVKNCVEEIKEIIYDGEDTIET 78
Query: 83 LSTEALHREVMTRDKNAKQVRIFFSKSNQLALNYRMARQIKKIWERLDAIDAEKKQFHLH 142
E + K+ +++ + AL I + R+ + + + F +
Sbjct: 79 FVLEQNLGKTSGIKKSIRRLACIIPDRRRYALG------IGGLSNRISKVIRDMQSFGVQ 138
Query: 143 EN-CESKTRYGSFDQIMTGRESWSFSNEEEVIGRDDDKKKVKDLLLDVNMNVTHNVSFVA 202
+ + + D+ R +S ++ + +G + + KK+ L+D NV V+
Sbjct: 139 QAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVGYLVD-----EANVQVVS 198
Query: 203 IVGMGGIGKTTLAKSLYNDEKLSKYFELKIWVWVSDQFEIKAVVEKIIESATKSNPHVKG 262
I GMGG+GKTTLAK ++N E + F+ WV VS F V +KI+ K
Sbjct: 199 ITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQKILRDLKPKEEEKKI 258
Query: 263 ME----ALQTELQEVIGGKKYLLVMDDVWNESEEKWHGLKPLLMSGAKGSKILITKRDSK 322
ME LQ EL ++ K L+V+DD+W +E W +KP + KG K+L+T R+
Sbjct: 259 MEMTQDTLQGELIRLLETSKSLIVLDDIW--EKEDWELIKP-IFPPTKGWKVLLTSRNES 318
Query: 323 VAAEIESMTSLFTLEGLPDGKSWSLFSKVA--FKEGKEVE-NPNLIHLGKEILVKSGGVP 382
VA + F E L SW+LF ++A K+ E + + LGK ++ GG+P
Sbjct: 319 VAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDEEKEELGKLMIKHCGGLP 378
Query: 383 LVIRHIGRLLYSKTSEEEWMSFKNNELLEIT------QQDNDMTS--VLKLSYNHLPPNL 442
L IR +G +L K + +W N + DN+ T VL LS+ LP L
Sbjct: 379 LAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNTCNYVLSLSFEELPSYL 438
Query: 443 KQCFAYLSLFPKGKSLKINDLIRQWMALGFIESSN-GTKCMEDTGNDYFKELCWRFFL-- 502
K CF YL+ FP + + +L W A G + + + + D G+ Y +EL R +
Sbjct: 439 KHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEIIRDVGDVYIEELVRRNMVIS 498
Query: 503 ENSINECNFDNDVHVHDVMRDLATNVAGKKYVCENLNRDYAFNEKTRHVSFDCKIKSWSD 562
E + F+ H+HD+MR++ A ++ + + + V+ + +
Sbjct: 499 ERDVKTSRFET-CHLHDMMREVCLLKAKEENFLQITSSRTSTGNSLSIVTSRRLVYQYPI 558
Query: 563 VLS--------KLRTAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLRVLGLNNS 622
L KLR+ + +F + + +L F LRVL ++ +
Sbjct: 559 TLDVEKDINDPKLRSLVVVANTYMFWGGWSW--------MLLGSSFIRLELLRVLDIHRA 618
Query: 623 NSR--TVPNSIRRLRHLRYLDLSENNMESLPNSITELQNLQTLNLMYCRELKEL-PRDTR 682
+ + +SI +L HLRYL+L + +P S+ L+ L LNL+ L P +
Sbjct: 619 KLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYSLGNLKLLIYLNLVILVSGSTLVPNVLK 678
Query: 683 NLVNLRHLDFNTSTLTHMPEGIGRLSCLQTLSYFVLDCKRSSQLSELNVLIHLNGDLRII 742
+ LR+L + L L+TL F ++ L +L ++ LR +
Sbjct: 679 EMQQLRYLALPKDMGRKTKLELSNLVKLETLKNF---STKNCSLEDLRGMVR----LRTL 738
Query: 743 GLEYLRHNPSEVSLVNLKDIKGLKNLQLEWKLSRDDQEYEGEDDETAIMEGLEPHPNVES 802
+E + E ++ +K L++L + D E E I+ +++
Sbjct: 739 TIELRKETSLETLAASIGGLKYLESLTI------TDLGSEMRTKEAGIVFDF---VYLKT 798
Query: 803 LRIDGYSGVGLTNWVSSSLSKLTKIIIYKCHRLQHLTQMGHLQALTFLYLDDMRSLKFID 862
L + Y + + S LT + + C RL+ M L+ L L ++R F
Sbjct: 799 LTLKLY--MPRLSKEQHFPSHLTTLYLQHC-RLEE-DPMPILEKLHQLKELELRRKSFSG 858
Query: 863 KNEPSSSSSFPSLELLIIANMPNLEGWWELGETQKNWLPPTFPKLISLHISGCPKLSLMP 922
K SS FP L+ L I + E W++ E+ + P L +L I C KL +P
Sbjct: 859 KEMVCSSGGFPQLQKLSIKGLEEWED-WKVEES-------SMPVLHTLDIRDCRKLKQLP 918
Query: 923 K---PAS-TGAVVFLCDVGVQLVSALGPLWGLKSLTL-----------------EEIEDL 982
P+ T +F C + + L L LK L L ++ L
Sbjct: 919 DEHLPSHLTSISLFFCCLEEDPMPTLERLVHLKELQLLFRSFSGRIMVCAGSGFPQLHKL 978
Query: 983 KYLEI------MQNLGSLPIQLRHLKIRKCSNLMSLP------------------EWI-- 1030
K E+ + GS+P QL L+IR+C L LP EWI
Sbjct: 979 KLSELDGLEEWIVEDGSMP-QLHTLEIRRCPKLKKLPNGFPQLQNLELNELEEWEEWIVE 1038
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q7XA39 | 2.6e-152 | 34.93 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
Q7XA40 | 1.0e-148 | 34.47 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
Q7XBQ9 | 1.3e-140 | 35.09 | Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... | [more] |
Q7XA42 | 1.0e-137 | 33.78 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
Q9LRR4 | 1.3e-119 | 30.42 | Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1D168 | 0.0e+00 | 68.38 | putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
A0A6J1GPK9 | 0.0e+00 | 57.25 | putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |
A0A1S3CEH4 | 0.0e+00 | 58.37 | disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=... | [more] |
A0A5A7UWP6 | 0.0e+00 | 58.03 | Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
A0A6J1JVS7 | 0.0e+00 | 56.38 | putative disease resistance protein RGA3 OS=Cucurbita maxima OX=3661 GN=LOC11148... | [more] |
Match Name | E-value | Identity | Description | |
AT3G14470.1 | 9.1e-121 | 30.42 | NB-ARC domain-containing disease resistance protein | [more] |
AT3G14460.1 | 2.3e-100 | 30.43 | LRR and NB-ARC domains-containing disease resistance protein | [more] |
AT1G58848.1 | 1.6e-64 | 25.25 | Disease resistance protein (CC-NBS-LRR class) family | [more] |
AT1G59218.1 | 1.6e-64 | 25.25 | Disease resistance protein (CC-NBS-LRR class) family | [more] |
AT1G58848.2 | 1.6e-64 | 25.25 | Disease resistance protein (CC-NBS-LRR class) family | [more] |