Lsi02G025370 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G025370 vs. ExPASy Swiss-Prot
Match: Q9FFZ1 (Pheophytinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPH PE=1 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 1.0e-175
Identity = 297/483 (61.49%), Postives = 375/483 (77.64%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSL--NSSFVNLSGLRKRKILCARIKARSRFSWSKHP 60
           ME++S + VP  ++V   +K A K L  N S +  SG++K +++                
Sbjct: 1   MEIISLNVVPQCSVVTWSSKLATKRLVPNRSSLLFSGVKKSRLV---------------- 60

Query: 61  RESSQPKILCSSYGGYVIDGEEGGSVSISESGESASKVQIPGLPDESNGESSAQISSCFW 120
                  I   +  GYV+ GE      I+  GES SKV IPGLPDESNGE +A+IS    
Sbjct: 61  -------IRSGNSDGYVV-GENDDLGRIARRGESTSKVLIPGLPDESNGEIAARISHSHC 120

Query: 121 EWKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMS 180
           EWKPKL VHYEKAG +N+++P VLFLPGFGVGSFHYEKQL DLGRDYRVWAIDFLGQG+S
Sbjct: 121 EWKPKLRVHYEKAGCDNLDAPAVLFLPGFGVGSFHYEKQLTDLGRDYRVWAIDFLGQGLS 180

Query: 181 LPVEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVY 240
           LP EDPT+ ++E + S+ K+  WGFGD+TEPWA +LV+S+DLW+DQV+YF+E+VIGEPVY
Sbjct: 181 LPTEDPTTMTEETSSSEDKEPFWGFGDKTEPWADQLVFSLDLWRDQVQYFVEEVIGEPVY 240

Query: 241 IVGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPD 300
           I GNSLGG+VALYFAA +P LVKGVTLLNATPFWGFFPNP+RSP+LA++FPW G FPLP+
Sbjct: 241 IAGNSLGGYVALYFAATHPHLVKGVTLLNATPFWGFFPNPVRSPKLARLFPWPGAFPLPE 300

Query: 301 SVRKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFA 360
            V+K+T+ VWQKISDPESI +IL+QVY DH+ NV+ VF RI+E TQHPAAAASFASIM A
Sbjct: 301 RVKKITELVWQKISDPESIAEILKQVYTDHSINVDKVFSRIVEVTQHPAAAASFASIMLA 360

Query: 361 PQGKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPH 420
           P G+LSF EAL+RC  N+V +CLMYG+EDPWV+P+WG ++KK +P APYYEISPAGHCPH
Sbjct: 361 PGGELSFSEALSRCKENNVQICLMYGREDPWVRPLWGKKIKKEIPNAPYYEISPAGHCPH 420

Query: 421 DEVPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGED-KVVKDLEFAREGSKKSVSVRL 480
           DEVPEVVN+L+RGWIK+LE+ G  ALPL+ + +   E+ ++ +++EF R+G KK+V++ L
Sbjct: 421 DEVPEVVNYLMRGWIKHLESGGFEALPLLEDTEEDWEESRIGREIEFPRDGWKKAVNLWL 459

BLAST of Lsi02G025370 vs. ExPASy Swiss-Prot
Match: O02218 (Abhydrolase domain-containing protein lid-1 OS=Caenorhabditis elegans OX=6239 GN=lid-1 PE=1 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 9.2e-07
Identity = 39/158 (24.68%), Postives = 69/158 (43.67%), Query Frame = 0

Query: 133 SENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLPVEDPTSHSKEGT 192
           +EN+N   ++F+PG G G   +        +++ V + D LG G S       S S+   
Sbjct: 66  AENLNGEAIVFIPGLGAGVAMFTANFNSCAKNHAVHSFDPLGFGRS-------SRSR--- 125

Query: 193 ESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIVGNSLGGFVALYF 252
                     F D+      E+V  ++ W+  +         E +YI+G++ GG++A  +
Sbjct: 126 ----------FSDDNAIAELEMVEVMEDWRKAMGI-------EKMYIIGHAFGGYLASAY 185

Query: 253 AACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPW 291
           A  NP  V  + L++    WGF      + +L K + W
Sbjct: 186 ALENPSRVAHLILVDP---WGFAEKVETTEKLIKPYAW 193

BLAST of Lsi02G025370 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 6.6e-05
Identity = 75/318 (23.58%), Postives = 125/318 (39.31%), Query Frame = 0

Query: 125 SVH-YEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLPVED 184
           +VH Y +A     + P +L + G G  S  +      L + + V A D LG G S     
Sbjct: 23  TVHGYRRAFRIAGSGPAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHGQS----- 82

Query: 185 PTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIVGNS 244
                                   +P A    YS+  + + +R  +  +  E V IVG+S
Sbjct: 83  -----------------------DKPRAD---YSVAAYANGMRDLLSVLDIERVTIVGHS 142

Query: 245 LGGFVALYFAACNPELVKGVTLLNA-------TPFWGFFPNPIRSPRLAKMFPWSGKFPL 304
           LGG VA+ FA   P+LV  + L++A          +     P+ S  +A +     + PL
Sbjct: 143 LGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGSEAMALL-----RLPL 202

Query: 305 PDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIM 364
                ++   +  K     S+G  L  V             RI++    P A+A+F   +
Sbjct: 203 VLPAVQIAGRIVGKAIGTTSLGHDLPNV------------LRILDDLPEPTASAAFGRTL 262

Query: 365 FA-PQGKLSFWEALTRCHM-NSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEI-SPA 424
            A    +      L RC++  ++PV +++G +D  V PV    +          EI   +
Sbjct: 263 RAVVDWRGQMVTMLDRCYLTEAIPVQIIWGTKDV-VLPVRHAHMAHAAMPGSQLEIFEGS 291

Query: 425 GHCP-HDEVPEVVNFLLR 431
           GH P HD+    ++ + R
Sbjct: 323 GHFPFHDDPARFIDIVER 291

BLAST of Lsi02G025370 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 6.6e-05
Identity = 75/318 (23.58%), Postives = 125/318 (39.31%), Query Frame = 0

Query: 125 SVH-YEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLPVED 184
           +VH Y +A     + P +L + G G  S  +      L + + V A D LG G S     
Sbjct: 23  TVHGYRRAFRIAGSGPAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHGQS----- 82

Query: 185 PTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIVGNS 244
                                   +P A    YS+  + + +R  +  +  E V IVG+S
Sbjct: 83  -----------------------DKPRAD---YSVAAYANGMRDLLSVLDIERVTIVGHS 142

Query: 245 LGGFVALYFAACNPELVKGVTLLNA-------TPFWGFFPNPIRSPRLAKMFPWSGKFPL 304
           LGG VA+ FA   P+LV  + L++A          +     P+ S  +A +     + PL
Sbjct: 143 LGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGSEAMALL-----RLPL 202

Query: 305 PDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIM 364
                ++   +  K     S+G  L  V             RI++    P A+A+F   +
Sbjct: 203 VLPAVQIAGRIVGKAIGTTSLGHDLPNV------------LRILDDLPEPTASAAFGRTL 262

Query: 365 FA-PQGKLSFWEALTRCHM-NSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEI-SPA 424
            A    +      L RC++  ++PV +++G +D  V PV    +          EI   +
Sbjct: 263 RAVVDWRGQMVTMLDRCYLTEAIPVQIIWGTKDV-VLPVRHAHMAHAAMPGSQLEIFEGS 291

Query: 425 GHCP-HDEVPEVVNFLLR 431
           GH P HD+    ++ + R
Sbjct: 323 GHFPFHDDPARFIDIVER 291

BLAST of Lsi02G025370 vs. ExPASy Swiss-Prot
Match: P0A573 (Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2734 PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 6.6e-05
Identity = 75/318 (23.58%), Postives = 125/318 (39.31%), Query Frame = 0

Query: 125 SVH-YEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLPVED 184
           +VH Y +A     + P +L + G G  S  +      L + + V A D LG G S     
Sbjct: 23  TVHGYRRAFRIAGSGPAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHGQS----- 82

Query: 185 PTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIVGNS 244
                                   +P A    YS+  + + +R  +  +  E V IVG+S
Sbjct: 83  -----------------------DKPRAD---YSVAAYANGMRDLLSVLDIERVTIVGHS 142

Query: 245 LGGFVALYFAACNPELVKGVTLLNA-------TPFWGFFPNPIRSPRLAKMFPWSGKFPL 304
           LGG VA+ FA   P+LV  + L++A          +     P+ S  +A +     + PL
Sbjct: 143 LGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGSEAMALL-----RLPL 202

Query: 305 PDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIM 364
                ++   +  K     S+G  L  V             RI++    P A+A+F   +
Sbjct: 203 VLPAVQIAGRIVGKAIGTTSLGHDLPNV------------LRILDDLPEPTASAAFGRTL 262

Query: 365 FA-PQGKLSFWEALTRCHM-NSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEI-SPA 424
            A    +      L RC++  ++PV +++G +D  V PV    +          EI   +
Sbjct: 263 RAVVDWRGQMVTMLDRCYLTEAIPVQIIWGTKDV-VLPVRHAHMAHAAMPGSQLEIFEGS 291

Query: 425 GHCP-HDEVPEVVNFLLR 431
           GH P HD+    ++ + R
Sbjct: 323 GHFPFHDDPARFIDIVER 291

BLAST of Lsi02G025370 vs. ExPASy TrEMBL
Match: A0A0A0KBD4 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G420790 PE=4 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 4.4e-278
Identity = 466/499 (93.39%), Postives = 482/499 (96.59%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSLNSSFVNLSGLRKRKILCARIKARSRFSWSKHPRE 60
           ME LSCSS+PSSNIVFLRTKCARKSLN S VNLSG R+RKILCARIKAR+RFSWSKH  E
Sbjct: 1   MEALSCSSIPSSNIVFLRTKCARKSLNPSCVNLSGFRRRKILCARIKARTRFSWSKHHHE 60

Query: 61  SSQPKILCSSYGGYVIDGEEGGSVSISESGESASKVQIPGLPDESNGESSAQISSCFWEW 120
           SSQ K+LCSSYGGYVIDGEEGGSVSI ESG+SASKVQIPGLPDESNGESSA+ISS FWEW
Sbjct: 61  SSQLKVLCSSYGGYVIDGEEGGSVSIPESGKSASKVQIPGLPDESNGESSAEISSGFWEW 120

Query: 121 KPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP 180
           KPKLSVHYEKAGSENV SPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP
Sbjct: 121 KPKLSVHYEKAGSENVKSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP 180

Query: 181 VEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV 240
           VEDPTSHSKEG ESDGKDS+WGFGD+TEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV
Sbjct: 181 VEDPTSHSKEGNESDGKDSSWGFGDKTEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV 240

Query: 241 GNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPDSV 300
           GNSLGGFVALYFAACNP+LVKGVTLLNATPFWGFFPNPIRSPRLAK+FPW GKFPLPD+V
Sbjct: 241 GNSLGGFVALYFAACNPDLVKGVTLLNATPFWGFFPNPIRSPRLAKLFPWGGKFPLPDNV 300

Query: 301 RKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFAPQ 360
           RKLTKFVWQKISDPESIGDILRQVYADHTTNV+DVFCRI+ETTQHPAAAASFASIMFAPQ
Sbjct: 301 RKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVETTQHPAAAASFASIMFAPQ 360

Query: 361 GKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE 420
           GKLSFWEAL+RCH NSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE
Sbjct: 361 GKLSFWEALSRCHENSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE 420

Query: 421 VPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGEDKVVKDLEFAREGSKKSVSVRLYGS 480
           VPEVVNFLLRGWIKN+ETEGLV LPLVGE D+IGEDKVVKDLEF REGSKKSVSVRLYGS
Sbjct: 421 VPEVVNFLLRGWIKNIETEGLVGLPLVGEQDTIGEDKVVKDLEFLREGSKKSVSVRLYGS 480

Query: 481 PSSVLDRITSLFKPVTVKA 500
           PSSVLDRITSLFK VTVKA
Sbjct: 481 PSSVLDRITSLFKTVTVKA 499

BLAST of Lsi02G025370 vs. ExPASy TrEMBL
Match: A0A1S3CEH2 (pheophytinase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103499960 PE=4 SV=1)

HSP 1 Score: 956.4 bits (2471), Expect = 4.6e-275
Identity = 462/499 (92.59%), Postives = 477/499 (95.59%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSLNSSFVNLSGLRKRKILCARIKARSRFSWSKHPRE 60
           MEVLSCSS+PSSNIVF RTKCARKSLN S VNLSG R+RKILCARIKAR+RFSWSKH  E
Sbjct: 1   MEVLSCSSIPSSNIVFPRTKCARKSLNPSCVNLSGFRRRKILCARIKARTRFSWSKHHHE 60

Query: 61  SSQPKILCSSYGGYVIDGEEGGSVSISESGESASKVQIPGLPDESNGESSAQISSCFWEW 120
           SSQ K+LC SYGGYV+DGEEGGSVSISESGESASKVQIPGLPDESNGESSA+ISS FWEW
Sbjct: 61  SSQLKVLCRSYGGYVLDGEEGGSVSISESGESASKVQIPGLPDESNGESSAEISSGFWEW 120

Query: 121 KPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP 180
           KPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP
Sbjct: 121 KPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP 180

Query: 181 VEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV 240
           VEDPTSHS EG ESDGKDS+WGFGD+TEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV
Sbjct: 181 VEDPTSHSNEGNESDGKDSSWGFGDKTEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV 240

Query: 241 GNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPDSV 300
           GNSLGGFVALYFAACNP+LVKGVTLLNATPFWGFFPNP RSPRLAKMFPWSGKFPLPD+V
Sbjct: 241 GNSLGGFVALYFAACNPDLVKGVTLLNATPFWGFFPNPTRSPRLAKMFPWSGKFPLPDNV 300

Query: 301 RKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFAPQ 360
           RKLTK VWQKISDPESIGD+LRQVYADHTTNV+DVF RI+ETTQHPAAAASFASIMFAPQ
Sbjct: 301 RKLTKLVWQKISDPESIGDVLRQVYADHTTNVDDVFSRIVETTQHPAAAASFASIMFAPQ 360

Query: 361 GKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE 420
           GKLSFWEALTRCH NSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE
Sbjct: 361 GKLSFWEALTRCHENSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE 420

Query: 421 VPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGEDKVVKDLEFAREGSKKSVSVRLYGS 480
           VPEVVNFLLRGWI+N+ETEGLV LPLVG  DSIGED VVKDLEF REGSKKSVSVRLYGS
Sbjct: 421 VPEVVNFLLRGWIRNMETEGLVGLPLVGAQDSIGEDNVVKDLEFLREGSKKSVSVRLYGS 480

Query: 481 PSSVLDRITSLFKPVTVKA 500
           PSSVLDRITSLFK  TVKA
Sbjct: 481 PSSVLDRITSLFKTATVKA 499

BLAST of Lsi02G025370 vs. ExPASy TrEMBL
Match: A0A5D3CG88 (Pheophytinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00220 PE=4 SV=1)

HSP 1 Score: 956.4 bits (2471), Expect = 4.6e-275
Identity = 462/499 (92.59%), Postives = 477/499 (95.59%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSLNSSFVNLSGLRKRKILCARIKARSRFSWSKHPRE 60
           MEVLSCSS+PSSNIVF RTKCARKSLN S VNLSG R+RKILCARIKAR+RFSWSKH  E
Sbjct: 1   MEVLSCSSIPSSNIVFPRTKCARKSLNPSCVNLSGFRRRKILCARIKARTRFSWSKHHHE 60

Query: 61  SSQPKILCSSYGGYVIDGEEGGSVSISESGESASKVQIPGLPDESNGESSAQISSCFWEW 120
           SSQ K+LC SYGGYV+DGEEGGSVSISESGESASKVQIPGLPDESNGESSA+ISS FWEW
Sbjct: 61  SSQLKVLCRSYGGYVLDGEEGGSVSISESGESASKVQIPGLPDESNGESSAEISSGFWEW 120

Query: 121 KPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP 180
           KPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP
Sbjct: 121 KPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP 180

Query: 181 VEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV 240
           VEDPTSHS EG ESDGKDS+WGFGD+TEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV
Sbjct: 181 VEDPTSHSNEGNESDGKDSSWGFGDKTEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV 240

Query: 241 GNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPDSV 300
           GNSLGGFVALYFAACNP+LVKGVTLLNATPFWGFFPNP RSPRLAKMFPWSGKFPLPD+V
Sbjct: 241 GNSLGGFVALYFAACNPDLVKGVTLLNATPFWGFFPNPTRSPRLAKMFPWSGKFPLPDNV 300

Query: 301 RKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFAPQ 360
           RKLTK VWQKISDPESIGD+LRQVYADHTTNV+DVF RI+ETTQHPAAAASFASIMFAPQ
Sbjct: 301 RKLTKLVWQKISDPESIGDVLRQVYADHTTNVDDVFSRIVETTQHPAAAASFASIMFAPQ 360

Query: 361 GKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE 420
           GKLSFWEALTRCH NSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE
Sbjct: 361 GKLSFWEALTRCHENSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE 420

Query: 421 VPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGEDKVVKDLEFAREGSKKSVSVRLYGS 480
           VPEVVNFLLRGWI+N+ETEGLV LPLVG  DSIGED VVKDLEF REGSKKSVSVRLYGS
Sbjct: 421 VPEVVNFLLRGWIRNMETEGLVGLPLVGAQDSIGEDNVVKDLEFLREGSKKSVSVRLYGS 480

Query: 481 PSSVLDRITSLFKPVTVKA 500
           PSSVLDRITSLFK  TVKA
Sbjct: 481 PSSVLDRITSLFKTATVKA 499

BLAST of Lsi02G025370 vs. ExPASy TrEMBL
Match: A0A6J1JS13 (pheophytinase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488389 PE=4 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 2.0e-262
Identity = 447/500 (89.40%), Postives = 471/500 (94.20%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSLNSSFVNLSGLRKRKILCARIKARSRFSWSKHPRE 60
           MEVLSCSSVP+ N++FLR+ CA K+LN SFVNLSGLRKR+ILC RIKAR RF        
Sbjct: 1   MEVLSCSSVPNINVLFLRSNCAGKTLNPSFVNLSGLRKREILCVRIKARRRF-------- 60

Query: 61  SSQPKILCSSYGGYVIDGEEGGSVSISES-GESASKVQIPGLPDESNGESSAQISSCFWE 120
            SQPK+LCSSYGGYV+DG EG SVSISES GESA+KVQIPGLPDESNGESSAQISSCFWE
Sbjct: 61  -SQPKVLCSSYGGYVVDG-EGSSVSISESGGESATKVQIPGLPDESNGESSAQISSCFWE 120

Query: 121 WKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSL 180
           WKPKLSVHYEKAG+E VNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSL
Sbjct: 121 WKPKLSVHYEKAGTEYVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSL 180

Query: 181 PVEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYI 240
           PVEDPTS SKEG ES+G+DS+WGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYI
Sbjct: 181 PVEDPTSSSKEGNESNGQDSSWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYI 240

Query: 241 VGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPDS 300
           VGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAK+FPWSGKFPLPDS
Sbjct: 241 VGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKIFPWSGKFPLPDS 300

Query: 301 VRKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFAP 360
           VRKLTKFVWQKISDPESIGDILRQVYADHTTNV++VFCRI+ETTQHPAAAASFASIMFAP
Sbjct: 301 VRKLTKFVWQKISDPESIGDILRQVYADHTTNVDNVFCRIVETTQHPAAAASFASIMFAP 360

Query: 361 QGKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHD 420
           QGKLSFWEAL+RCH NSVPVCL+YGKEDPWVKPVWGL+VK+R+PEAPYYEISPAGHCPHD
Sbjct: 361 QGKLSFWEALSRCHTNSVPVCLIYGKEDPWVKPVWGLQVKRRMPEAPYYEISPAGHCPHD 420

Query: 421 EVPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGEDKVVKDLEFAREGSKKSVSVRLYG 480
           EVPEVVNFLLRGWIKNLETEG VALPLVGE DSIG+  VVKDLEFAREGSKKSVSVRLYG
Sbjct: 421 EVPEVVNFLLRGWIKNLETEGSVALPLVGEPDSIGDGNVVKDLEFAREGSKKSVSVRLYG 480

Query: 481 SPSSVLDRITSLFKPVTVKA 500
           S SSVL+RITSLFK  TVKA
Sbjct: 481 STSSVLERITSLFKTATVKA 490

BLAST of Lsi02G025370 vs. ExPASy TrEMBL
Match: A0A6J1GPC9 (pheophytinase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455918 PE=4 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 3.7e-261
Identity = 447/501 (89.22%), Postives = 470/501 (93.81%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSLNSSFVNLSGLRKRKILCARIKARSRFSWSKHPRE 60
           MEVLSCSSVP+ N++FLR+ CA K+LN SF+NLSGLRKR+ILC RIKAR RF        
Sbjct: 1   MEVLSCSSVPNINVLFLRSNCAGKTLNPSFINLSGLRKREILCGRIKARRRF-------- 60

Query: 61  SSQPKILCSSYGGYVIDGEEGGSVSISES-GESASKVQIPGLPDESNGESSAQISSCFWE 120
            SQPKILC SYGGYV+DG EG SVSISES GESA+KVQIPGLPDESNGESSAQISSCFWE
Sbjct: 61  -SQPKILCRSYGGYVVDG-EGSSVSISESGGESATKVQIPGLPDESNGESSAQISSCFWE 120

Query: 121 WKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSL 180
           WKPKLSVHYEKAG+ENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSL
Sbjct: 121 WKPKLSVHYEKAGTENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSL 180

Query: 181 PVEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYI 240
           PVEDPTS SKEG ES+G+DS+WGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYI
Sbjct: 181 PVEDPTSSSKEGNESNGQDSSWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYI 240

Query: 241 VGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPDS 300
           VGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAK+FPWSGKFPLPDS
Sbjct: 241 VGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKIFPWSGKFPLPDS 300

Query: 301 VRKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFAP 360
           VRKLTKFVWQKISDPESIGDILRQVYADHTTNV+DVFCRI+ETTQHPAAAASFASIMFAP
Sbjct: 301 VRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVETTQHPAAAASFASIMFAP 360

Query: 361 QGKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHD 420
           QGKLSFWEAL+RCH NSVPVCL+YGKEDPWVKPVWGL+VK+RVPEAPYYEISPAGHCPHD
Sbjct: 361 QGKLSFWEALSRCHTNSVPVCLIYGKEDPWVKPVWGLQVKRRVPEAPYYEISPAGHCPHD 420

Query: 421 EVPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGEDKVVKDLEFAREGSKKSVSVRLYG 480
           EVPEVVNFLLRGWIKNLETEG VALPLVGE DSIG+  VVKDLEFAREGSKKSVSVRLYG
Sbjct: 421 EVPEVVNFLLRGWIKNLETEGSVALPLVGEPDSIGDANVVKDLEFAREGSKKSVSVRLYG 480

Query: 481 SPSSVLDR-ITSLFKPVTVKA 500
           S SSV++R ITSLFK   VKA
Sbjct: 481 STSSVVERMITSLFKTARVKA 491

BLAST of Lsi02G025370 vs. NCBI nr
Match: XP_004135952.1 (pheophytinase, chloroplastic [Cucumis sativus] >XP_011659427.1 pheophytinase, chloroplastic [Cucumis sativus] >XP_011659428.1 pheophytinase, chloroplastic [Cucumis sativus] >KGN45086.1 hypothetical protein Csa_015683 [Cucumis sativus])

HSP 1 Score: 966.5 bits (2497), Expect = 9.1e-278
Identity = 466/499 (93.39%), Postives = 482/499 (96.59%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSLNSSFVNLSGLRKRKILCARIKARSRFSWSKHPRE 60
           ME LSCSS+PSSNIVFLRTKCARKSLN S VNLSG R+RKILCARIKAR+RFSWSKH  E
Sbjct: 1   MEALSCSSIPSSNIVFLRTKCARKSLNPSCVNLSGFRRRKILCARIKARTRFSWSKHHHE 60

Query: 61  SSQPKILCSSYGGYVIDGEEGGSVSISESGESASKVQIPGLPDESNGESSAQISSCFWEW 120
           SSQ K+LCSSYGGYVIDGEEGGSVSI ESG+SASKVQIPGLPDESNGESSA+ISS FWEW
Sbjct: 61  SSQLKVLCSSYGGYVIDGEEGGSVSIPESGKSASKVQIPGLPDESNGESSAEISSGFWEW 120

Query: 121 KPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP 180
           KPKLSVHYEKAGSENV SPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP
Sbjct: 121 KPKLSVHYEKAGSENVKSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP 180

Query: 181 VEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV 240
           VEDPTSHSKEG ESDGKDS+WGFGD+TEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV
Sbjct: 181 VEDPTSHSKEGNESDGKDSSWGFGDKTEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV 240

Query: 241 GNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPDSV 300
           GNSLGGFVALYFAACNP+LVKGVTLLNATPFWGFFPNPIRSPRLAK+FPW GKFPLPD+V
Sbjct: 241 GNSLGGFVALYFAACNPDLVKGVTLLNATPFWGFFPNPIRSPRLAKLFPWGGKFPLPDNV 300

Query: 301 RKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFAPQ 360
           RKLTKFVWQKISDPESIGDILRQVYADHTTNV+DVFCRI+ETTQHPAAAASFASIMFAPQ
Sbjct: 301 RKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVETTQHPAAAASFASIMFAPQ 360

Query: 361 GKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE 420
           GKLSFWEAL+RCH NSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE
Sbjct: 361 GKLSFWEALSRCHENSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE 420

Query: 421 VPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGEDKVVKDLEFAREGSKKSVSVRLYGS 480
           VPEVVNFLLRGWIKN+ETEGLV LPLVGE D+IGEDKVVKDLEF REGSKKSVSVRLYGS
Sbjct: 421 VPEVVNFLLRGWIKNIETEGLVGLPLVGEQDTIGEDKVVKDLEFLREGSKKSVSVRLYGS 480

Query: 481 PSSVLDRITSLFKPVTVKA 500
           PSSVLDRITSLFK VTVKA
Sbjct: 481 PSSVLDRITSLFKTVTVKA 499

BLAST of Lsi02G025370 vs. NCBI nr
Match: XP_008461354.1 (PREDICTED: pheophytinase, chloroplastic [Cucumis melo] >XP_008461355.1 PREDICTED: pheophytinase, chloroplastic [Cucumis melo] >KAA0058580.1 pheophytinase [Cucumis melo var. makuwa] >TYK10380.1 pheophytinase [Cucumis melo var. makuwa])

HSP 1 Score: 956.4 bits (2471), Expect = 9.4e-275
Identity = 462/499 (92.59%), Postives = 477/499 (95.59%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSLNSSFVNLSGLRKRKILCARIKARSRFSWSKHPRE 60
           MEVLSCSS+PSSNIVF RTKCARKSLN S VNLSG R+RKILCARIKAR+RFSWSKH  E
Sbjct: 1   MEVLSCSSIPSSNIVFPRTKCARKSLNPSCVNLSGFRRRKILCARIKARTRFSWSKHHHE 60

Query: 61  SSQPKILCSSYGGYVIDGEEGGSVSISESGESASKVQIPGLPDESNGESSAQISSCFWEW 120
           SSQ K+LC SYGGYV+DGEEGGSVSISESGESASKVQIPGLPDESNGESSA+ISS FWEW
Sbjct: 61  SSQLKVLCRSYGGYVLDGEEGGSVSISESGESASKVQIPGLPDESNGESSAEISSGFWEW 120

Query: 121 KPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP 180
           KPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP
Sbjct: 121 KPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP 180

Query: 181 VEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV 240
           VEDPTSHS EG ESDGKDS+WGFGD+TEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV
Sbjct: 181 VEDPTSHSNEGNESDGKDSSWGFGDKTEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV 240

Query: 241 GNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPDSV 300
           GNSLGGFVALYFAACNP+LVKGVTLLNATPFWGFFPNP RSPRLAKMFPWSGKFPLPD+V
Sbjct: 241 GNSLGGFVALYFAACNPDLVKGVTLLNATPFWGFFPNPTRSPRLAKMFPWSGKFPLPDNV 300

Query: 301 RKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFAPQ 360
           RKLTK VWQKISDPESIGD+LRQVYADHTTNV+DVF RI+ETTQHPAAAASFASIMFAPQ
Sbjct: 301 RKLTKLVWQKISDPESIGDVLRQVYADHTTNVDDVFSRIVETTQHPAAAASFASIMFAPQ 360

Query: 361 GKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE 420
           GKLSFWEALTRCH NSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE
Sbjct: 361 GKLSFWEALTRCHENSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE 420

Query: 421 VPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGEDKVVKDLEFAREGSKKSVSVRLYGS 480
           VPEVVNFLLRGWI+N+ETEGLV LPLVG  DSIGED VVKDLEF REGSKKSVSVRLYGS
Sbjct: 421 VPEVVNFLLRGWIRNMETEGLVGLPLVGAQDSIGEDNVVKDLEFLREGSKKSVSVRLYGS 480

Query: 481 PSSVLDRITSLFKPVTVKA 500
           PSSVLDRITSLFK  TVKA
Sbjct: 481 PSSVLDRITSLFKTATVKA 499

BLAST of Lsi02G025370 vs. NCBI nr
Match: XP_038898744.1 (pheophytinase, chloroplastic [Benincasa hispida] >XP_038898745.1 pheophytinase, chloroplastic [Benincasa hispida] >XP_038898746.1 pheophytinase, chloroplastic [Benincasa hispida] >XP_038898747.1 pheophytinase, chloroplastic [Benincasa hispida] >XP_038898750.1 pheophytinase, chloroplastic [Benincasa hispida])

HSP 1 Score: 940.3 bits (2429), Expect = 7.0e-270
Identity = 456/493 (92.49%), Postives = 470/493 (95.33%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSLNSSFVNLSGLRKRKILCARIKARSRFSWSKHPRE 60
           MEVLSCSSVPSSNIVFLR KCARK LN S VNLS LRKRKILCA+IKAR+RFSWSK P E
Sbjct: 1   MEVLSCSSVPSSNIVFLRRKCARKILNPSCVNLSSLRKRKILCAKIKARTRFSWSKQPLE 60

Query: 61  SSQPKILCSSYGGYVIDGEEGGSVSISESGESASKVQIPGLPDESNGESSAQISSCFWEW 120
           SSQPKILC SYGGYVIDGEEGGSVSI ESGES SKVQIPGLPDESNGESSAQISS FWEW
Sbjct: 61  SSQPKILCRSYGGYVIDGEEGGSVSIPESGESTSKVQIPGLPDESNGESSAQISSGFWEW 120

Query: 121 KPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLP 180
           KPKLSVHYEKAG EN+NSPPVLFLPGFGVGSFHY KQLKDLG DYRVWAIDFLGQGMSLP
Sbjct: 121 KPKLSVHYEKAGCENLNSPPVLFLPGFGVGSFHYAKQLKDLGCDYRVWAIDFLGQGMSLP 180

Query: 181 VEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV 240
           VEDPTSHSKEG ESDGKDS+WGFG+ETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV
Sbjct: 181 VEDPTSHSKEGNESDGKDSSWGFGEETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYIV 240

Query: 241 GNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPDSV 300
           GNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNP RSPRLAKMFPWSGKFPLP+SV
Sbjct: 241 GNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPTRSPRLAKMFPWSGKFPLPESV 300

Query: 301 RKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFAPQ 360
           RKLTKFVWQKISDPESIGDILRQVYADHTTNV+DVFCRI+ETTQHPAAAASFASIMFAPQ
Sbjct: 301 RKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVETTQHPAAAASFASIMFAPQ 360

Query: 361 GKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE 420
           GKLSFWEAL+RCHMNSVPVCL+YGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE
Sbjct: 361 GKLSFWEALSRCHMNSVPVCLIYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDE 420

Query: 421 VPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGEDKVVKDLEFAREGSKKSVSVRLYGS 480
           VPEVVNFLLRGWIKNLETEGLV LPLV E++ IGED VVKDLEF REGSKKSVSV+LYG 
Sbjct: 421 VPEVVNFLLRGWIKNLETEGLVELPLVEESNIIGEDNVVKDLEFLREGSKKSVSVQLYGP 480

Query: 481 PSSVLDRITSLFK 494
           PSSVLDRI+SL K
Sbjct: 481 PSSVLDRISSLLK 493

BLAST of Lsi02G025370 vs. NCBI nr
Match: XP_022991881.1 (pheophytinase, chloroplastic [Cucurbita maxima] >XP_022991882.1 pheophytinase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 914.4 bits (2362), Expect = 4.1e-262
Identity = 447/500 (89.40%), Postives = 471/500 (94.20%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSLNSSFVNLSGLRKRKILCARIKARSRFSWSKHPRE 60
           MEVLSCSSVP+ N++FLR+ CA K+LN SFVNLSGLRKR+ILC RIKAR RF        
Sbjct: 1   MEVLSCSSVPNINVLFLRSNCAGKTLNPSFVNLSGLRKREILCVRIKARRRF-------- 60

Query: 61  SSQPKILCSSYGGYVIDGEEGGSVSISES-GESASKVQIPGLPDESNGESSAQISSCFWE 120
            SQPK+LCSSYGGYV+DG EG SVSISES GESA+KVQIPGLPDESNGESSAQISSCFWE
Sbjct: 61  -SQPKVLCSSYGGYVVDG-EGSSVSISESGGESATKVQIPGLPDESNGESSAQISSCFWE 120

Query: 121 WKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSL 180
           WKPKLSVHYEKAG+E VNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSL
Sbjct: 121 WKPKLSVHYEKAGTEYVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSL 180

Query: 181 PVEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYI 240
           PVEDPTS SKEG ES+G+DS+WGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYI
Sbjct: 181 PVEDPTSSSKEGNESNGQDSSWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYI 240

Query: 241 VGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPDS 300
           VGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAK+FPWSGKFPLPDS
Sbjct: 241 VGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKIFPWSGKFPLPDS 300

Query: 301 VRKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFAP 360
           VRKLTKFVWQKISDPESIGDILRQVYADHTTNV++VFCRI+ETTQHPAAAASFASIMFAP
Sbjct: 301 VRKLTKFVWQKISDPESIGDILRQVYADHTTNVDNVFCRIVETTQHPAAAASFASIMFAP 360

Query: 361 QGKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHD 420
           QGKLSFWEAL+RCH NSVPVCL+YGKEDPWVKPVWGL+VK+R+PEAPYYEISPAGHCPHD
Sbjct: 361 QGKLSFWEALSRCHTNSVPVCLIYGKEDPWVKPVWGLQVKRRMPEAPYYEISPAGHCPHD 420

Query: 421 EVPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGEDKVVKDLEFAREGSKKSVSVRLYG 480
           EVPEVVNFLLRGWIKNLETEG VALPLVGE DSIG+  VVKDLEFAREGSKKSVSVRLYG
Sbjct: 421 EVPEVVNFLLRGWIKNLETEGSVALPLVGEPDSIGDGNVVKDLEFAREGSKKSVSVRLYG 480

Query: 481 SPSSVLDRITSLFKPVTVKA 500
           S SSVL+RITSLFK  TVKA
Sbjct: 481 STSSVLERITSLFKTATVKA 490

BLAST of Lsi02G025370 vs. NCBI nr
Match: KAG7014268.1 (Pheophytinase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 911.0 bits (2353), Expect = 4.5e-261
Identity = 447/501 (89.22%), Postives = 470/501 (93.81%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSLNSSFVNLSGLRKRKILCARIKARSRFSWSKHPRE 60
           MEVLSCSSVP+ N++FLRT CA K+LN SFVNLSGLRKR+ILC RIKAR+RF        
Sbjct: 1   MEVLSCSSVPNINVLFLRTNCAGKTLNPSFVNLSGLRKREILCGRIKARTRF-------- 60

Query: 61  SSQPKILCSSYGGYVIDGEEGGSVSISES-GESASKVQIPGLPDESNGESSAQISSCFWE 120
            SQPK+LC SYGGYV+DG EG SVSISES GESA+KVQIPGLPDESNGESSAQISSCFWE
Sbjct: 61  -SQPKVLCRSYGGYVVDG-EGSSVSISESGGESATKVQIPGLPDESNGESSAQISSCFWE 120

Query: 121 WKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSL 180
           WKPKLSVHYEKAG+ENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSL
Sbjct: 121 WKPKLSVHYEKAGTENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSL 180

Query: 181 PVEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYI 240
           PVEDPTS SKEG ES+ +DS+WGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYI
Sbjct: 181 PVEDPTSSSKEGNESNDQDSSWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVYI 240

Query: 241 VGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPDS 300
           VGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNP+RSPRLAKMFPWSGKFPLPDS
Sbjct: 241 VGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPVRSPRLAKMFPWSGKFPLPDS 300

Query: 301 VRKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFAP 360
           VRKLTKFVWQKISDPESIGDILRQVYADHTTNV+DVFCRI+ETTQHPAAAASFASIMFAP
Sbjct: 301 VRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVETTQHPAAAASFASIMFAP 360

Query: 361 QGKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHD 420
           QGKLSFWEAL+RCH NSVPVCL+YGKEDPWVKPVWGL+VK+RVPEAPYYEISPAGHCPHD
Sbjct: 361 QGKLSFWEALSRCHTNSVPVCLIYGKEDPWVKPVWGLQVKRRVPEAPYYEISPAGHCPHD 420

Query: 421 EVPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGEDKVVKDLEFAREGSKKSVSVRLYG 480
           EVPEVVNFLLRGWIKNLETEG VALPLVGE DSIG+  VVKDLEFAREGSKKSVSVRLYG
Sbjct: 421 EVPEVVNFLLRGWIKNLETEGSVALPLVGEPDSIGDANVVKDLEFAREGSKKSVSVRLYG 480

Query: 481 SPSSVLDR-ITSLFKPVTVKA 500
           S SSV++R ITSLFK   VKA
Sbjct: 481 STSSVVERMITSLFKTARVKA 491

BLAST of Lsi02G025370 vs. TAIR 10
Match: AT5G13800.2 (pheophytinase )

HSP 1 Score: 617.8 bits (1592), Expect = 7.4e-177
Identity = 297/483 (61.49%), Postives = 375/483 (77.64%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSL--NSSFVNLSGLRKRKILCARIKARSRFSWSKHP 60
           ME++S + VP  ++V   +K A K L  N S +  SG++K +++                
Sbjct: 1   MEIISLNVVPQCSVVTWSSKLATKRLVPNRSSLLFSGVKKSRLV---------------- 60

Query: 61  RESSQPKILCSSYGGYVIDGEEGGSVSISESGESASKVQIPGLPDESNGESSAQISSCFW 120
                  I   +  GYV+ GE      I+  GES SKV IPGLPDESNGE +A+IS    
Sbjct: 61  -------IRSGNSDGYVV-GENDDLGRIARRGESTSKVLIPGLPDESNGEIAARISHSHC 120

Query: 121 EWKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMS 180
           EWKPKL VHYEKAG +N+++P VLFLPGFGVGSFHYEKQL DLGRDYRVWAIDFLGQG+S
Sbjct: 121 EWKPKLRVHYEKAGCDNLDAPAVLFLPGFGVGSFHYEKQLTDLGRDYRVWAIDFLGQGLS 180

Query: 181 LPVEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVY 240
           LP EDPT+ ++E + S+ K+  WGFGD+TEPWA +LV+S+DLW+DQV+YF+E+VIGEPVY
Sbjct: 181 LPTEDPTTMTEETSSSEDKEPFWGFGDKTEPWADQLVFSLDLWRDQVQYFVEEVIGEPVY 240

Query: 241 IVGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPD 300
           I GNSLGG+VALYFAA +P LVKGVTLLNATPFWGFFPNP+RSP+LA++FPW G FPLP+
Sbjct: 241 IAGNSLGGYVALYFAATHPHLVKGVTLLNATPFWGFFPNPVRSPKLARLFPWPGAFPLPE 300

Query: 301 SVRKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFA 360
            V+K+T+ VWQKISDPESI +IL+QVY DH+ NV+ VF RI+E TQHPAAAASFASIM A
Sbjct: 301 RVKKITELVWQKISDPESIAEILKQVYTDHSINVDKVFSRIVEVTQHPAAAASFASIMLA 360

Query: 361 PQGKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPH 420
           P G+LSF EAL+RC  N+V +CLMYG+EDPWV+P+WG ++KK +P APYYEISPAGHCPH
Sbjct: 361 PGGELSFSEALSRCKENNVQICLMYGREDPWVRPLWGKKIKKEIPNAPYYEISPAGHCPH 420

Query: 421 DEVPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGED-KVVKDLEFAREGSKKSVSVRL 480
           DEVPEVVN+L+RGWIK+LE+ G  ALPL+ + +   E+ ++ +++EF R+G KK+V++ L
Sbjct: 421 DEVPEVVNYLMRGWIKHLESGGFEALPLLEDTEEDWEESRIGREIEFPRDGWKKAVNLWL 459

BLAST of Lsi02G025370 vs. TAIR 10
Match: AT5G13800.1 (pheophytinase )

HSP 1 Score: 617.8 bits (1592), Expect = 7.4e-177
Identity = 297/483 (61.49%), Postives = 375/483 (77.64%), Query Frame = 0

Query: 1   MEVLSCSSVPSSNIVFLRTKCARKSL--NSSFVNLSGLRKRKILCARIKARSRFSWSKHP 60
           ME++S + VP  ++V   +K A K L  N S +  SG++K +++                
Sbjct: 1   MEIISLNVVPQCSVVTWSSKLATKRLVPNRSSLLFSGVKKSRLV---------------- 60

Query: 61  RESSQPKILCSSYGGYVIDGEEGGSVSISESGESASKVQIPGLPDESNGESSAQISSCFW 120
                  I   +  GYV+ GE      I+  GES SKV IPGLPDESNGE +A+IS    
Sbjct: 61  -------IRSGNSDGYVV-GENDDLGRIARRGESTSKVLIPGLPDESNGEIAARISHSHC 120

Query: 121 EWKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMS 180
           EWKPKL VHYEKAG +N+++P VLFLPGFGVGSFHYEKQL DLGRDYRVWAIDFLGQG+S
Sbjct: 121 EWKPKLRVHYEKAGCDNLDAPAVLFLPGFGVGSFHYEKQLTDLGRDYRVWAIDFLGQGLS 180

Query: 181 LPVEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEPVY 240
           LP EDPT+ ++E + S+ K+  WGFGD+TEPWA +LV+S+DLW+DQV+YF+E+VIGEPVY
Sbjct: 181 LPTEDPTTMTEETSSSEDKEPFWGFGDKTEPWADQLVFSLDLWRDQVQYFVEEVIGEPVY 240

Query: 241 IVGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKFPLPD 300
           I GNSLGG+VALYFAA +P LVKGVTLLNATPFWGFFPNP+RSP+LA++FPW G FPLP+
Sbjct: 241 IAGNSLGGYVALYFAATHPHLVKGVTLLNATPFWGFFPNPVRSPKLARLFPWPGAFPLPE 300

Query: 301 SVRKLTKFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFA 360
            V+K+T+ VWQKISDPESI +IL+QVY DH+ NV+ VF RI+E TQHPAAAASFASIM A
Sbjct: 301 RVKKITELVWQKISDPESIAEILKQVYTDHSINVDKVFSRIVEVTQHPAAAASFASIMLA 360

Query: 361 PQGKLSFWEALTRCHMNSVPVCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPH 420
           P G+LSF EAL+RC  N+V +CLMYG+EDPWV+P+WG ++KK +P APYYEISPAGHCPH
Sbjct: 361 PGGELSFSEALSRCKENNVQICLMYGREDPWVRPLWGKKIKKEIPNAPYYEISPAGHCPH 420

Query: 421 DEVPEVVNFLLRGWIKNLETEGLVALPLVGEADSIGED-KVVKDLEFAREGSKKSVSVRL 480
           DEVPEVVN+L+RGWIK+LE+ G  ALPL+ + +   E+ ++ +++EF R+G KK+V++ L
Sbjct: 421 DEVPEVVNYLMRGWIKHLESGGFEALPLLEDTEEDWEESRIGREIEFPRDGWKKAVNLWL 459

BLAST of Lsi02G025370 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 138.7 bits (348), Expect = 1.3e-32
Identity = 106/343 (30.90%), Postives = 159/343 (46.36%), Query Frame = 0

Query: 117 FWEWKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQG 176
           FWEW+    +HY   G       P++ + GFG   FH+   + +L + Y+V+A+D LG G
Sbjct: 26  FWEWRGH-KIHYVVQG----EGSPLVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFG 85

Query: 177 MSLPVEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEP 236
            S                   D A             + Y   +W DQV  F+++V+ EP
Sbjct: 86  WS-------------------DKAL------------IEYDAMVWTDQVIDFMKEVVKEP 145

Query: 237 VYIVGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKF-- 296
             +VGNSLGGF AL  A   PE V GV LLN+    G F    R    A     + KF  
Sbjct: 146 AVVVGNSLGGFTALSVAVGLPEQVTGVALLNSA---GQFAAESRKREEADETVIT-KFIV 205

Query: 297 -PLPDSVRKLT-KFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASF 356
            PL +  +++   F++ +   P  I  +L+ VY D +TNV+D    ++E+   PA   + 
Sbjct: 206 KPLKEIFQRVVLGFLFWQAKQPSRIESVLKSVYID-STNVDDY---LVESISKPATDPNA 265

Query: 357 ASIMFAPQGKLSFWEALTRCHMNSV------PVCLMYGKEDPWVKPVWGLEVKKRVPEAP 416
             + +    +  F    +R  ++SV      P+ L++G  DPWV P    ++K     + 
Sbjct: 266 GEVYYRLMTR--FLTNQSRYTLDSVLSKMTCPLLLVWGDLDPWVGPAKAEKIKAFYSNSS 320

Query: 417 YYEISPAGHCPHDEVPEVVNFLLRGWIKNLETEGLVALPLVGE 450
              +  AGHCPHDEVPE VN  L  W+ ++      A P+V E
Sbjct: 326 LVHLQ-AGHCPHDEVPEAVNKALLDWL-SINIASKPASPIVLE 320

BLAST of Lsi02G025370 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 138.7 bits (348), Expect = 1.3e-32
Identity = 106/343 (30.90%), Postives = 159/343 (46.36%), Query Frame = 0

Query: 117 FWEWKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQG 176
           FWEW+    +HY   G       P++ + GFG   FH+   + +L + Y+V+A+D LG G
Sbjct: 83  FWEWRGH-KIHYVVQG----EGSPLVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFG 142

Query: 177 MSLPVEDPTSHSKEGTESDGKDSAWGFGDETEPWASELVYSIDLWQDQVRYFIEQVIGEP 236
            S                   D A             + Y   +W DQV  F+++V+ EP
Sbjct: 143 WS-------------------DKAL------------IEYDAMVWTDQVIDFMKEVVKEP 202

Query: 237 VYIVGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRSPRLAKMFPWSGKF-- 296
             +VGNSLGGF AL  A   PE V GV LLN+    G F    R    A     + KF  
Sbjct: 203 AVVVGNSLGGFTALSVAVGLPEQVTGVALLNSA---GQFAAESRKREEADETVIT-KFIV 262

Query: 297 -PLPDSVRKLT-KFVWQKISDPESIGDILRQVYADHTTNVNDVFCRIIETTQHPAAAASF 356
            PL +  +++   F++ +   P  I  +L+ VY D +TNV+D    ++E+   PA   + 
Sbjct: 263 KPLKEIFQRVVLGFLFWQAKQPSRIESVLKSVYID-STNVDDY---LVESISKPATDPNA 322

Query: 357 ASIMFAPQGKLSFWEALTRCHMNSV------PVCLMYGKEDPWVKPVWGLEVKKRVPEAP 416
             + +    +  F    +R  ++SV      P+ L++G  DPWV P    ++K     + 
Sbjct: 323 GEVYYRLMTR--FLTNQSRYTLDSVLSKMTCPLLLVWGDLDPWVGPAKAEKIKAFYSNSS 377

Query: 417 YYEISPAGHCPHDEVPEVVNFLLRGWIKNLETEGLVALPLVGE 450
              +  AGHCPHDEVPE VN  L  W+ ++      A P+V E
Sbjct: 383 LVHLQ-AGHCPHDEVPEAVNKALLDWL-SINIASKPASPIVLE 377

BLAST of Lsi02G025370 vs. TAIR 10
Match: AT5G19850.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 4.4e-28
Identity = 89/355 (25.07%), Postives = 152/355 (42.82%), Query Frame = 0

Query: 96  VQIPGLPDESNGESSAQISSCFWEWKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYE 155
           V    + DE++ E   Q+ +  W+WK   S+ Y+ AG+   + P ++ + GFG  S H+ 
Sbjct: 57  VSCSSVTDEASSE-ELQVRTLTWKWK-GYSIRYQCAGT---SGPALVLVHGFGANSDHWR 116

Query: 156 KQLKDLGRDYRVWAIDFLGQGMSLPVEDPTSHSKEGTESDGKDSAWGFGDETEP--WASE 215
           K    LG+ +RV++ID +G                          +G+ D+  P  +  E
Sbjct: 117 KNTPILGKTHRVYSIDLIG--------------------------YGYSDKPNPREFGGE 176

Query: 216 LVYSIDLWQDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAACNPELVKGVTLLNAT---- 275
             Y+ + W +Q+  F   V+ +  + + NS+GG V L  A   PE+ +G+ L+N +    
Sbjct: 177 PFYTFETWGEQLNDFCLDVVKDEAFFICNSIGGLVGLQAAVSKPEICRGLMLINISLRML 236

Query: 276 ----------PFWGFFPNPIRSPRLAKMFPWSGKFPLPDSVRKLTKFVWQKISDPESIGD 335
                     PF   F N +R+  + K+F                   ++ I+ PE++  
Sbjct: 237 HIKKQPFIGRPFIKSFQNLLRNTPVGKLF-------------------FKSIAKPETVKS 296

Query: 336 ILRQVYADHTTNVNDVFCRIIETTQHPAAAASFASIMFAPQGKL-SFWEALTRCHMNSVP 395
           IL Q Y D +   +++   I+     P A   F   +    G L      L +C     P
Sbjct: 297 ILCQCYHDSSQVTDELVEAILRPGLEPGAVDVFLEFICYSGGPLPEDLLPLVKC-----P 356

Query: 396 VCLMYGKEDPWVKPVWGLEVKKRVPEAPYYEISPAGHCPHDEVPEVVNFLLRGWI 434
           V + +G++DPW     G           +  +  AGHCP DE PE+VN L+  ++
Sbjct: 357 VLIAWGEKDPWEPIELGRAYSNFDAVEDFVVLPDAGHCPQDEKPEMVNPLIESFV 356