Lsi02G024350 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G024350 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 3.2e-186
Identity = 336/573 (58.64%), Postives = 412/573 (71.90%), Query Frame = 0

Query: 15  YSLILISLYITTSAAP------TGSACSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLS 74
           + LI +S ++ +  A       + + C +T DP YCKSV P+  GNV  YG  S RKSLS
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 75  SSQKFLRLVEKYLRSRSSLTVPAV-RALEDCQLLAGLNIDYLTTSFRAVNSTN-----RT 134
            S+KF+R V++Y++  + L+ PAV RAL+DC+ LAGL +DYL TSF  VN T+     + 
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 135 LPEMKADDVQSLLSAILTNQQTCLDGIKATA---GSWSLKNGLSLPLATDTKLYSLSLAF 194
           L   KADD+Q+LLSA LTN+QTCL+G+   A    +W+++ G++LPL  DTKL  +SLA 
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 195 FTKGWVPKKKKKPTWKAAGRQGG----------FRNGRMSLKMSSRTKAIYETATRRNLL 254
           FTKGWVPKKKK+  +  A  + G          FRNG + LKM+ +TKA+YE+ +RR L 
Sbjct: 186 FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLA 245

Query: 255 QTDDGGD----DQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVY 314
             D  GD      + + DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V+AG+Y
Sbjct: 246 DGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIY 305

Query: 315 EEYVLIAKNKRYLMMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNT 374
           EEY+ IAKNKRY+MMIGDGINQTVVT                 AV  P FVAVN+TFRNT
Sbjct: 306 EEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT 365

Query: 375 AGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV 434
           AG  KHQAVA+R+GAD S FY CSFEAYQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV
Sbjct: 366 AGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 425

Query: 435 FQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGR 494
           FQNCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  +S   VKT+LGR
Sbjct: 426 FQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLGR 485

Query: 495 PWKEYSRTVYMQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFH 542
           PWKEYSRTVYMQS++D  + P GWR W+GDFAL+T YYAE+ N GPGSNT+ RV WPG+H
Sbjct: 486 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 545

BLAST of Lsi02G024350 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 636.0 bits (1639), Expect = 4.0e-181
Identity = 336/561 (59.89%), Postives = 415/561 (73.98%), Query Frame = 0

Query: 17  LILISLYITT-SAAPTGSA---CSSTRDPSYCKSVLPNQT-GNVYSYGRSSFRKSLSSSQ 76
           L L +L+I + +  P+ S+   C +T DP +CKSV P  + G+V  YGR S RKSL+ S+
Sbjct: 13  LSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQSR 72

Query: 77  KFLRLVEKYL-RSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST--NRTLPEMKA 136
           KF R +++YL R+ + L+  AV AL+DC+ LA L  DYL TSF  VN T  ++TL   KA
Sbjct: 73  KFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSKA 132

Query: 137 DDVQSLLSAILTNQQTCLDGIK-ATAGSWSLKNGLSLPLATDTKLYSLSLAFFTKGWVPK 196
           D++Q+LLSA LTN+QTCLDGI  A + SW+++NG++LPL  DTKL+S+SLA FTKGWVPK
Sbjct: 133 DEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWVPK 192

Query: 197 KKK----------KPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDD 256
           KKK          K T         FRNG + LKM+  T+A+YE+ +RR L   DD   +
Sbjct: 193 KKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD-DDNDVN 252

Query: 257 QIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRY 316
            + V DIV V+Q+G+GNFTTI EA+ +APN +  T GYF+I+V++GVYEE V+IAKNKRY
Sbjct: 253 TVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRY 312

Query: 317 LMMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNTAGAIKHQAVAVR 376
           LMMIGDGIN+TVVT                 AV  P FVAVNMTFRNTAG  KHQAVA+R
Sbjct: 313 LMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMR 372

Query: 377 NGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM 436
           + ADLS FY CSFEAYQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+YPR PM
Sbjct: 373 SSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPM 432

Query: 437 SNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQ 496
            NQFNAITAQGRTDPNQNTG SI+NC I  ADDL  +S   VKT+LGRPWKEYSRTV+MQ
Sbjct: 433 QNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEYSRTVFMQ 492

Query: 497 SFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTA 542
           S++D+++ P GWR W+GDFAL+T YYAE+ N G GS+T++RV WPG+H+IN TDA NFT 
Sbjct: 493 SYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTV 552

BLAST of Lsi02G024350 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 607.1 bits (1564), Expect = 2.0e-172
Identity = 306/537 (56.98%), Postives = 391/537 (72.81%), Query Frame = 0

Query: 27  SAAPTGSACSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLRSRSS 86
           +A    + C    DPSYC+SVLPNQ G++YSYGR S R+SLS +++F+ +++  L  +  
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 87  LTVPA-VRALEDCQLLAGLNIDYLTTSFRAVNSTNRTLPEMKADDVQSLLSAILTNQQTC 146
           +   + V ALEDC+ LA L +DYL +S +  +ST +TL   +A+DV + LSA +TN+QTC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 147 LDGIKATAGSWSLKNGLSLPLATDTKLYSLSLAFFTKGWVPKKKK-KPTWKAAGRQG--- 206
           L+G+K+TA     +NGLS  L  DTKLY +SLA F+KGWVP++++ +P W+   R     
Sbjct: 151 LEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 207 GFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQIAVRDIVVVSQDGSGNFTTINEA 266
           GFRNG++ LKM+ R +A+Y T TRR LLQ+D    D + V DIV V Q+G+GNFTTIN A
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQVSDIVTVIQNGTGNFTTINAA 270

Query: 267 IAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMMIGDGINQTVVTA--------- 326
           IAAAPN +  ++GYFLI+V+AG+YEEYV + KNKRY+MMIGDGINQTV+T          
Sbjct: 271 IAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWT 330

Query: 327 --------VVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHS 386
                   + GP F+ VN+T RNTAG  K QAVA+R+G DLS FY CSFEAYQDTLYTHS
Sbjct: 331 TFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHS 390

Query: 387 LRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIY 446
           LRQFYR+CD+YGTVDFIFGNAAVV QNCN+YPR P   Q N +TAQGRTDPNQNTGT+I+
Sbjct: 391 LRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIH 450

Query: 447 NCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPAGWRAWDGDFALNTS 506
            C I  ADDL   S   VKT+LGRPWKEYSRTV MQ+++D  + P+GW AW GDFAL+T 
Sbjct: 451 GCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTL 510

Query: 507 YYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 542
           YYAE+ N GPGS+T+ RV WPG+H+IN TDA NFT  NF++ + W+ QTGVP+  GL
Sbjct: 511 YYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGL 558

BLAST of Lsi02G024350 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 2.4e-125
Identity = 245/540 (45.37%), Postives = 329/540 (60.93%), Query Frame = 0

Query: 34  ACSSTRDPSYCKSVL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLR----SRSS 93
           AC ST  P  C+++L    +   + Y YG+ + ++ L  + +  +++  Y R       S
Sbjct: 82  ACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGS 141

Query: 94  LTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTNRTLPEMKADDVQSLLSAILTNQQTCL 153
            T   + A+ DC  L+ L+++YL T    +  T + +     + V SLLS ++TNQQTCL
Sbjct: 142 ATAEEIGAVADCGELSELSVNYLETVTTEL-KTAQVMTAALVEHVNSLLSGVVTNQQTCL 201

Query: 154 DGIKATAGSWSLKNGLSLPLATDTKLYSLSLAFFTKGWVPKKKKKPTWKAAGRQGGFRNG 213
           DG+      ++    +  P+   T+LYS+SL   +       K+    K     GG  N 
Sbjct: 202 DGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILGGG--NS 261

Query: 214 RMSLKMSSRTKAIYETA--------TRRNLLQTDDGGDDQIAVRDIVVVSQDGSGNFTTI 273
                + +  K + +T         T RNL +  +     I V   V+V    S NFTTI
Sbjct: 262 TYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDNFTTI 321

Query: 274 NEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMMIGDGINQTVVT------- 333
            +AIAAAPNN+ P DGYF+I+   GVYEEY+++  NK+ LM++GDGIN+T++T       
Sbjct: 322 TDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHNVVD 381

Query: 334 ----------AVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLY 393
                     AVVG  F+AV++TFRNTAG  KHQAVA+RN A+ S+FY CSFE YQDTLY
Sbjct: 382 GWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLY 441

Query: 394 THSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGT 453
            HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PM+ Q NAITA GR DPNQNTG 
Sbjct: 442 VHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTGI 501

Query: 454 SIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPAGWRAWDGDFAL 513
           SI NC I AA DL     + + TFLGRPWK YSRTV+MQS++ D++ P GW  W+G   L
Sbjct: 502 SIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTIGL 561

Query: 514 NTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 542
           +T YY E+ NFGPG+NT++RV W G++L+N  +A NFT  NF + D WLPQT +P+  GL
Sbjct: 562 DTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIPFYGGL 615

BLAST of Lsi02G024350 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 7.7e-124
Identity = 247/547 (45.16%), Postives = 331/547 (60.51%), Query Frame = 0

Query: 27  SAAPTGSACSSTRDPSYCKSVL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLR- 86
           S  P   AC ST  P  C+++L    +   + Y YG+ + ++ L  +++  +++ ++ + 
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 87  ---SRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTNRTLPEMKADDVQSLLSAIL 146
                 + TV  V A+ DC  LA L+++YL T    + +    +     D V SLL  ++
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAA-ELMTAALVDRVTSLLGGVV 199

Query: 147 TNQQTCLDGIKATAGSWSLKNGLSLPLATDTKLYSLSLAFFTKGWVPKKKKKPTWKAAGR 206
           TNQQTCLDG+      ++    +  PL   T+LYS+SL   +       K+    K  G+
Sbjct: 200 TNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSK--GK 259

Query: 207 QGGFRNGRMSLKMSSRTKAIYETATR--------RNLLQTDDGGDDQIAVRDIVVVSQDG 266
             G  N  +   + +  K + +T  +        RNL +  +     I VR+ V V    
Sbjct: 260 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 319

Query: 267 SGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMMIGDGINQTVVT 326
           + NF TI EA+AAAPN++ P  GYF+I+  AG+YEEYV+I+  KR +M+IGDGIN+T+++
Sbjct: 320 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 379

Query: 327 -----------------AVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFE 386
                            AVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY CSFE
Sbjct: 380 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 439

Query: 387 AYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTD 446
            YQDTLY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PM+NQ NA+TA GRTD
Sbjct: 440 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 499

Query: 447 PNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPAGWRA 506
           PNQ TG SI NC I AA DL  +      TFLGRPWK YSRTVY+QS++ D++ P GW  
Sbjct: 500 PNQKTGISIINCTIGAAPDLAADP-KSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 559

Query: 507 WDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTG 542
           W+G   L+T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D WLPQT 
Sbjct: 560 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTD 619

BLAST of Lsi02G024350 vs. ExPASy TrEMBL
Match: A0A5D3D3H4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001320 PE=3 SV=1)

HSP 1 Score: 1005.4 bits (2598), Expect = 9.3e-290
Identity = 514/562 (91.46%), Postives = 525/562 (93.42%), Query Frame = 0

Query: 1   MAAGIITTAAAAAFYSLILISLYITTSAAPTGSACSSTRDPSYCKSVLPNQTGNVYSYGR 60
           MAAG      AAAFY LILISLYITTSA+PTGS CSST DPSYCKS LPNQTGNVYSYGR
Sbjct: 1   MAAG----TTAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGR 60

Query: 61  SSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTN 120
           SSFRKSLSSSQKFLRLVEK+LRSRSSLTV AVRALEDCQLLAGLNIDYL TSF+AVN+T+
Sbjct: 61  SSFRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATS 120

Query: 121 RTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLATDTKLYSLSLAFF 180
           R L EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFF
Sbjct: 121 RALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFF 180

Query: 181 TKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDD--GGDDQ 240
           TKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRT+AIYETATRRNLLQTDD  G DDQ
Sbjct: 181 TKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYL 300
           IAVRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYV IAKNKRYL
Sbjct: 241 IAVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYL 300

Query: 301 MMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQT+VT                 AVVGPGFVAVNMTFRNTAGAIKHQAVA+RN
Sbjct: 301 MMIGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
           GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS
Sbjct: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSIYNCRITAADDL NNSGAGVKTFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 544
           NFVLADDWLPQTGVPYTSGLT+
Sbjct: 541 NFVLADDWLPQTGVPYTSGLTE 558

BLAST of Lsi02G024350 vs. ExPASy TrEMBL
Match: A0A1S3BSI2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1)

HSP 1 Score: 1005.4 bits (2598), Expect = 9.3e-290
Identity = 514/562 (91.46%), Postives = 525/562 (93.42%), Query Frame = 0

Query: 1   MAAGIITTAAAAAFYSLILISLYITTSAAPTGSACSSTRDPSYCKSVLPNQTGNVYSYGR 60
           MAAG      AAAFY LILISLYITTSA+PTGS CSST DPSYCKS LPNQTGNVYSYGR
Sbjct: 1   MAAG----TTAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGR 60

Query: 61  SSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTN 120
           SSFRKSLSSSQKFLRLVEK+LRSRSSLTV AVRALEDCQLLAGLNIDYL TSF+AVN+T+
Sbjct: 61  SSFRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATS 120

Query: 121 RTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLATDTKLYSLSLAFF 180
           R L EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFF
Sbjct: 121 RALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFF 180

Query: 181 TKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDD--GGDDQ 240
           TKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRT+AIYETATRRNLLQTDD  G DDQ
Sbjct: 181 TKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYL 300
           IAVRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYV IAKNKRYL
Sbjct: 241 IAVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYL 300

Query: 301 MMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQT+VT                 AVVGPGFVAVNMTFRNTAGAIKHQAVA+RN
Sbjct: 301 MMIGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
           GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS
Sbjct: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSIYNCRITAADDL NNSGAGVKTFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 544
           NFVLADDWLPQTGVPYTSGLT+
Sbjct: 541 NFVLADDWLPQTGVPYTSGLTE 558

BLAST of Lsi02G024350 vs. ExPASy TrEMBL
Match: A0A0A0KB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405820 PE=3 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 2.8e-286
Identity = 511/562 (90.93%), Postives = 522/562 (92.88%), Query Frame = 0

Query: 1   MAAGIITTAAAAAFY-SLILISLYITTSAAPTGSACSSTRDPSYCKSVLPNQTGNVYSYG 60
           MAAG  TTA AAAFY  LILISLYITTSA+PTGS CSST DPSYCKS LPNQTGNVYSYG
Sbjct: 1   MAAG--TTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYG 60

Query: 61  RSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST 120
           RSSFRKSLSSSQKFLRLVEK+LRSRSSLTVPAVRALEDC LLAGLNIDYL TSF+ VN+T
Sbjct: 61  RSSFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALEDCLLLAGLNIDYLKTSFQTVNTT 120

Query: 121 NRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLATDTKLYSLSLAF 180
           +R L EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAF
Sbjct: 121 SRVLTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAF 180

Query: 181 FTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGG-DDQ 240
           FTKGWVPKKKK+PTWKAAGRQGGFRNGRMSLKMSSRT+AIYE ATRRNLLQTDDGG DDQ
Sbjct: 181 FTKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGGDDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYL 300
           I VRDIVVVSQDGSGNFTTINEAIAAA NNSAPTDGYFLIFVSAGVYEEYVL+AKNKRYL
Sbjct: 241 IKVRDIVVVSQDGSGNFTTINEAIAAATNNSAPTDGYFLIFVSAGVYEEYVLVAKNKRYL 300

Query: 301 MMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQT+VT                 AVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
           GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS
Sbjct: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSIYNCRITAADDL NNS AGVKTFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSDAGVKTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERV W GFHLINDTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWAGFHLINDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 544
           NFVLADDWLPQTGVPY SGLT+
Sbjct: 541 NFVLADDWLPQTGVPYDSGLTE 560

BLAST of Lsi02G024350 vs. ExPASy TrEMBL
Match: A0A6J1GQE6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 3.5e-281
Identity = 500/565 (88.50%), Postives = 515/565 (91.15%), Query Frame = 0

Query: 1   MAAGIITTAAAAAFYSLILISLYIT-----TSAAPTGSACSSTRDPSYCKSVLPNQTGNV 60
           MAA   TT   AAFYSL+LISLYIT     TSAAPTGS CSS  DPSYCKS LPNQTGNV
Sbjct: 1   MAAAPPTT---AAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNV 60

Query: 61  YSYGRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRA 120
           YSYGRSS RKSLSSSQKFLRLVEKYLRSRSSLTV AVRALEDCQLLAGLNIDYLTTSFR 
Sbjct: 61  YSYGRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRT 120

Query: 121 VNSTNRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLATDTKLYSL 180
           VNST+ TLPEMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPLA DTKLYS+
Sbjct: 121 VNSTSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSM 180

Query: 181 SLAFFTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGG 240
           SLAFFTKGWVPKKKKKPTW +AGRQGGF NGRMSLKMSSRTKAIYETATRRNLLQT+DGG
Sbjct: 181 SLAFFTKGWVPKKKKKPTWTSAGRQGGFNNGRMSLKMSSRTKAIYETATRRNLLQTEDGG 240

Query: 241 DDQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNK 300
           DDQI+VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV IAKNK
Sbjct: 241 DDQISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNK 300

Query: 301 RYLMMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNTAGAIKHQAVA 360
           RYLMMIGDGINQTVVT                 AVVGPGFVAVNMTFRN AGAIKHQAVA
Sbjct: 301 RYLMMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNAAGAIKHQAVA 360

Query: 361 VRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRL 420
           VRN ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRL
Sbjct: 361 VRNSADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRL 420

Query: 421 PMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVY 480
           PM+NQFNAITAQGRTDPNQNTGTSI+NCRITAADDL NNSGA V TFLGRPWKEYSRTVY
Sbjct: 421 PMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVY 480

Query: 481 MQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNF 540
           MQSFMDDLINPAGWRAWDGDFALNT YYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNF
Sbjct: 481 MQSFMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNF 540

Query: 541 TAGNFVLADDWLPQTGVPYTSGLTD 544
           TAGNF++ADDW+PQTGVPYTSGLT+
Sbjct: 541 TAGNFLMADDWVPQTGVPYTSGLTE 562

BLAST of Lsi02G024350 vs. ExPASy TrEMBL
Match: A0A6J1JVJ5 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.8e-277
Identity = 494/565 (87.43%), Postives = 511/565 (90.44%), Query Frame = 0

Query: 1   MAAGIITTAAAAAFYSLILISLYIT-----TSAAPTGSACSSTRDPSYCKSVLPNQTGNV 60
           MAA   TT    AFY L+LISLYIT     TSAAPTGS CSS  DPSYCKS LPNQTGNV
Sbjct: 1   MAAAPPTT---PAFYYLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNV 60

Query: 61  YSYGRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRA 120
           YSYGRSS RKSLSSSQKFLRLVEKYLRSRSSLTV AVR+LEDCQLLAGLNIDYLTTSFR 
Sbjct: 61  YSYGRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRSLEDCQLLAGLNIDYLTTSFRT 120

Query: 121 VNSTNRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLATDTKLYSL 180
           VNST+ TLPEMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPL  DTKLYS+
Sbjct: 121 VNSTSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLDADTKLYSM 180

Query: 181 SLAFFTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGG 240
           SLAFFTKGWVPKKKKK TW +AGRQGGF NGRMS KMSSRTKAIYE ATRRNLLQTDDGG
Sbjct: 181 SLAFFTKGWVPKKKKKQTWTSAGRQGGFSNGRMSFKMSSRTKAIYEMATRRNLLQTDDGG 240

Query: 241 DDQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNK 300
           DDQI+VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV IAKNK
Sbjct: 241 DDQISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNK 300

Query: 301 RYLMMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNTAGAIKHQAVA 360
           RYLMMIGDGINQTVVT                 AVVGPGFVAVNMTFRNTAGAIKHQAVA
Sbjct: 301 RYLMMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA 360

Query: 361 VRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRL 420
           VRN ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRL
Sbjct: 361 VRNSADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRL 420

Query: 421 PMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVY 480
           PM+NQFNAITAQGRTDPNQNTGTSI+NCRITAADDL NN+GA V TFLGRPWKEYSRTVY
Sbjct: 421 PMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGARVNTFLGRPWKEYSRTVY 480

Query: 481 MQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNF 540
           MQSFM+DLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNF
Sbjct: 481 MQSFMNDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNF 540

Query: 541 TAGNFVLADDWLPQTGVPYTSGLTD 544
           TAGNF++ADDW+PQTGVPYTSGLT+
Sbjct: 541 TAGNFLMADDWVPQTGVPYTSGLTE 562

BLAST of Lsi02G024350 vs. NCBI nr
Match: XP_038896831.1 (probable pectinesterase/pectinesterase inhibitor 7 [Benincasa hispida])

HSP 1 Score: 1033.9 bits (2672), Expect = 5.0e-298
Identity = 527/561 (93.94%), Postives = 532/561 (94.83%), Query Frame = 0

Query: 1   MAAGIITTA-AAAAFYSLILISLYITTSAAPTGSACSSTRDPSYCKSVLPNQTGNVYSYG 60
           MAAG I  A AAAAFY LILISLYITTSAAPTGS CSSTRDPSYCKSVLPNQTGNVYSYG
Sbjct: 1   MAAGTIPAATAAAAFYFLILISLYITTSAAPTGSECSSTRDPSYCKSVLPNQTGNVYSYG 60

Query: 61  RSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST 120
           RSSFRKSLS+SQKFLRLVEK+LRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST
Sbjct: 61  RSSFRKSLSASQKFLRLVEKHLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST 120

Query: 121 NRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLATDTKLYSLSLAF 180
           +RTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLA DTKLYSLSLAF
Sbjct: 121 SRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAF 180

Query: 181 FTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQI 240
           FTKGWVPKKKKKPTWKAAGR GGFRNGRMSLKMSSR KAIYETATRRNLLQ DDGGDDQI
Sbjct: 181 FTKGWVPKKKKKPTWKAAGRHGGFRNGRMSLKMSSRAKAIYETATRRNLLQADDGGDDQI 240

Query: 241 AVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLM 300
           AVRDIVVVSQDGSGNFTTINEA+AAAPNNSAPTDGYFLIFVSAGVYEEYV IAKNKRYLM
Sbjct: 241 AVRDIVVVSQDGSGNFTTINEAVAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLM 300

Query: 301 MIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNTAGAIKHQAVAVRNG 360
           MIGDGINQTVVT                 AVVGPGFVAVNMTFRNTAGAIKHQAVAVRNG
Sbjct: 301 MIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNG 360

Query: 361 ADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSN 420
           ADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSN
Sbjct: 361 ADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSN 420

Query: 421 QFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQSF 480
           QFNAITAQGRTDPNQNTGTSIYNCRITAADDL NN+GAGVKTFLGRPWKEYSRTVYMQSF
Sbjct: 421 QFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNTGAGVKTFLGRPWKEYSRTVYMQSF 480

Query: 481 MDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGN 540
           MDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGN
Sbjct: 481 MDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGN 540

Query: 541 FVLADDWLPQTGVPYTSGLTD 544
           FVLADDWLPQTGVPYTSGLTD
Sbjct: 541 FVLADDWLPQTGVPYTSGLTD 561

BLAST of Lsi02G024350 vs. NCBI nr
Match: XP_008451544.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Cucumis melo] >KAA0046659.1 putative pectinesterase/pectinesterase inhibitor 7 [Cucumis melo var. makuwa] >TYK18194.1 putative pectinesterase/pectinesterase inhibitor 7 [Cucumis melo var. makuwa])

HSP 1 Score: 1005.4 bits (2598), Expect = 1.9e-289
Identity = 514/562 (91.46%), Postives = 525/562 (93.42%), Query Frame = 0

Query: 1   MAAGIITTAAAAAFYSLILISLYITTSAAPTGSACSSTRDPSYCKSVLPNQTGNVYSYGR 60
           MAAG      AAAFY LILISLYITTSA+PTGS CSST DPSYCKS LPNQTGNVYSYGR
Sbjct: 1   MAAG----TTAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGR 60

Query: 61  SSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTN 120
           SSFRKSLSSSQKFLRLVEK+LRSRSSLTV AVRALEDCQLLAGLNIDYL TSF+AVN+T+
Sbjct: 61  SSFRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATS 120

Query: 121 RTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLATDTKLYSLSLAFF 180
           R L EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFF
Sbjct: 121 RALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFF 180

Query: 181 TKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDD--GGDDQ 240
           TKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRT+AIYETATRRNLLQTDD  G DDQ
Sbjct: 181 TKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYL 300
           IAVRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYV IAKNKRYL
Sbjct: 241 IAVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYL 300

Query: 301 MMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQT+VT                 AVVGPGFVAVNMTFRNTAGAIKHQAVA+RN
Sbjct: 301 MMIGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
           GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS
Sbjct: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSIYNCRITAADDL NNSGAGVKTFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 544
           NFVLADDWLPQTGVPYTSGLT+
Sbjct: 541 NFVLADDWLPQTGVPYTSGLTE 558

BLAST of Lsi02G024350 vs. NCBI nr
Match: XP_004136013.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucumis sativus] >KGN44976.1 hypothetical protein Csa_016133 [Cucumis sativus])

HSP 1 Score: 993.8 bits (2568), Expect = 5.8e-286
Identity = 511/562 (90.93%), Postives = 522/562 (92.88%), Query Frame = 0

Query: 1   MAAGIITTAAAAAFY-SLILISLYITTSAAPTGSACSSTRDPSYCKSVLPNQTGNVYSYG 60
           MAAG  TTA AAAFY  LILISLYITTSA+PTGS CSST DPSYCKS LPNQTGNVYSYG
Sbjct: 1   MAAG--TTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYG 60

Query: 61  RSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST 120
           RSSFRKSLSSSQKFLRLVEK+LRSRSSLTVPAVRALEDC LLAGLNIDYL TSF+ VN+T
Sbjct: 61  RSSFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALEDCLLLAGLNIDYLKTSFQTVNTT 120

Query: 121 NRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLATDTKLYSLSLAF 180
           +R L EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAF
Sbjct: 121 SRVLTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAF 180

Query: 181 FTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGG-DDQ 240
           FTKGWVPKKKK+PTWKAAGRQGGFRNGRMSLKMSSRT+AIYE ATRRNLLQTDDGG DDQ
Sbjct: 181 FTKGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGGDDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYL 300
           I VRDIVVVSQDGSGNFTTINEAIAAA NNSAPTDGYFLIFVSAGVYEEYVL+AKNKRYL
Sbjct: 241 IKVRDIVVVSQDGSGNFTTINEAIAAATNNSAPTDGYFLIFVSAGVYEEYVLVAKNKRYL 300

Query: 301 MMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQT+VT                 AVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
           GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS
Sbjct: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSIYNCRITAADDL NNS AGVKTFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSDAGVKTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERV W GFHLINDTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWAGFHLINDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 544
           NFVLADDWLPQTGVPY SGLT+
Sbjct: 541 NFVLADDWLPQTGVPYDSGLTE 560

BLAST of Lsi02G024350 vs. NCBI nr
Match: KAG7014346.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 978.8 bits (2529), Expect = 1.9e-281
Identity = 501/565 (88.67%), Postives = 516/565 (91.33%), Query Frame = 0

Query: 1   MAAGIITTAAAAAFYSLILISLYIT-----TSAAPTGSACSSTRDPSYCKSVLPNQTGNV 60
           MAA   TT   AAFYSL+LISLYIT     TSAAPTGS CSS  DPSYCKS LPNQTGNV
Sbjct: 1   MAAAPPTT---AAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNV 60

Query: 61  YSYGRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRA 120
           YSYGRSS RKSLSSSQKFLRLVEKYLRSRSSLTV AVRALEDCQLLAGLNIDYLTTSFR 
Sbjct: 61  YSYGRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRT 120

Query: 121 VNSTNRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLATDTKLYSL 180
           VNST+ TLPEMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPLA DTKLYS+
Sbjct: 121 VNSTSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSM 180

Query: 181 SLAFFTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGG 240
           SLAFFTKGWVPKKKKKPTW +AGRQGGF NGRMSLKMSSRTKAIYETATRRNLLQTDDGG
Sbjct: 181 SLAFFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGG 240

Query: 241 DDQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNK 300
           DDQI+VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV IAKNK
Sbjct: 241 DDQISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNK 300

Query: 301 RYLMMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNTAGAIKHQAVA 360
           RYLMMIGDGINQTVVT                 AVVGPGFVAVNMTFRNTAGAIKHQAVA
Sbjct: 301 RYLMMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA 360

Query: 361 VRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRL 420
           VRN ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRL
Sbjct: 361 VRNSADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRL 420

Query: 421 PMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVY 480
           PM+NQFNAITAQGRTDPNQNTGTSI+NCRITAADDL NNSGA V TFLGRPWKEYSRTVY
Sbjct: 421 PMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVY 480

Query: 481 MQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNF 540
           MQSFMDDLINPAGWRAWDGDFALNT YYAEFGNFGPGSNT+ERVNW GFHLI+DTDAGNF
Sbjct: 481 MQSFMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNF 540

Query: 541 TAGNFVLADDWLPQTGVPYTSGLTD 544
           TAGNF++ADDW+PQTGVPYTSGLT+
Sbjct: 541 TAGNFLMADDWVPQTGVPYTSGLTE 562

BLAST of Lsi02G024350 vs. NCBI nr
Match: KAG6575804.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 978.4 bits (2528), Expect = 2.5e-281
Identity = 501/565 (88.67%), Postives = 516/565 (91.33%), Query Frame = 0

Query: 1   MAAGIITTAAAAAFYSLILISLYIT-----TSAAPTGSACSSTRDPSYCKSVLPNQTGNV 60
           MAA   TT   AAFYSL+LISLYIT     TSAAPTGS CSS  DPSYCKS LPNQTGNV
Sbjct: 1   MAAAPPTT---AAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNV 60

Query: 61  YSYGRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRA 120
           YSYGRSS RKSLSSSQKFLRLVEKYLRSRSSLTV AVRALEDCQLLAGLNIDYLTTSFR 
Sbjct: 61  YSYGRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRT 120

Query: 121 VNSTNRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLATDTKLYSL 180
           VNST+ TLPEMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPLA DTKLYS+
Sbjct: 121 VNSTSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSM 180

Query: 181 SLAFFTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGG 240
           SLAFFTKGWVPKKKKKPTW +AGRQGGF NGRMSLKMSSRTKAIYETATRRNLLQT+DGG
Sbjct: 181 SLAFFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTEDGG 240

Query: 241 DDQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNK 300
           DDQI+VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV IAKNK
Sbjct: 241 DDQISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNK 300

Query: 301 RYLMMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNTAGAIKHQAVA 360
           RYLMMIGDGINQTVVT                 AVVGPGFVAVNMTFRNTAGAIKHQAVA
Sbjct: 301 RYLMMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA 360

Query: 361 VRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRL 420
           VRN ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRL
Sbjct: 361 VRNSADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRL 420

Query: 421 PMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVY 480
           PM+NQFNAITAQGRTDPNQNTGTSI+NCRITAADDL NNSGA V TFLGRPWKEYSRTVY
Sbjct: 421 PMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVY 480

Query: 481 MQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNF 540
           MQSFMDDLINPAGWRAWDGDFALNT YYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNF
Sbjct: 481 MQSFMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNF 540

Query: 541 TAGNFVLADDWLPQTGVPYTSGLTD 544
           TAGNF++ADDW+PQTGVPYTSGLT+
Sbjct: 541 TAGNFLMADDWVPQTGVPYTSGLTE 562

BLAST of Lsi02G024350 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 652.9 bits (1683), Expect = 2.3e-187
Identity = 336/573 (58.64%), Postives = 412/573 (71.90%), Query Frame = 0

Query: 15  YSLILISLYITTSAAP------TGSACSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLS 74
           + LI +S ++ +  A       + + C +T DP YCKSV P+  GNV  YG  S RKSLS
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 75  SSQKFLRLVEKYLRSRSSLTVPAV-RALEDCQLLAGLNIDYLTTSFRAVNSTN-----RT 134
            S+KF+R V++Y++  + L+ PAV RAL+DC+ LAGL +DYL TSF  VN T+     + 
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 135 LPEMKADDVQSLLSAILTNQQTCLDGIKATA---GSWSLKNGLSLPLATDTKLYSLSLAF 194
           L   KADD+Q+LLSA LTN+QTCL+G+   A    +W+++ G++LPL  DTKL  +SLA 
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 195 FTKGWVPKKKKKPTWKAAGRQGG----------FRNGRMSLKMSSRTKAIYETATRRNLL 254
           FTKGWVPKKKK+  +  A  + G          FRNG + LKM+ +TKA+YE+ +RR L 
Sbjct: 186 FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLA 245

Query: 255 QTDDGGD----DQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVY 314
             D  GD      + + DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V+AG+Y
Sbjct: 246 DGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIY 305

Query: 315 EEYVLIAKNKRYLMMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNT 374
           EEY+ IAKNKRY+MMIGDGINQTVVT                 AV  P FVAVN+TFRNT
Sbjct: 306 EEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT 365

Query: 375 AGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV 434
           AG  KHQAVA+R+GAD S FY CSFEAYQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV
Sbjct: 366 AGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 425

Query: 435 FQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGR 494
           FQNCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  +S   VKT+LGR
Sbjct: 426 FQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLGR 485

Query: 495 PWKEYSRTVYMQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFH 542
           PWKEYSRTVYMQS++D  + P GWR W+GDFAL+T YYAE+ N GPGSNT+ RV WPG+H
Sbjct: 486 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 545

BLAST of Lsi02G024350 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 636.0 bits (1639), Expect = 2.9e-182
Identity = 336/561 (59.89%), Postives = 415/561 (73.98%), Query Frame = 0

Query: 17  LILISLYITT-SAAPTGSA---CSSTRDPSYCKSVLPNQT-GNVYSYGRSSFRKSLSSSQ 76
           L L +L+I + +  P+ S+   C +T DP +CKSV P  + G+V  YGR S RKSL+ S+
Sbjct: 13  LSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQSR 72

Query: 77  KFLRLVEKYL-RSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST--NRTLPEMKA 136
           KF R +++YL R+ + L+  AV AL+DC+ LA L  DYL TSF  VN T  ++TL   KA
Sbjct: 73  KFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSKA 132

Query: 137 DDVQSLLSAILTNQQTCLDGIK-ATAGSWSLKNGLSLPLATDTKLYSLSLAFFTKGWVPK 196
           D++Q+LLSA LTN+QTCLDGI  A + SW+++NG++LPL  DTKL+S+SLA FTKGWVPK
Sbjct: 133 DEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWVPK 192

Query: 197 KKK----------KPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDD 256
           KKK          K T         FRNG + LKM+  T+A+YE+ +RR L   DD   +
Sbjct: 193 KKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD-DDNDVN 252

Query: 257 QIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRY 316
            + V DIV V+Q+G+GNFTTI EA+ +APN +  T GYF+I+V++GVYEE V+IAKNKRY
Sbjct: 253 TVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRY 312

Query: 317 LMMIGDGINQTVVT-----------------AVVGPGFVAVNMTFRNTAGAIKHQAVAVR 376
           LMMIGDGIN+TVVT                 AV  P FVAVNMTFRNTAG  KHQAVA+R
Sbjct: 313 LMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMR 372

Query: 377 NGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM 436
           + ADLS FY CSFEAYQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+YPR PM
Sbjct: 373 SSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPM 432

Query: 437 SNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQ 496
            NQFNAITAQGRTDPNQNTG SI+NC I  ADDL  +S   VKT+LGRPWKEYSRTV+MQ
Sbjct: 433 QNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEYSRTVFMQ 492

Query: 497 SFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTA 542
           S++D+++ P GWR W+GDFAL+T YYAE+ N G GS+T++RV WPG+H+IN TDA NFT 
Sbjct: 493 SYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTV 552

BLAST of Lsi02G024350 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 607.1 bits (1564), Expect = 1.4e-173
Identity = 306/537 (56.98%), Postives = 391/537 (72.81%), Query Frame = 0

Query: 27  SAAPTGSACSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLRSRSS 86
           +A    + C    DPSYC+SVLPNQ G++YSYGR S R+SLS +++F+ +++  L  +  
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 87  LTVPA-VRALEDCQLLAGLNIDYLTTSFRAVNSTNRTLPEMKADDVQSLLSAILTNQQTC 146
           +   + V ALEDC+ LA L +DYL +S +  +ST +TL   +A+DV + LSA +TN+QTC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 147 LDGIKATAGSWSLKNGLSLPLATDTKLYSLSLAFFTKGWVPKKKK-KPTWKAAGRQG--- 206
           L+G+K+TA     +NGLS  L  DTKLY +SLA F+KGWVP++++ +P W+   R     
Sbjct: 151 LEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 207 GFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQIAVRDIVVVSQDGSGNFTTINEA 266
           GFRNG++ LKM+ R +A+Y T TRR LLQ+D    D + V DIV V Q+G+GNFTTIN A
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQVSDIVTVIQNGTGNFTTINAA 270

Query: 267 IAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMMIGDGINQTVVTA--------- 326
           IAAAPN +  ++GYFLI+V+AG+YEEYV + KNKRY+MMIGDGINQTV+T          
Sbjct: 271 IAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWT 330

Query: 327 --------VVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHS 386
                   + GP F+ VN+T RNTAG  K QAVA+R+G DLS FY CSFEAYQDTLYTHS
Sbjct: 331 TFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHS 390

Query: 387 LRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIY 446
           LRQFYR+CD+YGTVDFIFGNAAVV QNCN+YPR P   Q N +TAQGRTDPNQNTGT+I+
Sbjct: 391 LRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIH 450

Query: 447 NCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPAGWRAWDGDFALNTS 506
            C I  ADDL   S   VKT+LGRPWKEYSRTV MQ+++D  + P+GW AW GDFAL+T 
Sbjct: 451 GCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTL 510

Query: 507 YYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 542
           YYAE+ N GPGS+T+ RV WPG+H+IN TDA NFT  NF++ + W+ QTGVP+  GL
Sbjct: 511 YYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGL 558

BLAST of Lsi02G024350 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 450.7 bits (1158), Expect = 1.7e-126
Identity = 245/540 (45.37%), Postives = 329/540 (60.93%), Query Frame = 0

Query: 34  ACSSTRDPSYCKSVL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLR----SRSS 93
           AC ST  P  C+++L    +   + Y YG+ + ++ L  + +  +++  Y R       S
Sbjct: 82  ACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGS 141

Query: 94  LTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTNRTLPEMKADDVQSLLSAILTNQQTCL 153
            T   + A+ DC  L+ L+++YL T    +  T + +     + V SLLS ++TNQQTCL
Sbjct: 142 ATAEEIGAVADCGELSELSVNYLETVTTEL-KTAQVMTAALVEHVNSLLSGVVTNQQTCL 201

Query: 154 DGIKATAGSWSLKNGLSLPLATDTKLYSLSLAFFTKGWVPKKKKKPTWKAAGRQGGFRNG 213
           DG+      ++    +  P+   T+LYS+SL   +       K+    K     GG  N 
Sbjct: 202 DGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILGGG--NS 261

Query: 214 RMSLKMSSRTKAIYETA--------TRRNLLQTDDGGDDQIAVRDIVVVSQDGSGNFTTI 273
                + +  K + +T         T RNL +  +     I V   V+V    S NFTTI
Sbjct: 262 TYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDNFTTI 321

Query: 274 NEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMMIGDGINQTVVT------- 333
            +AIAAAPNN+ P DGYF+I+   GVYEEY+++  NK+ LM++GDGIN+T++T       
Sbjct: 322 TDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHNVVD 381

Query: 334 ----------AVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLY 393
                     AVVG  F+AV++TFRNTAG  KHQAVA+RN A+ S+FY CSFE YQDTLY
Sbjct: 382 GWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLY 441

Query: 394 THSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGT 453
            HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PM+ Q NAITA GR DPNQNTG 
Sbjct: 442 VHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTGI 501

Query: 454 SIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPAGWRAWDGDFAL 513
           SI NC I AA DL     + + TFLGRPWK YSRTV+MQS++ D++ P GW  W+G   L
Sbjct: 502 SIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTIGL 561

Query: 514 NTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 542
           +T YY E+ NFGPG+NT++RV W G++L+N  +A NFT  NF + D WLPQT +P+  GL
Sbjct: 562 DTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIPFYGGL 615

BLAST of Lsi02G024350 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 445.7 bits (1145), Expect = 5.5e-125
Identity = 247/547 (45.16%), Postives = 331/547 (60.51%), Query Frame = 0

Query: 27  SAAPTGSACSSTRDPSYCKSVL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLR- 86
           S  P   AC ST  P  C+++L    +   + Y YG+ + ++ L  +++  +++ ++ + 
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 87  ---SRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTNRTLPEMKADDVQSLLSAIL 146
                 + TV  V A+ DC  LA L+++YL T    + +    +     D V SLL  ++
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAA-ELMTAALVDRVTSLLGGVV 199

Query: 147 TNQQTCLDGIKATAGSWSLKNGLSLPLATDTKLYSLSLAFFTKGWVPKKKKKPTWKAAGR 206
           TNQQTCLDG+      ++    +  PL   T+LYS+SL   +       K+    K  G+
Sbjct: 200 TNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSK--GK 259

Query: 207 QGGFRNGRMSLKMSSRTKAIYETATR--------RNLLQTDDGGDDQIAVRDIVVVSQDG 266
             G  N  +   + +  K + +T  +        RNL +  +     I VR+ V V    
Sbjct: 260 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 319

Query: 267 SGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVLIAKNKRYLMMIGDGINQTVVT 326
           + NF TI EA+AAAPN++ P  GYF+I+  AG+YEEYV+I+  KR +M+IGDGIN+T+++
Sbjct: 320 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 379

Query: 327 -----------------AVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFE 386
                            AVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY CSFE
Sbjct: 380 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 439

Query: 387 AYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTD 446
            YQDTLY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PM+NQ NA+TA GRTD
Sbjct: 440 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 499

Query: 447 PNQNTGTSIYNCRITAADDLTNNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPAGWRA 506
           PNQ TG SI NC I AA DL  +      TFLGRPWK YSRTVY+QS++ D++ P GW  
Sbjct: 500 PNQKTGISIINCTIGAAPDLAADP-KSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 559

Query: 507 WDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTG 542
           W+G   L+T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D WLPQT 
Sbjct: 560 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTD 619