Lsi02G025000 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G025000
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pectinesterase
Location	chr02: 31480217 .. 31481179 (+)
RNA-Seq Expression	Lsi02G025000
Synteny	Lsi02G025000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAGAGCTATTCTCATTGGCTGCAATGAATGACTTCAAAATTATTCTACTTCTTCTTCTCTTTGTGTGTAACTTGTTCCTACAATCTTATTCACTGAATGCTGAGAAGGAAAGGGAGGACTACAAGAACTGGTTGTCATGGAACCTTCAAAATTACAAGAACAAAGCCGGTTCAGTAGCCCGGTCGGCTGCCAAACTCGGGCGGCCTTATAAGGGTGGCGGAGTTCTTGATGATAAATTGAAGAAGGCAGAGACGAACAAAGTGAGAATAACCGTTAGCCAGGATGGGACGGGCGATTTTAGAACGGTTGGAGAAGCTCTTAGTAGTATTCCCAAACCTAATAGTAAAAGAGTCATTTTGGTCATCAATCCAGGAGTTTACAGGTAACATTTTTGCAGCTTGTGTAAAATTTAATTGGCAAAATTTATATATATTGTCTTGTTATAAAAATATTAATTACAAATTATGAAAAATACTTCCATCTTTGTGTATGCCACCCAAAATATAGTTTGTCACATAACAAATCTTTCTTACTTCATTTTTTAAAATATTTTTTAATTTTTTTTAGATGATTAGGGTGGGATGCACAAAAATAGATACATATCTTGAAGTGCGCTCAAATATTGCTCGTTACAAAATATCTGTATAGTTTGTAAAATCTGTAAGTTATATACCGTGTACTAATGATGTAAAATAATGTGGATCGAGTCCAGTGAAAAAATCACCATTCCTAAAAGTCTACCATTCGTGACGTTTCTTGGAAACGTCAGCGATGACCCACCAACCATCACAGGAAACGCCACGGCATCGATGACCGGAGAAGACGGAAAACCATTGGGGACTTTGAAGAGTGCAACTGTGGCTGTAGATGCCAATTATTTTGTAGCTATCAATATAAAGTTTGAGGTAAAAGATTCTTTTTGCCATAAACATTCCCAAATAAAAACAAAACTCTTGATATAG

mRNA sequence

CTAGAGCTATTCTCATTGGCTGCAATGAATGACTTCAAAATTATTCTACTTCTTCTTCTCTTTGTGTGTAACTTGTTCCTACAATCTTATTCACTGAATGCTGAGAAGGAAAGGGAGGACTACAAGAACTGGTTGTCATGGAACCTTCAAAATTACAAGAACAAAGCCGGTTCAGTAGCCCGGTCGGCTGCCAAACTCGGGCGGCCTTATAAGGGTGGCGGAGTTCTTGATGATAAATTGAAGAAGGCAGAGACGAACAAAGTGAGAATAACCGTTAGCCAGGATGGGACGGGCGATTTTAGAACGGTTGGAGAAGCTCTTAGTAGTATTCCCAAACCTAATAGTAAAAGAGTCATTTTGGTCATCAATCCAGGAGTTTACAGTCTACCATTCGTGACGTTTCTTGGAAACGTCAGCGATGACCCACCAACCATCACAGGAAACGCCACGGCATCGATGACCGGAGAAGACGGAAAACCATTGGGGACTTTGAAGAGTGCAACTGTGGCTGTAGATGCCAATTATTTTGTAGCTATCAATATAAAGTTTGAGGTAAAAGATTCTTTTTGCCATAAACATTCCCAAATAAAAACAAAACTCTTGATATAG

Coding sequence (CDS)

CTAGAGCTATTCTCATTGGCTGCAATGAATGACTTCAAAATTATTCTACTTCTTCTTCTCTTTGTGTGTAACTTGTTCCTACAATCTTATTCACTGAATGCTGAGAAGGAAAGGGAGGACTACAAGAACTGGTTGTCATGGAACCTTCAAAATTACAAGAACAAAGCCGGTTCAGTAGCCCGGTCGGCTGCCAAACTCGGGCGGCCTTATAAGGGTGGCGGAGTTCTTGATGATAAATTGAAGAAGGCAGAGACGAACAAAGTGAGAATAACCGTTAGCCAGGATGGGACGGGCGATTTTAGAACGGTTGGAGAAGCTCTTAGTAGTATTCCCAAACCTAATAGTAAAAGAGTCATTTTGGTCATCAATCCAGGAGTTTACAGTCTACCATTCGTGACGTTTCTTGGAAACGTCAGCGATGACCCACCAACCATCACAGGAAACGCCACGGCATCGATGACCGGAGAAGACGGAAAACCATTGGGGACTTTGAAGAGTGCAACTGTGGCTGTAGATGCCAATTATTTTGTAGCTATCAATATAAAGTTTGAGGTAAAAGATTCTTTTTGCCATAAACATTCCCAAATAAAAACAAAACTCTTGATATAG

Protein sequence

LELFSLAAMNDFKIILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLGRPYKGGGVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVYSLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAINIKFEVKDSFCHKHSQIKTKLLI

Homology

[Close]

BLAST of Lsi02G025000 vs. ExPASy Swiss-Prot
Match: B2VPR8 (Pectinesterase 2 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.6e-12
Identity = 43/115 (37.39%), Postives = 64/115 (55.65%), Query Frame = 0


Query: 76  LDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVYS------- 135
           +D  L  AE     I +  DG+GDF+++ EA+ SIP  N+KRVIL  +PG YS       
Sbjct: 53  MDPALVTAEGQAKVIKLKSDGSGDFKSINEAIKSIPDDNTKRVILSFSPGNYSEKVKIGM 112

Query: 136 -LPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAINIK 183
              ++TF G   ++ P +    TA+         GT+ SAT+ V++NYF A+N+K
Sbjct: 113 YKHYITFYGEDPNNMPILVFGGTAA-------EYGTVDSATLIVESNYFSAVNLK 160

[Close]

BLAST of Lsi02G025000 vs. ExPASy Swiss-Prot
Match: D8VPP5 (Pectinesterase 1 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 8.6e-12
Identity = 43/115 (37.39%), Postives = 64/115 (55.65%), Query Frame = 0


Query: 76  LDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVYS------- 135
           +D  L  AE     I +  DG+GDF+++ EA+ SIP  N+KRVIL + PG YS       
Sbjct: 53  MDPALVTAEGQTKVIKLKSDGSGDFKSINEAIKSIPDDNTKRVILSLAPGNYSEKVKIGM 112

Query: 136 -LPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAINIK 183
              ++TF G   ++ P +    TA+         GT+ SAT+ V++NYF A+N+K
Sbjct: 113 YKHYITFYGEDPNNMPILVFGGTAA-------EYGTVDSATLIVESNYFSAVNLK 160

[Close]

BLAST of Lsi02G025000 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.7e-10
Identity = 60/181 (33.15%), Postives = 93/181 (51.38%), Query Frame = 0


Query: 16  LLLLLFVCNLFLQSYSLNAEKEREDYKNWLSW-NLQNYKNKAGSVARSAAKLGRPYKGGG 75
           +LLL F   L L+S          + KN++SW +L+  ++  G + RS            
Sbjct: 10  VLLLCFGVMLCLKSSCALQIGNNNELKNYISWEDLRVVED--GRIERS---FSIKENSNW 69

Query: 76  VLDDKLKKAETNKVR--ITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY----- 135
           V  +    A    VR  I V ++G GD  TV  A+  +P  NS+RV + I PG+Y     
Sbjct: 70  VTTNANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVI 129

Query: 136 ---SLPFVTFLGNVSDDPPT-ITGNATASMTGEDGKPLGTLKSATVAVDANYFVAINIKF 185
              S P+++F+GN S    T I+ +  AS  G DGK LGT ++A+V++++++F A  I F
Sbjct: 130 VPKSKPYISFIGNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITF 185

[Close]

BLAST of Lsi02G025000 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 8.1e-10
Identity = 42/102 (41.18%), Postives = 58/102 (56.86%), Query Frame = 0


Query: 90  ITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY--------SLPFVTFLGNVSDD 149
           ITVS +G   FR+V +A+ SIPK N+K + + I PG Y        + P++TF G    D
Sbjct: 61  ITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKG-AGRD 120

Query: 150 PPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAINIKF 184
              I  +  AS  G +G+ L T ++A+V V ANYF A NI F
Sbjct: 121 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISF 161

[Close]

BLAST of Lsi02G025000 vs. ExPASy Swiss-Prot
Match: Q9LY17 (Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.8e-09
Identity = 51/176 (28.98%), Postives = 83/176 (47.16%), Query Frame = 0


Query: 14  IILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLGRPYKGG 73
           ++  L++F   + L + +    + R     W   N++ Y  + G+               
Sbjct: 8   VVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKGT--------------- 67

Query: 74  GVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY------ 133
             LD  L+ AE  +  ITV+Q G  +F+T+ EA+ SIP  N  RVI+ + PGVY      
Sbjct: 68  --LDPALEAAEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTI 127

Query: 134 --SLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAINI 182
             + PF+T LG    +   +T + TA+         GT++SAT+ V A YF A ++
Sbjct: 128 DIARPFITLLGQPGAE-TVLTYHGTAAQ-------YGTVESATLIVWAEYFQAAHL 158

[Close]

BLAST of Lsi02G025000 vs. ExPASy TrEMBL
Match: A0A5A7UTQ4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00040 PE=4 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 7.9e-77
Identity = 157/185 (84.86%), Postives = 164/185 (88.65%), Query Frame = 0


Query: 9   MNDFK-IILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLG 68
           MND+K I+LLLLLFVCNLFLQSYSLN E+EREDYKNWLSWNLQNYK KAG V RS AKLG
Sbjct: 1   MNDYKFILLLLLLFVCNLFLQSYSLNIEEEREDYKNWLSWNLQNYKKKAGLVNRSTAKLG 60

Query: 69  RPYKGGGVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY 128
           R YKGGGVLDDKLKKAE NKVRI VSQDGTGDF+TVGEA+SSIPKPNSKRVILVINPGVY
Sbjct: 61  RSYKGGGVLDDKLKKAEMNKVRIIVSQDGTGDFKTVGEAISSIPKPNSKRVILVINPGVY 120

Query: 129 --------SLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAI 185
                   SLPFVTFLGN SDD PTITGN TAS+TG DGKPLGTLKSATVAVDANYFVAI
Sbjct: 121 SEKITIPKSLPFVTFLGNGSDDQPTITGNDTASVTGADGKPLGTLKSATVAVDANYFVAI 180

[Close]

BLAST of Lsi02G025000 vs. ExPASy TrEMBL
Match: A0A0A0KBA2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G414390 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.3e-76
Identity = 155/184 (84.24%), Postives = 159/184 (86.41%), Query Frame = 0


Query: 9   MNDFKIILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLGR 68
           MNDFK ILLLLLFVCNLFLQSYSLN E+ REDYKNWLSWNLQNYK KA  V RS  KLGR
Sbjct: 1   MNDFKFILLLLLFVCNLFLQSYSLNVEERREDYKNWLSWNLQNYKKKASLVDRSTVKLGR 60

Query: 69  PYKGGGVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY- 128
            Y  GGVLDDKLKKAE NKVRI VSQDGTGDFRTVGEAL+SIPKPNSKRVILVINPGVY 
Sbjct: 61  SYNSGGVLDDKLKKAEMNKVRIIVSQDGTGDFRTVGEALNSIPKPNSKRVILVINPGVYS 120

Query: 129 -------SLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAIN 185
                  SLPFVTFLGNV DD PTITGN TASMTGEDGKPLGTLKSATVAV+ANYFVAIN
Sbjct: 121 EKIIIPKSLPFVTFLGNVIDDQPTITGNDTASMTGEDGKPLGTLKSATVAVNANYFVAIN 180

[Close]

BLAST of Lsi02G025000 vs. ExPASy TrEMBL
Match: A0A1S3CF26 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103500003 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.3e-76
Identity = 157/185 (84.86%), Postives = 163/185 (88.11%), Query Frame = 0


Query: 9   MNDFK-IILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLG 68
           MN FK I+LLLLLFVCNLFLQSYSLN E+EREDYKNWLSWNLQNYK KAG V RS AKLG
Sbjct: 1   MNHFKFILLLLLLFVCNLFLQSYSLNIEEEREDYKNWLSWNLQNYKKKAGLVNRSTAKLG 60

Query: 69  RPYKGGGVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY 128
           R YKGGGVLDDKLKKAE NKVRI VSQDGTGDF+TVGEA+SSIPKPNSKRVILVINPGVY
Sbjct: 61  RSYKGGGVLDDKLKKAEMNKVRIIVSQDGTGDFKTVGEAISSIPKPNSKRVILVINPGVY 120

Query: 129 --------SLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAI 185
                   SLPFVTFLGN SDD PTITGN TAS+TG DGKPLGTLKSATVAVDANYFVAI
Sbjct: 121 SEKITIPKSLPFVTFLGNGSDDQPTITGNDTASVTGADGKPLGTLKSATVAVDANYFVAI 180

[Close]

BLAST of Lsi02G025000 vs. ExPASy TrEMBL
Match: A0A1S4E368 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103500003 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 2.5e-70
Identity = 147/177 (83.05%), Postives = 153/177 (86.44%), Query Frame = 0


Query: 9   MNDFK-IILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLG 68
           MN FK I+LLLLLFVCNLFLQSYSLN E+EREDYKNWLSWNLQNYK KAG V RS AKLG
Sbjct: 1   MNHFKFILLLLLLFVCNLFLQSYSLNIEEEREDYKNWLSWNLQNYKKKAGLVNRSTAKLG 60

Query: 69  RPYKGGGVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY 128
           R YKGGGVLDDKLKKAE NKVRI VSQDGTGDF+TVGEA+SSIPKPNSKRVILVINPGVY
Sbjct: 61  RSYKGGGVLDDKLKKAEMNKVRIIVSQDGTGDFKTVGEAISSIPKPNSKRVILVINPGVY 120

Query: 129 SLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAINIKFE 185
                      SDD PTITGN TAS+TG DGKPLGTLKSATVAVDANYFVAIN+KFE
Sbjct: 121 -----------SDDQPTITGNDTASVTGADGKPLGTLKSATVAVDANYFVAINMKFE 166

[Close]

BLAST of Lsi02G025000 vs. ExPASy TrEMBL
Match: A0A6J1GRP9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456419 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.2e-64
Identity = 134/187 (71.66%), Postives = 154/187 (82.35%), Query Frame = 0


Query: 6   LAAMNDFKIILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAK 65
           +AAMND K++LLLLL  CNLF+QSYSL+A +EREDYKNWLSWNLQNYK KA     S   
Sbjct: 1   MAAMNDLKVLLLLLLLGCNLFMQSYSLSAVEEREDYKNWLSWNLQNYKKKAVRKPGSQPN 60

Query: 66  LGRPYKGGGVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPG 125
            G  ++GGGVLDDKL+KAE+NK+RI VSQDGTGDF+T+GEAL+SIPKPNSKR++LVINPG
Sbjct: 61  SGW-FQGGGVLDDKLRKAESNKMRIVVSQDGTGDFKTIGEALNSIPKPNSKRIVLVINPG 120

Query: 126 VYS--------LPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFV 185
           VYS        LPFVTFLG+ S DPPTITGN TA + G DG PLGTLKSATVA++ NYFV
Sbjct: 121 VYSEKINIPKTLPFVTFLGSASHDPPTITGNDTAVVIGGDGIPLGTLKSATVAINGNYFV 180

[Close]

BLAST of Lsi02G025000 vs. NCBI nr
Match: XP_038897230.1 (probable pectinesterase 53 [Benincasa hispida])

HSP 1 Score: 315.8 bits (808), Expect = 2.6e-82
Identity = 166/184 (90.22%), Postives = 169/184 (91.85%), Query Frame = 0


Query: 9   MNDFKIILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLGR 68
           MND KIILLLLLFVCNLFL SYSLNAE+ REDYKNWLSWNLQNYKNKAGSVARSAAKLGR
Sbjct: 1   MNDIKIILLLLLFVCNLFLPSYSLNAEEGREDYKNWLSWNLQNYKNKAGSVARSAAKLGR 60

Query: 69  PYKGGGVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY- 128
            YKGGGVLD KLKKAE NKVRITVSQDGTGDFRTVGEAL SIPKPNSKRVILVINPGVY 
Sbjct: 61  SYKGGGVLDYKLKKAEMNKVRITVSQDGTGDFRTVGEALGSIPKPNSKRVILVINPGVYS 120

Query: 129 -------SLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAIN 185
                  SLPFVTFLGNV+DDPPTITGNATAS+TGEDGKPLGTLKSATVAVDANYFVAIN
Sbjct: 121 EKITIPKSLPFVTFLGNVNDDPPTITGNATASVTGEDGKPLGTLKSATVAVDANYFVAIN 180

[Close]

BLAST of Lsi02G025000 vs. NCBI nr
Match: KAA0058538.1 (putative pectinesterase 53 isoform X1 [Cucumis melo var. makuwa] >TYJ96894.1 putative pectinesterase 53 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 296.6 bits (758), Expect = 1.6e-76
Identity = 157/185 (84.86%), Postives = 164/185 (88.65%), Query Frame = 0


Query: 9   MNDFK-IILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLG 68
           MND+K I+LLLLLFVCNLFLQSYSLN E+EREDYKNWLSWNLQNYK KAG V RS AKLG
Sbjct: 1   MNDYKFILLLLLLFVCNLFLQSYSLNIEEEREDYKNWLSWNLQNYKKKAGLVNRSTAKLG 60

Query: 69  RPYKGGGVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY 128
           R YKGGGVLDDKLKKAE NKVRI VSQDGTGDF+TVGEA+SSIPKPNSKRVILVINPGVY
Sbjct: 61  RSYKGGGVLDDKLKKAEMNKVRIIVSQDGTGDFKTVGEAISSIPKPNSKRVILVINPGVY 120

Query: 129 --------SLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAI 185
                   SLPFVTFLGN SDD PTITGN TAS+TG DGKPLGTLKSATVAVDANYFVAI
Sbjct: 121 SEKITIPKSLPFVTFLGNGSDDQPTITGNDTASVTGADGKPLGTLKSATVAVDANYFVAI 180

[Close]

BLAST of Lsi02G025000 vs. NCBI nr
Match: XP_004136146.1 (probable pectinesterase 53 isoform X1 [Cucumis sativus] >KGN45041.1 hypothetical protein Csa_015994 [Cucumis sativus])

HSP 1 Score: 295.0 bits (754), Expect = 4.8e-76
Identity = 155/184 (84.24%), Postives = 159/184 (86.41%), Query Frame = 0


Query: 9   MNDFKIILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLGR 68
           MNDFK ILLLLLFVCNLFLQSYSLN E+ REDYKNWLSWNLQNYK KA  V RS  KLGR
Sbjct: 1   MNDFKFILLLLLFVCNLFLQSYSLNVEERREDYKNWLSWNLQNYKKKASLVDRSTVKLGR 60

Query: 69  PYKGGGVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY- 128
            Y  GGVLDDKLKKAE NKVRI VSQDGTGDFRTVGEAL+SIPKPNSKRVILVINPGVY 
Sbjct: 61  SYNSGGVLDDKLKKAEMNKVRIIVSQDGTGDFRTVGEALNSIPKPNSKRVILVINPGVYS 120

Query: 129 -------SLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAIN 185
                  SLPFVTFLGNV DD PTITGN TASMTGEDGKPLGTLKSATVAV+ANYFVAIN
Sbjct: 121 EKIIIPKSLPFVTFLGNVIDDQPTITGNDTASMTGEDGKPLGTLKSATVAVNANYFVAIN 180

[Close]

BLAST of Lsi02G025000 vs. NCBI nr
Match: XP_031744775.1 (pectinesterase QRT1 isoform X2 [Cucumis sativus])

HSP 1 Score: 295.0 bits (754), Expect = 4.8e-76
Identity = 155/184 (84.24%), Postives = 159/184 (86.41%), Query Frame = 0


Query: 9   MNDFKIILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLGR 68
           MNDFK ILLLLLFVCNLFLQSYSLN E+ REDYKNWLSWNLQNYK KA  V RS  KLGR
Sbjct: 1   MNDFKFILLLLLFVCNLFLQSYSLNVEERREDYKNWLSWNLQNYKKKASLVDRSTVKLGR 60

Query: 69  PYKGGGVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY- 128
            Y  GGVLDDKLKKAE NKVRI VSQDGTGDFRTVGEAL+SIPKPNSKRVILVINPGVY 
Sbjct: 61  SYNSGGVLDDKLKKAEMNKVRIIVSQDGTGDFRTVGEALNSIPKPNSKRVILVINPGVYS 120

Query: 129 -------SLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAIN 185
                  SLPFVTFLGNV DD PTITGN TASMTGEDGKPLGTLKSATVAV+ANYFVAIN
Sbjct: 121 EKIIIPKSLPFVTFLGNVIDDQPTITGNDTASMTGEDGKPLGTLKSATVAVNANYFVAIN 180

[Close]

BLAST of Lsi02G025000 vs. NCBI nr
Match: XP_008461405.1 (PREDICTED: probable pectinesterase 53 isoform X1 [Cucumis melo])

HSP 1 Score: 295.0 bits (754), Expect = 4.8e-76
Identity = 157/185 (84.86%), Postives = 163/185 (88.11%), Query Frame = 0


Query: 9   MNDFK-IILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLG 68
           MN FK I+LLLLLFVCNLFLQSYSLN E+EREDYKNWLSWNLQNYK KAG V RS AKLG
Sbjct: 1   MNHFKFILLLLLLFVCNLFLQSYSLNIEEEREDYKNWLSWNLQNYKKKAGLVNRSTAKLG 60

Query: 69  RPYKGGGVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY 128
           R YKGGGVLDDKLKKAE NKVRI VSQDGTGDF+TVGEA+SSIPKPNSKRVILVINPGVY
Sbjct: 61  RSYKGGGVLDDKLKKAEMNKVRIIVSQDGTGDFKTVGEAISSIPKPNSKRVILVINPGVY 120

Query: 129 --------SLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAI 185
                   SLPFVTFLGN SDD PTITGN TAS+TG DGKPLGTLKSATVAVDANYFVAI
Sbjct: 121 SEKITIPKSLPFVTFLGNGSDDQPTITGNDTASVTGADGKPLGTLKSATVAVDANYFVAI 180

[Close]

BLAST of Lsi02G025000 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 3.4e-11
Identity = 60/181 (33.15%), Postives = 93/181 (51.38%), Query Frame = 0


Query: 16  LLLLLFVCNLFLQSYSLNAEKEREDYKNWLSW-NLQNYKNKAGSVARSAAKLGRPYKGGG 75
           +LLL F   L L+S          + KN++SW +L+  ++  G + RS            
Sbjct: 10  VLLLCFGVMLCLKSSCALQIGNNNELKNYISWEDLRVVED--GRIERS---FSIKENSNW 69

Query: 76  VLDDKLKKAETNKVR--ITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY----- 135
           V  +    A    VR  I V ++G GD  TV  A+  +P  NS+RV + I PG+Y     
Sbjct: 70  VTTNANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVI 129

Query: 136 ---SLPFVTFLGNVSDDPPT-ITGNATASMTGEDGKPLGTLKSATVAVDANYFVAINIKF 185
              S P+++F+GN S    T I+ +  AS  G DGK LGT ++A+V++++++F A  I F
Sbjct: 130 VPKSKPYISFIGNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITF 185

[Close]

BLAST of Lsi02G025000 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 5.7e-11
Identity = 42/102 (41.18%), Postives = 58/102 (56.86%), Query Frame = 0


Query: 90  ITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY--------SLPFVTFLGNVSDD 149
           ITVS +G   FR+V +A+ SIPK N+K + + I PG Y        + P++TF G    D
Sbjct: 61  ITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKG-AGRD 120

Query: 150 PPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAINIKF 184
              I  +  AS  G +G+ L T ++A+V V ANYF A NI F
Sbjct: 121 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISF 161

[Close]

BLAST of Lsi02G025000 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.3e-10
Identity = 51/176 (28.98%), Postives = 83/176 (47.16%), Query Frame = 0


Query: 14  IILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLGRPYKGG 73
           ++  L++F   + L + +    + R     W   N++ Y  + G+               
Sbjct: 8   VVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKGT--------------- 67

Query: 74  GVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY------ 133
             LD  L+ AE  +  ITV+Q G  +F+T+ EA+ SIP  N  RVI+ + PGVY      
Sbjct: 68  --LDPALEAAEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTI 127

Query: 134 --SLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAINI 182
             + PF+T LG    +   +T + TA+         GT++SAT+ V A YF A ++
Sbjct: 128 DIARPFITLLGQPGAE-TVLTYHGTAAQ-------YGTVESATLIVWAEYFQAAHL 158

[Close]

BLAST of Lsi02G025000 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 4.8e-10
Identity = 51/176 (28.98%), Postives = 80/176 (45.45%), Query Frame = 0


Query: 14  IILLLLLFVCNLFLQSYSLNAEKEREDYKNWLSWNLQNYKNKAGSVARSAAKLGRPYKGG 73
           ++ LL+ F   + L         +R     W   N++ +  + G+               
Sbjct: 8   LVALLVFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRRGT--------------- 67

Query: 74  GVLDDKLKKAETNKVRITVSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY------ 133
             LD +L+ AE ++  I V+Q+G GDF+T+  A+ SIP  N  RVI+ + PG+Y      
Sbjct: 68  --LDPELEAAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTV 127

Query: 134 --SLPFVTFLGNVSDDPPTITGNATASMTGEDGKPLGTLKSATVAVDANYFVAINI 182
               P+VT LG      P    N T + T       GT++SAT+ V A  F+A N+
Sbjct: 128 DVGRPYVTLLGK-----PGAETNLTYAGT---AAKYGTVESATLIVWATNFLAANL 158

[Close]

BLAST of Lsi02G025000 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 6.3e-10
Identity = 38/98 (38.78%), Postives = 59/98 (60.20%), Query Frame = 0


Query: 92  VSQDGTGDFRTVGEALSSIPKPNSKRVILVINPGVY--------SLPFVTFLGNVSDDPP 151
           V Q+G G F+T+ EA++S+   N++RVI+ I PGVY        S PF+T  G+  +  P
Sbjct: 45  VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGH-PNAMP 104

Query: 152 TITGNATASMTGEDGKPLGTLKSATVAVDANYFVAINI 182
            +T + TA+         GT+ SAT+ V ++YF+A+NI
Sbjct: 105 VLTFDGTAAQ-------YGTVDSATLIVLSDYFMAVNI 134

The following BLAST results are available for this feature:

BLAST of Lsi02G025000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B2VPR8	6.6e-12	37.39	Pectinesterase 2 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
D8VPP5	8.6e-12	37.39	Pectinesterase 1 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q9FM79	4.7e-10	33.15	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1	[more]
Q8LPF3	8.1e-10	41.18	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]
Q9LY17	1.8e-09	28.98	Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1	[more]

BLAST of Lsi02G025000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UTQ4	7.9e-77	84.86	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00040...	[more]
A0A0A0KBA2	2.3e-76	84.24	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G414390 PE=4 SV=1	[more]
A0A1S3CF26	2.3e-76	84.86	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103500003 PE=4 SV=1	[more]
A0A1S4E368	2.5e-70	83.05	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103500003 PE=4 SV=1	[more]
A0A6J1GRP9	1.2e-64	71.66	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456419 PE=4 SV=1	[more]

BLAST of Lsi02G025000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897230.1	2.6e-82	90.22	probable pectinesterase 53 [Benincasa hispida]	[more]
KAA0058538.1	1.6e-76	84.86	putative pectinesterase 53 isoform X1 [Cucumis melo var. makuwa] >TYJ96894.1 put...	[more]
XP_004136146.1	4.8e-76	84.24	probable pectinesterase 53 isoform X1 [Cucumis sativus] >KGN45041.1 hypothetical...	[more]
XP_031744775.1	4.8e-76	84.24	pectinesterase QRT1 isoform X2 [Cucumis sativus]	[more]
XP_008461405.1	4.8e-76	84.86	PREDICTED: probable pectinesterase 53 isoform X1 [Cucumis melo]	[more]

BLAST of Lsi02G025000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55590.1	3.4e-11	33.15	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	5.7e-11	41.18	Pectin lyase-like superfamily protein	[more]
AT5G07430.1	1.3e-10	28.98	Pectin lyase-like superfamily protein	[more]
AT5G07420.1	4.8e-10	28.98	Pectin lyase-like superfamily protein	[more]
AT5G61680.1	6.3e-10	38.78	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 49..188 e-value: 2.0E-19 score: 71.9
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 90..184 e-value: 1.7E-10 score: 40.1
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 20..188
None	No IPR available	PANTHER	PTHR31321:SF81	PECTINESTERASE	coord: 20..188
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 87..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G025000.1	Lsi02G025000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity