Lsi02G024900 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G024900
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionWRKY transcription factor
Locationchr02: 31363690 .. 31366926 (+)
RNA-Seq ExpressionLsi02G024900
SyntenyLsi02G024900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGAGGTGGCGGCAGCCAATTCGTTGCGATATCAGTTTCTCGATGATGACGAGAACAAGAATCATCTAGGGTTCATGGAGCTTCTCAGTGTGGATCAAGATTTCTCATCGCAATTTGATGTCTTTGAAACATCATCGTCTTTATCGTCTTCCCTGATTTCAAATCCTGTTATGAACTCTGAGAATTTGGAGATTTGGAATCAATGGCCGACCACACCGAATTACTCGTCGTCGATCTCATCCACCTCGAGTGAGATCGTCAATGGCGAGCCGACTAAGCTAAATCAAGAGGGAGGAGAGAAACAACATCGCCAACAAACTGTAAAAACTAACAAGCAGTGAGTGTTTTATTTTCTCATCATTAAAAAAAATTATGGGCATTATTATAATGAAAATTGGACAAAATTAGACCATATTTAAGCAAGATTTTTTTTTTCTATAATCTGGGCTTTTTTCAACTTAAATAAGTTTTTTTTTCTTTTTTTATTAAAAAAATCTAAGGACCCTATTTTTTTAATATATATATGCTCTTAAGTTTTGTATGATTTTTAGACTTTTCTTTAATTAGTTATTGCCAAATTTAAAAGTTCTAATTTGTATTCAGATTTTCAATTTGATTATTATCAGTGCACTTTTTGTTAGGAGATTCACGAAATGGAATATTTAATAATCCATAGTTTCATTTATATAATGATTTGATATTTTGTGGAATAGAATTGAAATGTTGAATAATCATGAACTCTTTTAAGTCTTATTTATGTATTTATATAATCCATCTATTAACCATTTCGTTTTGATTTTTAATTTTGAAAATCATTTTTTTTTTTTTTTTTTTATAGAAACGTTAGAATTTTAGTTAAATTTTAAAACTATTTTAATTATTTATTTTCAAATAGGCTAAGTTTTGTGTTTTATTTTTGAATGCTATATATAGGTTGAAAACAAAGAAGACGAGTACAAAGAAGAAAGATCAAGAACAACGATTCGCTTTCATGACAAAGAGTGAAGTTGATCATTTGGAAGATGGATATAGATGGAGAAAGTATGGTCAAAAAGCAGTGAAAAACAGCCCTTATCCTAGGTAAAATTTTAACATAATTTTCTATTTTTACGTCTTATTTGTGAAGTTTTTTTTATTTTCATTGCAATTCAACATCTTTGATAAAAACAAATCAAAATAATAGAGTTTGGTTTTGTTGGTTATCTAATTTTCAAAATTTAAGGATCTATGTAGTTAACAATAAATCTTTTTAAAAATTATTTTTGTTAACTATATTATTCATCTTTTTTTCACACTACATTCTAAAAAGAAAAAAAGATGAAAAAAACTAAACATACATACTCAGTTACTGCCTATTTATTTAGGATTGAAAAAAAATAGTAATAACATTAAATTATTAGATAAGTCTTAGGGCTGTTTTTAAATATAGAAAAATGAGCAAACTATTTACAAACATATAAATTTTTTACAATCTATCAGTGATAGACCGCGATAGAATTCTATCACTTTCTATTGCTCAAGCAATAGAATTTATTTGCTTGAGTGATAGAAAGTGATAGAATTCTATCGCGGTCTGTCACTGTTAGACAGTGAAATTTTTCTATATTTGTAAATAGTTTGATATTTTTTTGTTTATAGTAATTTTCCTAAGTCTTACATAGCTAACTTAAAAAATGAGAAATGAAAAACGGGGAATTTCGTTTTTTAAGCTTTCAAAATCTTATTAAAATTTGTAGACGTGCCTTTTAAATTAATAAATTTTTAAACAAGGGGAATTGTTAATAAACGAACTTACAATATTAAAATAGTATAAAAAAAATAGAAGAAAGAACAAAATTAAAATCTCGTTTTAATAACCATTTTGAAAAATTAAGTGTATAAGCATCATTTTTAACTTGTTTTTATTTTTAATATTGGGCTAAAATTCAAATATTTTTTTAAGAAAGATAAATTTTATACTAAAAATTATTGAGAAAACAAATCCAACTTCTAAAGAAAATTGAAAATAAAAAATCAAATAGTAATTTAAATGAATCTTAATAAAATATAATCAAATAAAAGATGATACCGCAAAAGAAATATCATAATTAACATATGAAACCTACATTTTTTACGATTAAGAGGTTATAATTTCAATCTTCTTCTCCCAAAATTATTGCACTAAAAAGATAATATATATGAAAGGGAAAATTAACACATTTTTTTTTAAAGGAAGAAAACTTTTCTTTCACATCTAACATTTCCTTTCAAGTATATAATAAATTACAAAATCGTGCATTTTTCTTTTTTCCAATATAAGAAATTGCCAAATTACCAAATTGGTATGGTAATAATACCCACCAAACTTGTCATTGTCATTGCATCTACCAATATATAATATGTTCATTTTTTTTCTTCTCTTTATCTAGTTAATCAATTTTGTGGAGTAGTACTATTAATTTTGGATTAATTAGCTTTATTTATTTGTCTAATTAAATATGAAATTATCGCCTAAACAACTTTCTAGTATTAGCTTTAGAATTGCATCTAGATATCAACAATTCTAAAAGGTTTTTTTTTTTTTTGTTTCGTTTGTCTTGTTCATGGTTAGTTGTACCACCACTATTCAATTAAAAAAAAAAAATTCAATACACACGTAAAAATTTTAAGGTTACACTTTTTTTAAGTACAACGAATGGAGATATGAAACTTTGAGCCTCCAATTTTTGGAAAAGAATATATATGTCACTTACCGCTGAGATATCCTAACTTTAGCAATGTTGTATTTTGTAACAAATTAAGTAAAATTTTATTCTATTATGAGATACCATATGGTTTACAAAGTTTTCTGACATGGTTTTTTTTTTTTTTTTAATAGGAGCTATTATCGTTGCACTAGTGTCGCATGCAATGTAAAAAAACGGGTGGAGAGATGTTTGCAAGATTCAAGCATTGTCGTAACAACATATGAAGGCCAACACACTCACCCTAGCCCCATTATGGCACGATCAACCTTCTTTCCTCCACCCATCTCAGTCACCCTCTACGATGGTTATTCATATCAAAATAGTCATAGTTCAAATTTTATCAACGACCCAAAGGGCTTTGTATCATCATCGTTTCATCAACCGTGTGGCATTGCTTCCTCCAATCGAGCTACTCAATTGTTGGGTGCCACTATTGACCATGGACTACTTCAAGATATCACTCCTTTCCAGTTTGATGAGTTGGCATGGAGAGATGGTCCGATCTGA

mRNA sequence

ATGGAGGAGGTGGCGGCAGCCAATTCGTTGCGATATCAGTTTCTCGATGATGACGAGAACAAGAATCATCTAGGGTTCATGGAGCTTCTCAGTGTGGATCAAGATTTCTCATCGCAATTTGATGTCTTTGAAACATCATCGTCTTTATCGTCTTCCCTGATTTCAAATCCTGTTATGAACTCTGAGAATTTGGAGATTTGGAATCAATGGCCGACCACACCGAATTACTCGTCGTCGATCTCATCCACCTCGAGTGAGATCGTCAATGGCGAGCCGACTAAGCTAAATCAAGAGGGAGGAGAGAAACAACATCGCCAACAAACTGTAAAAACTAACAAGCAGTTGAAAACAAAGAAGACGAGTACAAAGAAGAAAGATCAAGAACAACGATTCGCTTTCATGACAAAGAGTGAAGTTGATCATTTGGAAGATGGATATAGATGGAGAAAGTATGGTCAAAAAGCAGTGAAAAACAGCCCTTATCCTAGGAGCTATTATCGTTGCACTAGTGTCGCATGCAATGTAAAAAAACGGGTGGAGAGATGTTTGCAAGATTCAAGCATTGTCGTAACAACATATGAAGGCCAACACACTCACCCTAGCCCCATTATGGCACGATCAACCTTCTTTCCTCCACCCATCTCAGTCACCCTCTACGATGGTTATTCATATCAAAATAGTCATAGTTCAAATTTTATCAACGACCCAAAGGGCTTTGTATCATCATCGTTTCATCAACCGTGTGGCATTGCTTCCTCCAATCGAGCTACTCAATTGTTGGGTGCCACTATTGACCATGGACTACTTCAAGATATCACTCCTTTCCAGTTTGATGAGTTGGCATGGAGAGATGGTCCGATCTGA

Coding sequence (CDS)

ATGGAGGAGGTGGCGGCAGCCAATTCGTTGCGATATCAGTTTCTCGATGATGACGAGAACAAGAATCATCTAGGGTTCATGGAGCTTCTCAGTGTGGATCAAGATTTCTCATCGCAATTTGATGTCTTTGAAACATCATCGTCTTTATCGTCTTCCCTGATTTCAAATCCTGTTATGAACTCTGAGAATTTGGAGATTTGGAATCAATGGCCGACCACACCGAATTACTCGTCGTCGATCTCATCCACCTCGAGTGAGATCGTCAATGGCGAGCCGACTAAGCTAAATCAAGAGGGAGGAGAGAAACAACATCGCCAACAAACTGTAAAAACTAACAAGCAGTTGAAAACAAAGAAGACGAGTACAAAGAAGAAAGATCAAGAACAACGATTCGCTTTCATGACAAAGAGTGAAGTTGATCATTTGGAAGATGGATATAGATGGAGAAAGTATGGTCAAAAAGCAGTGAAAAACAGCCCTTATCCTAGGAGCTATTATCGTTGCACTAGTGTCGCATGCAATGTAAAAAAACGGGTGGAGAGATGTTTGCAAGATTCAAGCATTGTCGTAACAACATATGAAGGCCAACACACTCACCCTAGCCCCATTATGGCACGATCAACCTTCTTTCCTCCACCCATCTCAGTCACCCTCTACGATGGTTATTCATATCAAAATAGTCATAGTTCAAATTTTATCAACGACCCAAAGGGCTTTGTATCATCATCGTTTCATCAACCGTGTGGCATTGCTTCCTCCAATCGAGCTACTCAATTGTTGGGTGCCACTATTGACCATGGACTACTTCAAGATATCACTCCTTTCCAGTTTGATGAGTTGGCATGGAGAGATGGTCCGATCTGA

Protein sequence

MEEVAAANSLRYQFLDDDENKNHLGFMELLSVDQDFSSQFDVFETSSSLSSSLISNPVMNSENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTKLNQEGGEKQHRQQTVKTNKQLKTKKTSTKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVERCLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISVTLYDGYSYQNSHSSNFINDPKGFVSSSFHQPCGIASSNRATQLLGATIDHGLLQDITPFQFDELAWRDGPI
Homology
BLAST of Lsi02G024900 vs. ExPASy Swiss-Prot
Match: Q9FGZ4 (Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 PE=2 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-44
Identity = 130/308 (42.21%), Postives = 172/308 (55.84%), Query Frame = 0

Query: 12  YQFLDDDENKNHLGFMELLSVDQDFSS-------QFDVF--ETSSSLSSSLISNPVMNSE 71
           Y + +D  + N   F++LL  D  F+S        FD F    +++ +S     P+  +E
Sbjct: 80  YSYFEDHSSNNPNSFLDLLRQDHQFASSSNSSSFSFDAFPLPNNNNNTSFFTDLPLPQAE 139

Query: 72  NLEIWNQWPTTPNYSSSISSTSSEIVNG-----EPTKLNQEGGEKQHRQQTVKTNKQLKT 131
           + E+ N  PT+PN S+S+SS+S+E  N      E T  +QE G++Q  Q+   T  QLK 
Sbjct: 140 SSEVVNTTPTSPN-STSVSSSSNEAANDNNSGKEVTVKDQEEGDQQQEQK--GTKPQLKA 199

Query: 132 KKTSTKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKK 191
           KK   +KK +E RFAF+TKS++D+L+DGYRWRKYGQKAVKNSPYPRSYYRCT+V C VKK
Sbjct: 200 KK-KNQKKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKK 259

Query: 192 RVERCLQDSSIVVTTYEGQHTHPSPIMAR--------------------STFFPPPISVT 251
           RVER   D SIV+TTYEGQHTHP P+  R                    S+F  P     
Sbjct: 260 RVERSSDDPSIVMTTYEGQHTHPFPMTPRGHIGMLTSPILDHGATTASSSSFSIPQPRYL 319

Query: 252 L---YDGYSYQNSHSSNFINDPKG---FVS---SSFHQPCGIASSNRATQLLGATIDHGL 277
           L   +  Y+  N++S + IN       FV+   SS     G   S  +T    +  DHGL
Sbjct: 320 LTQHHQPYNMYNNNSLSMINRRSSDGTFVNPGPSSSFPGFGYDMSQASTSTSSSIRDHGL 379

BLAST of Lsi02G024900 vs. ExPASy Swiss-Prot
Match: O22900 (WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 5.6e-41
Identity = 128/258 (49.61%), Postives = 151/258 (58.53%), Query Frame = 0

Query: 21  KNHLGFMELLSVD--QDF------------SSQFDVFETSSSLSSSLISNP--------- 80
           ++ LGFMELLS    QDF            S      + S+ LSSS+I  P         
Sbjct: 30  RHSLGFMELLSSQQHQDFATVSPHSFLLQTSQPQTQTQPSAKLSSSIIQAPPSEQLVTSK 89

Query: 81  --VMNSENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTKL---NQEGGEKQHRQQTVKTN 140
              + S++L I    P TPN SSSISS SSE +N E  K     +EGGE Q  Q+   T 
Sbjct: 90  VESLCSDHLLI--NPPATPN-SSSISSASSEALNEEKPKTEDNEEEGGEDQ--QEKSHTK 149

Query: 141 KQLKTKKTSTKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVA 200
           KQLK KK + +K+ +E R AFMTKSEVDHLEDGYRWRKYGQKAVKNSP+PRSYYRCT+ +
Sbjct: 150 KQLKAKK-NNQKRQREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTAS 209

Query: 201 CNVKKRVERCLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISVTLYDGYSYQNSHSSN- 247
           CNVKKRVER  +D S VVTTYEGQHTH SP+ +R      PIS   + G S   S   N 
Sbjct: 210 CNVKKRVERSFRDPSTVVTTYEGQHTHISPLTSR------PISTGGFFGSSGAASSLGNG 269

BLAST of Lsi02G024900 vs. ExPASy Swiss-Prot
Match: Q93WV4 (WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.4e-36
Identity = 109/262 (41.60%), Postives = 147/262 (56.11%), Query Frame = 0

Query: 31  SVDQDFSSQFDVFETSSSLSSSLISNPVMNSENLEIWNQWPTTPNYSSSISSTSSEIVNG 90
           S+  D++S   VF+ S   S     +P +N+  L + +  P        +SS+S+E   G
Sbjct: 28  SLVMDYNSLEKVFKFSPYSSPFQSVSPSVNNPYLNLTSNSPV-------VSSSSNE---G 87

Query: 91  EPTKLNQEGGEKQHRQQ-----TVKTNKQL-KTKKTSTKKKDQEQRFAFMTKSEVDHLED 150
           EP +   +  ++    +       +++KQL K  K   +KK++E R AFMTKSE+DHLED
Sbjct: 88  EPKENTNDKSDQMEDNEGDLHGVGESSKQLTKQGKKKGEKKEREVRVAFMTKSEIDHLED 147

Query: 151 GYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVERCLQDSSIVVTTYEGQHTHPSPIM 210
           GYRWRKYGQKAVKNSPYPRSYYRCT+  CNVKKRVER  QD SIV+TTYEG+H HP P  
Sbjct: 148 GYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPSIVITTYEGKHNHPIPST 207

Query: 211 ARSTFFPPPISVTLYDGYSYQNS---HSSNFI---NDPKGFVS-SSFHQPCGIASSNRAT 270
            R T     + V    G S  +S   H  +F+   + P  + S  S     G  +S+   
Sbjct: 208 LRGTVAAEHLLVHRGGGGSLLHSFPRHHQDFLMMKHSPANYQSVGSLSYEHGHGTSSYNF 267

Query: 271 QLLGATIDHGLLQDITPFQFDE 280
                 +D+GLLQDI P  F +
Sbjct: 268 NNNQPVVDYGLLQDIVPSMFSK 279

BLAST of Lsi02G024900 vs. ExPASy Swiss-Prot
Match: Q9FL26 (WRKY transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=WRKY8 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.9e-33
Identity = 83/145 (57.24%), Postives = 96/145 (66.21%), Query Frame = 0

Query: 97  QEGGEKQHRQQTVKTNKQLKTKKTSTKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAV 156
           ++GGE   R Q     K +KTKK   KKK  E R +FMTK+EVDHLEDGYRWRKYGQKAV
Sbjct: 144 RDGGEDDQRSQ-----KVVKTKKKEEKKK--EPRVSFMTKTEVDHLEDGYRWRKYGQKAV 203

Query: 157 KNSPYPRSYYRCTSVACNVKKRVERCLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISV 216
           KNSPYPRSYYRCT+  CNVKKRVER  QD ++V+TTYE QH HP P   R+  F    S 
Sbjct: 204 KNSPYPRSYYRCTTQKCNVKKRVERSYQDPTVVITTYESQHNHPIPTNRRTAMF----SG 263

Query: 217 TLYDGYSYQNS--HSSNFINDPKGF 240
           T    Y+  +S   S   IN P+ F
Sbjct: 264 TTASDYNPSSSPIFSDLIINTPRSF 277

BLAST of Lsi02G024900 vs. ExPASy Swiss-Prot
Match: Q8VWJ2 (WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-31
Identity = 85/174 (48.85%), Postives = 107/174 (61.49%), Query Frame = 0

Query: 47  SSLSSSLISNPVMNSENLEIWNQW-------PTTPNYSSSISSTSSEIVNGEPTKLNQEG 106
           S  SS + ++ +    N ++ N          T    S S SS+S     GE      + 
Sbjct: 71  SPSSSEVFNSSIDQEPNRDVTNDVINGGACNETETRVSPSNSSSSEADHPGE------DS 130

Query: 107 GEKQHRQQTVKTNKQL-----KTKKTSTKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQK 166
           G+ + +++ V    Q+     KTKKT  KK+ +E R +FMTKSEVDHLEDGYRWRKYGQK
Sbjct: 131 GKSRRKRELVGEEDQISKKVGKTKKTEVKKQ-REPRVSFMTKSEVDHLEDGYRWRKYGQK 190

Query: 167 AVKNSPYPRSYYRCTSVACNVKKRVERCLQDSSIVVTTYEGQHTHPSPIMARST 209
           AVKNSPYPRSYYRCT+  CNVKKRVER  QD ++V+TTYEGQH HP P   R +
Sbjct: 191 AVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPTVVITTYEGQHNHPIPTNLRGS 237

BLAST of Lsi02G024900 vs. ExPASy TrEMBL
Match: A0A6J1JVC0 (probable WRKY transcription factor 23 OS=Cucurbita maxima OX=3661 GN=LOC111488179 PE=4 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.9e-108
Identity = 216/298 (72.48%), Postives = 240/298 (80.54%), Query Frame = 0

Query: 1   MEEVAAANSLRYQFLDDDENKNHLGFMELLSVDQDFSSQFDVFETSSSLSSSLISNPVMN 60
           MEEVAAA+SLR+ F    E+K +LGFME+L VDQ+F SQFDVF T SSLSSSLISNP+  
Sbjct: 1   MEEVAAADSLRFPF---PEHKGYLGFMEMLDVDQEFLSQFDVFST-SSLSSSLISNPI-- 60

Query: 61  SENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTKLNQEGGEKQHRQQTVKTNKQLKTKKT 120
            ENLEIWNQWPTTPNYSSSISSTSSEIVN E  +   E  +KQH +QT+KT+KQ KT+KT
Sbjct: 61  -ENLEIWNQWPTTPNYSSSISSTSSEIVNDEVIEPKLEREKKQHSRQTLKTDKQSKTRKT 120

Query: 121 STKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVE 180
           S KKKDQE RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCT+VACNVKKRVE
Sbjct: 121 SRKKKDQEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTNVACNVKKRVE 180

Query: 181 RCLQDSSIVVTTYEGQHTHPSP-IMARSTFFPPPISVTLYDG-------------YSYQN 240
           R L+DSSIVVTTYEGQHTH SP  MAR+TFFPPPIS  LY+G             + YQN
Sbjct: 181 RYLKDSSIVVTTYEGQHTHSSPNNMARTTFFPPPISTALYNGSAMFTTATTTPSLFHYQN 240

Query: 241 SHSSNFINDPKGFVSSSFHQPCGIASSNRATQLLGATIDHGLLQDITPFQFDELAWRD 285
            H+SNFIN PK FV+SSFH PC +ASSN A Q   ATI+H L QDI PFQ++ELAWRD
Sbjct: 241 GHNSNFINHPKSFVASSFHPPCDVASSNHANQFFAATINHRLFQDIAPFQYNELAWRD 291

BLAST of Lsi02G024900 vs. ExPASy TrEMBL
Match: A0A6J1GMG2 (probable WRKY transcription factor 23 OS=Cucurbita moschata OX=3662 GN=LOC111455851 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 3.5e-102
Identity = 206/297 (69.36%), Postives = 234/297 (78.79%), Query Frame = 0

Query: 1   MEEVAAANSLRYQFLDDDENKNHLGFMELLSVDQDFSSQFDVFETSSSLSSSLISNPVMN 60
           M+EVAAA+SL++ FL   EN++ L FMEL++VDQ+FSSQFDVF + SSLSSSLI+NPV  
Sbjct: 1   MDEVAAADSLQFPFL---ENQSCLRFMELIAVDQEFSSQFDVF-SLSSLSSSLITNPV-- 60

Query: 61  SENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTKLNQEGGEKQHRQQTVKTNKQLKTKKT 120
            ENLEI NQWPTT NYSS     SSEIVN E  +   E  EKQH +QT+KT+KQ+KTKK 
Sbjct: 61  -ENLEIGNQWPTTSNYSS-----SSEIVNDEVIEPKLEREEKQHSRQTLKTDKQMKTKKM 120

Query: 121 STKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVE 180
           + KKKDQE RFAFMTKSEVDHLEDGYRWRKYGQKAVKN+PYPRSYYRCTSVACNVKKRVE
Sbjct: 121 TRKKKDQEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNNPYPRSYYRCTSVACNVKKRVE 180

Query: 181 RCLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISVTLYDG-------------YSYQNS 240
           R L+D SIVVTTYEGQHTHPSP M R+TFFPPPIS TLY+G             + YQN 
Sbjct: 181 RYLKDPSIVVTTYEGQHTHPSPNMTRTTFFPPPISTTLYNGSAMFTTATTTPSLFHYQNG 240

Query: 241 HSSNFINDPKGFVSSSFHQPCGIASSNRATQLLGATIDHGLLQDITPFQFDELAWRD 285
           H+SNFIN PKGFV+SSFH PC +A SN A Q   ATI+H L QDI PFQ++EL WRD
Sbjct: 241 HNSNFINHPKGFVASSFHPPCDVAPSNHANQFFAATINHRLFQDIAPFQYEELTWRD 285

BLAST of Lsi02G024900 vs. ExPASy TrEMBL
Match: A0A1S3BQY1 (probable WRKY transcription factor 23 OS=Cucumis melo OX=3656 GN=LOC103492743 PE=4 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 2.9e-96
Identity = 196/236 (83.05%), Postives = 207/236 (87.71%), Query Frame = 0

Query: 1   MEEVAAANSLRYQFLDDDENKNHLGFMELLSVDQDFSSQFDVFETSS-SLSSSLISNPVM 60
           MEEV AA+SLRY FLD +E+K+ LGFMELL VD+DFSSQFDVFETSS SLSSSLISNP  
Sbjct: 1   MEEVVAADSLRYPFLDGNESKSCLGFMELLEVDRDFSSQFDVFETSSPSLSSSLISNP-- 60

Query: 61  NSENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTKLNQEGG-EKQHRQQTVKTNKQLKTK 120
             ENLEIWNQWPTTPNYSSSISSTSSEIVNGE T+ N EGG EKQ RQ TVKT+KQLKTK
Sbjct: 61  --ENLEIWNQWPTTPNYSSSISSTSSEIVNGELTEPNLEGGEEKQDRQPTVKTDKQLKTK 120

Query: 121 KTSTKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKR 180
           K S KKK  E RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSP+PRSYYRCTSVAC VKKR
Sbjct: 121 KRSPKKKGVEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPHPRSYYRCTSVACQVKKR 180

Query: 181 VERCLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISVTLYDGYSYQNSHSSNFIN 235
           VERC QD SIVVTTYEGQHTHPSPIMAR TFFPPPISVTLYD Y  QNSH+SN ++
Sbjct: 181 VERCFQDPSIVVTTYEGQHTHPSPIMARPTFFPPPISVTLYDDYLIQNSHNSNVMS 232

BLAST of Lsi02G024900 vs. ExPASy TrEMBL
Match: E7CEY6 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY52 PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 3.0e-93
Identity = 195/238 (81.93%), Postives = 207/238 (86.97%), Query Frame = 0

Query: 1   MEEVAAANSLRYQFLDDDENKNHLG-FMELLSVDQDFSSQFDVFETSS-SLSSSLISNPV 60
           MEEV AA+SLRY FLD  ++K+ LG FMELL VDQ FSSQFDVFETSS SLSSSLISNP 
Sbjct: 1   MEEVVAADSLRYPFLDASDSKSCLGTFMELLEVDQHFSSQFDVFETSSPSLSSSLISNP- 60

Query: 61  MNSENLEIWNQWPTTPNYSSSISSTSSEIVNGE-PTKLNQEGGE-KQHRQQTVKTNKQLK 120
              ENLEIWNQWPTTPNYSSSISSTSSEIVNGE  T+ N EGGE KQ +Q TVK +KQLK
Sbjct: 61  ---ENLEIWNQWPTTPNYSSSISSTSSEIVNGELTTEPNLEGGEQKQDQQPTVKADKQLK 120

Query: 121 TKKTSTKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVK 180
           TKK S KKK  E RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSP+PRSYYRCTSVACNVK
Sbjct: 121 TKKRSPKKKGAEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPHPRSYYRCTSVACNVK 180

Query: 181 KRVERCLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISVTLYDGYSYQNSHSSNFIN 235
           KRVERCLQD SIVVTTYEGQHTHPSPIMARSTFFPPPIS TLY+ YS QNSH+SN ++
Sbjct: 181 KRVERCLQDPSIVVTTYEGQHTHPSPIMARSTFFPPPISATLYNDYSIQNSHNSNVMS 234

BLAST of Lsi02G024900 vs. ExPASy TrEMBL
Match: A0A3Q9R115 (WRKY transcription factor OS=Siraitia grosvenorii OX=190515 GN=WRKY17 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 3.3e-84
Identity = 189/309 (61.17%), Postives = 220/309 (71.20%), Query Frame = 0

Query: 1   MEEVAAANSLRYQFLDD----DENKNHLGFMELLSVDQDFSSQFDVFETSSSLSSSLISN 60
           MEE+A A+SLR  F       D+ K  LGFMELL V QDFSSQFD+F+T+S  SSSL  N
Sbjct: 3   MEEMAGADSLRCPFPGAVEFFDDGKGRLGFMELLGV-QDFSSQFDMFQTTSPPSSSLAPN 62

Query: 61  PVMNS--ENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTKLNQEGGEKQHRQQTVKTNKQ 120
             ++S  EN EIWN+WP TPN SSSISSTSSE VN E T+ NQ+GG+KQ RQ+ VK++KQ
Sbjct: 63  VTVDSVMENSEIWNRWPATPN-SSSISSTSSEAVNDEQTEQNQDGGDKQRRQEPVKSDKQ 122

Query: 121 LKTKKTSTKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACN 180
            K KKT+ KKK+QE RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACN
Sbjct: 123 WKAKKTNQKKKEQEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACN 182

Query: 181 VKKRVERCLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISVTLYDG------------- 240
           VKKRVERCL+D SIVVTTYEGQHTHPSP++ RSTF PPPIS  LY G             
Sbjct: 183 VKKRVERCLKDPSIVVTTYEGQHTHPSPVLPRSTFLPPPISAALYGGSATFATPCPTTTP 242

Query: 241 ---YSYQNSHSSNFI-NDPKGFVSSSFHQ---PCGIASSNRATQLLGATIDHGLLQDITP 284
              + YQN  +S+F+   P    + SFHQ   P G+ S++       A  DHGLLQD+  
Sbjct: 243 PSLFHYQN--ASDFVGGHPYALSAPSFHQDRRPRGVPSAHLLA--AAAAADHGLLQDVV- 302

BLAST of Lsi02G024900 vs. NCBI nr
Match: XP_038897173.1 (probable WRKY transcription factor 48 [Benincasa hispida])

HSP 1 Score: 478.4 bits (1230), Expect = 4.3e-131
Identity = 242/286 (84.62%), Postives = 259/286 (90.56%), Query Frame = 0

Query: 2   EEVAAANSLRYQFLDDDENKNHLGFMELLSVDQDFSSQFDVFETSSSLSSSLISNPVMNS 61
           EEVAAA+SLRY F D DENK+ +GFMELLSVDQDFS QFD+FETS SLSSSLISNPV+NS
Sbjct: 4   EEVAAADSLRYPFPDYDENKSRIGFMELLSVDQDFSLQFDMFETSMSLSSSLISNPVINS 63

Query: 62  ENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTKLNQEGGEKQHRQQTVKTNKQLKTKKTS 121
           ENLEIWNQWPTTPNYSSSISSTSSEIVNGEPT+ N EGGEKQHRQ TVK +KQLKTKKTS
Sbjct: 64  ENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTEPNLEGGEKQHRQPTVKVDKQLKTKKTS 123

Query: 122 TKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVER 181
            KKKDQE RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSP+PRSYYRCTSVACNVKKRVER
Sbjct: 124 PKKKDQEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPHPRSYYRCTSVACNVKKRVER 183

Query: 182 CLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISVTLYDGYSYQNSHSSNFINDPKGFVS 241
           CL D SIVVTTYEGQH+HPSPIMAR TFFPPPISVT Y+ + YQN HSSN I+ PKGF+ 
Sbjct: 184 CLNDPSIVVTTYEGQHSHPSPIMARPTFFPPPISVTHYNDHLYQNGHSSNIISHPKGFLP 243

Query: 242 SSFHQPCGIASSNRATQLLGATIDHGLLQDITPFQFDELAWRDGPI 288
           SSF+QPCGIASSNRATQLLG T++HGLLQDITP Q+D LAW DG I
Sbjct: 244 SSFYQPCGIASSNRATQLLGTTVNHGLLQDITPSQYDGLAWGDGSI 289

BLAST of Lsi02G024900 vs. NCBI nr
Match: XP_022991614.1 (probable WRKY transcription factor 23 [Cucurbita maxima])

HSP 1 Score: 402.1 bits (1032), Expect = 3.9e-108
Identity = 216/298 (72.48%), Postives = 240/298 (80.54%), Query Frame = 0

Query: 1   MEEVAAANSLRYQFLDDDENKNHLGFMELLSVDQDFSSQFDVFETSSSLSSSLISNPVMN 60
           MEEVAAA+SLR+ F    E+K +LGFME+L VDQ+F SQFDVF T SSLSSSLISNP+  
Sbjct: 1   MEEVAAADSLRFPF---PEHKGYLGFMEMLDVDQEFLSQFDVFST-SSLSSSLISNPI-- 60

Query: 61  SENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTKLNQEGGEKQHRQQTVKTNKQLKTKKT 120
            ENLEIWNQWPTTPNYSSSISSTSSEIVN E  +   E  +KQH +QT+KT+KQ KT+KT
Sbjct: 61  -ENLEIWNQWPTTPNYSSSISSTSSEIVNDEVIEPKLEREKKQHSRQTLKTDKQSKTRKT 120

Query: 121 STKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVE 180
           S KKKDQE RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCT+VACNVKKRVE
Sbjct: 121 SRKKKDQEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTNVACNVKKRVE 180

Query: 181 RCLQDSSIVVTTYEGQHTHPSP-IMARSTFFPPPISVTLYDG-------------YSYQN 240
           R L+DSSIVVTTYEGQHTH SP  MAR+TFFPPPIS  LY+G             + YQN
Sbjct: 181 RYLKDSSIVVTTYEGQHTHSSPNNMARTTFFPPPISTALYNGSAMFTTATTTPSLFHYQN 240

Query: 241 SHSSNFINDPKGFVSSSFHQPCGIASSNRATQLLGATIDHGLLQDITPFQFDELAWRD 285
            H+SNFIN PK FV+SSFH PC +ASSN A Q   ATI+H L QDI PFQ++ELAWRD
Sbjct: 241 GHNSNFINHPKSFVASSFHPPCDVASSNHANQFFAATINHRLFQDIAPFQYNELAWRD 291

BLAST of Lsi02G024900 vs. NCBI nr
Match: KAG6575751.1 (WRKY transcription factor 23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 382.5 bits (981), Expect = 3.2e-102
Identity = 209/297 (70.37%), Postives = 235/297 (79.12%), Query Frame = 0

Query: 1   MEEVAAANSLRYQFLDDDENKNHLGFMELLSVDQDFSSQFDVFETSSSLSSSLISNPVMN 60
           M+EVAAA+SLR+ FL   EN++ LGFME+L+VDQ+FSSQFDVF + SSLSSSLI+NPV  
Sbjct: 1   MDEVAAADSLRFPFL---ENQSCLGFMEMLAVDQEFSSQFDVF-SLSSLSSSLITNPV-- 60

Query: 61  SENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTKLNQEGGEKQHRQQTVKTNKQLKTKKT 120
            ENLEI NQWPTTPNYSS     SSEIVN E  +   E  EKQH +QT+KT+KQ+KTKK 
Sbjct: 61  -ENLEIENQWPTTPNYSS-----SSEIVNDEVIEPKLEREEKQHSRQTLKTDKQMKTKKM 120

Query: 121 STKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVE 180
           + KKKDQE RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVE
Sbjct: 121 TRKKKDQEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVE 180

Query: 181 RCLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISVTLYDG-------------YSYQNS 240
           R L+D SIVVTTYEGQHTHPSP M R+TFFPPPIS TLY+G             + YQN 
Sbjct: 181 RYLKDPSIVVTTYEGQHTHPSPNMTRTTFFPPPISTTLYNGSAMFTTATTTPSLFHYQNG 240

Query: 241 HSSNFINDPKGFVSSSFHQPCGIASSNRATQLLGATIDHGLLQDITPFQFDELAWRD 285
            +SNFIN PKGFV+SSFH PC +A SN A Q   ATI+H L QDI PFQ++ELA RD
Sbjct: 241 PNSNFINHPKGFVASSFHPPCDVAPSNHANQFFAATINHRLFQDIVPFQYEELAGRD 285

BLAST of Lsi02G024900 vs. NCBI nr
Match: XP_023549393.1 (probable WRKY transcription factor 23 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 381.7 bits (979), Expect = 5.5e-102
Identity = 209/297 (70.37%), Postives = 231/297 (77.78%), Query Frame = 0

Query: 1   MEEVAAANSLRYQFLDDDENKNHLGFMELLSVDQDFSSQFDVFETSSSLSSSLISNPVMN 60
           MEEVAAA+SLR+      EN++ L FMEL++VDQ+FSSQFDVF + SSLSSSL +NPV  
Sbjct: 1   MEEVAAADSLRFPL---PENQSCLKFMELIAVDQEFSSQFDVF-SLSSLSSSLFTNPV-- 60

Query: 61  SENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTKLNQEGGEKQHRQQTVKTNKQLKTKKT 120
            ENLEI NQWPTTPNYSS     SSEIVN E  +   E  EKQH +QT+KT+KQLKTKK 
Sbjct: 61  -ENLEIGNQWPTTPNYSS-----SSEIVNDEVIEPKLEREEKQHSRQTLKTDKQLKTKKM 120

Query: 121 STKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVE 180
           + KKKDQE RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVE
Sbjct: 121 TRKKKDQEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVE 180

Query: 181 RCLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISVTLYDG-------------YSYQNS 240
           R L+D SIVVTTYEGQHTH SP M RSTFFPPPIS  LY+G             + YQN 
Sbjct: 181 RYLKDPSIVVTTYEGQHTHSSPNMTRSTFFPPPISTALYNGSTMFTTATTTPSLFHYQNG 240

Query: 241 HSSNFINDPKGFVSSSFHQPCGIASSNRATQLLGATIDHGLLQDITPFQFDELAWRD 285
           H+SNFIN PKGFV+SSFH PC +A SN A Q    TI+H LLQDI PFQ+DELAWRD
Sbjct: 241 HNSNFINHPKGFVASSFHPPCDVAPSNHANQFFSPTINHRLLQDIAPFQYDELAWRD 285

BLAST of Lsi02G024900 vs. NCBI nr
Match: XP_022953246.1 (probable WRKY transcription factor 23 [Cucurbita moschata])

HSP 1 Score: 381.3 bits (978), Expect = 7.2e-102
Identity = 206/297 (69.36%), Postives = 234/297 (78.79%), Query Frame = 0

Query: 1   MEEVAAANSLRYQFLDDDENKNHLGFMELLSVDQDFSSQFDVFETSSSLSSSLISNPVMN 60
           M+EVAAA+SL++ FL   EN++ L FMEL++VDQ+FSSQFDVF + SSLSSSLI+NPV  
Sbjct: 1   MDEVAAADSLQFPFL---ENQSCLRFMELIAVDQEFSSQFDVF-SLSSLSSSLITNPV-- 60

Query: 61  SENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTKLNQEGGEKQHRQQTVKTNKQLKTKKT 120
            ENLEI NQWPTT NYSS     SSEIVN E  +   E  EKQH +QT+KT+KQ+KTKK 
Sbjct: 61  -ENLEIGNQWPTTSNYSS-----SSEIVNDEVIEPKLEREEKQHSRQTLKTDKQMKTKKM 120

Query: 121 STKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVE 180
           + KKKDQE RFAFMTKSEVDHLEDGYRWRKYGQKAVKN+PYPRSYYRCTSVACNVKKRVE
Sbjct: 121 TRKKKDQEPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNNPYPRSYYRCTSVACNVKKRVE 180

Query: 181 RCLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISVTLYDG-------------YSYQNS 240
           R L+D SIVVTTYEGQHTHPSP M R+TFFPPPIS TLY+G             + YQN 
Sbjct: 181 RYLKDPSIVVTTYEGQHTHPSPNMTRTTFFPPPISTTLYNGSAMFTTATTTPSLFHYQNG 240

Query: 241 HSSNFINDPKGFVSSSFHQPCGIASSNRATQLLGATIDHGLLQDITPFQFDELAWRD 285
           H+SNFIN PKGFV+SSFH PC +A SN A Q   ATI+H L QDI PFQ++EL WRD
Sbjct: 241 HNSNFINHPKGFVASSFHPPCDVAPSNHANQFFAATINHRLFQDIAPFQYEELTWRD 285

BLAST of Lsi02G024900 vs. TAIR 10
Match: AT5G49520.1 (WRKY DNA-binding protein 48 )

HSP 1 Score: 181.0 bits (458), Expect = 1.3e-45
Identity = 130/308 (42.21%), Postives = 172/308 (55.84%), Query Frame = 0

Query: 12  YQFLDDDENKNHLGFMELLSVDQDFSS-------QFDVF--ETSSSLSSSLISNPVMNSE 71
           Y + +D  + N   F++LL  D  F+S        FD F    +++ +S     P+  +E
Sbjct: 80  YSYFEDHSSNNPNSFLDLLRQDHQFASSSNSSSFSFDAFPLPNNNNNTSFFTDLPLPQAE 139

Query: 72  NLEIWNQWPTTPNYSSSISSTSSEIVNG-----EPTKLNQEGGEKQHRQQTVKTNKQLKT 131
           + E+ N  PT+PN S+S+SS+S+E  N      E T  +QE G++Q  Q+   T  QLK 
Sbjct: 140 SSEVVNTTPTSPN-STSVSSSSNEAANDNNSGKEVTVKDQEEGDQQQEQK--GTKPQLKA 199

Query: 132 KKTSTKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKK 191
           KK   +KK +E RFAF+TKS++D+L+DGYRWRKYGQKAVKNSPYPRSYYRCT+V C VKK
Sbjct: 200 KK-KNQKKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKK 259

Query: 192 RVERCLQDSSIVVTTYEGQHTHPSPIMAR--------------------STFFPPPISVT 251
           RVER   D SIV+TTYEGQHTHP P+  R                    S+F  P     
Sbjct: 260 RVERSSDDPSIVMTTYEGQHTHPFPMTPRGHIGMLTSPILDHGATTASSSSFSIPQPRYL 319

Query: 252 L---YDGYSYQNSHSSNFINDPKG---FVS---SSFHQPCGIASSNRATQLLGATIDHGL 277
           L   +  Y+  N++S + IN       FV+   SS     G   S  +T    +  DHGL
Sbjct: 320 LTQHHQPYNMYNNNSLSMINRRSSDGTFVNPGPSSSFPGFGYDMSQASTSTSSSIRDHGL 379

BLAST of Lsi02G024900 vs. TAIR 10
Match: AT2G47260.1 (WRKY DNA-binding protein 23 )

HSP 1 Score: 169.5 bits (428), Expect = 4.0e-42
Identity = 128/258 (49.61%), Postives = 151/258 (58.53%), Query Frame = 0

Query: 21  KNHLGFMELLSVD--QDF------------SSQFDVFETSSSLSSSLISNP--------- 80
           ++ LGFMELLS    QDF            S      + S+ LSSS+I  P         
Sbjct: 30  RHSLGFMELLSSQQHQDFATVSPHSFLLQTSQPQTQTQPSAKLSSSIIQAPPSEQLVTSK 89

Query: 81  --VMNSENLEIWNQWPTTPNYSSSISSTSSEIVNGEPTKL---NQEGGEKQHRQQTVKTN 140
              + S++L I    P TPN SSSISS SSE +N E  K     +EGGE Q  Q+   T 
Sbjct: 90  VESLCSDHLLI--NPPATPN-SSSISSASSEALNEEKPKTEDNEEEGGEDQ--QEKSHTK 149

Query: 141 KQLKTKKTSTKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTSVA 200
           KQLK KK + +K+ +E R AFMTKSEVDHLEDGYRWRKYGQKAVKNSP+PRSYYRCT+ +
Sbjct: 150 KQLKAKK-NNQKRQREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTAS 209

Query: 201 CNVKKRVERCLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISVTLYDGYSYQNSHSSN- 247
           CNVKKRVER  +D S VVTTYEGQHTH SP+ +R      PIS   + G S   S   N 
Sbjct: 210 CNVKKRVERSFRDPSTVVTTYEGQHTHISPLTSR------PISTGGFFGSSGAASSLGNG 269

BLAST of Lsi02G024900 vs. TAIR 10
Match: AT1G29860.1 (WRKY DNA-binding protein 71 )

HSP 1 Score: 154.1 bits (388), Expect = 1.7e-37
Identity = 109/262 (41.60%), Postives = 147/262 (56.11%), Query Frame = 0

Query: 31  SVDQDFSSQFDVFETSSSLSSSLISNPVMNSENLEIWNQWPTTPNYSSSISSTSSEIVNG 90
           S+  D++S   VF+ S   S     +P +N+  L + +  P        +SS+S+E   G
Sbjct: 28  SLVMDYNSLEKVFKFSPYSSPFQSVSPSVNNPYLNLTSNSPV-------VSSSSNE---G 87

Query: 91  EPTKLNQEGGEKQHRQQ-----TVKTNKQL-KTKKTSTKKKDQEQRFAFMTKSEVDHLED 150
           EP +   +  ++    +       +++KQL K  K   +KK++E R AFMTKSE+DHLED
Sbjct: 88  EPKENTNDKSDQMEDNEGDLHGVGESSKQLTKQGKKKGEKKEREVRVAFMTKSEIDHLED 147

Query: 151 GYRWRKYGQKAVKNSPYPRSYYRCTSVACNVKKRVERCLQDSSIVVTTYEGQHTHPSPIM 210
           GYRWRKYGQKAVKNSPYPRSYYRCT+  CNVKKRVER  QD SIV+TTYEG+H HP P  
Sbjct: 148 GYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPSIVITTYEGKHNHPIPST 207

Query: 211 ARSTFFPPPISVTLYDGYSYQNS---HSSNFI---NDPKGFVS-SSFHQPCGIASSNRAT 270
            R T     + V    G S  +S   H  +F+   + P  + S  S     G  +S+   
Sbjct: 208 LRGTVAAEHLLVHRGGGGSLLHSFPRHHQDFLMMKHSPANYQSVGSLSYEHGHGTSSYNF 267

Query: 271 QLLGATIDHGLLQDITPFQFDE 280
                 +D+GLLQDI P  F +
Sbjct: 268 NNNQPVVDYGLLQDIVPSMFSK 279

BLAST of Lsi02G024900 vs. TAIR 10
Match: AT5G46350.1 (WRKY DNA-binding protein 8 )

HSP 1 Score: 144.4 bits (363), Expect = 1.4e-34
Identity = 83/145 (57.24%), Postives = 96/145 (66.21%), Query Frame = 0

Query: 97  QEGGEKQHRQQTVKTNKQLKTKKTSTKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQKAV 156
           ++GGE   R Q     K +KTKK   KKK  E R +FMTK+EVDHLEDGYRWRKYGQKAV
Sbjct: 144 RDGGEDDQRSQ-----KVVKTKKKEEKKK--EPRVSFMTKTEVDHLEDGYRWRKYGQKAV 203

Query: 157 KNSPYPRSYYRCTSVACNVKKRVERCLQDSSIVVTTYEGQHTHPSPIMARSTFFPPPISV 216
           KNSPYPRSYYRCT+  CNVKKRVER  QD ++V+TTYE QH HP P   R+  F    S 
Sbjct: 204 KNSPYPRSYYRCTTQKCNVKKRVERSYQDPTVVITTYESQHNHPIPTNRRTAMF----SG 263

Query: 217 TLYDGYSYQNS--HSSNFINDPKGF 240
           T    Y+  +S   S   IN P+ F
Sbjct: 264 TTASDYNPSSSPIFSDLIINTPRSF 277

BLAST of Lsi02G024900 vs. TAIR 10
Match: AT4G18170.1 (WRKY DNA-binding protein 28 )

HSP 1 Score: 138.7 bits (348), Expect = 7.6e-33
Identity = 85/174 (48.85%), Postives = 107/174 (61.49%), Query Frame = 0

Query: 47  SSLSSSLISNPVMNSENLEIWNQW-------PTTPNYSSSISSTSSEIVNGEPTKLNQEG 106
           S  SS + ++ +    N ++ N          T    S S SS+S     GE      + 
Sbjct: 71  SPSSSEVFNSSIDQEPNRDVTNDVINGGACNETETRVSPSNSSSSEADHPGE------DS 130

Query: 107 GEKQHRQQTVKTNKQL-----KTKKTSTKKKDQEQRFAFMTKSEVDHLEDGYRWRKYGQK 166
           G+ + +++ V    Q+     KTKKT  KK+ +E R +FMTKSEVDHLEDGYRWRKYGQK
Sbjct: 131 GKSRRKRELVGEEDQISKKVGKTKKTEVKKQ-REPRVSFMTKSEVDHLEDGYRWRKYGQK 190

Query: 167 AVKNSPYPRSYYRCTSVACNVKKRVERCLQDSSIVVTTYEGQHTHPSPIMARST 209
           AVKNSPYPRSYYRCT+  CNVKKRVER  QD ++V+TTYEGQH HP P   R +
Sbjct: 191 AVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPTVVITTYEGQHNHPIPTNLRGS 237

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FGZ41.9e-4442.21Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 ... [more]
O229005.6e-4149.61WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1[more]
Q93WV42.4e-3641.60WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1[more]
Q9FL261.9e-3357.24WRKY transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=WRKY8 PE=1 SV=1[more]
Q8VWJ21.1e-3148.85WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JVC01.9e-10872.48probable WRKY transcription factor 23 OS=Cucurbita maxima OX=3661 GN=LOC11148817... [more]
A0A6J1GMG23.5e-10269.36probable WRKY transcription factor 23 OS=Cucurbita moschata OX=3662 GN=LOC111455... [more]
A0A1S3BQY12.9e-9683.05probable WRKY transcription factor 23 OS=Cucumis melo OX=3656 GN=LOC103492743 PE... [more]
E7CEY63.0e-9381.93WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY52 PE=2 SV=1[more]
A0A3Q9R1153.3e-8461.17WRKY transcription factor OS=Siraitia grosvenorii OX=190515 GN=WRKY17 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038897173.14.3e-13184.62probable WRKY transcription factor 48 [Benincasa hispida][more]
XP_022991614.13.9e-10872.48probable WRKY transcription factor 23 [Cucurbita maxima][more]
KAG6575751.13.2e-10270.37WRKY transcription factor 23, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023549393.15.5e-10270.37probable WRKY transcription factor 23 [Cucurbita pepo subsp. pepo][more]
XP_022953246.17.2e-10269.36probable WRKY transcription factor 23 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G49520.11.3e-4542.21WRKY DNA-binding protein 48 [more]
AT2G47260.14.0e-4249.61WRKY DNA-binding protein 23 [more]
AT1G29860.11.7e-3741.60WRKY DNA-binding protein 71 [more]
AT5G46350.11.4e-3457.24WRKY DNA-binding protein 8 [more]
AT4G18170.17.6e-3348.85WRKY DNA-binding protein 28 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 142..201
e-value: 1.3E-38
score: 144.3
IPR003657WRKY domainPFAMPF03106WRKYcoord: 143..200
e-value: 2.8E-25
score: 88.3
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 137..202
score: 31.157759
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 125..203
e-value: 1.2E-33
score: 116.8
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 134..202
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 78..92
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 78..128
NoneNo IPR availablePANTHERPTHR31221:SF238WRKY TRANSCRIPTION FACTOR 48-RELATEDcoord: 26..249
IPR044810WRKY transcription factor, plantPANTHERPTHR31221WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATEDcoord: 26..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G024900.1Lsi02G024900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding