Lsi02G024830 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G024830
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	mitogen-activated protein kinase kinase kinase ANP1-like
Location	chr02: 31276208 .. 31276852 (+)
RNA-Seq Expression	Lsi02G024830
Synteny	Lsi02G024830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATACTTAAAGGTCTTTCGTGTATCCATAGTAAAGGATTTGTTCATGTTGATCTTAAGCCGAGTAATATTCTTGTGTTCCCCCAAAGTAATGGGAAGATGAAATTGAAAATTGCCGACTTTGGAATTGGTGAGAGATGCAAACATAGGGATGATGATCAAGACATAAAGTATCGGCAACGCGGCCCATTGAAGTTTAAAAGAACACCTATATATGTGTCACCTGAATCCCTTGTTTTCAATGATGCACATGATATTTGGTCATTAGGTTGCATTTTGGTTAAAATGGTTTCCGGAAAGTCTGCGTGGGATGATTGTAGTGATGAACAATTGATGATAAGACTTTTGAATAATAAGAATATACCAACAATACCTATAGAATTATCCAACCAAGGCAAGGACTTTATTAAAAAATGTTTTGTTCGAAACTATAAACACCGTTGGACAGCTGATATGTTACTCCAACATCCCTATCTCAGTGAAGAAAATGATGCTACGAAGAGAAGTGATGAAAATGATGAAGCTACGAAGAGAAGTGATGAAAATGAAGCTGCGAAGAGAAGTGATGAGATGCTGGAAAATCCAATCCTCGAAAAGAAGATCATTTGCCTCATCATCTATTGTGCAAGATTAAATCAAATTTAG

mRNA sequence

ATGATACTTAAAGGTCTTTCGTGTATCCATAGTAAAGGATTTGTTCATGTTGATCTTAAGCCGAGTAATATTCTTGTGTTCCCCCAAAGTAATGGGAAGATGAAATTGAAAATTGCCGACTTTGGAATTGGTGAGAGATGCAAACATAGGGATGATGATCAAGACATAAAGTATCGGCAACGCGGCCCATTGAAGTTTAAAAGAACACCTATATATGTGTCACCTGAATCCCTTGTTTTCAATGATGCACATGATATTTGGTCATTAGGTTGCATTTTGGTTAAAATGGTTTCCGGAAAGTCTGCGTGGGATGATTGTAGTGATGAACAATTGATGATAAGACTTTTGAATAATAAGAATATACCAACAATACCTATAGAATTATCCAACCAAGGCAAGGACTTTATTAAAAAATGTTTTGTTCGAAACTATAAACACCGTTGGACAGCTGATATGTTACTCCAACATCCCTATCTCAGTGAAGAAAATGATGCTACGAAGAGAAGTGATGAAAATGATGAAGCTACGAAGAGAAGTGATGAAAATGAAGCTGCGAAGAGAAGTGATGAGATGCTGGAAAATCCAATCCTCGAAAAGAAGATCATTTGCCTCATCATCTATTGTGCAAGATTAAATCAAATTTAG

Coding sequence (CDS)

ATGATACTTAAAGGTCTTTCGTGTATCCATAGTAAAGGATTTGTTCATGTTGATCTTAAGCCGAGTAATATTCTTGTGTTCCCCCAAAGTAATGGGAAGATGAAATTGAAAATTGCCGACTTTGGAATTGGTGAGAGATGCAAACATAGGGATGATGATCAAGACATAAAGTATCGGCAACGCGGCCCATTGAAGTTTAAAAGAACACCTATATATGTGTCACCTGAATCCCTTGTTTTCAATGATGCACATGATATTTGGTCATTAGGTTGCATTTTGGTTAAAATGGTTTCCGGAAAGTCTGCGTGGGATGATTGTAGTGATGAACAATTGATGATAAGACTTTTGAATAATAAGAATATACCAACAATACCTATAGAATTATCCAACCAAGGCAAGGACTTTATTAAAAAATGTTTTGTTCGAAACTATAAACACCGTTGGACAGCTGATATGTTACTCCAACATCCCTATCTCAGTGAAGAAAATGATGCTACGAAGAGAAGTGATGAAAATGATGAAGCTACGAAGAGAAGTGATGAAAATGAAGCTGCGAAGAGAAGTGATGAGATGCTGGAAAATCCAATCCTCGAAAAGAAGATCATTTGCCTCATCATCTATTGTGCAAGATTAAATCAAATTTAG

Protein sequence

MILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQRGPLKFKRTPIYVSPESLVFNDAHDIWSLGCILVKMVSGKSAWDDCSDEQLMIRLLNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRSDENDEATKRSDENEAAKRSDEMLENPILEKKIICLIIYCARLNQI

Homology

[Close]

BLAST of Lsi02G024830 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.1e-28
Identity = 73/184 (39.67%), Postives = 109/184 (59.24%), Query Frame = 0


Query: 2   ILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQR 61
           +L+GL  IH+KGF H D+K +NIL+F   +    +KIADFG+  R      D D+    R
Sbjct: 115 VLRGLRHIHAKGFAHCDIKLANILLFNDGS----VKIADFGLAMRV-----DGDLT-ALR 174

Query: 62  GPLKFKRTPIYVSPESLVFND---AHDIWSLGCILVKMVSGKSAW---DDCSDEQLMIRL 121
             ++ + TP+Y++PE +  N+   A D+W+LGC +V+M SGK+AW   +      L+IR+
Sbjct: 175 KSVEIRGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRI 234

Query: 122 LNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLS-------EENDATKR 173
                +P IP  LS +GKDF+ KCFV++   RWTA+MLL H +++        EN   K 
Sbjct: 235 GVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKV 288

[Close]

BLAST of Lsi02G024830 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 5.0e-26
Identity = 64/162 (39.51%), Postives = 92/162 (56.79%), Query Frame = 0


Query: 2   ILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQR 61
           IL GL+ +H+K  VH D+K +NILV P      ++K+ADFG+ +              Q 
Sbjct: 509 ILSGLAYLHAKNTVHRDIKGANILVDPHG----RVKVADFGMAKHIT----------AQS 568

Query: 62  GPLKFKRTPIYVSPE----SLVFNDAHDIWSLGCILVKMVSGKSAWDDCSDEQLMIRLLN 121
           GPL FK +P +++PE    S   N A DIWSLGC +++M + K  W        M ++ N
Sbjct: 569 GPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFKIGN 628

Query: 122 NKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYL 160
           +K +P IP  LS +GKDF++KC  RN  +R TA  LL H ++
Sbjct: 629 SKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

[Close]

BLAST of Lsi02G024830 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.7e-21
Identity = 67/185 (36.22%), Postives = 97/185 (52.43%), Query Frame = 0


Query: 2   ILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQR 61
           IL GL+ +H +  VH D+K +NILV P  NG++KL  ADFG+    KH      +     
Sbjct: 323 ILAGLAYLHGRNTVHRDIKGANILVDP--NGEIKL--ADFGM---AKHVTAFSTM----- 382

Query: 62  GPLKFKRTPIYVSPESLV----FNDAHDIWSLGCILVKMVSGKSAWDDCSDEQLMIRLLN 121
             L FK +P +++PE ++    +  A DIWSLGC +++M + K  W        + ++ N
Sbjct: 383 --LSFKGSPYWMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWSQFEGVAAIFKIGN 442

Query: 122 NKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRSDENDEATK 181
           +K+ P IP  LSN  K+FI+ C  RN   R TA  LL+HP+L         S   D   +
Sbjct: 443 SKDTPEIPDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTRVASTSLPKDFPPR 493

Query: 182 RSDEN 183
             D N
Sbjct: 503 SYDGN 493

[Close]

BLAST of Lsi02G024830 vs. ExPASy Swiss-Prot
Match: Q9C5H5 (Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK5 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.3e-21
Identity = 64/181 (35.36%), Postives = 101/181 (55.80%), Query Frame = 0


Query: 2   ILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQR 61
           IL GL+ +H+K  VH D+K +N+LV   ++G +KL  ADFG+    KH          QR
Sbjct: 456 ILSGLAYLHNKKTVHRDIKGANLLV--DASGVVKL--ADFGM---AKHLTG-------QR 515

Query: 62  GPLKFKRTPIYVSPE---SLVFNDAH-------DIWSLGCILVKMVSGKSAWDDCSDEQL 121
             L  K +P +++PE   +++  D++       DIWSLGC +++M +GK  W +      
Sbjct: 516 ADLSLKGSPYWMAPELMQAVMQKDSNPDLAFAVDIWSLGCTIIEMFTGKPPWSEFEGAAA 575

Query: 122 MIRLLNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRSDE 173
           M +++  ++ P IP  +S +GKDF++ CF RN   R TA MLL+H +L      T  S+ 
Sbjct: 576 MFKVM--RDSPPIPESMSPEGKDFLRLCFQRNPAERPTASMLLEHRFLKNSLQPTSPSNS 620

[Close]

BLAST of Lsi02G024830 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.1e-20
Identity = 65/207 (31.40%), Postives = 102/207 (49.28%), Query Frame = 0


Query: 2   ILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQR 61
           ILKGL  IHSKG VH D+K SN+++    + K + KIADFG  +R      D   +    
Sbjct: 110 ILKGLEYIHSKGIVHCDVKGSNVVI----SEKGEAKIADFGCAKRV-----DPVFESPVM 169

Query: 62  GPLKFKRTPIYVSPE---SLVFNDAHDIWSLGCILVKMVSGKSAWDDCSDEQ----LMIR 121
           G      TP +++PE           DIW++GC +++MV+G   W      +    ++ R
Sbjct: 170 G------TPAFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYR 229

Query: 122 LLNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEEND---------- 181
           +  +   P +P  L+ + KDF++KC  R    RWTA  LL HP+L+ + D          
Sbjct: 230 VGYSSETPELPCLLAEEAKDFLEKCLKREANERWTATQLLNHPFLTTKPDIEPVLVPGLI 289

Query: 182 ATKRSDENDEATKRSDENEAAKRSDEM 192
           +   +   D+   RS E E  + ++E+
Sbjct: 290 SNSPTSVTDQTFWRSVEEEEEEETEEI 301

[Close]

BLAST of Lsi02G024830 vs. ExPASy TrEMBL
Match: A0A5D3DR57 (Mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00640 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.3e-66
Identity = 135/195 (69.23%), Postives = 155/195 (79.49%), Query Frame = 0


Query: 1   MILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQ 60
           MILKGLSCIHSKGFVHVDLKP+NIL FPQS+GKMKLKIADFG  ER K+R DDQ  +Y  
Sbjct: 123 MILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQYPP 182

Query: 61  -RGPLKFKRTPIYVSPESLVF---NDAHDIWSLGCILVKMVSGKSAWDDCSD-EQLMIRL 120
                KFK TP Y+SPES+VF   NDA DIWSLGCILVKM+SGKSAWDDC D +QLMI+L
Sbjct: 183 CCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMIKL 242

Query: 121 LNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRSDENDEA 180
           L+NK +PTIP ELS QGKDFIKKCF+RNYK RWTA+MLLQHPYL+EEN+A    +  +EA
Sbjct: 243 LDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENEAP--MNNKNEA 302

Query: 181 TKRSDENEAAKRSDE 191
           T + D     ++ DE
Sbjct: 303 TMKKDNEATMQKDDE 315

[Close]

BLAST of Lsi02G024830 vs. ExPASy TrEMBL
Match: A0A1S3CQQ0 (mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo OX=3656 GN=LOC103503238 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.3e-66
Identity = 135/195 (69.23%), Postives = 155/195 (79.49%), Query Frame = 0


Query: 1   MILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQ 60
           MILKGLSCIHSKGFVHVDLKP+NIL FPQS+GKMKLKIADFG  ER K+R DDQ  +Y  
Sbjct: 123 MILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQYPP 182

Query: 61  -RGPLKFKRTPIYVSPESLVF---NDAHDIWSLGCILVKMVSGKSAWDDCSD-EQLMIRL 120
                KFK TP Y+SPES+VF   NDA DIWSLGCILVKM+SGKSAWDDC D +QLMI+L
Sbjct: 183 CCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMIKL 242

Query: 121 LNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRSDENDEA 180
           L+NK +PTIP ELS QGKDFIKKCF+RNYK RWTA+MLLQHPYL+EEN+A    +  +EA
Sbjct: 243 LDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENEAP--MNNKNEA 302

Query: 181 TKRSDENEAAKRSDE 191
           T + D     ++ DE
Sbjct: 303 TMKKDNEATMQKDDE 315

[Close]

BLAST of Lsi02G024830 vs. ExPASy TrEMBL
Match: A0A0A0KAC2 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378450 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 2.1e-64
Identity = 128/186 (68.82%), Postives = 151/186 (81.18%), Query Frame = 0


Query: 1   MILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDD-QDIKYR 60
           MILKGLSCIHSKGFVHVDLKP+NIL FPQS+GKMKLKIADFG  ERCK+RDD+ Q  +Y 
Sbjct: 120 MILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERCKYRDDNGQHKRYG 179

Query: 61  QRGPLKFKRTPIYVSPESLVFN---DAHDIWSLGCILVKMVSGKSAWDDCSD-EQLMIRL 120
               LKFK +P Y+SPES++FN   DAHDIWSLGCILVKM+SGK  WD  +D +QLMI +
Sbjct: 180 YCSSLKFKGSPRYMSPESIIFNEVDDAHDIWSLGCILVKMISGKCVWDGYTDSKQLMIEV 239

Query: 121 LNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRSDENDEA 180
           L+NK + TIP ELS QGKDFI+KCF+R+YK RWTADMLLQHPYL++EN+A  +    DEA
Sbjct: 240 LDNKIMATIPGELSEQGKDFIRKCFIRSYKQRWTADMLLQHPYLNQENEAPMK---EDEA 299

Query: 181 TKRSDE 182
           T + DE
Sbjct: 300 TMKDDE 302

[Close]

BLAST of Lsi02G024830 vs. ExPASy TrEMBL
Match: A0A0A0K7G3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G065150 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.2e-62
Identity = 123/183 (67.21%), Postives = 151/183 (82.51%), Query Frame = 0


Query: 1   MILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRD------DDQ 60
           MILKGLSCIHSKGFVHVDLKP NIL FP+SNGKMKLKIADFG  ERCK+R+      DD+
Sbjct: 122 MILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADAHSDDE 181

Query: 61  DIKYRQRGP-LKFKRTPIYVSPESLVF---NDAHDIWSLGCILVKMVSGKSAWDD-CSDE 120
             K R+R   LKFK TP Y+SPES+VF   NDAHD+WSLGC+LV+M+SGKSAWD+  + +
Sbjct: 182 YRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVATSK 241

Query: 121 QLMIRLLNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRS 173
           QLM+++L++K++P IP ELS QGKDFIKKCFV N+K RWTADMLLQHPYL+E+++AT   
Sbjct: 242 QLMMKILDSKSMPRIPEELSIQGKDFIKKCFVWNFKQRWTADMLLQHPYLNEKHEATTMK 301

[Close]

BLAST of Lsi02G024830 vs. ExPASy TrEMBL
Match: A0A5D3C4R6 (Mitogen-activated protein kinase kinase kinase A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002080 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 3.4e-62
Identity = 131/187 (70.05%), Postives = 146/187 (78.07%), Query Frame = 0


Query: 1   MILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCK-----HRDDDQD 60
           MILKGLSC+HSKGFVHVDLKP+NIL FPQS+GKMKLKIADFG  ERCK       DDDQD
Sbjct: 120 MILKGLSCVHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGHAERCKLYRNDDDDDDQD 179

Query: 61  IKY-RQRGPLKFKRTPIYVSPESLV---FNDAHDIWSLGCILVKMVSGKSAWDDCSD-EQ 120
            KY      LKFK TP Y+SPES+V    NDAHDIWSLGC+LVKM+SGKS WDD  D  Q
Sbjct: 180 RKYGGYCSSLKFKGTPRYMSPESIVCNEVNDAHDIWSLGCVLVKMISGKSVWDDLIDSRQ 239

Query: 121 LMIRLLNNK--NIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYL-SEENDATK 175
           LM+RLL+NK  N+PTIP ELS QGKDFIKKCF+RN K RWTADMLLQHPYL  EEN+A  
Sbjct: 240 LMMRLLDNKNYNMPTIPKELSKQGKDFIKKCFIRNCKQRWTADMLLQHPYLMHEENEAAT 299

[Close]

BLAST of Lsi02G024830 vs. NCBI nr
Match: XP_038896325.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 273.5 bits (698), Expect = 1.6e-69
Identity = 132/175 (75.43%), Postives = 148/175 (84.57%), Query Frame = 0


Query: 1   MILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQ 60
           MILKGLSCIH +GFVHVDLKP+N+LVFPQS+GKMKLKIADFG+ ERCKH  DDQD  Y +
Sbjct: 117 MILKGLSCIHRRGFVHVDLKPANVLVFPQSDGKMKLKIADFGLAERCKHIVDDQDRGYLR 176

Query: 61  RGPLKFKRTPIYVSPESLVFND---AHDIWSLGCILVKMVSGKSAWDDC-SDEQLMIRLL 120
               KFK TP Y+SPES+ FN+    HDIWSLGCILVKMVSGKS WDDC   EQLM RLL
Sbjct: 177 ----KFKGTPKYMSPESIFFNEVNGTHDIWSLGCILVKMVSGKSVWDDCRGSEQLMTRLL 236

Query: 121 NNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRSDE 172
           N+KNIPTIP ELS QGKDFIK+CF+RNYK RWTADMLLQHPYL+EEN+ATK+ D+
Sbjct: 237 NDKNIPTIPKELSKQGKDFIKRCFIRNYKQRWTADMLLQHPYLNEENEATKKGDD 287

[Close]

BLAST of Lsi02G024830 vs. NCBI nr
Match: KAA0059705.1 (mitogen-activated protein kinase kinase kinase ANP1-like [Cucumis melo var. makuwa] >TYK26143.1 mitogen-activated protein kinase kinase kinase ANP1-like [Cucumis melo var. makuwa])

HSP 1 Score: 262.7 bits (670), Expect = 2.8e-66
Identity = 135/195 (69.23%), Postives = 155/195 (79.49%), Query Frame = 0


Query: 1   MILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQ 60
           MILKGLSCIHSKGFVHVDLKP+NIL FPQS+GKMKLKIADFG  ER K+R DDQ  +Y  
Sbjct: 123 MILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQYPP 182

Query: 61  -RGPLKFKRTPIYVSPESLVF---NDAHDIWSLGCILVKMVSGKSAWDDCSD-EQLMIRL 120
                KFK TP Y+SPES+VF   NDA DIWSLGCILVKM+SGKSAWDDC D +QLMI+L
Sbjct: 183 CCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMIKL 242

Query: 121 LNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRSDENDEA 180
           L+NK +PTIP ELS QGKDFIKKCF+RNYK RWTA+MLLQHPYL+EEN+A    +  +EA
Sbjct: 243 LDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENEAP--MNNKNEA 302

Query: 181 TKRSDENEAAKRSDE 191
           T + D     ++ DE
Sbjct: 303 TMKKDNEATMQKDDE 315

[Close]

BLAST of Lsi02G024830 vs. NCBI nr
Match: XP_008465601.1 (PREDICTED: mitogen-activated protein kinase kinase kinase ANP1-like, partial [Cucumis melo])

HSP 1 Score: 262.7 bits (670), Expect = 2.8e-66
Identity = 135/195 (69.23%), Postives = 155/195 (79.49%), Query Frame = 0


Query: 1   MILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQ 60
           MILKGLSCIHSKGFVHVDLKP+NIL FPQS+GKMKLKIADFG  ER K+R DDQ  +Y  
Sbjct: 123 MILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQYPP 182

Query: 61  -RGPLKFKRTPIYVSPESLVF---NDAHDIWSLGCILVKMVSGKSAWDDCSD-EQLMIRL 120
                KFK TP Y+SPES+VF   NDA DIWSLGCILVKM+SGKSAWDDC D +QLMI+L
Sbjct: 183 CCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMIKL 242

Query: 121 LNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRSDENDEA 180
           L+NK +PTIP ELS QGKDFIKKCF+RNYK RWTA+MLLQHPYL+EEN+A    +  +EA
Sbjct: 243 LDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENEAP--MNNKNEA 302

Query: 181 TKRSDENEAAKRSDE 191
           T + D     ++ DE
Sbjct: 303 TMKKDNEATMQKDDE 315

[Close]

BLAST of Lsi02G024830 vs. NCBI nr
Match: XP_031745125.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN44756.1 hypothetical protein Csa_016572 [Cucumis sativus])

HSP 1 Score: 255.4 bits (651), Expect = 4.4e-64
Identity = 128/186 (68.82%), Postives = 151/186 (81.18%), Query Frame = 0


Query: 1   MILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDD-QDIKYR 60
           MILKGLSCIHSKGFVHVDLKP+NIL FPQS+GKMKLKIADFG  ERCK+RDD+ Q  +Y 
Sbjct: 120 MILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERCKYRDDNGQHKRYG 179

Query: 61  QRGPLKFKRTPIYVSPESLVFN---DAHDIWSLGCILVKMVSGKSAWDDCSD-EQLMIRL 120
               LKFK +P Y+SPES++FN   DAHDIWSLGCILVKM+SGK  WD  +D +QLMI +
Sbjct: 180 YCSSLKFKGSPRYMSPESIIFNEVDDAHDIWSLGCILVKMISGKCVWDGYTDSKQLMIEV 239

Query: 121 LNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRSDENDEA 180
           L+NK + TIP ELS QGKDFI+KCF+R+YK RWTADMLLQHPYL++EN+A  +    DEA
Sbjct: 240 LDNKIMATIPGELSEQGKDFIRKCFIRSYKQRWTADMLLQHPYLNQENEAPMK---EDEA 299

Query: 181 TKRSDE 182
           T + DE
Sbjct: 300 TMKDDE 302

[Close]

BLAST of Lsi02G024830 vs. NCBI nr
Match: XP_004137063.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN43756.1 hypothetical protein Csa_017187 [Cucumis sativus])

HSP 1 Score: 249.6 bits (636), Expect = 2.4e-62
Identity = 123/183 (67.21%), Postives = 151/183 (82.51%), Query Frame = 0


Query: 1   MILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRD------DDQ 60
           MILKGLSCIHSKGFVHVDLKP NIL FP+SNGKMKLKIADFG  ERCK+R+      DD+
Sbjct: 122 MILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADAHSDDE 181

Query: 61  DIKYRQRGP-LKFKRTPIYVSPESLVF---NDAHDIWSLGCILVKMVSGKSAWDD-CSDE 120
             K R+R   LKFK TP Y+SPES+VF   NDAHD+WSLGC+LV+M+SGKSAWD+  + +
Sbjct: 182 YRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVATSK 241

Query: 121 QLMIRLLNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRS 173
           QLM+++L++K++P IP ELS QGKDFIKKCFV N+K RWTADMLLQHPYL+E+++AT   
Sbjct: 242 QLMMKILDSKSMPRIPEELSIQGKDFIKKCFVWNFKQRWTADMLLQHPYLNEKHEATTMK 301

[Close]

BLAST of Lsi02G024830 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 137.9 bits (346), Expect = 9.6e-33
Identity = 79/191 (41.36%), Postives = 114/191 (59.69%), Query Frame = 0


Query: 2   ILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQR 61
           I+KGL  IH  GF H D+K  N+LVF    G   +KI+DFG+ +R   R  +  ++ R  
Sbjct: 115 IVKGLCHIHGNGFTHCDIKLENVLVF----GDGDVKISDFGLAKR---RSGEVCVEIR-- 174

Query: 62  GPLKFKRTPIYVSPESL---VFNDAHDIWSLGCILVKMVSGKSAWDDCSDE-------QL 121
                  TP+Y++PES+    F    DIW+LGC +V+M SGK+AW  C ++        L
Sbjct: 175 ------GTPLYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAW--CLEDGVMNNVMSL 234

Query: 122 MIRLLNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRSDE 181
           ++R+ +   +P IP+ELS +GKDF+ KCFV+N   RWTA+MLL HP+L+ +++    S E
Sbjct: 235 LVRIGSGDEVPRIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDE----SGE 284

Query: 182 NDEATKRSDEN 183
            DEA   S  N
Sbjct: 295 EDEACSVSPRN 284

[Close]

BLAST of Lsi02G024830 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 132.1 bits (331), Expect = 5.3e-31
Identity = 74/187 (39.57%), Postives = 112/187 (59.89%), Query Frame = 0


Query: 2   ILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQR 61
           +L+GL  IH+ GF H DLK  NIL+F    G   +KIADFG+ +R     D   + Y   
Sbjct: 117 VLRGLRHIHANGFAHCDLKLGNILLF----GDGAVKIADFGLAKRI---GDLTALNY--- 176

Query: 62  GPLKFKRTPIYVSPESLVFND---AHDIWSLGCILVKMVSGKSAW---DDCSDEQLMIRL 121
             ++ + TP+Y++PES+  N+     D+W+LGC++V+M SGK+AW   +  +   L++R+
Sbjct: 177 -GVQIRGTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRI 236

Query: 122 LNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATKRSDENDEA 181
                +P IP ELS QG+DF+ KCFV++ K RWTA+MLL HP+++ + D      E D  
Sbjct: 237 GVGDEVPMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFV 292

Query: 182 TKRSDEN 183
                E+
Sbjct: 297 VNMKTED 292

[Close]

BLAST of Lsi02G024830 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 126.7 bits (317), Expect = 2.2e-29
Identity = 73/184 (39.67%), Postives = 109/184 (59.24%), Query Frame = 0


Query: 2   ILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQR 61
           +L+GL  IH+KGF H D+K +NIL+F   +    +KIADFG+  R      D D+    R
Sbjct: 115 VLRGLRHIHAKGFAHCDIKLANILLFNDGS----VKIADFGLAMRV-----DGDLT-ALR 174

Query: 62  GPLKFKRTPIYVSPESLVFND---AHDIWSLGCILVKMVSGKSAW---DDCSDEQLMIRL 121
             ++ + TP+Y++PE +  N+   A D+W+LGC +V+M SGK+AW   +      L+IR+
Sbjct: 175 KSVEIRGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRI 234

Query: 122 LNNKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLS-------EENDATKR 173
                +P IP  LS +GKDF+ KCFV++   RWTA+MLL H +++        EN   K 
Sbjct: 235 GVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKV 288

[Close]

BLAST of Lsi02G024830 vs. TAIR 10
Match: AT1G63700.1 (Protein kinase superfamily protein )

HSP 1 Score: 119.4 bits (298), Expect = 3.5e-27
Identity = 64/162 (39.51%), Postives = 92/162 (56.79%), Query Frame = 0


Query: 2   ILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQR 61
           IL GL+ +H+K  VH D+K +NILV P      ++K+ADFG+ +              Q 
Sbjct: 509 ILSGLAYLHAKNTVHRDIKGANILVDPHG----RVKVADFGMAKHIT----------AQS 568

Query: 62  GPLKFKRTPIYVSPE----SLVFNDAHDIWSLGCILVKMVSGKSAWDDCSDEQLMIRLLN 121
           GPL FK +P +++PE    S   N A DIWSLGC +++M + K  W        M ++ N
Sbjct: 569 GPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFKIGN 628

Query: 122 NKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYL 160
           +K +P IP  LS +GKDF++KC  RN  +R TA  LL H ++
Sbjct: 629 SKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

[Close]

BLAST of Lsi02G024830 vs. TAIR 10
Match: AT5G27510.1 (Protein kinase superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 4.6e-27
Identity = 71/170 (41.76%), Postives = 93/170 (54.71%), Query Frame = 0


Query: 1   MILKGLSCIHSKGFVHVDLKPSNILVFPQSNGKMKLKIADFGIGERCKHRDDDQDIKYRQ 60
           MIL+GL  IHS G+VH DLKP N+LVFP      ++KI+DFG+  +     D   I+Y  
Sbjct: 116 MILQGLVSIHSHGYVHCDLKPENVLVFP-CGDSYEVKISDFGLSLQVGEVPDHWKIEYPF 175

Query: 61  RGPLKFKRTPIYVSPESL---VFNDAHDIWSLGCILVKMVSGKSAWDDCSDEQLMIRLLN 120
            G L       Y+ PESL   V N   D+WSLGC++++M   K  W     E   + +L+
Sbjct: 176 VGTLN------YMPPESLHDGVANKTLDLWSLGCLVLEMYVCKKPWIGFIPED-FVYILS 235

Query: 121 NKNIPTIPIELSNQGKDFIKKCFVRNYKHRWTADMLLQHPYLSEENDATK 168
           N N P IP  L    + FI+KCF RN K R TA  LL H +L +E    K
Sbjct: 236 NGNPPEIPESLPCDARAFIQKCFSRNPKERGTASELLSHRFLRQEKSKLK 277

The following BLAST results are available for this feature:

BLAST of Lsi02G024830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	3.1e-28	39.67	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
Q9CAD5	5.0e-26	39.51	Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3...	[more]
F4HRJ4	1.7e-21	36.22	Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702...	[more]
Q9C5H5	6.3e-21	35.36	Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702...	[more]
O80888	3.1e-20	31.40	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]

BLAST of Lsi02G024830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DR57	1.3e-66	69.23	Mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo var. ma...	[more]
A0A1S3CQQ0	1.3e-66	69.23	mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo OX=3656...	[more]
A0A0A0KAC2	2.1e-64	68.82	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378...	[more]
A0A0A0K7G3	1.2e-62	67.21	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G065...	[more]
A0A5D3C4R6	3.4e-62	70.05	Mitogen-activated protein kinase kinase kinase A-like OS=Cucumis melo var. makuw...	[more]

BLAST of Lsi02G024830 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896325.1	1.6e-69	75.43	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
KAA0059705.1	2.8e-66	69.23	mitogen-activated protein kinase kinase kinase ANP1-like [Cucumis melo var. maku...	[more]
XP_008465601.1	2.8e-66	69.23	PREDICTED: mitogen-activated protein kinase kinase kinase ANP1-like, partial [Cu...	[more]
XP_031745125.1	4.4e-64	68.82	mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN44756.1 ...	[more]
XP_004137063.1	2.4e-62	67.21	mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN43756.1 ...	[more]

BLAST of Lsi02G024830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	9.6e-33	41.36	mitogen-activated protein kinase kinase kinase 21	[more]
AT5G67080.1	5.3e-31	39.57	mitogen-activated protein kinase kinase kinase 19	[more]
AT3G50310.1	2.2e-29	39.67	mitogen-activated protein kinase kinase kinase 20	[more]
AT1G63700.1	3.5e-27	39.51	Protein kinase superfamily protein	[more]
AT5G27510.1	4.6e-27	41.76	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 1..159 e-value: 2.5E-8 score: -16.1
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 2..159 e-value: 1.2E-31 score: 110.1
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..159 score: 27.099838
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 1..210 e-value: 3.8E-40 score: 140.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 163..188
None	No IPR available	PANTHER	PTHR48011:SF51	PROTEIN KINASE SUPERFAMILY PROTEIN	coord: 1..182
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 1..182
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 14..26
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 2..166

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G024830.1	Lsi02G024830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity